GR391751

EST Overview


Name	GR391751
Unique Name	GR391751
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	259

Libraries


Library Name	Type
LIBEST_024778 ICC1882 field drought stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR391751 vs. SwissProt
Match: MTBC_ARATH (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Arabidopsis thaliana GN=At5g53850 PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 6.288e-18
Identity = 42/50 (84.00%), Postives = 46/50 (92.00%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            P++I+FVTDVYQEA AAKAAGLE IISIRPGN PLPENHGFKTV SFS+I
Sbjct:  458 PAEIMFVTDVYQEAVAAKAAGLEAIISIRPGNAPLPENHGFKTVTSFSQI 507

[Close]

BLAST of GR391751 vs. SwissProt
Match: MTBC_ORYSJ (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Oryza sativa subsp. japonica GN=Os11g0484000 PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 1.401e-17
Identity = 40/50 (80.00%), Postives = 46/50 (92.00%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            P+ ILF+TDV+QEA AAK+AG EVIISIRPGN PLPENHGF+T+KSFSEI
Sbjct:  469 PAQILFITDVFQEAVAAKSAGFEVIISIRPGNAPLPENHGFRTIKSFSEI 518

[Close]

BLAST of GR391751 vs. SwissProt
Match: MTBC_ORYSI (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Oryza sativa subsp. indica GN=OsI_36120 PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 1.401e-17
Identity = 40/50 (80.00%), Postives = 46/50 (92.00%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            P+ ILF+TDV+QEA AAK+AG EVIISIRPGN PLPENHGF+T+KSFSEI
Sbjct:  469 PAQILFITDVFQEAVAAKSAGFEVIISIRPGNAPLPENHGFRTIKSFSEI 518

[Close]

BLAST of GR391751 vs. SwissProt
Match: MTBC_POPTR (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Populus trichocarpa GN=POPTRDRAFT_827604 PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.830e-17
Identity = 41/50 (82.00%), Postives = 46/50 (92.00%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            PSDILFVTDV+QEA AAK AGL+V+ISIRPGN PLPENHGFKT+ SF+EI
Sbjct:  455 PSDILFVTDVFQEAFAAKGAGLDVMISIRPGNAPLPENHGFKTITSFAEI 504

[Close]

BLAST of GR391751 vs. SwissProt
Match: MTBC_MAIZE (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 4.076e-17
Identity = 40/50 (80.00%), Postives = 45/50 (90.00%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            PS ILF+TDV+QEA AAK AG EVIISIRPGN PLP+NHGF+T+KSFSEI
Sbjct:  468 PSQILFITDVFQEAIAAKNAGFEVIISIRPGNAPLPDNHGFRTIKSFSEI 517

[Close]

BLAST of GR391751 vs. SwissProt
Match: MTBC_SORBI (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Sorghum bicolor GN=SORBIDRAFT_0019s002010 PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 9.080e-17
Identity = 39/50 (78.00%), Postives = 44/50 (88.00%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            PS ILF+TDV+QEA AAK  G EVIISIRPGN PLP+NHGF+T+KSFSEI
Sbjct:  468 PSQILFITDVFQEAVAAKNTGFEVIISIRPGNAPLPDNHGFRTIKSFSEI 517

[Close]

BLAST of GR391751 vs. SwissProt
Match: MTBC_RICCO (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Ricinus communis GN=RCOM_0591060 PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.004e-15
Identity = 39/50 (78.00%), Postives = 42/50 (84.00%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            PS+ILFVTDV QEA AAK AGLE +ISIR GN PLPENHGFKT+ S SEI
Sbjct:  478 PSEILFVTDVVQEAVAAKGAGLEAVISIRQGNAPLPENHGFKTINSLSEI 527

[Close]

BLAST of GR391751 vs. SwissProt
Match: MTBC_PHYPA (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Physcomitrella patens GN=PHYPADRAFT_111231 PE=3 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 1.659e-10
Identity = 29/50 (58.00%), Postives = 36/50 (72.00%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            PS I F TDV  EA AAK AGL+ ++ +RPGN PLP +HGF+T KS  E+
Sbjct:  456 PSCITFATDVLAEAVAAKEAGLQAVLLLRPGNAPLPSDHGFRTAKSLLEL 505

[Close]

BLAST of GR391751 vs. SwissProt
Match: ENOPH_ZYGRC (Enolase-phosphatase E1 OS=Zygosaccharomyces rouxii (strain ATCC 2623 / CBS 732 / IFO 1130 / NBRC 1623 / NCYC 568) GN=UTR4 PE=3 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 4.517e-8
Identity = 23/47 (48.94%), Postives = 36/47 (76.60%), Query Frame = 3

Query:   12 ILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            +LF++D  +E  AA AAG++ +++IRPGN P+ ENHGF+TV+ F  +
Sbjct:  180 VLFISDNVKELEAAHAAGVKTLLAIRPGNPPVQENHGFRTVEKFDSL 226

[Close]

BLAST of GR391751 vs. SwissProt
Match: ENOPH_NAEGR (Enolase-phosphatase E1 OS=Naegleria gruberi GN=NAEGRDRAFT_60166 PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.716e-7
Identity = 25/49 (51.02%), Postives = 35/49 (71.43%), Query Frame = 3

