HO062657

EST Overview


Name	HO062657
Unique Name	HO062657
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	479

Libraries


Library Name	Type
LIBEST_026408 Chickpea drought stressed cDNA SSH library AB1	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of HO062657 vs. SwissProt
Match: METK2_SOLLC (S-adenosylmethionine synthase 2 OS=Solanum lycopersicum GN=SAM2 PE=2 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 4.354e-36
Identity = 71/76 (93.42%), Postives = 73/76 (96.05%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVD+YGTGKIPDKEIL IVKENFDFRPGMISINLDLLRGGN RFLKTAAYGHFGRDD DFTW
Sbjct:  305 QVSYAIGVPEPLSVFVDTYGTGKIPDKEILNIVKENFDFRPGMISINLDLLRGGNGRFLKTAAYGHFGRDDPDFTW 380

HSP 2 Score: 80.1073 bits (196), Expect = 5.534e-15
Identity = 36/40 (90.00%), Postives = 39/40 (97.50%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDP+SKV+
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPESKVA 41

[Close]

BLAST of HO062657 vs. SwissProt
Match: METK4_ARATH (S-adenosylmethionine synthase 4 OS=Arabidopsis thaliana GN=METK4 PE=1 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 5.687e-36
Identity = 71/76 (93.42%), Postives = 74/76 (97.37%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVDSYGTGKIPDKEIL+IVKE+FDFRPGMISINLDL RGGN RFLKTAAYGHFGRDDADFTW
Sbjct:  305 QVSYAIGVPEPLSVFVDSYGTGKIPDKEILEIVKESFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDADFTW 380

HSP 2 Score: 80.8777 bits (198), Expect = 3.244e-15
Identity = 36/40 (90.00%), Postives = 40/40 (100.00%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            E+FLFTSESVNEGHPDKLCDQISDA+LDACLEQDP+SKV+
Sbjct:    2 ESFLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVA 41

[Close]

BLAST of HO062657 vs. SwissProt
Match: METK2_BRAJU (S-adenosylmethionine synthase 2 OS=Brassica juncea GN=MSAMS2 PE=2 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 5.687e-36
Identity = 71/76 (93.42%), Postives = 74/76 (97.37%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVDSYGTGKIPDKEIL+IVKE+FDFRPGMISINLDL RGGN RFLKTAAYGHFGRDDADFTW
Sbjct:  305 QVSYAIGVPEPLSVFVDSYGTGKIPDKEILEIVKESFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDADFTW 380

HSP 2 Score: 80.8777 bits (198), Expect = 3.244e-15
Identity = 36/40 (90.00%), Postives = 40/40 (100.00%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            E+FLFTSESVNEGHPDKLCDQISDA+LDACLEQDP+SKV+
Sbjct:    2 ESFLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVA 41

[Close]

BLAST of HO062657 vs. SwissProt
Match: METK_MEDTR (S-adenosylmethionine synthase OS=Medicago truncatula GN=METK PE=3 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 7.428e-36
Identity = 70/76 (92.11%), Postives = 73/76 (96.05%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVDSYGTGKIPD+EILQIVKENFDFRPGMI+INLDL RGGN RFLKTAAYGHFGRDD DFTW
Sbjct:  306 QVSYAIGVPEPLSVFVDSYGTGKIPDREILQIVKENFDFRPGMITINLDLKRGGNSRFLKTAAYGHFGRDDPDFTW 381

HSP 2 Score: 84.3445 bits (207), Expect = 2.934e-16
Identity = 39/40 (97.50%), Postives = 40/40 (100.00%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKV+
Sbjct:    3 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVA 42

[Close]

BLAST of HO062657 vs. SwissProt
Match: METK_MEDFA (S-adenosylmethionine synthase OS=Medicago falcata GN=SAMS PE=2 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 7.428e-36
Identity = 71/76 (93.42%), Postives = 72/76 (94.74%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVDSYGTGKIPDKEIL IVKE FDFRPGMISINLDL RGGN RFLKTAAYGHFGRDDADFTW
Sbjct:  307 QVSYAIGVPEPLSVFVDSYGTGKIPDKEILNIVKETFDFRPGMISINLDLKRGGNNRFLKTAAYGHFGRDDADFTW 382

HSP 2 Score: 88.5817 bits (218), Expect = 1.556e-17
Identity = 41/43 (95.35%), Postives = 43/43 (100.00%), Query Frame = -2

