HO063403

EST Overview


Name	HO063403
Unique Name	HO063403
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	529

Libraries


Library Name	Type
LIBEST_026410 Chickpea drought stressed cDNA SSH library AR1	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of HO063403 vs. SwissProt
Match: SYIC_MOUSE (Isoleucyl-tRNA synthetase, cytoplasmic OS=Mus musculus GN=Iars PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 2.352e-42
Identity = 87/180 (48.33%), Postives = 124/180 (68.89%), Query Frame = -3

Query:    6 LFDQATLQKSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVCDGSE------ESIHYCSFPE-EEGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHPDAD 524
            L+++ T+++S N+ D+W+ S  QSL+ F   EM  YRLYTVVP L+KF+D LTN YVR NR+RLKG +G EDC  AL TLF+VLL  C++MAP+TPF TE++YQN++ + D +        SIHY   P   E    ++ E +VSRM ++I+L R IR+R   P+K PL+E+V++H D +
Sbjct:  679 LYNEHTVRESPNITDRWVLSFMQSLLGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGESGVEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKLLIDPASLRDKDTLSIHYLMLPRVREELIDKKTENAVSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPE 858

[Close]

BLAST of HO063403 vs. SwissProt
Match: SYIC_HUMAN (Isoleucyl-tRNA synthetase, cytoplasmic OS=Homo sapiens GN=IARS PE=1 SV=2)

HSP 1 Score: 171.014 bits (432), Expect = 3.072e-42
Identity = 88/180 (48.89%), Postives = 122/180 (67.78%), Query Frame = -3

Query:    6 LFDQATLQKSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMR------KVCDGSEESIHYCSFPE-EEGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHPDAD 524
            L+++ T+++S N+ D+WI S  QSL+ F   EM  YRLYTVVP L+KF+D LTN YVR NR+RLKG  G EDC  AL TLF+VLL  C++MAP+TPF TE++YQN++       V D    SIHY   P   E    ++ E +VS+M ++I+L R IR+R   P+K PL+E+V++H D +
Sbjct:  679 LYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPE 858

[Close]

BLAST of HO063403 vs. SwissProt
Match: SYIC_DICDI (Probable isoleucyl-tRNA synthetase, cytoplasmic OS=Dictyostelium discoideum GN=ileS PE=3 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 1.168e-41
Identity = 81/162 (50.00%), Postives = 114/162 (70.37%), Query Frame = -3

Query:   21 SSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVC--DGSEESIHYCSFPEE-EGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIV 497
            S NV+D+WI ++ QSL+ FVR EM  YRLYTVVP L++F+++LTN +VR NRKRLKG  G+ DC  AL+ L+ VL+  C  M PFTPFFTE +YQN++K    +   +S+HY  FPE  +     RIE+++SRM  +I+L R  R+R  KP+K PL+E +++
Sbjct:  692 SENVMDKWILASCQSLIAFVRAEMAAYRLYTVVPKLVRFIEDLTNWFVRLNRKRLKGSNGDADCLAALNILYEVLMTICIAMGPFTPFFTEYMYQNLKKALPKEKQMDSVHYVMFPEPIQEAFNTRIEEAISRMQVVIELGRAARDRRTKPIKFPLKEFMVI 853

[Close]

BLAST of HO063403 vs. SwissProt
Match: SYIC_CAEEL (Isoleucyl-tRNA synthetase, cytoplasmic OS=Caenorhabditis elegans GN=irs-1 PE=2 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 3.756e-40
Identity = 83/175 (47.43%), Postives = 115/175 (65.71%), Query Frame = -3

Query:    3 LFDQATLQKSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVCDGSEESIHYCSFPE-EEGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHPDADF 524
            +FD      S NV+D+WI S T SLV FVR+EMD YRLY VV  L KF D LTNIY+R NRKR+KG  G  +   AL+ L  VL+L  ++MAPFTPFF E ++ N++KV   +EES+H+   P+ +E    E +E+ V  M  +IDL R +R+R    +K PL+EM++++ D+ F
Sbjct:  678 VFDMNVHVASENVMDRWIESFTNSLVAFVRKEMDSYRLYAVVGPLTKFFDTLTNIYIRLNRKRVKGDNGLHEQHHALAALGRVLILIVRLMAPFTPFFCEYIWLNLKKVIGSTEESVHFLMLPKPDESLIDETVERRVEVMRNVIDLVRLVRDREGLAVKYPLKEMIVINRDSQF 852

