HO664296

EST Overview


Name	HO664296
Unique Name	HO664296
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	528

Libraries


Library Name	Type
LIBEST_026619 TDZ treated Chickpea SSH library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of HO664296 vs. SwissProt
Match: ALG8_ARATH (Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Arabidopsis thaliana GN=At2g44660 PE=2 SV=2)

HSP 1 Score: 234.572 bits (597), Expect = 2.297e-61
Identity = 111/162 (68.52%), Postives = 127/162 (78.40%), Query Frame = 1

Query:   19 YFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRLTQKLDLRMQKLIWPLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLGGFVFAVLLCFKHLFAVAAPVYFIYLLRHYCWGGIVPG 504
            YFDETS WTLDYPPFFAYFERFLSI A  VDP+IV LQ GL+Y++  V++FQR ++I+SDL LLYGVYRLT+KL+   + LI  LVIWSP LLIVDH+HFQYNGF               RDLLGGF+FAVLLCFKHLFAV APVYF+YLLRHYCW G+V G
Sbjct:   54 YFDETSQWTLDYPPFFAYFERFLSIFARLVDPRIVDLQSGLDYNAESVIYFQRISVIVSDLCLLYGVYRLTRKLEPLKRNLICALVIWSPGLLIVDHIHFQYNGFLLGWLLLSISFLQEGRDLLGGFLFAVLLCFKHLFAVTAPVYFVYLLRHYCWSGLVTG 215

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BLAST of HO664296 vs. SwissProt
Match: ALG8_EMENI (Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Emericella nidulans GN=alg8 PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 2.926e-32
Identity = 76/159 (47.80%), Postives = 95/159 (59.75%), Query Frame = 1

Query:   19 YFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRLTQKLDLRMQKLIWPL---VIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLL-GGFVFAVLLCFKHLFAVAAPVYFIYLLRHYC 483
            Y+++TS WTLDYPPFFA FE  LS LA  VDP ++ +Q  LNY S + V+FQRAT+ILS+L L Y + R  +      + L       ++ SP L I+DH+HFQYNGF                 LL  G  FAVLLC KH++   AP YFIYLLR YC
Sbjct:   50 YYEKTSEWTLDYPPFFAVFEWALSQLAQYVDPAMLDVQ-NLNYDSWQTVYFQRATVILSELVLFYALNRFIRSDPQPTKHLAHAASLSILLSPGLFIIDHIHFQYNGFLYGILVLSIVWARKQSKLLYSGIAFAVLLCLKHIYLYLAPAYFIYLLRAYC 207

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BLAST of HO664296 vs. SwissProt
Match: ALG8_GIBZE (Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Gibberella zeae GN=ALG8 PE=3 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 2.477e-31
Identity = 69/159 (43.40%), Postives = 99/159 (62.26%), Query Frame = 1

Query:   19 YFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRLTQKLDLRMQKLIWPL---VIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLG-GFVFAVLLCFKHLFAVAAPVYFIYLLRHYC 483
            Y ++TS WTLDYPPFFAYFE  L+ +A  VDP +V +   L+Y S + V+FQR ++I+++L L++ +    +   L+ ++    +   +I SP LLI+DH+HFQYNGF                +LL  G +FA LLCFKH++   AP YF+YLLR YC
Sbjct:   50 YVEKTSEWTLDYPPFFAYFEWILAHVARLVDPLMVKVYN-LDYDSWQTVYFQRTSVIVTELVLVWALQTFIETAPLKSRRAAQTVALSIILSPGLLIIDHIHFQYNGFMYGILVMSLVLARQNSELLSSGLIFAALLCFKHIYLYLAPAYFVYLLRAYC 207

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BLAST of HO664296 vs. SwissProt
Match: ALG8_BOVIN (Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Bos taurus GN=ALG8 PE=2 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.097e-30
Identity = 67/165 (40.61%), Postives = 101/165 (61.21%), Query Frame = 1

