GR912547

EST Overview


Name	GR912547
Unique Name	GR912547
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	430

Libraries


Library Name	Type
LIBEST_024969 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR912547 vs. SwissProt
Match: E13B_PEA (Glucan endo-1,3-beta-glucosidase OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 244.202 bits (622), Expect = 1.671e-64
Identity = 117/127 (92.13%), Postives = 124/127 (97.64%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            VLNFYPSVKIKYIAVGNEVSP+GGSSWLAQYVLPATQN+YQAIRA+ LHDQIKV+T+IDMTLIGNSFPPSKGSFRS VRSYLDPFIGYLVYAGAPLLVNVYPYFS++GNPRDISLPYALFTSP VMV
Sbjct:  110 VLNFYPSVKIKYIAVGNEVSPVGGSSWLAQYVLPATQNVYQAIRAQGLHDQIKVTTAIDMTLIGNSFPPSKGSFRSDVRSYLDPFIGYLVYAGAPLLVNVYPYFSHIGNPRDISLPYALFTSPGVMV 236

[Close]

BLAST of GR912547 vs. SwissProt
Match: E13B_PHAVU (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 227.254 bits (578), Expect = 2.114e-59
Identity = 108/127 (85.04%), Postives = 118/127 (92.91%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            VLNF+PSVKIKYIAVGNEVSP+GGSSW AQYVLPA QN+YQA+RA+ LHDQIKVST+IDMTLIGNS+PPS+GSFR  VRSYLDP IGYL+YA APL VNVYPYFSY GNPRDISLPYALFTSPNV+V
Sbjct:   78 VLNFWPSVKIKYIAVGNEVSPVGGSSWYAQYVLPAVQNVYQAVRAQGLHDQIKVSTAIDMTLIGNSYPPSQGSFRGDVRSYLDPIIGYLLYASAPLHVNVYPYFSYSGNPRDISLPYALFTSPNVVV 204

[Close]

BLAST of GR912547 vs. SwissProt
Match: GUB_NICPL (Lichenase OS=Nicotiana plumbaginifolia GN=GN1 PE=2 SV=3)

HSP 1 Score: 181.03 bits (458), Expect = 1.737e-45
Identity = 85/127 (66.93%), Postives = 107/127 (84.25%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            V NF+P+VK +YIAVGNEVSP+ G+S L +Y+LPA +NI  AI +  L + IKVS+S+DMTLIGNSFPPS+GSFR+ VRS++DP IG++    +PLLVN+YPYFSY GNPRDISLPYALFT+PNV+V
Sbjct:  109 VRNFWPAVKFRYIAVGNEVSPVTGTSSLTRYLLPAMRNIRNAISSAGLQNNIKVSSSVDMTLIGNSFPPSQGSFRNDVRSFIDPIIGFVRRINSPLLVNIYPYFSYAGNPRDISLPYALFTAPNVVV 235

[Close]

BLAST of GR912547 vs. SwissProt
Match: E13B_HEVBR (Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis GN=HGN1 PE=1 SV=2)

HSP 1 Score: 176.407 bits (446), Expect = 4.279e-44
Identity = 84/128 (65.62%), Postives = 105/128 (82.03%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIG-GSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            V  F+ SV  +YIAVGNE+SP+  G++WLAQ+VLPA +NI+ AIR+  L DQIKVST+ID+TL+GNS+PPS G+FR  VRSYLDP IG+L    +PLL N+YPYF+Y  NPRDISLPYALFTSP+V+V
Sbjct:  113 VRGFWSSVLFRYIAVGNEISPVNRGTAWLAQFVLPAMRNIHDAIRSAGLQDQIKVSTAIDLTLVGNSYPPSAGAFRDDVRSYLDPIIGFLSSIRSPLLANIYPYFTYAYNPRDISLPYALFTSPSVVV 240

[Close]

