HO062752

EST Overview


Name	HO062752
Unique Name	HO062752
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	490

Libraries


Library Name	Type
LIBEST_026409 Chickpea drought stressed cDNA SSH library AB2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO062752 vs. SwissProt
Match: ASNS2_PEA (Asparagine synthetase, root [glutamine-hydrolyzing] OS=Pisum sativum GN=AS2 PE=2 SV=3)

HSP 1 Score: 111.309 bits (277), Expect = 1.107e-45
Identity = 53/58 (91.38%), Postives = 54/58 (93.10%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LA QRLAIVDPASGDQPLFNED   IVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI
Sbjct:   45 YLAQQRLAIVDPASGDQPLFNEDNPSIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 102

HSP 2 Score: 92.4337 bits (228), Expect = 1.107e-45
Identity = 41/43 (95.35%), Postives = 43/43 (100.00%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGCSDPSRAKRVRVLELSRRLKHRGP+WSGLHQHGDC+
Sbjct:    3 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPEWSGLHQHGDCY 45

[Close]

BLAST of HO062752 vs. SwissProt
Match: ASNS1_LOTJA (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Lotus japonicus GN=AS1 PE=2 SV=2)

HSP 1 Score: 114.775 bits (286), Expect = 4.161e-45
Identity = 54/58 (93.10%), Postives = 56/58 (96.55%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHE+LRKQL NH FRTGSDCDVI
Sbjct:   45 YLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEELRKQLPNHQFRTGSDCDVI 102

HSP 2 Score: 87.0409 bits (214), Expect = 4.161e-45
Identity = 39/43 (90.70%), Postives = 42/43 (97.67%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGCSD ++AKRVRVLELSRRLKHRGPDWSGLHQHGDC+
Sbjct:    3 GILAVLGCSDFTQAKRVRVLELSRRLKHRGPDWSGLHQHGDCY 45

[Close]

BLAST of HO062752 vs. SwissProt
Match: ASNS2_LOTJA (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Lotus japonicus GN=AS2 PE=2 SV=2)

HSP 1 Score: 110.923 bits (276), Expect = 1.300e-43
Identity = 52/58 (89.66%), Postives = 55/58 (94.83%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            FLAHQRLAIVDPASGDQPLFNED+SIIVTVNGEI+NHE+LRKQL NH FRTG DCDVI
Sbjct:   45 FLAHQRLAIVDPASGDQPLFNEDQSIIVTVNGEIFNHEELRKQLPNHKFRTGCDCDVI 102

HSP 2 Score: 85.8853 bits (211), Expect = 1.300e-43
Identity = 40/43 (93.02%), Postives = 41/43 (95.35%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGCSD S+AKRVRVLELSRRLKHRGPDWSGLHQHGD F
Sbjct:    3 GILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLHQHGDNF 45

[Close]

BLAST of HO062752 vs. SwissProt
Match: ASNS_TRIVS (Asparagine synthetase [glutamine-hydrolyzing] OS=Triphysaria versicolor GN=AS PE=2 SV=3)

HSP 1 Score: 105.916 bits (263), Expect = 2.391e-42
Identity = 50/58 (86.21%), Postives = 52/58 (89.66%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LAHQRLAIVDPASGDQPLFNEDK I VTVNGEIYNHE+LR  L NH FRTGSDCDVI
Sbjct:   45 YLAHQRLAIVDPASGDQPLFNEDKRIAVTVNGEIYNHEELRALLPNHKFRTGSDCDVI 102

HSP 2 Score: 86.6557 bits (213), Expect = 2.391e-42
Identity = 38/43 (88.37%), Postives = 41/43 (95.35%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGCSD S+AKRVRVLELSRRLKHRGPDWSG+H HGDC+
Sbjct:    3 GILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGIHHHGDCY 45

[Close]

BLAST of HO062752 vs. SwissProt
Match: ASNS_ASPOF (Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis PE=2 SV=2)

