FE669306

EST Overview


Name	FE669306
Unique Name	FE669306
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	463

Libraries


Library Name	Type
LIBEST_022821 Chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of FE669306 vs. SwissProt
Match: HSP7S_PEA (Stromal 70 kDa heat shock-related protein, chloroplastic OS=Pisum sativum GN=HSP70 PE=2 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 1.147e-35
Identity = 75/90 (83.33%), Postives = 80/90 (88.89%), Query Frame = 3

Query:  192 QIHGLGTASFSSLKKPNSTSSNSRTLFLGHRLNSNHVSFPRAAFPKLRAKTLRKGFTLRVVNEKVVGIDLGTTNSAVAAMEGRKPTIITN 461
            QIHGLGTASFSSLKKP+S S NS+TLF G RLNSNH  F RAAFPKL +KT +KGFTLRVV+EKVVGIDLGTTNSAVAAMEG KPTIITN
Sbjct:    6 QIHGLGTASFSSLKKPSSISGNSKTLFFGQRLNSNHSPFTRAAFPKLSSKTFKKGFTLRVVSEKVVGIDLGTTNSAVAAMEGGKPTIITN 95

[Close]

BLAST of FE669306 vs. SwissProt
Match: HSP7S_CUCMA (Stromal 70 kDa heat shock-related protein, chloroplastic (Fragment) OS=Cucurbita maxima PE=1 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 6.561e-7
Identity = 25/28 (89.29%), Postives = 27/28 (96.43%), Query Frame = 3

Query:  378 EKVVGIDLGTTNSAVAAMEGRKPTIITN 461
            +KVVGIDLGTTNSAVAAMEG KPTI+TN
Sbjct:    1 QKVVGIDLGTTNSAVAAMEGGKPTIVTN 28

[Close]

BLAST of FE669306 vs. TrEMBL
Match: Q1SKX2_MEDTR (Chaperone DnaK OS=Medicago truncatula GN=MtrDRAFT_AC140550g11v2 PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 1.915e-28
Identity = 71/94 (75.53%), Postives = 77/94 (81.91%), Query Frame = 3

Query:  192 QIHGLGTASFSSLKKPNSTSSNS----RTLFLGHRLNSNHVSFPRAAFPKLRAKTLRKGFTLRVVNEKVVGIDLGTTNSAVAAMEGRKPTIITN 461
            QIHGLG   FS L+KP+S++SNS    +TLF GHRLNSNH +FPRAAFPKL   T RK FT RVVNEKVVGIDLGTTNSAVAAMEG KPTIITN
Sbjct:    8 QIHGLG---FSPLRKPSSSTSNSSSYSKTLFFGHRLNSNHATFPRAAFPKL--STNRKPFTFRVVNEKVVGIDLGTTNSAVAAMEGGKPTIITN 96

[Close]

BLAST of FE669306 vs. TrEMBL
Match: Q39641_CUCSA (Heat shock protein 70 OS=Cucumis sativus GN=hsp70 PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.122e-19
Identity = 54/93 (58.06%), Postives = 67/93 (72.04%), Query Frame = 3

Query:  192 QIHGLGTASFSSLKKPNSTSSNSRTLFLGHRLNSNHVSFPRAAFPKLRAKTLRKGFT---LRVVNEKVVGIDLGTTNSAVAAMEGRKPTIITN 461
            QIHGLG  SF++     S + +SR++F G +L  N  +FP AAF  LR+ T R+  +   LR+VNEKVVGIDLGTTNSAVAAMEG KPTI+TN
Sbjct:    7 QIHGLGAPSFAAASMRKSNNVSSRSVFFGQKLG-NSSAFPAAAFLNLRSNTSRRNSSVRPLRIVNEKVVGIDLGTTNSAVAAMEGGKPTIVTN 98

[Close]

BLAST of FE669306 vs. TrEMBL
Match: A2TJV6_CUCSA (Chloroplast HSP70 OS=Cucumis sativus PE=3 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.122e-19
Identity = 54/93 (58.06%), Postives = 67/93 (72.04%), Query Frame = 3

Query:  192 QIHGLGTASFSSLKKPNSTSSNSRTLFLGHRLNSNHVSFPRAAFPKLRAKTLRKGFT---LRVVNEKVVGIDLGTTNSAVAAMEGRKPTIITN 461
            QIHGLG  SF++     S + +SR++F G +L  N  +FP AAF  LR+ T R+  +   LR+VNEKVVGIDLGTTNSAVAAMEG KPTI+TN
Sbjct:    7 QIHGLGAPSFAAASMRKSNNVSSRSVFFGQKLG-NSSAFPAAAFLNLRSNTSRRNSSVRPLRIVNEKVVGIDLGTTNSAVAAMEGGKPTIVTN 98