BLAST of GR912547 vs. SwissProt
Match: E13B_SOLLC (Glucan endo-1,3-beta-glucosidase B OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 4.005e-42
Identity = 83/127 (65.35%), Postives = 102/127 (80.31%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            V +F+P VKIKYIAVGNE+SP+ G+S LA + +PA  NIY+AI    L + IKVSTS+DMTLIGNS+PPS+GSFR+ VR + DP +G+L    APLLVN+YPYFSY GNP  ISLPYALFT+PNV+V
Sbjct:  103 VRDFWPHVKIKYIAVGNEISPVTGTSNLAPFQVPALVNIYKAIGEAGLGNDIKVSTSVDMTLIGNSYPPSQGSFRNDVRWFTDPIVGFLRDTRAPLLVNIYPYFSYSGNPGQISLPYALFTAPNVVV 229

[Close]

BLAST of GR912547 vs. SwissProt
Match: E13B_NICPL (Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Nicotiana plumbaginifolia GN=GN2 PE=3 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 4.005e-42
Identity = 80/127 (62.99%), Postives = 102/127 (80.31%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            V +F+P VKIKYIAVGNE+SP+ G+S+L  ++ PA  NIY+AI    L + IKVSTS+DMTLIGNS+PPS+GSFR+  R ++DP +G+L    APLLVN+YPYFSY GNP  ISLPY+LFT+PNV+V
Sbjct:  111 VKDFWPDVKIKYIAVGNEISPVTGTSYLTSFLTPAMVNIYKAIGEAGLGNNIKVSTSVDMTLIGNSYPPSQGSFRNDARWFVDPIVGFLRDTRAPLLVNIYPYFSYSGNPGQISLPYSLFTAPNVVV 237

[Close]

BLAST of GR912547 vs. SwissProt
Match: E133_SOLTU (Glucan endo-1,3-beta-glucosidase, basic isoform 3 (Fragment) OS=Solanum tuberosum GN=GLUB3 PE=2 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 5.231e-42
Identity = 82/127 (64.57%), Postives = 101/127 (79.53%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            V +F+P VKIKYIAVGNE+SP+ G+S L  + +PA  NIY+AI    L + IKVSTS+DMTLIGNS+PPS+GSFR+ VR + DP +G+L    APLLVN+YPYFSY GNP  ISLPYALFT+PNV+V
Sbjct:   68 VKDFWPDVKIKYIAVGNEISPVTGTSSLTSFQVPALVNIYKAIGEAGLGNDIKVSTSVDMTLIGNSYPPSQGSFRNDVRWFTDPIVGFLRDTRAPLLVNIYPYFSYSGNPGQISLPYALFTAPNVVV 194

[Close]

BLAST of GR912547 vs. SwissProt
Match: E13F_TOBAC (Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GLB OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 6.832e-42
Identity = 80/127 (62.99%), Postives = 101/127 (79.53%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            V +F+P VKIKYIAVGNE+SP+ G+S+L  ++ PA  NIY+AI    L + IKVSTS+DMTLIGNS+PPS+GSFR+  R + DP +G+L    APLLVN+YPYFSY GNP  ISLPY+LFT+PNV+V
Sbjct:  111 VKDFWPDVKIKYIAVGNEISPVTGTSYLTSFLTPAMVNIYKAIGEAGLGNNIKVSTSVDMTLIGNSYPPSQGSFRNDARWFTDPIVGFLRDTRAPLLVNIYPYFSYSGNPGQISLPYSLFTAPNVVV 237

[Close]

BLAST of GR912547 vs. SwissProt
Match: E13B_TOBAC (Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Nicotiana tabacum PE=1 SV=2)

HSP 1 Score: 169.088 bits (427), Expect = 6.832e-42
Identity = 80/127 (62.99%), Postives = 101/127 (79.53%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            V +F+P VKIKYIAVGNE+SP+ G+S+L  ++ PA  NIY+AI    L + IKVSTS+DMTLIGNS+PPS+GSFR+  R + DP +G+L    APLLVN+YPYFSY GNP  ISLPY+LFT+PNV+V
Sbjct:  112 VKDFWPDVKIKYIAVGNEISPVTGTSYLTSFLTPAMVNIYKAIGEAGLGNNIKVSTSVDMTLIGNSYPPSQGSFRNDARWFTDPIVGFLRDTRAPLLVNIYPYFSYSGNPGQISLPYSLFTAPNVVV 238

[Close]

