GR404557

EST Overview


Name	GR404557
Unique Name	GR404557
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	566

Libraries


Library Name	Type
LIBEST_024785 ICCV2 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR404557 vs. SwissProt
Match: TYW1_ARATH (tRNA wybutosine-synthesizing protein 1 homolog OS=Arabidopsis thaliana GN=TYW1 PE=2 SV=1)

HSP 1 Score: 333.569 bits (854), Expect = 4.213e-91
Identity = 161/188 (85.64%), Postives = 169/188 (89.89%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            E+L EG  PRHCALSLVGEPIMYPEINALVDELH RRISTFLVTNAQFPEKI  +KPITQLYVSVDA TK+SLKAIDRPLF DFWERFID L   +EK QRTVY LTLVKGWNTE++DAYF LFSIG+PDFIEIKGVTYCGS+ATSKLTMENVPWH DVKAFSEAL LKS GEYEVACEH HSCCVLL
Sbjct:  385 EKLQEGLNPRHCALSLVGEPIMYPEINALVDELHGRRISTFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKALQEKQQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVPWHTDVKAFSEALSLKSNGEYEVACEHAHSCCVLL 572

[Close]

BLAST of GR404557 vs. SwissProt
Match: TYW1_ORYSJ (tRNA wybutosine-synthesizing protein 1 homolog OS=Oryza sativa subsp. japonica GN=TYW1 PE=2 SV=1)

HSP 1 Score: 320.087 bits (819), Expect = 4.820e-87
Identity = 151/188 (80.32%), Postives = 166/188 (88.30%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            ERL EG +PRHCALSLVGEPIMYPEIN L+DELH+R ISTFLVTNAQFP+KIK+LKPITQLYVSVDA TK+SLKA+DRPLF DFWERF+D L    +K QRTVY LTLVKGWN E++D Y KL S+G+PDFIEIKGVTYCGS+ATSKLTMENVPWH+DVK FSEAL LKS G YEVACEH HSCCVLL
Sbjct:  393 ERLAEGLSPRHCALSLVGEPIMYPEINVLIDELHRRHISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHDKDQRTVYRLTLVKGWNAEEIDGYAKLLSLGQPDFIEIKGVTYCGSSATSKLTMENVPWHSDVKDFSEALALKSGGVYEVACEHAHSCCVLL 580

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BLAST of GR404557 vs. SwissProt
Match: TYW1_DANRE (tRNA wybutosine-synthesizing protein 1 homolog OS=Danio rerio GN=tyw1 PE=2 SV=1)

HSP 1 Score: 253.062 bits (645), Expect = 7.236e-67
Identity = 121/188 (64.36%), Postives = 146/188 (77.66%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            ER  EG T +HCALSLVGEPIMYPEIN+ +  LH++ IS+FLVTNAQFPE+I+SL P+TQLYVSVDA TKDSLK IDRPLF DFW+RF+D L    EK QRTVY LTLVK WN +++ AY  L ++G+PDFIE+KGVTYCG ++ S LTM NVPWH +V  F + L      +YE+ACEH HS C+LL
Sbjct:  467 ERFEEGLTVKHCALSLVGEPIMYPEINSFLKLLHQQNISSFLVTNAQFPEEIRSLVPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLRALGEKQQRTVYRLTLVKAWNVDELKAYADLIALGQPDFIEVKGVTYCGESSASSLTMANVPWHEEVIYFVQQL-ANLLPDYEIACEHEHSNCLLL 653

[Close]

BLAST of GR404557 vs. SwissProt
Match: TYW1_XENLA (tRNA wybutosine-synthesizing protein 1 homolog OS=Xenopus laevis GN=tyw1 PE=2 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 2.105e-66
Identity = 122/188 (64.89%), Postives = 144/188 (76.60%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            ER  EG   +HCALSLVGEPIMYPEIN  +  LH + IS+FLVTNAQFPE+I+SL+P+TQLYVSVDA TKDSLK IDRPLF DFW+RFID L    EK QRTVY LTLVK WN +++ AY  L ++G PDFIE+KGVTYCG ++ S LTM NVPWH +V  F E L +    +YEVACEH HS C+L+
Sbjct:  472 ERFEEGLAVKHCALSLVGEPIMYPEINTFLRLLHNQHISSFLVTNAQFPEEIRSLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFIDSLKALAEKQQRTVYRLTLVKAWNVDELKAYADLVALGLPDFIEVKGVTYCGESSASNLTMANVPWHEEVIRFVEEL-VDLLPDYEVACEHEHSNCMLI 658

