GR402373

EST Overview


Name	GR402373
Unique Name	GR402373
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	687

Libraries


Library Name	Type
LIBEST_024785 ICCV2 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR402373 vs. SwissProt
Match: CML21_ARATH (Probable calcium-binding protein CML21 OS=Arabidopsis thaliana GN=CML21 PE=2 SV=1)

HSP 1 Score: 257.684 bits (657), Expect = 4.315e-68
Identity = 122/177 (68.93%), Postives = 148/177 (83.62%), Query Frame = 1

Query:  154 MGGALGKDESPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINET 684
            MGGA+ K E+ +K W PETKLEAK++EA+QRR   G ++KSFN+I+LKFPKID+  R C+AIF++FDEDSNG+ID  ELK C  KLEISF EEEINDLF+ACDIN+D+G+ F EFIVLLCLVYLLKDD + L  K  +GMP LE TFETLVDTFVFLD+NKDGYVS+ EMV+AI+E+
Sbjct:    1 MGGAVTKSETLQKEWVPETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCKAIFQEFDEDSNGSIDHTELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLCLVYLLKDDSSTLQKKWTMGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDES 177

[Close]

BLAST of GR402373 vs. SwissProt
Match: CML22_ARATH (Probable calcium-binding protein CML22 OS=Arabidopsis thaliana GN=CML22 PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 2.023e-33
Identity = 72/168 (42.86%), Postives = 113/168 (67.26%), Query Frame = 1

Query:  178 ESPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSR-IGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAIN 678
            E+ KK    + KL  KMVE+      H  S+KS ++II+KFPK+ E  R  R++FE +D D+NG ID EELKKC  +L++S S+EE+  L+  CD++   G++FNEFIVLLCL+YLL    +    +SR +G   +E+ F+ +V+ F+FLDK+  G ++K+++++ +N
Sbjct:    9 ENNKKYAELDAKLARKMVESRSIYPGHR-SLKSMDSIIMKFPKLREGLRNIRSVFESYDNDTNGTIDIEELKKCLEELKLSLSDEEVKGLYSWCDVDGSKGIQFNEFIVLLCLIYLLAKPSSESSTESREMGPKLVESIFDPIVEVFLFLDKDGKGKLNKADVIKTLN 175

[Close]

BLAST of GR402373 vs. SwissProt
Match: CALM_YEAST (Calmodulin OS=Saccharomyces cerevisiae GN=CMD1 PE=1 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 1.907e-7
Identity = 33/118 (27.97%), Postives = 55/118 (46.61%), Query Frame = 1

Query:  310 DESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEM 663
            +E   + +  F  FD+D+NG+I   EL      L +S SE E+NDL    D++ +  ++F+EF+ L+       D                    + L++ F   DKN DG +S +E+
Sbjct:    7 EEQIAEFKEAFALFDKDNNGSISSSELATVMRSLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSRQLKSNDSE------------------QELLEAFKVFDKNGDGLISAAEL 106

[Close]

BLAST of GR402373 vs. SwissProt
Match: CDPK6_ARATH (Calcium-dependent protein kinase 6 OS=Arabidopsis thaliana GN=CPK6 PE=1 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 3.252e-7
Identity = 44/167 (26.35%), Postives = 73/167 (43.71%), Query Frame = 1

Query:  181 SPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINE 681
            +P ++  P      K   AM +       +K     ++     +E     RA+FE  D D++GAI  +ELK    +   +  + EI DL EA D+++   + ++EFI             A +H      +  LE   E LV  F + DK+  GY++  E+ Q+  E
Sbjct:  349 APDRALDPAVLSRLKQFSAMNK-------LKKMALKVIAESLSEEEIAGLRAMFEAMDTDNSGAITFDELKAGLRRYGSTLKDTEIRDLMEAADVDNSGTIDYSEFIA------------ATIH------LNKLERE-EHLVSAFQYFDKDGSGYITIDELQQSCIE 489

[Close]

BLAST of GR402373 vs. SwissProt
Match: LPS1A_LYTPI (Calcium-binding protein LPS1-alpha OS=Lytechinus pictus PE=2 SV=2)

