ES560334

EST Overview


Name	ES560334
Unique Name	ES560334
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	566

Libraries


Library Name	Type
LIBEST_021248 Ascochyta rabiei induced library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of ES560334 vs. SwissProt
Match: FABI_BRANA (Enoyl-[acyl-carrier-protein] reductase [NADH], chloroplastic OS=Brassica napus PE=1 SV=2)

HSP 1 Score: 260.381 bits (664), Expect = 5.979e-73
Identity = 132/178 (74.16%), Postives = 146/178 (82.02%), Query Frame = 2

Query:    2 PADIKANKRYAGSSKWTVQEVAESVKEDFGSIDILVHSLANGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFL 535
            P D+KANKRYAGSS WTVQE AE V++DFGSIDILVHSLANGPEV+KPLLETSRKG                 HFLPI+NPGG+S+SLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRK+ +RVNTISAGPL SRAAKAIGFID MI+YS  NAP+QK L+A+  G  + FL
Sbjct:  172 PEDVKANKRYAGSSNWTVQEAAECVRQDFGSIDILVHSLANGPEVSKPLLETSRKGYLAAISASSYSFVSLLSHFLPIMNPGGASISLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKQNIRVNTISAGPLGSRAAKAIGFIDTMIEYSYNNAPIQKTLTADEVGNAAAFL 349

HSP 2 Score: 35.039 bits (79), Expect = 5.979e-73
Identity = 17/18 (94.44%), Postives = 17/18 (94.44%), Query Frame = 3

Query:  513 VGNAAAFLVSPLASXITG 566
            VGNAAAFLVSPLAS ITG
Sbjct:  342 VGNAAAFLVSPLASAITG 359

[Close]

BLAST of ES560334 vs. SwissProt
Match: FABI_HELPY (Enoyl-[acyl-carrier-protein] reductase [NADH] OS=Helicobacter pylori GN=fabI PE=1 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.979e-20
Identity = 63/167 (37.72%), Postives = 90/167 (53.89%), Query Frame = 2

Query:   71 SVKEDFGSIDILVHSLANGPE--VTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLSVTFSIGYHG 565
            SVK+D GS+D +VHS+A  P+  +   LLETS+                      P+LN G S L+L+Y+ S + +  Y   M  AKAALES  R LA + G K  +RVN +SAGP+R+ A+  I    M++ ++  NAPL+K +S E  G    +L  + S G  G
Sbjct:   77 SVKKDLGSLDFIVHSVAFAPKEALEGSLLETSKSAFNTAMEISVYSLIELTNTLKPLLNNGASVLTLSYLGSTKYMAHYNV-MGLAKAALESAVRYLAVDLG-KHHIRVNALSAGPIRTLASSGIADFRMILKWNEINAPLRKNVSLEEVGNAGMYLLSSLSSGVSG 241

[Close]

BLAST of ES560334 vs. SwissProt
Match: FABI_HELPJ (Enoyl-[acyl-carrier-protein] reductase [NADH] OS=Helicobacter pylori J99 GN=fabI PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 6.636e-20
Identity = 61/167 (36.53%), Postives = 90/167 (53.89%), Query Frame = 2

Query:   71 SVKEDFGSIDILVHSLANGPE--VTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLSVTFSIGYHG 565
            ++K+D GS+D +VHS+A  P+  +   LLETS+                      P+LN G S L+L+Y+ S + +  Y   M  AKAALES  R LA + G K  +RVN +SAGP+R+ A+  I    M++ ++  NAPL+K +S E  G    +L  + S G  G
Sbjct:   77 NIKQDLGSLDFIVHSVAFAPKEALEGSLLETSKSAFNTAMEISVYSLIELTNTLKPLLNNGASVLTLSYLGSTKYMAHYNV-MGLAKAALESAVRYLAVDLG-KHNIRVNALSAGPIRTLASSGIADFRMILKWNEINAPLRKNVSLEEVGNAGMYLLSSLSNGVSG 241

[Close]

BLAST of ES560334 vs. SwissProt
Match: FABI1_RHIME (Enoyl-[acyl-carrier-protein] reductase [NADH] 1 OS=Rhizobium meliloti GN=fabI1 PE=3 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.293e-19
Identity = 61/174 (35.06%), Postives = 94/174 (54.02%), Query Frame = 2