HSP 1 Score: 105.531 bits (262), Expect = 4.059e-42
Identity = 46/58 (79.31%), Postives = 56/58 (96.55%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            FL+HQRLAI+DPASGDQPL+NEDKSI+VTVNGEIYNHE+LR++L +H +RTGSDC+VI
Sbjct:   45 FLSHQRLAIIDPASGDQPLYNEDKSIVVTVNGEIYNHEELRRRLPDHKYRTGSDCEVI 102

HSP 2 Score: 86.2705 bits (212), Expect = 4.059e-42
Identity = 40/43 (93.02%), Postives = 41/43 (95.35%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGCSD S+AKRVRVLELSRRLKHRGPDWSGL QHGDCF
Sbjct:    3 GILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLCQHGDCF 45

[Close]

BLAST of HO062752 vs. SwissProt
Match: ASNS_SANAU (Asparagine synthetase [glutamine-hydrolyzing] OS=Sandersonia aurantiaca GN=AND1 PE=2 SV=3)

HSP 1 Score: 107.842 bits (268), Expect = 4.064e-42
Identity = 49/58 (84.48%), Postives = 55/58 (94.83%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LAHQRLAI+DPASGDQPL+NEDK+IIVTVNGEIYNHE+LRK L  HTFRTGSDC+VI
Sbjct:   45 YLAHQRLAIIDPASGDQPLYNEDKTIIVTVNGEIYNHEELRKGLPGHTFRTGSDCEVI 102

HSP 2 Score: 83.9593 bits (206), Expect = 4.064e-42
Identity = 38/43 (88.37%), Postives = 40/43 (93.02%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGCSD S+AKRVRVLELSRRLKHRGPDWSGL  HGDC+
Sbjct:    3 GILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLDHHGDCY 45

[Close]

BLAST of HO062752 vs. SwissProt
Match: ASNS1_PEA (Asparagine synthetase, nodule [glutamine-hydrolyzing] OS=Pisum sativum GN=AS1 PE=2 SV=3)

HSP 1 Score: 106.686 bits (265), Expect = 6.892e-42
Identity = 51/58 (87.93%), Postives = 53/58 (91.38%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHE+LRKQL NH F T  DCDVI
Sbjct:   45 YLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEELRKQLPNHKFFTQCDCDVI 102

HSP 2 Score: 84.3445 bits (207), Expect = 6.892e-42
Identity = 38/41 (92.68%), Postives = 40/41 (97.56%), Query Frame = -3

Query:  180 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGD 302
            GILAVLGCSD S+AKRVR+LELSRRLKHRGPDWSGLHQHGD
Sbjct:    3 GILAVLGCSDDSQAKRVRILELSRRLKHRGPDWSGLHQHGD 43

[Close]

BLAST of HO062752 vs. SwissProt
Match: ASNS_ARATH (Asparagine synthetase [glutamine-hydrolyzing] OS=Arabidopsis thaliana GN=ASN1 PE=2 SV=2)

HSP 1 Score: 109.768 bits (273), Expect = 4.394e-41
Identity = 48/58 (82.76%), Postives = 56/58 (96.55%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LAHQRLA++DPASGDQPLFNEDK+I+VTVNGEIYNHE+LRK+L NH FRTGSDC+VI
Sbjct:   45 YLAHQRLAVIDPASGDQPLFNEDKTIVVTVNGEIYNHEELRKRLKNHKFRTGSDCEVI 102

HSP 2 Score: 78.5666 bits (192), Expect = 4.394e-41
Identity = 36/41 (87.80%), Postives = 40/41 (97.56%), Query Frame = -3

Query:  180 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGD 302
            GILAVLGCSD S+AKRVRVLELSRRL+HRGPDWSGL+Q+GD
Sbjct:    3 GILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGLYQNGD 43

[Close]

BLAST of HO062752 vs. SwissProt
Match: ASNS_BRAOL (Asparagine synthetase [glutamine-hydrolyzing] OS=Brassica oleracea PE=2 SV=2)