HSP 1 Score: 54.299 bits (129), Expect = 7.245e-7
Identity = 41/141 (29.08%), Postives = 71/141 (50.35%), Query Frame = 1

Query:  277 FNTIILKFPK-----IDESFRKC-RAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINE 681
            F+T+++K P       DE +R+  +  FE+FD++ +G++   E+ +  SK    +S++EI  L    D+NDD  ++FNEF + L       D  +  H K +                F+ +DK+K+G +S  EMV  I +
Sbjct:  147 FDTLVVKLPIGMGPCKDEEYREYYKNEFEKFDKNGDGSLTTAEMSEFMSK-STKYSDKEIEYLISRVDLNDDGRVQFNEFFMHL-------DGVSKDHIKQQ----------------FMAIDKDKNGKISPEEMVFGITK 263

[Close]

BLAST of GR402373 vs. TrEMBL
Match: C6THI8_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 317.005 bits (811), Expect = 8.893e-85
Identity = 158/178 (88.76%), Postives = 165/178 (92.70%), Query Frame = 1

Query:  154 MGGALGKDESPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINETT 687
            MGG LGK ESP+K   PETKLEAKMVEAMQRRE  G SVKSFNTIILKFPKIDES RKC+AIFE FD DSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDIN+D+GMKFNEFIVLLC+VYLLK+DPAALHAKSRIGMP LE TFETLVDTFVFLDKNKDGYVSK+EMVQAINETT
Sbjct:    1 MGGTLGKSESPRKGSMPETKLEAKMVEAMQRRESQGSSVKSFNTIILKFPKIDESLRKCKAIFEPFDGDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINEDMGMKFNEFIVLLCVVYLLKNDPAALHAKSRIGMPKLEGTFETLVDTFVFLDKNKDGYVSKNEMVQAINETT 178

[Close]

BLAST of GR402373 vs. TrEMBL
Match: D7UAN9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_15.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00014761001 PE=4 SV=1)

HSP 1 Score: 309.301 bits (791), Expect = 1.854e-82
Identity = 150/178 (84.27%), Postives = 164/178 (92.13%), Query Frame = 1

Query:  154 MGGALGKDESPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINETT 687
            MGG +GK +SP+K+W PETKLEAKMVEAMQRR   G ++KSFN+IILKFPKIDES R C+AIF+QFDEDSNGAID EELK+CF KLEISFSEEEINDLFEACDIN+D+GMKFNEFIVLLCLVYLLK+DPAAL AKSR+GMPNLEATFETLVD FVFLDKNKDGYVSKSEMV AINETT
Sbjct:    1 MGGVVGKGDSPRKAWVPETKLEAKMVEAMQRRASEGSTMKSFNSIILKFPKIDESLRNCKAIFQQFDEDSNGAIDHEELKRCFLKLEISFSEEEINDLFEACDINEDMGMKFNEFIVLLCLVYLLKEDPAALQAKSRMGMPNLEATFETLVDAFVFLDKNKDGYVSKSEMVHAINETT 178

[Close]

BLAST of GR402373 vs. TrEMBL
Match: A5AMZ2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_006244 PE=4 SV=1)

HSP 1 Score: 302.368 bits (773), Expect = 2.267e-80
Identity = 149/181 (82.32%), Postives = 163/181 (90.06%), Query Frame = 1

Query:  154 MGGALGKDESPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHA---KSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINETT 687
            MGG +GK +SP+K+W PETKLEAKMVEAMQRR   G ++KSFN+IILKFPKIDES R C+AIF+QFDEDSNGAID EELK+CF KLEISFSEEEINDLFEACDIN+D+GMKFNEFIVLLCLVYLLK+DP AL A   KSR+GMPNLEATFETLVD FVFLDKNKDGYVSKSEMV AINETT
Sbjct:    1 MGGVVGKGDSPRKAWVPETKLEAKMVEAMQRRASEGSTMKSFNSIILKFPKIDESLRNCKAIFQQFDEDSNGAIDHEELKRCFLKLEISFSEEEINDLFEACDINEDMGMKFNEFIVLLCLVYLLKEDPTALQAVSKKSRMGMPNLEATFETLVDAFVFLDKNKDGYVSKSEMVHAINETT 181

