HO664306

EST Overview
NameHO664306
Unique NameHO664306
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length570
Libraries
Library NameType
LIBEST_026619 TDZ treated Chickpea SSH librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Homology
[Close]
BLAST of HO664306 vs. SwissProt
Match: SRS1_ORYSJ (Salt stress root protein RS1 OS=Oryza sativa subsp. japonica GN=Os01g0233000 PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.547e-8
Identity = 30/65 (46.15%), Postives = 45/65 (69.23%), Query Frame = 1 
Query:  271 QSLVKERDEAG-LKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            ++L+K++  A  +KKN   V KF+EEL KIDFPG+K VS+A +K G   +S  I ++ EKV+ F+
Sbjct:   65 KALLKDKKTASKIKKNGPAVTKFLEELAKIDFPGAKPVSDAVAKSGTTPLSPAIAFILEKVAPFV 129
[Close]
BLAST of HO664306 vs. SwissProt
Match: SRS1_ORYSI (Salt stress root protein RS1 OS=Oryza sativa subsp. indica GN=OsI_001009 PE=1 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.547e-8
Identity = 30/65 (46.15%), Postives = 45/65 (69.23%), Query Frame = 1 
Query:  271 QSLVKERDEAG-LKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            ++L+K++  A  +KKN   V KF+EEL KIDFPG+K VS+A +K G   +S  I ++ EKV+ F+
Sbjct:   65 KALLKDKKTASKIKKNGPAVTKFLEELAKIDFPGAKPVSDAVAKSGTTPLSPAIAFILEKVAPFV 129
[Close]
BLAST of HO664306 vs. TrEMBL
Match: Q9SMK5_CICAR (Plasma membrane intrinsic polypeptide OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 4.138e-27
Identity = 63/64 (98.44%), Postives = 64/64 (100.00%), Query Frame = 1 
Query:  271 QSLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            +SLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI
Sbjct:   68 KSLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 131
[Close]
BLAST of HO664306 vs. TrEMBL
Match: B7FH09_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 5.058e-25
Identity = 57/64 (89.06%), Postives = 64/64 (100.00%), Query Frame = 1 
Query:  271 QSLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            +SLVKERD+AGLKKN+TEV+KFIEELVKIDFPGSKAVSEASSKFGPAL+SGP+F+VFEKVSTFI
Sbjct:   67 KSLVKERDQAGLKKNSTEVHKFIEELVKIDFPGSKAVSEASSKFGPALVSGPVFFVFEKVSTFI 130
[Close]
BLAST of HO664306 vs. TrEMBL
Match: C6SXW7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.523e-21
Identity = 53/64 (82.81%), Postives = 58/64 (90.62%), Query Frame = 1 
Query:  271 QSLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            +SLVKE  EAGLKKN+TEV KF+EELVKIDFPGSKA SEASSKFGPAL SG +F+VFEKVSTFI
Sbjct:   67 KSLVKEPKEAGLKKNSTEVQKFLEELVKIDFPGSKAASEASSKFGPALASGSVFFVFEKVSTFI 130
[Close]
BLAST of HO664306 vs. TrEMBL
Match: C6TBS7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 9.871e-21
Identity = 52/64 (81.25%), Postives = 57/64 (89.06%), Query Frame = 1 
Query:  271 QSLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            +SLVKE  EAGLKKN+T V KF+EELVKIDFPGSKA SEASSKFGPAL SG +F+VFEKVSTFI
Sbjct:   67 KSLVKEPKEAGLKKNSTGVQKFLEELVKIDFPGSKAASEASSKFGPALASGSVFFVFEKVSTFI 130
[Close]
BLAST of HO664306 vs. TrEMBL