Query:   19 YFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRLTQKLDLRM---------QKLIWPLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLGGFVFAVLLCFKHLFAVAAPVYFIYLLRHYCW 486
            Y++ TS WTLDYPPFFA+FE  LS +A   D ++++++  LNYSS++ + FQR ++I +D   +Y V+   + +D +          + ++  L++W+  LLIVDH+HFQYNGF               R + G F+FAVLL FKH++   AP Y +YLLR YC+
Sbjct:   56 YYEATSEWTLDYPPFFAWFEYALSHVAKYFDQEMLNVRN-LNYSSSRTLLFQRFSVIFTDALFVYAVHECCKCIDGKKAGKELTEKPKFILSALLLWNFGLLIVDHIHFQYNGFLSGLMLLSIARFFQKRHMEGAFLFAVLLHFKHIYLYVAPAYGVYLLRSYCF 219

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BLAST of HO664296 vs. SwissProt
Match: ALG8_HUMAN (Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Homo sapiens GN=ALG8 PE=1 SV=2)

HSP 1 Score: 130.954 bits (328), Expect = 3.577e-30
Identity = 67/165 (40.61%), Postives = 101/165 (61.21%), Query Frame = 1

Query:   19 YFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVY---------RLTQKLDLRMQKLIWPLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLGGFVFAVLLCFKHLFAVAAPVYFIYLLRHYCW 486
            Y++ TS WTLDYPPFFA+FE  LS +A   D +++++   LNYSS++ + FQR ++I  D+  +Y V          ++ ++L  + + ++  L++W+  LLIVDH+HFQYNGF               R + G F+FAVLL FKH++   AP Y +YLLR YC+
Sbjct:   56 YYEATSEWTLDYPPFFAWFEYILSHVAKYFDQEMLNVHN-LNYSSSRTLLFQRFSVIFMDVLFVYAVRECCKCIDGKKVGKELTEKPKFILSVLLLWNFGLLIVDHIHFQYNGFLFGLMLLSIARLFQKRHMEGAFLFAVLLHFKHIYLYVAPAYGVYLLRSYCF 219

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BLAST of HO664296 vs. SwissProt
Match: ALG8_NEUCR (Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Neurospora crassa GN=alg-8 PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 6.101e-30
Identity = 70/158 (44.30%), Postives = 93/158 (58.86%), Query Frame = 1

Query:   19 YFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYG--VYRLTQKLDLRMQKLIWPLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLL-GGFVFAVLLCFKHLFAVAAPVYFIYLLRHYC 483
            Y+++TS WTLDYPPFFAYFE  +S +A   DP ++ +   L Y S + V+FQR T+I+++L LLY   +Y+      LR        ++ SP LLI+DH+HFQYNG                  LL  G VFA LLC KH++   AP YF+YLLR YC
Sbjct:   50 YYEKTSEWTLDYPPFFAYFEWIMSQVARLADPAMIWV-HNLEYDSWQTVYFQRWTVIVTELVLLYALQMYKGILSGSLRAAHAAAVSILLSPGLLIIDHIHFQYNGVMYGILIASLVLAKKKSSLLASGLVFAALLCMKHIYLYLAPAYFVYLLRVYC 206

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BLAST of HO664296 vs. SwissProt
Match: ALG8_MOUSE (Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Mus musculus GN=Alg8 PE=2 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 5.165e-29
Identity = 67/170 (39.41%), Postives = 99/170 (58.24%), Query Frame = 1

Query:   19 YFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVY---------RLTQKLDLRMQKLIWPLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLGGFVFAVLLCFKHLFAVAAPVYFIYLLRHYCWGGIVP 501
            Y++ TS WTLDYPPFFA+FE  LS +A   D +++++   LNY S++ + FQR ++IL+D   +Y V+         R  + L  + + ++  L++W+  LLIVDH+HFQYNGF               R + G  +FAVLL  KH++   AP Y +YLLR YC+    P
Sbjct:   56 YYEATSEWTLDYPPFFAWFEYALSHIAKYFDQEMLNIHN-LNYYSSRTLLFQRFSVILTDALFVYAVHECCKCIDGKRTGKDLTEKPKFILSVLLLWNFGLLIVDHIHFQYNGFLSGLLLLSIARLFQKRHIEGALLFAVLLHLKHIYLYVAPAYGVYLLRSYCFTASKP 224

