GR395239

EST Overview


Name	GR395239
Unique Name	GR395239
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	678

Libraries


Library Name	Type
LIBEST_024780 ICC1882 PEG induced drought cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR395239 vs. SwissProt
Match: ILV5_PEA (Ketol-acid reductoisomerase, chloroplastic OS=Pisum sativum GN=PGAAIR PE=2 SV=1)

HSP 1 Score: 298.516 bits (763), Expect = 2.149e-80
Identity = 146/165 (88.48%), Postives = 155/165 (93.94%), Query Frame = 1

Query:    7 MGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQTFVEVDNGAPINRALISNFITGPVHGAIEVCAELRPTLDISVPPDADFVRPELRQSNN 501
            MGKIDQTRMWKVGERVRSTRPAGD GPL PFTAGVFVA+MMAQIE+LR+KGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQ  V VD+GAPIN+ LISNF++ PVHGAI+VCAELRPTLDISVP  ADFVRPELRQ +N
Sbjct:  417 MGKIDQTRMWKVGERVRSTRPAGDLGPLYPFTAGVFVAMMMAQIEVLRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAVDSGAPINQDLISNFVSDPVHGAIQVCAELRPTLDISVPAAADFVRPELRQCSN 581

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BLAST of GR395239 vs. SwissProt
Match: ILV5_ARATH (Ketol-acid reductoisomerase, chloroplastic OS=Arabidopsis thaliana GN=At3g58610 PE=2 SV=2)

HSP 1 Score: 294.664 bits (753), Expect = 3.103e-79
Identity = 145/165 (87.88%), Postives = 153/165 (92.73%), Query Frame = 1

Query:    7 MGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQTFVEVDNGAPINRALISNFITGPVHGAIEVCAELRPTLDISVPPDADFVRPELRQSNN 501
            MG IDQTRMWKVGERVR +RPAGD GPL PFTAGV+VALMMAQIEILR+KGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQ  V VD+GA INR LISNF + PVHGAIEVCA+LRPT+DISVP DADFVRPELRQS+N
Sbjct:  427 MGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591

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BLAST of GR395239 vs. SwissProt
Match: ILV5_ORYSJ (Ketol-acid reductoisomerase, chloroplastic OS=Oryza sativa subsp. japonica GN=Os05g0573700 PE=1 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.224e-77
Identity = 141/164 (85.98%), Postives = 152/164 (92.68%), Query Frame = 1

Query:    7 MGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQTFVEVDNGAPINRALISNFITGPVHGAIEVCAELRPTLDISVPPDADFVRPELRQSN 498
            MG IDQTRMWKVGE+VRSTRP  D GPL PFTAGV+VALMMAQIE+LR+KGHSYSEIINESVIESVDSLNPFMHARGV+FMVDNCSTTARLGSRKWAPRFDYILTQQ FV VD  APIN+ LISNF++ PVHGAIEVCAELRPT+DISVP +ADFVRPELRQS+
Sbjct:  415 MGNIDQTRMWKVGEKVRSTRPENDLGPLHPFTAGVYVALMMAQIEVLRKKGHSYSEIINESVIESVDSLNPFMHARGVAFMVDNCSTTARLGSRKWAPRFDYILTQQAFVTVDKDAPINQDLISNFMSDPVHGAIEVCAELRPTVDISVPANADFVRPELRQSS 578

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BLAST of GR395239 vs. SwissProt
Match: ILV5_SPIOL (Ketol-acid reductoisomerase, chloroplastic OS=Spinacia oleracea GN=AHRI PE=1 SV=1)

HSP 1 Score: 283.878 bits (725), Expect = 5.477e-76
Identity = 139/163 (85.28%), Postives = 151/163 (92.64%), Query Frame = 1

Query:    7 MGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQTFVEVDNGAPINRALISNFITGPVHGAIEVCAELRPTLDISVPPDADFVRPELRQS 495
            MGKIDQTRMWKVGE+VRS RPAGD GPL PFTAGV+VALMMAQIEILR+KGHSYSEIINESVIE+VDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIL+QQ  V VDNGAPIN+ LISNF++ PVH AI VCA+LRP++DISV  DADFVRPELRQ+
Sbjct:  433 MGKIDQTRMWKVGEKVRSVRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSEIINESVIEAVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILSQQALVAVDNGAPINQDLISNFLSDPVHEAIGVCAQLRPSVDISVTADADFVRPELRQA 595

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BLAST of GR395239 vs. SwissProt
Match: ILV5_NEUCR (Ketol-acid reductoisomerase, mitochondrial OS=Neurospora crassa GN=ilv-2 PE=3 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 7.287e-12
Identity = 43/106 (40.57%), Postives = 58/106 (54.72%), Query Frame = 1

