FE673529

EST Overview


Name	FE673529
Unique Name	FE673529
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	552

Libraries


Library Name	Type
LIBEST_022820 Drought-stressed chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of FE673529 vs. SwissProt
Match: RPA1_SCHPO (DNA-directed RNA polymerase I subunit rpa1 OS=Schizosaccharomyces pombe GN=rpa1 PE=1 SV=2)

HSP 1 Score: 125.176 bits (313), Expect = 2.130e-28
Identity = 60/131 (45.80%), Postives = 89/131 (67.94%), Query Frame = 2

Query:  158 PSSVKEKIPALQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            P S  + +P + T G++ K+ WE  +++++  +Y+N++ AIL  YGVEAA+  I+ EV +VF  YGI+V+ RHL LIAD+MT  G Y+  +R G I  +TSPF K+ FET  +F+ EAA  G VD+L +PS
Sbjct: 1532 PDSALDTVPKVITEGVNLKAIWEFYNEISMNDIYTNDIAAILRIYGVEAARNAIVHEVSSVFGVYGIAVDPRHLSLIADYMTFEGGYKAFNRMG-IEYNTSPFAKMSFETTCHFLTEAALRGDVDDLSNPS 1661

[Close]

BLAST of FE673529 vs. SwissProt
Match: RPA1_DROME (DNA-directed RNA polymerase I subunit RPA1 OS=Drosophila melanogaster GN=RpI1 PE=1 SV=2)

HSP 1 Score: 117.857 bits (294), Expect = 3.400e-26
Identity = 62/145 (42.76%), Postives = 91/145 (62.76%), Query Frame = 2

Query:  116 VIYYGEDDSKREEIPSSVKEKIPALQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            +IY G DD +              L+T GI+    ++    L++  +YSN++HAI  TYG+EAA + I++EV NVFK YGI+V+ RHL LIAD+MT  G ++P+SRKG +  S+SP  ++ FE++  F+  AA  G+ D L SPS
Sbjct: 1489 IIYKGNDDDQ-------------LLKTDGINIGEMFQHNKILDLNRLYSNDIHAIARTYGIEAASQVIVKEVSNVFKVYGITVDRRHLSLIADYMTFDGTFQPLSRKG-MEHSSSPLQQMSFESSLQFLKSAAGFGRADELSSPS 1619

[Close]

BLAST of FE673529 vs. SwissProt
Match: RPA1_DICDI (DNA-directed RNA polymerase I subunit rpa1 OS=Dictyostelium discoideum GN=polr1a PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 6.412e-25
Identity = 52/122 (42.62%), Postives = 85/122 (69.67%), Query Frame = 2

Query:  185 ALQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            ++Q+ G++    +E++D L +  +Y+N+++AIL  YGVEA ++ +  E+ NVF +YGISV+ RHL+L+ D+MT  G YR ++R G I ++TSPF K+ FET  +F+ +A+  G  D + SPS
Sbjct: 1472 SIQSEGVNLSEIFELRDKLKIDEIYTNDIYAILQKYGVEACRQAVTSEISNVFAAYGISVDKRHLILLGDYMTFEGGYRALNRIG-IENNTSPFQKMSFETTFSFLSKASLMGDYDTVTSPS 1592

[Close]

BLAST of FE673529 vs. SwissProt
Match: RPA1_YEAST (DNA-directed RNA polymerase I subunit RPA1 OS=Saccharomyces cerevisiae GN=RPA1 PE=1 SV=2)

HSP 1 Score: 112.079 bits (279), Expect = 1.866e-24
Identity = 55/121 (45.45%), Postives = 80/121 (66.12%), Query Frame = 2

Query:  188 LQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            L T G++F++ W+ +  ++V  + SN+V A+L TYGVEAA+ TI+ E+ NVF  Y ISV+ RHL LIAD MT  G Y   +R+G +  STS F+K+ +ET   F+ +A    + + LDSPS
Sbjct: 1519 LVTEGVNFQAMWDQEAFIDVDGITSNDVAAVLKTYGVEAARNTIVNEINNVFSRYAISVSFRHLDLIADMMTRQGTYLAFNRQG-METSTSSFMKMSYETTCQFLTKAVLDNEREQLDSPS 1638

