GR914349

EST Overview


Name	GR914349
Unique Name	GR914349
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	395

Libraries


Library Name	Type
LIBEST_024968 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. JG-62) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR914349 vs. SwissProt
Match: NRL4B_TOBAC (Bifunctional nitrilase/nitrile hydratase NIT4B OS=Nicotiana tabacum GN=NIT4B PE=2 SV=1)

HSP 1 Score: 205.682 bits (522), Expect = 6.084e-53
Identity = 97/117 (82.91%), Postives = 111/117 (94.87%), Query Frame = -1

Query:   24 PIINDGPLIAEVDMGSDFNAQTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 374
            P++N+GP+ AEVDMG + +  TVRATVVQASTIFYDTPATLDKAERLLAEAASYG+Q+VVFPEA+IGGYPRGS FGVS+GNRTAKG+E+FRKYH++AIDVPGPEVDRLAAMAGKYKV
Sbjct:    7 PVVNEGPMFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLDKAERLLAEAASYGAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYKV 123

[Close]

BLAST of GR914349 vs. SwissProt
Match: NRL4A_TOBAC (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Nicotiana tabacum GN=NIT4A PE=2 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 1.147e-51
Identity = 97/117 (82.91%), Postives = 109/117 (93.16%), Query Frame = -1

Query:   24 PIINDGPLIAEVDMGSDFNAQTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 374
            P +N+GPL AEVDMG + +  TVRATVVQASTIFYDTPATL KAERLLAEAASYG+Q+VVFPEA+IGGYPRGS FGVS+GNRTAKG+E+FRKYH++AIDVPGPEVDRLAAMAGKYKV
Sbjct:    7 PAVNEGPLFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLVKAERLLAEAASYGAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYKV 123

[Close]

BLAST of GR914349 vs. SwissProt
Match: NRL4_ORYSJ (Bifunctional nitrilase/nitrile hydratase NIT4 OS=Oryza sativa subsp. japonica GN=NIT4 PE=2 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 4.370e-43
Identity = 87/117 (74.36%), Postives = 101/117 (86.32%), Query Frame = -1

Query:   24 PLIAEVDM--GSDFNAQTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFG----VSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 356
            P+IAEV+M  G+D  A TVRATVVQAST+FYDTPATLDKAERL+ EAA YGSQ+VVFPEA++GGYPRGS FG    +S+GN   KG+E+FRKYH+AAI+VPGPEV RLAAMAGKYKV
Sbjct:   13 PVIAEVEMNGGADSGAATVRATVVQASTVFYDTPATLDKAERLIEEAAGYGSQLVVFPEAFVGGYPRGSTFGFGANISIGNPKDKGKEEFRKYHAAAIEVPGPEVTRLAAMAGKYKV 129

[Close]

BLAST of GR914349 vs. SwissProt
Match: NRL4_ARATH (Bifunctional nitrilase/nitrile hydratase NIT4 OS=Arabidopsis thaliana GN=NIT4 PE=1 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 3.699e-42
Identity = 82/117 (70.09%), Postives = 99/117 (84.62%), Query Frame = -1

Query:   24 PIINDGPLIAEVDMGSDFNAQTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 374
            P  N   +  E+DM +  ++  VRATVVQAST+FYDTPATLDKAERLL+EAA  GSQ+VVFPEA+IGGYPRGS F +++G+RTAKGR+DFRKYH++AIDVPGPEV+RLA MA KYKV
Sbjct:   14 PQTNGHQIFPEIDMSAGDSSSIVRATVVQASTVFYDTPATLDKAERLLSEAAENGSQLVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAIDVPGPEVERLALMAKKYKV 130

[Close]

BLAST of GR914349 vs. SwissProt
Match: NRL3_ARATH (Nitrilase 3 OS=Arabidopsis thaliana GN=NIT3 PE=1 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 3.248e-30
Identity = 59/98 (60.20%), Postives = 81/98 (82.65%), Query Frame = -1

Query:   24 AQTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 317
            + TVR T+VQ+ST++ DTPATLDKAE+ + EAAS G+++V+FPEA+IGGYPRG  FG+++G    +GR++FR YH++AI VPGPEV+RLA +AGK  V
Sbjct:   22 SSTVRVTIVQSSTVYNDTPATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPRGFRFGLAVGVHNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNV 119

[Close]

BLAST of GR914349 vs. SwissProt
Match: NRL2_ARATH (Nitrilase 2 OS=Arabidopsis thaliana GN=NIT2 PE=1 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 3.248e-30
Identity = 60/95 (63.16%), Postives = 79/95 (83.16%), Query Frame = -1