[Close]

BLAST of FE669306 vs. TrEMBL
Match: O04056_CITLA (Heat shock protein 70 OS=Citrullus lanatus PE=2 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.727e-19
Identity = 54/93 (58.06%), Postives = 65/93 (69.89%), Query Frame = 3

Query:  192 QIHGLGTASFSSLKKPNSTSSNSRTLFLGHRLNSNHVSFPRAAFPKLRAKTLRKGFT---LRVVNEKVVGIDLGTTNSAVAAMEGRKPTIITN 461
            QIHGLG  SF++     S   +SRT+F G +L  N  +FP A F KLR+   R+  +   LR+VNEKVVGIDLGTTNSAVAAMEG KPTI+TN
Sbjct:    7 QIHGLGAPSFAAASMRKSNHVSSRTVFFGQKLG-NSSAFPTATFLKLRSNISRRNSSVRPLRIVNEKVVGIDLGTTNSAVAAMEGGKPTIVTN 98

[Close]

BLAST of FE669306 vs. TrEMBL
Match: A5BEE7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_000728 PE=3 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.727e-19
Identity = 60/95 (63.16%), Postives = 68/95 (71.58%), Query Frame = 3

Query:  192 QIHGLGTASF-SSLKKPNSTSSNSRTLFLGHRLNSNHVSF--PRAAFPKLRAKTLRKGFT--LRVVNEKVVGIDLGTTNSAVAAMEGRKPTIITN 461
            QIHGLGT  F SS  KP  TSS  RT+F G RL+ +  SF  P+ AF KL+  + R   T  +RVVNEKVVGIDLGTTNSAVAAMEG KPTI+TN
Sbjct:    7 QIHGLGTTGFASSSAKPKRTSS--RTVFFGQRLSPSTASFGLPKTAFLKLKRNSRRDYSTGPVRVVNEKVVGIDLGTTNSAVAAMEGGKPTIVTN 99

[Close]

BLAST of FE669306 vs. TrEMBL
Match: B9SKC7_RICCO (Heat shock protein, putative OS=Ricinus communis GN=RCOM_0759010 PE=3 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.740e-13
Identity = 52/92 (56.52%), Postives = 58/92 (63.04%), Query Frame = 3

Query:  192 QIHGLGTASFSSLK--KPNSTSSNSRTLFLGHRLNSNHVSFPRAAFPKLRAKTLRKGFTLRVVNEKVVGIDLGTTNSAVAAMEGRKPTIITN 461
            QIH LG   F S K  KP S S   +T+FLG  L S+  +   A   +      RK   LRVVNEKVVGIDLGTTNSAVAAMEG KPTI+TN
Sbjct:    7 QIHVLGGIGFPSSKSRKP-SFSFPKKTVFLGSNLKSHQTA---AFLRQSNTNARRKYGPLRVVNEKVVGIDLGTTNSAVAAMEGGKPTIVTN 94

[Close]

BLAST of FE669306 vs. TrEMBL
Match: O50036_SPIOL (Heat shock 70 protein OS=Spinacia oleracea GN=HSC70-9 PE=2 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.128e-12
Identity = 48/98 (48.98%), Postives = 66/98 (67.35%), Query Frame = 3

Query:  192 QIHGLGTASFS-SLKKPNSTSSNSRTLFLGHRLNSNH--VSFPRAAFPKLRAKTLRKGFT-----LRVVNEKVVGIDLGTTNSAVAAMEGRKPTIITN 461
            QIH LG   F+ S  KP+S+  NS  +F G +++ +   +  P++AF +L+  +  +  +     +RVVNEKVVGIDLGTTNSAVAAMEG KPTI+TN
Sbjct:    7 QIHVLGATPFTTSSSKPSSSRPNS--VFFGQKMSPSTACIGNPKSAFLRLKKSSGGRRRSAGVGPVRVVNEKVVGIDLGTTNSAVAAMEGGKPTIVTN 102

[Close]

BLAST of FE669306 vs. TrEMBL
Match: D7MPG3_ARALY (Cphsc70-2 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_495046 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.473e-12
Identity = 50/96 (52.08%), Postives = 63/96 (65.62%), Query Frame = 3

