HO066361

EST Overview


Name	HO066361
Unique Name	HO066361
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	713

Libraries


Library Name	Type
LIBEST_026413 Chickpea drought stressed cDNA SSH library AS2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO066361 vs. SwissProt
Match: CHLM_SYNY3 (Magnesium-protoporphyrin O-methyltransferase OS=Synechocystis sp. (strain PCC 6803) GN=chlM PE=3 SV=1)

HSP 1 Score: 44.669 bits (104), Expect = 5.845e-8
Identity = 20/32 (62.50%), Postives = 22/32 (68.75%), Query Frame = -3

Query:  382 KTFYYDALKRVGELFPGPSKATRAYLHSEGDI 477
            KT     LK++G LFPGPSK TRAY H E DI
Sbjct:  167 KTLGLTVLKKIGGLFPGPSKTTRAYQHKEADI 198

HSP 2 Score: 33.113 bits (74), Expect = 5.845e-8
Identity = 12/23 (52.17%), Postives = 21/23 (91.30%), Query Frame = -2

Query:  293 GWTIKKRGLTTTQFYFSRLIEAV 361
            G++I + G+T+T+FY+SR++EAV
Sbjct:  206 GFSIARTGMTSTRFYYSRILEAV 228

[Close]

BLAST of HO066361 vs. TrEMBL
Match: Q9SW18_ARATH (AT4G25080 protein OS=Arabidopsis thaliana GN=F13M23.220 PE=2 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.350e-18
Identity = 28/32 (87.50%), Postives = 30/32 (93.75%), Query Frame = -3

Query:  382 KTFYYDALKRVGELFPGPSKATRAYLHSEGDI 477
            KTFYYD LKR+GELFPGPSKATRAYLHSE D+
Sbjct:  249 KTFYYDILKRIGELFPGPSKATRAYLHSEADV 280

HSP 2 Score: 52.7582 bits (125), Expect = 1.350e-18
Identity = 24/26 (92.31%), Postives = 24/26 (92.31%), Query Frame = -2

Query:  287 VGWTIKKRGLTTTQFYFSRLIEAVPM 364
            VGW I KRGLTTTQFYFSRLIEAVPM
Sbjct:  287 VGWKISKRGLTTTQFYFSRLIEAVPM 312

[Close]

BLAST of HO066361 vs. TrEMBL
Match: D7MGJ7_ARALY (Magnesium-protoporphyrin ix methyltransferase OS=Arabidopsis lyrata subsp. lyrata GN=CHLM PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.350e-18
Identity = 28/32 (87.50%), Postives = 30/32 (93.75%), Query Frame = -3

Query:  382 KTFYYDALKRVGELFPGPSKATRAYLHSEGDI 477
            KTFYYD LKR+GELFPGPSKATRAYLHSE D+
Sbjct:  249 KTFYYDILKRIGELFPGPSKATRAYLHSEADV 280

HSP 2 Score: 52.7582 bits (125), Expect = 1.350e-18
Identity = 24/26 (92.31%), Postives = 24/26 (92.31%), Query Frame = -2

Query:  287 VGWTIKKRGLTTTQFYFSRLIEAVPM 364
            VGW I KRGLTTTQFYFSRLIEAVPM
Sbjct:  287 VGWKISKRGLTTTQFYFSRLIEAVPM 312

[Close]

BLAST of HO066361 vs. TrEMBL
Match: B9DF98_ARATH (AT4G25080 protein OS=Arabidopsis thaliana GN=AT4G25080 PE=2 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.350e-18
Identity = 28/32 (87.50%), Postives = 30/32 (93.75%), Query Frame = -3

Query:  382 KTFYYDALKRVGELFPGPSKATRAYLHSEGDI 477
            KTFYYD LKR+GELFPGPSKATRAYLHSE D+
Sbjct:  249 KTFYYDILKRIGELFPGPSKATRAYLHSEADV 280

HSP 2 Score: 52.7582 bits (125), Expect = 1.350e-18
Identity = 24/26 (92.31%), Postives = 24/26 (92.31%), Query Frame = -2

Query:  287 VGWTIKKRGLTTTQFYFSRLIEAVPM 364
            VGW I KRGLTTTQFYFSRLIEAVPM
Sbjct:  287 VGWKISKRGLTTTQFYFSRLIEAVPM 312

