CK148894

EST Overview


Name	CK148894
Unique Name	CK148894
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	718

Libraries


Library Name	Type
LIBEST_014674 Suppressive subtractive hybridization library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of CK148894 vs. SwissProt
Match: SYV_ARATH (Valyl-tRNA synthetase OS=Arabidopsis thaliana GN=VALRS PE=1 SV=2)

HSP 1 Score: 83.1889 bits (204), Expect = 6.744e-28
Identity = 38/62 (61.29%), Postives = 44/62 (70.97%), Query Frame = 3

Query:  396 NPTAAEKSWYEWWEKSKYF-GRC*QLQTTFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            +P   EKSWY WWEKS  F       +  F   LPPPNVTGALHIGHALT+AIEDT+IRW++
Sbjct:  141 SPATVEKSWYAWWEKSDLFKADAKSSKPPFVIVLPPPNVTGALHIGHALTSAIEDTIIRWKR 202

HSP 2 Score: 62.003 bits (149), Expect = 6.744e-28
Identity = 27/34 (79.41%), Postives = 31/34 (91.18%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQLT 679
            MSGYN LWVPG+DHAGIATQVVVEKK+MR+  +T
Sbjct:  203 MSGYNALWVPGVDHAGIATQVVVEKKIMRDRGMT 236

[Close]

BLAST of CK148894 vs. SwissProt
Match: SYVC_TAKRU (Valyl-tRNA synthetase OS=Takifugu rubripes GN=vars PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.136e-22
Identity = 34/77 (44.16%), Postives = 48/77 (62.34%), Query Frame = 3

Query:  372 VAAIGETDNPTAAEKSWYEWWEKSKYF----GRC*----QLQTTFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            V+ + ++ +P   E +WY WWEK  +F    GR        +  F  C+PPPNVTG+LH+GHALT AI+DT+ RW +
Sbjct:  242 VSPLPDSYSPQYVEAAWYPWWEKQGFFKPEFGRKSIGEQNPRGIFMMCIPPPNVTGSLHLGHALTNAIQDTLTRWHR 318

HSP 2 Score: 52.7582 bits (125), Expect = 1.136e-22
Identity = 25/30 (83.33%), Postives = 25/30 (83.33%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRE 667
            M G  TLW PG DHAGIATQVVVEKKLMRE
Sbjct:  319 MRGETTLWNPGCDHAGIATQVVVEKKLMRE 348

HSP 3 Score: 22.3274 bits (46), Expect = 1.136e-22
Identity = 9/17 (52.94%), Postives = 13/17 (76.47%), Query Frame = 1

Query:  649 KKVNA*KSTNQHDLGRK 699
            KK+   K T++HDLGR+
Sbjct:  343 KKLMREKGTSRHDLGRE 359

[Close]

BLAST of CK148894 vs. SwissProt
Match: SYV_BDEBA (Valyl-tRNA synthetase OS=Bdellovibrio bacteriovorus GN=valS PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 6.391e-21
Identity = 35/64 (54.69%), Postives = 41/64 (64.06%), Query Frame = 3

Query:  396 NPTAAEKSWYEWWEKSKYFGRC*QLQTT---FCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            NP   E   YEWWEK+ YF    Q Q+T   F   LPPPNVTG LH+GHAL   I+D MIRW++
Sbjct:   12 NPADVESRTYEWWEKNGYFKA--QDQSTKPPFSIILPPPNVTGFLHMGHALDHTIQDMMIRWKR 73

HSP 2 Score: 51.6026 bits (122), Expect = 6.391e-21
Identity = 24/47 (51.06%), Postives = 34/47 (72.34%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQLTNMIWAGK-ICKVW 715
            M+GYNT+W+PG DHAGIATQ VVE++L ++    + +   K + KVW
Sbjct:   74 MNGYNTMWLPGTDHAGIATQSVVERELKKDGVTRHDLGREKFVEKVW 120

[Close]

