GR399533

EST Overview


Name	GR399533
Unique Name	GR399533
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	697

Libraries


Library Name	Type
LIBEST_024783 ICC4958 Slow drought stresses root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR399533 vs. SwissProt
Match: ASPR1_ORYSJ (Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica GN=Os05g0567100 PE=2 SV=2)

HSP 1 Score: 101.679 bits (252), Expect = 4.060e-21
Identity = 50/129 (38.76%), Postives = 71/129 (55.04%), Query Frame = -2

Query:  223 PLCFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 609
            P+C   +M V W+Q QL Q   ++ + N+  Q               C     +P  SF +G K F L PE++ L+ GEG     + GF A+DIPPP+GP  ILG+VF GA+  V D G ++VGFA++A
Sbjct:  381 PMCNACEMAVVWMQNQLAQNKTQDLILNYINQLCDKLPSPMGESSVDCGSLASMPEISFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWILGDVFMGAYHTVFDYGKMRVGFAKSA 509

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BLAST of GR399533 vs. SwissProt
Match: ASPRX_ORYSJ (Aspartic proteinase OS=Oryza sativa subsp. japonica GN=RAP PE=2 SV=2)

HSP 1 Score: 99.7525 bits (247), Expect = 1.543e-20
Identity = 48/128 (37.50%), Postives = 74/128 (57.81%), Query Frame = -2

Query:  223 LCFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 606
            +C   +M V W++ QL++   +E + N+  Q              SC     +PN +F + +K F+LTPEQ+ ++  +G   V + GFMA DIPPP+GP  ILG+VF GA+  V D G  ++GFA++A
Sbjct:  369 MCSVCEMAVVWIENQLRENKTKELILNYANQLCERLPSPNGESTVSCHQISKMPNLAFTIANKTFILTPEQYIVKLEQGGQTVCISGFMAFDIPPPRGPLWILGDVFMGAYHTVFDFGKDRIGFAKSA 496

[Close]

BLAST of GR399533 vs. SwissProt
Match: ASPR_HORVU (Phytepsin OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 3.437e-20
Identity = 48/129 (37.21%), Postives = 70/129 (54.26%), Query Frame = -2

Query:  223 PLCFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 609
            P+C   +M V W+Q QL Q   ++ + ++  Q               C     +P+  F +G K F L PE++ L+ GEG     + GF A+DIPPP+GP  ILG+VF G +  V D G L++GFA+AA
Sbjct:  380 PMCSACEMAVVWMQNQLAQNKTQDLILDYVNQLCNRLPSPMGESAVDCGSLGSMPDIEFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWILGDVFMGPYHTVFDYGKLRIGFAKAA 508

[Close]

BLAST of GR399533 vs. SwissProt
Match: ASPR_CUCPE (Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 1.306e-19
Identity = 50/128 (39.06%), Postives = 68/128 (53.12%), Query Frame = -2

Query:  223 LCFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 606
            +C   +M V W+Q QL+Q   +E + N+  +               C     +P  SF +G K F L PE++ L+ GEG     + GF A DIPPP+GP  ILG+VF G +  V D G L+VG AEAA
Sbjct:  386 MCSVCEMTVVWMQNQLRQNQTKERIINYINELCDRMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIPPPRGPLWILGDVFMGRYHTVFDFGKLRVGSAEAA 513

[Close]

BLAST of GR399533 vs. SwissProt
Match: CYPR1_CYNCA (Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO1 PE=1 SV=2)

HSP 1 Score: 95.9005 bits (237), Expect = 2.228e-19
Identity = 47/120 (39.17%), Postives = 65/120 (54.17%), Query Frame = -2