BLAST of GR912547 vs. SwissProt
Match: E132_SOLTU (Glucan endo-1,3-beta-glucosidase, basic isoform 2 OS=Solanum tuberosum GN=GLUB2 PE=2 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 6.832e-42
Identity = 81/127 (63.78%), Postives = 101/127 (79.53%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            V +F+P VKIKYIAVGNE+SP+ G+S L  + +PA  NIY+A+    L + IKVSTS+DMTLIGNS+PPS+GSFR+ VR + DP +G+L    APLLVN+YPYFSY GNP  ISLPYALFT+PNV+V
Sbjct:  103 VKDFWPDVKIKYIAVGNEISPVTGTSSLTSFQVPALVNIYKAVGEAGLGNDIKVSTSVDMTLIGNSYPPSQGSFRNDVRWFTDPIVGFLRDTRAPLLVNIYPYFSYSGNPGQISLPYALFTAPNVVV 229

[Close]

BLAST of GR912547 vs. TrEMBL
Match: Q9XFW9_CICAR (Glucan-endo-1,3-beta-glucosidase OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 255.758 bits (652), Expect = 1.055e-66
Identity = 126/127 (99.21%), Postives = 126/127 (99.21%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRS VRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV
Sbjct:  111 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSDVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 237

[Close]

BLAST of GR912547 vs. TrEMBL
Match: Q5RLY0_MEDSA (Acidic glucanase OS=Medicago sativa GN=Glu PE=2 SV=1)

HSP 1 Score: 245.743 bits (626), Expect = 1.092e-63
Identity = 119/127 (93.70%), Postives = 125/127 (98.43%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            VLNF+PSVKIKYIAVGNEVSP+GGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVST+IDMTLIGNSFPPSKGSFR+ VR+YLDPFIGYLVYAGAPLLVNVYPYFS+VGNPRDISLPYALFTSP VMV
Sbjct:  111 VLNFWPSVKIKYIAVGNEVSPVGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTAIDMTLIGNSFPPSKGSFRNDVRAYLDPFIGYLVYAGAPLLVNVYPYFSHVGNPRDISLPYALFTSPGVMV 237

[Close]

BLAST of GR912547 vs. TrEMBL
Match: A9YYK4_MEDSA (Acidic glucanase OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 245.743 bits (626), Expect = 1.092e-63
Identity = 119/127 (93.70%), Postives = 125/127 (98.43%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            VLNF+PSVKIKYIAVGNEVSP+GGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVST+IDMTLIGNSFPPSKGSFR+ VR+YLDPFIGYLVYAGAPLLVNVYPYFS+VGNPRDISLPYALFTSP VMV
Sbjct:  111 VLNFWPSVKIKYIAVGNEVSPVGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTAIDMTLIGNSFPPSKGSFRNDVRAYLDPFIGYLVYAGAPLLVNVYPYFSHVGNPRDISLPYALFTSPGVMV 237

[Close]

BLAST of GR912547 vs. TrEMBL
Match: Q2HU16_MEDTR (Glycoside hydrolase, family 17 OS=Medicago truncatula GN=MtrDRAFT_AC149491g10v2 PE=3 SV=1)

HSP 1 Score: 243.047 bits (619), Expect = 7.077e-63
Identity = 118/127 (92.91%), Postives = 124/127 (97.64%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            VLNF+PSVKIKYIAVGNEVSP+GGSSWLAQYVLPATQNIYQAIRA+ LHDQIKVST+IDMTLIGNSFPPSKGSFR+ VRSYLDPFIGYLVYAGAPLLVNVYPYFS+VGNPRDISLPYALFTSP VMV
Sbjct:  103 VLNFWPSVKIKYIAVGNEVSPVGGSSWLAQYVLPATQNIYQAIRAQGLHDQIKVSTAIDMTLIGNSFPPSKGSFRNDVRSYLDPFIGYLVYAGAPLLVNVYPYFSHVGNPRDISLPYALFTSPGVMV 229

[Close]

BLAST of GR912547 vs. TrEMBL
Match: Q40314_MEDSA (Acidic glucanase OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 241.506 bits (615), Expect = 2.059e-62
Identity = 117/127 (92.13%), Postives = 123/127 (96.85%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            VLNF+PSVKIKYIAVGNEVSP+GGSSWL QYVLPATQNIYQAIRAKNLHDQI VST+IDMTLIGNSFPPSKGSFR+ VR+YLDPFIGYLVYAGAPLLVNVYPYFS+VGNPRDISLPYALFTSP VMV
Sbjct:  111 VLNFWPSVKIKYIAVGNEVSPVGGSSWLGQYVLPATQNIYQAIRAKNLHDQILVSTAIDMTLIGNSFPPSKGSFRNDVRAYLDPFIGYLVYAGAPLLVNVYPYFSHVGNPRDISLPYALFTSPGVMV 237