BLAST of CK148894 vs. SwissProt
Match: SYVM_PIG (Valyl-tRNA synthetase, mitochondrial OS=Sus scrofa GN=VARS2 PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 8.287e-21
Identity = 31/67 (46.27%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  396 NPTAAEKSWYEWWEKSKYFGRC*QLQT------TFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            +P   E +WY WW +  +F    Q +       TF  C+PPPNVTG+LHIGHALT AI+D ++RW +
Sbjct:  105 SPRYVEAAWYPWWVREGFFKPEYQTRLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHR 171

HSP 2 Score: 48.9062 bits (115), Expect = 8.287e-21
Identity = 23/33 (69.70%), Postives = 24/33 (72.73%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQL 676
            M G   LWVPG DHAGIATQ VVEKKL +E  L
Sbjct:  172 MRGDRVLWVPGSDHAGIATQAVVEKKLWKERGL 204

[Close]

BLAST of CK148894 vs. SwissProt
Match: SYVC_HUMAN (Valyl-tRNA synthetase OS=Homo sapiens GN=VARS PE=1 SV=4)

HSP 1 Score: 69.3218 bits (168), Expect = 1.075e-20
Identity = 31/69 (44.93%), Postives = 43/69 (62.32%), Query Frame = 3

Query:  396 NPTAAEKSWYEWWEKSKYF----GR----C*QLQTTFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            +P   E +WY WWE+  +F    GR        +  F  C+PPPNVTG+LH+GHALT AI+D++ RW +
Sbjct:  301 SPRYVEAAWYPWWEQQGFFKPEYGRPNVSAANPRGVFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHR 369

HSP 2 Score: 51.6026 bits (122), Expect = 1.075e-20
Identity = 25/34 (73.53%), Postives = 26/34 (76.47%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQLT 679
            M G  TLW PG DHAGIATQVVVEKKL RE  L+
Sbjct:  370 MRGETTLWNPGCDHAGIATQVVVEKKLWREQGLS 403

[Close]

BLAST of CK148894 vs. SwissProt
Match: SYVC_MOUSE (Valyl-tRNA synthetase OS=Mus musculus GN=Vars PE=2 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.075e-20
Identity = 32/75 (42.67%), Postives = 47/75 (62.67%), Query Frame = 3

Query:  378 AIGETDNPTAAEKSWYEWWEKSKYF----GR----C*QLQTTFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            A+ ++ +P   E +WY WWE+  +F    GR        +  F  C+PPPNVTG+LH+GHALT AI+D++ RW +
Sbjct:  294 AMPDSYSPQYVEAAWYPWWERQGFFKPEYGRPSVSAPNPRGVFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHR 368

HSP 2 Score: 50.0618 bits (118), Expect = 1.075e-20
Identity = 24/33 (72.73%), Postives = 25/33 (75.76%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQL 676
            M G  TLW PG DHAGIATQVVVEKKL +E  L
Sbjct:  369 MRGETTLWNPGCDHAGIATQVVVEKKLWKERGL 401

[Close]

BLAST of CK148894 vs. SwissProt
Match: SYVM_RAT (Valyl-tRNA synthetase, mitochondrial OS=Rattus norvegicus GN=Vars2 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.078e-20
Identity = 31/67 (46.27%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  396 NPTAAEKSWYEWWEKSKYFGRC*QLQT------TFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            +P   E +WY+WW +  +F    Q +       TF  C+PPPNVTG+LHIGHALT AI+D  +RW +
Sbjct:  105 SPQYVEAAWYQWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDAFVRWHR 171

HSP 2 Score: 48.1358 bits (113), Expect = 1.078e-20
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQL 676
            M G   LW+PG DHAGIATQ VVEK+L RE ++
Sbjct:  172 MRGDRVLWIPGSDHAGIATQAVVEKQLWRERRV 204

[Close]