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BLAST of HO664296 vs. SwissProt
Match: ALG8_ASPOR (Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Aspergillus oryzae GN=alg8 PE=3 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 1.151e-28
Identity = 66/159 (41.51%), Postives = 96/159 (60.38%), Query Frame = 1

Query:   19 YFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRLTQ---KLDLRMQKLIWPLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLL-GGFVFAVLLCFKHLFAVAAPVYFIYLLRHYC 483
            Y+++TS WTLDYPPFFA FE  LS +A   DP ++ + + LNY S + ++FQRAT+I+S+L L++ + R  +   + +  +  +    ++ SP LLI+DH+HFQYNGF                 LL  G  FA+LLC KH+    +  YF+YLLR YC
Sbjct:   50 YYEKTSEWTLDYPPFFAAFEWLLSQVAQYADPAMLTV-KNLNYDSWQTIYFQRATVIISELVLVFALNRFIKSAPQSNKHLAHISSLSILLSPGLLIIDHIHFQYNGFMYGVLILSIVLARKQSTLLYSGITFAILLCMKHIHLYLSLAYFVYLLRTYC 207

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BLAST of HO664296 vs. SwissProt
Match: ALG8_COCIM (Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Coccidioides immitis GN=ALG8 PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 1.963e-28
Identity = 66/159 (41.51%), Postives = 94/159 (59.12%), Query Frame = 1

Query:   19 YFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRLTQKLDLRMQKLIWPL---VIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLL-GGFVFAVLLCFKHLFAVAAPVYFIYLLRHYC 483
            Y+++TS WTLDYPPFFA  E  LS +A+ VDP ++ L   LNY S + ++FQR+++I  +L L+Y + R  + +    + L       ++ SP LLI+DH+HFQYNGF                 LL  G  FA+LLC KH++   +  YF+YLLR YC
Sbjct:   50 YYEKTSEWTLDYPPFFAGLEWLLSKVAFFVDPAMLQL-GNLNYDSWQTIYFQRSSVIFLELMLVYALNRYIKSVPAPSKHLAHAASLSILLSPGLLIIDHIHFQYNGFLYGILILSIVLARKQSTLLYSGVTFAILLCLKHIYLYLSLAYFVYLLRAYC 207

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BLAST of HO664296 vs. SwissProt
Match: ALG8_DROME (Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Drosophila melanogaster GN=CG4542 PE=2 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 4.372e-28
Identity = 69/159 (43.40%), Postives = 90/159 (56.60%), Query Frame = 1

Query:   19 YFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRLTQKLDLRMQKLIW----PLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLGGFVFAVLLCFKHLFAVAAPVYFIYLLRHYC 483
            Y D TS WTLDYPPFFAYFE  LS +A  VDP+++ + + LNY S   V+FQR ++I++DL  + GV      L L      +     L++ +  L+ VDH+HFQYNG                R L   F FAVLL FKH+F   AP + +YLLR YC
Sbjct:   47 YVDATSEWTLDYPPFFAYFEWLLSQVAKYVDPRML-VVDNLNYESKATVYFQRLSVIVTDLVYVLGVRSCLGSLGLGRDTQQFFAASMLLLLNVGLIFVDHIHFQYNGLLFGILLLSIGSLIRQRFLWSAFAFAVLLNFKHIFLYMAPAFGVYLLRFYC 204

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BLAST of HO664296 vs. TrEMBL
Match: D7LLP2_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_903892 PE=4 SV=1)

HSP 1 Score: 234.187 bits (596), Expect = 3.830e-60
Identity = 111/162 (68.52%), Postives = 127/162 (78.40%), Query Frame = 1