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BLAST of GR404557 vs. SwissProt
Match: TYW1_HUMAN (tRNA wybutosine-synthesizing protein 1 homolog OS=Homo sapiens GN=TYW1 PE=1 SV=2)

HSP 1 Score: 246.514 bits (628), Expect = 6.772e-65
Identity = 118/188 (62.77%), Postives = 144/188 (76.60%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            ER  EG T +HCALSLVGEPIMYPEIN  +  LH+ +IS+FLVTNAQFP +I++L+P+TQLYVSVDA TKDSLK IDRPLF DFW+RF+D L     K QRTVY LTLVK WN +++ AY +L S+G PDFIE+KGVTYCG ++ S LTM +VPWH +V  F   L +    EYE+ACEH HS C+L+
Sbjct:  469 ERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMAHVPWHEEVVQFVHEL-VDLIPEYEIACEHEHSNCLLI 655

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BLAST of GR404557 vs. SwissProt
Match: TYW1_MOUSE (tRNA wybutosine-synthesizing protein 1 homolog OS=Mus musculus GN=Tyw1 PE=1 SV=1)

HSP 1 Score: 246.128 bits (627), Expect = 8.845e-65
Identity = 119/188 (63.30%), Postives = 141/188 (75.00%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            ER  EG   +HCALSLVGEPIMYPEIN L+  LH+  IS+FLVTNAQFPE+I+ L P+TQLYVSVDA T+D LK IDRPLF DFW+RF+D L     K QRTVY LTLVK WN +++ AY +L S+G PDFIE+KGVTYCG +A S LTM NVPWH +V  F   L +    +YEVACEH HS C+L+
Sbjct:  458 ERFEEGMEVKHCALSLVGEPIMYPEINRLLKLLHQHGISSFLVTNAQFPEEIRKLTPVTQLYVSVDASTRDGLKKIDRPLFKDFWQRFLDSLKALSAKQQRTVYRLTLVKCWNVDELQAYAELVSLGNPDFIEVKGVTYCGESAASSLTMANVPWHEEVVRFVREL-VDLLPDYEVACEHEHSNCLLI 644

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BLAST of GR404557 vs. SwissProt
Match: TYW1_PONAB (tRNA wybutosine-synthesizing protein 1 homolog OS=Pongo abelii GN=TYW1 PE=2 SV=1)

HSP 1 Score: 243.047 bits (619), Expect = 7.488e-64
Identity = 117/188 (62.23%), Postives = 143/188 (76.06%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            ER  EG T +HCALSLVGEPIMYPEIN  +  LH+ +IS+FLVTNAQFP +I++L+P+TQLYVSVDA TKDSLK IDRPLF DFW RF+D L     K QRTVY LTLVK WN +++ AY +L S+G PDFIE+KGVTYCG ++ S LTM +VPWH +V  F   L +    +YE+ACEH HS C+L+
Sbjct:  469 ERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWRRFLDSLKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMAHVPWHEEVVQFVCEL-VDLIPDYEIACEHEHSNCLLI 655

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BLAST of GR404557 vs. SwissProt
Match: TYW1B_HUMAN (tRNA wybutosine-synthesizing protein 1 homolog B OS=Homo sapiens GN=TYW1B PE=2 SV=2)