BLAST of CK148894 vs. SwissProt
Match: SYVM_MOUSE (Valyl-tRNA synthetase, mitochondrial OS=Mus musculus GN=Vars2 PE=2 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 1.402e-20
Identity = 31/67 (46.27%), Postives = 43/67 (64.18%), Query Frame = 3

Query:  396 NPTAAEKSWYEWWEKSKYFGRC*QLQT------TFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            +P   E +WY+WW +  +F    Q +       TF  C+PPPNVTG+LHIGHALT AI+D ++RW +
Sbjct:  105 SPQYVEAAWYQWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHR 171

HSP 2 Score: 46.9802 bits (110), Expect = 1.402e-20
Identity = 20/33 (60.61%), Postives = 25/33 (75.76%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQL 676
            M G   LW+PG DHAGIATQ VVEK+L +E ++
Sbjct:  172 MRGDRVLWIPGSDHAGIATQAVVEKQLWKEQRV 204

[Close]

BLAST of CK148894 vs. SwissProt
Match: SYVC_DICDI (Probable valyl-tRNA synthetase, cytoplasmic OS=Dictyostelium discoideum GN=valS1 PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.823e-20
Identity = 29/78 (37.18%), Postives = 49/78 (62.82%), Query Frame = 3

Query:  369 NVAAIGETDNPTAAEKSWYEWWEKSKYFGRC*QLQTT--------FCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            +V+++  + +PTA E  WY++W  + YF    Q++          F   +PPPNVTG+LH+GHALT +I+D ++R+ +
Sbjct:   99 DVSSLLSSYHPTAVESIWYDYWLDNGYFSPEKQMEIQPHVVKDKKFVIVIPPPNVTGSLHLGHALTNSIQDAVVRYHR 176

HSP 2 Score: 55.8398 bits (133), Expect = 1.823e-20
Identity = 29/48 (60.42%), Postives = 35/48 (72.92%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQLT--NMIWAGKICKVW 715
            M G   LWVPG DHAGIATQVVVEKK+ +EN++T  ++   G I KVW
Sbjct:  177 MKGEVCLWVPGTDHAGIATQVVVEKKIWKENKITRHDLGREGFIKKVW 224

[Close]

BLAST of CK148894 vs. SwissProt
Match: SYVC_RAT (Valyl-tRNA synthetase OS=Rattus norvegicus GN=Vars PE=2 SV=2)

HSP 1 Score: 69.707 bits (169), Expect = 2.364e-20
Identity = 31/69 (44.93%), Postives = 43/69 (62.32%), Query Frame = 3

Query:  396 NPTAAEKSWYEWWEKSKYF----GR----C*QLQTTFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            +P   E +WY WWE+  +F    GR        +  F  C+PPPNVTG+LH+GHALT AI+D++ RW +
Sbjct:  301 SPQYVEAAWYPWWERQGFFKPEYGRPSVSAPNPRGVFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHR 369

HSP 2 Score: 50.0618 bits (118), Expect = 2.364e-20
Identity = 24/33 (72.73%), Postives = 25/33 (75.76%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQL 676
            M G  TLW PG DHAGIATQVVVEKKL +E  L
Sbjct:  370 MRGETTLWNPGCDHAGIATQVVVEKKLWKERGL 402

[Close]

BLAST of CK148894 vs. TrEMBL
Match: B9FAQ6_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_12199 PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 9.901e-31
Identity = 42/62 (67.74%), Postives = 46/62 (74.19%), Query Frame = 3

Query:  396 NPTAAEKSWYEWWEKSKYFGR-C*QLQTTFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            +PTA EKSWY WWE S YFG      +  F   LPPPNVTGALHIGHALT AIED+MIRWR+
Sbjct:   97 SPTAVEKSWYSWWESSGYFGADAASSKPPFVIVLPPPNVTGALHIGHALTVAIEDSMIRWRR 158