[Close]

BLAST of HO066361 vs. TrEMBL
Match: B3H4K6_ARATH (AT4G25080 protein OS=Arabidopsis thaliana GN=AT4G25080 PE=2 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.360e-18
Identity = 28/32 (87.50%), Postives = 30/32 (93.75%), Query Frame = -3

Query:  382 KTFYYDALKRVGELFPGPSKATRAYLHSEGDI 477
            KTFYYD LKR+GELFPGPSKATRAYLHSE D+
Sbjct:  182 KTFYYDILKRIGELFPGPSKATRAYLHSEADV 213

HSP 2 Score: 52.7582 bits (125), Expect = 1.360e-18
Identity = 24/26 (92.31%), Postives = 24/26 (92.31%), Query Frame = -2

Query:  287 VGWTIKKRGLTTTQFYFSRLIEAVPM 364
            VGW I KRGLTTTQFYFSRLIEAVPM
Sbjct:  220 VGWKISKRGLTTTQFYFSRLIEAVPM 245

[Close]

BLAST of HO066361 vs. TrEMBL
Match: B9I3Z4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_662234 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 7.092e-17
Identity = 28/32 (87.50%), Postives = 30/32 (93.75%), Query Frame = -3

Query:  382 KTFYYDALKRVGELFPGPSKATRAYLHSEGDI 477
            KTFYYD LKRVGELFPGPSKATRAYLH+E D+
Sbjct:  119 KTFYYDLLKRVGELFPGPSKATRAYLHAEADV 150

HSP 2 Score: 47.7506 bits (112), Expect = 7.092e-17
Identity = 19/25 (76.00%), Postives = 23/25 (92.00%), Query Frame = -2

Query:  290 VGWTIKKRGLTTTQFYFSRLIEAVP 364
            VGW I+KRGL TTQFYF+RL+EA+P
Sbjct:  157 VGWKIRKRGLVTTQFYFARLVEAIP 181

[Close]

BLAST of HO066361 vs. TrEMBL
Match: D7SH73_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_0.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00008643001 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.525e-16
Identity = 28/32 (87.50%), Postives = 30/32 (93.75%), Query Frame = -3

Query:  382 KTFYYDALKRVGELFPGPSKATRAYLHSEGDI 477
            KTFYYD LKRVGELFPGPSKATRAYLH+E D+
Sbjct:  254 KTFYYDLLKRVGELFPGPSKATRAYLHAEADV 285

HSP 2 Score: 46.595 bits (109), Expect = 1.525e-16
Identity = 19/25 (76.00%), Postives = 23/25 (92.00%), Query Frame = -2

Query:  290 VGWTIKKRGLTTTQFYFSRLIEAVP 364
            VGW I+KRGL TTQFYF++L+EAVP
Sbjct:  292 VGWRIRKRGLITTQFYFAKLVEAVP 316

[Close]

BLAST of HO066361 vs. TrEMBL
Match: B9RA68_RICCO (Magnesium-protoporphyrin O-methyltransferase, putative OS=Ricinus communis GN=RCOM_1503660 PE=4 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 9.569e-16
Identity = 27/32 (84.38%), Postives = 30/32 (93.75%), Query Frame = -3

Query:  382 KTFYYDALKRVGELFPGPSKATRAYLHSEGDI 477
            KTFYY+ LKRVGELFPGPSKATRAYLH+E D+
Sbjct:  252 KTFYYNLLKRVGELFPGPSKATRAYLHAEADV 283

HSP 2 Score: 45.8246 bits (107), Expect = 9.569e-16
Identity = 19/25 (76.00%), Postives = 22/25 (88.00%), Query Frame = -2

Query:  290 VGWTIKKRGLTTTQFYFSRLIEAVP 364
            VGW I+ RGL TTQFYF+RL+EAVP
Sbjct:  290 VGWRIRNRGLITTQFYFARLVEAVP 314

[Close]

BLAST of HO066361 vs. TrEMBL
Match: Q9MUE2_TOBAC (S-adenosyl-L-methionine Mg-protoporphyrin IX methyltranserase OS=Nicotiana tabacum GN=Chl M PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.615e-15
Identity = 27/32 (84.38%), Postives = 30/32 (93.75%), Query Frame = -3