Query:  221 MATETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 349
            MATETFL+TSESVNEGHPDKLCDQISDAVLDACLEQDPDSKV+
Sbjct:    1 MATETFLYTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVA 43

[Close]

BLAST of HO062657 vs. SwissProt
Match: METK5_POPTR (S-adenosylmethionine synthase 5 OS=Populus trichocarpa GN=METK5 PE=2 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 1.267e-35
Identity = 70/76 (92.11%), Postives = 73/76 (96.05%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVD+YGTGKIPDKEILQIVKE+FDFRPGMISINLDL RGGN RFLKTAAYGHFGRDD DFTW
Sbjct:  306 QVSYAIGVPEPLSVFVDTYGTGKIPDKEILQIVKESFDFRPGMISINLDLKRGGNSRFLKTAAYGHFGRDDPDFTW 381

HSP 2 Score: 82.0333 bits (201), Expect = 1.456e-15
Identity = 38/40 (95.00%), Postives = 39/40 (97.50%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQISDAVLDACL QDPDSKV+
Sbjct:    3 ETFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVA 42

[Close]

BLAST of HO062657 vs. SwissProt
Match: METK3_POPTR (S-adenosylmethionine synthase 3 OS=Populus trichocarpa GN=METK3 PE=2 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 1.267e-35
Identity = 70/76 (92.11%), Postives = 73/76 (96.05%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVD+YGTGKIPDKEILQIVKE+FDFRPGMISINLDL RGGN RFLKTAAYGHFGRDD DFTW
Sbjct:  306 QVSYAIGVPEPLSVFVDTYGTGKIPDKEILQIVKESFDFRPGMISINLDLKRGGNSRFLKTAAYGHFGRDDPDFTW 381

HSP 2 Score: 82.0333 bits (201), Expect = 1.456e-15
Identity = 38/40 (95.00%), Postives = 39/40 (97.50%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQISDAVLDACL QDPDSKV+
Sbjct:    3 ETFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVA 42

[Close]

BLAST of HO062657 vs. SwissProt
Match: METK_POPDE (S-adenosylmethionine synthase OS=Populus deltoides GN=METK PE=2 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 1.655e-35
Identity = 70/76 (92.11%), Postives = 72/76 (94.74%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVD+YGTGKIPDKEILQIVKE FDFRPGMISINLDL RGGN RFLKTAAYGHFGRDD DFTW
Sbjct:  306 QVSYAIGVPEPLSVFVDTYGTGKIPDKEILQIVKERFDFRPGMISINLDLKRGGNSRFLKTAAYGHFGRDDPDFTW 381

HSP 2 Score: 82.0333 bits (201), Expect = 1.456e-15
Identity = 38/40 (95.00%), Postives = 39/40 (97.50%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQISDAVLDACL QDPDSKV+
Sbjct:    3 ETFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVA 42

[Close]

BLAST of HO062657 vs. SwissProt
Match: METK5_VITVI (S-adenosylmethionine synthase 5 OS=Vitis vinifera GN=METK5 PE=3 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 1.655e-35
Identity = 70/76 (92.11%), Postives = 73/76 (96.05%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVDSYGTGKIPDKEIL+IVKENFDFRPGMI+INLDL RGGN RFLKTAAYGHFGRDD DFTW
Sbjct:  305 QVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMIAINLDLKRGGNGRFLKTAAYGHFGRDDPDFTW 380

HSP 2 Score: 82.0333 bits (201), Expect = 1.456e-15
Identity = 38/40 (95.00%), Postives = 39/40 (97.50%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQISDAVLDACL QDPDSKV+
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVA 41

[Close]

BLAST of HO062657 vs. SwissProt
Match: METK_SOLBR (S-adenosylmethionine synthase OS=Solanum brevidens GN=SAMS PE=2 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 2.161e-35
Identity = 69/76 (90.79%), Postives = 74/76 (97.37%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVD+YGTGKIPD+EIL+IVKENFDFRPGM+SINLDL RGGN RFLKTAAYGHFGRDDADFTW
Sbjct:  305 QVSYAIGVPEPLSVFVDTYGTGKIPDREILKIVKENFDFRPGMMSINLDLKRGGNGRFLKTAAYGHFGRDDADFTW 380

HSP 2 Score: 83.1889 bits (204), Expect = 6.537e-16
Identity = 47/87 (54.02%), Postives = 55/87 (63.22%), Query Frame = -2