HSP 1 Score: 239.58 bits (610), Expect = 8.279e-63
Identity = 115/188 (61.17%), Postives = 142/188 (75.53%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            ER  EG T +HCALSLVGEPIMYPEIN  +  LH+ +IS+FLVTNAQFP +I++L+P+TQLYVSVDA TKDSLK IDRPLF DFW++F+D L     K QRTVY L LVK WN +++ AY +L S+G PDFIE+KGVTYC  ++ S LTM +VPWH +V  F   L +    EYE+ACEH HS C+L+
Sbjct:  405 ERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQQFLDSLKALAVKQQRTVYRLMLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCRESSASSLTMAHVPWHEEVVQFVREL-VDLIPEYEIACEHEHSNCLLI 591

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BLAST of GR404557 vs. SwissProt
Match: TYW1_SCHPO (tRNA wybutosine-synthesizing protein 1 OS=Schizosaccharomyces pombe GN=tyw1 PE=2 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 1.198e-53
Identity = 106/195 (54.36%), Postives = 136/195 (69.74%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALF------LKSQG-EYEVACEHVHSCCVLL 566
            +R  E    RHCALSLVGEPI YP IN  V  LH+R IS+FLVTNAQ PE ++++  +TQLYVSVDA TK SLK++DRPLF DFWER +  L + +EK QRTVY +TLVKG+N E +  Y +L  +G P FIE+KGVTY G++  S LTM+NVP++ +V  F + L       L+  G  YE+A EH HSC +L+
Sbjct:  410 DRYEEASRVRHCALSLVGEPIFYPYINEFVSMLHEREISSFLVTNAQHPEALRNMGMVTQLYVSVDASTKQSLKSVDRPLFKDFWERMLTCLEILREKRQRTVYRMTLVKGFNMEQIKEYTELIRLGVPCFIEVKGVTYSGNSDQSPLTMKNVPYYEEVIDFVKKLIEYIDIHLQDLGVRYEIAAEHAHSCSILV 604

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BLAST of GR404557 vs. SwissProt
Match: TYW1_YEAST (tRNA wybutosine-synthesizing protein 1 OS=Saccharomyces cerevisiae GN=TYW1 PE=1 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 9.825e-48
Identity = 98/196 (50.00%), Postives = 134/196 (68.37%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFK--EKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGST--ATSKLTMENVPWHAD----VKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            ER  + F  RHCALSLVGEPI+YP IN  +  LH++ I++FLV NAQ PE ++++  +TQLYVS+DA TK  LK +DRPL+ DFWER ++ L + K  + HQRTV+ LTLVKG+N  DV AY  L   G P FIE+KG T+ GS+    + LTM+N+P++ +    VKAF+  L  +    Y++A EH HS C+L+
Sbjct:  532 ERFAKAFEVRHCALSLVGEPILYPHINKFIQLLHQKGITSFLVCNAQHPEALRNIVKVTQLYVSIDAPTKTELKKVDRPLYKDFWERMVECLEILKTVQNHQRTVFRLTLVKGFNMGDVSAYADLVQRGLPGFIEVKGATFSGSSDGNGNPLTMQNIPFYEECVKFVKAFTTELQRRGL-HYDLAAEHAHSNCLLI 726

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BLAST of GR404557 vs. TrEMBL
Match: A5AHU2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_038533 PE=4 SV=1)

HSP 1 Score: 345.51 bits (885), Expect = 1.443e-93
Identity = 162/188 (86.17%), Postives = 177/188 (94.15%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            ERL+EG +PRHCALSLVGEPIMYPEIN+L++ELH+RRISTFLVTNAQFPEKIK LKP+TQLYVSVDA TKDSLKAIDRPLFGDFWERF+D L   +EK QRTVY LTLVKGWNTE++DAYF LFSIG+PDF+EIKGVTYCGS+ATSKLTMENVPWHADVKAFSEAL LKS+GEYEVACEHVHSCCVLL
Sbjct: 1503 ERLSEGLSPRHCALSLVGEPIMYPEINSLIEELHRRRISTFLVTNAQFPEKIKELKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEEIDAYFNLFSIGKPDFVEIKGVTYCGSSATSKLTMENVPWHADVKAFSEALALKSKGEYEVACEHVHSCCVLL 1690