[Close]

BLAST of GR402373 vs. TrEMBL
Match: B9T3W8_RICCO (Troponin C, skeletal muscle, putative OS=Ricinus communis GN=RCOM_0169840 PE=4 SV=1)

HSP 1 Score: 300.442 bits (768), Expect = 8.614e-80
Identity = 147/177 (83.05%), Postives = 163/177 (92.09%), Query Frame = 1

Query:  154 MGGALGKDESPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINET 684
            MGG +GK +SP   +TPETKLEAKMVEAM+RR   GCS+KSFN+IILKFPKIDES RKC+AIFEQFDEDSNG IDQEEL+KCF KLEISF+EEE NDLFEACDIN+D+GMKFNEFIVLLCLVYLLKDD AA+HAKS++GMPNLEATFETLVDTF FLDKNKDGYVSK+EMVQAINE+
Sbjct:    1 MGGVVGKADSP--IYTPETKLEAKMVEAMRRRGAEGCSIKSFNSIILKFPKIDESLRKCKAIFEQFDEDSNGTIDQEELRKCFCKLEISFTEEETNDLFEACDINEDMGMKFNEFIVLLCLVYLLKDDSAAVHAKSKMGMPNLEATFETLVDTFAFLDKNKDGYVSKNEMVQAINES 175

[Close]

BLAST of GR402373 vs. TrEMBL
Match: B9GHJ5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_829970 PE=4 SV=1)

HSP 1 Score: 297.745 bits (761), Expect = 5.583e-79
Identity = 142/177 (80.23%), Postives = 162/177 (91.53%), Query Frame = 1

Query:  154 MGGALGKDESPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINET 684
            MGGA+GK  SP+K+W PETK+E K+VEAM+RRE HG S+KSFN+IILKFPKIDESFRKC+A FEQFDEDSNG+ID+EEL+KCF KLE +F++EEINDLFE CD+N+D+GMKFNEFIVLLCLVYLLKDDPA   AKSRIGMP+LEATFETLV  FVFLDKNKDGYVSKSEMVQAINE+
Sbjct:    1 MGGAVGKAGSPQKAWIPETKIETKVVEAMRRRETHGSSIKSFNSIILKFPKIDESFRKCKATFEQFDEDSNGSIDKEELRKCFHKLETAFTDEEINDLFETCDVNEDMGMKFNEFIVLLCLVYLLKDDPAIPQAKSRIGMPDLEATFETLVGAFVFLDKNKDGYVSKSEMVQAINES 177

[Close]

BLAST of GR402373 vs. TrEMBL
Match: C5Z5R5_SORBI (Putative uncharacterized protein Sb10g023890 OS=Sorghum bicolor GN=Sb10g023890 PE=4 SV=1)

HSP 1 Score: 262.307 bits (669), Expect = 2.599e-68
Identity = 130/178 (73.03%), Postives = 155/178 (87.08%), Query Frame = 1

Query:  154 MGGALGKDESPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINETT 687
            MG  LG+ ++ K+S+   +KLE+KMV+AMQ+R  HG S+KSF++II+KFPKIDESFRKC+ IFEQFDEDSNG ID+EELK CF KLEISF+EEEI+DLFEACDIN+D+GMKFNEFIV LCLVYLL ++PA   AK ++G+ NLEATFETLVD FVFLDKNKDGYVSK EM+QAINETT
Sbjct:    1 MGAVLGRHDTHKRSFHG-SKLESKMVDAMQKRALHGTSLKSFDSIIMKFPKIDESFRKCKTIFEQFDEDSNGEIDKEELKHCFQKLEISFTEEEISDLFEACDINEDMGMKFNEFIVFLCLVYLL-NEPAVSEAKIKMGLGNLEATFETLVDAFVFLDKNKDGYVSKDEMIQAINETT 176

[Close]

BLAST of GR402373 vs. TrEMBL
Match: C0JAF1_9ORYZ (Calcium ion binding protein OS=Oryza granulata PE=4 SV=1)

HSP 1 Score: 259.61 bits (662), Expect = 1.685e-67
Identity = 129/177 (72.88%), Postives = 154/177 (87.01%), Query Frame = 1

