FE672874

EST Overview


Name	FE672874
Unique Name	FE672874
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	449

Libraries


Library Name	Type
LIBEST_022820 Drought-stressed chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of FE672874 vs. SwissProt
Match: NFS1_ARATH (Cysteine desulfurase 1, mitochondrial OS=Arabidopsis thaliana GN=NIFS PE=1 SV=1)

HSP 1 Score: 254.603 bits (649), Expect = 1.394e-67
Identity = 120/144 (83.33%), Postives = 134/144 (93.06%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            ++ KKHVITTQTEHKCVLDSCRHLQQEGF+VTYLPV++DGLVDLE LR++I PDTGLVS+MAVNNEIGVVQPMEEIG ICKE NVPFHTDAAQA+ KI VDV KWNV+LMS+S HKIYGPKGVGALY++RRPRIR+EP MNGGG
Sbjct:  139 KDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKTDGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIGKIPVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGG 282

[Close]

BLAST of FE672874 vs. SwissProt
Match: NFS1_DICDI (Probable cysteine desulfurase, mitochondrial OS=Dictyostelium discoideum GN=nfs1 PE=1 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 7.174e-56
Identity = 99/144 (68.75%), Postives = 124/144 (86.11%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            +EKK H+ITT TEHKC+LDSCRHL+ EGF VTYLPV  +GLVDLE L+++ITP T LV++MAVNNEIGVVQP++EIG+IC+E  V FHTDAAQA+ KI +DV+  N+ L+S+SGHKIYGPKGVGAL+++RRPR+R+EP   GGG
Sbjct:  136 KEKKNHIITTVTEHKCILDSCRHLEMEGFKVTYLPVGENGLVDLELLKNTITPQTSLVTIMAVNNEIGVVQPIKEIGKICRENGVFFHTDAAQAVGKIPIDVNDMNIDLLSISGHKIYGPKGVGALFVRRRPRVRIEPITTGGG 279

[Close]

BLAST of FE672874 vs. SwissProt
Match: NFS1_CANMA (Cysteine desulfurase, mitochondrial OS=Candida maltosa GN=SPL1 PE=3 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 6.714e-54
Identity = 94/144 (65.28%), Postives = 123/144 (85.42%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            ++ KKH+ITTQTEHKCVLDS RH+Q EGFD+TYLPV S+GL++L+ L+ +I  DT LVSVMAVNNEIGV+QP++EIGQIC+E  + FHTDAAQA  KI +DV++ N+ L+S+S HKIYGPKG+GA Y++RRPR+R++P + GGG
Sbjct:  169 KKTKKHIITTQTEHKCVLDSARHMQDEGFDITYLPVNSEGLINLDDLKKAIRKDTVLVSVMAVNNEIGVIQPLKEIGQICRENKIFFHTDAAQAYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGG 312

[Close]

BLAST of FE672874 vs. SwissProt
Match: NFS1_DROME (Probable cysteine desulfurase, mitochondrial OS=Drosophila melanogaster GN=CG12264 PE=2 SV=1)

HSP 1 Score: 206.453 bits (524), Expect = 4.352e-53
Identity = 95/142 (66.90%), Postives = 122/142 (85.92%), Query Frame = 3

Query:   24 KKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            KK+HVITTQTEHKCVLDSCR L+ EGF VTYLPV ++GL+DL++L ++IT +T LVS+M VNNEIGV QP++EIG++C+   V FHTDAAQA+ K+ +DV+  N+ LMS+SGHKIYGPKGVGALY++RRPR+R+EP  +GGG
Sbjct:  150 KKRHVITTQTEHKCVLDSCRALENEGFKVTYLPVLANGLIDLQQLEETITSETSLVSIMTVNNEIGVRQPVDEIGKLCRSRRVFFHTDAAQAVGKVPLDVNAMNIDLMSISGHKIYGPKGVGALYVRRRPRVRLEPIQSGGG 291

[Close]

BLAST of FE672874 vs. SwissProt
Match: NFS1_CANAL (Cysteine desulfurase, mitochondrial OS=Candida albicans GN=NFS1 PE=3 SV=1)

