HO664308

EST Overview


Name	HO664308
Unique Name	HO664308
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	559

Libraries


Library Name	Type
LIBEST_026619 TDZ treated Chickpea SSH library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO664308 vs. SwissProt
Match: DPOE2_CHICK (DNA polymerase epsilon subunit 2 OS=Gallus gallus GN=POLE2 PE=2 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 6.234e-31
Identity = 65/174 (37.36%), Postives = 107/174 (61.49%), Query Frame = 2

Query:   11 GRGTFTKEETIRLAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPPS 532
            G  + + + + +L ++E    + MFV LS++WLD+ E L KL  +  G+ S    P+ F F GNF S P     +   SL+     L  II   P + ++SRF+F+PGP+D GP + LP   L + +T+E ++ +P ++F++NPCRV++ TQE++ FR+DL+ +M R+C+  PS
Sbjct:  263 GPSSASVKASAKLKQLEDENEDAMFVFLSDVWLDQAEVLEKLHTMFSGYSSAP--PTCFFFCGNFSSAP--YGKNQIQSLKGSLKALADIICEYPSIHKSSRFVFVPGPEDPGPGSVLPRPPLAENITQEFRQLVPFSVFTTNPCRVQYCTQEIIIFREDLVNKMCRNCVRFPS 432

[Close]

BLAST of HO664308 vs. SwissProt
Match: DPOE2_MOUSE (DNA polymerase epsilon subunit 2 OS=Mus musculus GN=Pole2 PE=2 SV=2)

HSP 1 Score: 123.25 bits (308), Expect = 8.425e-28
Identity = 57/163 (34.97%), Postives = 101/163 (61.96%), Query Frame = 2

Query:   44 RLAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPPS 532
            +L ++E    + MFVI+S++WLD+ + L K  ++  G+      P+ F+  GNF S P     +   +L+     L  II   P + ++SRF+F+PGP+D G  + LP   L + +T+E ++ +P ++F++NPCR+++ TQE++ FR+DL+ +M R+C+  PS
Sbjct:  274 KLRQLEDENKDAMFVIVSDVWLDQVQVLEKFHVMFSGYSPAP--PTCFILCGNFSSAP--YGKNQVQALKDSLKSLADIICEYPSIHQSSRFVFVPGPEDPGFGSILPRPPLAESITQEFRQRVPFSVFTTNPCRIQYCTQEIIIFREDLVNKMCRNCVRFPS 432

[Close]

BLAST of HO664308 vs. SwissProt
Match: DPOE2_HUMAN (DNA polymerase epsilon subunit 2 OS=Homo sapiens GN=POLE2 PE=1 SV=2)

HSP 1 Score: 123.25 bits (308), Expect = 8.425e-28
Identity = 61/174 (35.06%), Postives = 104/174 (59.77%), Query Frame = 2

Query:   11 GRGTFTKEETIRLAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPPS 532
            G    + + + +L ++E+   + MFV LS++WLD+ E L KL I+  G+      P+ F+  GNF S P     +   +L+     L  II   P + ++SRF+F+PGP+D G  + LP   L + +T E ++ +P ++F++NPCR+++ TQE+  FR+DL+ +M R+C+  PS
Sbjct:  263 GPSNTSVKTSAKLKQLEEENKDAMFVFLSDVWLDQVEVLEKLRIMFAGYSPAP--PTCFILCGNFSSAP--YGKNQVQALKDSLKTLADIICEYPDIHQSSRFVFVPGPEDPGFGSILPRPPLAESITNEFRQRVPFSVFTTNPCRIQYCTQEITVFREDLVNKMCRNCVRFPS 432

[Close]

BLAST of HO664308 vs. SwissProt
Match: DPOE2_BOVIN (DNA polymerase epsilon subunit 2 OS=Bos taurus GN=POLE2 PE=2 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 8.425e-28
Identity = 59/174 (33.91%), Postives = 103/174 (59.20%), Query Frame = 2

Query:   11 GRGTFTKEETIRLAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPPS 532
            G    + + + +L ++E    + MFV +S++WLD+ E L KL I+  G+      P+ F+  GNF S P     +   +L+     L  II   P + ++SRF+F+PGP+D G  + LP     + +T E ++ +P ++F++NPCR+++ TQE++ FR+DL+ +M R+C+  PS
Sbjct:  263 GPSNTSVKTSAKLKQLEDENKDAMFVFISDVWLDQVEVLEKLHIMFSGYSPAP--PTCFILCGNFSSAP--YGKNQVQALKDSLKTLADIICGYPNIHQSSRFVFVPGPEDPGFGSILPRPPFAESITNEFRQRVPFSVFTTNPCRIQYCTQEIIVFREDLVNKMCRNCVRFPS 432

[Close]

BLAST of HO664308 vs. SwissProt
Match: DPOE2_DICDI (DNA polymerase epsilon subunit 2 OS=Dictyostelium discoideum GN=pole2 PE=3 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.805e-23
Identity = 53/170 (31.18%), Postives = 94/170 (55.29%), Query Frame = 2

Query:   29 KEETIRLAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPS--TALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPPS 532
            K+   +L + EK   ++  + LS++WLD E  + +L+ +  G++  +  P   + MGNF   P  L N     L+  F +L  +I   P + + ++F+F+PGP D   S    LP   +     ++    IP++ F++NPCR+++ +QE++ FR DL  +MRR C++ PS
Sbjct:  254 KKTMEQLIKYEKEKEDNSILFLSDVWLDSERVMERLDYLFGGYK--DCPPFAMILMGNFTEHP--LINGTQYQLKKYFNQLAMVIQKYPNIHQFTQFIFVPGPTDPTGSLLNILPKFPISNVFIKDFISLIPKSTFTTNPCRIRYCSQEIIIFRDDLTNKMRRHCILEPS 419

[Close]

BLAST of HO664308 vs. SwissProt
Match: DPB2_CRYNE (DNA polymerase epsilon subunit B OS=Cryptococcus neoformans GN=DPB2 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 1.702e-20
Identity = 54/148 (36.49%), Postives = 80/148 (54.05%), Query Frame = 2

Query:   83 FVILSEIWLDKEEALGKLEIVLDGF-ESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLM 523
            FVILS++WLD    +  L  + +G+  + E  P +FV  GNFC                 F  L +++ + P L  +S F+F+PGP D   ST LP  +LP   T  L   IP A F SNPCR+K++  E+V  R+DL+ +M R+ ++
Sbjct:  291 FVILSDVWLDHPRTMPALRQMFEGYANTAEYRPMVFVLCGNFCQGGWE-GQEGLKRYSRGFNSLAELLQSIPLL-HSSHFVFVPGPSDPWSSTTLPRPSLPSAFTTRLSNRIPNARFVSNPCRLKYFGMEIVICREDLMGKMMRNLVV 436

[Close]

BLAST of HO664308 vs. SwissProt
Match: DPB2_SCHPO (DNA polymerase epsilon subunit B OS=Schizosaccharomyces pombe GN=dpb2 PE=1 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.548e-18
Identity = 48/161 (29.81%), Postives = 85/161 (52.80%), Query Frame = 2

Query:   47 LAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTAL---PMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCL 520
            L   E+   +  FV +SE+ LD  +    LE +   +ES E VP   +  G+F S   + + S     +  F +L   +   P++ E ++F+F+PGP+D   +  +   P  ++P +    +Q+     IF+SNPCR+ F++QEV+ +R D+  R +R+ L
Sbjct:  320 LRHAEQAYQSQAFVCISEVHLDNHQTFYALEKIFQKYESSEAVPFCIILCGSFMSSAFHNSGSSIQ-YKEGFNKLAASLEKFPKICEKTKFIFVPGPNDPWTTNGISLMPKHSIPLHFVNRIQRVCKHTIFASNPCRIIFFSQEVLIYRDDISGRFQRNSL 479