Query:   19 YFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRLTQKLDLRMQKLIWPLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLGGFVFAVLLCFKHLFAVAAPVYFIYLLRHYCWGGIVPG 504
            YFDETS WTLDYPPFFAYFERFLSI A  +DP+IV LQ GL+YSS  V++FQR ++I+SDL LL GVYRLT+KL+   + LI  +VIWSP LLIVDH+HFQYNGF               RDLLGGF+FAVLLCFKHLFAVAAPVYF+YLLRHYCW G+V G
Sbjct:   54 YFDETSQWTLDYPPFFAYFERFLSIFARLIDPRIVDLQSGLDYSSESVIYFQRISVIVSDLCLLCGVYRLTRKLEPMKRNLICAMVIWSPGLLIVDHIHFQYNGFLLGWLLLSISFLQEGRDLLGGFLFAVLLCFKHLFAVAAPVYFVYLLRHYCWSGLVTG 215

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BLAST of HO664296 vs. TrEMBL
Match: A9RXF0_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_179078 PE=4 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 3.848e-44
Identity = 84/157 (53.50%), Postives = 108/157 (68.79%), Query Frame = 1

Query:   19 YFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRLTQKLDLRMQKLIWPLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLGGFVFAVLLCFKHLFAVAAPVYFIYLLRHYCWG 489
            Y DE+S WTLDYPPFFA+FERFL+I A   DPQIV L  G NY+   VV FQR T++ +DL L +G++ +   L    +++++ +VI+SP LLIVDH+HFQYNGF                DLLGG  FA L+CFKHLFA+A P+YF+Y+LRHYC G
Sbjct:   51 YSDESSQWTLDYPPFFAFFERFLAIFASWFDPQIVDLVNGQNYAVRSVVLFQRGTVMAADLVLYWGLWEIGSGLSRMRRRILYLVVIFSPGLLIVDHIHFQYNGFLFGILFLSLAAMRDGNDLLGGIYFAALVCFKHLFAIAGPIYFVYILRHYCKG 207

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BLAST of HO664296 vs. TrEMBL
Match: B9S6A4_RICCO (Dolichyl glycosyltransferase, putative OS=Ricinus communis GN=RCOM_0534290 PE=4 SV=1)

HSP 1 Score: 174.866 bits (442), Expect = 2.758e-42
Identity = 84/162 (51.85%), Postives = 104/162 (64.20%), Query Frame = 1

Query:   19 YFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRLTQKLDLRMQKLIWPLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLGGFVFAVLLCFKHLFAVAAPVYFIYLLRHYCWGGIVPG 504
            YFDETSPWTLDYPPFFAY+E  LS+ A  +DPQ+V +  GLNY SN V++FQR ++I+SDL LLYG+YRLT+ L+ R + L+W LV+WSP L++VDH+HFQYN                                     VAAPVYF+YLLRHYCW GI  G
Sbjct:   60 YFDETSPWTLDYPPFFAYYEYILSLFAQFIDPQMVDIYRGLNYKSNTVIYFQRISVIISDLCLLYGIYRLTKNLESRKRILMWVLVVWSPGLIMVDHMHFQYN-------------------------------------VAAPVYFVYLLRHYCWKGISMG 184

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BLAST of HO664296 vs. TrEMBL
Match: Q6ZGZ7_ORYSJ (Putative glucosyltransferase OS=Oryza sativa subsp. japonica GN=OJ1743_B12.13 PE=4 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 7.274e-35
Identity = 88/174 (50.57%), Postives = 101/174 (58.05%), Query Frame = 1

Query:    7 SRPRYFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRL--TQKLDLRMQK--LIWPLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLGGFVFAVLLCFKHLFAVAAPVYFIYLLRHYCWG-GIVPGPGR 513
            +R  Y D +S WTLDYPPFFAYF R L++ A  VD  +V L       S   + + R T+  SDL LL     L  T  L  R ++  L   LV+WSP LL VDHVHFQYNGF               RDL GG VFA LLC KHLF VAAPVYF+YL RHYC G G+V G GR
Sbjct:   51 ARRWYTDASSQWTLDYPPFFAYFSRLLALPAPLVDASLVSLPVPDAPPSFAYLLYLRLTVAFSDLLLLLSSVLLLATDALTRRRRRPFLSLALVLWSPALLAVDHVHFQYNGFLMGLLLLSLHFLEQGRDLAGGVVFAALLCSKHLFLVAAPVYFVYLFRHYCCGRGVVRGLGR 224

