HO062804

EST Overview
NameHO062804
Unique NameHO062804
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length672
Libraries
Library NameType
LIBEST_026409 Chickpea drought stressed cDNA SSH library AB2cdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
InterProScan analysis for Cicer arietinum unigene v12010-12-29
Homology
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BLAST of HO062804 vs. SwissProt
Match: APX1_ORYSJ (L-ascorbate peroxidase 1, cytosolic OS=Oryza sativa subsp. japonica GN=APX1 PE=1 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 8.973e-7
Identity = 29/45 (64.44%), Postives = 30/45 (66.67%), Query Frame = -2 
Query:  207 PSDKALLSDPVFRPLVEKXXXXXXXXXXXXXXAHLKLSELGFAEA 341
            PSDKALLSDP FRPLVEK              AHLKLSELGFA+A
Sbjct:  206 PSDKALLSDPAFRPLVEKYAADEKAFFEDYKEAHLKLSELGFADA 250
[Close]
BLAST of HO062804 vs. TrEMBL
Match: Q9LKG6_ASTPN (Ascorbate peroxidase (Fragment) OS=Astragalus penduliflorus PE=2 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 6.487e-8
Identity = 29/45 (64.44%), Postives = 30/45 (66.67%), Query Frame = -2 
Query:  207 PSDKALLSDPVFRPLVEKXXXXXXXXXXXXXXAHLKLSELGFAEA 341
            PSDKALL+DPVFRPLVEK              AH KLSELGFAEA
Sbjct:   79 PSDKALLTDPVFRPLVEKYAADEDAFFADYAVAHQKLSELGFAEA 123

HSP 2 Score: 28.4906 bits (62), Expect = 6.487e-8
Identity = 21/48 (43.75%), Postives = 31/48 (64.58%), Query Frame = -1 
Query:  430 KGFTH*RECFGQGLGG*ANQEYWWLWSGGSTPIGSCNTKEAFLGFGGP 573
            KG  H R+ FG+G+ G ++Q+   L SGG T IG+ + + +  GFGGP
Sbjct:    9 KGSDHLRDVFGKGM-GLSDQDIVAL-SGGHT-IGAAHKERS--GFGGP 51
The following BLAST results are available for this feature:
BLAST of HO062804 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 1
Match NameE-valueIdentityDescription
APX1_ORYSJ8.973e-764.44L-ascorbate peroxidase 1, cytosolic OS=Oryza sativ... [more]
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BLAST of HO062804 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 1
Match NameE-valueIdentityDescription
Q9LKG6_ASTPN6.487e-864.44Ascorbate peroxidase (Fragment) OS=Astragalus pend... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 1..10
score
NoneNo IPR availableGENE3D1.10.420.10no descriptioncoord: 51..96
score: 3.2
NoneNo IPR availableSEGsegsegcoord: 51..63
score: NAcoord: 82..96
score
IPR010255Haem peroxidaseSUPERFAMILY48113Heme-dependent peroxidasescoord: 55..104
score: 7

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>HO062804 ID=HO062804; Name=HO062804; organism=Cicer arietinum; type=EST; length=672bp
AACGGAAAAAGCTGGGGATTACCCTCTTTAACAATGTTATTTCAATAGAG
GATCAACAAGAAAGAAAACAATCAAACTTCACATTCATCGTGTCTGACAA
AATACATCCAAAAGCAACCAAAATATAAGACCAACGCCTGTGAAATTTAT
GGAAGAATCAGGGAACCCCAGCCTCTCTAAACACCTTACACCAACTATTT
TGCTTAGGCCTCAGCAAACCCTAGCTCGGAGAGCTTAAGATGAGCCTCAG
CGTAATCAGCAAAGAAGGCATCTTCATCCGCAGCATATTTCTCTACAAGA
GGACGGAATACAGGGTCACTCAAAAGTGCCTTATCACTTGGCAACTTAAG
AAGGCCTTCCTTCTCACCATTCAACAACTTCCGTGAAAGTAAAGAGTTGC
CCAAAAATGAAAAGAATGGAAATTCCATGGGGCCCTCCAAATCCCAGAAA
CGCTTCCTTGGTGTTGCAGCTTCCAATGGGTGTGGACCCCCCGGACCAAA
GCCACCAATATTCTTGATTCGCTTAGCCCCCTAAGCCTTGCCCAAAACAT
TCCCTTCAATGGGTAAAACCTTTTGGTGGCATTCAGGCAAGCCAACCCCC
CTGGTGGTTGGCTCAGGGCTGGTCCTCCCCTACCCGGGGTGGGAAAGGGA
ACTTTCAGGGTCCCCAGTGGAC
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004601peroxidase activity
GO:0020037heme binding
Vocabulary: Biological Process
TermDefinition
GO:0006979response to oxidative stress
GO:0055114obsolete oxidation-reduction process
Vocabulary: INTERPRO
TermDefinition
IPR010255Haem_peroxidase_sf