CK148811

EST Overview
NameCK148811
Unique NameCK148811
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length444
Libraries
Library NameType
LIBEST_014674 Suppressive subtractive hybridization librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
Homology
[Close]
BLAST of CK148811 vs. TrEMBL
Match: D7TWI8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_66.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00032403001 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 4.564e-32
Identity = 42/78 (53.85%), Postives = 58/78 (74.36%), Query Frame = 3 
Query:  102 KLDLPTTFYDNLGNPFYEACNSVHFYAETNYPDVLAINRTADGKGNVHIKAIRHGKTLVRVAISDAQQKSDYILIRAG 335
            ++ LP  F D LGNPF+EA N +   AETNYPD+++IN T DG GN+H+K IRHG+ L+RV+I+ +  KSDY+L+  G
Sbjct: 1491 EVKLPINFCDVLGNPFHEAFNVIPLDAETNYPDIVSINSTGDGYGNIHLKGIRHGRALLRVSINSSPHKSDYVLVSVG 1568

HSP 2 Score: 49.2914 bits (116), Expect = 4.564e-32
Identity = 20/33 (60.61%), Postives = 26/33 (78.79%), Query Frame = 2 
Query:  335 AHIYPQNPVLHIGSPFNLSIKGLNDKVSGQWFT 433
            A++ P+NPVLH+G   N SI+GL DKVSGQW +
Sbjct: 1569 AYLSPRNPVLHLGGHLNFSIEGLKDKVSGQWLS 1601

HSP 3 Score: 36.1946 bits (82), Expect = 4.564e-32
Identity = 17/33 (51.52%), Postives = 24/33 (72.73%), Query Frame = 1 
Query:   10 VASCIKVADVTQIRIGSKEVLLKVIDLAVGANL 108
            +ASC++VA+V QIRI  ++    VIDLAV A +
Sbjct: 1460 IASCVRVAEVAQIRITPQKFSFHVIDLAVDAEV 1492
[Close]
BLAST of CK148811 vs. TrEMBL
Match: B9S651_RICCO (RNA binding protein, putative OS=Ricinus communis GN=RCOM_1064510 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 3.263e-28
Identity = 40/78 (51.28%), Postives = 59/78 (75.64%), Query Frame = 3 
Query:  102 KLDLPTTFYDNLGNPFYEACNSVHFYAETNYPDVLAINRTADGKGNVHIKAIRHGKTLVRVAISDAQQKSDYILIRAG 335
            +LDL  +++D LGNPFYEA N+V ++AETNY D+++I+ T      +H+KA+R+G+ L+RV+  D QQKSD+ILI  G
Sbjct: 1469 ELDLSISYFDALGNPFYEAHNAVSYHAETNYHDIVSIDDTKTDSEKIHLKALRYGRALLRVSFKDNQQKSDFILISVG 1546

HSP 2 Score: 39.6614 bits (91), Expect = 3.263e-28
Identity = 19/36 (52.78%), Postives = 25/36 (69.44%), Query Frame = 1 
Query:    1 RAEVASCIKVADVTQIRIGSKEVLLKVIDLAVGANL 108
            R E+ASC++VA+V QIRI +KE    VI +AV   L
Sbjct: 1435 RVEIASCVRVAEVAQIRITNKEFPFHVIHVAVNTEL 1470

HSP 3 Score: 38.891 bits (89), Expect = 3.263e-28
Identity = 18/33 (54.55%), Postives = 24/33 (72.73%), Query Frame = 2 
Query:  335 AHIYPQNPVLHIGSPFNLSIKGLNDKVSGQWFT 433
            A+I+PQNPVLH GS  + SI+G   +VSG W +
Sbjct: 1547 ANIFPQNPVLHQGSSLHFSIEG--SQVSGHWLS 1577
[Close]
BLAST of CK148811 vs. TrEMBL
Match: Q9FI62_ARATH (Nuclear pore protein-like OS=Arabidopsis thaliana PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.442e-27
Identity = 35/78 (44.87%), Postives = 52/78 (66.67%), Query Frame = 3 
Query:  102 KLDLPTTFYDNLGNPFYEACNSVHFYAETNYPDVLAINRTADGKGNVHIKAIRHGKTLVRVAISDAQQKSDYILIRAG 335
            +L+LP  +YD LG PF EA     +  ETN+ DV+ I +T + + + +IK I+HGK L+RV+I D  +KSDY+L+  G
Sbjct: 1469 ELELPINYYDTLGIPFLEAHGVTTYNVETNHRDVVFI-KTVNDQPSAYIKGIKHGKALIRVSIGDNLRKSDYVLVSVG 1545