Query:  223 VFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 582
            V W+Q Q++Q    E + N+  +               C     +PN +F +G K F L+PEQ+ L+ GEG     + GF A+D+ PP GP  ILG+VF G +  V D G L+VGFAEAA
Sbjct:  354 VVWMQNQIRQNETEENIINYVDKLCERLPSPMGESAVDCSSLSSMPNIAFTVGGKTFNLSPEQYVLKVGEGATAQCISGFTAMDVAPPHGPLWILGDVFMGQYHTVFDYGNLRVGFAEAA 473

[Close]

BLAST of GR399533 vs. SwissProt
Match: CARDB_CYNCA (Procardosin-B OS=Cynara cardunculus GN=cardB PE=1 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 1.035e-16
Identity = 44/127 (34.65%), Postives = 66/127 (51.97%), Query Frame = -2

Query:  226 LCFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEA 606
            +C F +M +  +Q ++K+    + + N   +               C     +PN +F +G K F +TPEQ+  + GEG     + GF ALDI  P+GP  ILG++F G +  V D G L+VGFAEA
Sbjct:  379 ICTFCEMALVRMQNEIKRNETEDNIINHVNEVCDQLPTSSAESIVDCNGISSMPNIAFTIGSKLFEVTPEQYIYKVGEGEAATCISGFTALDIMSPQGPIWILGDMFMGPYHTVFDYGKLRVGFAEA 505

[Close]

BLAST of GR399533 vs. SwissProt
Match: CARDA_CYNCA (Procardosin-A OS=Cynara cardunculus GN=cardA PE=1 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 3.011e-16
Identity = 47/128 (36.72%), Postives = 65/128 (50.78%), Query Frame = -2

Query:  223 LCFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 606
            +C F +M V W+Q ++KQ    + + N+  +               C     +PN SF +G K F LTPEQ+ L+ G+G     + GF A+D     GP  ILG+VF   +  V D G L VGFAEAA
Sbjct:  378 MCTFCEMAVVWMQNEIKQSETEDNIINYANELCEHLSTSSEELQVDCNTLSSMPNVSFTIGGKKFGLTPEQYILKVGKGEATQCISGFTAMD-ATLLGPLWILGDVFMRPYHTVFDYGNLLVGFAEAA 504

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BLAST of GR399533 vs. SwissProt
Match: CATD_HUMAN (Cathepsin D OS=Homo sapiens GN=CTSD PE=1 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.233e-11
Identity = 35/82 (42.68%), Postives = 48/82 (58.54%), Query Frame = -2

Query:  223 CKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 468
            C+    +P  +  LG K + L+PE + L+  +    + L GFM +DIPPP GP  ILG+VF G +  V D    +VGFAEAA
Sbjct:  329 CEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGRYYTVFDRDNNRVGFAEAA 410

[Close]

BLAST of GR399533 vs. SwissProt
Match: CATD_BOVIN (Cathepsin D OS=Bos taurus GN=CTSD PE=1 SV=2)

HSP 1 Score: 69.3218 bits (168), Expect = 2.233e-11
Identity = 35/82 (42.68%), Postives = 47/82 (57.32%), Query Frame = -2

Query:  223 CKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 468
            C+    +P  +  LG K + L+PE + L+  +    V L GFM +DIPPP GP  ILG+VF G +  V D    +VG AEAA
Sbjct:  307 CEKVSSLPEVTVKLGGKDYALSPEDYALKVSQAETTVCLSGFMGMDIPPPGGPLWILGDVFIGRYYTVFDRDQNRVGLAEAA 388

[Close]

BLAST of GR399533 vs. SwissProt
Match: CATD_RAT (Cathepsin D OS=Rattus norvegicus GN=Ctsd PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.916e-11
Identity = 34/82 (41.46%), Postives = 49/82 (59.76%), Query Frame = -2

Query:  223 CKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 468
            C+    +P  +F LG + + L PE++ L+  +    + L GFM +DIPPP GP  ILG+VF G +  V D    +VGFA+AA
Sbjct:  324 CEKVSSLPIITFKLGGQNYELHPEKYILKVSQAGKTICLSGFMGMDIPPPSGPLWILGDVFIGCYYTVFDREYNRVGFAKAA 405