[Close]

BLAST of GR912547 vs. TrEMBL
Match: Q4JGN7_PHAVU (1,3-beta-D-glucanase (Fragment) OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 1.058e-58
Identity = 109/127 (85.83%), Postives = 119/127 (93.70%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            VLNF+PSV+IKYIAVGNEVSP+GGSSW AQYVLPA QN+YQAIRA+ LHDQIKVST+IDMTLIGNS+PPS+GSFR  VRSYLDP IGYL+YA APLLVNVYPYFSY GNPRDISLPYALFTSPNV+V
Sbjct:   71 VLNFWPSVRIKYIAVGNEVSPVGGSSWYAQYVLPAVQNVYQAIRAQGLHDQIKVSTAIDMTLIGNSYPPSQGSFRGDVRSYLDPIIGYLLYASAPLLVNVYPYFSYSGNPRDISLPYALFTSPNVVV 197

[Close]

BLAST of GR912547 vs. TrEMBL
Match: Q2HU14_MEDTR (Glycoside hydrolase, family 17 OS=Medicago truncatula GN=MtrDRAFT_AC149491g12v2 PE=2 SV=1)

HSP 1 Score: 214.927 bits (546), Expect = 2.064e-54
Identity = 104/127 (81.89%), Postives = 114/127 (89.76%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            VLNFYPSVKIKYIAVGNEV+P+GGSS  A++VLPA QNIYQAIRAKNLHDQIKVST+IDMT+IG S+PPSKGSFRS VRSYLDP IGYLVYA APL  N+Y YFSY  NP+DISL YALFTSPNV+V
Sbjct:  106 VLNFYPSVKIKYIAVGNEVNPVGGSSQFAKFVLPAIQNIYQAIRAKNLHDQIKVSTAIDMTMIGTSYPPSKGSFRSDVRSYLDPIIGYLVYANAPLFANIYSYFSYKDNPKDISLQYALFTSPNVVV 232

[Close]

BLAST of GR912547 vs. TrEMBL
Match: Q2HU12_MEDTR (Glycoside hydrolase, family 17 OS=Medicago truncatula GN=MtrDRAFT_AC149491g14v2 PE=3 SV=1)

HSP 1 Score: 210.305 bits (534), Expect = 5.084e-53
Identity = 102/127 (80.31%), Postives = 112/127 (88.19%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            VLNFYPSVKIKYIAVGNEV+P+GGSS  A++VLPA QNIYQAIRAKN  DQIKVST+IDMT+IG S+PPSKGSFRS VRSYLDP IGYLVYA APL  N+Y YFSY  NP+DISL YALFTSPNV+V
Sbjct:  152 VLNFYPSVKIKYIAVGNEVNPVGGSSQFAKFVLPAIQNIYQAIRAKNFQDQIKVSTAIDMTMIGTSYPPSKGSFRSDVRSYLDPIIGYLVYANAPLFANIYSYFSYKDNPKDISLQYALFTSPNVVV 278

[Close]

BLAST of GR912547 vs. TrEMBL
Match: B7FJ23_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 210.305 bits (534), Expect = 5.084e-53
Identity = 102/127 (80.31%), Postives = 112/127 (88.19%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            VLNFYPSVKIKYIAVGNEV+P+GGSS  A++VLPA QNIYQAIRAKN  DQIKVST+IDMT+IG S+PPSKGSFRS VRSYLDP IGYLVYA APL  N+Y YFSY  NP+DISL YALFTSPNV+V
Sbjct:  106 VLNFYPSVKIKYIAVGNEVNPVGGSSQFAKFVLPAIQNIYQAIRAKNFQDQIKVSTAIDMTMIGTSYPPSKGSFRSDVRSYLDPIIGYLVYANAPLFANIYSYFSYKDNPKDISLQYALFTSPNVVV 232

[Close]

