HO066418

EST Overview


Name	HO066418
Unique Name	HO066418
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	699

Libraries


Library Name	Type
LIBEST_026413 Chickpea drought stressed cDNA SSH library AS2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO066418 vs. SwissProt
Match: EOMT1_OCIBA (Eugenol O-methyltransferase OS=Ocimum basilicum GN=EOMT1 PE=1 SV=1)

HSP 1 Score: 47.3654 bits (111), Expect = 2.637e-7
Identity = 20/32 (62.50%), Postives = 23/32 (71.88%), Query Frame = -2

Query:  189 YDLVMLTMFLGKEREKKEWEKLIYDAGFSSYK 284
            +D+ M+  F  KER   EWEKLIYDAGF SYK
Sbjct:  311 FDMAMMCYFNAKERTMSEWEKLIYDAGFKSYK 342

HSP 2 Score: 28.1054 bits (61), Expect = 2.637e-7
Identity = 11/28 (39.29%), Postives = 19/28 (67.86%), Query Frame = -1

Query:  382 ADAVLLKWVLHGWNDEQS*RY*RTSKEA 465
            ADA+LLK ++H W+D +  +  +  K+A
Sbjct:  254 ADAILLKSIIHDWDDVEGLKILKKCKDA 281

[Close]

BLAST of HO066418 vs. SwissProt
Match: CVMT1_OCIBA (Chavicol O-methyltransferase OS=Ocimum basilicum GN=CVOMT1 PE=1 SV=1)

HSP 1 Score: 41.9726 bits (97), Expect = 5.734e-7
Identity = 18/32 (56.25%), Postives = 23/32 (71.88%), Query Frame = -2

Query:  189 YDLVMLTMFLGKEREKKEWEKLIYDAGFSSYK 284
            +D+ M++ F  KER   EWEKLI  AGF+SYK
Sbjct:  310 FDMAMMSYFNAKERTMNEWEKLISAAGFTSYK 341

HSP 2 Score: 32.3426 bits (72), Expect = 5.734e-7
Identity = 12/28 (42.86%), Postives = 21/28 (75.00%), Query Frame = -1

Query:  382 ADAVLLKWVLHGWNDEQS*RY*RTSKEA 465
            ADA+LLK+++H W+DE+  +  +  K+A
Sbjct:  253 ADAILLKFIIHDWDDEEGLKILKRCKDA 280

[Close]

BLAST of HO066418 vs. TrEMBL
Match: Q29U70_MEDTR (SAM dependent isoflavanone 4'-O-methyltransferase/(+)-6a-hydroxymaackiain-3-0-methyltransferase OS=Medicago truncatula PE=1 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.345e-11
Identity = 59/144 (40.97%), Postives = 70/144 (48.61%), Query Frame = -1

Query:  142 LKGTVFYQPQCCCVTXLXLKI*NFVDWRYVSSLFLLADAVLLKWVLHGWNDEQS*RY*RTSKEAXXXXXXXXXXXXXXXXINETSDDRELTGLQLDI*FGDADNVPWKRKRKERMGETYLRCWFQ*LQKLEP*DGFKSIIEVFP 573
            LK TVF QPQ          + NFV      S+   ADAVLLKWVLH WNDEQS +  + SKEA                I+ETSDDR LT LQLD           K + K+   +      F    K+ P  GFKS+IEV+P
Sbjct:  224 LKCTVFDQPQVVGNLTGNENL-NFVGGDMFKSI-PSADAVLLKWVLHDWNDEQSLKILKNSKEAISHKGKDGKVIIIDISIDETSDDRGLTELQLDYDLVMLTMFLGKERTKQEWEKLIYDAGFS-SYKITPISGFKSLIEVYP 364

[Close]

BLAST of HO066418 vs. TrEMBL
Match: Q84KK4_LOTJA (S-adenosyl-L-methionine: 2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase OS=Lotus japonicus GN=HI4'OMT PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.942e-10
Identity = 57/144 (39.58%), Postives = 69/144 (47.92%), Query Frame = -1