HSP 2 Score: 67.3958 bits (163), Expect = 9.901e-31
Identity = 31/34 (91.18%), Postives = 32/34 (94.12%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQLT 679
            MSGYNTLWVPG+DHAGIATQVVVEKKLMRE  LT
Sbjct:  159 MSGYNTLWVPGVDHAGIATQVVVEKKLMRERNLT 192

[Close]

BLAST of CK148894 vs. TrEMBL
Match: Q851K9_ORYSJ (Os03g0694900 protein OS=Oryza sativa subsp. japonica GN=OSJNBb0021O11.3 PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 9.902e-31
Identity = 42/62 (67.74%), Postives = 46/62 (74.19%), Query Frame = 3

Query:  396 NPTAAEKSWYEWWEKSKYFGR-C*QLQTTFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            +PTA EKSWY WWE S YFG      +  F   LPPPNVTGALHIGHALT AIED+MIRWR+
Sbjct:   97 SPTAVEKSWYSWWESSGYFGADAASSKPPFVIVLPPPNVTGALHIGHALTVAIEDSMIRWRR 158

HSP 2 Score: 67.3958 bits (163), Expect = 9.902e-31
Identity = 31/34 (91.18%), Postives = 32/34 (94.12%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQLT 679
            MSGYNTLWVPG+DHAGIATQVVVEKKLMRE  LT
Sbjct:  159 MSGYNTLWVPGVDHAGIATQVVVEKKLMRERNLT 192

[Close]

BLAST of CK148894 vs. TrEMBL
Match: B9SWL0_RICCO (Valyl-tRNA synthetase, putative OS=Ricinus communis GN=RCOM_0771810 PE=3 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 3.707e-30
Identity = 41/69 (59.42%), Postives = 49/69 (71.01%), Query Frame = 3

Query:  375 AAIGETDNPTAAEKSWYEWWEKSKYF-GRC*QLQTTFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            A + +  +P+A EKSWY WWEKS YF       +  F    PPPNVTGALHIGHALTAA+EDT+IRWR+
Sbjct:   92 AQMAKQYSPSAVEKSWYAWWEKSGYFTAHAKSSKPPFTIVFPPPNVTGALHIGHALTAAVEDTIIRWRR 160

HSP 2 Score: 67.3958 bits (163), Expect = 3.707e-30
Identity = 31/34 (91.18%), Postives = 32/34 (94.12%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQLT 679
            MSGYNTLWVPG+DHAGIATQVVVEKKLMRE  LT
Sbjct:  161 MSGYNTLWVPGVDHAGIATQVVVEKKLMRERHLT 194

[Close]

BLAST of CK148894 vs. TrEMBL
Match: B8APY8_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_13120 PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.707e-30
Identity = 42/62 (67.74%), Postives = 46/62 (74.19%), Query Frame = 3

Query:  396 NPTAAEKSWYEWWEKSKYFGR-C*QLQTTFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            +PTA EKSWY WWE S YFG      +  F   LPPPNVTGALHIGHALT AIED+MIRWR+
Sbjct:   97 SPTAVEKSWYSWWESSGYFGADAASSKPPFVIVLPPPNVTGALHIGHALTVAIEDSMIRWRR 158

HSP 2 Score: 65.4698 bits (158), Expect = 3.707e-30
Identity = 30/34 (88.24%), Postives = 31/34 (91.18%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQLT 679
            MSGYN LWVPG+DHAGIATQVVVEKKLMRE  LT
Sbjct:  159 MSGYNALWVPGVDHAGIATQVVVEKKLMRERNLT 192

[Close]

BLAST of CK148894 vs. TrEMBL
Match: D7TMX5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_100.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00010632001 PE=4 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 3.061e-29
Identity = 39/62 (62.90%), Postives = 47/62 (75.81%), Query Frame = 3

Query:  396 NPTAAEKSWYEWWEKSKYF-GRC*QLQTTFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            +P+A E SWYEWWEKS +F       +  F   LPPPNVTGALHIGHALT+AI+DT+IRWR+
Sbjct:  140 SPSAVENSWYEWWEKSGFFVADSSSSKPPFVIVLPPPNVTGALHIGHALTSAIQDTIIRWRR 201