BLAST of GR912547 vs. TrEMBL
Match: Q2V8V2_9ROSA (Glucanase (Fragment) OS=Rosa roxburghii GN=PR2 PE=3 SV=1)

HSP 1 Score: 201.06 bits (510), Expect = 3.084e-50
Identity = 95/126 (75.40%), Postives = 112/126 (88.89%), Query Frame = -1

Query:   23 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVM 400
            VL+FYPSV+IKYIAVGNEVSP+ G + LAQ++LPA Q++YQA+RA NLHD+IKVST+ID TLIG S+PPS+G+FR  VR YLDP IGYLVYA APLL N+Y YFSYVGNPRDISLPYALFTSP+V+
Sbjct:   77 VLDFYPSVRIKYIAVGNEVSPVNGDTSLAQFLLPAMQHVYQAVRAANLHDRIKVSTAIDTTLIGVSYPPSQGAFRGDVRGYLDPIIGYLVYAKAPLLANIYTYFSYVGNPRDISLPYALFTSPSVV 202

[Close]

BLAST of GR912547 vs. TAIR peptide
Match: AT4G16260.1 (| Symbols: | Glycosyl hydrolase superfamily protein | chr4:9200180-9201441 REVERSE LENGTH=344)

HSP 1 Score: 170.629 bits (431), Expect = 2.874e-43
Identity = 84/127 (66.14%), Postives = 97/127 (76.38%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            VLN+YP+V  KYIAVGNEVSP  G       VLPA +N+Y A+R  NL D+IKVST+IDMTLIGNSFPPS G FR  VR Y+DP IG+L    + LL N+YPYFSYV NPRDISL YALFTSP+V+V
Sbjct:   99 VLNYYPAVSFKYIAVGNEVSPSNGGD----VVLPAMRNVYDALRGANLQDRIKVSTAIDMTLIGNSFPPSSGEFRGDVRWYIDPVIGFLTSTNSALLANIYPYFSYVDNPRDISLSYALFTSPSVVV 221

[Close]

BLAST of GR912547 vs. TAIR peptide
Match: AT3G57270.1 (| Symbols: BG1 | beta-1,3-glucanase 1 | chr3:21191336-21193118 REVERSE LENGTH=340)

HSP 1 Score: 119.398 bits (298), Expect = 7.599e-28
Identity = 62/127 (48.82%), Postives = 87/127 (68.50%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            V+N+   V+ +YI+VGNEV     S   AQ+++PA +NI +A+ A  L  +IKVSTS+DM ++  S+PPSKGSFR  V   ++P I +LV   +PLL+N+Y YFSY GN   I L YALFT+P+ +V
Sbjct:  103 VMNYANGVRFRYISVGNEVKI---SDSYAQFLVPAMENIDRAVLAAGLGGRIKVSTSVDMGVLRESYPPSKGSFRGDVMVVMEPIIRFLVSKNSPLLLNLYTYFSYAGNVGQIRLDYALFTAPSGIV 226

[Close]

BLAST of GR912547 vs. TAIR peptide
Match: AT3G57240.1 (| Symbols: BG3 | beta-1,3-glucanase 3 | chr3:21181916-21183045 REVERSE LENGTH=341)

HSP 1 Score: 108.612 bits (270), Expect = 1.341e-24
Identity = 62/123 (50.41%), Postives = 79/123 (64.23%), Query Frame = -1

Query:   20 YPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 388
            Y +V  +YI+VGNEV P   S   A +VLPA QNI +A+ +      IKVST+ID   I + FPPS G+F    RS++ P I +L    +PLLVN YPYFSY GN RDI L Y LFT+P+ +V
Sbjct:  114 YANVTFRYISVGNEVQP---SDQAASFVLPAMQNIERAVSSLG----IKVSTAIDTRGI-SGFPPSSGTFTPEFRSFIAPVISFLSSKQSPLLVNNYPYFSYTGNMRDIRLDYTLFTAPSTVV 228

[Close]

BLAST of GR912547 vs. TAIR peptide
Match: AT3G57260.1 (| Symbols: BGL2, PR2, BG2, PR-2 | beta-1,3-glucanase 2 | chr3:21188709-21189822 REVERSE LENGTH=339)