Query:   24 VRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 308
            VRAT+VQAST++ DTPATL+KA + + EAAS GS++VVFPEA+IGGYPRG  FG+ +G    +GR++FRKYH++AI VPGPEV++LA +AGK  V
Sbjct:   18 VRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAFIGGYPRGFRFGLGVGVHNEEGRDEFRKYHASAIKVPGPEVEKLAELAGKNNV 112

[Close]

BLAST of GR914349 vs. SwissProt
Match: NRL1_ARATH (Nitrilase 1 OS=Arabidopsis thaliana GN=NIT1 PE=1 SV=2)

HSP 1 Score: 123.635 bits (309), Expect = 3.040e-28
Identity = 57/96 (59.38%), Postives = 78/96 (81.25%), Query Frame = -1

Query:   24 TVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 311
            TVR T+VQ+ST++ DTPAT+DKAE+ + EAAS G+++V+FPE +IGGYPRG  FG+++G    +GR++FRKYH++AI VPGPEV RLA +A K  V
Sbjct:   24 TVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGVHNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHV 119

[Close]

BLAST of GR914349 vs. SwissProt
Match: NIT1_YEAST (Putative nitrilase-like protein NIT1 OS=Saccharomyces cerevisiae GN=NIT1 PE=5 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 3.734e-10
Identity = 34/80 (42.50%), Postives = 49/80 (61.25%), Query Frame = -1

Query:   39 TPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPG----PEVDRLAAMA 266
            T  TL K      E    G+++VV PEA +GGYP+GS FGV LG R  +GRE++ KY + AI++      PE+ +L A++
Sbjct:   18 TKDTLKKILSYEKEIKESGAKLVVIPEATLGGYPKGSNFGVYLGYRLQEGREEYAKYLAEAIEIGNGEKYPEISQLCALS 97

[Close]

BLAST of GR914349 vs. SwissProt
Match: NRLB_KLEPO (Nitrilase, bromoxynil-specific OS=Klebsiella pneumoniae subsp. ozaenae GN=bxn PE=1 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 6.369e-10
Identity = 34/96 (35.42%), Postives = 52/96 (54.17%), Query Frame = -1

Query:   24 TVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 311
            T +A  VQA  ++ D  AT DK   L+A+AA+ G+Q+V FPE +I GYP     G  L +   +      KY   AI   GPE++++   A ++ +
Sbjct:    4 TFKAAAVQAEPVWMDAAATADKTVTLVAKAAAAGAQLVAFPELWIPGYP-----GFMLTHNQTETLPFIIKYRKQAIAADGPEIEKIRCAAQEHNI 94

[Close]

BLAST of GR914349 vs. SwissProt
Match: NRL_BACSX (Nitrilase OS=Bacillus sp. (strain OxB-1) GN=nit PE=1 SV=2)

HSP 1 Score: 59.3066 bits (142), Expect = 7.042e-9
Identity = 36/99 (36.36%), Postives = 58/99 (58.59%), Query Frame = -1

Query:   24 NAQTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 320
            N    R   VQAS +  D  AT+DK  RL+ EAA+ G++++ FPEA+I GYP    + + LGN    G + + + +  ++++P   V +L++ AG  KV
Sbjct:    3 NYPKYRVAAVQASPVLLDLDATIDKTCRLVDEAAANGAKVIAFPEAFIPGYP----WWIWLGN-ADYGMKYYIQLYKNSVEIPSLAVQKLSS-AGTNKV 95

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BLAST of GR914349 vs. TrEMBL
Match: C6T972_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 215.312 bits (547), Expect = 1.564e-54
Identity = 104/117 (88.89%), Postives = 111/117 (94.87%), Query Frame = -1

Query:   24 PIINDGPLIAEVDMGSDFNAQTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 374
            P IN+GPL AEVDMGSDFNA TVRATVVQASTIFYDTPATLDKAERLLAEA SYGSQ+VVFPEA++GGYPRGS FG+S+GNRT KGRE+FRKYHSAAIDVPGPEVDRLAAMAGKYKV
Sbjct:    8 PTINEGPLFAEVDMGSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAEATSYGSQLVVFPEAFVGGYPRGSAFGLSIGNRTVKGREEFRKYHSAAIDVPGPEVDRLAAMAGKYKV 124

[Close]