HSP 2 Score: 67.3958 bits (163), Expect = 3.061e-29
Identity = 31/34 (91.18%), Postives = 32/34 (94.12%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQLT 679
            MSGYN LWVPGMDHAGIATQVVVEKKLMRE +LT
Sbjct:  202 MSGYNALWVPGMDHAGIATQVVVEKKLMRERKLT 235

[Close]

BLAST of CK148894 vs. TrEMBL
Match: C5YHH1_SORBI (Putative uncharacterized protein Sb07g004420 OS=Sorghum bicolor GN=Sb07g004420 PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 5.192e-29
Identity = 40/62 (64.52%), Postives = 45/62 (72.58%), Query Frame = 3

Query:  396 NPTAAEKSWYEWWEKSKYFGRC*-QLQTTFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            +P+A E+SWY WWE S YFG      +  F   LPPPNVTGALHIGHALT AIED MIRWR+
Sbjct:  103 SPSAVERSWYAWWESSGYFGADPASTKPPFVIVLPPPNVTGALHIGHALTVAIEDAMIRWRR 164

HSP 2 Score: 65.855 bits (159), Expect = 5.192e-29
Identity = 30/34 (88.24%), Postives = 32/34 (94.12%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQLT 679
            MSGYN LWVPG+DHAGIATQVVVEKKLMRE +LT
Sbjct:  165 MSGYNALWVPGVDHAGIATQVVVEKKLMRERKLT 198

[Close]

BLAST of CK148894 vs. TrEMBL
Match: B9HG11_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_820138 PE=3 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 2.529e-28
Identity = 41/62 (66.13%), Postives = 46/62 (74.19%), Query Frame = 3

Query:  396 NPTAAEKSWYEWWEKSKYF-GRC*QLQTTFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            NPT  EKSWY WWEKS YF       +  F   LPPPNVTGALHIGHALTAAIEDT+IR+++
Sbjct:   90 NPTVVEKSWYAWWEKSGYFVADANSSKPPFAIVLPPPNVTGALHIGHALTAAIEDTIIRFKR 151

HSP 2 Score: 63.1586 bits (152), Expect = 2.529e-28
Identity = 28/34 (82.35%), Postives = 32/34 (94.12%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQLT 679
            MSG+N LWVPG+DHAGIATQVVVEKKLMR++ LT
Sbjct:  152 MSGFNALWVPGVDHAGIATQVVVEKKLMRDHHLT 185

[Close]

BLAST of CK148894 vs. TrEMBL
Match: Q7G2B1_ORYSJ (Valyl-tRNA synthetase OS=Oryza sativa subsp. japonica GN=LOC_Os10g36210 PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.292e-28
Identity = 39/62 (62.90%), Postives = 43/62 (69.35%), Query Frame = 3

Query:  396 NPTAAEKSWYEWWEKSKYF-GRC*QLQTTFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            NP A EKSWY WWE S+YF       +  F   LPPPNVTG LHIGHA+T AIED MIRWR+
Sbjct:   89 NPAAVEKSWYSWWESSQYFVADATSSKPPFVIILPPPNVTGVLHIGHAITVAIEDAMIRWRR 150

HSP 2 Score: 63.1586 bits (152), Expect = 3.292e-28
Identity = 28/34 (82.35%), Postives = 31/34 (91.18%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQLT 679
            MSGYN LWVPGMDHAGIATQVVVEK+LMR+  L+
Sbjct:  151 MSGYNALWVPGMDHAGIATQVVVEKRLMRDRNLS 184

[Close]

BLAST of CK148894 vs. TrEMBL
Match: B9G6I9_ORYSJ (Os10g0506200 protein OS=Oryza sativa subsp. japonica GN=Os10g0506200 PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.293e-28
Identity = 39/62 (62.90%), Postives = 43/62 (69.35%), Query Frame = 3