Query:  154 MGGALGKDESPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINET 684
            MGG LG+ ++ K+S +  +KLE KMV+A+Q+R  HG S+KSF++II+KFPKIDES RKC+AIFEQFDEDSNG ID++ELK CF KLEISF+EEEINDLFEACDIN+D+GMKFNEFIV LCL+YLL ++PA   AK ++G+ NLEATFETLVD FVFLDKNKDGYVSK EMVQAINET
Sbjct:    1 MGGILGRHDTLKRS-SHGSKLETKMVQAIQQRALHGTSLKSFDSIIMKFPKIDESLRKCKAIFEQFDEDSNGEIDKQELKHCFQKLEISFTEEEINDLFEACDINEDMGMKFNEFIVFLCLIYLL-NEPAVSEAKIKMGLGNLEATFETLVDAFVFLDKNKDGYVSKEEMVQAINET 175

[Close]

BLAST of GR402373 vs. TrEMBL
Match: B4FGG7_MAIZE (Calcium ion binding protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 259.225 bits (661), Expect = 2.201e-67
Identity = 130/178 (73.03%), Postives = 153/178 (85.96%), Query Frame = 1

Query:  154 MGGALGKDESPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINETT 687
            MG  LG+ ++ K+S +  +KLE KMV+AMQ+R  HG S+KSF++II+KFPKIDESFRKC+ IFEQFDEDSNG ID+EELK CF KLEISF+EEEI DLFEACDIN+D+GMKFNEFIV LCLVYLL ++ A L AK ++G+ NLEATFETLVD FVFLDKNKDGYVSK EM+QAINETT
Sbjct:    1 MGAVLGRHDTHKRS-SHGSKLEVKMVDAMQQRASHGTSLKSFDSIIMKFPKIDESFRKCKTIFEQFDEDSNGEIDKEELKHCFQKLEISFTEEEICDLFEACDINEDMGMKFNEFIVFLCLVYLL-NEAAVLEAKIKMGLGNLEATFETLVDAFVFLDKNKDGYVSKDEMIQAINETT 176

[Close]

BLAST of GR402373 vs. TrEMBL
Match: D7MF94_ARALY (Calcium ion binding protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_492166 PE=4 SV=1)

HSP 1 Score: 258.07 bits (658), Expect = 4.902e-67
Identity = 122/177 (68.93%), Postives = 148/177 (83.62%), Query Frame = 1

Query:  154 MGGALGKDESPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINET 684
            MGGA+ K E+ +K W PETKLEAK++EA+QRR   G ++KSFN+I+LKFPKID+  R C+AIF++FDEDSNG+ID  ELK C  KLEISF EEEINDLF+ACDIN+D+G+ F EFIVLLCLVYLLKDD + L  K  +GMP LE TFETLVDTFVFLD+NKDGYVS+ EMV+AI+E+
Sbjct:    1 MGGAVTKSETLQKEWVPETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCKAIFQEFDEDSNGSIDHTELKNCIKKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLCLVYLLKDDSSTLQKKWTMGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDES 177

[Close]

BLAST of GR402373 vs. TrEMBL
Match: Q69V45_ORYSJ (Calcium-binding EF hand-like protein OS=Oryza sativa subsp. japonica GN=P0556B08.33 PE=2 SV=1)

HSP 1 Score: 257.299 bits (656), Expect = 8.362e-67
Identity = 127/177 (71.75%), Postives = 153/177 (86.44%), Query Frame = 1

Query:  154 MGGALGKDESPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINET 684
            MGG LG+ ++ K+S +  +KLE KMVE+MQ+R  HG S+KSF++II+KFPKIDES RKC+ IFEQFDEDSNG ID++ELK CF KLEISF+EEEINDLFEACDIN+D+GMKFNEFIV LCL+YLL ++PA   AK ++G+ NLEATFETLVD FVFLDKNKDGYVSK EMVQ++NET
Sbjct:    1 MGGILGRHDTMKRS-SHGSKLETKMVESMQQRASHGTSLKSFDSIIMKFPKIDESLRKCKIIFEQFDEDSNGEIDKQELKHCFQKLEISFTEEEINDLFEACDINEDMGMKFNEFIVFLCLIYLL-NEPAVSEAKIKMGLGNLEATFETLVDAFVFLDKNKDGYVSKEEMVQSMNET 175