HSP 1 Score: 107.457 bits (267), Expect = 1.784e-39
Identity = 49/58 (84.48%), Postives = 53/58 (91.38%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LAHQRLAI+DP SGDQPLFNEDKSI+VTVNGEIYNHE+LRK L NH F TGSDCDVI
Sbjct:   45 YLAHQRLAIIDPDSGDQPLFNEDKSIVVTVNGEIYNHEELRKGLKNHKFHTGSDCDVI 102

HSP 2 Score: 75.485 bits (184), Expect = 1.784e-39
Identity = 34/40 (85.00%), Postives = 39/40 (97.50%), Query Frame = -3

Query:  183 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHG 302
            GILAVLGCSD S+AKRVRVLELSRRL+HRGPDWSG++Q+G
Sbjct:    3 GILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGIYQNG 42

[Close]

BLAST of HO062752 vs. SwissProt
Match: ASNS_MAIZE (Asparagine synthetase [glutamine-hydrolyzing] OS=Zea mays GN=ASN1 PE=2 SV=2)

HSP 1 Score: 99.7525 bits (247), Expect = 2.429e-35
Identity = 42/58 (72.41%), Postives = 53/58 (91.38%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LAHQRLAI+DP SGDQPL+NEDK+++VTVNGEIYNHE+L+ +L  H F+TGSDC+VI
Sbjct:   45 YLAHQRLAIIDPTSGDQPLYNEDKTVVVTVNGEIYNHEELKAKLKTHEFQTGSDCEVI 102

HSP 2 Score: 69.3218 bits (168), Expect = 2.429e-35
Identity = 31/43 (72.09%), Postives = 36/43 (83.72%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLG  + S AKR R++ELSRRL+HRGPDWSGLH H DC+
Sbjct:    3 GILAVLGVVEVSLAKRSRIIELSRRLRHRGPDWSGLHCHEDCY 45

[Close]

BLAST of HO062752 vs. TrEMBL
Match: B7FLS3_MEDTR (Putative uncharacterized protein (Fragment) OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 4.350e-47
Identity = 56/58 (96.55%), Postives = 57/58 (98.28%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            FLAHQRLAIVDPASGDQPLFNEDKS+IVTVNGEIYNHE LRKQLSNHTFRTGSDCDVI
Sbjct:   45 FLAHQRLAIVDPASGDQPLFNEDKSVIVTVNGEIYNHEALRKQLSNHTFRTGSDCDVI 102

HSP 2 Score: 93.9745 bits (232), Expect = 4.350e-47
Identity = 42/43 (97.67%), Postives = 43/43 (100.00%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGCSDP+RAKRVRVLELSRRLKHRGPDWSGLHQHGDCF
Sbjct:    3 GILAVLGCSDPTRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 45

[Close]

BLAST of HO062752 vs. TrEMBL
Match: Q9MB61_ASTSI (Asparagine synthetase OS=Astragalus sinicus GN=AsAS1 PE=2 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 7.982e-46
Identity = 55/58 (94.83%), Postives = 57/58 (98.28%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LAHQRLAIVDPASGDQPLFNEDKS+IVTVNGEIYNHEDLRKQL NHTFRTGSDCDVI
Sbjct:   45 YLAHQRLAIVDPASGDQPLFNEDKSVIVTVNGEIYNHEDLRKQLPNHTFRTGSDCDVI 102

HSP 2 Score: 89.7373 bits (221), Expect = 7.982e-46
Identity = 40/43 (93.02%), Postives = 42/43 (97.67%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGC+D SRAKRVRVLELSRRLKHRGPDWSGLHQHGDC+
Sbjct:    3 GILAVLGCADASRAKRVRVLELSRRLKHRGPDWSGLHQHGDCY 45

[Close]

