HO066383

EST Overview
NameHO066383
Unique NameHO066383
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length842
Libraries
Library NameType
LIBEST_026413 Chickpea drought stressed cDNA SSH library AS2cdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
InterProScan analysis for Cicer arietinum unigene v12010-12-29
Homology
[Close]
BLAST of HO066383 vs. TrEMBL
Match: C6TCM2_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.263e-38
Identity = 42/43 (97.67%), Postives = 43/43 (100.00%), Query Frame = -1 
Query:  225 LWNPHLQMEACYKDFVCVENAKIGSVQLEPEQTWTAVQHLSIA 353
            LWNP+LQMEACYKDFVCVENAKIGSVQLEPEQTWTAVQHLSIA
Sbjct:  269 LWNPYLQMEACYKDFVCVENAKIGSVQLEPEQTWTAVQHLSIA 311

HSP 2 Score: 92.4337 bits (228), Expect = 1.263e-38
Identity = 43/48 (89.58%), Postives = 45/48 (93.75%), Query Frame = -3 
Query:  343 EERDVVTFPGFVDCVYLDASNELQLDNGLGDLISIKNTNWSDSVFVEP 486
            EERDVVTFPGFVDCVYLDAS+ELQLDNGLGDLISIKNTNWSD+V   P
Sbjct:  225 EERDVVTFPGFVDCVYLDASSELQLDNGLGDLISIKNTNWSDAVLWNP 272
[Close]
BLAST of HO066383 vs. TrEMBL
Match: D7SPC0_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_23.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00018938001 PE=4 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 2.925e-34
Identity = 37/48 (77.08%), Postives = 42/48 (87.50%), Query Frame = -3 
Query:  343 EERDVVTFPGFVDCVYLDASNELQLDNGLGDLISIKNTNWSDSVFVEP 486
            EERDVVTFPGFVDC+YLDA N L LDNGLGD+I++KNTNWSD+V   P
Sbjct:  218 EERDVVTFPGFVDCIYLDAPNALHLDNGLGDIITVKNTNWSDTVLWNP 265

HSP 2 Score: 85.5001 bits (210), Expect = 2.925e-34
Identity = 36/42 (85.71%), Postives = 41/42 (97.62%), Query Frame = -1 
Query:  228 LWNPHLQMEACYKDFVCVENAKIGSVQLEPEQTWTAVQHLSI 353
            LWNP+LQMEACY+DFVCVENAKIG VQLEPEQ+W+A+QHLSI
Sbjct:  262 LWNPYLQMEACYRDFVCVENAKIGKVQLEPEQSWSAIQHLSI 303
[Close]
BLAST of HO066383 vs. TrEMBL
Match: B9HF22_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_719543 PE=4 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 3.158e-33
Identity = 37/42 (88.10%), Postives = 41/42 (97.62%), Query Frame = -1 
Query:  228 LWNPHLQMEACYKDFVCVENAKIGSVQLEPEQTWTAVQHLSI 353
            LWNPHLQMEACYKDFVCVENA+IG+VQLEP+Q+WTA QHLSI
Sbjct:  252 LWNPHLQMEACYKDFVCVENAQIGNVQLEPQQSWTAKQHLSI 293

HSP 2 Score: 81.2629 bits (199), Expect = 3.158e-33
Identity = 38/48 (79.17%), Postives = 42/48 (87.50%), Query Frame = -3 
Query:  343 EERDVVTFPGFVDCVYLDASNELQLDNGLGDLISIKNTNWSDSVFVEP 486
            EERDVV FPGFVD VYLDA +ELQLDNGLGD+I+IKNTNWSD+V   P
Sbjct:  208 EERDVVIFPGFVDRVYLDAPSELQLDNGLGDVITIKNTNWSDTVLWNP 255
[Close]
BLAST of HO066383 vs. TrEMBL
Match: D7MMG0_ARALY (Aldose 1-epimerase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_920075 PE=4 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 5.771e-32
Identity = 40/48 (83.33%), Postives = 41/48 (85.42%), Query Frame = -3 
Query:  343 EERDVVTFPGFVDCVYLDASNELQLDNGLGDLISIKNTNWSDSVFVEP 486
            EERDVVTFPGFVDCVYLDA NELQ DNGLGD I IKNTNWSD+V   P
Sbjct:  221 EERDVVTFPGFVDCVYLDAPNELQFDNGLGDNIIIKNTNWSDAVLWNP 268