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BLAST of HO664296 vs. TrEMBL
Match: Q0DYJ5_ORYSJ (Os02g0688500 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os02g0688500 PE=4 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 7.274e-35
Identity = 88/174 (50.57%), Postives = 101/174 (58.05%), Query Frame = 1

Query:    7 SRPRYFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRL--TQKLDLRMQK--LIWPLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLGGFVFAVLLCFKHLFAVAAPVYFIYLLRHYCWG-GIVPGPGR 513
            +R  Y D +S WTLDYPPFFAYF R L++ A  VD  +V L       S   + + R T+  SDL LL     L  T  L  R ++  L   LV+WSP LL VDHVHFQYNGF               RDL GG VFA LLC KHLF VAAPVYF+YL RHYC G G+V G GR
Sbjct:   48 ARRWYTDASSQWTLDYPPFFAYFSRLLALPAPLVDASLVSLPVPDAPPSFAYLLYLRLTVAFSDLLLLLSSVLLLATDALTRRRRRPFLSLALVLWSPALLAVDHVHFQYNGFLMGLLLLSLHFLEQGRDLAGGVVFAALLCSKHLFLVAAPVYFVYLFRHYCCGRGVVRGLGR 221

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BLAST of HO664296 vs. TrEMBL
Match: A2X8F8_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_08520 PE=4 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 7.274e-35
Identity = 88/174 (50.57%), Postives = 101/174 (58.05%), Query Frame = 1

Query:    7 SRPRYFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRL--TQKLDLRMQK--LIWPLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLGGFVFAVLLCFKHLFAVAAPVYFIYLLRHYCWG-GIVPGPGR 513
            +R  Y D +S WTLDYPPFFAYF R L++ A  VD  +V L       S   + + R T+  SDL LL     L  T  L  R ++  L   LV+WSP LL VDHVHFQYNGF               RDL GG VFA LLC KHLF VAAPVYF+YL RHYC G G+V G GR
Sbjct:   51 ARRWYTDASSQWTLDYPPFFAYFSRLLALPAPLVDASLVSLPVPDAPPSFAYLLYLRLTVAFSDLLLLLSSVLLLATDALTRRRRRPFLSLALVLWSPALLAVDHVHFQYNGFLMGLLLLSLHFLEQGRDLAGGVVFAALLCSKHLFLVAAPVYFVYLFRHYCCGRGVVRGLGR 224

[Close]

BLAST of HO664296 vs. TrEMBL
Match: D7SM34_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_21.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00018341001 PE=4 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 9.500e-35
Identity = 83/167 (49.70%), Postives = 90/167 (53.89%), Query Frame = 1

Query:   19 YFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRLTQKLDLRMQKLIWPLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLGGFVFAVLLCFKHLFAVAAPVYFIYLLRHYCWGGIVPGPGRPL 519
            Y DETSPWTLDYPPFFAYFERFLSI A  +DP I                                            + LIW LV  SP L IVDHVHFQYNGF               RDL+GGFVFAVLLCFKHLFAVAAPVYF++LL  +C GG + G GR L
Sbjct:   61 YSDETSPWTLDYPPFFAYFERFLSIFANLIDPTI--------------------------------------------RNLIWVLVASSPGLFIVDHVHFQYNGFLLGILLLSLSFLEEGRDLMGGFVFAVLLCFKHLFAVAAPVYFVFLLSRFCRGGFLKGFGRVL 183

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BLAST of HO664296 vs. TrEMBL
Match: B0CY11_LACBS (Glucosyltransferase OS=Laccaria bicolor (strain S238N-H82) GN=ALG8 PE=4 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 8.043e-34
Identity = 79/156 (50.64%), Postives = 98/156 (62.82%), Query Frame = 1