HSP 1 Score: 206.453 bits (524), Expect = 4.352e-53
Identity = 93/144 (64.58%), Postives = 123/144 (85.42%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            ++ KKH+ITTQTEHKCVLDS RH+Q EGF+VTYLPV S+GL++L+ L+ +I  DT LVS+MAVNNEIGV+QP++EIG+IC+E  V FHTDAAQA  KI +DV++ N+ L+S+S HKIYGPKG+GA Y++RRPR+R++P + GGG
Sbjct:  173 KKTKKHIITTQTEHKCVLDSARHMQDEGFEVTYLPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQAYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVRRRPRVRLDPIITGGG 316

[Close]

BLAST of FE672874 vs. SwissProt
Match: NFS1_PONAB (Cysteine desulfurase, mitochondrial OS=Pongo abelii GN=NFS1 PE=2 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 1.654e-52
Identity = 96/144 (66.67%), Postives = 117/144 (81.25%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            R +KKH+ITTQTEHKCVLDSCR L+ EGF VTYLPV+  G++DL++L  +I PDT LVSVM VNNEIGV QP+ EIG+IC    V FHTDAAQA+ KI +DV+   + LMS+SGHKIYGPKGVGA+Y++RRPR+RVE   +GGG
Sbjct:  143 RSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQSGGG 286

[Close]

BLAST of FE672874 vs. SwissProt
Match: NFS1_HUMAN (Cysteine desulfurase, mitochondrial OS=Homo sapiens GN=NFS1 PE=1 SV=3)

HSP 1 Score: 204.527 bits (519), Expect = 1.654e-52
Identity = 96/144 (66.67%), Postives = 117/144 (81.25%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            R +KKH+ITTQTEHKCVLDSCR L+ EGF VTYLPV+  G++DL++L  +I PDT LVSVM VNNEIGV QP+ EIG+IC    V FHTDAAQA+ KI +DV+   + LMS+SGHKIYGPKGVGA+Y++RRPR+RVE   +GGG
Sbjct:  143 RSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQSGGG 286

[Close]

BLAST of FE672874 vs. SwissProt
Match: NFS1_SCHPO (Probable cysteine desulfurase, mitochondrial OS=Schizosaccharomyces pombe GN=SPBC21D10.11c PE=2 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 3.684e-52
Identity = 91/144 (63.19%), Postives = 124/144 (86.11%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            + +KKH+++ QTEHKCVLDS R LQ+EGF+VT+LPV+++GL++L++LRD+I PDT  VSVMAVNNEIGV QP+EEIG+IC++  V FH+DAAQ   KI +DV++ N+ LMS+S HKIYGPKG+GA Y++RRPR+R+EP ++GGG
Sbjct:  185 KSRKKHLVSVQTEHKCVLDSLRALQEEGFEVTFLPVQTNGLINLDELRDAIRPDTVCVSVMAVNNEIGVCQPLEEIGKICRQKKVFFHSDAAQGYGKIDIDVNRMNIDLMSISAHKIYGPKGIGAAYVRRRPRVRLEPLISGGG 328

[Close]

BLAST of FE672874 vs. SwissProt
Match: NFS1_RAT (Cysteine desulfurase, mitochondrial OS=Rattus norvegicus GN=Nfs1 PE=2 SV=1)

HSP 1 Score: 202.986 bits (515), Expect = 4.812e-52
Identity = 94/144 (65.28%), Postives = 117/144 (81.25%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            R +KKH++TTQTEHKCVLDSCR L+ EGF VTYLPV+  G++DL++L  +I PDT LVSVM VNNEIGV QP+ EIGQIC    + FHTDAAQA+ KI +DV+   + LMS+SGHK+YGPKGVGA+Y++RRPR+RVE   +GGG
Sbjct:  137 RSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGQICSSRKLYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRVEALQSGGG 280

[Close]

BLAST of FE672874 vs. SwissProt
Match: NFS1_YEAST (Cysteine desulfurase, mitochondrial OS=Saccharomyces cerevisiae GN=NFS1 PE=1 SV=2)

HSP 1 Score: 202.601 bits (514), Expect = 6.284e-52
Identity = 89/144 (61.81%), Postives = 124/144 (86.11%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            ++ KKH+ITT+TEHKCVL++ R + +EGF+VT+L V+  GL+DL++L D+I PDT LVSVMAVNNEIGV+QP++EIG IC++  + FHTDAAQA  KIH+DV++ N+ L+S+S HKIYGPKG+GA+Y++RRPR+R+EP ++GGG
Sbjct:  184 KKTKKHIITTRTEHKCVLEAARAMMKEGFEVTFLNVDDQGLIDLKELEDAIRPDTCLVSVMAVNNEIGVIQPIKEIGAICRKNKIYFHTDAAQAYGKIHIDVNEMNIDLLSISSHKIYGPKGIGAIYVRRRPRVRLEPLLSGGG 327