Query:  396 NPTAAEKSWYEWWEKSKYF-GRC*QLQTTFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            NP A EKSWY WWE S+YF       +  F   LPPPNVTG LHIGHA+T AIED MIRWR+
Sbjct:   89 NPAAVEKSWYSWWESSQYFVADATSSKPPFVIILPPPNVTGVLHIGHAITVAIEDAMIRWRR 150

HSP 2 Score: 63.1586 bits (152), Expect = 3.293e-28
Identity = 28/34 (82.35%), Postives = 31/34 (91.18%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQLT 679
            MSGYN LWVPGMDHAGIATQVVVEK+LMR+  L+
Sbjct:  151 MSGYNALWVPGMDHAGIATQVVVEKRLMRDRNLS 184

[Close]

BLAST of CK148894 vs. TrEMBL
Match: D7KBM5_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_471639 PE=4 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 4.283e-28
Identity = 40/62 (64.52%), Postives = 45/62 (72.58%), Query Frame = 3

Query:  396 NPTAAEKSWYEWWEKSKYF-GRC*QLQTTFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            +P A EKSWY WWEKS  F       +  F   LPPPNVTGALHIGHALTAAIEDT+IRW++
Sbjct:  141 SPAAVEKSWYAWWEKSDLFKADAKSSKKPFVIVLPPPNVTGALHIGHALTAAIEDTIIRWKR 202

HSP 2 Score: 63.929 bits (154), Expect = 4.283e-28
Identity = 29/34 (85.29%), Postives = 31/34 (91.18%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQLT 679
            MSGYN LWVPG+DHAGIATQVVVEKKLMRE  +T
Sbjct:  203 MSGYNALWVPGVDHAGIATQVVVEKKLMRERGMT 236

[Close]

BLAST of CK148894 vs. TAIR peptide
Match: AT1G14610.1 (| Symbols: TWN2, VALRS | valyl-tRNA synthetase / valine--tRNA ligase (VALRS) | chr1:5008502-5014486 REVERSE LENGTH=1108)

HSP 1 Score: 83.1889 bits (204), Expect = 6.818e-29
Identity = 38/62 (61.29%), Postives = 44/62 (70.97%), Query Frame = 3

Query:  396 NPTAAEKSWYEWWEKSKYF-GRC*QLQTTFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            +P   EKSWY WWEKS  F       +  F   LPPPNVTGALHIGHALT+AIEDT+IRW++
Sbjct:  141 SPATVEKSWYAWWEKSDLFKADAKSSKPPFVIVLPPPNVTGALHIGHALTSAIEDTIIRWKR 202

HSP 2 Score: 62.003 bits (149), Expect = 6.818e-29
Identity = 27/34 (79.41%), Postives = 31/34 (91.18%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRENQLT 679
            MSGYN LWVPG+DHAGIATQVVVEKK+MR+  +T
Sbjct:  203 MSGYNALWVPGVDHAGIATQVVVEKKIMRDRGMT 236

HSP 3 Score: 50.8322 bits (120), Expect = 8.698e-7
Identity = 25/56 (44.64%), Postives = 35/56 (62.50%), Query Frame = 1

Query:  328 DYIDPETPLGDKKQMSRQLAKQITQLPRRNRGMSGGRNQSIL-GDASSSKPPFVIV 492
            D++DPETPLG++K++S Q+AKQ +         +      +   DA SSKPPFVIV
Sbjct:  118 DFVDPETPLGERKRLSSQMAKQYSPATVEKSWYAWWEKSDLFKADAKSSKPPFVIV 173

[Close]

BLAST of CK148894 vs. TAIR peptide
Match: AT5G16715.1 (| Symbols: EMB2247 | ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases | chr5:5485353-5493229 FORWARD LENGTH=974)

HSP 1 Score: 56.9954 bits (136), Expect = 8.657e-17
Identity = 25/59 (42.37%), Postives = 38/59 (64.41%), Query Frame = 3