HSP 2 Score: 77.411 bits (189), Expect = 5.771e-32
Identity = 33/43 (76.74%), Postives = 38/43 (88.37%), Query Frame = -1 
Query:  225 LWNPHLQMEACYKDFVCVENAKIGSVQLEPEQTWTAVQHLSIA 353
            LWNPH QMEACY+DFVCVENAK+G V+LEP Q+WTA Q LSI+
Sbjct:  265 LWNPHTQMEACYRDFVCVENAKLGDVKLEPGQSWTATQLLSIS 307
[Close]
BLAST of HO066383 vs. TrEMBL
Match: Q9FJY6_ARATH (Apospory-associated protein C-like OS=Arabidopsis thaliana GN=At5g66530 PE=1 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 3.949e-30
Identity = 37/48 (77.08%), Postives = 39/48 (81.25%), Query Frame = -3 
Query:  343 EERDVVTFPGFVDCVYLDASNELQLDNGLGDLISIKNTNWSDSVFVEP 486
            E+RD VTFPGFVD VYLDA NELQ DNGLGD I IKNTNWSD+V   P
Sbjct:  221 EDRDAVTFPGFVDTVYLDAPNELQFDNGLGDKIIIKNTNWSDAVLWNP 268

HSP 2 Score: 77.411 bits (189), Expect = 3.949e-30
Identity = 33/43 (76.74%), Postives = 38/43 (88.37%), Query Frame = -1 
Query:  225 LWNPHLQMEACYKDFVCVENAKIGSVQLEPEQTWTAVQHLSIA 353
            LWNPH QMEACY+DFVCVENAK+G V+LEP Q+WTA Q LSI+
Sbjct:  265 LWNPHTQMEACYRDFVCVENAKLGDVKLEPGQSWTATQLLSIS 307
[Close]
BLAST of HO066383 vs. TrEMBL
Match: A9NZI3_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.218e-28
Identity = 32/42 (76.19%), Postives = 38/42 (90.48%), Query Frame = -1 
Query:  228 LWNPHLQMEACYKDFVCVENAKIGSVQLEPEQTWTAVQHLSI 353
            LWNPH+QMEACYKDFVCVENAKI +V+LEP QTW+  Q+LS+
Sbjct:  317 LWNPHMQMEACYKDFVCVENAKIANVELEPGQTWSGEQYLSV 358

HSP 2 Score: 74.3294 bits (181), Expect = 1.218e-28
Identity = 32/49 (65.31%), Postives = 40/49 (81.63%), Query Frame = -3 
Query:  343 VEERDVVTFPGFVDCVYLDASNELQLDNGLGDLISIKNTNWSDSVFVEP 489
            VEER+VV FPGFVDC+YL A N+L L+NGLGD +SI+N NW+D+V   P
Sbjct:  272 VEEREVVNFPGFVDCIYLTAPNKLLLENGLGDTVSIENKNWADAVLWNP 320
[Close]
BLAST of HO066383 vs. TrEMBL
Match: C5X9W0_SORBI (Putative uncharacterized protein Sb02g022170 OS=Sorghum bicolor GN=Sb02g022170 PE=4 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 1.709e-27
Identity = 32/42 (76.19%), Postives = 36/42 (85.71%), Query Frame = -1 
Query:  228 LWNPHLQMEACYKDFVCVENAKIGSVQLEPEQTWTAVQHLSI 353
            LWNPHLQMEACYKDFVCVENAKI  VQLEP+Q+W A Q + +
Sbjct:  274 LWNPHLQMEACYKDFVCVENAKIEMVQLEPKQSWVAEQKIEL 315

HSP 2 Score: 73.1738 bits (178), Expect = 1.709e-27
Identity = 33/48 (68.75%), Postives = 39/48 (81.25%), Query Frame = -3 
Query:  343 EERDVVTFPGFVDCVYLDASNELQLDNGLGDLISIKNTNWSDSVFVEP 486
            EER+ VTFPGFVDC+YL A +EL LDNGLGD I+I N+NWSD+V   P
Sbjct:  230 EEREEVTFPGFVDCIYLGAPSELILDNGLGDKIAIANSNWSDAVLWNP 277
[Close]
BLAST of HO066383 vs. TrEMBL
Match: A9U353_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_155277 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 4.097e-22
Identity = 29/42 (69.05%), Postives = 36/42 (85.71%), Query Frame = -1 
Query:  228 LWNPHLQMEACYKDFVCVENAKIGSVQLEPEQTWTAVQHLSI 353
            LWNP+L MEACYKDFVCVENA++  V+L+P Q+WTA Q LS+
Sbjct:  274 LWNPYLTMEACYKDFVCVENAQLSPVELQPGQSWTAKQSLSL 315