Query:   50 TVQEVAESVKEDFGSIDILVHSLA--NGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLSVTFSIGYHG 565
            T+  V  +++E +G ID +VH++A  +  E+T   L+TSR                       ++N GGS L+LTY  +E+++P Y   M  AKAALE+  R LA + G  R +RVN ISAGP+++ AA  IG    ++ ++  NAPL++ +S E  G  + +L    S G  G
Sbjct:   74 TIDAVFSALEEKWGKIDFVVHAIAFSDKDELTGRYLDTSRDNFARTMDISVYSFTAVAARADRVMNDGGSILTLTYYGAEKVMPHYNV-MGVAKAALEASVRYLAVDLGN-RGIRVNAISAGPIKTLAASGIGDFRYILKWNEYNAPLKRTVSIEEVGNSALYLLSDLSSGVTG 245

[Close]

BLAST of ES560334 vs. SwissProt
Match: FABI_ANASP (Enoyl-[acyl-carrier-protein] reductase [NADH] OS=Anabaena sp. (strain PCC 7120) GN=fabI PE=3 SV=2)

HSP 1 Score: 89.3521 bits (220), Expect = 1.384e-17
Identity = 57/173 (32.95%), Postives = 91/173 (52.60%), Query Frame = 2

Query:   53 VQEVAESVKEDFGSIDILVHSLA--NGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLSVTFSIGYHG 565
            +Q   +++++ +G +DIL+H LA  N  ++T    +TSR G                    P++  GGS ++L+Y+   R +P Y   M  AKA LE+  R LA E G  + +RVN ISAGP+R+ A+ A+G I  MI +    APL++ ++    G  + FL+   + G  G
Sbjct:   74 IQSTFDTIRDKWGRLDILIHCLAFANRDDLTGDFSQTSRAGFATALDISTFSLVQLSGAAKPLMTEGGSIITLSYLGGVRAVPNYNV-MGVAKAGLEASVRYLASELG-SQNIRVNAISAGPIRTLASSAVGGILDMIHHVEQVAPLRRTVTQLEVGNTAAFLASDLASGITG 244

[Close]

BLAST of ES560334 vs. SwissProt
Match: FABI_RICFE (Enoyl-[acyl-carrier-protein] reductase [NADH] OS=Rickettsia felis GN=fabI PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 3.408e-16
Identity = 53/174 (30.46%), Postives = 91/174 (52.30%), Query Frame = 2

Query:   50 TVQEVAESVKEDFGSIDILVHSLA--NGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLSVTFSIGYHG 565
            ++  + + +KE +GS D L+H +A  +  E+    ++TS +                 +    +++ GGS ++LTY  +E++IP Y   M  +KAALE+  + LA + G +  +RVN ISAGP+++ A+ AIG    M+    A APL++  + E  G  + +L    S G  G
Sbjct:   72 SISNLFDDIKEKWGSFDFLLHGMAFADKNELKGRYVDTSLENFHNSLHISCYSLLELSRSAEALMHDGGSIVTLTYYGAEKVIPNYNV-MGISKAALEASVKYLATDLG-ENNIRVNAISAGPIKTLASSAIGDFSTMLKSHAATAPLKRNTTQEDVGGAAVYLFSNLSKGVTG 243

[Close]

BLAST of ES560334 vs. SwissProt
Match: FABI_RICTY (Enoyl-[acyl-carrier-protein] reductase [NADH] OS=Rickettsia typhi GN=fabI PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 4.451e-16
Identity = 53/174 (30.46%), Postives = 90/174 (51.72%), Query Frame = 2

Query:   50 TVQEVAESVKEDFGSIDILVHSLA--NGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLSVTFSIGYHG 565
            ++  +   +KE + S D L+H +A  N  E+    ++TS +                 +    +++ GGS L+LTY  +E++IP Y   M  AKAALE+  + LA + G +  +RVN ISAGP+++ A+ AI     M+ +  + APL++ ++ E  G  + +L    S G  G
Sbjct:   72 SISNLFNDIKEKWNSFDFLLHGMAFANKNELKGRYVDTSLENFYNSLHISCYSLLELSRSAETLMHDGGSILTLTYYGAEKVIPNYNI-MGVAKAALEASVKYLANDMG-ENNIRVNAISAGPIKTLASSAISDFSTMLKFHASTAPLKRNITQEDVGGAAVYLFSELSKGVTG 243

[Close]

BLAST of ES560334 vs. SwissProt
Match: FABI_RICCN (Enoyl-[acyl-carrier-protein] reductase [NADH] OS=Rickettsia conorii GN=fabI PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 4.451e-16
Identity = 54/174 (31.03%), Postives = 91/174 (52.30%), Query Frame = 2