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BLAST of GR404557 vs. TrEMBL
Match: B9R7T9_RICCO (Cytochrome P450, putative OS=Ricinus communis GN=RCOM_1594210 PE=4 SV=1)

HSP 1 Score: 344.354 bits (882), Expect = 3.214e-93
Identity = 165/188 (87.77%), Postives = 175/188 (93.09%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            ERL EG +PRHCALSLVGEPIMYPEIN LV+ELH+RRISTFLVTNAQFPEKIK LKP+TQLYVSVDA TK+SLKAIDRPLFGDFWERFID L   +EK QRTVY LTLVKGWNTEDVDAYF LFSIG+PDFIEIKGVTYCGS+ATSKLTMENVPWH+DVKAFSEAL LKS+GEYEVACEHVHSCCVLL
Sbjct:  373 ERLNEGLSPRHCALSLVGEPIMYPEINTLVNELHQRRISTFLVTNAQFPEKIKMLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALREKQQRTVYRLTLVKGWNTEDVDAYFNLFSIGQPDFIEIKGVTYCGSSATSKLTMENVPWHSDVKAFSEALALKSKGEYEVACEHVHSCCVLL 560

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BLAST of GR404557 vs. TrEMBL
Match: B9GRY5_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_412724 PE=4 SV=1)

HSP 1 Score: 340.502 bits (872), Expect = 4.641e-92
Identity = 162/188 (86.17%), Postives = 174/188 (92.55%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            ERL EG +PRHCALSLVGEPIMYPEIN LVDELH+R+ISTFLVTNAQFPEKI  LKP+TQLYVSVDA TK+SLKAIDRPLFGDFWERFID L   KEK QRTVY LTLVKGWNTED+DAYF LFS+G+PDFIEIKGVTYCGS+ATSKLTMENVPWH+DVKAFSEA+ LKS+GEYEVACEHVHSCCVLL
Sbjct:  338 ERLNEGLSPRHCALSLVGEPIMYPEINTLVDELHRRQISTFLVTNAQFPEKILMLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKSLKEKQQRTVYRLTLVKGWNTEDLDAYFNLFSVGQPDFIEIKGVTYCGSSATSKLTMENVPWHSDVKAFSEAMALKSKGEYEVACEHVHSCCVLL 525

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BLAST of GR404557 vs. TrEMBL
Match: D7KSL5_ARALY (Flavodoxin family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_895387 PE=4 SV=1)

HSP 1 Score: 335.109 bits (858), Expect = 1.950e-90
Identity = 162/188 (86.17%), Postives = 170/188 (90.43%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            E+L EG  PRHCALSLVGEPIMYPEINALVDELH RRISTFLVTNAQFPEKI  +KPITQLYVSVDA TK+SLKAIDRPLF DFWERFID L   +EK QRTVY LTLVKGWNTE++DAYF LFSIG+PDFIEIKGVTYCGS+ATSKLTMENVPWHADVKAFSEAL LKS GEYEVACEH HSCCVLL
Sbjct:  385 EKLQEGLNPRHCALSLVGEPIMYPEINALVDELHGRRISTFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKALQEKQQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVPWHADVKAFSEALSLKSNGEYEVACEHAHSCCVLL 572

[Close]

BLAST of GR404557 vs. TrEMBL
Match: A3AN61_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_12772 PE=4 SV=1)

HSP 1 Score: 320.087 bits (819), Expect = 6.490e-86
Identity = 151/188 (80.32%), Postives = 166/188 (88.30%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            ERL EG +PRHCALSLVGEPIMYPEIN L+DELH+R ISTFLVTNAQFP+KIK+LKPITQLYVSVDA TK+SLKA+DRPLF DFWERF+D L    +K QRTVY LTLVKGWN E++D Y KL S+G+PDFIEIKGVTYCGS+ATSKLTMENVPWH+DVK FSEAL LKS G YEVACEH HSCCVLL
Sbjct:  376 ERLAEGLSPRHCALSLVGEPIMYPEINVLIDELHRRHISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHDKDQRTVYRLTLVKGWNAEEIDGYAKLLSLGQPDFIEIKGVTYCGSSATSKLTMENVPWHSDVKDFSEALALKSGGVYEVACEHAHSCCVLL 563