Query:  405 AAEKSWYEWWEKSKYFG-RC*QLQTTFCYCLPPPNVTGALHIGHALTAAIEDTMIRWRK 578
            ++E+  Y+WWE   YF     Q  + F   +PPPNVTG+LH+GHA+   +ED M+R+ +
Sbjct:   76 SSEEKIYKWWESQGYFKPNFDQGGSPFVIPMPPPNVTGSLHMGHAMFVTLEDIMVRYNR 134

HSP 2 Score: 47.3654 bits (111), Expect = 8.657e-17
Identity = 24/50 (48.00%), Postives = 30/50 (60.00%), Query Frame = 2

Query:  578 MSGYNTLWVPGMDHAGIATQVVVEKKLMRE---------NQLTNMIWAGK 700
            M+G  TLW+PG DHAGIATQ+VVEK L  E         ++ T  +W  K
Sbjct:  135 MNGRPTLWLPGTDHAGIATQLVVEKMLASEGIKRVDLGRDEFTKRVWEWK 184

The following BLAST results are available for this feature:

BLAST of CK148894 vs. SwissProt
BLAST of CK148894 vs. TrEMBL
BLAST of CK148894 vs. TAIR peptide

BLAST of CK148894 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
SYV_ARATH	6.744e-28	61.29	Valyl-tRNA synthetase OS=Arabidopsis thaliana GN=V...	[more]
SYVC_TAKRU	1.136e-22	44.16	Valyl-tRNA synthetase OS=Takifugu rubripes GN=vars...	[more]
SYV_BDEBA	6.391e-21	54.69	Valyl-tRNA synthetase OS=Bdellovibrio bacteriovoru...	[more]
SYVM_PIG	8.287e-21	46.27	Valyl-tRNA synthetase, mitochondrial OS=Sus scrofa...	[more]
SYVC_HUMAN	1.075e-20	44.93	Valyl-tRNA synthetase OS=Homo sapiens GN=VARS PE=1...	[more]
SYVC_MOUSE	1.075e-20	42.67	Valyl-tRNA synthetase OS=Mus musculus GN=Vars PE=2...	[more]
SYVM_RAT	1.078e-20	46.27	Valyl-tRNA synthetase, mitochondrial OS=Rattus nor...	[more]
SYVM_MOUSE	1.402e-20	46.27	Valyl-tRNA synthetase, mitochondrial OS=Mus muscul...	[more]
SYVC_DICDI	1.823e-20	37.18	Probable valyl-tRNA synthetase, cytoplasmic OS=Dic...	[more]
SYVC_RAT	2.364e-20	44.93	Valyl-tRNA synthetase OS=Rattus norvegicus GN=Vars...	[more]

BLAST of CK148894 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9FAQ6_ORYSJ	9.901e-31	67.74	Putative uncharacterized protein OS=Oryza sativa s...	[more]
Q851K9_ORYSJ	9.902e-31	67.74	Os03g0694900 protein OS=Oryza sativa subsp. japoni...	[more]
B9SWL0_RICCO	3.707e-30	59.42	Valyl-tRNA synthetase, putative OS=Ricinus communi...	[more]
B8APY8_ORYSI	3.707e-30	67.74	Putative uncharacterized protein OS=Oryza sativa s...	[more]
D7TMX5_VITVI	3.061e-29	62.90	Whole genome shotgun sequence of line PN40024, sca...	[more]
C5YHH1_SORBI	5.192e-29	64.52	Putative uncharacterized protein Sb07g004420 OS=So...	[more]
B9HG11_POPTR	2.529e-28	66.13	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q7G2B1_ORYSJ	3.292e-28	62.90	Valyl-tRNA synthetase OS=Oryza sativa subsp. japon...	[more]
B9G6I9_ORYSJ	3.293e-28	62.90	Os10g0506200 protein OS=Oryza sativa subsp. japoni...	[more]
D7KBM5_ARALY	4.283e-28	64.52	Putative uncharacterized protein OS=Arabidopsis ly...	[more]