[Close]

BLAST of FE673529 vs. SwissProt
Match: RPA1_MOUSE (DNA-directed RNA polymerase I subunit RPA1 OS=Mus musculus GN=Polr1a PE=1 SV=2)

HSP 1 Score: 109.768 bits (273), Expect = 9.259e-24
Identity = 56/133 (42.11%), Postives = 86/133 (64.66%), Query Frame = 2

Query:  152 EIPSSVKEKIPALQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            E  +S  EK   L T GI+    ++  + L++R +YSN++HA+ +TYG+EAA   I +E+++VF  YGI+V+ RHL L+AD+M   G Y+P++R G I  S+SP  ++ FET+  F+ +A   G  D L SPS
Sbjct: 1563 ETTNSKNEKELVLNTEGINLPELFKYSEILDLRRLYSNDIHAMANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFG-IQSSSSPLQQMTFETSFQFLKQATMMGSHDELKSPS 1694

[Close]

BLAST of FE673529 vs. SwissProt
Match: RPA1_RAT (DNA-directed RNA polymerase I subunit RPA1 OS=Rattus norvegicus GN=Polr1a PE=1 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.209e-23
Identity = 56/133 (42.11%), Postives = 86/133 (64.66%), Query Frame = 2

Query:  152 EIPSSVKEKIPALQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            E  +S  EK   L T GI+    ++  + L++R +YSN++HA+ +TYG+EAA   I +E+++VF  YGI+V+ RHL L+AD+M   G Y+P++R G I  S+SP  ++ FET+  F+ +A   G  D L SPS
Sbjct: 1562 ETINSKNEKEFVLNTEGINLPELFKYSEVLDLRRLYSNDIHAVANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFG-IQSSSSPLQQMTFETSFQFLKQATMMGSHDELKSPS 1693

[Close]

BLAST of FE673529 vs. SwissProt
Match: RPA1_HUMAN (DNA-directed RNA polymerase I subunit RPA1 OS=Homo sapiens GN=POLR1A PE=1 SV=2)

HSP 1 Score: 107.457 bits (267), Expect = 4.595e-23
Identity = 55/133 (41.35%), Postives = 86/133 (64.66%), Query Frame = 2

Query:  152 EIPSSVKEKIPALQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            E  ++  EK   L T GI+    ++  + L++R +YSN++HAI +TYG+EAA   I +E+++VF  YGI+V+ RHL L+AD+M   G Y+P++R G I  ++SP  ++ FET+  F+ +A   G  D L SPS
Sbjct: 1566 ETTNNKNEKELVLNTEGINLPELFKYAEVLDLRRLYSNDIHAIANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFG-IRSNSSPLQQMTFETSFQFLKQATMLGSHDELRSPS 1697

[Close]

BLAST of FE673529 vs. SwissProt
Match: RPOA2_PYRAB (DNA-directed RNA polymerase subunit A'' OS=Pyrococcus abyssi GN=rpoA2 PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.096e-16
Identity = 43/117 (36.75%), Postives = 65/117 (55.56%), Query Frame = 2

Query:  188 LQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNL 538
            + T G +FK   ++   ++     +NN+H I    G+EAA+  II E+ N  +  G+ V+IRH+ML+AD MT  G  RP+ R G + +  S   +  FE     + EAA  G+VDNL
Sbjct:  245 IYTEGSNFKQVLKVPG-VDPTRTKTNNIHEIAEVLGIEAARNAIIEEIMNTMREQGLEVDIRHIMLVADIMTLDGVVRPIGRHGVVGEKASVLARAAFEITVQHLFEAAERGEVDNL 360

[Close]