Query:  142 LKGTVFYQPQCCCVTXLXLKI*NFVDWRYVSSLFLLADAVLLKWVLHGWNDEQS*RY*RTSKEAXXXXXXXXXXXXXXXXINETSDDRELTGLQLDI*FGDADNVPWKRKRKERMGETYLRCWFQ*LQKLEP*DGFKSIIEVFP 573
            +K TVF QPQ          + NFV      S+   ADAVLLKWVLH WNDE S +  +  KEA                I+ETSDDRELT L+LD           K + K+   +      F    K+ P  GFKS+IEVFP
Sbjct:  225 VKCTVFDQPQVVANLTGDENL-NFVGGDMFKSV-PPADAVLLKWVLHDWNDELSLKILKNCKEAISGRGKEGKVIIIDISIDETSDDRELTELKLDYDLVMLTMFNGKEREKKEWEKLIYDAGFS-SYKITPICGFKSLIEVFP 365

[Close]

BLAST of HO066418 vs. TrEMBL
Match: Q84KK6_GLYEC (S-adenosyl-L-methionine: 2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase OS=Glycyrrhiza echinata GN=HI4'OMT PE=2 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 1.259e-9
Identity = 46/108 (42.59%), Postives = 54/108 (50.00%), Query Frame = -1

Query:  142 ADAVLLKWVLHGWNDEQS*RY*RTSKEAXXXXXXXXXXXXXXXXINETSDDRELTGLQLDI*FGDADNVPWKRKRKERMGETYLRCWFQ*LQKLEP*DGFKSIIEVFP 465
            ADAVLLKWVLH WNDE S +  + SKEA                I+E S DRELT LQLD           K + K+   +      F    K+ P  GFKS+IEVFP
Sbjct:  261 ADAVLLKWVLHDWNDELSLKILKNSKEAISGKGKEGKVIIIDISIDEASGDRELTELQLDYDLVMLTMFNGKEREKKEWEKLISDAGFS-SYKITPICGFKSLIEVFP 367

[Close]

BLAST of HO066418 vs. TrEMBL
Match: Q5W2B2_9ROSA (Putative orcinol O-methyltransferase (Fragment) OS=Rosa gallica GN=oomt2B PE=4 SV=1)

HSP 1 Score: 47.3654 bits (111), Expect = 2.083e-9
Identity = 19/32 (59.38%), Postives = 24/32 (75.00%), Query Frame = -2

Query:  189 YDLVMLTMFLGKEREKKEWEKLIYDAGFSSYK 284
            +D++M+ +  GKER KKEW KL  DAGFS YK
Sbjct:  310 FDMLMMALLTGKERNKKEWAKLFTDAGFSDYK 341

HSP 2 Score: 39.2762 bits (90), Expect = 2.083e-9
Identity = 16/28 (57.14%), Postives = 21/28 (75.00%), Query Frame = -1

Query:  382 ADAVLLKWVLHGWNDEQS*RY*RTSKEA 465
            AD VLLKW+LH WNDE+  +  + S+EA
Sbjct:  250 ADTVLLKWILHDWNDEECVKILKRSREA 277

[Close]

BLAST of HO066418 vs. TrEMBL
Match: Q5W2B1_ROSHC (Putative orcinol O-methyltransferase (Fragment) OS=Rosa hybrid cultivar GN=oomt2A PE=4 SV=1)

HSP 1 Score: 47.3654 bits (111), Expect = 2.083e-9
Identity = 19/32 (59.38%), Postives = 24/32 (75.00%), Query Frame = -2

Query:  189 YDLVMLTMFLGKEREKKEWEKLIYDAGFSSYK 284
            +D++M+ +  GKER KKEW KL  DAGFS YK
Sbjct:  310 FDMLMMALLTGKERNKKEWAKLFTDAGFSDYK 341

HSP 2 Score: 39.2762 bits (90), Expect = 2.083e-9
Identity = 16/28 (57.14%), Postives = 21/28 (75.00%), Query Frame = -1

Query:  382 ADAVLLKWVLHGWNDEQS*RY*RTSKEA 465
            AD VLLKW+LH WNDE+  +  + S+EA
Sbjct:  250 ADTVLLKWILHDWNDEECVKILKRSREA 277

[Close]

BLAST of HO066418 vs. TrEMBL
Match: A2AX09_9ROSA (Putative orcinol O-methyltransferase (Fragment) OS=Rosa phoenicia GN=oomtA PE=4 SV=1)

HSP 1 Score: 47.3654 bits (111), Expect = 2.083e-9
Identity = 19/32 (59.38%), Postives = 24/32 (75.00%), Query Frame = -2