HSP 1 Score: 100.138 bits (248), Expect = 4.771e-22
Identity = 55/127 (43.31%), Postives = 81/127 (63.78%), Query Frame = -1

Query:   23 VLNFYPSVKIKYIAVGNEVSP-IGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVM 400
            V ++   V+ +YI VGNEV P +GG      ++L A QNI  A+    L  ++KVST+I      ++ PPS+G FR   +S+L+P IG+L    +PLLVN+YPYFSY+G+  +I L YALFT+ + +
Sbjct:  108 VQSYRDGVRFRYINVGNEVKPSVGG------FLLQAMQNIENAVSGAGL--EVKVSTAIATDTTTDTSPPSQGRFRDEYKSFLEPVIGFLASKQSPLLVNLYPYFSYMGDTANIHLDYALFTAQSTV 226

[Close]

BLAST of GR912547 vs. TAIR peptide
Match: AT5G56590.1 (| Symbols: | O-Glycosyl hydrolases family 17 protein | chr5:22907521-22909436 FORWARD LENGTH=506)

HSP 1 Score: 98.2117 bits (243), Expect = 1.813e-21
Identity = 50/130 (38.46%), Postives = 80/130 (61.54%), Query Frame = -1

Query:   20 VLNFYPSVKIKYIAVGNEVSP---IGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 400
            VL +YP+ KI YI VG E +    I  SS    +V+PA QN+  A+R   L  +IKVST++ + ++  SFPPS G+F S    +L P + +L    +P ++++YPY++Y  +P ++SL Y LF S + ++
Sbjct:  102 VLPYYPTTKITYITVGAESTDDPHINASS----FVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYFLRPMLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVI 227

[Close]

BLAST of GR912547 vs. TAIR peptide
Match: AT4G34480.1 (| Symbols: | O-Glycosyl hydrolases family 17 protein | chr4:16481147-16483988 REVERSE LENGTH=504)

HSP 1 Score: 97.0561 bits (240), Expect = 4.039e-21
Identity = 53/124 (42.74%), Postives = 77/124 (62.10%), Query Frame = -1

Query:   29 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPN 400
            VL FYP+ KI  I VGNE+  +     L   +LPA QN+ +A+ A +L  +IKVST   MT++G+S PPS GSF +G ++ L   + +L   G+P  +N YP+F+Y  +PR  +L + LF  PN
Sbjct:  102 VLPFYPASKIMLITVGNEIL-MSNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGLKGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLF-EPN 223

[Close]

BLAST of GR912547 vs. TAIR peptide
Match: AT5G20390.1 (| Symbols: | Glycosyl hydrolase superfamily protein | chr5:6892982-6894016 REVERSE LENGTH=344)

HSP 1 Score: 95.1301 bits (235), Expect = 1.535e-20
Identity = 48/124 (38.71%), Postives = 74/124 (59.68%), Query Frame = -1

Query:   20 FYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMV 391
            +   V I +IAVGNEV P      +  YVLP  +++   +++++L   I +ST++ MT +G S+PPS G F    R  L P + +L     P+LVN+YPYF+Y  +P +I L YA+F +  V+V
Sbjct:  113 YLADVNITFIAVGNEVIP----GEIGSYVLPVMKSLTNIVKSRSL--PILISTTVAMTNLGQSYPPSAGDFMPQAREQLTPVLKFLSQTNTPILVNIYPYFAYAADPINIQLDYAIFNTNKVVV 230

[Close]

BLAST of GR912547 vs. TAIR peptide
Match: AT2G26600.2 (| Symbols: | Glycosyl hydrolase superfamily protein | chr2:11317214-11318215 FORWARD LENGTH=294)

HSP 1 Score: 94.3597 bits (233), Expect = 2.618e-20
Identity = 43/120 (35.83%), Postives = 73/120 (60.83%), Query Frame = -1

Query:   41 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALF 400
            + +F P  +I+ IA+GNEV   GG S LA  +L A +N+Y A++  NL D ++++T+    +  +S+PPS   F+  V  ++ P + +    G+P  +N YP+ +Y  NP++I + YALF
Sbjct:   16 IQSFLPKTRIRGIAIGNEVLG-GGDSELAGALLGAAKNVYNALKKMNLEDTVQITTAHSQAVFSDSYPPSSCVFKENVVQFMKPLLEFFQQIGSPFCLNAYPFLAYTYNPKEIDINYALF 134