BLAST of FE673529 vs. SwissProt
Match: RPOA2_HALMO (DNA-directed RNA polymerase subunit A'' OS=Halococcus morrhuae GN=rpoA2 PE=1 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 4.166e-16
Identity = 42/94 (44.68%), Postives = 56/94 (59.57%), Query Frame = 2

Query:  263 NNVHAILSTYGVEAAKETIIREVQNVFKSYGIS-VNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLD 541
            NN+H +    GVEAA+ETII E  N  +  G+  VNIRHLML+AD MT  G    + R G   +  S   +  FE   N +++AA HG+VD+LD
Sbjct:  265 NNIHEVYRNLGVEAARETIIDETMNTLEEQGLGDVNIRHLMLVADIMTAEGTIESIGRHGISGNKNSVLARAAFEVTVNHLLDAAVHGEVDDLD 358

[Close]

BLAST of FE673529 vs. SwissProt
Match: RPOA2_PYRHO (DNA-directed RNA polymerase subunit A'' OS=Pyrococcus horikoshii GN=rpoA2 PE=3 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.441e-16
Identity = 43/117 (36.75%), Postives = 64/117 (54.70%), Query Frame = 2

Query:  188 LQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNL 538
            + T G +FK   ++   ++     +NN+H I    G+EAA+  II E+ N     G+ V+IRH+ML+AD MT  G  RP+ R G + +  S   +  FE     + EAA  G+VDNL
Sbjct:  245 IYTEGSNFKQVLKVPG-VDPTRTRTNNIHEIAEVLGIEAARNAIIEEIINTMHEQGLEVDIRHIMLVADIMTLDGVVRPIGRHGVVGEKASVLARAAFEITVQHLFEAAERGEVDNL 360

[Close]

BLAST of FE673529 vs. TrEMBL
Match: B9SL65_RICCO (DNA-directed RNA polymerase OS=Ricinus communis GN=RCOM_0090500 PE=3 SV=1)

HSP 1 Score: 246.899 bits (629), Expect = 6.543e-64
Identity = 117/179 (65.36%), Postives = 147/179 (82.12%), Query Frame = 2

Query:   14 IPIPQIAQKTAEKVCIQNFGKVGQCQAITCKESGVIYYGEDDSKREEIPSSVKEKIPALQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            I + +IAQKTA+KV IQN GK+ QC+   CKES VIYYG+D  +R ++   VKEK+PAL  +G+ F +FW+MQD L+VRY+YSNN+HA+L TYGVEAA+ETIIRE+ +VFKSYGI+V+ RHL LIADFMTH+G YRPMSR G IA+S SPF K+ FETAS FIVEAA HG++DNL++PS
Sbjct: 1484 ILLAEIAQKTAKKVYIQNPGKIEQCRVTDCKESQVIYYGKDPKERVDLKPDVKEKVPALHATGVDFNTFWKMQDHLDVRYIYSNNIHAMLKTYGVEAARETIIREINHVFKSYGIAVSNRHLSLIADFMTHTGGYRPMSRMGGIAESISPFSKMSFETASKFIVEAALHGEIDNLETPS 1662

[Close]

BLAST of FE673529 vs. TrEMBL
Match: D7TI03_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00033327001 PE=4 SV=1)

HSP 1 Score: 241.506 bits (615), Expect = 2.749e-62
Identity = 117/179 (65.36%), Postives = 141/179 (78.77%), Query Frame = 2

Query:   14 IPIPQIAQKTAEKVCIQNFGKVGQCQAITCKESGVIYYGEDDSKREEIPSSVKEKIPALQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            I + QIAQK A KV I++ GK+  CQ I C +  VIYYG D  KRE IP   K+K+PALQT+G+ F +FW+MQD+L+VRYVYSNNVHA+L+T+GVEAA+ TII+EV NVF +YG+ VNIRHL LIADFMTHSG YRPM+R G IA+S SPF K+ FETAS FIVEAA HG  DNL+S S
Sbjct: 1467 ILLAQIAQKAANKVYIRSSGKIDLCQVIDCNKDQVIYYGRDPKKRENIPGEEKKKLPALQTAGVDFTAFWKMQDELDVRYVYSNNVHAMLNTFGVEAARATIIKEVFNVFNAYGVKVNIRHLSLIADFMTHSGGYRPMNRHGGIAESVSPFSKMTFETASKFIVEAASHGMTDNLESAS 1645