BLAST of HO062752 vs. TrEMBL
Match: P93167_SOYBN (Asparagine synthetase 2 OS=Glycine max GN=AS2 PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 7.983e-46
Identity = 54/58 (93.10%), Postives = 56/58 (96.55%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            FLAHQRLAIVDPASGDQPLFNEDKS+IVTVNGEIYNHE+LRKQL NH FRTGSDCDVI
Sbjct:   45 FLAHQRLAIVDPASGDQPLFNEDKSVIVTVNGEIYNHEELRKQLPNHNFRTGSDCDVI 102

HSP 2 Score: 92.0485 bits (227), Expect = 7.983e-46
Identity = 42/43 (97.67%), Postives = 42/43 (97.67%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGCSD SRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF
Sbjct:    3 GILAVLGCSDDSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 45

[Close]

BLAST of HO062752 vs. TrEMBL
Match: Q9ZS23_PHAVU (Asparagine synthetase type II OS=Phaseolus vulgaris GN=as2 PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 6.643e-45
Identity = 53/58 (91.38%), Postives = 55/58 (94.83%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            FLAHQRLAIVDPASGDQPLFNEDKS+IVTVNGEIYNHE+LRKQL  H FRTGSDCDVI
Sbjct:   45 FLAHQRLAIVDPASGDQPLFNEDKSVIVTVNGEIYNHEELRKQLPGHKFRTGSDCDVI 102

HSP 2 Score: 91.6633 bits (226), Expect = 6.643e-45
Identity = 42/43 (97.67%), Postives = 42/43 (97.67%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGCSD SRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF
Sbjct:    3 GILAVLGCSDHSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 45

[Close]

BLAST of HO062752 vs. TrEMBL
Match: A9XS73_PHAVU (Asparagine synthetase OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 6.643e-45
Identity = 53/58 (91.38%), Postives = 55/58 (94.83%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            FLAHQRLAIVDPASGDQPLFNEDKS+IVTVNGEIYNHE+LRKQL  H FRTGSDCDVI
Sbjct:   45 FLAHQRLAIVDPASGDQPLFNEDKSVIVTVNGEIYNHEELRKQLPGHKFRTGSDCDVI 102

HSP 2 Score: 91.6633 bits (226), Expect = 6.643e-45
Identity = 42/43 (97.67%), Postives = 42/43 (97.67%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGCSD SRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF
Sbjct:    3 GILAVLGCSDHSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 45

[Close]

BLAST of HO062752 vs. TrEMBL
Match: Q84X69_9FABA (Asparagine synthetase OS=Securigera parviflora GN=AS2 PE=4 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.128e-44
Identity = 53/58 (91.38%), Postives = 56/58 (96.55%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            FLAHQRLAIVDPASGDQPLFNED+SIIVTVNGEIYNHE+LRKQ+ NH FRTGSDCDVI
Sbjct:   45 FLAHQRLAIVDPASGDQPLFNEDQSIIVTVNGEIYNHEELRKQMPNHKFRTGSDCDVI 102

HSP 2 Score: 90.5077 bits (223), Expect = 1.128e-44
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGCSD S+AKRVRVLELSRRLKHRGPDWSGLHQHGDCF
Sbjct:    3 GILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 45

[Close]

BLAST of HO062752 vs. TrEMBL
Match: Q8RVP2_9FABA (Asparagine synthetase (Fragment) OS=Securigera parviflora GN=As2 PE=2 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.131e-44
Identity = 53/58 (91.38%), Postives = 56/58 (96.55%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            FLAHQRLAIVDPASGDQPLFNED+SIIVTVNGEIYNHE+LRKQ+ NH FRTGSDCDVI
Sbjct:   45 FLAHQRLAIVDPASGDQPLFNEDQSIIVTVNGEIYNHEELRKQMPNHKFRTGSDCDVI 102

HSP 2 Score: 90.5077 bits (223), Expect = 1.131e-44
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGCSD S+AKRVRVLELSRRLKHRGPDWSGLHQHGDCF
Sbjct:    3 GILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 45

[Close]