Query:   83 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVSYAIGVPEPLSVFVDSYGTGKIPDKE-ILQIVKENFDFRPGMISINLD 340
            ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKV+        L +      T  I D E I++    N  F    + ++ D
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNLVMVFGEITTKAIVDYEKIVRDTCRNIGFVSDDVGLDAD 88

[Close]

BLAST of HO062657 vs. TrEMBL
Match: C3TS15_CICAR (S-adenosylmethionine synthase OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 1.014e-37
Identity = 79/92 (85.87%), Postives = 82/92 (89.13%), Query Frame = -2

Query:    2 ISDAVLDACLEQDPDSKVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 277
            ++ A+   CL Q     VSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW
Sbjct:  296 VASALARRCLVQ-----VSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 382

HSP 2 Score: 89.7373 bits (221), Expect = 9.871e-17
Identity = 42/43 (97.67%), Postives = 43/43 (100.00%), Query Frame = -2

Query:  221 MATETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 349
            MATETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKV+
Sbjct:    1 MATETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVA 43

[Close]

BLAST of HO062657 vs. TrEMBL
Match: Q76KV5_PEA (S-adenosylmethionine synthetase-2 OS=Pisum sativum GN=SAMS-2 PE=2 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 4.712e-35
Identity = 75/92 (81.52%), Postives = 79/92 (85.87%), Query Frame = -2

Query:    2 ISDAVLDACLEQDPDSKVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 277
            +++ +   CL Q     VSYAIGVPEPLSVFVDSYGTGKIPDKEIL IVKE FDFRPGMISINLDLLRGGN RFLKTAAYGHFGRDDADFTW
Sbjct:  245 VANGLARRCLVQ-----VSYAIGVPEPLSVFVDSYGTGKIPDKEILNIVKEAFDFRPGMISINLDLLRGGNGRFLKTAAYGHFGRDDADFTW 331

[Close]

BLAST of HO062657 vs. TrEMBL
Match: D7L6S2_ARALY (S-adenosylmethionine synthase 3 OS=Arabidopsis lyrata subsp. lyrata GN=MTO3 PE=4 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 8.037e-35
Identity = 71/76 (93.42%), Postives = 74/76 (97.37%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVDSYGTGKIPDKEIL+IVKE+FDFRPGMISINLDL RGGN RFLKTAAYGHFGRDDADFTW
Sbjct:  305 QVSYAIGVPEPLSVFVDSYGTGKIPDKEILEIVKESFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDADFTW 380

HSP 2 Score: 80.8777 bits (198), Expect = 4.585e-14
Identity = 36/40 (90.00%), Postives = 40/40 (100.00%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            E+FLFTSESVNEGHPDKLCDQISDA+LDACLEQDP+SKV+
Sbjct:    2 ESFLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVA 41

[Close]

BLAST of HO062657 vs. TrEMBL
Match: Q6J9X6_MEDSA (S-adenosylmethionine synthase (Fragment) OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 1.050e-34
Identity = 70/76 (92.11%), Postives = 74/76 (97.37%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVD+YGTGKIPDKEIL IVK+NFDFRPGMISINLDLLRGGN RFLKTAAYGHFGR+DADFTW
Sbjct:  305 QVSYAIGVPEPLSVFVDTYGTGKIPDKEILNIVKQNFDFRPGMISINLDLLRGGNGRFLKTAAYGHFGREDADFTW 380

HSP 2 Score: 80.8777 bits (198), Expect = 4.585e-14
Identity = 38/40 (95.00%), Postives = 39/40 (97.50%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQD DSKV+
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDVDSKVA 41

[Close]

BLAST of HO062657 vs. TrEMBL
Match: B9HXH1_POPTR (S-adenosylmethionine synthase OS=Populus trichocarpa GN=AdoMet3 PE=3 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 1.791e-34
Identity = 70/76 (92.11%), Postives = 73/76 (96.05%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVD+YGTGKIPDKEILQIVKE+FDFRPGMISINLDL RGGN RFLKTAAYGHFGRDD DFTW
Sbjct:  306 QVSYAIGVPEPLSVFVDTYGTGKIPDKEILQIVKESFDFRPGMISINLDLKRGGNNRFLKTAAYGHFGRDDPDFTW 381