[Close]

BLAST of FE673529 vs. TrEMBL
Match: B9HA71_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_762153 PE=4 SV=1)

HSP 1 Score: 239.58 bits (610), Expect = 1.045e-61
Identity = 115/179 (64.25%), Postives = 144/179 (80.45%), Query Frame = 2

Query:   14 IPIPQIAQKTAEKVCIQNFGKVGQCQAITCKESGVIYYGEDDSKREEIPSSVKEKIPALQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            I + +IAQKTA+KVCIQN GKV +CQ   CKE+ VIYYG+D  +R +I    K+KIPAL T G+ F +FW+MQD L+VRY+YSN++H +L  YGVEAA+ETIIRE+++VF SYGISVN RHL LIAD+MTH+G YRPMSR G I++S SP  K+ FETAS FIVEAA HG+VDNL++PS
Sbjct:  262 ILLAEIAQKTAKKVCIQNPGKVQRCQVTDCKENQVIYYGKDPKRRIDIEPGEKQKIPALHTIGVDFNTFWKMQDHLDVRYMYSNSIHGMLKAYGVEAARETIIREIKHVFNSYGISVNTRHLSLIADYMTHTGEYRPMSRIGGISESISPLSKMSFETASKFIVEAALHGEVDNLEAPS 440

[Close]

BLAST of FE673529 vs. TrEMBL
Match: Q9SVY0_ARATH (DNA-directed RNA polymerase OS=Arabidopsis thaliana GN=F15B8.150 PE=3 SV=1)

HSP 1 Score: 214.157 bits (544), Expect = 4.700e-54
Identity = 103/179 (57.54%), Postives = 138/179 (77.09%), Query Frame = 2

Query:   14 IPIPQIAQKTAEKVCIQNFGKVGQCQAITCKESGVIYYGEDDSKREEIPSSVKEKIPALQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            I + QIAQ+TA+KV IQN GK+ +C    C +  VIY+G++  +R EI +  K+  PAL  SG+ F + WE QD L+VRY+YSN++H +L+ +GVEAA+ETIIRE+ +VFKSYGISV+IRHL LIAD+MT SG YRPMSR G IA+STSPF ++ FETA+ FIV+AA +G+ D L++PS
Sbjct: 1468 ILLAQIAQQTAQKVYIQNSGKIERCTVANCGDPQVIYHGDNPKERREISNDEKKASPALHASGVDFPALWEFQDKLDVRYLYSNSIHDMLNIFGVEAARETIIREINHVFKSYGISVSIRHLNLIADYMTFSGGYRPMSRMGGIAESTSPFCRMTFETATKFIVQAATYGEKDTLETPS 1646

[Close]

BLAST of FE673529 vs. TrEMBL
Match: D7LVV3_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_486216 PE=4 SV=1)

HSP 1 Score: 214.157 bits (544), Expect = 4.700e-54
Identity = 103/179 (57.54%), Postives = 138/179 (77.09%), Query Frame = 2

Query:   14 IPIPQIAQKTAEKVCIQNFGKVGQCQAITCKESGVIYYGEDDSKREEIPSSVKEKIPALQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            I + QIAQKTA+KV IQ+ GK+ +C    C +  VIY+G++  +R EI +  K+  PAL  SG+ F + WE QD L+VRY+YSN++H +L+ +GVEAA+ETIIRE+ +VFKSYGISV+IRHL LIAD+MT SG YRPMSR G IA+STSPF ++ FETA+ FIV+AA +G+ D L++PS
Sbjct: 1476 ILLAQIAQKTAQKVYIQDSGKIERCTVANCGDPQVIYHGDNPKERREISNDEKKASPALHASGVDFPALWEFQDKLDVRYLYSNSIHDMLNVFGVEAARETIIREINHVFKSYGISVSIRHLNLIADYMTFSGGYRPMSRMGGIAESTSPFCRMTFETATKFIVQAATYGEKDTLETPS 1654