[Close]

BLAST of GR404557 vs. TrEMBL
Match: A2XMF8_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_13711 PE=4 SV=1)

HSP 1 Score: 320.087 bits (819), Expect = 6.490e-86
Identity = 151/188 (80.32%), Postives = 166/188 (88.30%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            ERL EG +PRHCALSLVGEPIMYPEIN L+DELH+R ISTFLVTNAQFP+KIK+LKPITQLYVSVDA TK+SLKA+DRPLF DFWERF+D L    +K QRTVY LTLVKGWN E++D Y KL S+G+PDFIEIKGVTYCGS+ATSKLTMENVPWH+DVK FSEAL LKS G YEVACEH HSCCVLL
Sbjct:  393 ERLAEGLSPRHCALSLVGEPIMYPEINVLIDELHRRHISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHDKDQRTVYRLTLVKGWNAEEIDGYAKLLSLGQPDFIEIKGVTYCGSSATSKLTMENVPWHSDVKDFSEALALKSGGVYEVACEHAHSCCVLL 580

[Close]

BLAST of GR404557 vs. TrEMBL
Match: C5WZ57_SORBI (Putative uncharacterized protein Sb01g006590 OS=Sorghum bicolor GN=Sb01g006590 PE=4 SV=1)

HSP 1 Score: 319.316 bits (817), Expect = 1.107e-85
Identity = 152/188 (80.85%), Postives = 165/188 (87.77%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            E+L EG +PRHCALSLVGEPIMYPEINALVDELH+R ISTFLVTNAQFPEKIK+LKPITQLYVSVDA TK+SLKA+DRPLF DFWERF+D L    EK QRTVY LTLVKGWN E++DAY KL  +G+PDFIEIKGVTYCGS+ATSKLTMENVPWH+DVK FSE L  KS G YEVACEH HSCCVLL
Sbjct:  390 EKLEEGLSPRHCALSLVGEPIMYPEINALVDELHRRHISTFLVTNAQFPEKIKALKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHEKDQRTVYRLTLVKGWNAEEIDAYAKLLDLGQPDFIEIKGVTYCGSSATSKLTMENVPWHSDVKEFSEVLASKSGGVYEVACEHAHSCCVLL 577

[Close]

BLAST of GR404557 vs. TrEMBL
Match: A9T8M7_PHYPA (Predicted protein (Fragment) OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_141934 PE=4 SV=1)

HSP 1 Score: 306.22 bits (783), Expect = 9.699e-82
Identity = 148/189 (78.31%), Postives = 163/189 (86.24%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQG-EYEVACEHVHSCCVLL 566
            ERL EG  PRHCALSLVGEPIMYPEIN LV+ELHKRRISTFLVTNAQFPE+I +L+PITQLYVSVDA TK+ LKAIDRPLF DFWERFID L   ++K QRTVY LTLVKGWNTE+V AY +L  +G+PDFIEIKGVTYCGSTATS LTMENVPWH DVKAF+EA+  +  G EYE+ACEH HSCCVLL
Sbjct:  360 ERLAEGMNPRHCALSLVGEPIMYPEINTLVNELHKRRISTFLVTNAQFPERITNLEPITQLYVSVDAATKEKLKAIDRPLFSDFWERFIDSLKALEDKGQRTVYRLTLVKGWNTEEVKAYAELLDLGQPDFIEIKGVTYCGSTATSSLTMENVPWHEDVKAFAEAIAAERGGAEYELACEHSHSCCVLL 548

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BLAST of GR404557 vs. TrEMBL
Match: B4FXB4_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 283.493 bits (724), Expect = 6.732e-75
Identity = 135/167 (80.84%), Postives = 146/167 (87.43%), Query Frame = -1