Query:  192 QIHGLGTASFSSLKK--PNSTSSNSRTLFLGHRLNSNHVSFPRAAFPKL-RAKTLRKGFTL---RVVNEKVVGIDLGTTNSAVAAMEGRKPTIITN 461
            QIH LG   F +  K   N T+S  R++F G+R  ++  S P +AF ++ R       +T+   RVVNEKVVGIDLGTTNSAVAAMEG KPTI+TN
Sbjct:    7 QIHILGGIGFPASGKNLDNKTNSIPRSVFFGNR--TSPFSTPTSAFLRMGRRNNNASRYTVGPVRVVNEKVVGIDLGTTNSAVAAMEGGKPTIVTN 100

[Close]

BLAST of FE669306 vs. TrEMBL
Match: Q9M637_ARATH (Heat shock protein 70 OS=Arabidopsis thaliana GN=Hsc70-7 PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 5.596e-12
Identity = 50/101 (49.50%), Postives = 63/101 (62.38%), Query Frame = 3

Query:  192 QIHGLGTASF-------SSLKKPNSTSSNSRTLFLGHRLNSNHVSFPRAAFPKL-RAKTLRKGFTL---RVVNEKVVGIDLGTTNSAVAAMEGRKPTIITN 461
            QIH LG   F       S+    N T+S  R++F G+R  ++  S P +AF ++ R       +T+   RVVNEKVVGIDLGTTNSAVAAMEG KPTI+TN
Sbjct:    7 QIHILGGIGFPNSFCSSSTKNLDNKTNSIPRSVFFGNR--TSPFSTPTSAFLRMGRRNNNASRYTVGPVRVVNEKVVGIDLGTTNSAVAAMEGGKPTIVTN 105

[Close]

BLAST of FE669306 vs. TrEMBL
Match: Q9LTX9_ARATH (Heat shock protein 70 OS=Arabidopsis thaliana GN=At5g49910 PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 5.596e-12
Identity = 52/101 (51.49%), Postives = 66/101 (65.35%), Query Frame = 3

Query:  192 QIHGLG-----TASFSSLKK--PNSTSSNSRTLFLGHRLNSNHVSFPRAAFPKL-RAKTLRKGFTL---RVVNEKVVGIDLGTTNSAVAAMEGRKPTIITN 461
            QIH LG     T+S SS  K   N T+S  R++F G+R  ++  + P +AF ++ R       +T+   RVVNEKVVGIDLGTTNSAVAAMEG KPTI+TN
Sbjct:    7 QIHILGGIGFPTSSSSSSTKNLDNKTNSIPRSVFFGNR--TSPFTTPTSAFLRMGRRNNNASRYTVGPVRVVNEKVVGIDLGTTNSAVAAMEGGKPTIVTN 105

[Close]

BLAST of FE669306 vs. TAIR peptide
Match: AT5G49910.1 (| Symbols: CPHSC70-2EAT SHOCK PROTEIN 70-2, HSC70-7, cpHsc70-2 | chloroplast heat shock protein 70-2 | chr5:20303470-20306295 FORWARD LENGTH=718)

HSP 1 Score: 73.9442 bits (180), Expect = 4.188e-14
Identity = 52/101 (51.49%), Postives = 66/101 (65.35%), Query Frame = 3

Query:  192 QIHGLG-----TASFSSLKK--PNSTSSNSRTLFLGHRLNSNHVSFPRAAFPKL-RAKTLRKGFTL---RVVNEKVVGIDLGTTNSAVAAMEGRKPTIITN 461
            QIH LG     T+S SS  K   N T+S  R++F G+R  ++  + P +AF ++ R       +T+   RVVNEKVVGIDLGTTNSAVAAMEG KPTI+TN
Sbjct:    7 QIHILGGIGFPTSSSSSSTKNLDNKTNSIPRSVFFGNR--TSPFTTPTSAFLRMGRRNNNASRYTVGPVRVVNEKVVGIDLGTTNSAVAAMEGGKPTIVTN 105

[Close]

BLAST of FE669306 vs. TAIR peptide
Match: AT4G24280.1 (| Symbols: cpHsc70-1 | chloroplast heat shock protein 70-1 | chr4:12590094-12593437 FORWARD LENGTH=718)

HSP 1 Score: 68.9366 bits (167), Expect = 1.347e-12
Identity = 52/103 (50.49%), Postives = 61/103 (59.22%), Query Frame = 3

Query:  192 QIH---GLGTASFSSLKKPNSTSSNS---RTLFLGHRLNSNHVSFPRAAFPKLRAKTLRKGFT-------LRVVNEKVVGIDLGTTNSAVAAMEGRKPTIITN 461
            QIH   G+G AS SS K+  +    +   R+ F G R  +   S P +AF  LR  T   G         +RVVNEKVVGIDLGTTNSAVAAMEG KPTI+TN
Sbjct:    7 QIHVLGGIGFASSSSSKRNLNGKGGTFMPRSAFFGTR--TGPFSTPTSAF--LRMGTRNGGGASRYAVGPVRVVNEKVVGIDLGTTNSAVAAMEGGKPTIVTN 105