Query:   73 GDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILT---QQTFVEVDNGAPINRALISN 381
            G++G L     G+F    +AQ E+LR +GHS SE  NE+V E+  SL P + A G+ +M D CSTTAR G+  W P+F   L       +  V NG    R+L  N
Sbjct:  264 GERGCLMGGIHGMF----LAQYEVLRERGHSPSEAFNETVEEATQSLYPLIGAHGMDWMFDACSTTARRGAIDWTPKFKDALKPVFNNLYDSVKNGDETKRSLEYN 365

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BLAST of GR395239 vs. SwissProt
Match: ILV5_YEAST (Ketol-acid reductoisomerase, mitochondrial OS=Saccharomyces cerevisiae GN=ILV5 PE=1 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 6.169e-11
Identity = 47/129 (36.43%), Postives = 64/129 (49.61%), Query Frame = 1

Query:    4 GMGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILT---QQTFVEVDNGAPINRALISN 381
            G G + QT      ER  ++   G++G L     G+F    +AQ ++LR  GHS SE  NE+V E+  SL P +   G+ +M D CSTTAR G+  W P F   L    Q  +    NG    R+L  N
Sbjct:  239 GSGYVYQTTF----EREVNSDLYGERGCLMGGIHGMF----LAQYDVLRENGHSPSEAFNETVEEATQSLYPLIGKYGMDYMYDACSTTARRGALDWYPIFKNALKPVFQDLYESTKNGTETKRSLEFN 359

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BLAST of GR395239 vs. SwissProt
Match: ILV5_SCHPO (Probable ketol-acid reductoisomerase, mitochondrial OS=Schizosaccharomyces pombe GN=ilv5 PE=1 SV=2)

HSP 1 Score: 65.855 bits (159), Expect = 2.344e-10
Identity = 39/106 (36.79%), Postives = 59/106 (55.66%), Query Frame = 1

Query:   73 GDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRF---DYILTQQTFVEVDNGAPINRALISN 381
            G++G L     G+F    +AQ ++LR +GHS +E  NE+V E+  SL P +   G+ +M   CSTTAR G+  W PRF   +  +  + +  V+NG    R+L  N
Sbjct:  265 GERGCLMGGINGLF----LAQYQVLRERGHSPAEAFNETVEEATQSLYPLIGKYGLDYMFAACSTTARRGAIDWTPRFLEANKKVLNELYDNVENGNEAKRSLEYN 366

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BLAST of GR395239 vs. TrEMBL
Match: A5AGN5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_005408 PE=4 SV=1)

HSP 1 Score: 305.449 bits (781), Expect = 2.595e-81
Identity = 148/165 (89.70%), Postives = 156/165 (94.55%), Query Frame = 1

Query:    7 MGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQTFVEVDNGAPINRALISNFITGPVHGAIEVCAELRPTLDISVPPDADFVRPELRQSNN 501
            MGKIDQTRMWKVGERVRS RPAGD GPLCPFTAGV+VALMMAQIEILR+KGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYI+TQQ  V VDNG PINR LISNF++ PVHGAIEVCA+LRPT+DISVP DADFVRPELRQS+N
Sbjct:  424 MGKIDQTRMWKVGERVRSVRPAGDLGPLCPFTAGVYVALMMAQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIITQQALVAVDNGVPINRDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 588

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BLAST of GR395239 vs. TrEMBL
Match: D2DKE6_SOYBN (Acetohydroxyacid isomeroreductase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 303.908 bits (777), Expect = 7.550e-81
Identity = 149/165 (90.30%), Postives = 157/165 (95.15%), Query Frame = 1

Query:    7 MGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQTFVEVDNGAPINRALISNFITGPVHGAIEVCAELRPTLDISVPPDADFVRPELRQSNN 501
            MGKIDQTRMWKVGERVRSTRPAGD GPL PFTAGV+VALMMAQIEILR+KGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQ  V VDNGAPIN+ LISNF++  VHGAIEVCA+LRPT+DISVPPDADFVRPELRQS+N
Sbjct:  423 MGKIDQTRMWKVGERVRSTRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAVDNGAPINQDLISNFLSDQVHGAIEVCAQLRPTVDISVPPDADFVRPELRQSSN 587

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BLAST of GR395239 vs. TrEMBL
Match: B9H2U7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_817858 PE=4 SV=1)

HSP 1 Score: 301.982 bits (772), Expect = 2.869e-80
Identity = 146/165 (88.48%), Postives = 156/165 (94.55%), Query Frame = 1