Query:    6 SDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            SDILF+TD   EA AA+ AG++ ++S+RPG   LPE+H F+ V SF  +
Sbjct:  202 SDILFLTDNIGEAIAAREAGIDSVLSVRPGTMKLPEDHTFEAVTSFEHL 250

[Close]

BLAST of GR391751 vs. TrEMBL
Match: D7MTK8_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_331733 PE=4 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 9.077e-18
Identity = 45/50 (90.00%), Postives = 47/50 (94.00%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            PS+ILFVTDVYQEATAAKAAGLE IISIRPGN PLPENHGFKTV SFS+I
Sbjct:  470 PSEILFVTDVYQEATAAKAAGLEAIISIRPGNAPLPENHGFKTVTSFSQI 519

[Close]

BLAST of GR391751 vs. TrEMBL
Match: C5XFM9_SORBI (Putative uncharacterized protein Sb03g009140 OS=Sorghum bicolor GN=Sb03g009140 PE=4 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.893e-15
Identity = 40/50 (80.00%), Postives = 46/50 (92.00%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            PS ILF+TDVYQEATAA+AAGLEV+ISIRPGN PLPENH F+T+ SF+EI
Sbjct:  207 PSQILFLTDVYQEATAAQAAGLEVLISIRPGNAPLPENHRFETITSFAEI 256

[Close]

BLAST of GR391751 vs. TrEMBL
Match: A5BGD2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_005983 PE=4 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.893e-15
Identity = 39/50 (78.00%), Postives = 47/50 (94.00%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            PSD+LFVTDV+QEA AA+AAGLEV+ISIR GNGPLPENHGF+T+++F EI
Sbjct:  440 PSDMLFVTDVFQEAVAARAAGLEVVISIRLGNGPLPENHGFRTIETFLEI 489

[Close]

BLAST of GR391751 vs. TrEMBL
Match: Q7F2F8_ORYSJ (P0402A09.8 protein OS=Oryza sativa subsp. japonica GN=P0402A09.8 PE=4 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 2.472e-15
Identity = 39/50 (78.00%), Postives = 44/50 (88.00%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            PS ILF+TDVYQEA AAK AGLEVIIS+RPGN PLP+NHGF T+ SF+EI
Sbjct: 1071 PSQILFLTDVYQEAAAAKTAGLEVIISVRPGNAPLPDNHGFHTITSFAEI 1120

[Close]

BLAST of GR391751 vs. TrEMBL
Match: Q0JRH3_ORYSJ (Os01g0101200 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os01g0101200 PE=4 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 2.472e-15
Identity = 39/50 (78.00%), Postives = 44/50 (88.00%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            PS ILF+TDVYQEA AAK AGLEVIIS+RPGN PLP+NHGF T+ SF+EI
Sbjct:  255 PSQILFLTDVYQEAAAAKTAGLEVIISVRPGNAPLPDNHGFHTITSFAEI 304

[Close]

BLAST of GR391751 vs. TrEMBL
Match: B8ACR9_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_00013 PE=4 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 2.472e-15
Identity = 39/50 (78.00%), Postives = 44/50 (88.00%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            PS ILF+TDVYQEA AAK AGLEVIIS+RPGN PLP+NHGF T+ SF+EI
Sbjct:  258 PSQILFLTDVYQEAAAAKTAGLEVIISVRPGNAPLPDNHGFHTITSFAEI 307

[Close]

BLAST of GR391751 vs. TrEMBL
Match: B7EWK0_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_00011 PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 2.472e-15
Identity = 39/50 (78.00%), Postives = 44/50 (88.00%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            PS ILF+TDVYQEA AAK AGLEVIIS+RPGN PLP+NHGF T+ SF+EI
Sbjct:  258 PSQILFLTDVYQEAAAAKTAGLEVIISVRPGNAPLPDNHGFHTITSFAEI 307

[Close]

BLAST of GR391751 vs. TrEMBL
Match: B4FSI5_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 6.089e-14
Identity = 41/51 (80.39%), Postives = 46/51 (90.20%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLP-ENHGFKTVKSFSEI 152
            PS ILF+TDV QEATAA+AAGLEV+ISIRPGN PLP ENHGF TV+SF+EI
Sbjct:  221 PSQILFLTDVCQEATAAQAAGLEVLISIRPGNAPLPAENHGFGTVESFAEI 271

[Close]

BLAST of GR391751 vs. TrEMBL
Match: D0NSX3_PHYIN (Putative uncharacterized protein OS=Phytophthora infestans T30-4 GN=PITG_16113 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.127e-10
Identity = 29/48 (60.42%), Postives = 41/48 (85.42%), Query Frame = 3

Query:    9 DILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            D+LFVTDV +EA AA+AAGL+ ++S+RPGN PLPE+H F T+ SF+++
Sbjct:  574 DVLFVTDVIEEAQAAEAAGLDTVLSVRPGNKPLPESHHFPTICSFADL 621

[Close]