Query:   19 YFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRLTQ-KLDLRMQKLIWPLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLGGFVFAVLLCFKHLFAVAAPVYFIYLLRHYC 483
            YFD TS WTLDYPPFFAYFE+FLSI A  VDP+IV L   LNY S  VV +QRAT+IL++L L   V    +  ++  +Q++I   +   P  LIVDH+HFQYNGF                 L+ G +FAVLL FKH++   AP YFIYLLR +C
Sbjct:   74 YFDTTSEWTLDYPPFFAYFEKFLSIFALLVDPKIVDL-NNLNYDSWTVVAYQRATVILTELVLGTAVLGFIRGSVEPPVQRIISAALFLHPGFLIVDHIHFQYNGFMFGILLWSILMARNGNKLVSGILFAVLLNFKHIYIYLAPAYFIYLLRSFC 228

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BLAST of HO664296 vs. TrEMBL
Match: C5Y0U4_SORBI (Putative uncharacterized protein Sb04g031440 OS=Sorghum bicolor GN=Sb04g031440 PE=4 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 8.892e-33
Identity = 84/171 (49.12%), Postives = 99/171 (57.89%), Query Frame = 1

Query:    7 SRPRYFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRLTQKLDLRMQK-LIWPLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLGGFVFAVLLCFKHLFAVAAPVYFIYLLRHYCWG-GIVPGPGR 513
            +R  Y D +S WTLDYPPFFAYF R LS+ A  VD  +V +    +      + + R T+  SDL LL  V  L +    + +  L   LV+WSP LL VDHVHFQYNGF                DL GG VFA LLC KHLF VAAPVYF+YL RHYC G G+V G GR
Sbjct:   53 ARQWYTDASSQWTLDYPPFFAYFSRILSLPAPLVDAALVSVPVP-DAPPFAHLLYLRLTVAFSDLLLLGSVLLLARDARRKQRPFLALALVLWSPALLAVDHVHFQYNGFLMGLLLLSLHFLEQGWDLAGGVVFASLLCSKHLFLVAAPVYFMYLFRHYCCGRGVVKGLGR 222

[Close]

BLAST of HO664296 vs. TrEMBL
Match: C8VCQ5_EMENI (Putative uncharacterized protein OS=Aspergillus nidulans FGSC A4 GN=ANIA_07301 PE=4 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 3.736e-31
Identity = 76/159 (47.80%), Postives = 95/159 (59.75%), Query Frame = 1

Query:   19 YFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRLTQKLDLRMQKLIWPL---VIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLL-GGFVFAVLLCFKHLFAVAAPVYFIYLLRHYC 483
            Y+++TS WTLDYPPFFA FE  LS LA  VDP ++ +Q  LNY S + V+FQRAT+ILS+L L Y + R  +      + L       ++ SP L I+DH+HFQYNGF                 LL  G  FAVLLC KH++   AP YFIYLLR YC
Sbjct:   50 YYEKTSEWTLDYPPFFAVFEWALSQLAQYVDPAMLDVQ-NLNYDSWQTVYFQRATVILSELVLFYALNRFIRSDPQPTKHLAHAASLSILLSPGLFIIDHIHFQYNGFLYGILVLSIVWARKQSKLLYSGIAFAVLLCLKHIYLYLAPAYFIYLLRAYC 207

[Close]

BLAST of HO664296 vs. TAIR peptide
Match: AT2G44660.1 (| Symbols: | ALG6, ALG8 glycosyltransferase family | chr2:18420832-18422827 REVERSE LENGTH=506)

HSP 1 Score: 234.572 bits (597), Expect = 2.476e-62
Identity = 111/162 (68.52%), Postives = 127/162 (78.40%), Query Frame = 1