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BLAST of GR399533 vs. TrEMBL
Match: Q2HRQ7_MEDTR (Peptidase A1, pepsin OS=Medicago truncatula GN=MtrDRAFT_AC157894g38v2 PE=3 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 1.158e-31
Identity = 73/134 (54.48%), Postives = 86/134 (64.18%), Query Frame = -2

Query:  223 SRHKSPLCFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 624
            S   +PLC   QMLV WVQ QLKQ+  +E VFN+  Q              SC D   +PN SF +G+KPFVLTPEQ+ LRTGEG+  V L GF+A D+PPPKGP  ILG+VF  A+  V D G LQVGFAEAA
Sbjct:  263 SAKDTPLCSSCQMLVLWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDISKMPNISFTIGNKPFVLTPEQYILRTGEGITQVCLSGFIAFDVPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 396

[Close]

BLAST of GR399533 vs. TrEMBL
Match: Q9SXU0_CICAR (Aspartic proteinase (Fragment) OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 1.975e-31
Identity = 73/130 (56.15%), Postives = 84/130 (64.62%), Query Frame = -2

Query:  223 SPLCFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 612
            +PLC   QMLV WVQ QLKQ+  +E VFN+  Q              SC D   +PN SF +GDKPFVLTPEQ+ LRTGEG+  V L  F+A DIPPPKGP  ILG+VF  A+  V D G LQVGFAEAA
Sbjct:   75 TPLCSSCQMLVIWVQNQLKQKATKERVFNYVNQLCESLPSPSGESVISCNDLSRMPNISFTIGDKPFVLTPEQYVLRTGEGITEVCLSAFIAFDIPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEAA 204

[Close]

BLAST of GR399533 vs. TrEMBL
Match: Q948P0_SOYBN (Aspartic proteinase 2 OS=Glycine max GN=soyAP2 PE=2 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 1.416e-29
Identity = 67/129 (51.94%), Postives = 83/129 (64.34%), Query Frame = -2

Query:  226 SPLCFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEA 612
            +PLC   QMLV W+Q QLKQ+  ++ VFN+  Q              SC     +PN +F +G+KPFVLTPEQ+ LRTGEG+  V L GF+A D+PPPKGP  ILG+VF  A+  V D G LQVGFAEA
Sbjct:  379 TPLCSSCQMLVLWIQNQLKQKATKDRVFNYVNQLCESLPSPSGESVISCNSLSKMPNITFTIGNKPFVLTPEQYILRTGEGITEVCLSGFIAFDVPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 507

[Close]

BLAST of GR399533 vs. TrEMBL
Match: Q93XR0_IPOBA (Putative aspartic protease OS=Ipomoea batatas PE=2 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 1.416e-29
Identity = 65/130 (50.00%), Postives = 84/130 (64.62%), Query Frame = -2

Query:  223 SPLCFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 612
            +PLC   +M V W+Q QLKQ+G +E VF +  Q               C     +PN +F++GDK FVLTPEQ+ L+TGEG+  V + GF+ALD+PPP+GP  ILG+VF GA+  V D G LQVGFAEAA
Sbjct:  375 NPLCTTCEMAVIWLQNQLKQKGIKEKVFEYVDQLCEKLPSPDGESVIDCNSISSMPNVTFVIGDKDFVLTPEQYILKTGEGIAAVCVSGFLALDVPPPQGPLWILGDVFMGAYHTVFDYGNLQVGFAEAA 504

[Close]

BLAST of GR399533 vs. TrEMBL
Match: C6TIU2_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 1.416e-29
Identity = 67/129 (51.94%), Postives = 83/129 (64.34%), Query Frame = -2