[Close]

BLAST of GR912547 vs. TAIR peptide
Match: AT2G26600.1 (| Symbols: | Glycosyl hydrolase superfamily protein | chr2:11316727-11318215 FORWARD LENGTH=388)

HSP 1 Score: 94.3597 bits (233), Expect = 2.618e-20
Identity = 43/120 (35.83%), Postives = 73/120 (60.83%), Query Frame = -1

Query:   41 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALF 400
            + +F P  +I+ IA+GNEV   GG S LA  +L A +N+Y A++  NL D ++++T+    +  +S+PPS   F+  V  ++ P + +    G+P  +N YP+ +Y  NP++I + YALF
Sbjct:  110 IQSFLPKTRIRGIAIGNEVLG-GGDSELAGALLGAAKNVYNALKKMNLEDTVQITTAHSQAVFSDSYPPSSCVFKENVVQFMKPLLEFFQQIGSPFCLNAYPFLAYTYNPKEIDINYALF 228

[Close]

BLAST of GR912547 vs. TAIR peptide
Match: AT2G16230.1 (| Symbols: | O-Glycosyl hydrolases family 17 protein | chr2:7035463-7038326 REVERSE LENGTH=503)

HSP 1 Score: 93.2041 bits (230), Expect = 5.832e-20
Identity = 56/130 (43.08%), Postives = 77/130 (59.23%), Query Frame = -1

Query:   11 VLNFYPSVKIKYIAVGNEVSPIGGSSWLAQYVLPATQNIYQAIRAKNLHDQIKVSTSIDMTLIGNSFPPSKGSFRSGVRSYLDPFIGYLVYAGAPLLVNVYPYFSYVGNPRDISLPYALFTSPNVMVPGR 400
            VL FYP+  I  I VGNEV  +     L   +LPA QN+ +A+ A +L  +IKVST   MT++GNS PPS GSF    ++ L   + +L   G+P  +N YP+F+Y  +PR  +L + LF  PN   PGR
Sbjct:  101 VLPFYPASNIILINVGNEVL-LSNDLNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVHAMTVLGNSEPPSAGSFAPSYQAGLKGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLF-QPN---PGR 225

The following BLAST results are available for this feature:

BLAST of GR912547 vs. SwissProt
BLAST of GR912547 vs. TrEMBL
BLAST of GR912547 vs. TAIR peptide

BLAST of GR912547 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
E13B_PEA	1.671e-64	92.13	Glucan endo-1,3-beta-glucosidase OS=Pisum sativum ...	[more]
E13B_PHAVU	2.114e-59	85.04	Glucan endo-1,3-beta-glucosidase, basic isoform OS...	[more]
GUB_NICPL	1.737e-45	66.93	Lichenase OS=Nicotiana plumbaginifolia GN=GN1 PE=2...	[more]
E13B_HEVBR	4.279e-44	65.63	Glucan endo-1,3-beta-glucosidase, basic vacuolar i...	[more]
E13B_SOLLC	4.005e-42	65.35	Glucan endo-1,3-beta-glucosidase B OS=Solanum lyco...	[more]
E13B_NICPL	4.005e-42	62.99	Glucan endo-1,3-beta-glucosidase, basic vacuolar i...	[more]
E133_SOLTU	5.231e-42	64.57	Glucan endo-1,3-beta-glucosidase, basic isoform 3 ...	[more]
E13F_TOBAC	6.832e-42	62.99	Glucan endo-1,3-beta-glucosidase, basic vacuolar i...	[more]
E13B_TOBAC	6.832e-42	62.99	Glucan endo-1,3-beta-glucosidase, basic vacuolar i...	[more]
E132_SOLTU	6.832e-42	63.78	Glucan endo-1,3-beta-glucosidase, basic isoform 2 ...	[more]