[Close]

BLAST of HO664308 vs. SwissProt
Match: DPOE2_CAEEL (Probable DNA polymerase epsilon subunit 2 OS=Caenorhabditis elegans GN=pole-2 PE=2 SV=2)

HSP 1 Score: 83.5741 bits (205), Expect = 7.398e-16
Identity = 52/156 (33.33%), Postives = 81/156 (51.92%), Query Frame = 2

Query:   44 RLAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRR 511
            RL     +  +   V LS+++LD ++ +  +  +L G++  +  P   VF GNFCS+P      D   L   F  L   +    +  E ++F+F+PGPDD    T LP   LP  L + +   I    F+SNPCR++F +QE+V FR DL+ +  R
Sbjct:  263 RLRSALAKQEDTSLVFLSDVFLDDKKVMKAVFKLLQGYK--DQPPVAIVFCGNFCSRPRQTDTIDL--LDRGFRWLANQLTPLRKDYEKTQFIFVPGPDDPFVDTVLPRPHLPSLLFKHISPII-SCTFASNPCRIQFASQEIVVFRSDLIKKTCR 413

[Close]

BLAST of HO664308 vs. SwissProt
Match: DPB2_YEAST (DNA polymerase epsilon subunit B OS=Saccharomyces cerevisiae GN=DPB2 PE=1 SV=3)

HSP 1 Score: 81.6481 bits (200), Expect = 2.811e-15
Identity = 56/180 (31.11%), Postives = 93/180 (51.67%), Query Frame = 2

Query:   29 KEETIRLAEMEKRAVNDMFVIL-SEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKP--CNLANSDYSS---LRLQFGELGQIIAARPRLKENSRFLFIPGPDDA-------GPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPP 529
            K+  IRL  +EK   +  FVIL + ++LD  + +  L  +L      +  P+L ++ G+F S P   ++++ + SS    +  F  L  +++    L EN+  +FIPGP+D        G S  LP   +P   T+++ K     ++SSNP R+ + +QE+V FR DL  R +R  L  P
Sbjct:  381 KDLKIRLHLLEKELTDHKFVILGANLFLDDLKIMTALSKILQKLN--DDPPTLLIWQGSFTSVPVFASMSSRNISSSTQFKNNFDALATLLSRFDNLTENTTMIFIPGPNDLWGSMVSLGASGTLPQDPIPSAFTKKINKVCKNVVWSSNPTRIAYLSQEIVIFRDDLSGRFKRHRLEFP 558

[Close]

BLAST of HO664308 vs. SwissProt
Match: DPB2_ASHGO (DNA polymerase epsilon subunit B OS=Ashbya gossypii GN=DPB2 PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 2.015e-13
Identity = 57/187 (30.48%), Postives = 92/187 (49.20%), Query Frame = 2

Query:   29 KEETIRLAEMEKRAVNDMFVILS-EIWLDK---EEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPC-----NLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTA-------LPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPPSVTE 541
            KE  IRL  +EK   +  FV L   I+LD    E AL KL  VL+        P++ V  G+F S P      + ++S  ++ R  F  L ++++   RL   + F+FIPG +D   S A       LP   +P     ++ +     ++ SNP R+ + +QE+V  R D+  R +R+ ++ P+V E
Sbjct:  365 KELKIRLHYLEKELTDHRFVFLGGNIFLDDTMTETALAKLFDVLE-----HDPPTVLVLPGSFTSTPIYPSSDSKSSSSTAAYRANFDALAKLLSKYERLINETTFVFIPGDNDPWGSMAYLGVAGTLPQHPIPGDFVTKVNRICKHVVWGSNPTRIAYLSQELVIMRDDMCNRFKRNSIIFPTVEE 546

[Close]