[Close]

BLAST of HO063403 vs. SwissProt
Match: SYIC_YEAST (Isoleucyl-tRNA synthetase, cytoplasmic OS=Saccharomyces cerevisiae GN=ILS1 PE=1 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 6.406e-40
Identity = 85/177 (48.02%), Postives = 117/177 (66.10%), Query Frame = -3

Query:    3 KSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVC----------DGSEESIHYCSFP-EEEGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHPDADF 500
            KS NV+D+WI ++ QSLV F+ +EM  Y+LYTVVP LL F+D LTN Y+RFNR+RLKG  G EDC  AL++LF+ L    + MAPFTPF +E +Y  +++            DG   S+H+ S+P  ++    E IE +VSRM ++IDL RNIRE+    LKTPL+ +VI+H D  +
Sbjct:  687 KSDNVMDRWILASMQSLVQFIHEEMGQYKLYTVVPKLLNFIDELTNWYIRFNRRRLKGENGVEDCLKALNSLFDALFTFVRAMAPFTPFLSESIYLRLKEYIPEAVLAKYGKDG--RSVHFLSYPVVKKEYFDEAIETAVSRMQSVIDLGRNIREKKTISLKTPLKTLVILHSDESY 861

[Close]

BLAST of HO063403 vs. SwissProt
Match: SYIC_SCHPO (Isoleucyl-tRNA synthetase, cytoplasmic OS=Schizosaccharomyces pombe GN=SPBC8D2.06 PE=2 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 1.477e-36
Identity = 82/181 (45.30%), Postives = 118/181 (65.19%), Query Frame = -3

Query:    3 VLFDQATLQKSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVC-----DGSEESIHYCSFPEEEGK-GGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHPDADF 527
            V  D ATL  SSNV+D+WI +  QSL+ FV +EM  YRLYTVVP LL  ++ +TN Y+RFNR+RLKG  GE +   AL+ LF VL    ++M PFTPF TE +YQ++R        + S  S+H+  FP  + +   E + + V RM TII+LAR +RE++N  LKTPL+ ++++  + ++
Sbjct:  676 VFDDAATL--SSNVMDRWILARCQSLIGFVDEEMKQYRLYTVVPQLLGLIEEMTNWYIRFNRRRLKGEDGEIETINALNVLFEVLFTLVRIMGPFTPFITENIYQHLRNYMPIDKNEISLRSVHFLPFPTYKSELDDETVLRRVKRMQTIIELARYVREQNNISLKTPLKTLIVILTNEEY 854

[Close]

BLAST of HO063403 vs. SwissProt
Match: SYI_TETTH (Isoleucyl-tRNA synthetase OS=Tetrahymena thermophila GN=ILSA PE=3 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 1.805e-34
Identity = 78/171 (45.61%), Postives = 111/171 (64.91%), Query Frame = -3

Query:    3 KSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVC-DGS---EESIHYCSFP-EEEGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHPDADF 500
            K +N  D+WI  + Q+L+++VR EM+ Y LY VVP L+ FL+NLTN Y+R NR RLKG  G E+  T+L+ LFNV+L S  +M+P  PF TE  YQN+RKV   GS   EESIH+   P  ++    E+IE++  RM  II+ AR +RE+    LK P+  + +++ D +F
Sbjct:  697 KLTNPTDRWIIISCQNLINYVRIEMEKYHLYNVVPRLIHFLENLTNWYIRLNRNRLKGDYGLEEQETSLNVLFNVILNSTILMSPLVPFITESFYQNLRKVIPKGSSYLEESIHFLRIPTPKQELLDEKIERNFERMQNIINFARTLREKRKVSLKQPIMSLTVINQDQEF 867

[Close]

BLAST of HO063403 vs. SwissProt
Match: SYI_BDEBA (Isoleucyl-tRNA synthetase OS=Bdellovibrio bacteriovorus GN=ileS PE=3 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 2.698e-30
Identity = 69/167 (41.32%), Postives = 100/167 (59.88%), Query Frame = -3

