FL512381

EST Overview


Name	FL512381
Unique Name	FL512381
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	551

Libraries


Library Name	Type
LIBEST_023567 Subtracted cDNA library of drought-stressed chickpea cultivar	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of FL512381 vs. SwissProt
Match: CYSP_PEA (Cysteine proteinase 15A OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 1.201e-47
Identity = 88/96 (91.67%), Postives = 92/96 (95.83%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGNG-FAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTVPAA 287
            LVKNGPLAVAINAAWMQTYMSGVSCPY+CAKSRLDHGVLLVGFG G +APIRLKEKPYWIIKNSWGQNWGE+GYYKICRGRN+ GVDSMVSTV AA
Sbjct:  264 LVKNGPLAVAINAAWMQTYMSGVSCPYVCAKSRLDHGVLLVGFGKGAYAPIRLKEKPYWIIKNSWGQNWGEQGYYKICRGRNVCGVDSMVSTVAAA 359

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BLAST of FL512381 vs. SwissProt
Match: PAPA5_CARPA (Cysteine proteinase (Fragment) OS=Carica papaya PE=3 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 2.053e-39
Identity = 76/97 (78.35%), Postives = 86/97 (88.66%), Query Frame = 3

Query:   15 GPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTVPAASCIQQ 302
            GPLAVAINAA+MQTY+ GVSCPYIC++ RL+HGVLLVG+G+ G+APIRLKEKPYW+IKNSWG+NWGE GYYKICRGRNI GVDSMVSTV A     Q
Sbjct:    1 GPLAVAINAAYMQTYIGGVSCPYICSR-RLNHGVLLVGYGSAGYAPIRLKEKPYWVIKNSWGENWGENGYYKICRGRNICGVDSMVSTVAAVHTTSQ 96

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BLAST of FL512381 vs. SwissProt
Match: A494_ARATH (Probable cysteine proteinase A494 OS=Arabidopsis thaliana GN=At2g21430 PE=2 SV=2)

HSP 1 Score: 157.918 bits (398), Expect = 2.965e-38
Identity = 73/97 (75.26%), Postives = 88/97 (90.72%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTVPAAS 290
            L+KNGPLAVAINAA+MQTY+ GVSCPYIC++ RL+HGVLLVG+G+ GF+  RLKEKPYWIIKNSWG++WGE G+YKIC+GRNI GVDS+VSTV A +
Sbjct:  265 LIKNGPLAVAINAAYMQTYIGGVSCPYICSR-RLNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFYKICKGRNICGVDSLVSTVAATT 360

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BLAST of FL512381 vs. SwissProt
Match: RD19A_ARATH (Cysteine proteinase RD19a OS=Arabidopsis thaliana GN=RD19A PE=2 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 5.057e-38
Identity = 74/95 (77.89%), Postives = 83/95 (87.37%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTVPA 284
            LVKNGPLAVAINA +MQTY+ GVSCPYIC + RL+HGVLLVG+G  G+AP R KEKPYWIIKNSWG+ WGE G+YKIC+GRNI GVDSMVSTV A
Sbjct:  268 LVKNGPLAVAINAGYMQTYIGGVSCPYICTR-RLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICGVDSMVSTVAA 361

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BLAST of FL512381 vs. SwissProt
Match: CYSP1_MAIZE (Cysteine proteinase 1 OS=Zea mays GN=CCP1 PE=2 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 1.127e-37
Identity = 75/98 (76.53%), Postives = 85/98 (86.73%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFG-NGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRG---RNIWGVDSMVSTVPA 284
            L+K+GPLA+ INAA+MQTY+ GVSCPYIC +  LDHGVLLVG+G +GFAPIRLK+KPYWIIKNSWG+NWGE GYYKICRG   RN  GVDSMVSTV A
Sbjct:  269 LIKHGPLAIGINAAYMQTYIGGVSCPYICGR-HLDHGVLLVGYGASGFAPIRLKDKPYWIIKNSWGENWGENGYYKICRGSNVRNKCGVDSMVSTVSA 365

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BLAST of FL512381 vs. SwissProt
Match: CPR1_DROME (Putative cysteine proteinase CG12163 OS=Drosophila melanogaster GN=CG12163 PE=2 SV=2)