HSP 2 Score: 60.4622 bits (145), Expect = 4.097e-22
Identity = 25/48 (52.08%), Postives = 36/48 (75.00%), Query Frame = -3 
Query:  343 EERDVVTFPGFVDCVYLDASNELQLDNGLGDLISIKNTNWSDSVFVEP 486
            E+R+V+ FPGFVD +Y++  N L LDNGLG+ +SI+N  W+D+V   P
Sbjct:  230 EDRNVIEFPGFVDTMYMNTPNVLVLDNGLGEKLSIRNEGWTDAVLWNP 277
[Close]
BLAST of HO066383 vs. TrEMBL
Match: B9S6E7_RICCO (Aldose 1-epimerase, putative OS=Ricinus communis GN=RCOM_0535930 PE=4 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 2.202e-15
Identity = 55/129 (42.64%), Postives = 73/129 (56.59%), Query Frame = -1 
Query:  228 NSGLAQHISGFCNVASLK-VKVAKTLNKDPDRXXXXXXXXXXGMWSLSQDLWIVFILT---LQMSCNLIMAWAI*YLSRTQTGQIRCLWNPHLQMEACYKDFVCVENAKIGSVQLEPEQTWTAVQHLSI 602
            N+ L  + S     AS++ +K  KTLNKDPD            + ++   +  +++     LQ+   L     I   + + T     LWNPHLQMEACYKDFVCVENAKIG V+LEP Q+WTA QH+SI
Sbjct:  188 NTALHTYFSASVTGASVRGLKGCKTLNKDPDPNNPIEGKEERDVVTVPGFVDCIYLDAPEELQLDNGLGDVITIKNTNWSDT----VLWNPHLQMEACYKDFVCVENAKIGKVELEPGQSWTAKQHISI 312
[Close]
BLAST of HO066383 vs. TrEMBL
Match: B8A1H0_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 6.630e-12
Identity = 52/131 (39.69%), Postives = 64/131 (48.85%), Query Frame = -1 
Query:  228 NSGLAQHISGFCNVASLK-VKVAKTLNKDPDRXXXXXXXXXXGMWSLSQDLWIVFI-----LTLQMSCNLIMAWAI*YLSRTQTGQIRCLWNPHLQMEACYKDFVCVENAKIGSVQLEPEQTWTAVQHLSI 602
            NS L  + S      S+K +K  KTLNKDPD              +    +  V++     L L       +A A              LWNPHLQMEACYKDFVCVENAKI  VQLEP+Q+W A Q + +
Sbjct:  190 NSALHTYFSASITGVSVKGLKGCKTLNKDPDPKNPLEGKEEREEVTFPGFVDCVYLGAPSELILDNGLGDKIAIA------NSNWSDAVLWNPHLQMEACYKDFVCVENAKIEMVQLEPKQSWVAEQKIEL 314
[Close]
BLAST of HO066383 vs. TAIR peptide
Match: AT5G66530.2 (| Symbols: | Galactose mutarotase-like superfamily protein | chr5:26553821-26555575 REVERSE LENGTH=307)

HSP 1 Score: 79.7221 bits (195), Expect = 2.476e-32
Identity = 37/48 (77.08%), Postives = 39/48 (81.25%), Query Frame = -3 
Query:  343 EERDVVTFPGFVDCVYLDASNELQLDNGLGDLISIKNTNWSDSVFVEP 486
            E+RD VTFPGFVD VYLDA NELQ DNGLGD I IKNTNWSD+V   P
Sbjct:  221 EDRDAVTFPGFVDTVYLDAPNELQFDNGLGDKIIIKNTNWSDAVLWNP 268

HSP 2 Score: 77.411 bits (189), Expect = 2.476e-32
Identity = 33/43 (76.74%), Postives = 38/43 (88.37%), Query Frame = -1 
Query:  225 LWNPHLQMEACYKDFVCVENAKIGSVQLEPEQTWTAVQHLSIA 353
            LWNPH QMEACY+DFVCVENAK+G V+LEP Q+WTA Q LSI+
Sbjct:  265 LWNPHTQMEACYRDFVCVENAKLGDVKLEPGQSWTATQLLSIS 307
[Close]
BLAST of HO066383 vs. TAIR peptide
Match: AT5G66530.1 (| Symbols: | Galactose mutarotase-like superfamily protein | chr5:26553821-26555575 REVERSE LENGTH=307)

HSP 1 Score: 79.7221 bits (195), Expect = 2.476e-32
Identity = 37/48 (77.08%), Postives = 39/48 (81.25%), Query Frame = -3 
Query:  343 EERDVVTFPGFVDCVYLDASNELQLDNGLGDLISIKNTNWSDSVFVEP 486
            E+RD VTFPGFVD VYLDA NELQ DNGLGD I IKNTNWSD+V   P
Sbjct:  221 EDRDAVTFPGFVDTVYLDAPNELQFDNGLGDKIIIKNTNWSDAVLWNP 268