Match: B9GMZ5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_829398 PE=4 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 3.751e-20
Identity = 49/64 (76.56%), Postives = 57/64 (89.06%), Query Frame = 1 
Query:  271 QSLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            ++LVK+  EAGLKK TT V KF++ELVKI+FPGSK VSEASSKFGPA +SGPIF+VFEKVSTFI
Sbjct:   68 KTLVKDPKEAGLKKQTTSVQKFLDELVKIEFPGSKVVSEASSKFGPAYVSGPIFFVFEKVSTFI 131
[Close]
BLAST of HO664306 vs. TrEMBL
Match: B9GYR5_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_414421 PE=4 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 9.239e-19
Identity = 46/64 (71.88%), Postives = 56/64 (87.50%), Query Frame = 1 
Query:  271 QSLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            ++LVK+  EAGLKK +T V KF++ELVKI+FPGSK VSE SSK+GPA +SGPIF+VFEKVSTFI
Sbjct:   67 KTLVKDPKEAGLKKQSTSVQKFLDELVKIEFPGSKLVSETSSKYGPAYVSGPIFFVFEKVSTFI 130
[Close]
BLAST of HO664306 vs. TrEMBL
Match: B9T394_RICCO (Endomembrane-associated protein, putative OS=Ricinus communis GN=RCOM_0096990 PE=4 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 4.585e-18
Identity = 44/64 (68.75%), Postives = 56/64 (87.50%), Query Frame = 1 
Query:  271 QSLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            ++LVKE  +AGLKK++  V KF+EEL KI+FPGSK VSEASSKFGPA +SGP++++FEKVSTFI
Sbjct:   67 KTLVKEPKDAGLKKHSALVQKFLEELAKIEFPGSKPVSEASSKFGPAYVSGPVYFIFEKVSTFI 130
[Close]
BLAST of HO664306 vs. TrEMBL
Match: Q9M3U5_TOBAC (DREPP2 protein OS=Nicotiana tabacum GN=drepp2 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 3.882e-17
Identity = 43/64 (67.19%), Postives = 56/64 (87.50%), Query Frame = 1 
Query:  271 QSLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            ++LVK    +GLKK++T+V KF++ELVKI+FPGSKAVSEASS FGP+ +SGP+ +VFEKVSTFI
Sbjct:   67 KTLVKGPKVSGLKKHSTQVQKFLDELVKIEFPGSKAVSEASSNFGPSYVSGPVLFVFEKVSTFI 130
[Close]
BLAST of HO664306 vs. TrEMBL
Match: O49911_TOBAC (Plasma membrane polypeptide OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 3.882e-17
Identity = 43/64 (67.19%), Postives = 56/64 (87.50%), Query Frame = 1 
Query:  271 QSLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            ++LVK    +GLKK++T+V KF++ELVKI+FPGSKAVSEASS FGP+ +SGP+ +VFEKVSTFI
Sbjct:   67 KTLVKGPKVSGLKKHSTQVQKFLDELVKIEFPGSKAVSEASSNFGPSYVSGPVLFVFEKVSTFI 130
[Close]
BLAST of HO664306 vs. TrEMBL
Match: D7U195_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_155.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00001340001 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 3.882e-17
Identity = 45/64 (70.31%), Postives = 56/64 (87.50%), Query Frame = 1 
Query:  271 QSLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            ++LVKE  EAGLK N+T V KF++ELVKI+FPGSKAV EASSK+GP L+SGP+ ++FEKVSTFI
Sbjct:   67 KTLVKEPKEAGLK-NSTAVQKFLDELVKIEFPGSKAVCEASSKYGPGLVSGPVLFLFEKVSTFI 129
[Close]
BLAST of HO664306 vs. TAIR peptide
Match: AT4G20260.4 (| Symbols: ATPCAP1, PCAP1 | plasma-membrane associated cation-binding protein 1 | chr4:10941593-10943227 FORWARD LENGTH=226)