Query:   50 TVQEVAESVKEDFGSIDILVHSLA--NGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLSVTFSIGYHG 565
            ++  + + +KE +GS D L+H +A  +  E+    ++TS +                 +    +++ GGS ++LTY  +E++IP Y   M  AKAALE+  + LA + G +  +RVN ISAGP+++ A+ AIG    M+    A APL++  + E  G  + +L    S G  G
Sbjct:   72 SISNLFDDIKEKWGSFDFLLHGMAFADKNELKGRYVDTSLENFHNSLHISCYSLLELSRSAEALMHNGGSIVTLTYYGAEKVIPNYNV-MGVAKAALEASIKYLANDMG-ENNIRVNAISAGPIKTLASSAIGDFSTMLKSHAATAPLKRNTTQEDVGGAAVYLFSELSKGVTG 243

[Close]

BLAST of ES560334 vs. SwissProt
Match: FABI2_RHIME (Enoyl-[acyl-carrier-protein] reductase [NADH] 2 OS=Rhizobium meliloti GN=fabI2 PE=3 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.814e-16
Identity = 55/174 (31.61%), Postives = 90/174 (51.72%), Query Frame = 2

Query:   50 TVQEVAESVKEDFGSIDILVHSL--ANGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLSVTFSIGYHG 565
            +V  V +++KE +G +D +VH++  ++  E+     +T+R                  K    +++ GG+ L+LTY  S R++P Y   M  AKAALE+  R LA + G  R +RVN ISAGP+R+ A   I     M+ +   N+PL++ ++ E  G  + +L    S G  G
Sbjct:   71 SVDAVVDAIKERWGKLDFVVHAIGFSDKNELKGLYADTTRDNFSRTMVISCFSFTEIAKRAAELMSEGGTMLTLTYGGSMRVMPNYNV-MGVAKAALEASVRYLAADYG-SRGIRVNAISAGPIRTLAGAGISDARAMLSWQQKNSPLRRTVTIEDVGSSALYLLSDLSRGVTG 242

[Close]

BLAST of ES560334 vs. SwissProt
Match: FABI_RICPR (Enoyl-[acyl-carrier-protein] reductase [NADH] OS=Rickettsia prowazekii GN=fabI PE=3 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 9.916e-16
Identity = 54/174 (31.03%), Postives = 89/174 (51.15%), Query Frame = 2

Query:   50 TVQEVAESVKEDFGSIDILVHSLA--NGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLSVTFSIGYHG 565
            ++  +   +KE + S D L+H +A  N  E+    +ETS +                 +    +++ GGS ++LTY  +E++IP Y   M  AKAALE+  + LA + G +  +RVN ISAGP+++ A+ AI     M+    A APL++ ++ E  G  + +L    S G  G
Sbjct:   72 SISNLFNDIKEKWNSFDFLLHGMAFANKNELKGRYVETSLENFHNSLHISCYSLLELSRSAETLMHNGGSIVTLTYYGAEKVIPNYNI-MGVAKAALEASVKYLANDMG-ENNIRVNAISAGPIKTLASSAISDFSTMLKSHAATAPLKRNITQEDVGGAAVYLFSELSKGVTG 243

[Close]

BLAST of ES560334 vs. TrEMBL
Match: O04946_TOBAC (Enoyl-ACP reductase OS=Nicotiana tabacum GN=enr-T2 PE=4 SV=1)

HSP 1 Score: 285.034 bits (728), Expect = 2.796e-78
Identity = 147/179 (82.12%), Postives = 155/179 (86.59%), Query Frame = 2

Query:    2 PADIKANKRYAGSSKWTVQEVAESVKEDFGSIDILVHSLANGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLS 538
            P DIK+NKRYAGSSKWTV E AESVKEDFGSIDILVHSLANGPEVTKPLLET+RKG                KHFLPI+NPGGSS+SLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRK+KVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSA+  G  + FL+
Sbjct:  174 PEDIKSNKRYAGSSKWTVSEAAESVKEDFGSIDILVHSLANGPEVTKPLLETTRKGYLAAISASSYSYVSLLKHFLPIMNPGGSSISLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKKKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSADEVGNTAAFLA 352

HSP 2 Score: 31.9574 bits (71), Expect = 2.796e-78
Identity = 15/18 (83.33%), Postives = 15/18 (83.33%), Query Frame = 3

Query:  513 VGNAAAFLVSPLASXITG 566
            VGN AAFL SPLAS ITG
Sbjct:  344 VGNTAAFLASPLASAITG 361

[Close]

BLAST of ES560334 vs. TrEMBL
Match: C6TKG3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 285.034 bits (728), Expect = 2.796e-78
Identity = 149/179 (83.24%), Postives = 154/179 (86.03%), Query Frame = 2

