AJ005000

EST Overview


Name	AJ005000
Unique Name	AJ005000
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	501

Libraries


Library Name	Type
LIBEST_001193 chickpea mRNA CAN-5	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of AJ005000 vs. SwissProt
Match: BH135_ARATH (Transcription factor bHLH135 OS=Arabidopsis thaliana GN=BHLH135 PE=4 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 2.020e-21
Identity = 55/80 (68.75%), Postives = 69/80 (86.25%), Query Frame = 3

Query:    9 SSEITDAQITDLVAKLLRLIPELRPSR-SGKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLATTDSNSAQAAIIRSLL 245
            +S I++ QI DL+ KL +L+PELR SR S KVSAA+VLQ+TCNYI+NLHREVDDLS+RLS+LLA +D  +AQAA+IRSLL
Sbjct:   14 TSRISEDQINDLIIKLQQLLPELRDSRRSDKVSAARVLQDTCNYIRNLHREVDDLSERLSELLANSD--TAQAALIRSLL 91

[Close]

BLAST of AJ005000 vs. TrEMBL
Match: Q8GW32_ARATH (At1g26948 OS=Arabidopsis thaliana GN=At1g26945 PE=4 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.139e-26
Identity = 63/79 (79.75%), Postives = 71/79 (89.87%), Query Frame = 3

Query:    9 SSEITDAQITDLVAKLLRLIPELRPSRSGKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLATTDSNSAQAAIIRSLL 245
            SS I+D QI+DLV+KL  LIPELR  RS KVSA+KVLQETCNYI+NLHREVDDLSDRLS+LLA+TD NSA+AAIIRSLL
Sbjct:   14 SSRISDDQISDLVSKLQHLIPELRRRRSDKVSASKVLQETCNYIRNLHREVDDLSDRLSELLASTDDNSAEAAIIRSLL 92

[Close]

BLAST of AJ005000 vs. TrEMBL
Match: D7KPM5_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_472704 PE=4 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.139e-26
Identity = 63/79 (79.75%), Postives = 71/79 (89.87%), Query Frame = 3

Query:    9 SSEITDAQITDLVAKLLRLIPELRPSRSGKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLATTDSNSAQAAIIRSLL 245
            SS I+D QI+DLV+KL  LIPELR  RS KVSA+KVLQETCNYI+NLHREVDDLSDRLS+LLA+TD NSA+AAIIRSLL
Sbjct:   14 SSRISDDQISDLVSKLQHLIPELRRRRSDKVSASKVLQETCNYIRNLHREVDDLSDRLSELLASTDDNSAEAAIIRSLL 92

[Close]

BLAST of AJ005000 vs. TrEMBL
Match: B9S289_RICCO (Transcription regulator, putative OS=Ricinus communis GN=RCOM_0697210 PE=4 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 1.193e-25
Identity = 62/80 (77.50%), Postives = 72/80 (90.00%), Query Frame = 3

Query:   12 SEITDAQITDLVAKLLRLIPELRPSRSGKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLATTDSNSAQAAIIRSLLMK 251
            S I+D QI DLV+KL +LIPE+R  RS KVSA+KVLQETCNYIK+LHREVDDLSDRLS+LLA+TDS+SAQAAIIRSL M+
Sbjct:   14 SRISDDQIIDLVSKLQQLIPEIRNRRSDKVSASKVLQETCNYIKSLHREVDDLSDRLSELLASTDSDSAQAAIIRSLFME 93

[Close]

BLAST of AJ005000 vs. TrEMBL
Match: A5BU53_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_041983 PE=4 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 5.919e-25
Identity = 61/79 (77.22%), Postives = 70/79 (88.61%), Query Frame = 3

Query:   12 SEITDAQITDLVAKLLRLIPELRPSRSGKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLATTDSNSAQAAIIRSLLM 248
            S I+D QI DLV+KL +LIPE+R  RS KVSA+KVLQETCNYI+NLHREVDDLSDRLS LLA+TD++S QAAIIRSLLM
Sbjct:   14 SRISDDQIADLVSKLQQLIPEIRNRRSDKVSASKVLQETCNYIRNLHREVDDLSDRLSALLASTDTDSDQAAIIRSLLM 92

[Close]

BLAST of AJ005000 vs. TrEMBL
Match: B9R976_RICCO (Transcription regulator, putative OS=Ricinus communis GN=RCOM_1514610 PE=4 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 5.011e-24
Identity = 61/81 (75.31%), Postives = 72/81 (88.89%), Query Frame = 3