HSP 1 Score: 104.76 bits (260), Expect = 2.979e-22
Identity = 49/97 (50.52%), Postives = 64/97 (65.98%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPY--ICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTVPAA 287
            L+ NGP+++ INA  MQ Y  GVS P+  +C+K  LDHGVL+VG+G    P   K  PYWI+KNSWG  WGE+GYY++ RG N  GV  M ++   A
Sbjct:  518 LLANGPISIGINANAMQFYRGGVSHPWKALCSKKNLDHGVLVVGYGVSDYPNFHKTLPYWIVKNSWGPRWGEQGYYRVYRGDNTCGVSEMATSAVLA 614

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BLAST of FL512381 vs. SwissProt
Match: CATF_MOUSE (Cathepsin F OS=Mus musculus GN=Ctsf PE=2 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 1.998e-18
Identity = 45/93 (48.39%), Postives = 60/93 (64.52%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPY--ICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVST 275
            L + GP++VAINA  MQ Y  G++ P+  +C+   +DH VLLVG+GN          PYW IKNSWG +WGEEGYY + RG    GV++M S+
Sbjct:  372 LAQKGPISVAINAFGMQFYRHGIAHPFRPLCSPWFIDHAVLLVGYGNR------SNIPYWAIKNSWGSDWGEEGYYYLYRGSGACGVNTMASS 458

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BLAST of FL512381 vs. SwissProt
Match: CATF_HUMAN (Cathepsin F OS=Homo sapiens GN=CTSF PE=1 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 4.451e-18
Identity = 45/93 (48.39%), Postives = 60/93 (64.52%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPY--ICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVST 275
            L K GP++VAINA  MQ Y  G+S P   +C+   +DH VLLVG+GN        + P+W IKNSWG +WGE+GYY + RG    GV++M S+
Sbjct:  394 LAKRGPISVAINAFGMQFYRHGISRPLRPLCSPWLIDHAVLLVGYGNR------SDVPFWAIKNSWGTDWGEKGYYYLHRGSGACGVNTMASS 480

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BLAST of FL512381 vs. SwissProt
Match: CYSP1_DICDI (Cysteine proteinase 1 OS=Dictyostelium discoideum GN=cprA PE=1 SV=2)

HSP 1 Score: 86.2705 bits (212), Expect = 1.096e-16
Identity = 43/91 (47.25%), Postives = 57/91 (62.64%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVST 275
            +V  GPLA+A +A   Q Y+ GV     C  + LDHG+L+VG+       R K  PYWI+KNSWG +WGE+GY  + RG+N  GV + VST
Sbjct:  252 IVSTGPLAIAADAVEWQFYIGGVF-DIPCNPNSLDHGILIVGYSAKNTIFR-KNMPYWIVKNSWGADWGEQGYIYLRRGKNTCGVSNFVST 340

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BLAST of FL512381 vs. SwissProt
Match: CATL_SCHMA (Cathepsin L OS=Schistosoma mansoni GN=CL1 PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 9.281e-16
Identity = 38/93 (40.86%), Postives = 59/93 (63.44%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPY--ICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVST 275
            L  N  ++V +NA  +Q Y  G+S P+   C+K  LDH VLLVG+G     +  K +P+WI+KNSWG  WGE GY+++ RG    G++++ ++
Sbjct:  228 LYHNSTISVGMNALLLQFYQHGISHPWWIFCSKYLLDHAVLLVGYG-----VSEKNEPFWIVKNSWGVEWGENGYFRMYRGDGSCGINTVATS 315

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BLAST of FL512381 vs. TrEMBL
Match: O81930_CICAR (Cysteine proteinase OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 197.593 bits (501), Expect = 4.552e-49
Identity = 92/94 (97.87%), Postives = 92/94 (97.87%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTVPA 284
            LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNI GVDSMVSTV A
Sbjct:  263 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNICGVDSMVSTVAA 356

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BLAST of FL512381 vs. TrEMBL
Match: Q9STA4_MEDSA (Cysteine protease (Fragment) OS=Medicago sativa GN=cyp15a PE=2 SV=1)

HSP 1 Score: 187.963 bits (476), Expect = 3.607e-46
Identity = 88/96 (91.67%), Postives = 93/96 (96.88%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGNG-FAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTVPAA 287
            LVKNGPLAVAINAAWMQTYMSGVSCP+ICAK+RLDHGVLLVGFG+G +APIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRN+ GVDSMVSTV AA
Sbjct:  112 LVKNGPLAVAINAAWMQTYMSGVSCPHICAKARLDHGVLLVGFGSGGYAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNVCGVDSMVSTVAAA 207

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BLAST of FL512381 vs. TrEMBL
Match: Q8GV06_VICFA (Pre-pro cysteine proteinase OS=Vicia faba PE=3 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 6.153e-46
Identity = 86/96 (89.58%), Postives = 91/96 (94.79%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGNG-FAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTVPAA 287
            LVKNGPLAV INAAWMQTYMSGVSCPY+CAKSRLDHGVLLVGFG G +APIRLKEKPYWI+KNSWGQNWGE+GYYKICRGRN+ GVDSMVSTV AA
Sbjct:  264 LVKNGPLAVGINAAWMQTYMSGVSCPYVCAKSRLDHGVLLVGFGKGAYAPIRLKEKPYWIVKNSWGQNWGEQGYYKICRGRNVCGVDSMVSTVAAA 359

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BLAST of FL512381 vs. TrEMBL
Match: Q41671_VICFA (Pre-pro-cysteine proteinase OS=Vicia faba GN=VFCYSPRO PE=2 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 6.153e-46
Identity = 86/96 (89.58%), Postives = 91/96 (94.79%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGNG-FAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTVPAA 287
            LVKNGPLAV INAAWMQTYMSGVSCPY+CAKSRLDHGVLLVGFG G +APIRLKEKPYWI+KNSWGQNWGE+GYYKICRGRN+ GVDSMVSTV AA
Sbjct:  264 LVKNGPLAVGINAAWMQTYMSGVSCPYVCAKSRLDHGVLLVGFGKGAYAPIRLKEKPYWIVKNSWGQNWGEQGYYKICRGRNVCGVDSMVSTVAAA 359

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BLAST of FL512381 vs. TrEMBL
Match: Q41698_VICSA (Cysteine proteinase OS=Vicia sativa PE=2 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.371e-45
Identity = 86/96 (89.58%), Postives = 91/96 (94.79%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGNG-FAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTVPAA 287
            LVKNGPLAVAINAAWMQ YMSGVSCPY+CAK+RLDHGVLLVGFG G +APIRLKEKPYWIIKNSWGQNWGE+GYYKICRGRN+ GVDSMVSTV AA
Sbjct:  259 LVKNGPLAVAINAAWMQAYMSGVSCPYVCAKARLDHGVLLVGFGKGAYAPIRLKEKPYWIIKNSWGQNWGEQGYYKICRGRNVCGVDSMVSTVAAA 354

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BLAST of FL512381 vs. TrEMBL
Match: Q5Y804_PETHY (Cysteine proteinase (Fragment) OS=Petunia hybrida GN=CP5 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 3.494e-41
Identity = 79/96 (82.29%), Postives = 87/96 (90.62%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTVPAAS 290
            LVKNGPLAVAINA +MQTY+ GVSCPYIC+K R DHGVLLVG+G+GFAPIR+KEKPYWIIKNSWG+ WGE GYYKICRGRN+ GVDSMVSTV A S
Sbjct:   93 LVKNGPLAVAINAVFMQTYVGGVSCPYICSK-RQDHGVLLVGYGSGFAPIRMKEKPYWIIKNSWGEKWGESGYYKICRGRNVCGVDSMVSTVAAVS 187

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BLAST of FL512381 vs. TrEMBL
Match: A2PZE0_IPONI (Cysteine proteinase OS=Ipomoea nil GN=In15 PE=2 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 1.328e-40
Identity = 77/101 (76.24%), Postives = 89/101 (88.12%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTVPAASCIQQ 302
            LVK+GPL+V INA +MQTY+ GVSCPYIC+K  LDHGVLLVG+G  G+APIR K+KPYWI+KNSWG+NWGEEGYYKICRG NI G+DSMVSTV AAS I+Q
Sbjct:  269 LVKHGPLSVGINAVFMQTYIGGVSCPYICSKRNLDHGVLLVGYGAAGYAPIRFKDKPYWIVKNSWGENWGEEGYYKICRGNNICGIDSMVSTVTAASTIKQ 369