The following BLAST results are available for this feature:

BLAST of FE669306 vs. SwissProt
BLAST of FE669306 vs. TrEMBL
BLAST of FE669306 vs. TAIR peptide

BLAST of FE669306 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
HSP7S_PEA	1.147e-35	83.33	Stromal 70 kDa heat shock-related protein, chlorop...	[more]
HSP7S_CUCMA	6.561e-7	89.29	Stromal 70 kDa heat shock-related protein, chlorop...	[more]

BLAST of FE669306 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q1SKX2_MEDTR	1.915e-28	75.53	Chaperone DnaK OS=Medicago truncatula GN=MtrDRAFT_...	[more]
Q39641_CUCSA	2.122e-19	58.06	Heat shock protein 70 OS=Cucumis sativus GN=hsp70 ...	[more]
A2TJV6_CUCSA	2.122e-19	58.06	Chloroplast HSP70 OS=Cucumis sativus PE=3 SV=1	[more]
O04056_CITLA	4.727e-19	58.06	Heat shock protein 70 OS=Citrullus lanatus PE=2 SV...	[more]
A5BEE7_VITVI	4.727e-19	63.16	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9SKC7_RICCO	1.740e-13	56.52	Heat shock protein, putative OS=Ricinus communis G...	[more]
O50036_SPIOL	1.128e-12	48.98	Heat shock 70 protein OS=Spinacia oleracea GN=HSC7...	[more]
D7MPG3_ARALY	1.473e-12	52.08	Cphsc70-2 OS=Arabidopsis lyrata subsp. lyrata GN=A...	[more]
Q9M637_ARATH	5.596e-12	49.50	Heat shock protein 70 OS=Arabidopsis thaliana GN=H...	[more]
Q9LTX9_ARATH	5.596e-12	51.49	Heat shock protein 70 OS=Arabidopsis thaliana GN=A...	[more]

BLAST of FE669306 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G49910.1	4.188e-14	51.49	\| Symbols: CPHSC70-2EAT SHOCK PROTEIN 70-2, HSC70-...	[more]
AT4G24280.1	1.347e-12	50.49	\| Symbols: cpHsc70-1 \| chloroplast heat shock prot...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 40..49 score
None	No IPR available	GENE3D	3.30.420.40	no description	coord: 127..153 score: 6.5
None	No IPR available	PANTHER	PTHR19375:SF1	HEAT SHOCK PROTEIN 70 (HSP70)	coord: 124..153 score: 7.5
None	No IPR available	SEG	seg	seg	coord: 39..63 score
None	No IPR available	SUPERFAMILY	53067	Actin-like ATPase domain	coord: 127..153 score: 2.9
None	No IPR available	SEG	seg	seg	coord: 9..27 score
IPR001023	Heat shock protein Hsp70	PANTHER	PTHR19375	HEAT SHOCK PROTEIN 70KDA	coord: 124..153 score: 7.5
IPR013126	Heat shock protein 70	PFAM	PF00012	HSP70	coord: 128..153 score: 4.6
IPR018181	Heat shock protein 70, conserved site	PROSITE	PS00297	HSP70_1	coord: 131..138 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE669306 ID=FE669306; Name=FE669306; organism=Cicer arietinum; type=EST; length=463bp
ATTCCACACGCTCGCTCGCTCACTCACTCTCTCATCTCCTCAACTTCAAC
GCTTCAACCTCATCCATTTCTCTACTTGCTACTTGTACTTTTATTCTATC
CTTCAACAACTGCAAATTAGGGTTAGGGTTAGCGTTAGCGTTAGGGTTAA
TTTCATTTCTCTCTATCTCTCTCATGGCTTCCTCTAGTGCCCAGATTCAC
GGTCTCGGAACAGCCTCTTTCTCTTCTCTCAAAAAACCCAATTCCACTTC
CTCTAATTCCCGTACCCTATTCTTAGGCCACCGTCTCAATTCAAATCACG
TCTCCTTCCCGCGCGCCGCATTTCCTAAATTGAGAGCCAAAACCCTAAGG
AAGGGTTTCACTTTGAGAGTTGTCAATGAGAAAGTTGTTGGTATCGATTT
GGGAACCACCAATTCTGCTGTCGCTGCCATGGAAGGTAGAAAACCCACCA
TCATTACCAACGC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005524	ATP binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001023	Heat shock protein Hsp70
IPR013126	Hsp_70_fam
IPR018181	Heat_shock_70_CS

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