Query:    9 SSEITDAQITDLVAKLLRLIPELRPSRSGKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLATTDSNSAQAAIIRSLLMK 251
            SS ITD QI DLV+KL +L+PE+R  RS KVSAAKVLQETCNYI++LHREVDDLS+RLS+LLATTD  +AQAAIIR+LLM+
Sbjct:   13 SSRITDDQILDLVSKLQQLLPEIRNRRSDKVSAAKVLQETCNYIRSLHREVDDLSERLSELLATTD--TAQAAIIRNLLMQ 91

[Close]

BLAST of AJ005000 vs. TrEMBL
Match: B9RUP9_RICCO (Transcription regulator, putative OS=Ricinus communis GN=RCOM_0855340 PE=4 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.116e-23
Identity = 57/77 (74.03%), Postives = 68/77 (88.31%), Query Frame = 3

Query:   18 ITDAQITDLVAKLLRLIPELRPSRSGKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLATTDSNSAQAAIIRSLLM 248
            ITD QI DLV+KL +L+PE+R  R  KVSA+KVLQETCNYI+NLHREVDDLS+RLSQLLAT D++S +AAIIRSL+M
Sbjct:   14 ITDDQIIDLVSKLRQLLPEIRQRRPDKVSASKVLQETCNYIRNLHREVDDLSERLSQLLATIDADSPEAAIIRSLIM 90

[Close]

BLAST of AJ005000 vs. TrEMBL
Match: B9H3X1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1076150 PE=4 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.458e-23
Identity = 58/77 (75.32%), Postives = 67/77 (87.01%), Query Frame = 3

Query:   18 ITDAQITDLVAKLLRLIPELRPSRSGKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLATTDSNSAQAAIIRSLLM 248
            ITD QI  LV+KL +L+PE+R  RS KVSA+KVLQETCNYIKNLHREVDDLS+RLSQLLAT DS+S +A IIRSL+M
Sbjct:   14 ITDDQIIHLVSKLRQLLPEIRQRRSDKVSASKVLQETCNYIKNLHREVDDLSERLSQLLATIDSDSPEAEIIRSLIM 90

[Close]

BLAST of AJ005000 vs. TrEMBL
Match: A9PGK3_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=4 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.458e-23
Identity = 58/77 (75.32%), Postives = 67/77 (87.01%), Query Frame = 3

Query:   18 ITDAQITDLVAKLLRLIPELRPSRSGKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLATTDSNSAQAAIIRSLLM 248
            ITD QI  LV+KL +L+PE+R  RS KVSA+KVLQETCNYIKNLHREVDDLS+RLSQLLAT DS+S +A IIRSL+M
Sbjct:    8 ITDDQIIHLVSKLRQLLPEIRQRRSDKVSASKVLQETCNYIKNLHREVDDLSERLSQLLATIDSDSPEAEIIRSLIM 84

[Close]

BLAST of AJ005000 vs. TrEMBL
Match: C6T4Y3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 1.904e-23
Identity = 59/82 (71.95%), Postives = 72/82 (87.80%), Query Frame = 3

Query:    6 ASSEITDAQITDLVAKLLRLIPELRPSRSGKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLATTDSNSAQAAIIRSLLMK 251
            +S  ITD QI DLV+KL +L+PE+R  RS KVSA+KVLQETCNYI++LHREVDDLS+RLS+LLATTD  +AQAAIIR+LLM+
Sbjct:   13 SSRNITDDQINDLVSKLQQLLPEIRDRRSDKVSASKVLQETCNYIRSLHREVDDLSERLSELLATTD--TAQAAIIRNLLMQ 92

[Close]

BLAST of AJ005000 vs. TrEMBL
Match: D7SX91_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_108.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00011380001 PE=4 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 3.248e-23
Identity = 56/80 (70.00%), Postives = 71/80 (88.75%), Query Frame = 3

Query:    9 SSEITDAQITDLVAKLLRLIPELRPSRSGKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLATTDSNSAQAAIIRSLLM 248
            SS I+D QI +LV+KL +L+PE+R  RS KVSA+KVLQETCNYI++LHREVDDLS+RLS+LLAT D++S +AAIIRSL+M
Sbjct:   11 SSRISDDQIIELVSKLQQLLPEIRNRRSDKVSASKVLQETCNYIRSLHREVDDLSERLSRLLATVDADSPEAAIIRSLIM 90

[Close]

BLAST of AJ005000 vs. TAIR peptide
Match: AT1G26945.1 (| Symbols: KDR | basic helix-loop-helix (bHLH) DNA-binding superfamily protein | chr1:9351571-9352474 FORWARD LENGTH=94)

HSP 1 Score: 121.324 bits (303), Expect = 2.728e-28
Identity = 63/79 (79.75%), Postives = 71/79 (89.87%), Query Frame = 3