[Close]

BLAST of GR402373 vs. TAIR peptide
Match: AT4G26470.2 (| Symbols: | Calcium-binding EF-hand family protein | chr4:13371377-13372197 FORWARD LENGTH=211)

HSP 1 Score: 257.684 bits (657), Expect = 4.355e-69
Identity = 122/177 (68.93%), Postives = 148/177 (83.62%), Query Frame = 1

Query:  154 MGGALGKDESPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINET 684
            MGGA+ K E+ +K W PETKLEAK++EA+QRR   G ++KSFN+I+LKFPKID+  R C+AIF++FDEDSNG+ID  ELK C  KLEISF EEEINDLF+ACDIN+D+G+ F EFIVLLCLVYLLKDD + L  K  +GMP LE TFETLVDTFVFLD+NKDGYVS+ EMV+AI+E+
Sbjct:    1 MGGAVTKSETLQKEWVPETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCKAIFQEFDEDSNGSIDHTELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLCLVYLLKDDSSTLQKKWTMGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDES 177

[Close]

BLAST of GR402373 vs. TAIR peptide
Match: AT4G26470.1 (| Symbols: | Calcium-binding EF-hand family protein | chr4:13371377-13372343 FORWARD LENGTH=231)

HSP 1 Score: 257.684 bits (657), Expect = 4.355e-69
Identity = 122/177 (68.93%), Postives = 148/177 (83.62%), Query Frame = 1

Query:  154 MGGALGKDESPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINET 684
            MGGA+ K E+ +K W PETKLEAK++EA+QRR   G ++KSFN+I+LKFPKID+  R C+AIF++FDEDSNG+ID  ELK C  KLEISF EEEINDLF+ACDIN+D+G+ F EFIVLLCLVYLLKDD + L  K  +GMP LE TFETLVDTFVFLD+NKDGYVS+ EMV+AI+E+
Sbjct:    1 MGGAVTKSETLQKEWVPETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCKAIFQEFDEDSNGSIDHTELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLCLVYLLKDDSSTLQKKWTMGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDES 177

[Close]

BLAST of GR402373 vs. TAIR peptide
Match: AT3G24110.1 (| Symbols: | Calcium-binding EF-hand family protein | chr3:8704429-8705413 REVERSE LENGTH=229)

HSP 1 Score: 142.51 bits (358), Expect = 2.042e-34
Identity = 72/168 (42.86%), Postives = 113/168 (67.26%), Query Frame = 1

Query:  178 ESPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSR-IGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAIN 678
            E+ KK    + KL  KMVE+      H  S+KS ++II+KFPK+ E  R  R++FE +D D+NG ID EELKKC  +L++S S+EE+  L+  CD++   G++FNEFIVLLCL+YLL    +    +SR +G   +E+ F+ +V+ F+FLDK+  G ++K+++++ +N
Sbjct:    9 ENNKKYAELDAKLARKMVESRSIYPGHR-SLKSMDSIIMKFPKLREGLRNIRSVFESYDNDTNGTIDIEELKKCLEELKLSLSDEEVKGLYSWCDVDGSKGIQFNEFIVLLCLIYLLAKPSSESSTESREMGPKLVESIFDPIVEVFLFLDKDGKGKLNKADVIKTLN 175

[Close]

BLAST of GR402373 vs. TAIR peptide
Match: AT2G17290.1 (| Symbols: CPK6, ATCDPK3, ATCPK6 | Calcium-dependent protein kinase family protein | chr2:7517005-7519239 FORWARD LENGTH=544)

HSP 1 Score: 55.4546 bits (132), Expect = 3.283e-8
Identity = 44/167 (26.35%), Postives = 73/167 (43.71%), Query Frame = 1