Query:    7 MGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQTFVEVDNGAPINRALISNFITGPVHGAIEVCAELRPTLDISVPPDADFVRPELRQSNN 501
            MGKIDQTRMWKVGERVR+TRPAGD GPL PFTAGV+VALMMAQIEILR+KGHSYSEIINES+IESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYI+TQQ  V VDNG PINR LISNF + PVHGA+EVCA+LRPT+DISVPPDADFVRPELRQS+N
Sbjct:  425 MGKIDQTRMWKVGERVRATRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYSEIINESLIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAVDNGTPINRDLISNFFSDPVHGAVEVCAQLRPTVDISVPPDADFVRPELRQSSN 589

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BLAST of GR395239 vs. TrEMBL
Match: B7FLH4_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 301.597 bits (771), Expect = 3.747e-80
Identity = 150/164 (91.46%), Postives = 155/164 (94.51%), Query Frame = 1

Query:    7 MGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQTFVEVDNGAPINRALISNFITGPVHGAIEVCAELRPTLDISVPPDADFVRPELRQSN 498
            MGKIDQTRMWKVGE VRSTRPAGD GPL PFTAGVFVALMMAQIEILR+KGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQ  V VDNGAPI++ LISNF++ PVHGAIEVCAELRPTLDISVP DADFVRPELRQSN
Sbjct:  218 MGKIDQTRMWKVGEVVRSTRPAGDLGPLYPFTAGVFVALMMAQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAVDNGAPIDQDLISNFMSDPVHGAIEVCAELRPTLDISVPADADFVRPELRQSN 381

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BLAST of GR395239 vs. TrEMBL
Match: Q9SM58_PEA (Acetohydroxy acid isomeroreductase OS=Pisum sativum GN=aair PE=4 SV=1)

HSP 1 Score: 298.516 bits (763), Expect = 3.172e-79
Identity = 146/165 (88.48%), Postives = 155/165 (93.94%), Query Frame = 1

Query:    7 MGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQTFVEVDNGAPINRALISNFITGPVHGAIEVCAELRPTLDISVPPDADFVRPELRQSNN 501
            MGKIDQTRMWKVGERVRSTRPAGD GPL PFTAGVFVA+MMAQIE+LR+KGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQ  V VD+GAPIN+ LISNF++ PVHGAI+VCAELRPTLDISVP  ADFVRPELRQ +N
Sbjct:  417 MGKIDQTRMWKVGERVRSTRPAGDLGPLYPFTAGVFVAMMMAQIEVLRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAVDSGAPINQDLISNFVSDPVHGAIQVCAELRPTLDISVPAAADFVRPELRQCSN 581

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BLAST of GR395239 vs. TrEMBL
Match: D6QSY0_CATRO (Ketol-acid reductoisomerase OS=Catharanthus roseus GN=KARI PE=2 SV=1)

HSP 1 Score: 298.13 bits (762), Expect = 4.143e-79
Identity = 145/164 (88.41%), Postives = 153/164 (93.29%), Query Frame = 1

Query:    7 MGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQTFVEVDNGAPINRALISNFITGPVHGAIEVCAELRPTLDISVPPDADFVRPELRQSN 498
            MGKIDQTRMWKVGERVRS RP GD GPLCPFTAGV+VALMMAQIEILR+KGHSYSEIINESVIESVDSLNPFMHARGV+FMVDNCSTTARLGSRKWAPRFDYILTQQ  V VDN  PINR LISNF++ PVHGAIEVCA+LRPT+DISVP DADFVRPELRQS+
Sbjct:  424 MGKIDQTRMWKVGERVRSVRPTGDLGPLCPFTAGVYVALMMAQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVAFMVDNCSTTARLGSRKWAPRFDYILTQQAMVAVDNKTPINRDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSS 587

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BLAST of GR395239 vs. TrEMBL
Match: B9SGJ6_RICCO (Ketol-acid reductoisomerase, chloroplast, putative OS=Ricinus communis GN=RCOM_0553440 PE=4 SV=1)

HSP 1 Score: 297.745 bits (761), Expect = 5.410e-79
Identity = 146/165 (88.48%), Postives = 154/165 (93.33%), Query Frame = 1

Query:    7 MGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQTFVEVDNGAPINRALISNFITGPVHGAIEVCAELRPTLDISVPPDADFVRPELRQSNN 501
            MGKIDQTRMWKVGERVRSTR AGD GPL PFTAGV+VALMMAQIEILR+KGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQ  V VDNG P+   LISNF++ PVHGAIEVCA+LRPT+DISVPPDADFVRPELRQS+N
Sbjct:  420 MGKIDQTRMWKVGERVRSTRAAGDLGPLDPFTAGVYVALMMAQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQAMVSVDNGTPVKCDLISNFMSDPVHGAIEVCAQLRPTVDISVPPDADFVRPELRQSSN 584

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BLAST of GR395239 vs. TrEMBL
Match: B9N3F9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_827835 PE=4 SV=1)