Query:  189 YDLVMLTMFLGKEREKKEWEKLIYDAGFSSYK 284
            +D++M+ +  GKER KKEW KL  DAGFS YK
Sbjct:  310 FDMLMMALLTGKERNKKEWAKLFTDAGFSDYK 341

HSP 2 Score: 39.2762 bits (90), Expect = 2.083e-9
Identity = 16/28 (57.14%), Postives = 21/28 (75.00%), Query Frame = -1

Query:  382 ADAVLLKWVLHGWNDEQS*RY*RTSKEA 465
            AD VLLKW+LH WNDE+  +  + S+EA
Sbjct:  250 ADTVLLKWILHDWNDEECVKILKRSREA 277

[Close]

BLAST of HO066418 vs. TrEMBL
Match: Q5W2A1_ROSGI (Putative orcinol O-methyltransferase (Fragment) OS=Rosa gigantea GN=oomt2C PE=4 SV=1)

HSP 1 Score: 45.4394 bits (106), Expect = 2.704e-9
Identity = 18/32 (56.25%), Postives = 24/32 (75.00%), Query Frame = -2

Query:  189 YDLVMLTMFLGKEREKKEWEKLIYDAGFSSYK 284
            +D++M+ +  GKER +KEW KL  DAGFS YK
Sbjct:  310 FDMLMMALVTGKERNEKEWAKLFTDAGFSDYK 341

HSP 2 Score: 40.817 bits (94), Expect = 2.704e-9
Identity = 17/29 (58.62%), Postives = 22/29 (75.86%), Query Frame = -1

Query:  382 LADAVLLKWVLHGWNDEQS*RY*RTSKEA 468
            LAD VLLKW+LH WNDE+  +  + S+EA
Sbjct:  249 LADTVLLKWILHDWNDEECVKILKQSREA 277

[Close]

BLAST of HO066418 vs. TrEMBL
Match: A2AWX4_9ROSA (Putative orcinol O-methyltransferase (Fragment) OS=Rosa bracteata GN=oomtB PE=4 SV=1)

HSP 1 Score: 45.4394 bits (106), Expect = 2.704e-9
Identity = 18/32 (56.25%), Postives = 24/32 (75.00%), Query Frame = -2

Query:  189 YDLVMLTMFLGKEREKKEWEKLIYDAGFSSYK 284
            +D++M+ +  GKER +KEW KL  DAGFS YK
Sbjct:  310 FDMLMMALVTGKERNEKEWAKLFTDAGFSDYK 341

HSP 2 Score: 40.817 bits (94), Expect = 2.704e-9
Identity = 17/28 (60.71%), Postives = 22/28 (78.57%), Query Frame = -1

Query:  382 ADAVLLKWVLHGWNDEQS*RY*RTSKEA 465
            ADAVLLKW+LH WNDE+  +  + S+EA
Sbjct:  250 ADAVLLKWILHDWNDEECVKILKRSREA 277

[Close]

BLAST of HO066418 vs. TrEMBL
Match: A2AWX3_9ROSA (Putative orcinol O-methyltransferase (Fragment) OS=Rosa bracteata GN=oomtA PE=4 SV=1)

HSP 1 Score: 45.4394 bits (106), Expect = 2.704e-9
Identity = 18/32 (56.25%), Postives = 24/32 (75.00%), Query Frame = -2

Query:  189 YDLVMLTMFLGKEREKKEWEKLIYDAGFSSYK 284
            +D++M+ +  GKER +KEW KL  DAGFS YK
Sbjct:  310 FDMLMMALVTGKERNEKEWAKLFTDAGFSDYK 341

HSP 2 Score: 40.817 bits (94), Expect = 2.704e-9
Identity = 17/28 (60.71%), Postives = 22/28 (78.57%), Query Frame = -1

Query:  382 ADAVLLKWVLHGWNDEQS*RY*RTSKEA 465
            ADAVLLKW+LH WNDE+  +  + S+EA
Sbjct:  250 ADAVLLKWILHDWNDEECVKILKRSREA 277

[Close]

BLAST of HO066418 vs. TrEMBL
Match: A2AWW8_9ROSA (Putative orcinol O-methyltransferase (Fragment) OS=Rosa banksiae GN=oomtB PE=4 SV=1)

HSP 1 Score: 45.4394 bits (106), Expect = 2.704e-9
Identity = 18/32 (56.25%), Postives = 24/32 (75.00%), Query Frame = -2