Query:  226 SPLCFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEA 612
            +PLC   QMLV W+Q QLKQ+  ++ VFN+  Q              SC     +PN +F +G+KPFVLTPEQ+ LRTGEG+  V L GF+A D+PPPKGP  ILG+VF  A+  V D G LQVGFAEA
Sbjct:   15 TPLCSSCQMLVLWIQNQLKQKATKDRVFNYVNQLCESLPSPSGESVISCNSLSKMPNITFTIGNKPFVLTPEQYILRTGEGITEVCLSGFIAFDVPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 143

[Close]

BLAST of GR399533 vs. TrEMBL
Match: C6TDL8_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 3.154e-29
Identity = 66/129 (51.16%), Postives = 83/129 (64.34%), Query Frame = -2

Query:  226 SPLCFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEA 612
            +PLC   QMLV W+Q QLKQ+  ++ VFN+  Q              SC     +PN +F +G+KPFVLTPEQ+ L+TGEG+  V L GF+A D+PPPKGP  ILG+VF  A+  V D G LQVGFAEA
Sbjct:   48 NPLCSSCQMLVLWIQNQLKQKATKDRVFNYVNQLCESLPSPSGESVISCNSLSKMPNITFTIGNKPFVLTPEQYILKTGEGITEVCLSGFIAFDVPPPKGPLWILGDVFMRAYHTVFDYGNLQVGFAEA 176

[Close]

BLAST of GR399533 vs. TrEMBL
Match: Q8H948_SOYBN (Aspartic proteinase 5 (Fragment) OS=Glycine max GN=soyAP5 PE=2 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 1.565e-28
Identity = 65/134 (48.51%), Postives = 83/134 (61.94%), Query Frame = -2

Query:  223 SRHKSPLCFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 624
            S   + LC   QMLV W+Q QLKQ+  +E VFN+  Q               C   +G+PN +F +GDKPF LTPEQ+ L+TGEG+  V L GF+A DIPPP+GP  ILG+VF   +  V D G L+VGFA+AA
Sbjct:   42 STKDTALCTSCQMLVVWIQNQLKQKKTKEIVFNYVNQLCESLPSPNGESVVDCNSIYGLPNITFTVGDKPFTLTPEQYILKTGEGIAEVCLSGFIAFDIPPPRGPLWILGDVFMRVYHTVFDYGNLRVGFAKAA 175

[Close]

BLAST of GR399533 vs. TrEMBL
Match: B9I6C6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_834438 PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 5.035e-27
Identity = 60/127 (47.24%), Postives = 79/127 (62.20%), Query Frame = -2

Query:  223 CFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 603
            C   QMLV WVQ QL+++  +E   N+  +               C     +PN +F +GDKPF LTPEQ+ L+TGEG+  V + GFMALD+PPP+GP  ILG+VF GA+  + D G L+VGFAEAA
Sbjct:  381 CTACQMLVIWVQNQLREKATKETAINYLDKLCESLPSPMGQSSIDCNSISTMPNITFTIGDKPFSLTPEQYILKTGEGIAQVCISGFMALDVPPPRGPLWILGDVFMGAYHTIFDYGNLEVGFAEAA 507

[Close]

BLAST of GR399533 vs. TrEMBL
Match: B1Q478_CAPCH (Putative aspartic protease OS=Capsicum chinense PE=2 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 2.499e-26
Identity = 59/131 (45.04%), Postives = 78/131 (59.54%), Query Frame = -2

Query:  223 KSPLCFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 615
            ++PLC   +M V W+Q QLKQ+G +E V  +  Q               C     +PN +F + DK FVLTPEQ+ L+TGEG+  + + GF A D+PPP+GP  ILG+VF G +  V D G  QVGFAEAA
Sbjct:  376 EAPLCTVCEMAVVWIQNQLKQQGTKERVLEYVDQLCEKLPSPMGESVVDCNSISSLPNITFTIKDKAFVLTPEQYILKTGEGIASICISGFAAFDVPPPRGPLWILGDVFMGPYHTVFDYGNSQVGFAEAA 506