BLAST of HO664308 vs. TrEMBL
Match: B9I4W5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_806207 PE=4 SV=1)

HSP 1 Score: 303.138 bits (775), Expect = 7.932e-81
Identity = 144/183 (78.69%), Postives = 162/183 (88.52%), Query Frame = 2

Query:   11 GRGTFTKEETIRLAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPPSVTETADPFQ 559
            G GT TKEET+RLAE+EKR++NDMFVILS+IW+D EEA+GKLE VLDGFES EVVPSLFVFMGNFCS PCNL+   +SSLR QFG+LGQ+I A  RLKE+SRF+FIPGP+DAGPST LP C LPKYLTEELQK+IP AIF+SNPCRVKFYTQE+VFFR DLLYRMRRSCLMPPS  ET DPF+
Sbjct:  252 GGGTLTKEETLRLAELEKRSLNDMFVILSDIWVDSEEAMGKLETVLDGFESQEVVPSLFVFMGNFCSHPCNLSFHSFSSLRSQFGKLGQMIGAHQRLKEHSRFMFIPGPEDAGPSTVLPRCPLPKYLTEELQKHIPNAIFASNPCRVKFYTQEIVFFRHDLLYRMRRSCLMPPSTEETDDPFE 434

[Close]

BLAST of HO664308 vs. TrEMBL
Match: D7SIP4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_0.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00008158001 PE=4 SV=1)

HSP 1 Score: 301.597 bits (771), Expect = 2.308e-80
Identity = 140/183 (76.50%), Postives = 163/183 (89.07%), Query Frame = 2

Query:   11 GRGTFTKEETIRLAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPPSVTETADPFQ 559
            G GT T+EET+RLA +EK+AVNDMFVILS++WLD EE +GKL  VLDG+E+VEVVPSLF+FMGNFCS PCNL+   +SSLRLQFG+LGQ+IA+ PRLKE+S+FLFIPGPDDAGPST LP CALP YLTEE +KYIP AIFSSNPCR+KFYTQE+V FRQDLLYRMRRSCL+PPS  ET+DPF+
Sbjct:  261 GGGTLTREETLRLAGLEKKAVNDMFVILSDVWLDNEETMGKLATVLDGYENVEVVPSLFIFMGNFCSHPCNLSFHSFSSLRLQFGKLGQMIASHPRLKEHSQFLFIPGPDDAGPSTVLPRCALPNYLTEEFKKYIPNAIFSSNPCRIKFYTQEIVLFRQDLLYRMRRSCLVPPSPEETSDPFE 443

[Close]

BLAST of HO664308 vs. TrEMBL
Match: B9SYF4_RICCO (DNA polymerase epsilon subunit B, putative OS=Ricinus communis GN=RCOM_0119080 PE=4 SV=1)

HSP 1 Score: 299.286 bits (765), Expect = 1.145e-79
Identity = 143/183 (78.14%), Postives = 162/183 (88.52%), Query Frame = 2

Query:   11 GRGTFTKEETIRLAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPPSVTETADPFQ 559
            G G+ TKEET+ L E+E +AVNDMFVILS+IWLD EEA+GKLE+VLDGFE  EVVPSLFVFMGNFCS PCNL+   +S+LRLQFG+LG++IAA  RLKE+SRFLFIPGPDDAGPST LP CALPKYLTE+LQK+IP AIFS+NPCRVKFYTQE+VFFR DLLYRM RSCLMPPS  ETADPF+
Sbjct:  270 GGGSLTKEETLGLEELECKAVNDMFVILSDIWLDSEEAMGKLEMVLDGFEIQEVVPSLFVFMGNFCSHPCNLSFHSFSTLRLQFGKLGKMIAAHQRLKEHSRFLFIPGPDDAGPSTVLPRCALPKYLTEDLQKHIPNAIFSTNPCRVKFYTQEIVFFRHDLLYRMHRSCLMPPSTEETADPFE 452