Query:    3 MYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 503
            MYPEINALVDELH+R ISTFLVTNAQFPEKIK+LKPITQLYVSVDA TK+SLKA+DRPLF DFWERF+D L    EK QRTVY LTLVKGWN E++DAY KL  +G+PDFIEIKGVTYCGS+ATSKLTMENVPWH+DVK FSE L  KS G YEVACEH HSCCVLL
Sbjct:    1 MYPEINALVDELHRRHISTFLVTNAQFPEKIKALKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHEKDQRTVYRLTLVKGWNAEEIDAYAKLLDLGQPDFIEIKGVTYCGSSATSKLTMENVPWHSDVKEFSEVLASKSGGVYEVACEHAHSCCVLL 167

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BLAST of GR404557 vs. TrEMBL
Match: A8IUI9_CHLRE (ADPH--cytochrome P450 reductase-related protein OS=Chlamydomonas reinhardtii GN=NCPR1 PE=4 SV=1)

HSP 1 Score: 268.085 bits (684), Expect = 2.927e-70
Identity = 128/188 (68.09%), Postives = 145/188 (77.13%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            E +  G  PRHCALSLVGEPIMYPEINALV  LH R +STFLVTNAQFP++I+ L P+TQLYVSVDA T  SLKA+DRPLF D+WERF   LT  ++K QRTVY LTLVKGWN E+V  Y +L  +G PDFIEIKGVTYCGS   S LTM NVP+HADV AF EA+     GEY +ACEH HSCC+LL
Sbjct:  256 EAVAAGLAPRHCALSLVGEPIMYPEINALVGRLHARGLSTFLVTNAQFPDRIQQLSPVTQLYVSVDAATPASLKAVDRPLFADYWERFTACLTALRDKRQRTVYRLTLVKGWNMEEVADYARLVDLGRPDFIEIKGVTYCGSGGASSLTMANVPYHADVVAFGEAICQARGGEYGLACEHAHSCCILL 443

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BLAST of GR404557 vs. TAIR peptide
Match: AT1G75200.1 (| Symbols: | flavodoxin family protein / radical SAM domain-containing protein | chr1:28220849-28223597 REVERSE LENGTH=647)

HSP 1 Score: 333.569 bits (854), Expect = 4.490e-92
Identity = 161/188 (85.64%), Postives = 169/188 (89.89%), Query Frame = -1

Query:    3 ERLTEGFTPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQFPEKIKSLKPITQLYVSVDAGTKDSLKAIDRPLFGDFWERFIDPLTVFKEKHQRTVYPLTLVKGWNTEDVDAYFKLFSIGEPDFIEIKGVTYCGSTATSKLTMENVPWHADVKAFSEALFLKSQGEYEVACEHVHSCCVLL 566
            E+L EG  PRHCALSLVGEPIMYPEINALVDELH RRISTFLVTNAQFPEKI  +KPITQLYVSVDA TK+SLKAIDRPLF DFWERFID L   +EK QRTVY LTLVKGWNTE++DAYF LFSIG+PDFIEIKGVTYCGS+ATSKLTMENVPWH DVKAFSEAL LKS GEYEVACEH HSCCVLL
Sbjct:  385 EKLQEGLNPRHCALSLVGEPIMYPEINALVDELHGRRISTFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKALQEKQQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVPWHTDVKAFSEALSLKSNGEYEVACEHAHSCCVLL 572

The following BLAST results are available for this feature:

BLAST of GR404557 vs. SwissProt
BLAST of GR404557 vs. TrEMBL
BLAST of GR404557 vs. TAIR peptide