Query:    2 PADIKANKRYAGSSKWTVQEVAESVKEDFGSIDILVHSLANGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLS 538
            P DIKANKRYAGSSKWTVQEV ESVKEDF SIDILVHSLANGPEVTKPLLETSRKG                KHFLPILNPGGSS+SLTYIASERIIPG+GGGMSSAKAALESDTRVLAFEAGRKRK+RVNTISAGPLRSRAAKAIGFIDMMIDYS ANAPLQKELSAE  G  + FL+
Sbjct:  174 PEDIKANKRYAGSSKWTVQEVVESVKEDFDSIDILVHSLANGPEVTKPLLETSRKGYLAAISASSYSYVSLLKHFLPILNPGGSSISLTYIASERIIPGHGGGMSSAKAALESDTRVLAFEAGRKRKIRVNTISAGPLRSRAAKAIGFIDMMIDYSSANAPLQKELSAEEVGNTAAFLA 352

HSP 2 Score: 31.9574 bits (71), Expect = 2.796e-78
Identity = 15/18 (83.33%), Postives = 15/18 (83.33%), Query Frame = 3

Query:  513 VGNAAAFLVSPLASXITG 566
            VGN AAFL SPLAS ITG
Sbjct:  344 VGNTAAFLASPLASAITG 361

[Close]

BLAST of ES560334 vs. TrEMBL
Match: B5LAU4_CAPAN (Putative enoyl-acyl-carrier-protein reductase OS=Capsicum annuum PE=2 SV=1)

HSP 1 Score: 283.493 bits (724), Expect = 8.092e-78
Identity = 145/179 (81.01%), Postives = 155/179 (86.59%), Query Frame = 2

Query:    2 PADIKANKRYAGSSKWTVQEVAESVKEDFGSIDILVHSLANGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLS 538
            P DIK NKRYAGSSKWTV EVAESVKEDFGSIDILVHSLANGPEVTKPLLET+R+G                KHFLPI+NPGGSS+SLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRK+KVRVNT+SAGPLRSRAAKAIGFIDMMIDYS+ANAPLQKELSA+  G  + FL+
Sbjct:  174 PEDIKTNKRYAGSSKWTVSEVAESVKEDFGSIDILVHSLANGPEVTKPLLETTRRGYLAAISASSYSYVSLLKHFLPIMNPGGSSISLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKQKVRVNTVSAGPLRSRAAKAIGFIDMMIDYSLANAPLQKELSADEVGNTAAFLA 352

HSP 2 Score: 31.9574 bits (71), Expect = 8.092e-78
Identity = 15/18 (83.33%), Postives = 15/18 (83.33%), Query Frame = 3

Query:  513 VGNAAAFLVSPLASXITG 566
            VGN AAFL SPLAS ITG
Sbjct:  344 VGNTAAFLASPLASAITG 361

[Close]

BLAST of ES560334 vs. TrEMBL
Match: O04945_TOBAC (Enoyl-ACP reductase OS=Nicotiana tabacum GN=enr-T1 PE=4 SV=1)

HSP 1 Score: 282.722 bits (722), Expect = 1.377e-77
Identity = 149/180 (82.78%), Postives = 156/180 (86.67%), Query Frame = 2

Query:    2 PADIKANKRYAGSSKWTVQEVAESVKEDFGSIDILVHSLANGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAG-PLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLS 538
            P DIKANKRYAGSSKWTV EVAESVKEDFGSIDILVHSLANGPEVTKPLLETSRKG                KHFLPI+NPGGSS+SLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRK+KVRVNTISAG PLRSRAAKAIGFIDMMI+YSIANAPLQKELSA+  G  + FL+
Sbjct:  174 PEDIKANKRYAGSSKWTVSEVAESVKEDFGSIDILVHSLANGPEVTKPLLETSRKGYLAAISASSYSYVSLLKHFLPIMNPGGSSISLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKKKVRVNTISAGSPLRSRAAKAIGFIDMMIEYSIANAPLQKELSADEVGNTAAFLA 353

HSP 2 Score: 31.9574 bits (71), Expect = 1.377e-77
Identity = 15/18 (83.33%), Postives = 15/18 (83.33%), Query Frame = 3

Query:  513 VGNAAAFLVSPLASXITG 566
            VGN AAFL SPLAS ITG
Sbjct:  345 VGNTAAFLASPLASAITG 362

[Close]

BLAST of ES560334 vs. TrEMBL
Match: B9GYV3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_555276 PE=4 SV=1)