[Close]

BLAST of HO664308 vs. TrEMBL
Match: Q9C577_ARATH (DNA polymerase epsilon subunit B-like protein OS=Arabidopsis thaliana GN=At5g22110 PE=4 SV=1)

HSP 1 Score: 290.812 bits (743), Expect = 4.074e-77
Identity = 140/183 (76.50%), Postives = 157/183 (85.79%), Query Frame = 2

Query:   11 GRGTFTKEETIRLAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPPSVTETADPFQ 559
            G GT TKEE I+LA++E++AVND FVILS+IWLD EE + KLE VLDGFESVE VPSLFVFMGNFCS+PCNL+   YSSLR QFG+LG++I   PRLKENSRFLFIPGP+DAGPST LP CALPKYLTEEL+  IP AIFSSNPCRVKFY QE+VFFRQDLLYRMRRSCL+ PS  ET DPF+
Sbjct:  221 GGGTLTKEEMIKLADLERQAVNDTFVILSDIWLDDEEVMRKLETVLDGFESVETVPSLFVFMGNFCSRPCNLSFGSYSSLREQFGKLGRMIGNHPRLKENSRFLFIPGPEDAGPSTVLPRCALPKYLTEELRNIIPNAIFSSNPCRVKFYNQEIVFFRQDLLYRMRRSCLVTPSSEETNDPFK 403

[Close]

BLAST of HO664308 vs. TrEMBL
Match: Q56XM7_ARATH (DNA polymerase epsilon subunit B-like protein OS=Arabidopsis thaliana GN=At5g22110 PE=2 SV=1)

HSP 1 Score: 290.812 bits (743), Expect = 4.074e-77
Identity = 140/183 (76.50%), Postives = 157/183 (85.79%), Query Frame = 2

Query:   11 GRGTFTKEETIRLAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPPSVTETADPFQ 559
            G GT TKEE I+LA++E++AVND FVILS+IWLD EE + KLE VLDGFESVE VPSLFVFMGNFCS+PCNL+   YSSLR QFG+LG++I   PRLKENSRFLFIPGP+DAGPST LP CALPKYLTEEL+  IP AIFSSNPCRVKFY QE+VFFRQDLLYRMRRSCL+ PS  ET DPF+
Sbjct:  260 GGGTLTKEEMIKLADLERQAVNDTFVILSDIWLDDEEVMRKLETVLDGFESVETVPSLFVFMGNFCSRPCNLSFGSYSSLREQFGKLGRMIGNHPRLKENSRFLFIPGPEDAGPSTVLPRCALPKYLTEELRNIIPNAIFSSNPCRVKFYNQEIVFFRQDLLYRMRRSCLVTPSSEETNDPFK 442

[Close]

BLAST of HO664308 vs. TrEMBL
Match: Q500V9_ARATH (At5g22110 OS=Arabidopsis thaliana GN=At5g22110 PE=2 SV=1)

HSP 1 Score: 290.812 bits (743), Expect = 4.074e-77
Identity = 140/183 (76.50%), Postives = 157/183 (85.79%), Query Frame = 2

Query:   11 GRGTFTKEETIRLAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPPSVTETADPFQ 559
            G GT TKEE I+LA++E++AVND FVILS+IWLD EE + KLE VLDGFESVE VPSLFVFMGNFCS+PCNL+   YSSLR QFG+LG++I   PRLKENSRFLFIPGP+DAGPST LP CALPKYLTEEL+  IP AIFSSNPCRVKFY QE+VFFRQDLLYRMRRSCL+ PS  ET DPF+
Sbjct:  260 GGGTLTKEEMIKLADLERQAVNDTFVILSDIWLDDEEVMRKLETVLDGFESVETVPSLFVFMGNFCSRPCNLSFGSYSSLREQFGKLGRMIGNHPRLKENSRFLFIPGPEDAGPSTVLPRCALPKYLTEELRNIIPNAIFSSNPCRVKFYNQEIVFFRQDLLYRMRRSCLVTPSSEETNDPFK 442