BLAST of GR391751 vs. TrEMBL
Match: A9TJZ6_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_146721 PE=4 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.027e-9
Identity = 29/50 (58.00%), Postives = 37/50 (74.00%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            PS I F TDV  EA AAK AGL+ ++ +RPGN PLP +HGF+T+KS  E+
Sbjct:  257 PSCITFATDVLAEAIAAKEAGLQAVLLLRPGNAPLPPDHGFRTIKSLHEL 306

[Close]

BLAST of GR391751 vs. TAIR peptide
Match: AT5G53850.2 (| Symbols: | haloacid dehalogenase-like hydrolase family protein | chr5:21861155-21864817 REVERSE LENGTH=507)

HSP 1 Score: 89.3521 bits (220), Expect = 5.098e-19
Identity = 42/50 (84.00%), Postives = 46/50 (92.00%), Query Frame = 3

Query:    3 PSDILFVTDVYQEATAAKAAGLEVIISIRPGNGPLPENHGFKTVKSFSEI 152
            P++I+FVTDVYQEA AAKAAGLE IISIRPGN PLPENHGFKTV SFS+I
Sbjct:  458 PAEIMFVTDVYQEAVAAKAAGLEAIISIRPGNAPLPENHGFKTVTSFSQI 507

The following BLAST results are available for this feature:

BLAST of GR391751 vs. SwissProt
BLAST of GR391751 vs. TrEMBL
BLAST of GR391751 vs. TAIR peptide

BLAST of GR391751 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
MTBC_ARATH	6.288e-18	84.00	Probable bifunctional methylthioribulose-1-phospha...	[more]
MTBC_ORYSJ	1.401e-17	80.00	Probable bifunctional methylthioribulose-1-phospha...	[more]
MTBC_ORYSI	1.401e-17	80.00	Probable bifunctional methylthioribulose-1-phospha...	[more]
MTBC_POPTR	1.830e-17	82.00	Probable bifunctional methylthioribulose-1-phospha...	[more]
MTBC_MAIZE	4.076e-17	80.00	Probable bifunctional methylthioribulose-1-phospha...	[more]
MTBC_SORBI	9.080e-17	78.00	Probable bifunctional methylthioribulose-1-phospha...	[more]
MTBC_RICCO	1.004e-15	78.00	Probable bifunctional methylthioribulose-1-phospha...	[more]
MTBC_PHYPA	1.659e-10	58.00	Probable bifunctional methylthioribulose-1-phospha...	[more]
ENOPH_ZYGRC	4.517e-8	48.94	Enolase-phosphatase E1 OS=Zygosaccharomyces rouxii...	[more]
ENOPH_NAEGR	1.716e-7	51.02	Enolase-phosphatase E1 OS=Naegleria gruberi GN=NAE...	[more]

BLAST of GR391751 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7MTK8_ARALY	9.077e-18	90.00	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
C5XFM9_SORBI	1.893e-15	80.00	Putative uncharacterized protein Sb03g009140 OS=So...	[more]
A5BGD2_VITVI	1.893e-15	78.00	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q7F2F8_ORYSJ	2.472e-15	78.00	P0402A09.8 protein OS=Oryza sativa subsp. japonica...	[more]
Q0JRH3_ORYSJ	2.472e-15	78.00	Os01g0101200 protein (Fragment) OS=Oryza sativa su...	[more]
B8ACR9_ORYSI	2.472e-15	78.00	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B7EWK0_ORYSJ	2.472e-15	78.00	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B4FSI5_MAIZE	6.089e-14	80.39	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
D0NSX3_PHYIN	3.127e-10	60.42	Putative uncharacterized protein OS=Phytophthora i...	[more]
A9TJZ6_PHYPA	2.027e-9	58.00	Predicted protein OS=Physcomitrella patens subsp. ...	[more]

BLAST of GR391751 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT5G53850.2	5.098e-19	84.00	\| Symbols: \| haloacid dehalogenase-like hydrolase...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010041	2,3-diketo-5-methylthio-1-phosphopentane phosphatase	PANTHER	PTHR20371	ENOLASE-PHOSPHATASE E-1	coord: 1..50 score: 1.7
None	No IPR available	SUPERFAMILY	56784	HAD-like	coord: 1..50 score: 2.1

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR391751 ID=GR391751; Name=GR391751; organism=Cicer arietinum; type=EST; length=259bp
AGCCATCAGATATTTTATTTGTCACCGACGTATATCAAGAAGCTACAGCT
GCAAAGGCAGCAGGTTTGGAGGTGATAATTTCTATTCGACCTGGAAATGG
ACCTCTCCCAGAAAATCATGGGTTCAAGACAGTCAAATCCTTCTCAGAGA
TCTAAGAGTACTTGCAGTCATCCTTGATGATATTGAACATTATGACTATT
AAAGGATATTGTAATGTCTACTTCTCGAAATGAAATAATGTATTTTGAAT
TTGTCCAAG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0019509	L-methionine salvage from methylthioadenosine

Vocabulary: **Molecular Function**
Term	Definition
GO:0043874	acireductone synthase activity
GO:0046872	metal ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR010041	2,3-diketo-5-methylthio-1-phosphopentane phosphatase

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