BLAST of HO062752 vs. TrEMBL
Match: Q9SM55_PHAVU (Asparagine synthetase (Type-I) OS=Phaseolus vulgaris GN=as1 PE=2 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 9.386e-44
Identity = 53/58 (91.38%), Postives = 56/58 (96.55%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LAHQRLAIVDPASGDQPLFNEDK+I VTVNGEIYNHE+LRKQL NHTFRTGSDCDVI
Sbjct:   45 YLAHQRLAIVDPASGDQPLFNEDKTIAVTVNGEIYNHEELRKQLPNHTFRTGSDCDVI 102

HSP 2 Score: 87.0409 bits (214), Expect = 9.386e-44
Identity = 39/43 (90.70%), Postives = 42/43 (97.67%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGCSD S+AKRVRVLELSRRLKHRGPDWSGLHQ+GDC+
Sbjct:    3 GILAVLGCSDSSQAKRVRVLELSRRLKHRGPDWSGLHQYGDCY 45

[Close]

BLAST of HO062752 vs. TrEMBL
Match: B3TLZ2_ELAGV (Asparagine synthetase OS=Elaeis guineensis var. tenera PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 7.804e-43
Identity = 50/58 (86.21%), Postives = 56/58 (96.55%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            FLAHQRLAI+DPASGDQPL+NEDK+IIVTVNGEIYNHE+LR QLS+H FRTGSDC+VI
Sbjct:   45 FLAHQRLAIIDPASGDQPLYNEDKTIIVTVNGEIYNHEELRSQLSDHKFRTGSDCEVI 102

HSP 2 Score: 88.1965 bits (217), Expect = 7.804e-43
Identity = 40/43 (93.02%), Postives = 42/43 (97.67%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGCSD S+AKRVRVLELSRRLKHRGPDWSGLHQ+GDCF
Sbjct:    3 GILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLHQYGDCF 45

[Close]

BLAST of HO062752 vs. TrEMBL
Match: Q42792_SOYBN (Asparagine synthetase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 1.017e-42
Identity = 52/58 (89.66%), Postives = 57/58 (98.28%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LAHQRLAIVDPASGDQPLFNEDK+++VTVNGEIYNHE+LRKQL NHTFRTGSDCDVI
Sbjct:   45 YLAHQRLAIVDPASGDQPLFNEDKTVVVTVNGEIYNHEELRKQLPNHTFRTGSDCDVI 102

HSP 2 Score: 82.4185 bits (202), Expect = 1.017e-42
Identity = 38/41 (92.68%), Postives = 40/41 (97.56%), Query Frame = -3

Query:  180 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGD 302
            GILAVLGCSD S+AKRVRVLELSRRLKHRGPDWSGLHQ+GD
Sbjct:    3 GILAVLGCSDSSQAKRVRVLELSRRLKHRGPDWSGLHQYGD 43

[Close]

BLAST of HO062752 vs. TAIR peptide
Match: AT3G47340.1 (| Symbols: ASN1, DIN6, AT-ASN1 | glutamine-dependent asparagine synthase 1 | chr3:17438136-17441043 REVERSE LENGTH=584)

HSP 1 Score: 109.768 bits (273), Expect = 4.909e-42
Identity = 48/58 (82.76%), Postives = 56/58 (96.55%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LAHQRLA++DPASGDQPLFNEDK+I+VTVNGEIYNHE+LRK+L NH FRTGSDC+VI
Sbjct:   45 YLAHQRLAVIDPASGDQPLFNEDKTIVVTVNGEIYNHEELRKRLKNHKFRTGSDCEVI 102

HSP 2 Score: 78.5666 bits (192), Expect = 4.909e-42
Identity = 36/41 (87.80%), Postives = 40/41 (97.56%), Query Frame = -3

Query:  180 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGD 302
            GILAVLGCSD S+AKRVRVLELSRRL+HRGPDWSGL+Q+GD
Sbjct:    3 GILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGLYQNGD 43

[Close]