Query:   19 YFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYGVYRLTQKLDLRMQKLIWPLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLGGFVFAVLLCFKHLFAVAAPVYFIYLLRHYCWGGIVPG 504
            YFDETS WTLDYPPFFAYFERFLSI A  VDP+IV LQ GL+Y++  V++FQR ++I+SDL LLYGVYRLT+KL+   + LI  LVIWSP LLIVDH+HFQYNGF               RDLLGGF+FAVLLCFKHLFAV APVYF+YLLRHYCW G+V G
Sbjct:   54 YFDETSQWTLDYPPFFAYFERFLSIFARLVDPRIVDLQSGLDYNAESVIYFQRISVIVSDLCLLYGVYRLTRKLEPLKRNLICALVIWSPGLLIVDHIHFQYNGFLLGWLLLSISFLQEGRDLLGGFLFAVLLCFKHLFAVTAPVYFVYLLRHYCWSGLVTG 215

[Close]

BLAST of HO664296 vs. TAIR peptide
Match: AT5G38460.2 (| Symbols: | ALG6, ALG8 glycosyltransferase family | chr5:15398592-15400987 REVERSE LENGTH=533)

HSP 1 Score: 58.5362 bits (140), Expect = 2.432e-9
Identity = 41/165 (24.85%), Postives = 71/165 (43.03%), Query Frame = 1

Query:    4 WSRPRYFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYG-------VYRLTQKLDLRMQKLIW--PLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLGGFVFAVLLCFKHLFAVAAPVYFIYLL 471
            W R   +++ + W LDYPP  AY      I     +P+ V L     + S       R T++ SD  + +        VY   +    +  ++ W   +++ +P L+++DH HFQYN                  ++L   +F++ L  K + A  AP +F +LL
Sbjct:   91 WYRNGTYNDLTYWGLDYPPLTAYQSYIHGIFLRFFNPESVALLSSRGHESYLGKLLMRWTVLSSDAFIFFPAALFFVLVYHRNRTRGGK-SEVAWHIAMILLNPCLILIDHGHFQYNCISLGLTVGAIAAVLCESEVLTCVLFSLALSHKQMSAYFAPAFFSHLL 254

[Close]

BLAST of HO664296 vs. TAIR peptide
Match: AT5G38460.1 (| Symbols: | ALG6, ALG8 glycosyltransferase family | chr5:15398592-15400987 REVERSE LENGTH=533)

HSP 1 Score: 58.5362 bits (140), Expect = 2.432e-9
Identity = 41/165 (24.85%), Postives = 71/165 (43.03%), Query Frame = 1

Query:    4 WSRPRYFDETSPWTLDYPPFFAYFERFLSILAYQVDPQIVHLQEGLNYSSNKVVFFQRATIILSDLSLLYG-------VYRLTQKLDLRMQKLIW--PLVIWSPMLLIVDHVHFQYNGFXXXXXXXXXXXXXXXRDLLGGFVFAVLLCFKHLFAVAAPVYFIYLL 471
            W R   +++ + W LDYPP  AY      I     +P+ V L     + S       R T++ SD  + +        VY   +    +  ++ W   +++ +P L+++DH HFQYN                  ++L   +F++ L  K + A  AP +F +LL
Sbjct:   91 WYRNGTYNDLTYWGLDYPPLTAYQSYIHGIFLRFFNPESVALLSSRGHESYLGKLLMRWTVLSSDAFIFFPAALFFVLVYHRNRTRGGK-SEVAWHIAMILLNPCLILIDHGHFQYNCISLGLTVGAIAAVLCESEVLTCVLFSLALSHKQMSAYFAPAFFSHLL 254

The following BLAST results are available for this feature:

BLAST of HO664296 vs. SwissProt
BLAST of HO664296 vs. TrEMBL
BLAST of HO664296 vs. TAIR peptide