HSP 1 Score: 281.182 bits (718), Expect = 1.795e-77
Identity = 143/179 (79.89%), Postives = 155/179 (86.59%), Query Frame = 2

Query:    2 PADIKANKRYAGSSKWTVQEVAESVKEDFGSIDILVHSLANGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLS 538
            P D+KANKRYAGSSKWTVQEVAESVK+DFGSIDILVHSLANGPEV+KPLLETSRKG                KHFLPI+NPGGSS+SLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRK ++RVNTISAGPLRSRAAKAIGFID MI+YS+ANAPLQKELSA+  G  + FL+
Sbjct:  174 PEDVKANKRYAGSSKWTVQEVAESVKQDFGSIDILVHSLANGPEVSKPLLETSRKGYLAAISASSYSYVSLLKHFLPIMNPGGSSISLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKNRIRVNTISAGPLRSRAAKAIGFIDTMIEYSLANAPLQKELSADEVGNAAAFLA 352

HSP 2 Score: 33.113 bits (74), Expect = 1.795e-77
Identity = 15/18 (83.33%), Postives = 16/18 (88.89%), Query Frame = 3

Query:  513 VGNAAAFLVSPLASXITG 566
            VGNAAAFL SPLAS +TG
Sbjct:  344 VGNAAAFLASPLASAVTG 361

[Close]

BLAST of ES560334 vs. TrEMBL
Match: B9GLM6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_639726 PE=4 SV=1)

HSP 1 Score: 280.796 bits (717), Expect = 2.342e-77
Identity = 142/179 (79.33%), Postives = 155/179 (86.59%), Query Frame = 2

Query:    2 PADIKANKRYAGSSKWTVQEVAESVKEDFGSIDILVHSLANGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLS 538
            P D+KANKRYAGSSKWTVQEVAESVK+D+GSIDILVHSLANGPEVTKPLLETSRKG                KHFLP++NPGGSS+SLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRK ++RVNTISAGPLRSRAAKAIGFID MI+YS+ANAPLQKELSA+  G  + FL+
Sbjct:  174 PEDVKANKRYAGSSKWTVQEVAESVKQDYGSIDILVHSLANGPEVTKPLLETSRKGYLAAISASSYSYVSLLKHFLPLMNPGGSSISLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKNRIRVNTISAGPLRSRAAKAIGFIDTMIEYSLANAPLQKELSADEVGNAAAFLA 352

HSP 2 Score: 33.113 bits (74), Expect = 2.342e-77
Identity = 15/18 (83.33%), Postives = 16/18 (88.89%), Query Frame = 3

Query:  513 VGNAAAFLVSPLASXITG 566
            VGNAAAFL SPLAS +TG
Sbjct:  344 VGNAAAFLASPLASAVTG 361

[Close]

BLAST of ES560334 vs. TrEMBL
Match: C6TIV7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 283.493 bits (724), Expect = 6.769e-77
Identity = 148/179 (82.68%), Postives = 153/179 (85.47%), Query Frame = 2

Query:    2 PADIKANKRYAGSSKWTVQEVAESVKEDFGSIDILVHSLANGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLS 538
            P DIK NKRYAGSSKWTVQEVAESVKEDFGSIDILVHSLANGPEVTKPLLETSR G                KHFLPILNPGGSS+SLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRK+RVNTISAGPLRSRAAKAIGFIDMMIDYS ANAPLQKE+ AE  G  + FL+
Sbjct:  243 PEDIKTNKRYAGSSKWTVQEVAESVKEDFGSIDILVHSLANGPEVTKPLLETSRNGYLAAISASSYSYVSLLKHFLPILNPGGSSISLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKIRVNTISAGPLRSRAAKAIGFIDMMIDYSSANAPLQKEVLAEEVGYTAAFLA 421

HSP 2 Score: 28.8758 bits (63), Expect = 6.769e-77
Identity = 14/18 (77.78%), Postives = 14/18 (77.78%), Query Frame = 3

Query:  513 VGNAAAFLVSPLASXITG 566
            VG  AAFL SPLAS ITG
Sbjct:  413 VGYTAAFLASPLASAITG 430

[Close]

BLAST of ES560334 vs. TrEMBL
Match: D7TIF1_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00033509001 PE=4 SV=1)

HSP 1 Score: 276.174 bits (705), Expect = 1.502e-76
Identity = 141/178 (79.21%), Postives = 151/178 (84.83%), Query Frame = 2