Query:  181 SPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINE 681
            +P ++  P      K   AM +       +K     ++     +E     RA+FE  D D++GAI  +ELK    +   +  + EI DL EA D+++   + ++EFI             A +H      +  LE   E LV  F + DK+  GY++  E+ Q+  E
Sbjct:  349 APDRALDPAVLSRLKQFSAMNK-------LKKMALKVIAESLSEEEIAGLRAMFEAMDTDNSGAITFDELKAGLRRYGSTLKDTEIRDLMEAADVDNSGTIDYSEFIA------------ATIH------LNKLERE-EHLVSAFQYFDKDGSGYITIDELQQSCIE 489

[Close]

BLAST of GR402373 vs. TAIR peptide
Match: AT4G04700.1 (| Symbols: CPK27 | calcium-dependent protein kinase 27 | chr4:2385276-2387986 REVERSE LENGTH=485)

HSP 1 Score: 53.5286 bits (127), Expect = 1.247e-7
Identity = 37/138 (26.81%), Postives = 61/138 (44.20%), Query Frame = 1

Query:  277 FNTIILKFPKI---DESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINE 681
            F  ++LKF      +E  +  + +F   D D +G I  EELK   ++L  + S+ E+  L EA D++ +  +  +EFI      Y L  D                   E +   F   DK+ DG+++K E+  A+ E
Sbjct:  317 FKKVVLKFIAANLSEEEIKGLKTLFTNIDTDKSGNITLEELKTGLTRLGSNLSKTEVEQLMEAADMDGNGTIDIDEFISATMHRYKLDRD-------------------EHVYKAFQHFDKDNDGHITKEELEMAMKE 435

[Close]

BLAST of GR402373 vs. TAIR peptide
Match: AT3G20410.1 (| Symbols: CPK9 | calmodulin-domain protein kinase 9 | chr3:7116388-7118824 FORWARD LENGTH=541)

HSP 1 Score: 52.7582 bits (125), Expect = 2.128e-7
Identity = 35/123 (28.46%), Postives = 57/123 (46.34%), Query Frame = 1

Query:  313 ESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINE 681
            E  +  +A+F   D D++G I  EELK+  +KL    +E E+  L +A D++ +  + + EFI             A +H         LE+  E L   F   DK+  GY++  E+  A+ E
Sbjct:  392 EEIQGLKAMFANIDTDNSGTITYEELKEGLAKLGSKLTEAEVKQLMDAADVDGNGSIDYIEFIT------------ATMHRH------RLESN-ENLYKAFQHFDKDSSGYITIDELESALKE 495

[Close]

BLAST of GR402373 vs. TAIR peptide
Match: AT4G04695.1 (| Symbols: CPK31 | calcium-dependent protein kinase 31 | chr4:2381634-2383996 REVERSE LENGTH=484)

HSP 1 Score: 51.6026 bits (122), Expect = 4.740e-7
Identity = 32/124 (25.81%), Postives = 55/124 (44.35%), Query Frame = 1

Query:  310 DESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINE 681
            +E  +  + +F   D D +G I  EELK   ++L  + S+ E+  L EA D++ +  +  +EFI      Y L  D                   + +   F   DK+ DG+++K E+  A+ E
Sbjct:  331 EEEIKGLKTLFTNIDTDKSGTITLEELKTGLTRLGSNLSKTEVEQLMEAADVDGNGTIDIDEFISATMHRYRLDRD-------------------DHVYQAFQHFDKDNDGHITKEELEMAMKE 435

[Close]

BLAST of GR402373 vs. TAIR peptide
Match: AT4G35310.1 (| Symbols: CPK5, ATCPK5 | calmodulin-domain protein kinase 5 | chr4:16802436-16804628 FORWARD LENGTH=556)

HSP 1 Score: 51.6026 bits (122), Expect = 4.740e-7
Identity = 42/167 (25.15%), Postives = 73/167 (43.71%), Query Frame = 1

Query:  181 SPKKSWTPETKLEAKMVEAMQRRECHGCSVKSFNTIILKFPKIDESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINE 681
            +P ++  P      K   AM +       +K     ++     +E     R +F+  D D++GAI  +ELK    K   +  + EI+DL +A D+++   + ++EFI             A +H      +  LE   E LV  F + DK+  G+++  E+ QA  E
Sbjct:  361 APDRALDPAVLSRLKQFSAMNK-------LKKMALKVIAESLSEEEIAGLREMFQAMDTDNSGAITFDELKAGLRKYGSTLKDTEIHDLMDAADVDNSGTIDYSEFIA------------ATIH------LNKLERE-EHLVAAFQYFDKDGSGFITIDELQQACVE 501