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BLAST of FL512381 vs. TrEMBL
Match: B9RHA4_RICCO (Cysteine protease, putative OS=Ricinus communis GN=RCOM_1449120 PE=3 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 1.734e-40
Identity = 80/95 (84.21%), Postives = 88/95 (92.63%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTVPA 284
            LVKNGPLAVAINA +MQTY+ GVSCPYIC+K RLDHGVLLVG+G+ G+APIR+KEKPYWIIKNSWG+NWGE GYYKICRGRNI GVDSMVSTV A
Sbjct:  274 LVKNGPLAVAINAVFMQTYIGGVSCPYICSK-RLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGENWGESGYYKICRGRNICGVDSMVSTVAA 367

[Close]

BLAST of FL512381 vs. TrEMBL
Match: Q9M7D6_IPOBA (Papain-like cysteine proteinase isoform I OS=Ipomoea batatas GN=IBPCPI PE=2 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 2.958e-40
Identity = 79/97 (81.44%), Postives = 89/97 (91.75%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTVPAAS 290
            LVKNGPLAVAINA +MQTY+ GVSCPYIC+K RLDHGVLLVG+G+ G+APIR+KEKPYWIIKNSWG++WGE GYYKICRGRN+ GVDSMVSTV A S
Sbjct:  268 LVKNGPLAVAINAVFMQTYIGGVSCPYICSK-RLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGESWGENGYYKICRGRNVCGVDSMVSTVAAVS 363

[Close]

BLAST of FL512381 vs. TrEMBL
Match: Q9M7D4_IPOBA (Papain-like cysteine proteinase isoform III OS=Ipomoea batatas GN=IBPCPIII PE=2 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 2.958e-40
Identity = 79/97 (81.44%), Postives = 89/97 (91.75%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTVPAAS 290
            LVKNGPLAVAINA +MQTY+ GVSCPYIC+K RLDHGVLLVG+G+ G+APIR+KEKPYWIIKNSWG++WGE GYYKICRGRN+ GVDSMVSTV A S
Sbjct:  266 LVKNGPLAVAINAVFMQTYIGGVSCPYICSK-RLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGESWGENGYYKICRGRNVCGVDSMVSTVAAVS 361

[Close]

BLAST of FL512381 vs. TAIR peptide
Match: AT2G21430.1 (| Symbols: | Papain family cysteine protease | chr2:9171964-9173301 REVERSE LENGTH=361)

HSP 1 Score: 157.918 bits (398), Expect = 3.197e-39
Identity = 73/97 (75.26%), Postives = 88/97 (90.72%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTVPAAS 290
            L+KNGPLAVAINAA+MQTY+ GVSCPYIC++ RL+HGVLLVG+G+ GF+  RLKEKPYWIIKNSWG++WGE G+YKIC+GRNI GVDS+VSTV A +
Sbjct:  265 LIKNGPLAVAINAAYMQTYIGGVSCPYICSR-RLNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFYKICKGRNICGVDSLVSTVAATT 360

[Close]

BLAST of FL512381 vs. TAIR peptide
Match: AT4G39090.1 (| Symbols: RD19, RD19A | Papain family cysteine protease | chr4:18215826-18217326 REVERSE LENGTH=368)

HSP 1 Score: 157.147 bits (396), Expect = 5.453e-39
Identity = 74/95 (77.89%), Postives = 83/95 (87.37%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTVPA 284
            LVKNGPLAVAINA +MQTY+ GVSCPYIC + RL+HGVLLVG+G  G+AP R KEKPYWIIKNSWG+ WGE G+YKIC+GRNI GVDSMVSTV A
Sbjct:  268 LVKNGPLAVAINAGYMQTYIGGVSCPYICTR-RLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICGVDSMVSTVAA 361

[Close]

BLAST of FL512381 vs. TAIR peptide
Match: AT4G16190.1 (| Symbols: | Papain family cysteine protease | chr4:9171512-9172877 FORWARD LENGTH=373)

HSP 1 Score: 154.451 bits (389), Expect = 3.535e-38
Identity = 73/96 (76.04%), Postives = 85/96 (88.54%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRG-RNIWGVDSMVSTVPA 284
            LV++GPLA+AINA WMQTY+ GVSCPY+C+KS+ DHGVLLVGFG+ G+APIRLKEKPYWIIKNSWG  WGE GYYKICRG  N+ G+D+MVSTV A
Sbjct:  273 LVQHGPLAIAINAMWMQTYIGGVSCPYVCSKSQ-DHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGEHGYYKICRGPHNMCGMDTMVSTVAA 367