BLAST of GR914349 vs. TrEMBL
Match: Q5QGZ8_LUPAN (Nitrilase 4A OS=Lupinus angustifolius GN=NIT4A PE=2 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 2.668e-54
Identity = 105/117 (89.74%), Postives = 112/117 (95.73%), Query Frame = -1

Query:   24 PIINDGPLIAEVDMGSDFNAQTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 374
            P +NDGPL AEV+M SDFNA TVRATVVQASTIFYDTPATLDKAERLLAEAASYG+QIVVFPEA+IGGYPRGS FGVS+GNRTAKG+EDFRKYHSAAIDVPGPEVDRLAA+AGKYKV
Sbjct:    7 PTVNDGPLFAEVNMSSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAEAASYGAQIVVFPEAFIGGYPRGSNFGVSIGNRTAKGKEDFRKYHSAAIDVPGPEVDRLAALAGKYKV 123

[Close]

BLAST of GR914349 vs. TrEMBL
Match: Q3LRV4_LUPAN (Nitrilase 4B OS=Lupinus angustifolius GN=NIT4B PE=2 SV=1)

HSP 1 Score: 202.216 bits (513), Expect = 1.370e-50
Identity = 99/117 (84.62%), Postives = 108/117 (92.31%), Query Frame = -1

Query:   24 PIINDGPLIAEVDMGSDFNAQTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 374
            P +ND PL AEVDM S F + TVRATVVQASTIFYDTPATLDKAERLL +AASYG+QIVVFPEA+IGGYPRGS FGVS+GNRTAKG+E+FRKYHSAAIDVPGPEVDRL+AMAGKYKV
Sbjct:    8 PTVNDEPLFAEVDMASYFTSTTVRATVVQASTIFYDTPATLDKAERLLVQAASYGAQIVVFPEAFIGGYPRGSNFGVSIGNRTAKGKEEFRKYHSAAIDVPGPEVDRLSAMAGKYKV 124

[Close]

BLAST of GR914349 vs. TrEMBL
Match: B9SCY8_RICCO (Nitrilase, putative OS=Ricinus communis GN=RCOM_1282320 PE=4 SV=1)

HSP 1 Score: 201.06 bits (510), Expect = 3.052e-50
Identity = 97/121 (80.17%), Postives = 110/121 (90.91%), Query Frame = -1

Query:   24 PGQVPIINDGPLIAEVDMGSDFNAQTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 386
            P   P+ +DGPL AEVDMG+D +A TVRATVVQAST+FYDTPATL KAERLLAEAA YGSQ+VVFPEA+IGGYPRGS FG ++G+RTAKGRE+FRKYH+AAIDVPGPEVD+LAAMAGKYKV
Sbjct:    5 PTPPPLTSDGPLFAEVDMGADASAPTVRATVVQASTVFYDTPATLAKAERLLAEAAGYGSQLVVFPEAFIGGYPRGSTFGAAIGSRTAKGREEFRKYHAAAIDVPGPEVDQLAAMAGKYKV 125

[Close]

BLAST of GR914349 vs. TrEMBL
Match: B9MYU3_POPTR (Nitrilase 1 OS=Populus trichocarpa GN=NIT1 PE=4 SV=1)

HSP 1 Score: 192.586 bits (488), Expect = 1.086e-47
Identity = 93/114 (81.58%), Postives = 105/114 (92.11%), Query Frame = -1

Query:   24 NDGPLIAEVDMGSDFNAQTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 365
            ++ PL AEVDM SD +A TVRATV+QAST+FYDTPATLDKAER LAEAA YGSQ+VVFPEA+IGGYPRGS FG ++G+RTAKGREDFRKYH++AIDVPGPEVDRLAAMAGKYKV
Sbjct:    9 SETPLFAEVDMCSDSSATTVRATVIQASTVFYDTPATLDKAERFLAEAAGYGSQLVVFPEAFIGGYPRGSSFGATIGSRTAKGREDFRKYHASAIDVPGPEVDRLAAMAGKYKV 122

[Close]

BLAST of GR914349 vs. TrEMBL
Match: D7TCQ6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_80.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00036074001 PE=4 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 9.191e-47
Identity = 92/104 (88.46%), Postives = 101/104 (97.12%), Query Frame = -1

Query:   24 MGSDFNAQTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 335
            MG+D +A TVRATVVQAST+FYDTPATLDKAERLLAEA+SYGSQ+VVFPEA+IGGYPRGS FGV++GNRTAKGREDFRKYH+AAIDVPGPEVDRLAAMAGKYKV
Sbjct:    1 MGADSSAPTVRATVVQASTVFYDTPATLDKAERLLAEASSYGSQLVVFPEAFIGGYPRGSNFGVTIGNRTAKGREDFRKYHAAAIDVPGPEVDRLAAMAGKYKV 104