Query:    2 PADIKANKRYAGSSKWTVQEVAESVKEDFGSIDILVHSLANGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFL 535
            P D+K NKRYAGSS WTVQEVAESVK+DFGSIDILVHSLANGPEVTKPLLETSRKG                KHFLPI+NPGG+S+SLTYIASERIIPGYGGGMSSAKA+LESDTRVLAFEAGRK K+RVNTISAGPL SRAAKAIGFIDMMI+YS+ANAPLQKEL AE  G  + FL
Sbjct:  254 PEDVKTNKRYAGSSNWTVQEVAESVKQDFGSIDILVHSLANGPEVTKPLLETSRKGYLAAISASSYSFVSLLKHFLPIMNPGGASISLTYIASERIIPGYGGGMSSAKASLESDTRVLAFEAGRKHKIRVNTISAGPLGSRAAKAIGFIDMMINYSLANAPLQKELHAEEVGNAAAFL 431

HSP 2 Score: 35.039 bits (79), Expect = 1.502e-76
Identity = 17/18 (94.44%), Postives = 17/18 (94.44%), Query Frame = 3

Query:  513 VGNAAAFLVSPLASXITG 566
            VGNAAAFLVSPLAS ITG
Sbjct:  424 VGNAAAFLVSPLASAITG 441

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BLAST of ES560334 vs. TrEMBL
Match: D7SLC5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_4.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025358001 PE=4 SV=1)

HSP 1 Score: 276.559 bits (706), Expect = 9.638e-76
Identity = 142/179 (79.33%), Postives = 153/179 (85.47%), Query Frame = 2

Query:    2 PADIKANKRYAGSSKWTVQEVAESVKEDFGSIDILVHSLANGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLS 538
            P D+KANKRYAGSS WTVQEVAESVK+DFGSIDILVHSLANGPEVTKPLLETSRKG                K F+PI+NPGGSS+SLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRK K+RVNTISAGPLRSRAAKAIGFID MI+YS+ANAPLQKELSA+  G  + FL+
Sbjct:  278 PEDVKANKRYAGSSNWTVQEVAESVKQDFGSIDILVHSLANGPEVTKPLLETSRKGYLAAISASSYSYVSLLKCFIPIMNPGGSSISLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKHKIRVNTISAGPLRSRAAKAIGFIDTMIEYSLANAPLQKELSADEVGNTAAFLA 456

HSP 2 Score: 31.9574 bits (71), Expect = 9.638e-76
Identity = 15/18 (83.33%), Postives = 15/18 (83.33%), Query Frame = 3

Query:  513 VGNAAAFLVSPLASXITG 566
            VGN AAFL SPLAS ITG
Sbjct:  448 VGNTAAFLASPLASAITG 465

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BLAST of ES560334 vs. TrEMBL
Match: B8K204_MALDO (Enoyl-ACP reductase OS=Malus domestica PE=2 SV=1)

HSP 1 Score: 272.707 bits (696), Expect = 1.375e-74
Identity = 140/179 (78.21%), Postives = 149/179 (83.24%), Query Frame = 2

Query:    2 PADIKANKRYAGSSKWTVQEVAESVKEDFGSIDILVHSLANGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLS 538
            P +IK NKRYAGSS WTVQE AE VK DFGSID+LVHSLANGPEV KPLLETSRKG                KHFLPI+NPGGSS+SLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRK+ +RVNTISAGPLRSRAAKAIGFIDMMIDYS ANAPL+KELSAE  G  + FL+
Sbjct:  174 PEEIKTNKRYAGSSNWTVQEAAECVKNDFGSIDVLVHSLANGPEVVKPLLETSRKGYLAAISASSYSYVSLLKHFLPIINPGGSSISLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKKGIRVNTISAGPLRSRAAKAIGFIDMMIDYSSANAPLEKELSAEEVGNTAAFLA 352

HSP 2 Score: 31.9574 bits (71), Expect = 1.375e-74
Identity = 15/18 (83.33%), Postives = 15/18 (83.33%), Query Frame = 3

Query:  513 VGNAAAFLVSPLASXITG 566
            VGN AAFL SPLAS ITG
Sbjct:  344 VGNTAAFLASPLASAITG 361

[Close]

BLAST of ES560334 vs. TAIR peptide
Match: AT2G05990.2 (| Symbols: MOD1, ENR1 | NAD(P)-binding Rossmann-fold superfamily protein | chr2:2322876-2324867 FORWARD LENGTH=390)

HSP 1 Score: 257.299 bits (656), Expect = 1.540e-72
Identity = 130/179 (72.63%), Postives = 145/179 (81.01%), Query Frame = 2