HSP 1 Score: 85.1149 bits (209), Expect = 2.802e-17
Identity = 39/64 (60.94%), Postives = 52/64 (81.25%), Query Frame = 1 
Query:  271 QSLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            Q+LV++   AGLKKN+  V K++EELVKI+FPGSKAVSEASS FG   ++GP+ ++FEKVS F+
Sbjct:   68 QALVRDPKVAGLKKNSAAVQKYLEELVKIEFPGSKAVSEASSSFGAGYVAGPVTFIFEKVSVFL 131
[Close]
BLAST of HO664306 vs. TAIR peptide
Match: AT4G20260.6 (| Symbols: PCAP1 | plasma-membrane associated cation-binding protein 1 | chr4:10941593-10943227 FORWARD LENGTH=166)

HSP 1 Score: 83.5741 bits (205), Expect = 8.153e-17
Identity = 38/64 (59.38%), Postives = 52/64 (81.25%), Query Frame = 1 
Query:  271 QSLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            ++LV++   AGLKKN+  V K++EELVKI+FPGSKAVSEASS FG   ++GP+ ++FEKVS F+
Sbjct:   67 KALVRDPKVAGLKKNSAAVQKYLEELVKIEFPGSKAVSEASSSFGAGYVAGPVTFIFEKVSVFL 130
[Close]
BLAST of HO664306 vs. TAIR peptide
Match: AT4G20260.5 (| Symbols: PCAP1 | plasma-membrane associated cation-binding protein 1 | chr4:10941593-10943120 FORWARD LENGTH=167)

HSP 1 Score: 83.5741 bits (205), Expect = 8.153e-17
Identity = 38/64 (59.38%), Postives = 52/64 (81.25%), Query Frame = 1 
Query:  271 QSLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            ++LV++   AGLKKN+  V K++EELVKI+FPGSKAVSEASS FG   ++GP+ ++FEKVS F+
Sbjct:   67 KALVRDPKVAGLKKNSAAVQKYLEELVKIEFPGSKAVSEASSSFGAGYVAGPVTFIFEKVSVFL 130
[Close]
BLAST of HO664306 vs. TAIR peptide
Match: AT4G20260.3 (| Symbols: ATPCAP1, PCAP1 | plasma-membrane associated cation-binding protein 1 | chr4:10941593-10943227 FORWARD LENGTH=225)

HSP 1 Score: 83.5741 bits (205), Expect = 8.153e-17
Identity = 38/64 (59.38%), Postives = 52/64 (81.25%), Query Frame = 1 
Query:  271 QSLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            ++LV++   AGLKKN+  V K++EELVKI+FPGSKAVSEASS FG   ++GP+ ++FEKVS F+
Sbjct:   67 KALVRDPKVAGLKKNSAAVQKYLEELVKIEFPGSKAVSEASSSFGAGYVAGPVTFIFEKVSVFL 130
[Close]
BLAST of HO664306 vs. TAIR peptide
Match: AT4G20260.2 (| Symbols: ATPCAP1, PCAP1 | plasma-membrane associated cation-binding protein 1 | chr4:10941593-10943227 FORWARD LENGTH=225)

HSP 1 Score: 83.5741 bits (205), Expect = 8.153e-17
Identity = 38/64 (59.38%), Postives = 52/64 (81.25%), Query Frame = 1 
Query:  271 QSLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            ++LV++   AGLKKN+  V K++EELVKI+FPGSKAVSEASS FG   ++GP+ ++FEKVS F+
Sbjct:   67 KALVRDPKVAGLKKNSAAVQKYLEELVKIEFPGSKAVSEASSSFGAGYVAGPVTFIFEKVSVFL 130
[Close]
BLAST of HO664306 vs. TAIR peptide
Match: AT4G20260.1 (| Symbols: ATPCAP1, PCAP1 | plasma-membrane associated cation-binding protein 1 | chr4:10941593-10943227 FORWARD LENGTH=225)