Query:    6 QKSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVCDGSEESIHYCSFPEEE-GKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHPDAD 503
            +KS N+LDQW+ S    L+    +EMD YRLY VVP+LL+F+++LTN Y+RFNR         E  R A  TL  VL+   ++MAPF PF +EV Y+N+ +V    ++S+H  SFP  +       +E++V  M T++ L RN RE+     K PL E+ I+H  A+
Sbjct:  682 KKSPNILDQWVLSRLNGLIANTHKEMDAYRLYNVVPHLLQFIEDLTNTYIRFNRSLFWQDGMPETKRYAYETLHEVLVTLSRLMAPFAPFMSEVTYKNLAQVLKDKKDSVHLESFPTADLSMLRPELEEAVKAMDTLVTLGRNHREKIGVKAKIPLNEIKIIHRSAE 848

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BLAST of HO063403 vs. SwissProt
Match: SYI_CHLCV (Isoleucyl-tRNA synthetase OS=Chlamydophila caviae GN=ileS PE=3 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.024e-22
Identity = 58/158 (36.71%), Postives = 88/158 (55.70%), Query Frame = -3

Query:   15 LDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVCDGSEESIHYCSFPE-EEGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHP 485
            +D+WI S   ++V  VR+ M  Y L T V   + F+D+LTN Y+R  R+R        D R A +TL+ VL + C+V+APF PF +E +YQ ++   + S ES+H C FP  +  K    +EQ +S    I+ L  ++R+ H   ++ PL    IV P
Sbjct:  679 IDRWILSNLYTVVGKVRESMSSYNLNTAVNPFVTFIDDLTNWYIRRCRRRFWESEDTPDRRAAFATLYEVLTVFCRVIAPFIPFISEDIYQQIK--TEHSAESVHLCDFPHIDLAKVFPDLEQRMSDAREIVGLGHSLRKEHKLKVRQPLANFYIVGP 834

[Close]

BLAST of HO063403 vs. SwissProt
Match: SYI_CHLAB (Isoleucyl-tRNA synthetase OS=Chlamydophila abortus GN=ileS PE=3 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 2.289e-21
Identity = 57/162 (35.19%), Postives = 89/162 (54.94%), Query Frame = -3

Query:   15 SSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVCDGSEESIHYCSFPE-EEGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHP 497
            S + +D+WI S   ++V  VR+ M  Y L T V   + F+D+LTN Y+R  R+R        D R A +TL+ VL + C+V+APF PF +E +YQ ++   + S ES+H C FP  +  K    +EQ +     I+ L  ++R+ H   ++ PL    +V P
Sbjct:  675 SYSEIDRWILSNLYTVVGKVRESMSSYNLNTAVNPFVTFIDDLTNWYIRRCRRRFWESADTPDRRAAFATLYEVLTVFCRVIAPFIPFISEDIYQQIK--TENSLESVHLCDFPYIDLAKVFPDLEQRMGDAREIVGLGHSLRKEHKLKVRQPLANFYVVGP 834

[Close]

BLAST of HO063403 vs. TrEMBL
Match: B9HH75_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_216568 PE=3 SV=1)

HSP 1 Score: 314.694 bits (805), Expect = 2.241e-84
Identity = 151/172 (87.79%), Postives = 163/172 (94.77%), Query Frame = -3

Query:    3 DQATLQKSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVCDGSEESIHYCSFPEEEGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHPDADF 518
            D ATLQ SSNVLDQWINSATQSLVHFVRQEM+ YRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTL+NVLL+SCKVMAPFTPFF+E LYQN+R+VC GSEESIHYCSFP+ EG+  ERIEQSV+RMMTIIDLARNIRERHNKPLK+PLREM++VHPD DF
Sbjct:  713 DSATLQDSSNVLDQWINSATQSLVHFVRQEMNAYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLYNVLLISCKVMAPFTPFFSEGLYQNLRRVCTGSEESIHYCSFPQVEGERDERIEQSVARMMTIIDLARNIRERHNKPLKSPLREMIVVHPDVDF 884

[Close]

BLAST of HO063403 vs. TrEMBL
Match: D7U1M5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_102.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00010735001 PE=4 SV=1)

HSP 1 Score: 307.76 bits (787), Expect = 2.740e-82
Identity = 150/172 (87.21%), Postives = 160/172 (93.02%), Query Frame = -3