[Close]

BLAST of GR914349 vs. TrEMBL
Match: B9SCY5_RICCO (Nitrilase, putative OS=Ricinus communis GN=RCOM_1282290 PE=4 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 3.996e-42
Identity = 84/104 (80.77%), Postives = 94/104 (90.38%), Query Frame = -1

Query:   24 MGSDFNAQTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 335
            M +D +  TVRATVVQAS++FYDTPATLDKAERLLAEA   GSQ+VVFPEA+IGGYPRGS FG S+GNRT KGRE+FRKYH+AA+DVPGPEVDRLAAMAGKYKV
Sbjct:    1 MEADASTPTVRATVVQASSVFYDTPATLDKAERLLAEATGEGSQLVVFPEAFIGGYPRGSTFGSSIGNRTQKGREEFRKYHAAAVDVPGPEVDRLAAMAGKYKV 104

[Close]

BLAST of GR914349 vs. TrEMBL
Match: B7EVI5_ORYSJ (cDNA clone:001-020-F10, full insert sequence OS=Oryza sativa subsp. japonica PE=2 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 8.902e-42
Identity = 87/117 (74.36%), Postives = 101/117 (86.32%), Query Frame = -1

Query:   24 PLIAEVDM--GSDFNAQTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFG----VSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 356
            P+IAEV+M  G+D  A TVRATVVQAST+FYDTPATLDKAERL+ EAA YGSQ+VVFPEA++GGYPRGS FG    +S+GN   KG+E+FRKYH+AAI+VPGPEV RLAAMAGKYKV
Sbjct:   13 PVIAEVEMNGGADSGAATVRATVVQASTVFYDTPATLDKAERLIEEAAGYGSQLVVFPEAFVGGYPRGSTFGFGANISIGNPKDKGKEEFRKYHAAAIEVPGPEVTRLAAMAGKYKV 129

[Close]

BLAST of GR914349 vs. TrEMBL
Match: Q6H851_ORYSJ (Os02g0635000 protein OS=Oryza sativa subsp. japonica GN=OJ1626_B09.5 PE=2 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 1.518e-41
Identity = 83/115 (72.17%), Postives = 101/115 (87.83%), Query Frame = -1

Query:   24 GPLIAEVDM--GSDFNAQ-TVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 359
            GP++ EV+M  G+D  A  TVRATVVQA T+FYDTPATLDKAE+L+AEAA YGSQIV+FPE ++GGYP GS FG+++GNR+AKG+EDF KYH+AAIDVPGPEV RLAA+AGKYK+
Sbjct:   11 GPVVTEVEMNAGADQGATGTVRATVVQACTVFYDTPATLDKAEKLVAEAAGYGSQIVLFPEVFVGGYPHGSTFGLTIGNRSAKGKEDFHKYHAAAIDVPGPEVTRLAALAGKYKI 125

[Close]

BLAST of GR914349 vs. TrEMBL
Match: A9T599_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_88254 PE=4 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 1.518e-41
Identity = 81/110 (73.64%), Postives = 98/110 (89.09%), Query Frame = -1

Query:   24 LIAEVDMGSDFNAQTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 353
            +IAEV+MG     ++VRATVVQASTIFYDTPATL+KAERL+A+AA+ GSQ+VVFPEA+IGGYPRG+ FGV +G RT KG+EDFRKYH +AIDVPGPE DRLAA+AG+YK+
Sbjct:    8 VIAEVEMGMALGTRSVRATVVQASTIFYDTPATLEKAERLIADAAAQGSQLVVFPEAFIGGYPRGNTFGVVIGTRTPKGKEDFRKYHGSAIDVPGPETDRLAALAGRYKL 117

[Close]

BLAST of GR914349 vs. TAIR peptide
Match: AT5G22300.1 (| Symbols: NIT4, AtNIT4 | nitrilase 4 | chr5:7379401-7381764 FORWARD LENGTH=355)

HSP 1 Score: 169.859 bits (429), Expect = 3.897e-43
Identity = 82/117 (70.09%), Postives = 99/117 (84.62%), Query Frame = -1