[Close]

BLAST of FL512381 vs. TAIR peptide
Match: AT3G54940.2 (| Symbols: | Papain family cysteine protease | chr3:20354402-20356127 FORWARD LENGTH=367)

HSP 1 Score: 139.428 bits (350), Expect = 1.177e-33
Identity = 61/93 (65.59%), Postives = 78/93 (83.87%), Query Frame = 3

Query:    3 LVKNGPLAVAINAAWMQTYMSGVSCPYICAKSRLDHGVLLVGFGN-GFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVSTV 278
            LV++GPLAV +NA +MQTY+ GVSCP IC+K  ++HGVLLVG+G+ GF+ +RL  KPYWIIKNSWG+ WGE GYYK+CRG +I G++SMVS V
Sbjct:  269 LVRHGPLAVGLNAVFMQTYIGGVSCPLICSKRNVNHGVLLVGYGSKGFSILRLSNKPYWIIKNSWGKKWGENGYYKLCRGHDICGINSMVSAV 361

[Close]

BLAST of FL512381 vs. TAIR peptide
Match: AT3G19390.1 (| Symbols: | Granulin repeat cysteine protease family protein | chr3:6723024-6724768 FORWARD LENGTH=452)

HSP 1 Score: 63.5438 bits (153), Expect = 8.205e-11
Identity = 36/77 (46.75%), Postives = 46/77 (59.74%), Query Frame = 3

Query:   12 NGPLAVAINAAW--MQTYMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICR 236
            N P++VAI A     Q Y SGV     C  S LDHGV+ VG+G+         + YWI++NSWG NWGE GY+K+ R
Sbjct:  258 NQPISVAIEAGGRAFQLYTSGVFTG-TCGTS-LDHGVVAVGYGSEGG------QDYWIVRNSWGSNWGESGYFKLER 326

[Close]

BLAST of FL512381 vs. TAIR peptide
Match: AT5G43060.1 (| Symbols: | Granulin repeat cysteine protease family protein | chr5:17269784-17272117 REVERSE LENGTH=463)

HSP 1 Score: 61.2326 bits (147), Expect = 4.072e-10
Identity = 36/77 (46.75%), Postives = 45/77 (58.44%), Query Frame = 3

Query:   18 PLAVAINAAW--MQTYMSGVSCPYICAKSRLDHGVLLVGFG--NGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICR 236
            P++VAI A     Q Y SGV    +C  + LDHGV+ VG+G  NG        K YWI++NSWG  WGE GY K+ R
Sbjct:  268 PISVAIEAGGRAFQLYSSGVF-DGLCG-TELDHGVVAVGYGTENG--------KDYWIVRNSWGNRWGESGYIKMAR 334

[Close]

BLAST of FL512381 vs. TAIR peptide
Match: AT5G60360.1 (| Symbols: SAG2, AALP, ALP | aleurain-like protease | chr5:24280044-24282152 FORWARD LENGTH=358)

HSP 1 Score: 61.2326 bits (147), Expect = 4.072e-10
Identity = 33/90 (36.67%), Postives = 51/90 (56.67%), Query Frame = 3

Query:   18 PLAVAINAAW-MQTYMSGVSCPYICAKSRLD--HGVLLVGFG--NGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGVDSMVS 272
            P+++A       + Y SGV     C  + +D  H VL VG+G  +G         PYW+IKNSWG +WG++GY+K+  G+N+ G+ +  S
Sbjct:  272 PVSIAFEVIHSFRLYKSGVYTDSHCGSTPMDVNHAVLAVGYGVEDGV--------PYWLIKNSWGADWGDKGYFKMEMGKNMCGIATCAS 353

[Close]

BLAST of FL512381 vs. TAIR peptide
Match: AT3G19400.1 (| Symbols: | Cysteine proteinases superfamily protein | chr3:6725510-6726878 FORWARD LENGTH=362)

HSP 1 Score: 60.8474 bits (146), Expect = 5.319e-10
Identity = 36/79 (45.57%), Postives = 48/79 (60.76%), Query Frame = 3