HSP 2 Score: 51.6026 bits (122), Expect = 3.442e-27
Identity = 21/36 (58.33%), Postives = 29/36 (80.56%), Query Frame = 2 
Query:  335 AHIYPQNPVLHIGSPFNLSIKGLNDKVSGQWFTT*R 442
            AHI+PQNPV+H G+  N SI G +++V+GQWFT+ R
Sbjct: 1546 AHIFPQNPVIHTGNLLNFSITGADNEVTGQWFTSNR 1581

HSP 3 Score: 41.5874 bits (96), Expect = 3.442e-27
Identity = 19/36 (52.78%), Postives = 27/36 (75.00%), Query Frame = 1 
Query:    1 RAEVASCIKVADVTQIRIGSKEVLLKVIDLAVGANL 108
            R E+A+C++VA+V QIR+ S+ +   VIDLAVG  L
Sbjct: 1435 RIEIAACVRVAEVAQIRMKSEGIPFHVIDLAVGGEL 1470
[Close]
BLAST of CK148811 vs. TrEMBL
Match: D7MJ71_ARALY (EMB3012 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_493939 PE=4 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.137e-26
Identity = 34/78 (43.59%), Postives = 53/78 (67.95%), Query Frame = 3 
Query:  102 KLDLPTTFYDNLGNPFYEACNSVHFYAETNYPDVLAINRTADGKGNVHIKAIRHGKTLVRVAISDAQQKSDYILIRAG 335
            +L+LP  +YD LG PF EA   + +  ETN+ DV++I +T + + + +IK I+HGK L+RV+I    +KSDY+L+  G
Sbjct: 1453 ELELPINYYDTLGIPFLEAHGVITYNVETNHRDVVSI-KTVNDQPSAYIKGIKHGKALIRVSIGGNLRKSDYVLVSVG 1529

HSP 2 Score: 49.2914 bits (116), Expect = 2.137e-26
Identity = 21/36 (58.33%), Postives = 27/36 (75.00%), Query Frame = 2 
Query:  335 AHIYPQNPVLHIGSPFNLSIKGLNDKVSGQWFTT*R 442
            AHI+PQNPV+H G+  N SI G + +VSGQW T+ R
Sbjct: 1530 AHIFPQNPVIHTGNVLNFSIAGSDHEVSGQWVTSNR 1565

HSP 3 Score: 41.5874 bits (96), Expect = 2.137e-26
Identity = 19/36 (52.78%), Postives = 27/36 (75.00%), Query Frame = 1 
Query:    1 RAEVASCIKVADVTQIRIGSKEVLLKVIDLAVGANL 108
            R E+A+C++VA+V QIR+ S+ +   VIDLAVG  L
Sbjct: 1419 RIEIAACVRVAEVAQIRMKSEGIPFHVIDLAVGGEL 1454
[Close]
BLAST of CK148811 vs. TrEMBL
Match: D7KAY4_ARALY (EMB3012 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_474789 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.723e-25
Identity = 35/79 (44.30%), Postives = 53/79 (67.09%), Query Frame = 3 
Query:  102 KLDLPTTFYDNLGNPFYEACNSVHFYAETNYPDVLAINRTADGKGNVHIKAIRHGKTLVRVAISDAQ-QKSDYILIRAG 335
            +L+LP  +YD LG PF EA   + + AETN+ DV+ I +T + + + +IK I+HGK L+RV+I     +KSDY+L+  G
Sbjct: 1454 ELELPINYYDTLGIPFLEAHGVITYNAETNHRDVVYI-KTVNDQPSAYIKGIKHGKALIRVSIGGKNLRKSDYVLVSVG 1531

HSP 2 Score: 49.2914 bits (116), Expect = 1.723e-25
Identity = 21/36 (58.33%), Postives = 27/36 (75.00%), Query Frame = 2 
Query:  335 AHIYPQNPVLHIGSPFNLSIKGLNDKVSGQWFTT*R 442
            AHI+PQNPV+H G+  N SI G + +VSGQW T+ R
Sbjct: 1532 AHIFPQNPVIHTGNLLNFSIAGADHEVSGQWVTSNR 1567

HSP 3 Score: 41.5874 bits (96), Expect = 1.723e-25
Identity = 19/36 (52.78%), Postives = 27/36 (75.00%), Query Frame = 1 
Query:    1 RAEVASCIKVADVTQIRIGSKEVLLKVIDLAVGANL 108
            R E+A+C++VA+V QIR+ S+ +   VIDLAVG  L
Sbjct: 1420 RIEIAACVRVAEVAQIRMKSEGIPFHVIDLAVGGEL 1455
[Close]
BLAST of CK148811 vs. TrEMBL
Match: B8BEL9_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_32422 PE=4 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 1.181e-8
Identity = 31/84 (36.90%), Postives = 46/84 (54.76%), Query Frame = 3 
Query:  102 KLDLPTTFYDNLGNPFYEACNSVHFYAETNYPDVLAINRTADGKG--NVH----IKAIRHGKTLVRVAISDAQQKSDYILIRAG 335
            K++L   + D LG  F EA   V    ETN+PDV++I    +G G    H    ++A  HG  LVR+ IS   +K+D+I++  G
Sbjct: 1447 KVELDIKYSDELGYTFSEALGIVPVKIETNHPDVVSILMPKEGNGTHGTHERFVLQARSHGTALVRLQISHIPKKADFIMVSVG 1530