Query:   24 PIINDGPLIAEVDMGSDFNAQTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 374
            P  N   +  E+DM +  ++  VRATVVQAST+FYDTPATLDKAERLL+EAA  GSQ+VVFPEA+IGGYPRGS F +++G+RTAKGR+DFRKYH++AIDVPGPEV+RLA MA KYKV
Sbjct:   14 PQTNGHQIFPEIDMSAGDSSSIVRATVVQASTVFYDTPATLDKAERLLSEAAENGSQLVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAIDVPGPEVERLALMAKKYKV 130

[Close]

BLAST of GR914349 vs. TAIR peptide
Match: AT3G44320.1 (| Symbols: NIT3, AtNIT3 | nitrilase 3 | chr3:15993419-15995493 FORWARD LENGTH=346)

HSP 1 Score: 130.183 bits (326), Expect = 3.422e-31
Identity = 59/98 (60.20%), Postives = 81/98 (82.65%), Query Frame = -1

Query:   24 AQTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 317
            + TVR T+VQ+ST++ DTPATLDKAE+ + EAAS G+++V+FPEA+IGGYPRG  FG+++G    +GR++FR YH++AI VPGPEV+RLA +AGK  V
Sbjct:   22 SSTVRVTIVQSSTVYNDTPATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPRGFRFGLAVGVHNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNV 119

[Close]

BLAST of GR914349 vs. TAIR peptide
Match: AT3G44300.1 (| Symbols: NIT2, AtNIT2 | nitrilase 2 | chr3:15983351-15985172 FORWARD LENGTH=339)

HSP 1 Score: 130.183 bits (326), Expect = 3.422e-31
Identity = 60/95 (63.16%), Postives = 79/95 (83.16%), Query Frame = -1

Query:   24 VRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 308
            VRAT+VQAST++ DTPATL+KA + + EAAS GS++VVFPEA+IGGYPRG  FG+ +G    +GR++FRKYH++AI VPGPEV++LA +AGK  V
Sbjct:   18 VRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAFIGGYPRGFRFGLGVGVHNEEGRDEFRKYHASAIKVPGPEVEKLAELAGKNNV 112

[Close]

BLAST of GR914349 vs. TAIR peptide
Match: AT3G44310.3 (| Symbols: NIT1, ATNIT1, NITI | nitrilase 1 | chr3:15986901-15988841 FORWARD LENGTH=346)

HSP 1 Score: 123.635 bits (309), Expect = 3.203e-29
Identity = 57/96 (59.38%), Postives = 78/96 (81.25%), Query Frame = -1

Query:   24 TVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 311
            TVR T+VQ+ST++ DTPAT+DKAE+ + EAAS G+++V+FPE +IGGYPRG  FG+++G    +GR++FRKYH++AI VPGPEV RLA +A K  V
Sbjct:   24 TVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGVHNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHV 119

[Close]

BLAST of GR914349 vs. TAIR peptide
Match: AT3G44310.1 (| Symbols: NIT1, ATNIT1, NITI | nitrilase 1 | chr3:15986901-15988841 FORWARD LENGTH=346)

HSP 1 Score: 123.635 bits (309), Expect = 3.203e-29
Identity = 57/96 (59.38%), Postives = 78/96 (81.25%), Query Frame = -1

Query:   24 TVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 311
            TVR T+VQ+ST++ DTPAT+DKAE+ + EAAS G+++V+FPE +IGGYPRG  FG+++G    +GR++FRKYH++AI VPGPEV RLA +A K  V
Sbjct:   24 TVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGVHNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHV 119

The following BLAST results are available for this feature:

BLAST of GR914349 vs. SwissProt
BLAST of GR914349 vs. TrEMBL
BLAST of GR914349 vs. TAIR peptide

BLAST of GR914349 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
NRL4B_TOBAC	6.084e-53	82.91	Bifunctional nitrilase/nitrile hydratase NIT4B OS=...	[more]
NRL4A_TOBAC	1.147e-51	82.91	Bifunctional nitrilase/nitrile hydratase NIT4A OS=...	[more]
NRL4_ORYSJ	4.370e-43	74.36	Bifunctional nitrilase/nitrile hydratase NIT4 OS=O...	[more]
NRL4_ARATH	3.699e-42	70.09	Bifunctional nitrilase/nitrile hydratase NIT4 OS=A...	[more]
NRL3_ARATH	3.248e-30	60.20	Nitrilase 3 OS=Arabidopsis thaliana GN=NIT3 PE=1 S...	[more]
NRL2_ARATH	3.248e-30	63.16	Nitrilase 2 OS=Arabidopsis thaliana GN=NIT2 PE=1 S...	[more]
NRL1_ARATH	3.040e-28	59.38	Nitrilase 1 OS=Arabidopsis thaliana GN=NIT1 PE=1 S...	[more]
NIT1_YEAST	3.734e-10	42.50	Putative nitrilase-like protein NIT1 OS=Saccharomy...	[more]
NRLB_KLEPO	6.369e-10	35.42	Nitrilase, bromoxynil-specific OS=Klebsiella pneum...	[more]
NRL_BACSX	7.042e-9	36.36	Nitrilase OS=Bacillus sp. (strain OxB-1) GN=nit PE...	[more]