[Close]

BLAST of FE672874 vs. TrEMBL
Match: B9HG88_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_718085 PE=3 SV=1)

HSP 1 Score: 268.47 bits (685), Expect = 1.578e-70
Identity = 126/144 (87.50%), Postives = 138/144 (95.83%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            +EKK+HV+TTQTEHKCVLDSCRHLQQEGF+VTYLPV +DG+VDLEKL+ SI PDTGLVSVMAVNNEIGV+QPMEEIG+ICKE NVPFHTDAAQAL KI +DVDKWNVSLMSLSGHKIYGPKGVGALY++RRPRIRVEPQMNGGG
Sbjct:  138 KEKKRHVVTTQTEHKCVLDSCRHLQQEGFEVTYLPVGNDGIVDLEKLKGSIRPDTGLVSVMAVNNEIGVIQPMEEIGEICKELNVPFHTDAAQALGKIPIDVDKWNVSLMSLSGHKIYGPKGVGALYMRRRPRIRVEPQMNGGG 281

[Close]

BLAST of FE672874 vs. TrEMBL
Match: B9T178_RICCO (Cysteine desulfurylase, putative OS=Ricinus communis GN=RCOM_0338870 PE=3 SV=1)

HSP 1 Score: 264.618 bits (675), Expect = 2.279e-69
Identity = 124/144 (86.11%), Postives = 139/144 (96.53%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            +EKK+HVITTQTEHKCVLDSCR+LQQEGF+VTYLPV++DG+VDLEKLR++I  DTGLVSVMAVNNEIGV+QPMEEIG+ICKEF VPFHTDAAQAL KI +DVDKWNVSLMSLSGHKIYGPKG+GALY++RRPRIRVEPQMNGGG
Sbjct:  139 KEKKRHVITTQTEHKCVLDSCRYLQQEGFEVTYLPVKNDGIVDLEKLRNAIRADTGLVSVMAVNNEIGVIQPMEEIGKICKEFKVPFHTDAAQALGKIPIDVDKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGG 282

[Close]

BLAST of FE672874 vs. TrEMBL
Match: D7MUL5_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_919916 PE=4 SV=1)

HSP 1 Score: 254.988 bits (650), Expect = 1.806e-66
Identity = 120/144 (83.33%), Postives = 135/144 (93.75%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            R+ KKHVITTQTEHKCVLDSCRHLQQEGF+VTYLPV++DGLVDLE L+++I PDTGLVS+MAVNNEIGVVQPMEEIG+ICKE NVPFHTDAAQA+ KI VDV KWNV+LMS+S HKIYGPKGVGALY++RRPRIR+EP MNGGG
Sbjct:  139 RDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKTDGLVDLEMLKEAIRPDTGLVSIMAVNNEIGVVQPMEEIGRICKEHNVPFHTDAAQAIGKIPVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGG 282

[Close]

BLAST of FE672874 vs. TrEMBL
Match: A8MSF1_ARATH (AT5G65720 protein OS=Arabidopsis thaliana GN=AT5G65720 PE=2 SV=1)

HSP 1 Score: 254.603 bits (649), Expect = 2.358e-66
Identity = 120/144 (83.33%), Postives = 134/144 (93.06%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            ++ KKHVITTQTEHKCVLDSCRHLQQEGF+VTYLPV++DGLVDLE LR++I PDTGLVS+MAVNNEIGVVQPMEEIG ICKE NVPFHTDAAQA+ KI VDV KWNV+LMS+S HKIYGPKGVGALY++RRPRIR+EP MNGGG
Sbjct:   11 KDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKTDGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIGKIPVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGG 154

[Close]

BLAST of FE672874 vs. TrEMBL
Match: A5B9Q3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_036314 PE=3 SV=1)

HSP 1 Score: 251.906 bits (642), Expect = 1.528e-65
Identity = 119/144 (82.64%), Postives = 133/144 (92.36%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            +EKK+HVITTQTEHKCVLDSCRHLQQEGF+VTYLPV SDG+VD++ +R +I PDTGLVSVM VNNEIGV+QP+EEIG IC+E  VPFHTDAAQAL KI VDV+K NVSLMSLSGHKIYGPKGVGALY++RRPRIRVEPQMNGGG
Sbjct:  101 KEKKRHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVGSDGIVDIDLMRSAIRPDTGLVSVMTVNNEIGVIQPIEEIGNICRELGVPFHTDAAQALGKIPVDVEKMNVSLMSLSGHKIYGPKGVGALYMRRRPRIRVEPQMNGGG 244