[Close]

BLAST of FE673529 vs. TrEMBL
Match: Q69X40_ORYSJ (DNA-directed RNA polymerase OS=Oryza sativa subsp. japonica GN=P0429G06.12 PE=3 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 4.886e-35
Identity = 84/179 (46.93%), Postives = 116/179 (64.80%), Query Frame = 2

Query:   14 IPIPQIAQKTAEKVCIQNFGKVGQCQAITCKESGVIYYGEDDSKREEIPSSVKEKIPALQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            I + QIAQ+TA  V ++    + +C+                +K ++I ++       LQT+G++F+ F ++ D L++  V SN++HA+L+TYGVEAA+ TII EV+ VF +YGI V++RHL LIADFMT  G YRPMSR G    STSPF K+ FETA+ FIVEAA HG+ D LD PS
Sbjct: 1555 ILLAQIAQRTARSVFVKACKNIDRCEV---------------NKPKKIDNNTINTPITLQTAGVNFEVFHKLVDYLDINEVRSNDIHAMLNTYGVEAARATIIGEVKGVFGAYGIHVDMRHLNLIADFMTFDGGYRPMSRLGMGQFSTSPFGKMTFETATKFIVEAASHGESDTLDGPS 1718

[Close]

BLAST of FE673529 vs. TrEMBL
Match: Q0DAZ6_ORYSJ (Os06g0612200 protein OS=Oryza sativa subsp. japonica GN=Os06g0612200 PE=4 SV=2)

HSP 1 Score: 150.984 bits (380), Expect = 4.886e-35
Identity = 84/179 (46.93%), Postives = 116/179 (64.80%), Query Frame = 2

Query:   14 IPIPQIAQKTAEKVCIQNFGKVGQCQAITCKESGVIYYGEDDSKREEIPSSVKEKIPALQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            I + QIAQ+TA  V ++    + +C+                +K ++I ++       LQT+G++F+ F ++ D L++  V SN++HA+L+TYGVEAA+ TII EV+ VF +YGI V++RHL LIADFMT  G YRPMSR G    STSPF K+ FETA+ FIVEAA HG+ D LD PS
Sbjct:   44 ILLAQIAQRTARSVFVKACKNIDRCEV---------------NKPKKIDNNTINTPITLQTAGVNFEVFHKLVDYLDINEVRSNDIHAMLNTYGVEAARATIIGEVKGVFGAYGIHVDMRHLNLIADFMTFDGGYRPMSRLGMGQFSTSPFGKMTFETATKFIVEAASHGESDTLDGPS 207

[Close]

BLAST of FE673529 vs. TrEMBL
Match: C0JAC0_ORYCO (DNA-directed RNA polymerase OS=Oryza coarctata PE=3 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 5.403e-34
Identity = 71/121 (58.68%), Postives = 93/121 (76.86%), Query Frame = 2

Query:  188 LQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            LQT+G++F+ FW + D L++  + SN++HA+L  YGVEAA+ TII+EV  VF +YGI+V++RHL +IADFMT  G YRPMSR+G    STSPF K+ FETA+ FIVEAA HG+ D LD PS
Sbjct: 1680 LQTAGVNFEVFWNLVDYLDINEIRSNDIHAMLKIYGVEAARATIIQEVSGVFSAYGINVDMRHLNMIADFMTFDGGYRPMSRQGMGQFSTSPFGKMTFETATKFIVEAASHGESDTLDGPS 1800

[Close]

BLAST of FE673529 vs. TrEMBL
Match: C5YUX4_SORBI (Putative uncharacterized protein Sb09g027230 OS=Sorghum bicolor GN=Sb09g027230 PE=4 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 7.801e-33
Identity = 79/179 (44.13%), Postives = 109/179 (60.89%), Query Frame = 2