Query:  382 KTFYYDALKRVGELFPGPSKATRAYLHSEGDI 477
            KTFYYD LKR+GELFPGPSKATRAYLH+E D+
Sbjct:  262 KTFYYDLLKRIGELFPGPSKATRAYLHAEADV 293

HSP 2 Score: 43.5134 bits (101), Expect = 1.615e-15
Identity = 18/24 (75.00%), Postives = 21/24 (87.50%), Query Frame = -2

Query:  290 GWTIKKRGLTTTQFYFSRLIEAVP 361
            GW I+KRGL  TQFYF++LIEAVP
Sbjct:  301 GWKIRKRGLIATQFYFAKLIEAVP 324

[Close]

BLAST of HO066361 vs. TrEMBL
Match: A9NUI2_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 2.344e-13
Identity = 25/32 (78.12%), Postives = 28/32 (87.50%), Query Frame = -3

Query:  382 KTFYYDALKRVGELFPGPSKATRAYLHSEGDI 477
            KT+YY  LKR+GELFPGPSK TRAYLHSE D+
Sbjct:  262 KTWYYSVLKRIGELFPGPSKTTRAYLHSEEDV 293

HSP 2 Score: 41.2022 bits (95), Expect = 2.344e-13
Identity = 15/24 (62.50%), Postives = 21/24 (87.50%), Query Frame = -2

Query:  290 GWTIKKRGLTTTQFYFSRLIEAVP 361
            GW +++R +T T+FYFSRL+EAVP
Sbjct:  301 GWVVRRRDMTATKFYFSRLLEAVP 324

[Close]

BLAST of HO066361 vs. TrEMBL
Match: A9NMT9_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 2.344e-13
Identity = 25/32 (78.12%), Postives = 28/32 (87.50%), Query Frame = -3

Query:  382 KTFYYDALKRVGELFPGPSKATRAYLHSEGDI 477
            KT+YY  LKR+GELFPGPSK TRAYLHSE D+
Sbjct:  262 KTWYYSVLKRIGELFPGPSKTTRAYLHSEEDV 293

HSP 2 Score: 41.2022 bits (95), Expect = 2.344e-13
Identity = 15/24 (62.50%), Postives = 21/24 (87.50%), Query Frame = -2

Query:  290 GWTIKKRGLTTTQFYFSRLIEAVP 361
            GW +++R +T T+FYFSRL+EAVP
Sbjct:  301 GWVVRRRDMTATKFYFSRLLEAVP 324

[Close]

BLAST of HO066361 vs. TAIR peptide
Match: AT4G25080.5 (| Symbols: CHLM | magnesium-protoporphyrin IX methyltransferase | chr4:12877015-12878128 FORWARD LENGTH=312)

HSP 1 Score: 65.0846 bits (157), Expect = 9.064e-21
Identity = 28/32 (87.50%), Postives = 30/32 (93.75%), Query Frame = -3

Query:  382 KTFYYDALKRVGELFPGPSKATRAYLHSEGDI 477
            KTFYYD LKR+GELFPGPSKATRAYLHSE D+
Sbjct:  249 KTFYYDILKRIGELFPGPSKATRAYLHSEADV 280

HSP 2 Score: 52.7582 bits (125), Expect = 9.064e-21
Identity = 24/26 (92.31%), Postives = 24/26 (92.31%), Query Frame = -2

Query:  287 VGWTIKKRGLTTTQFYFSRLIEAVPM 364
            VGW I KRGLTTTQFYFSRLIEAVPM
Sbjct:  287 VGWKISKRGLTTTQFYFSRLIEAVPM 312

[Close]

BLAST of HO066361 vs. TAIR peptide
Match: AT4G25080.2 (| Symbols: CHLM | magnesium-protoporphyrin IX methyltransferase | chr4:12877015-12878128 FORWARD LENGTH=312)

HSP 1 Score: 65.0846 bits (157), Expect = 9.064e-21
Identity = 28/32 (87.50%), Postives = 30/32 (93.75%), Query Frame = -3