BLAST of HO062752 vs. TAIR peptide
Match: AT3G47340.2 (| Symbols: ASN1, DIN6, AT-ASN1 | glutamine-dependent asparagine synthase 1 | chr3:17438445-17441043 REVERSE LENGTH=512)

HSP 1 Score: 109.768 bits (273), Expect = 4.916e-42
Identity = 48/58 (82.76%), Postives = 56/58 (96.55%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LAHQRLA++DPASGDQPLFNEDK+I+VTVNGEIYNHE+LRK+L NH FRTGSDC+VI
Sbjct:   45 YLAHQRLAVIDPASGDQPLFNEDKTIVVTVNGEIYNHEELRKRLKNHKFRTGSDCEVI 102

HSP 2 Score: 78.5666 bits (192), Expect = 4.916e-42
Identity = 36/41 (87.80%), Postives = 40/41 (97.56%), Query Frame = -3

Query:  180 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGD 302
            GILAVLGCSD S+AKRVRVLELSRRL+HRGPDWSGL+Q+GD
Sbjct:    3 GILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGLYQNGD 43

[Close]

BLAST of HO062752 vs. TAIR peptide
Match: AT3G47340.3 (| Symbols: ASN1, DIN6, AT-ASN1 | glutamine-dependent asparagine synthase 1 | chr3:17438787-17441043 REVERSE LENGTH=456)

HSP 1 Score: 109.768 bits (273), Expect = 4.923e-42
Identity = 48/58 (82.76%), Postives = 56/58 (96.55%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LAHQRLA++DPASGDQPLFNEDK+I+VTVNGEIYNHE+LRK+L NH FRTGSDC+VI
Sbjct:   45 YLAHQRLAVIDPASGDQPLFNEDKTIVVTVNGEIYNHEELRKRLKNHKFRTGSDCEVI 102

HSP 2 Score: 78.5666 bits (192), Expect = 4.923e-42
Identity = 36/41 (87.80%), Postives = 40/41 (97.56%), Query Frame = -3

Query:  180 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGD 302
            GILAVLGCSD S+AKRVRVLELSRRL+HRGPDWSGL+Q+GD
Sbjct:    3 GILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGLYQNGD 43

[Close]

BLAST of HO062752 vs. TAIR peptide
Match: AT5G10240.1 (| Symbols: ASN3 | asparagine synthetase 3 | chr5:3212934-3216418 REVERSE LENGTH=578)

HSP 1 Score: 98.2117 bits (243), Expect = 4.266e-37
Identity = 44/58 (75.86%), Postives = 52/58 (89.66%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LAH+RLAIVDP SGDQPL+NEDK+I VTVNGEIYNH+ LR+ L +H FRTGSDC+VI
Sbjct:   45 YLAHERLAIVDPTSGDQPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVI 102

HSP 2 Score: 73.559 bits (179), Expect = 4.266e-37
Identity = 32/43 (74.42%), Postives = 38/43 (88.37%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGC D S+AKR R++ELSRRL+HRGPDWSGLH + DC+
Sbjct:    3 GILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCY 45

[Close]

BLAST of HO062752 vs. TAIR peptide
Match: AT5G10240.2 (| Symbols: ASN3 | asparagine synthetase 3 | chr5:3212934-3216418 REVERSE LENGTH=577)

HSP 1 Score: 98.2117 bits (243), Expect = 4.266e-37
Identity = 44/58 (75.86%), Postives = 52/58 (89.66%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LAH+RLAIVDP SGDQPL+NEDK+I VTVNGEIYNH+ LR+ L +H FRTGSDC+VI
Sbjct:   45 YLAHERLAIVDPTSGDQPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVI 102

HSP 2 Score: 73.559 bits (179), Expect = 4.266e-37
Identity = 32/43 (74.42%), Postives = 38/43 (88.37%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGC D S+AKR R++ELSRRL+HRGPDWSGLH + DC+
Sbjct:    3 GILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCY 45

[Close]