BLAST of GR404557 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
TYW1_ARATH	4.213e-91	85.64	tRNA wybutosine-synthesizing protein 1 homolog OS=...	[more]
TYW1_ORYSJ	4.820e-87	80.32	tRNA wybutosine-synthesizing protein 1 homolog OS=...	[more]
TYW1_DANRE	7.236e-67	64.36	tRNA wybutosine-synthesizing protein 1 homolog OS=...	[more]
TYW1_XENLA	2.105e-66	64.89	tRNA wybutosine-synthesizing protein 1 homolog OS=...	[more]
TYW1_HUMAN	6.772e-65	62.77	tRNA wybutosine-synthesizing protein 1 homolog OS=...	[more]
TYW1_MOUSE	8.845e-65	63.30	tRNA wybutosine-synthesizing protein 1 homolog OS=...	[more]
TYW1_PONAB	7.488e-64	62.23	tRNA wybutosine-synthesizing protein 1 homolog OS=...	[more]
TYW1B_HUMAN	8.279e-63	61.17	tRNA wybutosine-synthesizing protein 1 homolog B O...	[more]
TYW1_SCHPO	1.198e-53	54.36	tRNA wybutosine-synthesizing protein 1 OS=Schizosa...	[more]
TYW1_YEAST	9.825e-48	50.00	tRNA wybutosine-synthesizing protein 1 OS=Saccharo...	[more]

BLAST of GR404557 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A5AHU2_VITVI	1.443e-93	86.17	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9R7T9_RICCO	3.214e-93	87.77	Cytochrome P450, putative OS=Ricinus communis GN=R...	[more]
B9GRY5_POPTR	4.641e-92	86.17	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
D7KSL5_ARALY	1.950e-90	86.17	Flavodoxin family protein OS=Arabidopsis lyrata su...	[more]
A3AN61_ORYSJ	6.490e-86	80.32	Putative uncharacterized protein OS=Oryza sativa s...	[more]
A2XMF8_ORYSI	6.490e-86	80.32	Putative uncharacterized protein OS=Oryza sativa s...	[more]
C5WZ57_SORBI	1.107e-85	80.85	Putative uncharacterized protein Sb01g006590 OS=So...	[more]
A9T8M7_PHYPA	9.699e-82	78.31	Predicted protein (Fragment) OS=Physcomitrella pat...	[more]
B4FXB4_MAIZE	6.732e-75	80.84	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
A8IUI9_CHLRE	2.927e-70	68.09	ADPH--cytochrome P450 reductase-related protein OS...	[more]

BLAST of GR404557 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G75200.1	4.490e-92	85.64	\| Symbols: \| flavodoxin family protein / radical ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007197	Radical SAM	PFAM	PF04055	Radical_SAM	coord: 13..123 score: 2.8
IPR013917	Wyosine base formation	PFAM	PF08608	Wyosine_form	coord: 127..189 score: 3.9
None	No IPR available	GENE3D	3.80.30.10	no description	coord: 18..143 score: 1.2
None	No IPR available	PANTHER	PTHR13930	RSAFD1-RELATED	coord: 1..189 score: 1.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR404557 ID=GR404557; Name=GR404557; organism=Cicer arietinum; type=EST; length=566bp
GCTAAGAGGACACAGCACGAGTGAACATGCTCACAAGCAACTTCATACTC
CCCTTGGCTTTTCAGAAACAGAGCCTCTGAAAATGCTTTCACGTCGGCAT
GCCAAGGGACATTTTCCATTGTCAATTTTGATGTAGCAGTCGAGCCACAA
TATGTAACTCCCTTGATTTCAATAAAATCAGGTTCTCCTATACTAAAGAG
CTTAAAATAAGCATCGACATCTTCAGTATTCCATCCTTTTACAAGGGTCA
GGGGGTACACAGTACGCTGATGTTTTTCCTTAAAAACAGTCAAGGGATCA
ATAAATCGTTCCCAGAAATCTCCAAAGAGCGGTCTATCGATTGCCTTTAA
AGAGTCCTTTGTTCCGGCATCTACACTGACATATAACTGGGTGATAGGCT
TCAGCGACTTAATTTTCTCTGGAAATTGTGCATTTGTAACTAGAAAAGTG
GAAATCCTCCTTTTGTGCAACTCATCCACAAGTGCATTTATCTCTGGATA
CATAATAGGTTCACCAACAAGAGATAGTGCACAATGTCGTGGTGTGAAGC
CTTCAGTTAATCGCTC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0051536	iron-sulfur cluster binding

Vocabulary: **INTERPRO**
Term	Definition
IPR007197	rSAM
IPR013917	tRNA_wybutosine-synth

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