HSP 1 Score: 83.5741 bits (205), Expect = 8.153e-17
Identity = 38/64 (59.38%), Postives = 52/64 (81.25%), Query Frame = 1 
Query:  271 QSLVKERDEAGLKKNTTEVNKFIEELVKIDFPGSKAVSEASSKFGPALISGPIFYVFEKVSTFI 462
            ++LV++   AGLKKN+  V K++EELVKI+FPGSKAVSEASS FG   ++GP+ ++FEKVS F+
Sbjct:   67 KALVRDPKVAGLKKNSAAVQKYLEELVKIEFPGSKAVSEASSSFGAGYVAGPVTFIFEKVSVFL 130
The following BLAST results are available for this feature:
BLAST of HO664306 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 2
Match NameE-valueIdentityDescription
SRS1_ORYSJ1.547e-846.15Salt stress root protein RS1 OS=Oryza sativa subsp... [more]
SRS1_ORYSI1.547e-846.15Salt stress root protein RS1 OS=Oryza sativa subsp... [more]
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BLAST of HO664306 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q9SMK5_CICAR4.138e-2798.44Plasma membrane intrinsic polypeptide OS=Cicer ari... [more]
B7FH09_MEDTR5.058e-2589.06Putative uncharacterized protein OS=Medicago trunc... [more]
C6SXW7_SOYBN1.523e-2182.81Putative uncharacterized protein OS=Glycine max PE... [more]
C6TBS7_SOYBN9.871e-2181.25Putative uncharacterized protein OS=Glycine max PE... [more]
B9GMZ5_POPTR3.751e-2076.56Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9GYR5_POPTR9.239e-1971.88Predicted protein (Fragment) OS=Populus trichocarp... [more]
B9T394_RICCO4.585e-1868.75Endomembrane-associated protein, putative OS=Ricin... [more]
Q9M3U5_TOBAC3.882e-1767.19DREPP2 protein OS=Nicotiana tabacum GN=drepp2 PE=4... [more]
O49911_TOBAC3.882e-1767.19Plasma membrane polypeptide OS=Nicotiana tabacum P... [more]
D7U195_VITVI3.882e-1770.31Whole genome shotgun sequence of line PN40024, sca... [more]
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BLAST of HO664306 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 6
Match NameE-valueIdentityDescription
AT4G20260.42.802e-1760.94| Symbols: ATPCAP1, PCAP1 | plasma-membrane associ... [more]
AT4G20260.68.153e-1759.38| Symbols: PCAP1 | plasma-membrane associated cati... [more]
AT4G20260.58.153e-1759.38| Symbols: PCAP1 | plasma-membrane associated cati... [more]
AT4G20260.38.153e-1759.38| Symbols: ATPCAP1, PCAP1 | plasma-membrane associ... [more]
AT4G20260.28.153e-1759.38| Symbols: ATPCAP1, PCAP1 | plasma-membrane associ... [more]
AT4G20260.18.153e-1759.38| Symbols: ATPCAP1, PCAP1 | plasma-membrane associ... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008469DREPP plasma membrane polypeptidePFAMPF05558DREPPcoord: 4..101
score: 8.6
NoneNo IPR availableSEGsegsegcoord: 68..95
score

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>HO664306 ID=HO664306; Name=HO664306; organism=Cicer arietinum; type=EST; length=570bp
GCGTGGTCGCGGCCGAGGTACTTGTTGTATATGAGGCTTTATCAACTCAA
ATCAAGGTAAGAAATTATCACACACTCCCTCTATAATAAAATATAAATAA
AAATAGTATTTGTATCTTTACTTCAATTTTTCATATACTTTTATTTCTTA
TATTTCATTCCGGAAAAAATATACATATATACCTAAGTATACACATATGC
ATACATAGATAAGAAAAAAAAATACATATATTGATAGGTAAGGCTATTGA
TAAAATTTTAATCATGTTTTCAGAGTTTGGTCAAAGAACGTGATGAAGCA
GGGCTAAAGAAGAACACAACAGAAGTTAACAAATTCATAGAAGAACTTGT
TAAAATTGATTTTCCTGGATCAAAAGCAGTATCTGAGGCATCTTCAAAGT
TTGGACCAGCCTTAATTTCAGGTCCAATTTTCTATGTTTTTGAGAAAGTA
TCAACTTTCATAGTTACAGAAGAGAAAGTTGAAGAAACAACTCCTACTAC
AGAAACAAAAACAGAAGAGGAAACAAGTGGTGTCAAAGAAAAGGAAATAG
TGATTGAAGAAACGTAAAAA
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: INTERPRO
TermDefinition
IPR008469DREPP