Query:    6 VKNGPLAVAINAAWM--QTYMSGVSCPYICAKSRLDHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICR 236
            V + P++VAI A+    Q Y SGV     C  S LDHGV++VG+G+         + YWII+NSWG NWG+ GY K+ R
Sbjct:  259 VAHQPVSVAIEASSQAFQLYKSGVMTG-TCGIS-LDHGVVVVGYGS------TSGEDYWIIRNSWGLNWGDSGYVKLQR 329

[Close]

BLAST of FL512381 vs. TAIR peptide
Match: AT3G45310.1 (| Symbols: | Cysteine proteinases superfamily protein | chr3:16628704-16630473 REVERSE LENGTH=358)

HSP 1 Score: 60.4622 bits (145), Expect = 6.946e-10
Identity = 32/83 (38.55%), Postives = 45/83 (54.22%), Query Frame = 3

Query:   18 PLAVAINAAW-MQTYMSGVSCPYICAKSRLD--HGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICRGRNIWGV 257
            P++VA       + Y  GV     C  + +D  H VL VG+G         + PYW+IKNSWG  WG+ GY+K+  G+N+ GV
Sbjct:  272 PVSVAFEVVHEFRFYKKGVFTSNTCGNTPMDVNHAVLAVGYGVE------DDVPYWLIKNSWGGEWGDNGYFKMEMGKNMCGV 348

[Close]

BLAST of FL512381 vs. TAIR peptide
Match: AT3G43960.1 (| Symbols: | Cysteine proteinases superfamily protein | chr3:15774122-15775628 REVERSE LENGTH=376)

HSP 1 Score: 60.4622 bits (145), Expect = 6.946e-10
Identity = 35/75 (46.67%), Postives = 44/75 (58.67%), Query Frame = 3

Query:   18 PLAVAINAAWMQTYMSGVSCPYICAKSRL--DHGVLLVGFGNGFAPIRLKEKPYWIIKNSWGQNWGEEGYYKICR 236
            P++V I+AA M  Y SGV   Y  A S L  DH VL+VG+G         E  YW+I+NSWG  WGE GY ++ R
Sbjct:  261 PISVMISAANMSDYKSGV---YKGACSNLWGDHNVLIVGYGTSS-----DEGDYWLIRNSWGPEWGEGGYLRLQR 327

The following BLAST results are available for this feature:

BLAST of FL512381 vs. SwissProt
BLAST of FL512381 vs. TrEMBL
BLAST of FL512381 vs. TAIR peptide

BLAST of FL512381 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
CYSP_PEA	1.201e-47	91.67	Cysteine proteinase 15A OS=Pisum sativum PE=2 SV=1	[more]
PAPA5_CARPA	2.053e-39	78.35	Cysteine proteinase (Fragment) OS=Carica papaya PE...	[more]
A494_ARATH	2.965e-38	75.26	Probable cysteine proteinase A494 OS=Arabidopsis t...	[more]
RD19A_ARATH	5.057e-38	77.89	Cysteine proteinase RD19a OS=Arabidopsis thaliana ...	[more]
CYSP1_MAIZE	1.127e-37	76.53	Cysteine proteinase 1 OS=Zea mays GN=CCP1 PE=2 SV=...	[more]
CPR1_DROME	2.979e-22	50.52	Putative cysteine proteinase CG12163 OS=Drosophila...	[more]
CATF_MOUSE	1.998e-18	48.39	Cathepsin F OS=Mus musculus GN=Ctsf PE=2 SV=1	[more]
CATF_HUMAN	4.451e-18	48.39	Cathepsin F OS=Homo sapiens GN=CTSF PE=1 SV=1	[more]
CYSP1_DICDI	1.096e-16	47.25	Cysteine proteinase 1 OS=Dictyostelium discoideum ...	[more]
CATL_SCHMA	9.281e-16	40.86	Cathepsin L OS=Schistosoma mansoni GN=CL1 PE=2 SV=...	[more]