Query:  382 KTFYYDALKRVGELFPGPSKATRAYLHSEGDI 477
            KTFYYD LKR+GELFPGPSKATRAYLHSE D+
Sbjct:  249 KTFYYDILKRIGELFPGPSKATRAYLHSEADV 280

HSP 2 Score: 52.7582 bits (125), Expect = 9.064e-21
Identity = 24/26 (92.31%), Postives = 24/26 (92.31%), Query Frame = -2

Query:  287 VGWTIKKRGLTTTQFYFSRLIEAVPM 364
            VGW I KRGLTTTQFYFSRLIEAVPM
Sbjct:  287 VGWKISKRGLTTTQFYFSRLIEAVPM 312

[Close]

BLAST of HO066361 vs. TAIR peptide
Match: AT4G25080.1 (| Symbols: CHLM | magnesium-protoporphyrin IX methyltransferase | chr4:12877015-12878128 FORWARD LENGTH=312)

HSP 1 Score: 65.0846 bits (157), Expect = 9.064e-21
Identity = 28/32 (87.50%), Postives = 30/32 (93.75%), Query Frame = -3

Query:  382 KTFYYDALKRVGELFPGPSKATRAYLHSEGDI 477
            KTFYYD LKR+GELFPGPSKATRAYLHSE D+
Sbjct:  249 KTFYYDILKRIGELFPGPSKATRAYLHSEADV 280

HSP 2 Score: 52.7582 bits (125), Expect = 9.064e-21
Identity = 24/26 (92.31%), Postives = 24/26 (92.31%), Query Frame = -2

Query:  287 VGWTIKKRGLTTTQFYFSRLIEAVPM 364
            VGW I KRGLTTTQFYFSRLIEAVPM
Sbjct:  287 VGWKISKRGLTTTQFYFSRLIEAVPM 312

[Close]

BLAST of HO066361 vs. TAIR peptide
Match: AT4G25080.3 (| Symbols: CHLM | magnesium-protoporphyrin IX methyltransferase | chr4:12877015-12878128 FORWARD LENGTH=312)

HSP 1 Score: 65.0846 bits (157), Expect = 9.064e-21
Identity = 28/32 (87.50%), Postives = 30/32 (93.75%), Query Frame = -3

Query:  382 KTFYYDALKRVGELFPGPSKATRAYLHSEGDI 477
            KTFYYD LKR+GELFPGPSKATRAYLHSE D+
Sbjct:  249 KTFYYDILKRIGELFPGPSKATRAYLHSEADV 280

HSP 2 Score: 52.7582 bits (125), Expect = 9.064e-21
Identity = 24/26 (92.31%), Postives = 24/26 (92.31%), Query Frame = -2

Query:  287 VGWTIKKRGLTTTQFYFSRLIEAVPM 364
            VGW I KRGLTTTQFYFSRLIEAVPM
Sbjct:  287 VGWKISKRGLTTTQFYFSRLIEAVPM 312

[Close]

BLAST of HO066361 vs. TAIR peptide
Match: AT4G25080.4 (| Symbols: CHLM | magnesium-protoporphyrin IX methyltransferase | chr4:12877216-12878128 FORWARD LENGTH=245)

HSP 1 Score: 65.0846 bits (157), Expect = 9.118e-21
Identity = 28/32 (87.50%), Postives = 30/32 (93.75%), Query Frame = -3

Query:  382 KTFYYDALKRVGELFPGPSKATRAYLHSEGDI 477
            KTFYYD LKR+GELFPGPSKATRAYLHSE D+
Sbjct:  182 KTFYYDILKRIGELFPGPSKATRAYLHSEADV 213

HSP 2 Score: 52.7582 bits (125), Expect = 9.118e-21
Identity = 24/26 (92.31%), Postives = 24/26 (92.31%), Query Frame = -2

Query:  287 VGWTIKKRGLTTTQFYFSRLIEAVPM 364
            VGW I KRGLTTTQFYFSRLIEAVPM
Sbjct:  220 VGWKISKRGLTTTQFYFSRLIEAVPM 245

The following BLAST results are available for this feature:

BLAST of HO066361 vs. SwissProt
BLAST of HO066361 vs. TrEMBL
BLAST of HO066361 vs. TAIR peptide