HSP 2 Score: 30.4166 bits (67), Expect = 1.181e-8
Identity = 13/32 (40.62%), Postives = 22/32 (68.75%), Query Frame = 1 
Query:    1 RAEVASCIKVADVTQIRIGSKEVLLKVIDLAV 96
            R E+ASC++VA+V Q +I + E  + +  L+V
Sbjct: 1413 RTEIASCLRVAEVAQAQIAAAESSIHIAYLSV 1444
[Close]
BLAST of CK148811 vs. TrEMBL
Match: B9G551_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_30371 PE=4 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 1.182e-8
Identity = 31/84 (36.90%), Postives = 46/84 (54.76%), Query Frame = 3 
Query:  102 KLDLPTTFYDNLGNPFYEACNSVHFYAETNYPDVLAINRTADGKG--NVH----IKAIRHGKTLVRVAISDAQQKSDYILIRAG 335
            K++L   + D LG  F EA   V    ETN+PDV++I    +G G    H    ++A  HG  LVR+ IS   +K+D+I++  G
Sbjct: 1393 KVELDIKYSDELGYTFSEALGIVPVKIETNHPDVVSILMPKEGNGTHGTHERFVLQARSHGTALVRLQISHIPKKADFIMVSVG 1476

HSP 2 Score: 30.4166 bits (67), Expect = 1.182e-8
Identity = 13/32 (40.62%), Postives = 22/32 (68.75%), Query Frame = 1 
Query:    1 RAEVASCIKVADVTQIRIGSKEVLLKVIDLAV 96
            R E+ASC++VA+V Q +I + E  + +  L+V
Sbjct: 1359 RTEIASCLRVAEVAQAQIAAAESSIHIAYLSV 1390
[Close]
BLAST of CK148811 vs. TrEMBL
Match: Q650W3_ORYSJ (Os09g0565500 protein OS=Oryza sativa subsp. japonica GN=OSJNBa0070E11.8 PE=4 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 1.221e-8
Identity = 31/84 (36.90%), Postives = 46/84 (54.76%), Query Frame = 3 
Query:  102 KLDLPTTFYDNLGNPFYEACNSVHFYAETNYPDVLAINRTADGKG--NVH----IKAIRHGKTLVRVAISDAQQKSDYILIRAG 335
            K++L   + D LG  F EA   V    ETN+PDV++I    +G G    H    ++A  HG  LVR+ IS   +K+D+I++  G
Sbjct:  139 KVELDIKYSDELGYTFSEALGIVPVKIETNHPDVVSILMPKEGNGTHGTHERFVLQARSHGTALVRLQISHIPKKADFIMVSVG 222

HSP 2 Score: 30.4166 bits (67), Expect = 1.221e-8
Identity = 13/32 (40.62%), Postives = 22/32 (68.75%), Query Frame = 1 
Query:    1 RAEVASCIKVADVTQIRIGSKEVLLKVIDLAV 96
            R E+ASC++VA+V Q +I + E  + +  L+V
Sbjct:  105 RTEIASCLRVAEVAQAQIAAAESSIHIAYLSV 136
[Close]
BLAST of CK148811 vs. TrEMBL
Match: A9S7H8_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_163274 PE=4 SV=1)

HSP 1 Score: 45.8246 bits (107), Expect = 2.569e-8
Identity = 26/81 (32.10%), Postives = 39/81 (48.15%), Query Frame = 3 
Query:  111 LPTTFYDNLGNPFYEACNSVHFYAETNYPDVLAINRT------ADGKGNVHIKAIRHGKTLVRVAISDAQQKSDYILIRAG 335
            L  T  D+LG PF EA  ++    +TN  D++ +            K  + +KA+R G  LVRV   +  Q  D+I+I  G
Sbjct: 1561 LAVTLRDDLGVPFLEAGTTIPITLDTNRADLITVKVVDVEIVGRSSKATILVKAVRQGSALVRVTYKNNPQIVDWIMINVG 1641