Query:  189 YDLVMLTMFLGKEREKKEWEKLIYDAGFSSYK 284
            +D++M+ +  GKER +KEW KL  DAGFS YK
Sbjct:  310 FDMLMMALVTGKERNEKEWAKLFTDAGFSDYK 341

HSP 2 Score: 40.817 bits (94), Expect = 2.704e-9
Identity = 17/28 (60.71%), Postives = 22/28 (78.57%), Query Frame = -1

Query:  382 ADAVLLKWVLHGWNDEQS*RY*RTSKEA 465
            ADAVLLKW+LH WNDE+  +  + S+EA
Sbjct:  250 ADAVLLKWILHDWNDEECVKILKRSREA 277

The following BLAST results are available for this feature:

BLAST of HO066418 vs. SwissProt
BLAST of HO066418 vs. TrEMBL

BLAST of HO066418 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
EOMT1_OCIBA	2.637e-7	62.50	Eugenol O-methyltransferase OS=Ocimum basilicum GN...	[more]
CVMT1_OCIBA	5.734e-7	56.25	Chavicol O-methyltransferase OS=Ocimum basilicum G...	[more]

BLAST of HO066418 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q29U70_MEDTR	1.345e-11	40.97	SAM dependent isoflavanone 4'-O-methyltransferase/...	[more]
Q84KK4_LOTJA	1.942e-10	39.58	S-adenosyl-L-methionine: 2,7,4'-trihydroxyisoflava...	[more]
Q84KK6_GLYEC	1.259e-9	42.59	S-adenosyl-L-methionine: 2,7,4'-trihydroxyisoflava...	[more]
Q5W2B2_9ROSA	2.083e-9	59.38	Putative orcinol O-methyltransferase (Fragment) OS...	[more]
Q5W2B1_ROSHC	2.083e-9	59.38	Putative orcinol O-methyltransferase (Fragment) OS...	[more]
A2AX09_9ROSA	2.083e-9	59.38	Putative orcinol O-methyltransferase (Fragment) OS...	[more]
Q5W2A1_ROSGI	2.704e-9	56.25	Putative orcinol O-methyltransferase (Fragment) OS...	[more]
A2AWX4_9ROSA	2.704e-9	56.25	Putative orcinol O-methyltransferase (Fragment) OS...	[more]
A2AWX3_9ROSA	2.704e-9	56.25	Putative orcinol O-methyltransferase (Fragment) OS...	[more]
A2AWW8_9ROSA	2.704e-9	56.25	Putative orcinol O-methyltransferase (Fragment) OS...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 19..29 score
None	No IPR available	SEG	seg	seg	coord: 10..19 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO066418 ID=HO066418; Name=HO066418; organism=Cicer arietinum; type=EST; length=699bp
GTTTGGGAAATTCGTTTCTTATAGTGAATCAATAAACACCAATACAATCC
AAAAAATAAAGACGCTATTAATTTGACTCTCAAATAGAAACTTATTAAAT
ATGAGGCATAACTGGACCCAAGCTGCTTCAAGTTAAAGTTAAGGAAAAAC
TTCAATGATGGACTTAAAGCCATCTTATGGTTCTAATTTTTGTAACTACT
GAAACCAGCATCGTAAATAAGTTTCTCCCATTCTTTCTTTTCTCTTTCTT
TTCCAAGGAACATTGTCAGCATCACCAAATCATATGTCCAATTGCAACCC
AGTTAATTCACGATCATCACTTGTTTCATTGATTGATATGTCGATGATAA
TCACTTTTCCATCTTTCCCTTTGCCAGAAATAGCTTCTTTACTTGTTCTT
CAATATCTTCAAGATTGTTCATCATTCCAACCGTGTAAAACCCACTTGAG
TAAAACAGCATCTGCAAGGAGGAATAGACTTGAAACATATCTCCAGTCAA
CAAAGTTTCAAATTTTCAGTNCCAGTNAAGTTACACAACAACACTGAGGT
TGATAAAAAACAGTGCCTTTAAGTGTGAGGCAAAGNTTTATTGACNGACG
TTTTAGATGNACACCAAAGTTCTCTTCAANTATAAGAAGAATCACAACCT
CAAAAACTTGTTGTTCTNTGANAAGCAGTCAAATTAAAATAAAGACAAG

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