BLAST of HO066361 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 1


Match Name	E-value	Identity	Description
CHLM_SYNY3	5.845e-8	62.50	Magnesium-protoporphyrin O-methyltransferase OS=Sy...	[more]

BLAST of HO066361 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9SW18_ARATH	1.350e-18	87.50	AT4G25080 protein OS=Arabidopsis thaliana GN=F13M2...	[more]
D7MGJ7_ARALY	1.350e-18	87.50	Magnesium-protoporphyrin ix methyltransferase OS=A...	[more]
B9DF98_ARATH	1.350e-18	87.50	AT4G25080 protein OS=Arabidopsis thaliana GN=AT4G2...	[more]
B3H4K6_ARATH	1.360e-18	87.50	AT4G25080 protein OS=Arabidopsis thaliana GN=AT4G2...	[more]
B9I3Z4_POPTR	7.092e-17	87.50	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7SH73_VITVI	1.525e-16	87.50	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9RA68_RICCO	9.569e-16	84.38	Magnesium-protoporphyrin O-methyltransferase, puta...	[more]
Q9MUE2_TOBAC	1.615e-15	84.38	S-adenosyl-L-methionine Mg-protoporphyrin IX methy...	[more]
A9NUI2_PICSI	2.344e-13	78.13	Putative uncharacterized protein OS=Picea sitchens...	[more]
A9NMT9_PICSI	2.344e-13	78.13	Putative uncharacterized protein OS=Picea sitchens...	[more]

BLAST of HO066361 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT4G25080.5	9.064e-21	87.50	\| Symbols: CHLM \| magnesium-protoporphyrin IX meth...	[more]
AT4G25080.2	9.064e-21	87.50	\| Symbols: CHLM \| magnesium-protoporphyrin IX meth...	[more]
AT4G25080.1	9.064e-21	87.50	\| Symbols: CHLM \| magnesium-protoporphyrin IX meth...	[more]
AT4G25080.3	9.064e-21	87.50	\| Symbols: CHLM \| magnesium-protoporphyrin IX meth...	[more]
AT4G25080.4	9.118e-21	87.50	\| Symbols: CHLM \| magnesium-protoporphyrin IX meth...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010940	Magnesium-protoporphyrin IX methyltransferase, C-terminal	PFAM	PF07109	Mg-por_mtran_C	coord: 50..89 score: 8

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO066361 ID=HO066361; Name=HO066361; organism=Cicer arietinum; type=EST; length=713bp
AATTTCAAATTTGAAATTTTTTCCAATTACAAAAAAAAATTACACTAATT
CCTTTTACATTGAGAAGAAATAAAACATACATATCCAGCAAATCATAGGA
TGACATAACATACAAAAGCACAACAAAAAATGGCTTACAATATACATACG
GAATTTCCTCTAAATAGGGTTGGCTACATGGGATCAAATTATGTCAGTGT
TCTGTCAATTATTACGTATTCGATCCATGTCGAAGGAATTTCCTTTAATG
ATGCAAAACTTACCTCTGAAGCCTCTGGCTTCGCTACATAGGAACAGCCT
CGATAAGCCTGGAAAAGTAAAATTGAGTAGTGGTTAAGCCTCTCTTCTTT
ATAGTCCACCCAACTTACGCAGTGCCCTTTCAATATCTCCCTCGGAGTGA
AGATATGCCCTGGTTGCCTTGGAAGGCCCAGGGAACAACTCACCAACCCT
CTTCAGCGCATCATAATAAAATGTCTTAGTGCCCGCGTACCTGCCCGGGG
CGGCCGCTCGAAAATCCGAATTCCCCCGCGGGCGACATGGGCGGGCCGGG
GAGCCATAGCAGACAGGTACGGGCCCAAATTCGCCCCTAAAAGTAGAGTC
GAATACAATCCAATGGNCCGACCGTTAACCACCGACGGAACTTGGGGAAA
ACCCTGGGTAACCCAATNATCGCTGCAGACATACCCTTTCGCAAAGCGTA
AACAAAAAGNCCG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0015979	photosynthesis
GO:0015995	chlorophyll biosynthetic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0046406	magnesium protoporphyrin IX methyltransferase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR010940	Mg_prot_MeTrfase_C

Search form