HSP 2 Score: 35.8094 bits (81), Expect = 2.569e-8
Identity = 14/34 (41.18%), Postives = 25/34 (73.53%), Query Frame = 2 
Query:  335 AHIYPQNPVLHIGSPFNLSIKGLNDKVSGQWFTT 436
            A++YP++PVLHIG+  + SI G  ++  G W+++
Sbjct: 1642 AYVYPRSPVLHIGNRLSFSILGKGER--GSWYSS 1673
[Close]
BLAST of CK148811 vs. TAIR peptide
Match: AT5G40480.1 (| Symbols: EMB3012 | embryo defective 3012 | chr5:16213361-16223982 FORWARD LENGTH=1923)

HSP 1 Score: 72.4034 bits (176), Expect = 2.285e-29
Identity = 35/78 (44.87%), Postives = 52/78 (66.67%), Query Frame = 3 
Query:  102 KLDLPTTFYDNLGNPFYEACNSVHFYAETNYPDVLAINRTADGKGNVHIKAIRHGKTLVRVAISDAQQKSDYILIRAG 335
            +L+LP  +YD LG PF EA     +  ETN+ DV+ I +T + + + +IK I+HGK L+RV+I D  +KSDY+L+  G
Sbjct: 1457 ELELPINYYDTLGIPFLEAHGVTTYNVETNHRDVVFI-KTVNDQPSAYIKGIKHGKALIRVSIGDNLRKSDYVLVSVG 1533

HSP 2 Score: 51.6026 bits (122), Expect = 2.285e-29
Identity = 21/36 (58.33%), Postives = 29/36 (80.56%), Query Frame = 2 
Query:  335 AHIYPQNPVLHIGSPFNLSIKGLNDKVSGQWFTT*R 442
            AHI+PQNPV+H G+  N SI G +++V+GQWFT+ R
Sbjct: 1534 AHIFPQNPVIHTGNLLNFSITGADNEVTGQWFTSNR 1569

HSP 3 Score: 41.5874 bits (96), Expect = 2.285e-29
Identity = 19/36 (52.78%), Postives = 27/36 (75.00%), Query Frame = 1 
Query:    1 RAEVASCIKVADVTQIRIGSKEVLLKVIDLAVGANL 108
            R E+A+C++VA+V QIR+ S+ +   VIDLAVG  L
Sbjct: 1423 RIEIAACVRVAEVAQIRMKSEGIPFHVIDLAVGGEL 1458
The following BLAST results are available for this feature:
BLAST of CK148811 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 9
Match NameE-valueIdentityDescription
D7TWI8_VITVI4.564e-3253.85Whole genome shotgun sequence of line PN40024, sca... [more]
B9S651_RICCO3.263e-2851.28RNA binding protein, putative OS=Ricinus communis ... [more]
Q9FI62_ARATH3.442e-2744.87Nuclear pore protein-like OS=Arabidopsis thaliana ... [more]
D7MJ71_ARALY2.137e-2643.59EMB3012 OS=Arabidopsis lyrata subsp. lyrata GN=ARA... [more]
D7KAY4_ARALY1.723e-2544.30EMB3012 OS=Arabidopsis lyrata subsp. lyrata GN=ARA... [more]
B8BEL9_ORYSI1.181e-836.90Putative uncharacterized protein OS=Oryza sativa s... [more]
B9G551_ORYSJ1.182e-836.90Putative uncharacterized protein OS=Oryza sativa s... [more]
Q650W3_ORYSJ1.221e-836.90Os09g0565500 protein OS=Oryza sativa subsp. japoni... [more]
A9S7H8_PHYPA2.569e-832.10Predicted protein OS=Physcomitrella patens subsp. ... [more]
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BLAST of CK148811 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT5G40480.12.285e-2944.87| Symbols: EMB3012 | embryo defective 3012 | chr5:... [more]
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Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>CK148811 ID=CK148811; Name=CK148811; organism=Cicer arietinum; type=EST; length=444bp
CGAGCCGAGGTCGCTTCTTGCATCAAGGTTGCTGATGTGACTCAAATTAG
AATAGGCAGTAAGGAGGTTCTCCTCAAAGTGATTGACCTTGCTGTTGGTG
CAAACTTGATCTTCCAACAACCTTTTATGATAATCTAGGAAATCCTTTCT
ATGAAGCATGCAATTCAGTGCATTTCTACGCTGAAACTAACTACCCTGAT
GTTCTTGCCATCAACAGAACTGCTGATGGAAAAGGGAATGTCCACATCAA
GGCAATTCGGCACGGGAAAACTCTTGTGCGTGTAGCAATTAGTGATGCCC
AGCAAAAGTCAGATTACATACTGATTAGGGCTGGGCCCATATATATCCTC
AAAATCCGGTTCTTCATATAGGAAGTCCTTTTAACTTGAGCATAAAAGGA
TTGAATGATAAAGTTTCTGGACAGTGGTTTACCACCTGACGGAA
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