Query:   14 IPIPQIAQKTAEKVCIQNFGKVGQCQAITCKESGVIYYGEDDSKREEIPSSVKEKIPALQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            I + QIA+K A  + ++    +  C+ +  +         DD  R             +QT+G++F+  W + + ++V  + SN++HAIL TYGVEAA+ TII EV  VFK YGI V++RHL +IADFMT  G YRPM+R G    STSPF K+ FETA+ FIVEAA HG+ D L+ PS
Sbjct:  125 ILLAQIAKKIARTIFVKACNNIDDCKIVKPR---------DDHAR-------------IQTAGVNFEVVWNLNEYISVHDITSNDIHAILKTYGVEAARNTIINEVSGVFKPYGIDVDMRHLSMIADFMTLDGGYRPMNRIGIGQFSTSPFGKMTFETATKFIVEAATHGEADFLECPS 281

[Close]

BLAST of FE673529 vs. TrEMBL
Match: B9FU47_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_21960 PE=4 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 1.127e-31
Identity = 71/121 (58.68%), Postives = 92/121 (76.03%), Query Frame = 2

Query:  188 LQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            ++ SG++F+ F ++ D L++  V SN++HA+L+TYGVEAA+ TII EV+ VF +YGI V++RHL LIADFMT  G YRPMSR G    STSPF K+ FETA+ FIVEAA HG+ D LD PS
Sbjct:   59 VKASGVNFEVFHKLVDYLDINEVRSNDIHAMLNTYGVEAARATIIGEVKGVFGAYGIHVDMRHLNLIADFMTFDGGYRPMSRLGMGQFSTSPFGKMTFETATKFIVEAASHGESDTLDGPS 179

[Close]

BLAST of FE673529 vs. TAIR peptide
Match: AT3G57660.1 (| Symbols: NRPA1 | nuclear RNA polymerase A1 | chr3:21353746-21362814 FORWARD LENGTH=1670)

HSP 1 Score: 214.157 bits (544), Expect = 3.759e-56
Identity = 103/179 (57.54%), Postives = 138/179 (77.09%), Query Frame = 2

Query:   14 IPIPQIAQKTAEKVCIQNFGKVGQCQAITCKESGVIYYGEDDSKREEIPSSVKEKIPALQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLDSPS 550
            I + QIAQ+TA+KV IQN GK+ +C    C +  VIY+G++  +R EI +  K+  PAL  SG+ F + WE QD L+VRY+YSN++H +L+ +GVEAA+ETIIRE+ +VFKSYGISV+IRHL LIAD+MT SG YRPMSR G IA+STSPF ++ FETA+ FIV+AA +G+ D L++PS
Sbjct: 1468 ILLAQIAQQTAQKVYIQNSGKIERCTVANCGDPQVIYHGDNPKERREISNDEKKASPALHASGVDFPALWEFQDKLDVRYLYSNSIHDMLNIFGVEAARETIIREINHVFKSYGISVSIRHLNLIADYMTFSGGYRPMSRMGGIAESTSPFCRMTFETATKFIVQAATYGEKDTLETPS 1646

[Close]

BLAST of FE673529 vs. TAIR peptide
Match: AT5G60040.1 (| Symbols: NRPC1 | nuclear RNA polymerase C1 | chr5:24173590-24183269 FORWARD LENGTH=1376)

HSP 1 Score: 75.485 bits (184), Expect = 2.087e-14
Identity = 46/141 (32.62%), Postives = 73/141 (51.77%), Query Frame = 2

Query:  119 IYYGEDDSKREEIPSSVKEKIPALQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLD 541
            +   ED  K ++I    K K+    T+ +           +N R   SNNV  +  T G+EAA+ TII E+  V  ++G+S++IRH+ML+AD MT+ G    + R G      S  ++  FE   + +  AA  G+VDN++
Sbjct: 1200 VVVAEDMDKSKQIDGKTKWKLFVEGTNLLAVMG----TPGINGRTTTSNNVVEVSKTLGIEAARTTIIDEIGTVMGNHGMSIDIRHMMLLADVMTYRGEVLGIQRTGIQKMDKSVLMQASFERTGDHLFSAAASGKVDNIE 1336