BLAST of GR914349 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6T972_SOYBN	1.564e-54	88.89	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q5QGZ8_LUPAN	2.668e-54	89.74	Nitrilase 4A OS=Lupinus angustifolius GN=NIT4A PE=...	[more]
Q3LRV4_LUPAN	1.370e-50	84.62	Nitrilase 4B OS=Lupinus angustifolius GN=NIT4B PE=...	[more]
B9SCY8_RICCO	3.052e-50	80.17	Nitrilase, putative OS=Ricinus communis GN=RCOM_12...	[more]
B9MYU3_POPTR	1.086e-47	81.58	Nitrilase 1 OS=Populus trichocarpa GN=NIT1 PE=4 SV...	[more]
D7TCQ6_VITVI	9.191e-47	88.46	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9SCY5_RICCO	3.996e-42	80.77	Nitrilase, putative OS=Ricinus communis GN=RCOM_12...	[more]
B7EVI5_ORYSJ	8.902e-42	74.36	cDNA clone:001-020-F10, full insert sequence OS=Or...	[more]
Q6H851_ORYSJ	1.518e-41	72.17	Os02g0635000 protein OS=Oryza sativa subsp. japoni...	[more]
A9T599_PHYPA	1.518e-41	73.64	Predicted protein OS=Physcomitrella patens subsp. ...	[more]

BLAST of GR914349 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT5G22300.1	3.897e-43	70.09	\| Symbols: NIT4, AtNIT4 \| nitrilase 4 \| chr5:73794...	[more]
AT3G44320.1	3.422e-31	60.20	\| Symbols: NIT3, AtNIT3 \| nitrilase 3 \| chr3:15993...	[more]
AT3G44300.1	3.422e-31	63.16	\| Symbols: NIT2, AtNIT2 \| nitrilase 2 \| chr3:15983...	[more]
AT3G44310.3	3.203e-29	59.38	\| Symbols: NIT1, ATNIT1, NITI \| nitrilase 1 \| chr3...	[more]
AT3G44310.1	3.203e-29	59.38	\| Symbols: NIT1, ATNIT1, NITI \| nitrilase 1 \| chr3...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000132	Nitrilase/cyanide hydratase, conserved site	PROSITE	PS00920	NITRIL_CHT_1	coord: 62..77 score
IPR003010	Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase	GENE3D	3.60.110.10	no description	coord: 28..124 score: 1.4
IPR003010	Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase	PFAM	PF00795	CN_hydrolase	coord: 31..124 score: 1.7
IPR003010	Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase	PROFILE	PS50263	CN_HYDROLASE	coord: 30..130 score: 13
IPR003010	Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase	SUPERFAMILY	56317	Carbon-nitrogen hydrolase	coord: 27..124 score: 1.1

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR914349 ID=GR914349; Name=GR914349; organism=Cicer arietinum; type=EST; length=395bp
GATTAGCGTGGTCGCGGCCGAGGTACTTTATACTTCCCTGCCATTGCTGC
CAATCTATCCACCTCAGGACCGGGCACGTCAATGGCAGCTGAATGATACT
TCCTGAAATCTTCTCTACCCTTGGCAGTGCGGTTACCAAGAGAAACTCCA
AAAACAGAACCACGCGGGTAGCCACCAATATATGCTTCTGGAAACACCAC
AATTTGGGATCCATAGCTAGCAGCTTCTGCCAGTAATCTCTCAGCTTTAT
CTAAAGTGGCGGGAGTGTCGTAGAAGATTGTGGAAGCTTGAACAACAGTG
GCGCGGACAGTTTGTGCGTTGAAATCGGAGCCCATATCGACCTCGGCGAT
GAGTGGGCCGTCATTGATGATTGGAACCTGCCCGGGCGGCCGCCC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006807	nitrogen compound metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR000132	Nitrilase/CN_hydratase_CS
IPR003010	C-N_Hydrolase

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