[Close]

BLAST of GR399533 vs. TrEMBL
Match: Q2VCI9_SOLTU (Aspartic protease-like OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 2.115e-25
Identity = 60/131 (45.80%), Postives = 78/131 (59.54%), Query Frame = -2

Query:  223 KSPLCFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 615
            ++PLC   +M V W+Q QLKQ G +E V  +  Q               C     +P+ SF + DK FVLTPEQ+ L+TGEG+  + + GF ALD+PPP+GP  ILG+VF G +  V D G  QVGFAEAA
Sbjct:  376 EAPLCTACEMAVVWMQNQLKQAGTKEKVLEYVNQLCEKIPSPMGESTIDCNSISSMPDISFTIKDKAFVLTPEQYILKTGEGVATICVSGFAALDVPPPRGPLWILGDVFMGPYHTVFDYGKSQVGFAEAA 506

[Close]

BLAST of GR399533 vs. TAIR peptide
Match: AT1G62290.2 (| Symbols: | Saposin-like aspartyl protease family protein | chr1:23010107-23012681 REVERSE LENGTH=513)

HSP 1 Score: 96.6709 bits (239), Expect = 1.314e-20
Identity = 51/126 (40.48%), Postives = 67/126 (53.17%), Query Frame = -2

Query:  226 CFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEA 603
            C   +M V W+Q QL+Q   +E + N+  +               C     +P  SF +G K F L PE++ L+ GEG     + GF ALDIPPP+GP  ILG+VF G +  V D G  QVGFAEA
Sbjct:  387 CPACEMAVVWIQSQLRQNMTQERIVNYINEICERMPSPNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDIPPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

[Close]

BLAST of GR399533 vs. TAIR peptide
Match: AT1G62290.1 (| Symbols: | Saposin-like aspartyl protease family protein | chr1:23010107-23012681 REVERSE LENGTH=513)

HSP 1 Score: 96.6709 bits (239), Expect = 1.314e-20
Identity = 51/126 (40.48%), Postives = 67/126 (53.17%), Query Frame = -2

Query:  226 CFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEA 603
            C   +M V W+Q QL+Q   +E + N+  +               C     +P  SF +G K F L PE++ L+ GEG     + GF ALDIPPP+GP  ILG+VF G +  V D G  QVGFAEA
Sbjct:  387 CPACEMAVVWIQSQLRQNMTQERIVNYINEICERMPSPNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDIPPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

[Close]

BLAST of GR399533 vs. TAIR peptide
Match: AT1G11910.1 (| Symbols: APA1, ATAPA1 | aspartic proteinase A1 | chr1:4017119-4019874 REVERSE LENGTH=506)

HSP 1 Score: 94.7449 bits (234), Expect = 4.993e-20
Identity = 49/127 (38.58%), Postives = 67/127 (52.76%), Query Frame = -2

Query:  223 CFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 603
            C   +M V W+Q QL+Q   +E + N+  +               C     +P  S  +G K F L PE++ L+ GEG     + GF+ALD+ PP+GP  ILG+VF G +  V D G  QVGFAEAA
Sbjct:  380 CSACEMAVVWIQSQLRQNMTQERILNYVNELCERLPSPMGESAVDCAQLSTMPTVSLTIGGKVFDLAPEEYVLKVGEGPVAQCISGFIALDVAPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEAA 506

[Close]

BLAST of GR399533 vs. TAIR peptide
Match: AT4G04460.2 (| Symbols: | Saposin-like aspartyl protease family protein | chr4:2225232-2227746 FORWARD LENGTH=504)

HSP 1 Score: 80.8777 bits (198), Expect = 7.462e-16
Identity = 40/128 (31.25%), Postives = 64/128 (50.00%), Query Frame = -2