[Close]

BLAST of FE673529 vs. TAIR peptide
Match: AT5G60040.2 (| Symbols: NRPC1 | nuclear RNA polymerase C1 | chr5:24173590-24183269 FORWARD LENGTH=1391)

HSP 1 Score: 75.0998 bits (183), Expect = 2.725e-14
Identity = 39/101 (38.61%), Postives = 60/101 (59.41%), Query Frame = 2

Query:  239 LNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNLD 541
            +N R   SNNV  +  T G+EAA+ TII E+  V  ++G+S++IRH+ML+AD MT+ G    + R G      S  ++  FE   + +  AA  G+VDN++
Sbjct: 1251 INGRTTTSNNVVEVSKTLGIEAARTTIIDEIGTVMGNHGMSIDIRHMMLLADVMTYRGEVLGIQRTGIQKMDKSVLMQASFERTGDHLFSAAASGKVDNIE 1351

[Close]

BLAST of FE673529 vs. TAIR peptide
Match: AT4G35800.1 (| Symbols: NRPB1, RPB1, RNA_POL_II_LSRNA_POL_II_LS, RNA_POL_II_LS | RNA polymerase II large subunit | chr4:16961115-16967892 REVERSE LENGTH=1839)

HSP 1 Score: 61.2326 bits (147), Expect = 4.072e-10
Identity = 32/117 (27.35%), Postives = 62/117 (52.99%), Query Frame = 2

Query:  188 LQTSGIHFKSFWEMQDDLNVRYVYSNNVHAILSTYGVEAAKETIIREVQNVFKSYGISVNIRHLMLIADFMTHSGCYRPMSRKGSIADSTSPFLKICFETASNFIVEAACHGQVDNL 538
            L T G++  +     +D++ +   SN++  I+   G+EA +  ++ E++ V    G  VN RHL ++ D MT+ G    ++R G   + T P ++  FE   + +++AA + + D L
Sbjct: 1327 LDTEGVNLLAVM-CHEDVDPKRTTSNHLIEIIEVLGIEAVRRALLDELRVVISFDGSYVNYRHLAILCDTMTYRGHLMAITRHGINRNDTGPLMRCSFEETVDILLDAAAYAETDCL 1442

The following BLAST results are available for this feature:

BLAST of FE673529 vs. SwissProt
BLAST of FE673529 vs. TrEMBL
BLAST of FE673529 vs. TAIR peptide

BLAST of FE673529 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
RPA1_SCHPO	2.130e-28	45.80	DNA-directed RNA polymerase I subunit rpa1 OS=Schi...	[more]
RPA1_DROME	3.400e-26	42.76	DNA-directed RNA polymerase I subunit RPA1 OS=Dros...	[more]
RPA1_DICDI	6.412e-25	42.62	DNA-directed RNA polymerase I subunit rpa1 OS=Dict...	[more]
RPA1_YEAST	1.866e-24	45.45	DNA-directed RNA polymerase I subunit RPA1 OS=Sacc...	[more]
RPA1_MOUSE	9.259e-24	42.11	DNA-directed RNA polymerase I subunit RPA1 OS=Mus ...	[more]
RPA1_RAT	1.209e-23	42.11	DNA-directed RNA polymerase I subunit RPA1 OS=Ratt...	[more]
RPA1_HUMAN	4.595e-23	41.35	DNA-directed RNA polymerase I subunit RPA1 OS=Homo...	[more]
RPOA2_PYRAB	1.096e-16	36.75	DNA-directed RNA polymerase subunit A'' OS=Pyrococ...	[more]
RPOA2_HALMO	4.166e-16	44.68	DNA-directed RNA polymerase subunit A'' OS=Halococ...	[more]
RPOA2_PYRHO	5.441e-16	36.75	DNA-directed RNA polymerase subunit A'' OS=Pyrococ...	[more]