Query:    3 DQATLQKSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVCDGSEESIHYCSFPEEEGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHPDADF 518
            D  TLQKSSNVLDQWINSATQSLVHFVRQEMD YRLYTVVPYL+KFLD LTN YVRFNRKRLKGRTGE DCRTALSTL+ VLL SCKVMAPFTPFFTEVLYQN+RKV +GSEESIHYCSFP+EEG+ GERIEQSV+RM TIIDLARNIRERHNKP+KTPLREMV+VHPD +F
Sbjct:  669 DGVTLQKSSNVLDQWINSATQSLVHFVRQEMDAYRLYTVVPYLVKFLDYLTNTYVRFNRKRLKGRTGEGDCRTALSTLYYVLLTSCKVMAPFTPFFTEVLYQNLRKVSNGSEESIHYCSFPQEEGQRGERIEQSVARMTTIIDLARNIRERHNKPVKTPLREMVVVHPDQEF 840

[Close]

BLAST of HO063403 vs. TrEMBL
Match: A5AU06_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_034773 PE=3 SV=1)

HSP 1 Score: 307.76 bits (787), Expect = 2.740e-82
Identity = 150/172 (87.21%), Postives = 160/172 (93.02%), Query Frame = -3

Query:    3 DQATLQKSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVCDGSEESIHYCSFPEEEGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHPDADF 518
            D  TLQKSSNVLDQWINSATQSLVHFVRQEMD YRLYTVVPYL+KFLD LTN YVRFNRKRLKGRTGE DCRTALSTL+ VLL SCKVMAPFTPFFTEVLYQN+RKV +GSEESIHYCSFP+EEG+ GERIEQSV+RM TIIDLARNIRERHNKP+KTPLREMV+VHPD +F
Sbjct:  706 DGVTLQKSSNVLDQWINSATQSLVHFVRQEMDAYRLYTVVPYLVKFLDYLTNTYVRFNRKRLKGRTGEGDCRTALSTLYYVLLTSCKVMAPFTPFFTEVLYQNLRKVSNGSEESIHYCSFPQEEGQRGERIEQSVARMTTIIDLARNIRERHNKPVKTPLREMVVVHPDQEF 877

[Close]

BLAST of HO063403 vs. TrEMBL
Match: B9SUT5_RICCO (Isoleucyl tRNA synthetase, putative OS=Ricinus communis GN=RCOM_0574620 PE=3 SV=1)

HSP 1 Score: 306.99 bits (785), Expect = 4.674e-82
Identity = 150/172 (87.21%), Postives = 160/172 (93.02%), Query Frame = -3

Query:    3 DQATLQKSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVCDGSEESIHYCSFPEEEGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHPDADF 518
            D   LQ SSNVLD+WINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGR GEEDCRTALSTL++VLL SCKVM+PFTPFFTEVLYQNMRKV  G+EESIHYCSFP+EEG+  ERIEQSVSRMMTIIDLARNIRERH KPLK+PLREM++VH DADF
Sbjct:  708 DFGKLQNSSNVLDRWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRIGEEDCRTALSTLYSVLLTSCKVMSPFTPFFTEVLYQNMRKVSSGAEESIHYCSFPQEEGERDERIEQSVSRMMTIIDLARNIRERHYKPLKSPLREMIVVHHDADF 879

[Close]

BLAST of HO063403 vs. TrEMBL
Match: B9MY07_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_292610 PE=3 SV=1)

HSP 1 Score: 306.605 bits (784), Expect = 6.104e-82
Identity = 150/176 (85.23%), Postives = 161/176 (91.48%), Query Frame = -3

Query:    3 DQATLQKSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVCDGSEESIHYCSFPEEEGKG----GERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHPDADF 518
            D  TLQ SSNVLDQWINSATQSLVHFV QEM+ YRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTL+NVLL+SCKVMAPFTPFF+E LYQNMR+VC GSEESIHYCSFP+ EG+      ERIEQSV+RMMTIIDLARNIRERHNKPLK+PLREM++VHPD DF
Sbjct:  689 DAGTLQNSSNVLDQWINSATQSLVHFVHQEMNAYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLYNVLLISCKVMAPFTPFFSEGLYQNMRRVCTGSEESIHYCSFPQVEGEVFISLNERIEQSVARMMTIIDLARNIRERHNKPLKSPLREMIVVHPDVDF 864

[Close]

BLAST of HO063403 vs. TrEMBL
Match: D7MJD5_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_916271 PE=4 SV=1)

HSP 1 Score: 291.197 bits (744), Expect = 2.654e-77
Identity = 146/173 (84.39%), Postives = 156/173 (90.17%), Query Frame = -3