Query:    2 PADIKANKRYAGSSKWTVQEVAESVKEDFGSIDILVHSLANGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLS 538
            P D+K NKRYAGSS WTVQE AE VK+DFGSIDILVHSLANGPEV+KPLLETSRKG                +HFLPI+NPGG+S+SLTYIASERIIPGYGGGMSSAKAALESDTRVLA+EAGRK  +RVNTISAGPL SRAAKAIGFID MI+YS  N P+QK L+A+  G  + FL+
Sbjct:  175 PEDVKTNKRYAGSSNWTVQEAAECVKKDFGSIDILVHSLANGPEVSKPLLETSRKGYLAAISASSYSFVSLLRHFLPIMNPGGASISLTYIASERIIPGYGGGMSSAKAALESDTRVLAYEAGRKSNIRVNTISAGPLGSRAAKAIGFIDTMIEYSYNNGPIQKTLTADEVGNAAAFLA 353

HSP 2 Score: 33.4982 bits (75), Expect = 1.540e-72
Identity = 16/18 (88.89%), Postives = 16/18 (88.89%), Query Frame = 3

Query:  513 VGNAAAFLVSPLASXITG 566
            VGNAAAFL SPLAS ITG
Sbjct:  345 VGNAAAFLASPLASAITG 362

[Close]

BLAST of ES560334 vs. TAIR peptide
Match: AT2G05990.1 (| Symbols: MOD1, ENR1 | NAD(P)-binding Rossmann-fold superfamily protein | chr2:2322876-2324867 FORWARD LENGTH=390)

HSP 1 Score: 257.299 bits (656), Expect = 1.540e-72
Identity = 130/179 (72.63%), Postives = 145/179 (81.01%), Query Frame = 2

Query:    2 PADIKANKRYAGSSKWTVQEVAESVKEDFGSIDILVHSLANGPEVTKPLLETSRKGXXXXXXXXXXXXXXXXKHFLPILNPGGSSLSLTYIASERIIPGYGGGMSSAKAALESDTRVLAFEAGRKRKVRVNTISAGPLRSRAAKAIGFIDMMIDYSIANAPLQKELSAEXGGKCSCFLS 538
            P D+K NKRYAGSS WTVQE AE VK+DFGSIDILVHSLANGPEV+KPLLETSRKG                +HFLPI+NPGG+S+SLTYIASERIIPGYGGGMSSAKAALESDTRVLA+EAGRK  +RVNTISAGPL SRAAKAIGFID MI+YS  N P+QK L+A+  G  + FL+
Sbjct:  175 PEDVKTNKRYAGSSNWTVQEAAECVKKDFGSIDILVHSLANGPEVSKPLLETSRKGYLAAISASSYSFVSLLRHFLPIMNPGGASISLTYIASERIIPGYGGGMSSAKAALESDTRVLAYEAGRKSNIRVNTISAGPLGSRAAKAIGFIDTMIEYSYNNGPIQKTLTADEVGNAAAFLA 353

HSP 2 Score: 33.4982 bits (75), Expect = 1.540e-72
Identity = 16/18 (88.89%), Postives = 16/18 (88.89%), Query Frame = 3

Query:  513 VGNAAAFLVSPLASXITG 566
            VGNAAAFL SPLAS ITG
Sbjct:  345 VGNAAAFLASPLASAITG 362

The following BLAST results are available for this feature:

BLAST of ES560334 vs. SwissProt
BLAST of ES560334 vs. TrEMBL
BLAST of ES560334 vs. TAIR peptide

BLAST of ES560334 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
FABI_BRANA	5.979e-73	74.16	Enoyl-[acyl-carrier-protein] reductase [NADH], chl...	[more]
FABI_HELPY	2.979e-20	37.72	Enoyl-[acyl-carrier-protein] reductase [NADH] OS=H...	[more]
FABI_HELPJ	6.636e-20	36.53	Enoyl-[acyl-carrier-protein] reductase [NADH] OS=H...	[more]
FABI1_RHIME	3.293e-19	35.06	Enoyl-[acyl-carrier-protein] reductase [NADH] 1 OS...	[more]
FABI_ANASP	1.384e-17	32.95	Enoyl-[acyl-carrier-protein] reductase [NADH] OS=A...	[more]
FABI_RICFE	3.408e-16	30.46	Enoyl-[acyl-carrier-protein] reductase [NADH] OS=R...	[more]
FABI_RICTY	4.451e-16	30.46	Enoyl-[acyl-carrier-protein] reductase [NADH] OS=R...	[more]
FABI_RICCN	4.451e-16	31.03	Enoyl-[acyl-carrier-protein] reductase [NADH] OS=R...	[more]
FABI2_RHIME	5.814e-16	31.61	Enoyl-[acyl-carrier-protein] reductase [NADH] 2 OS...	[more]
FABI_RICPR	9.916e-16	31.03	Enoyl-[acyl-carrier-protein] reductase [NADH] OS=R...	[more]