HSP 2 Score: 82.0333 bits (201), Expect = 2.058e-14
Identity = 38/40 (95.00%), Postives = 39/40 (97.50%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQISDAVLDACL QDPDSKV+
Sbjct:    3 ETFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVA 42

[Close]

BLAST of HO062657 vs. TrEMBL
Match: B9HIH9_POPTR (S-adenosylmethionine synthase OS=Populus trichocarpa GN=AdoMet1 PE=3 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 1.791e-34
Identity = 70/76 (92.11%), Postives = 73/76 (96.05%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVD+YGTGKIPDKEILQIVKE+FDFRPGMISINLDL RGGN RFLKTAAYGHFGRDD DFTW
Sbjct:  306 QVSYAIGVPEPLSVFVDTYGTGKIPDKEILQIVKESFDFRPGMISINLDLKRGGNSRFLKTAAYGHFGRDDPDFTW 381

HSP 2 Score: 82.0333 bits (201), Expect = 2.058e-14
Identity = 38/40 (95.00%), Postives = 39/40 (97.50%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQISDAVLDACL QDPDSKV+
Sbjct:    3 ETFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVA 42

[Close]

BLAST of HO062657 vs. TrEMBL
Match: Q84RM7_SALMI (S-adenosylmethionine synthetase (Fragment) OS=Salvia miltiorrhiza PE=2 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 2.339e-34
Identity = 70/76 (92.11%), Postives = 73/76 (96.05%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVD++GTGKIPDKEILQIVKENFDFRPGMISINLDL RGGN RFLKTAAYGHFGRDD DFTW
Sbjct:  100 QVSYAIGVPEPLSVFVDTFGTGKIPDKEILQIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTW 175

[Close]

BLAST of HO062657 vs. TrEMBL
Match: B9RZZ0_RICCO (S-adenosylmethionine synthase OS=Ricinus communis GN=RCOM_1003190 PE=3 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 2.339e-34
Identity = 70/76 (92.11%), Postives = 73/76 (96.05%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVD+YGTGKIPDKEIL+IVKENFDFRPGMISINLDL RGGN RFLKTAAYGHFGRDD DFTW
Sbjct:  305 QVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTW 380

HSP 2 Score: 80.1073 bits (196), Expect = 7.821e-14
Identity = 35/40 (87.50%), Postives = 39/40 (97.50%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQ+SDA+LDACL QDPDS+V+
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQVSDAILDACLAQDPDSRVA 41

[Close]

BLAST of HO062657 vs. TrEMBL
Match: B9RN65_RICCO (S-adenosylmethionine synthase OS=Ricinus communis GN=RCOM_1343960 PE=3 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 3.054e-34
Identity = 69/76 (90.79%), Postives = 73/76 (96.05%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVDSYGTGKIPDKEIL+IVKENFDFRPGM++INLDL RGGN RFLKTAAYGHFGRDD DFTW
Sbjct:  305 QVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMMTINLDLKRGGNSRFLKTAAYGHFGRDDPDFTW 380

HSP 2 Score: 84.3445 bits (207), Expect = 4.147e-15
Identity = 39/40 (97.50%), Postives = 40/40 (100.00%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKV+
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVA 41

[Close]

BLAST of HO062657 vs. TrEMBL
Match: B9RFQ1_RICCO (S-adenosylmethionine synthase OS=Ricinus communis GN=RCOM_1436370 PE=3 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 5.210e-34
Identity = 69/76 (90.79%), Postives = 74/76 (97.37%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVD+YGTGKIPDKEIL+IVKE+FDFRPGMISINLDL RGGN RFLKTAAYGHFGRDD+DFTW
Sbjct:  315 QVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKESFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDSDFTW 390

HSP 2 Score: 83.5741 bits (205), Expect = 7.074e-15
Identity = 37/40 (92.50%), Postives = 40/40 (100.00%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPDSKV+
Sbjct:   12 ETFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPDSKVA 51

[Close]

BLAST of HO062657 vs. TAIR peptide
Match: AT3G17390.1 (| Symbols: MTO3, SAMS3, MAT4 | S-adenosylmethionine synthetase family protein | chr3:5952484-5953665 REVERSE LENGTH=393)

HSP 1 Score: 149.828 bits (377), Expect = 6.595e-37
Identity = 71/76 (93.42%), Postives = 74/76 (97.37%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVDSYGTGKIPDKEIL+IVKE+FDFRPGMISINLDL RGGN RFLKTAAYGHFGRDDADFTW
Sbjct:  305 QVSYAIGVPEPLSVFVDSYGTGKIPDKEILEIVKESFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDADFTW 380