Query:    3 DQATLQKSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVCDGSEESIHYCSFPEE-EGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHPDADF 518
            D ATL +SSNVLDQWI SATQSLV FVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGE+DC  ALSTL+NVLL SCKVM PFTPFFTE LYQN+RK C+GSEESIHYCSFPEE EG   ERIEQSV+RMMTIIDLARNIRERH  PLKTPL+E+ +VHPDA+F
Sbjct:  700 DLATL-RSSNVLDQWIYSATQSLVCFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEDDCHIALSTLYNVLLTSCKVMTPFTPFFTETLYQNLRKACEGSEESIHYCSFPEEAEGTRVERIEQSVTRMMTIIDLARNIRERHKLPLKTPLKEITVVHPDAEF 871

[Close]

BLAST of HO063403 vs. TrEMBL
Match: Q9SV89_ARATH (Isoleucine-tRNA ligase-like protein OS=Arabidopsis thaliana GN=F24G24.120 PE=2 SV=1)

HSP 1 Score: 286.96 bits (733), Expect = 5.005e-76
Identity = 140/172 (81.40%), Postives = 153/172 (88.95%), Query Frame = -3

Query:    3 DQATLQKSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVCDGSEESIHYCSFPEEEGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHPDADF 518
            D AT+Q S+N+LDQWI+SATQSLV FVR+EMD YRLYTVVP LLKFLDNLTNIYVRFNRKRLKGRTGE+DC TALSTLFNVLL SCKVMAPFTPFFTE LYQN+RK C GSEES+HYCS P  EG  GERIE SV+RMM IIDLARNIRER+  PLKTPL+EM++VHPDADF
Sbjct:  714 DLATIQ-SANILDQWIHSATQSLVRFVREEMDAYRLYTVVPRLLKFLDNLTNIYVRFNRKRLKGRTGEDDCHTALSTLFNVLLTSCKVMAPFTPFFTETLYQNLRKACKGSEESVHYCSIPPREGMEGERIELSVTRMMKIIDLARNIRERNKLPLKTPLKEMIVVHPDADF 884

[Close]

BLAST of HO063403 vs. TrEMBL
Match: O82613_ARATH (T9A4.4 protein OS=Arabidopsis thaliana GN=T9A4.4 PE=4 SV=1)

HSP 1 Score: 286.96 bits (733), Expect = 5.005e-76
Identity = 140/172 (81.40%), Postives = 153/172 (88.95%), Query Frame = -3

Query:    3 DQATLQKSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVCDGSEESIHYCSFPEEEGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHPDADF 518
            D AT+Q S+N+LDQWI+SATQSLV FVR+EMD YRLYTVVP LLKFLDNLTNIYVRFNRKRLKGRTGE+DC TALSTLFNVLL SCKVMAPFTPFFTE LYQN+RK C GSEES+HYCS P  EG  GERIE SV+RMM IIDLARNIRER+  PLKTPL+EM++VHPDADF
Sbjct:  122 DLATIQ-SANILDQWIHSATQSLVRFVREEMDAYRLYTVVPRLLKFLDNLTNIYVRFNRKRLKGRTGEDDCHTALSTLFNVLLTSCKVMAPFTPFFTETLYQNLRKACKGSEESVHYCSIPPREGMEGERIELSVTRMMKIIDLARNIRERNKLPLKTPLKEMIVVHPDADF 292

[Close]

BLAST of HO063403 vs. TrEMBL
Match: D7LY38_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_489875 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.325e-73
Identity = 135/172 (78.49%), Postives = 154/172 (89.53%), Query Frame = -3

Query:    3 DQATLQKSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVCDGSEESIHYCSFPEEEGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHPDADF 518
            D ATLQ S+NVLDQWI+SATQSLV  VR+EM  Y+LYTVVP+LLKFLD+LTN+YVRFNRKRLKGRTGE+DC TALSTLFNVLL +CKVMAPFTPFFTE LYQN+RK C GSEES+HYCS P +EGK G+RIE SV+RMM IIDLARNIRER+  PLKTPL+EM++VHPDA+F
Sbjct:  714 DLATLQ-SANVLDQWIHSATQSLVRTVRREMKKYQLYTVVPHLLKFLDSLTNVYVRFNRKRLKGRTGEDDCHTALSTLFNVLLTTCKVMAPFTPFFTETLYQNLRKACKGSEESVHYCSIPPKEGKEGKRIEDSVTRMMKIIDLARNIRERNKLPLKTPLKEMIVVHPDAEF 884