BLAST of HO062752 vs. TAIR peptide
Match: AT5G65010.2 (| Symbols: ASN2 | asparagine synthetase 2 | chr5:25969224-25972278 FORWARD LENGTH=579)

HSP 1 Score: 97.4413 bits (241), Expect = 7.238e-37
Identity = 42/58 (72.41%), Postives = 53/58 (91.38%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LAH+RLAI+DP SGDQPL+NEDK++ VTVNGEIYNH+ LR++L +H FRTGSDC+VI
Sbjct:   45 YLAHERLAIIDPTSGDQPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVI 102

HSP 2 Score: 73.559 bits (179), Expect = 7.238e-37
Identity = 32/43 (74.42%), Postives = 38/43 (88.37%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGC D S+AKR R++ELSRRL+HRGPDWSGLH + DC+
Sbjct:    3 GILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCY 45

[Close]

BLAST of HO062752 vs. TAIR peptide
Match: AT5G65010.1 (| Symbols: ASN2 | asparagine synthetase 2 | chr5:25969224-25972278 FORWARD LENGTH=578)

HSP 1 Score: 97.4413 bits (241), Expect = 7.239e-37
Identity = 42/58 (72.41%), Postives = 53/58 (91.38%), Query Frame = -1

Query:    2 FLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLSNHTFRTGSDCDVI 175
            +LAH+RLAI+DP SGDQPL+NEDK++ VTVNGEIYNH+ LR++L +H FRTGSDC+VI
Sbjct:   45 YLAHERLAIIDPTSGDQPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVI 102

HSP 2 Score: 73.559 bits (179), Expect = 7.239e-37
Identity = 32/43 (74.42%), Postives = 38/43 (88.37%), Query Frame = -3

Query:  174 GILAVLGCSDPSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCF 302
            GILAVLGC D S+AKR R++ELSRRL+HRGPDWSGLH + DC+
Sbjct:    3 GILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCY 45

The following BLAST results are available for this feature:

BLAST of HO062752 vs. SwissProt
BLAST of HO062752 vs. TrEMBL
BLAST of HO062752 vs. TAIR peptide

BLAST of HO062752 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ASNS2_PEA	1.107e-45	91.38	Asparagine synthetase, root [glutamine-hydrolyzing...	[more]
ASNS1_LOTJA	4.161e-45	93.10	Asparagine synthetase [glutamine-hydrolyzing] 1 OS...	[more]
ASNS2_LOTJA	1.300e-43	89.66	Asparagine synthetase [glutamine-hydrolyzing] 2 OS...	[more]
ASNS_TRIVS	2.391e-42	86.21	Asparagine synthetase [glutamine-hydrolyzing] OS=T...	[more]
ASNS_ASPOF	4.059e-42	79.31	Asparagine synthetase [glutamine-hydrolyzing] OS=A...	[more]
ASNS_SANAU	4.064e-42	84.48	Asparagine synthetase [glutamine-hydrolyzing] OS=S...	[more]
ASNS1_PEA	6.892e-42	87.93	Asparagine synthetase, nodule [glutamine-hydrolyzi...	[more]
ASNS_ARATH	4.394e-41	82.76	Asparagine synthetase [glutamine-hydrolyzing] OS=A...	[more]
ASNS_BRAOL	1.784e-39	84.48	Asparagine synthetase [glutamine-hydrolyzing] OS=B...	[more]
ASNS_MAIZE	2.429e-35	72.41	Asparagine synthetase [glutamine-hydrolyzing] OS=Z...	[more]