[Close]

BLAST of GR402373 vs. TAIR peptide
Match: AT5G23580.1 (| Symbols: CDPK9, ATCDPK9, CPK12, ATCPK12 | calmodulin-like domain protein kinase 9 | chr5:7950388-7952433 REVERSE LENGTH=490)

HSP 1 Score: 51.2174 bits (121), Expect = 6.191e-7
Identity = 35/124 (28.23%), Postives = 55/124 (44.35%), Query Frame = 1

Query:  310 DESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINE 681
            +E     + +F+  D D +G I  EELK    ++     E EI +L  A D+++   + + EF+             A +H      +  LE   E LV  F F DK+  GY++  E+ QA  E
Sbjct:  322 EEEIGGLKELFKMIDTDKSGTITFEELKDSMRRVGSELMESEIQELLRAADVDESGTIDYGEFLA------------ATIH------LNKLERE-ENLVAAFSFFDKDASGYITIEELQQAWKE 426

[Close]

BLAST of GR402373 vs. TAIR peptide
Match: AT4G09570.1 (| Symbols: CPK4, ATCPK4 | calcium-dependent protein kinase 4 | chr4:6049560-6052184 FORWARD LENGTH=501)

HSP 1 Score: 50.8322 bits (120), Expect = 8.085e-7
Identity = 35/124 (28.23%), Postives = 54/124 (43.55%), Query Frame = 1

Query:  310 DESFRKCRAIFEQFDEDSNGAIDQEELKKCFSKLEISFSEEEINDLFEACDINDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKSEMVQAINE 681
            +E     + +F+  D D++G I  EELK    ++     E EI  L +A DI++   + + EF+             A LH        N     E LV  F + DK+  GY++  E+ QA  E
Sbjct:  325 EEEIGGLKELFKMIDTDNSGTITFEELKAGLKRVGSELMESEIKSLMDAADIDNSGTIDYGEFLA------------ATLHI-------NKMEREENLVVAFSYFDKDGSGYITIDELQQACTE 429

The following BLAST results are available for this feature:

BLAST of GR402373 vs. SwissProt
BLAST of GR402373 vs. TrEMBL
BLAST of GR402373 vs. TAIR peptide

BLAST of GR402373 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 5


Match Name	E-value	Identity	Description
CML21_ARATH	4.315e-68	68.93	Probable calcium-binding protein CML21 OS=Arabidop...	[more]
CML22_ARATH	2.023e-33	42.86	Probable calcium-binding protein CML22 OS=Arabidop...	[more]
CALM_YEAST	1.907e-7	27.97	Calmodulin OS=Saccharomyces cerevisiae GN=CMD1 PE=...	[more]
CDPK6_ARATH	3.252e-7	26.35	Calcium-dependent protein kinase 6 OS=Arabidopsis ...	[more]
LPS1A_LYTPI	7.245e-7	29.08	Calcium-binding protein LPS1-alpha OS=Lytechinus p...	[more]

BLAST of GR402373 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6THI8_SOYBN	8.893e-85	88.76	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7UAN9_VITVI	1.854e-82	84.27	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5AMZ2_VITVI	2.267e-80	82.32	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9T3W8_RICCO	8.614e-80	83.05	Troponin C, skeletal muscle, putative OS=Ricinus c...	[more]
B9GHJ5_POPTR	5.583e-79	80.23	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
C5Z5R5_SORBI	2.599e-68	73.03	Putative uncharacterized protein Sb10g023890 OS=So...	[more]
C0JAF1_9ORYZ	1.685e-67	72.88	Calcium ion binding protein OS=Oryza granulata PE=...	[more]
B4FGG7_MAIZE	2.201e-67	73.03	Calcium ion binding protein OS=Zea mays PE=2 SV=1	[more]
D7MF94_ARALY	4.902e-67	68.93	Calcium ion binding protein OS=Arabidopsis lyrata ...	[more]
Q69V45_ORYSJ	8.362e-67	71.75	Calcium-binding EF hand-like protein OS=Oryza sati...	[more]