BLAST of CK148894 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G14610.1	6.818e-29	61.29	\| Symbols: TWN2, VALRS \| valyl-tRNA synthetase / v...	[more]
AT5G16715.1	8.657e-17	42.37	\| Symbols: EMB2247 \| ATP binding;valine-tRNA ligas...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 59..73 score
None	No IPR available	SEG	seg	seg	coord: 20..62 score
None	No IPR available	PANTHER	PTHR11946	ISOLEUCYL, LEUCYL, TYROSYL, VALYL AND METHIONYL-TRNA SYNTHETASES	coord: 5..37 score: 4.2
None	No IPR available	SUPERFAMILY	52374	Nucleotidylyl transferase	coord: 5..38 score: 6.5
None	No IPR available	SEG	seg	seg	coord: 120..125 score
None	No IPR available	SEG	seg	seg	coord: 2..21 score
IPR001412	Aminoacyl-tRNA synthetase, class I, conserved site	PROSITE	PS00178	AA_TRNA_LIGASE_I	coord: 13..24 score
IPR002300	Aminoacyl-tRNA synthetase, class Ia	PFAM	PF00133	tRNA-synt_1	coord: 3..38 score: 5.8
IPR002303	Valyl-tRNA synthetase	PANTHER	PTHR11946:SF5	VALYL-TRNA SYNTHETASE	coord: 5..37 score: 4.2
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	no description	coord: 4..39 score: 1.6

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK148894 ID=CK148894; Name=CK148894; organism=Cicer arietinum; type=EST; length=718bp
CGATATGGGATTTAAGACGTGGCCCGGGAATATGCTGCCCTTTGACTGAG
GGCTCTAGATCTCTGCGTCTCGCCCTCCATGGCTCATACTGCTTTCTGTA
TGGTAATTTCCGATCCTAAGCAAGAGCAAACGCCCACCCGTCTCGCTACT
CCGAAGTACCCCGAAAAGCAAGAAGCAAAAAAAAGAAAAGGCTGTAGAGA
AGCAATTGAAGAAAGAGAAATTTCTTCAAAAGCAAGCTCAGCAACAACAA
GCGCAACAATCATCCAATGCCTCTAAAAAGAGTGAAAAAAAAACTGTGAA
GCGTGGCGCGGAGGATGACAATCCTGTGACTACATTGATCCTGAGACTCC
ATTGGGTGATAAGAAACAAATGTCGCGGCAATTGGCGAAACAGATAACCC
AACTGCCGCGGAGAAATCGTGGTATGAGTGGTGGGAGAAATCAAAGTATT
TTGGGCGATGCTAGCAGCTCCAAACCACCTTTTGTTATTGTTTGCCTCCA
CCAAATGTGACTGGTGCTCTCCATATTGGTCATGCTCTAACAGCTGCAAT
AGAGGATACAATGATTCGTTGGCGTAAATGTCTGGATACAATACTTTGTG
GGTGCCTGGGATGGATCATGCTGGAATTGCCACACAGGTAGTTGTGGAAA
AAAAGTTAATGCGTGAAAATCAACTAACCAACATGATTTGGGCCGGAAAA
ATTTGCAAGGTTTGGAGG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0000166	nucleotide binding
GO:0004812	aminoacyl-tRNA ligase activity
GO:0005524	ATP binding
GO:0004832	valine-tRNA ligase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation
GO:0006418	tRNA aminoacylation for protein translation
GO:0006438	valyl-tRNA aminoacylation

Vocabulary: **INTERPRO**
Term	Definition
IPR001412	aa-tRNA-synth_I_CS
IPR002300	aa-tRNA-synth_Ia
IPR002303	Valyl-tRNA_ligase
IPR014729	Rossmann-like_a/b/a_fold

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