BLAST of FL512381 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
O81930_CICAR	4.552e-49	97.87	Cysteine proteinase OS=Cicer arietinum PE=2 SV=1	[more]
Q9STA4_MEDSA	3.607e-46	91.67	Cysteine protease (Fragment) OS=Medicago sativa GN...	[more]
Q8GV06_VICFA	6.153e-46	89.58	Pre-pro cysteine proteinase OS=Vicia faba PE=3 SV=...	[more]
Q41671_VICFA	6.153e-46	89.58	Pre-pro-cysteine proteinase OS=Vicia faba GN=VFCYS...	[more]
Q41698_VICSA	1.371e-45	89.58	Cysteine proteinase OS=Vicia sativa PE=2 SV=1	[more]
Q5Y804_PETHY	3.494e-41	82.29	Cysteine proteinase (Fragment) OS=Petunia hybrida ...	[more]
A2PZE0_IPONI	1.328e-40	76.24	Cysteine proteinase OS=Ipomoea nil GN=In15 PE=2 SV...	[more]
B9RHA4_RICCO	1.734e-40	84.21	Cysteine protease, putative OS=Ricinus communis GN...	[more]
Q9M7D6_IPOBA	2.958e-40	81.44	Papain-like cysteine proteinase isoform I OS=Ipomo...	[more]
Q9M7D4_IPOBA	2.958e-40	81.44	Papain-like cysteine proteinase isoform III OS=Ipo...	[more]

BLAST of FL512381 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT2G21430.1	3.197e-39	75.26	\| Symbols: \| Papain family cysteine protease \| ch...	[more]
AT4G39090.1	5.453e-39	77.89	\| Symbols: RD19, RD19A \| Papain family cysteine pr...	[more]
AT4G16190.1	3.535e-38	76.04	\| Symbols: \| Papain family cysteine protease \| ch...	[more]
AT3G54940.2	1.177e-33	65.59	\| Symbols: \| Papain family cysteine protease \| ch...	[more]
AT3G19390.1	8.205e-11	46.75	\| Symbols: \| Granulin repeat cysteine protease fa...	[more]
AT5G43060.1	4.072e-10	46.75	\| Symbols: \| Granulin repeat cysteine protease fa...	[more]
AT5G60360.1	4.072e-10	36.67	\| Symbols: SAG2, AALP, ALP \| aleurain-like proteas...	[more]
AT3G19400.1	5.319e-10	45.57	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT3G45310.1	6.946e-10	38.55	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT3G43960.1	6.946e-10	46.67	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 22..35 score
None	No IPR available	GENE3D	3.90.70.10	no description	coord: 2..92 score: 1.1
None	No IPR available	PANTHER	PTHR12411:SF42	CYSTEINE PROTEASE	coord: 1..94 score: 6.3
None	No IPR available	SUPERFAMILY	54001	Cysteine proteinases	coord: 1..94 score: 2
IPR000169	Peptidase, cysteine peptidase active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 34..44 score
IPR000169	Peptidase, cysteine peptidase active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 57..76 score
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 2..91 score: 7.6
IPR013128	Peptidase C1A, papain	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 1..94 score: 6.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FL512381 ID=FL512381; Name=FL512381; organism=Cicer arietinum; type=EST; length=551bp
ATTTAGTAAAGAATGGCCCTCTTGCAGTTGCTATTAACGCAGCCTGGATG
CAAACATATATGAGTGGTGTCTCATGCCCATACATCTGTGCAAAATCGCG
TTTGGATCATGGGGTTCTTCTAGTTGGTTTTGGAAATGGTTTTGCTCCCA
TCCGATTGAAGGAAAAGCCTTACTGGATCATAAAGAATTCATGGGGACAG
AATTGGGGTGAGGAAGGATATTACAAGATCTGCAGAGGTCGAAATATATG
GGGAGTGGATTCAATGGTCTCAACTGTACCTGCAGCTTCATGCATCCAAC
AATTAATAATACGGAGATGCTGGTTCTGGTGGTCTCATCCTATGTGAAAC
GCCCTCTTAGTTCAATTTGTGTTAGATATATAATCATGAAGGTGCTTGAA
TTTAATTTCTGGTTCATGGCAAGGACTAAACTCCCTTTTCCGGATCAGCA
TAACTACCCACCAAACTAGGTAATTAATCTTAATTGGGAATTTATGCCAT
GCCTTGTCACCCAGAACCGTCGCTCTACCCCCACAGTGCAAGAGTAACAT
A

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004197	cysteine-type endopeptidase activity
GO:0008234	cysteine-type peptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **INTERPRO**
Term	Definition
IPR000169	Pept_cys_AS
IPR000668	Peptidase_C1A_C
IPR013128	Peptidase_C1A

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