BLAST of GR402373 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT4G26470.2	4.355e-69	68.93	\| Symbols: \| Calcium-binding EF-hand family prote...	[more]
AT4G26470.1	4.355e-69	68.93	\| Symbols: \| Calcium-binding EF-hand family prote...	[more]
AT3G24110.1	2.042e-34	42.86	\| Symbols: \| Calcium-binding EF-hand family prote...	[more]
AT2G17290.1	3.283e-8	26.35	\| Symbols: CPK6, ATCDPK3, ATCPK6 \| Calcium-depende...	[more]
AT4G04700.1	1.247e-7	26.81	\| Symbols: CPK27 \| calcium-dependent protein kinas...	[more]
AT3G20410.1	2.128e-7	28.46	\| Symbols: CPK9 \| calmodulin-domain protein kinase...	[more]
AT4G04695.1	4.740e-7	25.81	\| Symbols: CPK31 \| calcium-dependent protein kinas...	[more]
AT4G35310.1	4.740e-7	25.15	\| Symbols: CPK5, ATCPK5 \| calmodulin-domain protei...	[more]
AT5G23580.1	6.191e-7	28.23	\| Symbols: CDPK9, ATCDPK9, CPK12, ATCPK12 \| calmod...	[more]
AT4G09570.1	8.085e-7	28.23	\| Symbols: CPK4, ATCPK4 \| calcium-dependent protei...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	Calcium-binding EF-hand	SMART	SM00054	no description	coord: 60..88 score: 0.00053coord: 96..124 score: 48coord: 151..179 score: 0
IPR011992	EF-hand-like domain	GENE3D	1.10.238.10	no description	coord: 58..178 score: 8.3
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 69..81 score: NAcoord: 160..172 score
IPR018248	EF-hand	PFAM	PF00036	efhand	coord: 61..88 score: 3.7
IPR018249	EF-HAND 2	PROFILE	PS50222	EF_HAND_2	coord: 56..91 score: 13.063coord: 92..127 score: 7.540coord: 147..180 score: 10
None	No IPR available	PANTHER	PTHR10891	CALMODULIN	coord: 55..178 score: 1.2
None	No IPR available	PANTHER	PTHR10891:SF8	UNCHARACTERIZED	coord: 55..178 score: 1.2
None	No IPR available	SUPERFAMILY	47473	EF-hand	coord: 40..178 score: 1.5

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR402373 ID=GR402373; Name=GR402373; organism=Cicer arietinum; type=EST; length=687bp
GACACTAATTAATTATCATTATTATGCTTTCTTCTTCAACACCCACATTT
CTCTTTTCAGATTCAACAACTTTCACTTCTACTTTGCTTCCATCTCCACC
TCTTAGGTACTAGGGTATCATTTCTAAAGCATACTTTGGTTGATTGATTG
GCAATGGGAGGTGCATTAGGAAAGGATGAATCACCCAAAAAGTCTTGGAC
ACCAGAAACCAAGCTTGAGGCTAAAATGGTTGAAGCAATGCAACGGAGGG
AATGTCACGGATGTTCAGTGAAATCATTCAACACTATAATTCTAAAATTC
CCAAAAATTGATGAGAGCTTCAGAAAATGCAGAGCCATCTTTGAGCAGTT
TGATGAAGATTCTAATGGGGCAATAGATCAAGAAGAGTTGAAGAAGTGTT
TCAGTAAGCTGGAAATTTCATTTTCTGAGGAAGAGATCAATGATCTTTTT
GAAGCATGTGATATTAATGATGATTTGGGAATGAAGTTCAATGAGTTTAT
TGTACTTCTTTGCCTTGTCTATCTTCTCAAGGATGACCCTGCAGCTCTTC
ATGCTAAATCACGAATTGGCATGCCAAATCTAGAGGCCACATTCGAGACA
TTGGTCGATACATTTGTATTCTTAGACAAGAATAAGGATGGATATGTCAG
CAAAAGTGAAATGGTCCAAGCTATAAATGAAACTACA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS
IPR018248	EF-hand
IPR018249	EF-HAND 2

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