[Close]

BLAST of HO664308 vs. TrEMBL
Match: D7M0T9_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_489063 PE=4 SV=1)

HSP 1 Score: 290.426 bits (742), Expect = 5.321e-77
Identity = 140/183 (76.50%), Postives = 156/183 (85.25%), Query Frame = 2

Query:   11 GRGTFTKEETIRLAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPPSVTETADPFQ 559
            G GT TKEETIRLA++E++AV+D FVILS+IWLD EE + KLE VLDGFESVE VPSLFVFMGNFCS+PCNL+   YSSLR QFG+LG++I   PRLKENSRFLFIPGP DAGPST LP CALP YLTEEL+  IP AIFSSNPCRVKFY QE+VFFRQDLLYRMRRSCL+ PS  ET DPF+
Sbjct:  260 GGGTLTKEETIRLADLERQAVSDTFVILSDIWLDDEEVIRKLETVLDGFESVETVPSLFVFMGNFCSRPCNLSFGSYSSLREQFGKLGRMIGNHPRLKENSRFLFIPGPGDAGPSTVLPRCALPNYLTEELRNIIPNAIFSSNPCRVKFYNQEIVFFRQDLLYRMRRSCLVAPSTEETKDPFK 442

[Close]

BLAST of HO664308 vs. TrEMBL
Match: Q6ATB7_ORYSJ (Os05g0160800 protein OS=Oryza sativa subsp. japonica GN=OSJNBa0034O12.11 PE=4 SV=1)

HSP 1 Score: 288.115 bits (736), Expect = 2.641e-76
Identity = 134/183 (73.22%), Postives = 159/183 (86.89%), Query Frame = 2

Query:   11 GRGTFTKEETIRLAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPPSVTETADPFQ 559
            G G    EET+RL+ +EK+A+NDMFVILS++WLD  E + KL +VLDG++S+E VPSLFV MGNFCS+PCNLA + +  LRLQFG+LG++IAAR RLKE+SRFLFIPGPDDAGPS ALP CALPKYLTEELQK+IP AIF SNPCRVKFYTQE+VFFRQDLLYRMRRSCL+PP+  ET+DPF+
Sbjct:  104 GGGVIPTEETVRLSTLEKKAMNDMFVILSDVWLDNYETMEKLGVVLDGYDSLEAVPSLFVLMGNFCSRPCNLAFNSFEELRLQFGKLGEMIAARSRLKEHSRFLFIPGPDDAGPSKALPRCALPKYLTEELQKHIPNAIFVSNPCRVKFYTQEIVFFRQDLLYRMRRSCLIPPTTEETSDPFE 286

[Close]

BLAST of HO664308 vs. TrEMBL
Match: B9FML5_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_17214 PE=4 SV=1)

HSP 1 Score: 288.115 bits (736), Expect = 2.641e-76
Identity = 134/183 (73.22%), Postives = 159/183 (86.89%), Query Frame = 2

Query:   11 GRGTFTKEETIRLAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPPSVTETADPFQ 559
            G G    EET+RL+ +EK+A+NDMFVILS++WLD  E + KL +VLDG++S+E VPSLFV MGNFCS+PCNLA + +  LRLQFG+LG++IAAR RLKE+SRFLFIPGPDDAGPS ALP CALPKYLTEELQK+IP AIF SNPCRVKFYTQE+VFFRQDLLYRMRRSCL+PP+  ET+DPF+
Sbjct:  265 GGGVIPTEETVRLSTLEKKAMNDMFVILSDVWLDNYETMEKLGVVLDGYDSLEAVPSLFVLMGNFCSRPCNLAFNSFEELRLQFGKLGEMIAARSRLKEHSRFLFIPGPDDAGPSKALPRCALPKYLTEELQKHIPNAIFVSNPCRVKFYTQEIVFFRQDLLYRMRRSCLIPPTTEETSDPFE 447