BLAST of FE673529 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SL65_RICCO	6.543e-64	65.36	DNA-directed RNA polymerase OS=Ricinus communis GN...	[more]
D7TI03_VITVI	2.749e-62	65.36	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9HA71_POPTR	1.045e-61	64.25	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q9SVY0_ARATH	4.700e-54	57.54	DNA-directed RNA polymerase OS=Arabidopsis thalian...	[more]
D7LVV3_ARALY	4.700e-54	57.54	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q69X40_ORYSJ	4.886e-35	46.93	DNA-directed RNA polymerase OS=Oryza sativa subsp....	[more]
Q0DAZ6_ORYSJ	4.886e-35	46.93	Os06g0612200 protein OS=Oryza sativa subsp. japoni...	[more]
C0JAC0_ORYCO	5.403e-34	58.68	DNA-directed RNA polymerase OS=Oryza coarctata PE=...	[more]
C5YUX4_SORBI	7.801e-33	44.13	Putative uncharacterized protein Sb09g027230 OS=So...	[more]
B9FU47_ORYSJ	1.127e-31	58.68	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of FE673529 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT3G57660.1	3.759e-56	57.54	\| Symbols: NRPA1 \| nuclear RNA polymerase A1 \| chr...	[more]
AT5G60040.1	2.087e-14	32.62	\| Symbols: NRPC1 \| nuclear RNA polymerase C1 \| chr...	[more]
AT5G60040.2	2.725e-14	38.61	\| Symbols: NRPC1 \| nuclear RNA polymerase C1 \| chr...	[more]
AT4G35800.1	4.072e-10	27.35	\| Symbols: NRPB1, RPB1, RNA_POL_II_LSRNA_POL_II_LS...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007081	RNA polymerase Rpb1, domain 5	PFAM	PF04998	RNA_pol_Rpb1_5	coord: 44..154 score: 1.8
IPR015699	DNA-directed RNA pol I, largest subunit	PANTHER	PTHR19376:SF11	DNA-DIRECTED RNA POLYMERASE I LARGEST SUBUNIT	coord: 35..183 score: 3.1
None	No IPR available	PANTHER	PTHR19376	DNA-DIRECTED RNA POLYMERASE	coord: 35..183 score: 3.1
None	No IPR available	SUPERFAMILY	64484	beta and beta-prime subunits of DNA dependent RNA-polymerase	coord: 54..183 score: 1.8

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE673529 ID=FE673529; Name=FE673529; organism=Cicer arietinum; type=EST; length=552bp
TGCAGGAATTCGAATTCCAATTCCTCAGATTGCTCAGAAAACAGCCGAGA
AGGTTTGTATCCAGAACTTTGGAAAAGTTGGTCAATGTCAAGCTATTACA
TGTAAAGAAAGTGGTGTAATCTACTATGGTGAAGATGATAGTAAAAGAGA
AGAAATTCCCTCATCAGTGAAAGAGAAAATACCAGCACTTCAAACATCTG
GCATACATTTTAAATCATTTTGGGAGATGCAAGATGATCTTAATGTTAGG
TATGTTTATTCAAACAACGTGCATGCTATTCTAAGCACTTACGGGGTGGA
AGCAGCCAAAGAAACCATCATCAGGGAAGTACAAAACGTTTTCAAGTCTT
ATGGAATATCGGTAAATATCCGACACTTGATGCTTATTGCTGACTTTATG
ACACACTCCGGTTGCTATCGCCCTATGAGCAGAAAGGGGAGCATAGCAGA
TTCTACATCCCCGTTCCTCAAAATTTGCTTCGAGACAGCATCAAATTTCA
TTGTTGAAGCAGCTTGTCACGGGCAGGTGGACAATTTGGATTCTCCTTCT
GC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0003899	DNA-directed 5'-3' RNA polymerase activity
GO:0008270	zinc ion binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006350	transcription

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **INTERPRO**
Term	Definition
IPR007081	RNA_pol_Rpb1_5
IPR015699	DNA-dir_RNA_pol1_lsu_N

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