BLAST of ES560334 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
O04946_TOBAC	2.796e-78	82.12	Enoyl-ACP reductase OS=Nicotiana tabacum GN=enr-T2...	[more]
C6TKG3_SOYBN	2.796e-78	83.24	Putative uncharacterized protein OS=Glycine max PE...	[more]
B5LAU4_CAPAN	8.092e-78	81.01	Putative enoyl-acyl-carrier-protein reductase OS=C...	[more]
O04945_TOBAC	1.377e-77	82.78	Enoyl-ACP reductase OS=Nicotiana tabacum GN=enr-T1...	[more]
B9GYV3_POPTR	1.795e-77	79.89	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9GLM6_POPTR	2.342e-77	79.33	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
C6TIV7_SOYBN	6.769e-77	82.68	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7TIF1_VITVI	1.502e-76	79.21	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7SLC5_VITVI	9.638e-76	79.33	Whole genome shotgun sequence of line PN40024, sca...	[more]
B8K204_MALDO	1.375e-74	78.21	Enoyl-ACP reductase OS=Malus domestica PE=2 SV=1	[more]

BLAST of ES560334 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT2G05990.2	1.540e-72	72.63	\| Symbols: MOD1, ENR1 \| NAD(P)-binding Rossmann-fo...	[more]
AT2G05990.1	1.540e-72	72.63	\| Symbols: MOD1, ENR1 \| NAD(P)-binding Rossmann-fo...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002198	Short-chain dehydrogenase/reductase SDR	PANTHER	PTHR19410	SHORT-CHAIN DEHYDROGENASES/REDUCTASE FAMILY MEMBER	coord: 13..178 score: 3.4
IPR002347	Glucose/ribitol dehydrogenase	PRINTS	PR00081	GDHRDH	coord: 30..41 score: 5.8e-05coord: 104..123 score: 5.8e-05coord: 126..143 score: 5.8
IPR014358	Enoyl-[acyl-carrier-protein] reductase (NADH)	PANTHER	PTHR19410:SF12	ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE	coord: 13..178 score: 3.4
IPR016040	NAD(P)-binding domain	GENE3D	3.40.50.720	no description	coord: 7..147 score: 6
None	No IPR available	SEG	seg	seg	coord: 57..72 score
None	No IPR available	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 13..179 score: 1.6

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>ES560334 ID=ES560334; Name=ES560334; organism=Cicer arietinum; type=EST; length=566bp
ACCAGCAGATATAAAAGCAAATAAGCGCTATGCTGGATCCAGCAAATGGA
CAGTTCAGGAAGTTGCTGAATCTGTTAAGGAAGACTTTGGCAGCATAGAC
ATCCTTGTGCACTCACTTGCCAATGGACCAGAGGTGACCAAACCACTGTT
GGAGACATCTCGGAAAGGATATCTTGCTGCATTATCTGCATCTAGTTACT
CCTATGTTTCCTTACTCAAGCATTTTCTTCCAATCTTGAACCCAGGTGGA
TCTTCACTCTCTCTCACATACATCGCTTCAGAGAGGATCATTCCTGGATA
CGGTGGCGGCATGAGCTCTGCAAAAGCTGCACTAGAAAGTGATACACGAG
TGCTTGCTTTTGAAGCTGGTAGAAAGCGCAAAGTTAGAGTCAATACTATA
TCTGCTGGTCCGCTAAGAAGTCGTGCTGCTAAAGCAATTGGATTCATTGA
CATGATGATTGATTATTCAATAGCCAATGCACCTCTACAGAAGGAGCTAT
CAGCTGAGGNAGGTGGGAAATGCAGCTGCTTTCTTAGTGTCACCTTTAGC
ATCGGNTATCACGGGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process
GO:0055114	obsolete oxidation-reduction process
GO:0006633	fatty acid biosynthetic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0004318	enoyl-[acyl-carrier-protein] reductase (NADH) activity
GO:0003824	catalytic activity
GO:0005488	binding

Vocabulary: **INTERPRO**
Term	Definition
IPR002198	DH_sc/Rdtase_SDR
IPR002347	SDR_fam
IPR014358	Enoyl-ACP_Rdtase_NADH
IPR016040	NAD(P)-bd_dom

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