[Close]

BLAST of HO664308 vs. TrEMBL
Match: B8AYE7_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_18564 PE=4 SV=1)

HSP 1 Score: 288.115 bits (736), Expect = 2.641e-76
Identity = 134/183 (73.22%), Postives = 159/183 (86.89%), Query Frame = 2

Query:   11 GRGTFTKEETIRLAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPPSVTETADPFQ 559
            G G    EET+RL+ +EK+A+NDMFVILS++WLD  E + KL +VLDG++S+E VPSLFV MGNFCS+PCNLA + +  LRLQFG+LG++IAAR RLKE+SRFLFIPGPDDAGPS ALP CALPKYLTEELQK+IP AIF SNPCRVKFYTQE+VFFRQDLLYRMRRSCL+PP+  ET+DPF+
Sbjct:  265 GGGVIPTEETVRLSTLEKKAMNDMFVILSDVWLDNYETMEKLGVVLDGYDSLEAVPSLFVLMGNFCSRPCNLAFNSFEELRLQFGKLGEMIAARSRLKEHSRFLFIPGPDDAGPSKALPRCALPKYLTEELQKHIPNAIFVSNPCRVKFYTQEIVFFRQDLLYRMRRSCLIPPTTEETSDPFE 447

[Close]

BLAST of HO664308 vs. TAIR peptide
Match: AT5G22110.1 (| Symbols: ATDPB2, CYL2, DPB2 | DNA polymerase epsilon subunit B2 | chr5:7331071-7334178 REVERSE LENGTH=526)

HSP 1 Score: 290.812 bits (743), Expect = 3.267e-79
Identity = 140/183 (76.50%), Postives = 157/183 (85.79%), Query Frame = 2

Query:   11 GRGTFTKEETIRLAEMEKRAVNDMFVILSEIWLDKEEALGKLEIVLDGFESVEVVPSLFVFMGNFCSKPCNLANSDYSSLRLQFGELGQIIAARPRLKENSRFLFIPGPDDAGPSTALPMCALPKYLTEELQKYIPEAIFSSNPCRVKFYTQEVVFFRQDLLYRMRRSCLMPPSVTETADPFQ 559
            G GT TKEE I+LA++E++AVND FVILS+IWLD EE + KLE VLDGFESVE VPSLFVFMGNFCS+PCNL+   YSSLR QFG+LG++I   PRLKENSRFLFIPGP+DAGPST LP CALPKYLTEEL+  IP AIFSSNPCRVKFY QE+VFFRQDLLYRMRRSCL+ PS  ET DPF+
Sbjct:  260 GGGTLTKEEMIKLADLERQAVNDTFVILSDIWLDDEEVMRKLETVLDGFESVETVPSLFVFMGNFCSRPCNLSFGSYSSLREQFGKLGRMIGNHPRLKENSRFLFIPGPEDAGPSTVLPRCALPKYLTEELRNIIPNAIFSSNPCRVKFYNQEIVFFRQDLLYRMRRSCLVTPSSEETNDPFK 442

The following BLAST results are available for this feature:

BLAST of HO664308 vs. SwissProt
BLAST of HO664308 vs. TrEMBL
BLAST of HO664308 vs. TAIR peptide