[Close]

BLAST of HO063403 vs. TrEMBL
Match: C7IYG2_ORYSJ (Os02g0183700 protein OS=Oryza sativa subsp. japonica GN=Os02g0183700 PE=4 SV=1)

HSP 1 Score: 265.003 bits (676), Expect = 2.037e-69
Identity = 130/188 (69.15%), Postives = 152/188 (80.85%), Query Frame = -3

Query:    3 DQATLQKSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVCDGSEESIHYCSFPEEEGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLR----------------EMVIVHPDADF 518
            +QA L +SSNVLD WI SAT+SLV FVRQEMD YRLYTVVPYL+K++DNLTNIYVRFNRKRLKGRTGE+DCR +LSTL++ L+ +C VMAPFTPFFTEVLYQN+RKV   SE+SIH+C+FP   G+  ER+E SV+RMMTI+DLARNIRERHN+PLKTPLR                EMV+VHPD DF
Sbjct:  674 NQAILLRSSNVLDHWIQSATESLVSFVRQEMDAYRLYTVVPYLVKYIDNLTNIYVRFNRKRLKGRTGEDDCRISLSTLYHALVTTCVVMAPFTPFFTEVLYQNLRKVSSKSEKSIHFCNFPSSTGQIDERVEHSVNRMMTIVDLARNIRERHNRPLKTPLRHAHYTTILFTQFLVLWEMVVVHPDNDF 861

[Close]

BLAST of HO063403 vs. TAIR peptide
Match: AT4G10320.1 (| Symbols: | tRNA synthetase class I (I, L, M and V) family protein | chr4:6397526-6404509 REVERSE LENGTH=1190)

HSP 1 Score: 286.96 bits (733), Expect = 4.153e-78
Identity = 140/172 (81.40%), Postives = 153/172 (88.95%), Query Frame = -3

Query:    3 DQATLQKSSNVLDQWINSATQSLVHFVRQEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRTALSTLFNVLLLSCKVMAPFTPFFTEVLYQNMRKVCDGSEESIHYCSFPEEEGKGGERIEQSVSRMMTIIDLARNIRERHNKPLKTPLREMVIVHPDADF 518
            D AT+Q S+N+LDQWI+SATQSLV FVR+EMD YRLYTVVP LLKFLDNLTNIYVRFNRKRLKGRTGE+DC TALSTLFNVLL SCKVMAPFTPFFTE LYQN+RK C GSEES+HYCS P  EG  GERIE SV+RMM IIDLARNIRER+  PLKTPL+EM++VHPDADF
Sbjct:  714 DLATIQ-SANILDQWIHSATQSLVRFVREEMDAYRLYTVVPRLLKFLDNLTNIYVRFNRKRLKGRTGEDDCHTALSTLFNVLLTSCKVMAPFTPFFTETLYQNLRKACKGSEESVHYCSIPPREGMEGERIELSVTRMMKIIDLARNIRERNKLPLKTPLKEMIVVHPDADF 884

The following BLAST results are available for this feature:

BLAST of HO063403 vs. SwissProt
BLAST of HO063403 vs. TrEMBL
BLAST of HO063403 vs. TAIR peptide

BLAST of HO063403 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
SYIC_MOUSE	2.352e-42	48.33	Isoleucyl-tRNA synthetase, cytoplasmic OS=Mus musc...	[more]
SYIC_HUMAN	3.072e-42	48.89	Isoleucyl-tRNA synthetase, cytoplasmic OS=Homo sap...	[more]
SYIC_DICDI	1.168e-41	50.00	Probable isoleucyl-tRNA synthetase, cytoplasmic OS...	[more]
SYIC_CAEEL	3.756e-40	47.43	Isoleucyl-tRNA synthetase, cytoplasmic OS=Caenorha...	[more]
SYIC_YEAST	6.406e-40	48.02	Isoleucyl-tRNA synthetase, cytoplasmic OS=Saccharo...	[more]
SYIC_SCHPO	1.477e-36	45.30	Isoleucyl-tRNA synthetase, cytoplasmic OS=Schizosa...	[more]
SYI_TETTH	1.805e-34	45.61	Isoleucyl-tRNA synthetase OS=Tetrahymena thermophi...	[more]
SYI_BDEBA	2.698e-30	41.32	Isoleucyl-tRNA synthetase OS=Bdellovibrio bacterio...	[more]
SYI_CHLCV	6.024e-22	36.71	Isoleucyl-tRNA synthetase OS=Chlamydophila caviae ...	[more]
SYI_CHLAB	2.289e-21	35.19	Isoleucyl-tRNA synthetase OS=Chlamydophila abortus...	[more]