BLAST of HO664296 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ALG8_ARATH	2.297e-61	68.52	Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 al...	[more]
ALG8_EMENI	2.926e-32	47.80	Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-g...	[more]
ALG8_GIBZE	2.477e-31	43.40	Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-g...	[more]
ALG8_BOVIN	2.097e-30	40.61	Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 al...	[more]
ALG8_HUMAN	3.577e-30	40.61	Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 al...	[more]
ALG8_NEUCR	6.101e-30	44.30	Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-g...	[more]
ALG8_MOUSE	5.165e-29	39.41	Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 al...	[more]
ALG8_ASPOR	1.151e-28	41.51	Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-g...	[more]
ALG8_COCIM	1.963e-28	41.51	Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-g...	[more]
ALG8_DROME	4.372e-28	43.40	Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 al...	[more]

BLAST of HO664296 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7LLP2_ARALY	3.830e-60	68.52	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
A9RXF0_PHYPA	3.848e-44	53.50	Predicted protein OS=Physcomitrella patens subsp. ...	[more]
B9S6A4_RICCO	2.758e-42	51.85	Dolichyl glycosyltransferase, putative OS=Ricinus ...	[more]
Q6ZGZ7_ORYSJ	7.274e-35	50.57	Putative glucosyltransferase OS=Oryza sativa subsp...	[more]
Q0DYJ5_ORYSJ	7.274e-35	50.57	Os02g0688500 protein (Fragment) OS=Oryza sativa su...	[more]
A2X8F8_ORYSI	7.274e-35	50.57	Putative uncharacterized protein OS=Oryza sativa s...	[more]
D7SM34_VITVI	9.500e-35	49.70	Whole genome shotgun sequence of line PN40024, sca...	[more]
B0CY11_LACBS	8.043e-34	50.64	Glucosyltransferase OS=Laccaria bicolor (strain S2...	[more]
C5Y0U4_SORBI	8.892e-33	49.12	Putative uncharacterized protein Sb04g031440 OS=So...	[more]
C8VCQ5_EMENI	3.736e-31	47.80	Putative uncharacterized protein OS=Aspergillus ni...	[more]

BLAST of HO664296 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT2G44660.1	2.476e-62	68.52	\| Symbols: \| ALG6, ALG8 glycosyltransferase famil...	[more]
AT5G38460.2	2.432e-9	24.85	\| Symbols: \| ALG6, ALG8 glycosyltransferase famil...	[more]
AT5G38460.1	2.432e-9	24.85	\| Symbols: \| ALG6, ALG8 glycosyltransferase famil...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004856	Glycosyl transferase, ALG6/ALG8	PANTHER	PTHR12413	DOLICHYL GLYCOSYLTRANSFERASE	coord: 2..161 score: 7.5
IPR004856	Glycosyl transferase, ALG6/ALG8	PFAM	PF03155	Alg6_Alg8	coord: 7..161 score: 5.7
None	No IPR available	SEG	seg	seg	coord: 112..126 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO664296 ID=HO664296; Name=HO664296; organism=Cicer arietinum; type=EST; length=528bp
GCGTGGTCGCGGCCGAGGTACTTTGATGAGACCAGTCCATGGACTCTAGA
TTACCCACCATTCTTTGCCTATTTTGAGCGCTTTCTATCAATTTTAGCTT
ACCAAGTTGATCCTCAAATAGTGCATCTTCAGGAGGGCCTGAATTATAGC
TCAAATAAGGTGGTTTTTTTTCAAAGAGCTACCATAATACTCTCTGATTT
GTCCCTTCTTTATGGAGTTTATAGACTTACTCAGAAACTGGATTTGAGAA
TGCAAAAGTTGATATGGCCATTGGTTATTTGGTCACCGATGCTTTTAATA
GTGGACCATGTGCATTTCCAATACAATGGATTTCTCATTGGCATTTTGCT
GATTTCACTTTCACATTTGGAGGAAGGAAGGGATTTGTTAGGTGGCTTTG
TCTTTGCTGTTCTTCTGTGCTTTAAACATTTATTTGCAGTGGCAGCGCCT
GTTTATTTTATTTATTTGTTGAGGCACTATTGTTGGGGTGGAATTGTACC
TGGCCCCGGGCGGCCGCTCGAAATCACT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005789	endoplasmic reticulum membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR004856	Glyco_trans_ALG6/ALG8

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