Query:  223 LCFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 606
            +C   +M   W++ +L Q   +E +  +  +               C     +P  +F +G + F LTP+ +  + GEG+      GF A+DI PP+GP  ILG++F G +  V D G  +VGFA+AA
Sbjct:  377 MCSACEMAAVWMESELTQNQTQERILAYAAELCDHIPTQNQQSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFTAMDIAPPRGPLWILGDIFMGPYHTVFDYGKGRVGFAKAA 504

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BLAST of GR399533 vs. TAIR peptide
Match: AT4G04460.1 (| Symbols: | Saposin-like aspartyl protease family protein | chr4:2225232-2227746 FORWARD LENGTH=508)

HSP 1 Score: 80.8777 bits (198), Expect = 7.462e-16
Identity = 40/128 (31.25%), Postives = 64/128 (50.00%), Query Frame = -2

Query:  223 LCFFFQMLVFWVQIQLKQRGPREGVFNFGIQXXXXXXXXXXXXXXSCKDFFGVPNFSFLLGDKPFVLTPEQFFLRTGEGLPGVSLRGFMALDIPPPKGPFGILGEVFRGAFPPVLD*GTLQVGFAEAA 606
            +C   +M   W++ +L Q   +E +  +  +               C     +P  +F +G + F LTP+ +  + GEG+      GF A+DI PP+GP  ILG++F G +  V D G  +VGFA+AA
Sbjct:  381 MCSACEMAAVWMESELTQNQTQERILAYAAELCDHIPTQNQQSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFTAMDIAPPRGPLWILGDIFMGPYHTVFDYGKGRVGFAKAA 508

The following BLAST results are available for this feature:

BLAST of GR399533 vs. SwissProt
BLAST of GR399533 vs. TrEMBL
BLAST of GR399533 vs. TAIR peptide

BLAST of GR399533 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ASPR1_ORYSJ	4.060e-21	38.76	Aspartic proteinase oryzasin-1 OS=Oryza sativa sub...	[more]
ASPRX_ORYSJ	1.543e-20	37.50	Aspartic proteinase OS=Oryza sativa subsp. japonic...	[more]
ASPR_HORVU	3.437e-20	37.21	Phytepsin OS=Hordeum vulgare PE=1 SV=1	[more]
ASPR_CUCPE	1.306e-19	39.06	Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1	[more]
CYPR1_CYNCA	2.228e-19	39.17	Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO...	[more]
CARDB_CYNCA	1.035e-16	34.65	Procardosin-B OS=Cynara cardunculus GN=cardB PE=1 ...	[more]
CARDA_CYNCA	3.011e-16	36.72	Procardosin-A OS=Cynara cardunculus GN=cardA PE=1 ...	[more]
CATD_HUMAN	2.233e-11	42.68	Cathepsin D OS=Homo sapiens GN=CTSD PE=1 SV=1	[more]
CATD_BOVIN	2.233e-11	42.68	Cathepsin D OS=Bos taurus GN=CTSD PE=1 SV=2	[more]
CATD_RAT	2.916e-11	41.46	Cathepsin D OS=Rattus norvegicus GN=Ctsd PE=1 SV=1	[more]

BLAST of GR399533 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q2HRQ7_MEDTR	1.158e-31	54.48	Peptidase A1, pepsin OS=Medicago truncatula GN=Mtr...	[more]
Q9SXU0_CICAR	1.975e-31	56.15	Aspartic proteinase (Fragment) OS=Cicer arietinum ...	[more]
Q948P0_SOYBN	1.416e-29	51.94	Aspartic proteinase 2 OS=Glycine max GN=soyAP2 PE=...	[more]
Q93XR0_IPOBA	1.416e-29	50.00	Putative aspartic protease OS=Ipomoea batatas PE=2...	[more]
C6TIU2_SOYBN	1.416e-29	51.94	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6TDL8_SOYBN	3.154e-29	51.16	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q8H948_SOYBN	1.565e-28	48.51	Aspartic proteinase 5 (Fragment) OS=Glycine max GN...	[more]
B9I6C6_POPTR	5.035e-27	47.24	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B1Q478_CAPCH	2.499e-26	45.04	Putative aspartic protease OS=Capsicum chinense PE...	[more]
Q2VCI9_SOLTU	2.115e-25	45.80	Aspartic protease-like OS=Solanum tuberosum PE=2 S...	[more]