BLAST of HO063403 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9HH75_POPTR	2.241e-84	87.79	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
D7U1M5_VITVI	2.740e-82	87.21	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5AU06_VITVI	2.740e-82	87.21	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9SUT5_RICCO	4.674e-82	87.21	Isoleucyl tRNA synthetase, putative OS=Ricinus com...	[more]
B9MY07_POPTR	6.104e-82	85.23	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
D7MJD5_ARALY	2.654e-77	84.39	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q9SV89_ARATH	5.005e-76	81.40	Isoleucine-tRNA ligase-like protein OS=Arabidopsis...	[more]
O82613_ARATH	5.005e-76	81.40	T9A4.4 protein OS=Arabidopsis thaliana GN=T9A4.4 P...	[more]
D7LY38_ARALY	2.325e-73	78.49	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
C7IYG2_ORYSJ	2.037e-69	69.15	Os02g0183700 protein OS=Oryza sativa subsp. japoni...	[more]

BLAST of HO063403 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G10320.1	4.153e-78	81.40	\| Symbols: \| tRNA synthetase class I (I, L, M and...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002301	Isoleucyl-tRNA synthetase, class Ia	PANTHER	PTHR11946:SF9	ISOLEUCYL TRNA SYNTHETASE	coord: 10..176 score: 2.3
IPR009080	Aminoacyl-tRNA synthetase, class 1a, anticodon-binding	SUPERFAMILY	47323	Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases	coord: 3..156 score: 2.1
IPR013155	Valyl/Leucyl/Isoleucyl-tRNA synthetase, class I, anticodon-binding	PFAM	PF08264	Anticodon_1	coord: 16..165 score: 5.4
None	No IPR available	GENE3D	1.10.730.10	no description	coord: 8..156 score: 7.2
None	No IPR available	PANTHER	PTHR11946	ISOLEUCYL, LEUCYL, TYROSYL, VALYL AND METHIONYL-TRNA SYNTHETASES	coord: 10..176 score: 2.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO063403 ID=HO063403; Name=HO063403; organism=Cicer arietinum; type=EST; length=529bp
AGGAAGTCTGCATCAGGATGCACTATCACCATCTCCCTGAGTGGTGTTTT
AAGAGGCTTGTTATGACGCTCACGAATGTTTCGGGCCAGATCAATTATTG
TCATCATCCTTGAAACACTTTGCTCAATCCGCTCTCCCCCCTTGCCTTCT
TCCTCAGGAAAACTGCAATAGTGTATGCTCTCCTCTGATCCATCACAAAC
TTTTCGCATATTTTGATAGAGCACCTCAGTGAAGAAAGGTGTAAAAGGAG
CCATCACTTTACAGGATAATAAAAGCACGTTGAAAAGAGTTGAAAGTGCT
GTCCTGCAGTCTTCTTCTCCAGTACGACCTTTCAGTCTCTTACGGTTGAA
CCGCACATATATATTTGTAAGGTTATCAAGAAATTTTAGAAGATATGGAA
CCACCGTATAAAGGCGGTAACCATCCATTTCTTGCCGTACAAAATGAACA
AGGCTTTGTGTGGCTGAGTTGATCCACTGATCAAGAACATTTGATGACTT
CTGAAGTGTGGCCTGATCAAATAGTACCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0000166	nucleotide binding
GO:0004822	isoleucine-tRNA ligase activity
GO:0005524	ATP binding
GO:0004812	aminoacyl-tRNA ligase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation
GO:0006428	isoleucyl-tRNA aminoacylation
GO:0006418	tRNA aminoacylation for protein translation

Vocabulary: **INTERPRO**
Term	Definition
IPR002301	Ile-tRNA-ligase
IPR009080	tRNAsynth_Ia_anticodon-bd
IPR013155	M/V/L/I-tRNA-synth_anticd-bd

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