Query:    9 SSEITDAQITDLVAKLLRLIPELRPSRSGKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLATTDSNSAQAAIIRSLL 245
            SS I+D QI+DLV+KL  LIPELR  RS KVSA+KVLQETCNYI+NLHREVDDLSDRLS+LLA+TD NSA+AAIIRSLL
Sbjct:   14 SSRISDDQISDLVSKLQHLIPELRRRRSDKVSASKVLQETCNYIRNLHREVDDLSDRLSELLASTDDNSAEAAIIRSLL 92

[Close]

BLAST of AJ005000 vs. TAIR peptide
Match: AT5G15160.1 (| Symbols: BNQ2, BHLH134 | BANQUO 2 | chr5:4921487-4922553 REVERSE LENGTH=94)

HSP 1 Score: 102.834 bits (255), Expect = 1.004e-22
Identity = 53/81 (65.43%), Postives = 68/81 (83.95%), Query Frame = 3

Query:    6 ASSEITDAQITDLVAKLLRLIPELRPSR-SGKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLATTDSNSAQAAIIRSLL 245
            +SS I+D QITDL++KL + IPE+R +R S  VSA+KVLQETCNYI+NL++E DDLSDRL+QLL + D NS QAA+IRSL+
Sbjct:   12 SSSRISDDQITDLISKLRQSIPEIRQNRRSNTVSASKVLQETCNYIRNLNKEADDLSDRLTQLLESIDPNSPQAAVIRSLI 92

[Close]

BLAST of AJ005000 vs. TAIR peptide
Match: AT1G74500.1 (| Symbols: ATBS1, TMO7, BS1 | activation-tagged BRI1(brassinosteroid-insensitive 1)-suppressor 1 | chr1:27998298-27998668 REVERSE LENGTH=93)

HSP 1 Score: 101.679 bits (252), Expect = 2.237e-22
Identity = 55/80 (68.75%), Postives = 69/80 (86.25%), Query Frame = 3

Query:    9 SSEITDAQITDLVAKLLRLIPELRPSR-SGKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLATTDSNSAQAAIIRSLL 245
            +S I++ QI DL+ KL +L+PELR SR S KVSAA+VLQ+TCNYI+NLHREVDDLS+RLS+LLA +D  +AQAA+IRSLL
Sbjct:   14 TSRISEDQINDLIIKLQQLLPELRDSRRSDKVSAARVLQDTCNYIRNLHREVDDLSERLSELLANSD--TAQAALIRSLL 91

[Close]

BLAST of AJ005000 vs. TAIR peptide
Match: AT3G47710.1 (| Symbols: BNQ3, BHLH161 | BANQUO 3 | chr3:17590729-17591455 FORWARD LENGTH=92)

HSP 1 Score: 101.293 bits (251), Expect = 2.922e-22
Identity = 57/82 (69.51%), Postives = 70/82 (85.37%), Query Frame = 3

Query:    9 SSEITDAQITDLVAKLLRLIPELRPSR-SGKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLATTDSNSAQAAIIRSLLMK 251
            +S ITD QI DLV +L RL+PEL  +R SGKVSA++VLQETC+YI+NL +EVDDLS+RLSQLL +TD  SAQAA+IRSLLM+
Sbjct:   13 ASMITDEQINDLVLQLHRLLPELANNRRSGKVSASRVLQETCSYIRNLSKEVDDLSERLSQLLESTD--SAQAALIRSLLMQ 92

[Close]

BLAST of AJ005000 vs. TAIR peptide
Match: AT3G28857.1 (| Symbols: | basic helix-loop-helix (bHLH) DNA-binding family protein | chr3:10855781-10856313 REVERSE LENGTH=92)

HSP 1 Score: 99.7525 bits (247), Expect = 8.501e-22
Identity = 52/81 (64.20%), Postives = 66/81 (81.48%), Query Frame = 3

Query:    9 SSEITDAQITDLVAKLLRLIPELRPSR-SGKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLATTDSNSAQAAIIRSLLM 248
            +S I+D Q+ DLV+KL + +PE+   R S KVSA+KVLQETCNYI+ LHREVD+LSDRLSQLL + D +S +AA+IRSLLM
Sbjct:   12 ASRISDDQMIDLVSKLRQFLPEIHERRRSDKVSASKVLQETCNYIRKLHREVDNLSDRLSQLLDSVDEDSPEAAVIRSLLM 92

[Close]

BLAST of AJ005000 vs. TAIR peptide
Match: AT5G39860.1 (| Symbols: PRE1, BNQ1, BHLH136 | basic helix-loop-helix (bHLH) DNA-binding family protein | chr5:15957689-15958303 FORWARD LENGTH=92)

HSP 1 Score: 95.9005 bits (237), Expect = 1.228e-20
Identity = 49/82 (59.76%), Postives = 68/82 (82.93%), Query Frame = 3