BLAST of HO664308 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
DPOE2_CHICK	6.234e-31	37.36	DNA polymerase epsilon subunit 2 OS=Gallus gallus ...	[more]
DPOE2_MOUSE	8.425e-28	34.97	DNA polymerase epsilon subunit 2 OS=Mus musculus G...	[more]
DPOE2_HUMAN	8.425e-28	35.06	DNA polymerase epsilon subunit 2 OS=Homo sapiens G...	[more]
DPOE2_BOVIN	8.425e-28	33.91	DNA polymerase epsilon subunit 2 OS=Bos taurus GN=...	[more]
DPOE2_DICDI	2.805e-23	31.18	DNA polymerase epsilon subunit 2 OS=Dictyostelium ...	[more]
DPB2_CRYNE	1.702e-20	36.49	DNA polymerase epsilon subunit B OS=Cryptococcus n...	[more]
DPB2_SCHPO	3.548e-18	29.81	DNA polymerase epsilon subunit B OS=Schizosaccharo...	[more]
DPOE2_CAEEL	7.398e-16	33.33	Probable DNA polymerase epsilon subunit 2 OS=Caeno...	[more]
DPB2_YEAST	2.811e-15	31.11	DNA polymerase epsilon subunit B OS=Saccharomyces ...	[more]
DPB2_ASHGO	2.015e-13	30.48	DNA polymerase epsilon subunit B OS=Ashbya gossypi...	[more]

BLAST of HO664308 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9I4W5_POPTR	7.932e-81	78.69	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7SIP4_VITVI	2.308e-80	76.50	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9SYF4_RICCO	1.145e-79	78.14	DNA polymerase epsilon subunit B, putative OS=Rici...	[more]
Q9C577_ARATH	4.074e-77	76.50	DNA polymerase epsilon subunit B-like protein OS=A...	[more]
Q56XM7_ARATH	4.074e-77	76.50	DNA polymerase epsilon subunit B-like protein OS=A...	[more]
Q500V9_ARATH	4.074e-77	76.50	At5g22110 OS=Arabidopsis thaliana GN=At5g22110 PE=...	[more]
D7M0T9_ARALY	5.321e-77	76.50	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q6ATB7_ORYSJ	2.641e-76	73.22	Os05g0160800 protein OS=Oryza sativa subsp. japoni...	[more]
B9FML5_ORYSJ	2.641e-76	73.22	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B8AYE7_ORYSI	2.641e-76	73.22	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of HO664308 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT5G22110.1	3.267e-79	76.50	\| Symbols: ATDPB2, CYL2, DPB2 \| DNA polymerase eps...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007185	DNA polymerase alpha/epsilon, subunit B	PFAM	PF04042	DNA_pol_E_B	coord: 28..177 score: 4.3
None	No IPR available	PANTHER	PTHR12708	DNA POLYMERASE EPSILON SUBUNIT B	coord: 11..183 score: 1.9

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO664308 ID=HO664308; Name=HO664308; organism=Cicer arietinum; type=EST; length=559bp
GCGTGGTCGCGGCCGAGGTACTTTTACAAAAGAGGAAACGATCAGACTGG
CAGAGATGGAGAAAAGAGCAGTCAATGACATGTTTGTCATACTATCTGAA
ATTTGGCTAGACAAGGAAGAGGCTTTGGGAAAGCTAGAGATTGTTCTTGA
TGGTTTTGAGAGTGTAGAAGTGGTTCCTTCCTTATTTGTTTTCATGGGAA
ACTTCTGTTCTAAACCATGTAACCTTGCAAATTCCGATTATTCGAGCCTA
AGGTTGCAGTTTGGCGAGCTAGGGCAAATTATTGCTGCCCGTCCACGGCT
AAAAGAGAACAGCCGATTTCTTTTTATTCCAGGTCCTGATGATGCAGGTC
CTTCAACAGCTCTTCCTATGTGTGCTCTACCTAAATATTTAACTGAGGAG
CTTCAAAAGTATATTCCCGAAGCCATATTTTCAAGCAATCCCTGCAGGGT
AAAATTCTATACCCAAGAAGTTGTATTTTTCCGCCAGGATCTGCTGTATA
GAATGCGTCGTTCTTGTTTAATGCCGCCTTCTGTGACAGAAACTGCAGAT
CCTTTTCAG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006260	DNA replication

Vocabulary: **INTERPRO**
Term	Definition
IPR007185	DNA_pol_a/d/e_bsu

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