BLAST of HO062752 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FLS3_MEDTR	4.350e-47	96.55	Putative uncharacterized protein (Fragment) OS=Med...	[more]
Q9MB61_ASTSI	7.982e-46	94.83	Asparagine synthetase OS=Astragalus sinicus GN=AsA...	[more]
P93167_SOYBN	7.983e-46	93.10	Asparagine synthetase 2 OS=Glycine max GN=AS2 PE=2...	[more]
Q9ZS23_PHAVU	6.643e-45	91.38	Asparagine synthetase type II OS=Phaseolus vulgari...	[more]
A9XS73_PHAVU	6.643e-45	91.38	Asparagine synthetase OS=Phaseolus vulgaris PE=2 S...	[more]
Q84X69_9FABA	1.128e-44	91.38	Asparagine synthetase OS=Securigera parviflora GN=...	[more]
Q8RVP2_9FABA	1.131e-44	91.38	Asparagine synthetase (Fragment) OS=Securigera par...	[more]
Q9SM55_PHAVU	9.386e-44	91.38	Asparagine synthetase (Type-I) OS=Phaseolus vulgar...	[more]
B3TLZ2_ELAGV	7.804e-43	86.21	Asparagine synthetase OS=Elaeis guineensis var. te...	[more]
Q42792_SOYBN	1.017e-42	89.66	Asparagine synthetase OS=Glycine max PE=2 SV=1	[more]

BLAST of HO062752 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 7


Match Name	E-value	Identity	Description
AT3G47340.1	4.909e-42	82.76	\| Symbols: ASN1, DIN6, AT-ASN1 \| glutamine-depende...	[more]
AT3G47340.2	4.916e-42	82.76	\| Symbols: ASN1, DIN6, AT-ASN1 \| glutamine-depende...	[more]
AT3G47340.3	4.923e-42	82.76	\| Symbols: ASN1, DIN6, AT-ASN1 \| glutamine-depende...	[more]
AT5G10240.1	4.266e-37	75.86	\| Symbols: ASN3 \| asparagine synthetase 3 \| chr5:3...	[more]
AT5G10240.2	4.266e-37	75.86	\| Symbols: ASN3 \| asparagine synthetase 3 \| chr5:3...	[more]
AT5G65010.2	7.238e-37	72.41	\| Symbols: ASN2 \| asparagine synthetase 2 \| chr5:2...	[more]
AT5G65010.1	7.239e-37	72.41	\| Symbols: ASN2 \| asparagine synthetase 2 \| chr5:2...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 27..50 score
None	No IPR available	SEG	seg	seg	coord: 13..28 score
None	No IPR available	GENE3D	3.60.20.10	no description	coord: 15..74 score: 1.9
None	No IPR available	PANTHER	PTHR11772	ASPARAGINE SYNTHETASE	coord: 43..74 score: 1.2
None	No IPR available	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 15..74 score: 8.9
None	No IPR available	GENE3D	3.60.20.10	no description	coord: 14..59 score: 1
IPR017932	Glutamine amidotransferase, type II	PROFILE	PS51278	GATASE_TYPE_2	coord: 1..74 score: 16

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO062752 ID=HO062752; Name=HO062752; organism=Cicer arietinum; type=EST; length=490bp
CAATAACATCACAGTCACTTCCAGTTCTGAACGTGTGATTAGAGAGCTGT
TTCCTGAGATCTTCATGGTTGTAAATCTCTCCATTTACCGTGACGATGAT
GGATTTGTCTTCATTAAAGAGTGGTTGATCCCCAGAAGCAGGATCAACTA
TGGCTAACCGTTGGTGTGCCAAAAAAACAGTCACCATGTTGGTGGAGCCC
ACTCCAGTCAGGGCCACGGTGCTTCAATCTGCGAGAAAGCTCAAGAACGC
GAACCCTCTTAGCTCGAGAAGGATCGGAACAACCAAGCACGGCAAGTATA
CCCCCACATTGTAGTATATGTTTATGTGATGATGAAATTGATCAAAGAGA
AAGAGAACTTTGGAATAAAAAGAAAGAAGAAAATATAACTCAAGAAAGAA
ATGAAGAAAAGCACCACATATAGTAATGGTTTTGAGATTGAAGGCTTAAA
GCAATTCAGTATCCCCGCGTACCTCGGCCGCGACCACGCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR017932	GATase_2_dom

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