[Close]

BLAST of FE672874 vs. TrEMBL
Match: B6T4S0_MAIZE (Cysteine desulfurase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 247.669 bits (631), Expect = 2.883e-64
Identity = 118/144 (81.94%), Postives = 132/144 (91.67%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            RE+++HVITTQTEHKCVLDSCR+LQQEGF+VTYLPV  DGLVDL +L D+I PDTGLVSVMAVNNEIGVVQP+EEIG+ICKE  VPFHTDAAQAL KI +DV++  + LMSLSGHKIYGPKGVGALYL+RRPRIRVEPQMNGGG
Sbjct:  137 RERRRHVITTQTEHKCVLDSCRYLQQEGFEVTYLPVRKDGLVDLAQLEDAIRPDTGLVSVMAVNNEIGVVQPLEEIGRICKEKGVPFHTDAAQALGKIPIDVNRMGIGLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGG 280

[Close]

BLAST of FE672874 vs. TrEMBL
Match: B4FLP7_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 247.669 bits (631), Expect = 2.883e-64
Identity = 118/144 (81.94%), Postives = 132/144 (91.67%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            RE+++HVITTQTEHKCVLDSCR+LQQEGF+VTYLPV  DGLVDL +L D+I PDTGLVSVMAVNNEIGVVQP+EEIG+ICKE  VPFHTDAAQAL KI +DV++  + LMSLSGHKIYGPKGVGALYL+RRPRIRVEPQMNGGG
Sbjct:  137 RERRRHVITTQTEHKCVLDSCRYLQQEGFEVTYLPVRKDGLVDLAQLEDAIRPDTGLVSVMAVNNEIGVVQPLEEIGRICKEKGVPFHTDAAQALGKIPIDVNRMGIGLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGG 280

[Close]

BLAST of FE672874 vs. TrEMBL
Match: B6T3M2_MAIZE (Cysteine desulfurase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 246.899 bits (629), Expect = 4.917e-64
Identity = 116/144 (80.56%), Postives = 132/144 (91.67%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            RE+++HVITTQTEHKCVLDSCR+LQQEGF++TYLPV  DGLVDL +L D+I PDTGLVS+MAVNNEIGVVQP+EEIG+ICKE  VPFHTDAAQAL KI +DV++  + LMSLSGHKIYGPKGVGALYL+RRPRIRVEPQMNGGG
Sbjct:  137 RERRRHVITTQTEHKCVLDSCRYLQQEGFEITYLPVRKDGLVDLAQLEDAIRPDTGLVSIMAVNNEIGVVQPLEEIGRICKEKGVPFHTDAAQALGKIPIDVNRMGIGLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGG 280

[Close]

BLAST of FE672874 vs. TrEMBL
Match: Q6ERL4_ORYSJ (Os09g0338400 protein OS=Oryza sativa subsp. japonica GN=OJ1118_A10.25 PE=2 SV=1)

HSP 1 Score: 239.58 bits (610), Expect = 7.850e-62
Identity = 113/144 (78.47%), Postives = 131/144 (90.97%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            R++++HV+TTQTEHKCVLDSCR+LQQEGF+VTYLPV  DGLVD+ +L D+I PDTGLVSVMAVNNEIGVVQP+EEIG+ICKE  VPFHTDAAQAL KI +DV++  + LMSLS HKIYGPKGVGALYL+RRPRIRVEPQM+GGG
Sbjct:  144 RDRRRHVVTTQTEHKCVLDSCRYLQQEGFEVTYLPVRPDGLVDVAQLADAIRPDTGLVSVMAVNNEIGVVQPLEEIGRICKEKGVPFHTDAAQALGKIPIDVNQMGIGLMSLSAHKIYGPKGVGALYLRRRPRIRVEPQMSGGG 287

[Close]