Query:    6 ASSEITDAQITDLVAKLLRLIPEL-RPSRSGKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLATTDSNSAQAAIIRSLLM 248
            ++  I+D Q+ DLV+KL +++PE+ +  RS K SA+KVLQETCNYI+NL+REVD+LS+RLSQLL + D +S +AA+IRSLLM
Sbjct:   11 SAPRISDNQMIDLVSKLRQILPEIGQRRRSDKASASKVLQETCNYIRNLNREVDNLSERLSQLLESVDEDSPEAAVIRSLLM 92

The following BLAST results are available for this feature:

BLAST of AJ005000 vs. SwissProt
BLAST of AJ005000 vs. TrEMBL
BLAST of AJ005000 vs. TAIR peptide

BLAST of AJ005000 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 1


Match Name	E-value	Identity	Description
BH135_ARATH	2.020e-21	68.75	Transcription factor bHLH135 OS=Arabidopsis thalia...	[more]

BLAST of AJ005000 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q8GW32_ARATH	3.139e-26	79.75	At1g26948 OS=Arabidopsis thaliana GN=At1g26945 PE=...	[more]
D7KPM5_ARALY	3.139e-26	79.75	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9S289_RICCO	1.193e-25	77.50	Transcription regulator, putative OS=Ricinus commu...	[more]
A5BU53_VITVI	5.919e-25	77.22	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9R976_RICCO	5.011e-24	75.31	Transcription regulator, putative OS=Ricinus commu...	[more]
B9RUP9_RICCO	1.116e-23	74.03	Transcription regulator, putative OS=Ricinus commu...	[more]
B9H3X1_POPTR	1.458e-23	75.32	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PGK3_POPTR	1.458e-23	75.32	Putative uncharacterized protein OS=Populus tricho...	[more]
C6T4Y3_SOYBN	1.904e-23	71.95	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7SX91_VITVI	3.248e-23	70.00	Whole genome shotgun sequence of line PN40024, sca...	[more]

BLAST of AJ005000 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT1G26945.1	2.728e-28	79.75	\| Symbols: KDR \| basic helix-loop-helix (bHLH) DNA...	[more]
AT5G15160.1	1.004e-22	65.43	\| Symbols: BNQ2, BHLH134 \| BANQUO 2 \| chr5:4921487...	[more]
AT1G74500.1	2.237e-22	68.75	\| Symbols: ATBS1, TMO7, BS1 \| activation-tagged BR...	[more]
AT3G47710.1	2.922e-22	69.51	\| Symbols: BNQ3, BHLH161 \| BANQUO 3 \| chr3:1759072...	[more]
AT3G28857.1	8.501e-22	64.20	\| Symbols: \| basic helix-loop-helix (bHLH) DNA-bi...	[more]
AT5G39860.1	1.228e-20	59.76	\| Symbols: PRE1, BNQ1, BHLH136 \| basic helix-loop-...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001092	Helix-loop-helix DNA-binding domain	PFAM	PF00010	HLH	coord: 10..49 score: 3.9
IPR011598	Helix-loop-helix DNA-binding	GENE3D	4.10.280.10	no description	coord: 11..65 score: 4.8
IPR011598	Helix-loop-helix DNA-binding	SUPERFAMILY	47459	HLH, helix-loop-helix DNA-binding domain	coord: 10..82 score: 1.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AJ005000 ID=AJ005000; Name=AJ005000; organism=Cicer arietinum; type=EST; length=501bp
CTGGTGCTTCCTCTGAGATCACTGATGCTCAAATCACCGATCTCGTCGCT
AAATTACTACGACTTATCCCTGAGCTTCGCCCTAGCCGCTCCGGCAAGGT
TTCAGCAGCTAAGGTATTGCAAGAGACTTGCAACTACATAAAAAACTTGC
ATAGAGAAGTTGATGATTTAAGTGACCGATTATCTCAACTTTTGGCTACA
ACAGATTCCAACAGTGCTCAAGCAGCTATAATTAGGAGCTTACTTATGAA
ATAATGTCTAGTTATATATATAACTACTACTACTACTCTGCATTTATTTG
TCGTGTGCATGTCTCTGTCTCACTTTATGGTTATTCTCTTATCTGTGTAG
GGTTTGGTTTGTAGACCAAGGCAAAAGGGTTATACCTTTGTTAGTCCTTG
ATTAATTACTTTAATTCTATGTGACACTTTGTAGTCATTATGATTATGAT
ATGATCATCATCAGCTTTTATAGCTATAAATAAAGTGGACTACATTTTAA
T

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0030528	obsolete transcription regulator activity

Vocabulary: **Biological Process**
Term	Definition
GO:0045449	regulation of transcription

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **INTERPRO**
Term	Definition
IPR001092	Helix-loop-helix DNA-binding domain
IPR011598	bHLH_dom

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