BLAST of GR399533 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT1G62290.2	1.314e-20	40.48	\| Symbols: \| Saposin-like aspartyl protease famil...	[more]
AT1G62290.1	1.314e-20	40.48	\| Symbols: \| Saposin-like aspartyl protease famil...	[more]
AT1G11910.1	4.993e-20	38.58	\| Symbols: APA1, ATAPA1 \| aspartic proteinase A1 \|...	[more]
AT4G04460.2	7.462e-16	31.25	\| Symbols: \| Saposin-like aspartyl protease famil...	[more]
AT4G04460.1	7.462e-16	31.25	\| Symbols: \| Saposin-like aspartyl protease famil...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 12..26 score
None	No IPR available	PANTHER	PTHR13683:SF83	ASPARTIC PROTEINASE-RELATED	coord: 51..126 score: 3.9
None	No IPR available	SEG	seg	seg	coord: 42..56 score
None	No IPR available	SEG	seg	seg	coord: 17..32 score: NAcoord: 49..63 score
IPR001461	Peptidase A1	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 51..126 score: 3.9
IPR001461	Peptidase A1	PFAM	PF00026	Asp	coord: 48..119 score: 4.7
IPR009007	Peptidase aspartic, catalytic	GENE3D	2.40.70.10	no description	coord: 49..120 score: 1.1
IPR021109	Peptidase aspartic	SUPERFAMILY	50630	Acid proteases	coord: 50..126 score: 2.2

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR399533 ID=GR399533; Name=GR399533; organism=Cicer arietinum; type=EST; length=697bp
CCCGGAAAAGGACCCAAACTTTTAATTAGGGGAAATTTGGCCCCCTGGAA
CAAACGGGGAAAGAAAAAAAAATACCTACCGAAAACATATATATGGGAAA
ACCTGAAGGGAACCAAAGGGAAAGTAATTACCTAATAGCCCAACCGCTTA
TTTACAATGGAAAAACCAAATTATGAAAAAAATTCCCTAAAAAATTACCC
CCAAAGGGGGATGGGGGAACCAAGCAGCTTCCGCAAAACCAACTTGAAGG
GTTCCCTAATCCAAAACCGGGGGAAATGCCCCCCTGAAAACCTCCCCCAA
AATCCCAAAGGGACCCTTTGGGGGAGGAATATCCAAAGCCATAAACCCCC
TAAGGGAAACCCCCGGAAGGCCCTCCCCCGTTCTTAAAAAAAACTGTTCT
GGGGTGAGGACAAAAGGTTTGTCCCCCAAAAGGAAAGAAAAGTTTGGCAC
CCCAAAAAAATCCTTACAGCTTAACCCTGATTCCCCGGAGGGACTTGGAA
AACCCCCACCCAGTTGAATCCCAAAATTGAAAACCCCTTCCCTGGGCCCC
CTTTGTTTTAATTGAATCTGGACCCAAAAAACAAGCATCTGGAAAAAAAA
ACACAAAGGAGATTTGTGGCGGGAACCCCACCCTGTTTATTTTCATTTAA
AACCCAACCCCCCCCCCCGGGGAAAAACCCCGCTAAACCAAAAAAAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004190	aspartic-type endopeptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **INTERPRO**
Term	Definition
IPR001461	Aspartic_peptidase_A1
IPR009007	Peptidase aspartic, catalytic
IPR021109	Peptidase_aspartic_dom_sf

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