BLAST of FE672874 vs. TrEMBL
Match: D5AA74_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 231.876 bits (590), Expect = 1.637e-59
Identity = 106/144 (73.61%), Postives = 130/144 (90.28%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            ++KK+HVITTQTEHKCVLDSCRHLQQEGF+VTYLPV+ DGL+D+++L+ +I PDTGLVSVM V+NEIGV+QP+EEIG+IC++  +  HTDAAQA  KI V+VD+  V LMSLSGHKIYGPKG+GALY++RRPR+RVEPQMNGGG
Sbjct:  141 KDKKRHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKKDGLIDIDELKAAIRPDTGLVSVMTVHNEIGVIQPIEEIGEICRQNKIFLHTDAAQAAGKIPVNVDEMKVDLMSLSGHKIYGPKGIGALYMRRRPRVRVEPQMNGGG 284

[Close]

BLAST of FE672874 vs. TAIR peptide
Match: AT5G65720.2 (| Symbols: ATNIFS1, NIFS1, NFS1, ATNFS1 | nitrogen fixation S (NIFS)-like 1 | chr5:26296733-26297710 FORWARD LENGTH=325)

HSP 1 Score: 254.603 bits (649), Expect = 1.651e-68
Identity = 120/144 (83.33%), Postives = 134/144 (93.06%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            ++ KKHVITTQTEHKCVLDSCRHLQQEGF+VTYLPV++DGLVDLE LR++I PDTGLVS+MAVNNEIGVVQPMEEIG ICKE NVPFHTDAAQA+ KI VDV KWNV+LMS+S HKIYGPKGVGALY++RRPRIR+EP MNGGG
Sbjct:   11 KDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKTDGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIGKIPVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGG 154

[Close]

BLAST of FE672874 vs. TAIR peptide
Match: AT5G65720.1 (| Symbols: ATNIFS1, NIFS1, NFS1, ATNFS1 | nitrogen fixation S (NIFS)-like 1 | chr5:26296349-26297710 FORWARD LENGTH=453)

HSP 1 Score: 254.603 bits (649), Expect = 1.651e-68
Identity = 120/144 (83.33%), Postives = 134/144 (93.06%), Query Frame = 3

Query:   18 REKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGGG 449
            ++ KKHVITTQTEHKCVLDSCRHLQQEGF+VTYLPV++DGLVDLE LR++I PDTGLVS+MAVNNEIGVVQPMEEIG ICKE NVPFHTDAAQA+ KI VDV KWNV+LMS+S HKIYGPKGVGALY++RRPRIR+EP MNGGG
Sbjct:  139 KDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKTDGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIGKIPVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGG 282

[Close]

BLAST of FE672874 vs. TAIR peptide
Match: AT1G08490.1 (| Symbols: ATSUFS, SUFS, ATCPNIFS, ATNFS2, CPNIFS | chloroplastic NIFS-like cysteine desulfurase | chr1:2685980-2688547 REVERSE LENGTH=463)

HSP 1 Score: 72.7886 bits (177), Expect = 8.898e-14
Identity = 43/137 (31.39%), Postives = 69/137 (50.36%), Query Frame = 3

Query:   36 VITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRDSITPDTGLVSVMAVNNEIGVVQPMEEIGQICKEFNVPFHTDAAQALWKIHVDVDKWNVSLMSLSGHKIYGPKGVGALYLKRRPRIRVEPQMNGG 446
            ++T    H C++      Q+ G  + ++ +  D + D+ KLR+ I+P T LV+V  V+N +    P+EEI     +       DA Q++  + VDV K N   +  S HK+ GP G+G LY K      + P + GG
Sbjct:  166 ILTVAEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELISPKTKLVAVHHVSNVLASSLPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLVASSHKMCGPTGIGFLYGKSDLLHSMPPFLGGG 302

The following BLAST results are available for this feature:

BLAST of FE672874 vs. SwissProt
BLAST of FE672874 vs. TrEMBL
BLAST of FE672874 vs. TAIR peptide