HSP 2 Score: 80.8777 bits (198), Expect = 3.763e-16
Identity = 36/40 (90.00%), Postives = 40/40 (100.00%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            E+FLFTSESVNEGHPDKLCDQISDA+LDACLEQDP+SKV+
Sbjct:    2 ESFLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVA 41

[Close]

BLAST of HO062657 vs. TAIR peptide
Match: AT4G01850.2 (| Symbols: SAM-2, MAT2, SAM2, AtSAM2 | S-adenosylmethionine synthetase 2 | chr4:796298-797479 REVERSE LENGTH=393)

HSP 1 Score: 138.658 bits (348), Expect = 1.521e-33
Identity = 65/76 (85.53%), Postives = 70/76 (92.11%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVD+YGTG IPDKEIL+IVKE FDFRPGM++INLDL RGGN RF KTAAYGHFGRDD DFTW
Sbjct:  305 QVSYAIGVPEPLSVFVDTYGTGLIPDKEILKIVKETFDFRPGMMTINLDLKRGGNGRFQKTAAYGHFGRDDPDFTW 380

HSP 2 Score: 84.3445 bits (207), Expect = 3.403e-17
Identity = 39/40 (97.50%), Postives = 40/40 (100.00%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKV+
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVA 41

[Close]

BLAST of HO062657 vs. TAIR peptide
Match: AT4G01850.1 (| Symbols: SAM-2, MAT2, SAM2, AtSAM2 | S-adenosylmethionine synthetase 2 | chr4:796298-797479 REVERSE LENGTH=393)

HSP 1 Score: 138.658 bits (348), Expect = 1.521e-33
Identity = 65/76 (85.53%), Postives = 70/76 (92.11%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVD+YGTG IPDKEIL+IVKE FDFRPGM++INLDL RGGN RF KTAAYGHFGRDD DFTW
Sbjct:  305 QVSYAIGVPEPLSVFVDTYGTGLIPDKEILKIVKETFDFRPGMMTINLDLKRGGNGRFQKTAAYGHFGRDDPDFTW 380

HSP 2 Score: 84.3445 bits (207), Expect = 3.403e-17
Identity = 39/40 (97.50%), Postives = 40/40 (100.00%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKV+
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVA 41

[Close]

BLAST of HO062657 vs. TAIR peptide
Match: AT1G02500.2 (| Symbols: SAM1, SAM-1, MAT1, AtSAM1 | S-adenosylmethionine synthetase 1 | chr1:519037-520218 FORWARD LENGTH=393)

HSP 1 Score: 138.272 bits (347), Expect = 1.986e-33
Identity = 65/76 (85.53%), Postives = 71/76 (93.42%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVD+Y TG IPDKEIL+IVKE+FDFRPGM++INLDL RGGN RFLKTAAYGHFGRDD DFTW
Sbjct:  305 QVSYAIGVPEPLSVFVDTYETGLIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTW 380

HSP 2 Score: 84.3445 bits (207), Expect = 3.403e-17
Identity = 39/40 (97.50%), Postives = 40/40 (100.00%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKV+
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVA 41

[Close]

BLAST of HO062657 vs. TAIR peptide
Match: AT1G02500.1 (| Symbols: SAM1, SAM-1, MAT1, AtSAM1 | S-adenosylmethionine synthetase 1 | chr1:519037-520218 FORWARD LENGTH=393)

HSP 1 Score: 138.272 bits (347), Expect = 1.986e-33
Identity = 65/76 (85.53%), Postives = 71/76 (93.42%), Query Frame = -2

Query:    2 KVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 229
            +VSYAIGVPEPLSVFVD+Y TG IPDKEIL+IVKE+FDFRPGM++INLDL RGGN RFLKTAAYGHFGRDD DFTW
Sbjct:  305 QVSYAIGVPEPLSVFVDTYETGLIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTW 380

HSP 2 Score: 84.3445 bits (207), Expect = 3.403e-17
Identity = 39/40 (97.50%), Postives = 40/40 (100.00%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKV+
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVA 41

[Close]

BLAST of HO062657 vs. TAIR peptide
Match: AT2G36880.2 (| Symbols: MAT3 | methionine adenosyltransferase 3 | chr2:15479721-15480893 REVERSE LENGTH=390)