BLAST of GR912547 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9XFW9_CICAR	1.055e-66	99.21	Glucan-endo-1,3-beta-glucosidase OS=Cicer arietinu...	[more]
Q5RLY0_MEDSA	1.092e-63	93.70	Acidic glucanase OS=Medicago sativa GN=Glu PE=2 SV...	[more]
A9YYK4_MEDSA	1.092e-63	93.70	Acidic glucanase OS=Medicago sativa PE=2 SV=1	[more]
Q2HU16_MEDTR	7.077e-63	92.91	Glycoside hydrolase, family 17 OS=Medicago truncat...	[more]
Q40314_MEDSA	2.059e-62	92.13	Acidic glucanase OS=Medicago sativa PE=2 SV=1	[more]
Q4JGN7_PHAVU	1.058e-58	85.83	1,3-beta-D-glucanase (Fragment) OS=Phaseolus vulga...	[more]
Q2HU14_MEDTR	2.064e-54	81.89	Glycoside hydrolase, family 17 OS=Medicago truncat...	[more]
Q2HU12_MEDTR	5.084e-53	80.31	Glycoside hydrolase, family 17 OS=Medicago truncat...	[more]
B7FJ23_MEDTR	5.084e-53	80.31	Putative uncharacterized protein OS=Medicago trunc...	[more]
Q2V8V2_9ROSA	3.084e-50	75.40	Glucanase (Fragment) OS=Rosa roxburghii GN=PR2 PE=...	[more]

BLAST of GR912547 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT4G16260.1	2.874e-43	66.14	\| Symbols: \| Glycosyl hydrolase superfamily prote...	[more]
AT3G57270.1	7.599e-28	48.82	\| Symbols: BG1 \| beta-1,3-glucanase 1 \| chr3:21191...	[more]
AT3G57240.1	1.341e-24	50.41	\| Symbols: BG3 \| beta-1,3-glucanase 3 \| chr3:21181...	[more]
AT3G57260.1	4.771e-22	43.31	\| Symbols: BGL2, PR2, BG2, PR-2 \| beta-1,3-glucana...	[more]
AT5G56590.1	1.813e-21	38.46	\| Symbols: \| O-Glycosyl hydrolases family 17 prot...	[more]
AT4G34480.1	4.039e-21	42.74	\| Symbols: \| O-Glycosyl hydrolases family 17 prot...	[more]
AT5G20390.1	1.535e-20	38.71	\| Symbols: \| Glycosyl hydrolase superfamily prote...	[more]
AT2G26600.2	2.618e-20	35.83	\| Symbols: \| Glycosyl hydrolase superfamily prote...	[more]
AT2G26600.1	2.618e-20	35.83	\| Symbols: \| Glycosyl hydrolase superfamily prote...	[more]
AT2G16230.1	5.832e-20	43.08	\| Symbols: \| O-Glycosyl hydrolases family 17 prot...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000490	Glycoside hydrolase, family 17	PFAM	PF00332	Glyco_hydro_17	coord: 10..137 score: 8.9
IPR013781	Glycoside hydrolase, subgroup, catalytic core	GENE3D	3.20.20.80	no description	coord: 10..140 score: 2.1
IPR017853	Glycoside hydrolase, catalytic core	SUPERFAMILY	51445	(Trans)glycosidases	coord: 11..137 score: 1

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR912547 ID=GR912547; Name=GR912547; organism=Cicer arietinum; type=EST; length=430bp
TAGCGTGGTCGCGGCCGGGTACCATAACATTTGGGGAGGTGAAAAGAGCA
TAAGGTAGAGATATATCACGTGGGTTACCAACATAGCTAAAATAAGGGTA
AACATTAACAAGTAAAGGTGCACCCGCATATACCAAGTAACCAATAAAAG
GATCTAAATAAGACCTCACACCACTTCTAAATGAACCTTTAGATGGAGGA
AAAGAATTTCCAATAAGGGTCATATCAATAGAAGTTGAAACTTTAATTTG
GTCATGAAGATTTTTAGCTCTTATTGCTTGATATATATTTTGTGTGGCGG
GTAGAACATATTGTGCAAGCCAAGAAGAGCCACCAATTGGACTCACTTCA
TTACCAACTGCAATGTATTTGATTTTGACACTTGGATAGAAGTTAAGTAC
CTGCCCGGGCGGCCCTCGAAATCGAATTCC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0043169	cation binding

Vocabulary: **INTERPRO**
Term	Definition
IPR000490	Glyco_hydro_17
IPR013781	Glyco_hydro_catalytic_dom
IPR017853	Glycoside_hydrolase_SF

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