HSP 2 Score: 77.411 bits (189), Expect = 2.476e-32
Identity = 33/43 (76.74%), Postives = 38/43 (88.37%), Query Frame = -1 
Query:  225 LWNPHLQMEACYKDFVCVENAKIGSVQLEPEQTWTAVQHLSIA 353
            LWNPH QMEACY+DFVCVENAK+G V+LEP Q+WTA Q LSI+
Sbjct:  265 LWNPHTQMEACYRDFVCVENAKLGDVKLEPGQSWTATQLLSIS 307
The following BLAST results are available for this feature:
BLAST of HO066383 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
C6TCM2_SOYBN1.263e-3897.67Putative uncharacterized protein OS=Glycine max PE... [more]
D7SPC0_VITVI2.925e-3477.08Whole genome shotgun sequence of line PN40024, sca... [more]
B9HF22_POPTR3.158e-3388.10Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
D7MMG0_ARALY5.771e-3283.33Aldose 1-epimerase family protein OS=Arabidopsis l... [more]
Q9FJY6_ARATH3.949e-3077.08Apospory-associated protein C-like OS=Arabidopsis ... [more]
A9NZI3_PICSI1.218e-2876.19Putative uncharacterized protein OS=Picea sitchens... [more]
C5X9W0_SORBI1.709e-2776.19Putative uncharacterized protein Sb02g022170 OS=So... [more]
A9U353_PHYPA4.097e-2269.05Predicted protein OS=Physcomitrella patens subsp. ... [more]
B9S6E7_RICCO2.202e-1542.64Aldose 1-epimerase, putative OS=Ricinus communis G... [more]
B8A1H0_MAIZE6.630e-1239.69Putative uncharacterized protein OS=Zea mays PE=2 ... [more]
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BLAST of HO066383 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT5G66530.22.476e-3277.08| Symbols: | Galactose mutarotase-like superfamil... [more]
AT5G66530.12.476e-3277.08| Symbols: | Galactose mutarotase-like superfamil... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014718Glycoside hydrolase-type carbohydrate-binding, subgroupGENE3D2.70.98.10no descriptioncoord: 18..71
score: 0.
IPR014718Glycoside hydrolase-type carbohydrate-binding, subgroupGENE3D2.70.98.10no descriptioncoord: 12..53
score: 2.4

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>HO066383 ID=HO066383; Name=HO066383; organism=Cicer arietinum; type=EST; length=842bp
GAAAAAATAGAAAATTATAATAACTTTATTTTGTTTGTTACAACAATGTT
ACATATGTAATGAGGATTGATTTTATGAGTTAACACCTCTTGCAAATTAT
CAAATCCCTCACCACCAGCCAACTATTAGTGGCTTAGTAAATTATTATAC
AAAAGATACATATTGTTGGCAAAATCAAAGAAGACAAAAAATAATTGATA
ATGTAGAACCACCGTAACTGTTCAAGCAATGGAAAGATGCTGCACAGCTG
TCCAAGTTTGTTCTGGTTCTAGCTGGACACTTCCAATCTTAGCATTTTCA
ACACAAACAAAATCTTTGTAACATGCTTCCATTTGAAGATGAGGGTTCCA
CAAACACCGAATCTGACCAGTTTGTGTTCTTGATAGATATTAGATCGCCC
AAGCCATTATCAAGTTGCAACTCATTTGAAGCGTCAAGATAAACACAATC
CACAAATCCTGGGAAAGTGACCACATCCCTTTCTTCGACTAACCTCCAAT
GGATTTTTAGCGATCTGGATCTTTGTTCAGAGTTTTTGCAACTTTAACCT
TTAATGATGCAACATTACAGAAGCCCGAAATATGTTGTGCAAGGCCCGAA
TTTGACATTGACAGAAATGTNCTTAATTTGGGACCCTGGGNATTTATATA
AAAGCGTTTTTTAGAACNTTACCTAGGTTCGGAAAGAAGCAACTTTTTTA
GCCATTNTAAAGTTGGTAGATACCTTGGTTAATTNAAGGGCATTGGGGAA
GNCCGGAAATTCCCCCATTAATGCCAGGGTATAAAAGGGATTAACACTTT
GTAATTTCCACAAAATTTTATTATCAGGGAATATTCCCCTAC
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0030246carbohydrate binding
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR014718GH-type_carb-bd