HSP 1 Score: 132.494 bits (332), Expect = 1.090e-31
Identity = 65/93 (69.89%), Postives = 76/93 (81.72%), Query Frame = -2

Query:    2 QISDAVLDACLEQDPDSKVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 280
            Q + +V+ A L +    +VSYAIGVPEPLSVFVD+Y TG IPDK+IL ++KE FDFRPGM++INLDL RGGN RF KTAAYGHFGRDD DFTW
Sbjct:  288 QAAKSVVAAGLARRCIVQVSYAIGVPEPLSVFVDTYKTGTIPDKDILVLIKEAFDFRPGMMAINLDLKRGGNFRFQKTAAYGHFGRDDPDFTW 380

HSP 2 Score: 82.4185 bits (202), Expect = 1.293e-16
Identity = 37/40 (92.50%), Postives = 40/40 (100.00%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQISDA+LDACLEQDP+SKV+
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVA 41

[Close]

BLAST of HO062657 vs. TAIR peptide
Match: AT2G36880.1 (| Symbols: MAT3 | methionine adenosyltransferase 3 | chr2:15479721-15480893 REVERSE LENGTH=390)

HSP 1 Score: 132.494 bits (332), Expect = 1.090e-31
Identity = 65/93 (69.89%), Postives = 76/93 (81.72%), Query Frame = -2

Query:    2 QISDAVLDACLEQDPDSKVSYAIGVPEPLSVFVDSYGTGKIPDKEILQIVKENFDFRPGMISINLDLLRGGNKRFLKTAAYGHFGRDDADFTW 280
            Q + +V+ A L +    +VSYAIGVPEPLSVFVD+Y TG IPDK+IL ++KE FDFRPGM++INLDL RGGN RF KTAAYGHFGRDD DFTW
Sbjct:  288 QAAKSVVAAGLARRCIVQVSYAIGVPEPLSVFVDTYKTGTIPDKDILVLIKEAFDFRPGMMAINLDLKRGGNFRFQKTAAYGHFGRDDPDFTW 380

HSP 2 Score: 82.4185 bits (202), Expect = 1.293e-16
Identity = 37/40 (92.50%), Postives = 40/40 (100.00%), Query Frame = -2

Query:  221 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVS 340
            ETFLFTSESVNEGHPDKLCDQISDA+LDACLEQDP+SKV+
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVA 41

The following BLAST results are available for this feature:

BLAST of HO062657 vs. SwissProt
BLAST of HO062657 vs. TrEMBL
BLAST of HO062657 vs. TAIR peptide

BLAST of HO062657 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
METK2_SOLLC	4.354e-36	93.42	S-adenosylmethionine synthase 2 OS=Solanum lycoper...	[more]
METK4_ARATH	5.687e-36	93.42	S-adenosylmethionine synthase 4 OS=Arabidopsis tha...	[more]
METK2_BRAJU	5.687e-36	93.42	S-adenosylmethionine synthase 2 OS=Brassica juncea...	[more]
METK_MEDTR	7.428e-36	92.11	S-adenosylmethionine synthase OS=Medicago truncatu...	[more]
METK_MEDFA	7.428e-36	93.42	S-adenosylmethionine synthase OS=Medicago falcata ...	[more]
METK5_POPTR	1.267e-35	92.11	S-adenosylmethionine synthase 5 OS=Populus trichoc...	[more]
METK3_POPTR	1.267e-35	92.11	S-adenosylmethionine synthase 3 OS=Populus trichoc...	[more]
METK_POPDE	1.655e-35	92.11	S-adenosylmethionine synthase OS=Populus deltoides...	[more]
METK5_VITVI	1.655e-35	92.11	S-adenosylmethionine synthase 5 OS=Vitis vinifera ...	[more]
METK_SOLBR	2.161e-35	90.79	S-adenosylmethionine synthase OS=Solanum brevidens...	[more]