BLAST of FE672874 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
NFS1_ARATH	1.394e-67	83.33	Cysteine desulfurase 1, mitochondrial OS=Arabidops...	[more]
NFS1_DICDI	7.174e-56	68.75	Probable cysteine desulfurase, mitochondrial OS=Di...	[more]
NFS1_CANMA	6.714e-54	65.28	Cysteine desulfurase, mitochondrial OS=Candida mal...	[more]
NFS1_DROME	4.352e-53	66.90	Probable cysteine desulfurase, mitochondrial OS=Dr...	[more]
NFS1_CANAL	4.352e-53	64.58	Cysteine desulfurase, mitochondrial OS=Candida alb...	[more]
NFS1_PONAB	1.654e-52	66.67	Cysteine desulfurase, mitochondrial OS=Pongo abeli...	[more]
NFS1_HUMAN	1.654e-52	66.67	Cysteine desulfurase, mitochondrial OS=Homo sapien...	[more]
NFS1_SCHPO	3.684e-52	63.19	Probable cysteine desulfurase, mitochondrial OS=Sc...	[more]
NFS1_RAT	4.812e-52	65.28	Cysteine desulfurase, mitochondrial OS=Rattus norv...	[more]
NFS1_YEAST	6.284e-52	61.81	Cysteine desulfurase, mitochondrial OS=Saccharomyc...	[more]

BLAST of FE672874 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9HG88_POPTR	1.578e-70	87.50	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9T178_RICCO	2.279e-69	86.11	Cysteine desulfurylase, putative OS=Ricinus commun...	[more]
D7MUL5_ARALY	1.806e-66	83.33	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
A8MSF1_ARATH	2.358e-66	83.33	AT5G65720 protein OS=Arabidopsis thaliana GN=AT5G6...	[more]
A5B9Q3_VITVI	1.528e-65	82.64	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B6T4S0_MAIZE	2.883e-64	81.94	Cysteine desulfurase OS=Zea mays PE=2 SV=1	[more]
B4FLP7_MAIZE	2.883e-64	81.94	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B6T3M2_MAIZE	4.917e-64	80.56	Cysteine desulfurase OS=Zea mays PE=2 SV=1	[more]
Q6ERL4_ORYSJ	7.850e-62	78.47	Os09g0338400 protein OS=Oryza sativa subsp. japoni...	[more]
D5AA74_PICSI	1.637e-59	73.61	Putative uncharacterized protein OS=Picea sitchens...	[more]

BLAST of FE672874 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT5G65720.2	1.651e-68	83.33	\| Symbols: ATNIFS1, NIFS1, NFS1, ATNFS1 \| nitrogen...	[more]
AT5G65720.1	1.651e-68	83.33	\| Symbols: ATNIFS1, NIFS1, NFS1, ATNFS1 \| nitrogen...	[more]
AT1G08490.1	8.898e-14	31.39	\| Symbols: ATSUFS, SUFS, ATCPNIFS, ATNFS2, CPNIFS ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 21..35 score: NAcoord: 39..54 score
None	No IPR available	PANTHER	PTHR11601	CYSTEINE DESULFURYLASE	coord: 3..149 score: 4.5
IPR000192	Aminotransferase, class V/Cysteine desulfurase	PFAM	PF00266	Aminotran_5	coord: 7..149 score: 8.8
IPR015421	Pyridoxal phosphate-dependent transferase, major region, subdomain 1	GENE3D	3.40.640.10	no description	coord: 6..149 score: 3
IPR015424	Pyridoxal phosphate-dependent transferase, major domain	SUPERFAMILY	53383	PLP-dependent transferases	coord: 7..149 score: 6.2

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE672874 ID=FE672874; Name=FE672874; organism=Cicer arietinum; type=EST; length=449bp
TGTTATGCATTTCTACAAGGGAGAAGAAAAAACACGTGATAACCACGCAA
ACGGAACATAAGTGTGTTCTTGATTCTTGTCGTCATCTTCAACAGGAGGG
TTTTGATGTTACTTATCTTCCTGTTGAGTCTGATGGGTTGGTTGATTTGG
AGAAGCTTAGGGATTCTATTACGCCCGATACTGGTCTTGTTTCTGTTATG
GCTGTGAATAATGAGATTGGTGTTGTTCAACCTATGGAGGAAATTGGACA
GATTTGTAAGGAGTTTAATGTTCCTTTTCATACTGATGCAGCTCAAGCTT
TATGGAAGATTCATGTTGATGTTGATAAGTGGAACGTGAGTTTGATGTCG
TTGAGTGGTCATAAGATTTATGGGCCAAAAGGGGTTGGAGCTTTGTATCT
TAAGAGGAGACCTAGGATTCGTGTCGAGCCTCAGATGAATGGTGGTGGA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0030170	pyridoxal phosphate binding

Vocabulary: **INTERPRO**
Term	Definition
IPR000192	Aminotrans_V_dom
IPR015421	PyrdxlP-dep_Trfase_major
IPR015424	PyrdxlP-dep_Trfase

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