BLAST of HO062657 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C3TS15_CICAR	1.014e-37	85.87	S-adenosylmethionine synthase OS=Cicer arietinum P...	[more]
Q76KV5_PEA	4.712e-35	81.52	S-adenosylmethionine synthetase-2 OS=Pisum sativum...	[more]
D7L6S2_ARALY	8.037e-35	93.42	S-adenosylmethionine synthase 3 OS=Arabidopsis lyr...	[more]
Q6J9X6_MEDSA	1.050e-34	92.11	S-adenosylmethionine synthase (Fragment) OS=Medica...	[more]
B9HXH1_POPTR	1.791e-34	92.11	S-adenosylmethionine synthase OS=Populus trichocar...	[more]
B9HIH9_POPTR	1.791e-34	92.11	S-adenosylmethionine synthase OS=Populus trichocar...	[more]
Q84RM7_SALMI	2.339e-34	92.11	S-adenosylmethionine synthetase (Fragment) OS=Salv...	[more]
B9RZZ0_RICCO	2.339e-34	92.11	S-adenosylmethionine synthase OS=Ricinus communis ...	[more]
B9RN65_RICCO	3.054e-34	90.79	S-adenosylmethionine synthase OS=Ricinus communis ...	[more]
B9RFQ1_RICCO	5.210e-34	90.79	S-adenosylmethionine synthase OS=Ricinus communis ...	[more]

BLAST of HO062657 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 7


Match Name	E-value	Identity	Description
AT3G17390.1	6.595e-37	93.42	\| Symbols: MTO3, SAMS3, MAT4 \| S-adenosylmethionin...	[more]
AT4G01850.2	1.521e-33	85.53	\| Symbols: SAM-2, MAT2, SAM2, AtSAM2 \| S-adenosylm...	[more]
AT4G01850.1	1.521e-33	85.53	\| Symbols: SAM-2, MAT2, SAM2, AtSAM2 \| S-adenosylm...	[more]
AT1G02500.2	1.986e-33	85.53	\| Symbols: SAM1, SAM-1, MAT1, AtSAM1 \| S-adenosylm...	[more]
AT1G02500.1	1.986e-33	85.53	\| Symbols: SAM1, SAM-1, MAT1, AtSAM1 \| S-adenosylm...	[more]
AT2G36880.2	1.090e-31	69.89	\| Symbols: MAT3 \| methionine adenosyltransferase 3...	[more]
AT2G36880.1	1.090e-31	69.89	\| Symbols: MAT3 \| methionine adenosyltransferase 3...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 29..54 score
None	No IPR available	GENE3D	3.30.300.10	no description	coord: 15..44 score: 1.5e-12coord: 45..118 score: 2.8
IPR002133	S-adenosylmethionine synthetase	PANTHER	PTHR11964	S-ADENOSYLMETHIONINE SYNTHETASE	coord: 44..118 score: 9.8
IPR022628	S-adenosylmethionine synthetase, N-terminal	PFAM	PF00438	S-AdoMet_synt_N	coord: 7..48 score: 4.3
IPR022630	S-adenosylmethionine synthetase, C-terminal	PFAM	PF02773	S-AdoMet_synt_C	coord: 26..118 score: 1.2
IPR022636	S-adenosylmethionine synthetase superfamily	SUPERFAMILY	55973	S-adenosylmethionine synthetase	coord: 43..118 score: 3.5e-21coord: 6..50 score: 5.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO062657 ID=HO062657; Name=HO062657; organism=Cicer arietinum; type=EST; length=479bp
CCCATGTGAAATCAGCATCATCTCTACCAAAATGTCCATAAGCAGCAGTC
TTCAAAAACCTTTTATTTCCACCTCTTAAAAGATCAAGGTTAATAGAGAT
CATTCCAGGCCTAAAATCAAAATTCTCCTTCACAATCTGAAGAATTTCCT
TATCAGGTATCTTTCCAGTTCCATAAGAATCAACAAAGACAGACAAAGGC
TCTGGAACACCAATAGCATAAGAAACTTTGCTGTCTGGATCTTGCTCAAG
GCAAGCATCAAGAACAGCATCAGAGATCTGGTCACATAGTTTGTCAGGGT
GTCCTTCGTTCACTGATTCAGATGTGAATAGGAAAGTTTCTGTTGCCATC
TTTGATTAATTATTACCTGAAAATACACATCATCAAAATTAAGTTAATTA
AATTAAGCATCCAAAATCAAATTCAGTCCAAGTTCATACACAAAATCAAC
CCATTCCTTTAGAGTATGATGTAGTACCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004478	methionine adenosyltransferase activity
GO:0005524	ATP binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006730	one-carbon metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR002133	S-AdoMet_synthetase
IPR022628	S-AdoMet_synt_N
IPR022630	S-AdoMet_synt_C
IPR022636	S-AdoMet_synthetase_sfam

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