HSP 1 Score: 295.819 bits (756), Expect = 2.056e-78
Identity = 144/165 (87.27%), Postives = 153/165 (92.73%), Query Frame = 1

Query:    7 MGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQTFVEVDNGAPINRALISNFITGPVHGAIEVCAELRPTLDISVPPDADFVRPELRQSNN 501
            MGKIDQTRMWKVGERVR+ RPAGD GPL PFTAGV+VALMMAQIEILR+KGHSYSEIINES+IESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQ  V VDN  PINR LISNF + PVHGA+EVCA+LRPT+DISVP DADFVRPELRQS+N
Sbjct:  361 MGKIDQTRMWKVGERVRAARPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYSEIINESLIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAVDNSTPINRDLISNFFSDPVHGAVEVCAQLRPTVDISVPADADFVRPELRQSSN 525

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BLAST of GR395239 vs. TrEMBL
Match: B5LAT1_CAPAN (Putative ketol-acid reductoisomerase OS=Capsicum annuum PE=2 SV=1)

HSP 1 Score: 295.434 bits (755), Expect = 2.685e-78
Identity = 145/165 (87.88%), Postives = 152/165 (92.12%), Query Frame = 1

Query:    7 MGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQTFVEVDNGAPINRALISNFITGPVHGAIEVCAELRPTLDISVPPDADFVRPELRQSNN 501
            MGKID TRMWKVGERVR+TRPAGD GPL PFTAGV+VALMMAQIE+LR+KGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDY LTQQ  V VDN APINR LISNF+  PVHGAIEVCA+LRPT+DISVP DADFVRPELRQS N
Sbjct:  427 MGKIDGTRMWKVGERVRATRPAGDLGPLYPFTAGVYVALMMAQIEVLRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYNLTQQALVAVDNNAPINRDLISNFLADPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSGN 591

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BLAST of GR395239 vs. TrEMBL
Match: Q56WU3_ARATH (Ketol-acid reductoisomerase OS=Arabidopsis thaliana GN=At3g58610 PE=2 SV=1)

HSP 1 Score: 294.664 bits (753), Expect = 4.580e-78
Identity = 145/165 (87.88%), Postives = 153/165 (92.73%), Query Frame = 1

Query:    7 MGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQTFVEVDNGAPINRALISNFITGPVHGAIEVCAELRPTLDISVPPDADFVRPELRQSNN 501
            MG IDQTRMWKVGERVR +RPAGD GPL PFTAGV+VALMMAQIEILR+KGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQ  V VD+GA INR LISNF + PVHGAIEVCA+LRPT+DISVP DADFVRPELRQS+N
Sbjct:  180 MGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 344

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BLAST of GR395239 vs. TAIR peptide
Match: AT3G58610.3 (| Symbols: | ketol-acid reductoisomerase | chr3:21671561-21674639 FORWARD LENGTH=591)

HSP 1 Score: 294.664 bits (753), Expect = 3.142e-80
Identity = 145/165 (87.88%), Postives = 153/165 (92.73%), Query Frame = 1

Query:    7 MGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQTFVEVDNGAPINRALISNFITGPVHGAIEVCAELRPTLDISVPPDADFVRPELRQSNN 501
            MG IDQTRMWKVGERVR +RPAGD GPL PFTAGV+VALMMAQIEILR+KGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQ  V VD+GA INR LISNF + PVHGAIEVCA+LRPT+DISVP DADFVRPELRQS+N
Sbjct:  427 MGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591

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BLAST of GR395239 vs. TAIR peptide
Match: AT3G58610.2 (| Symbols: | ketol-acid reductoisomerase | chr3:21671561-21674639 FORWARD LENGTH=591)

HSP 1 Score: 294.664 bits (753), Expect = 3.142e-80
Identity = 145/165 (87.88%), Postives = 153/165 (92.73%), Query Frame = 1

Query:    7 MGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQTFVEVDNGAPINRALISNFITGPVHGAIEVCAELRPTLDISVPPDADFVRPELRQSNN 501
            MG IDQTRMWKVGERVR +RPAGD GPL PFTAGV+VALMMAQIEILR+KGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQ  V VD+GA INR LISNF + PVHGAIEVCA+LRPT+DISVP DADFVRPELRQS+N
Sbjct:  427 MGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591

[Close]

BLAST of GR395239 vs. TAIR peptide
Match: AT3G58610.1 (| Symbols: | ketol-acid reductoisomerase | chr3:21671561-21674639 FORWARD LENGTH=591)

HSP 1 Score: 294.664 bits (753), Expect = 3.142e-80
Identity = 145/165 (87.88%), Postives = 153/165 (92.73%), Query Frame = 1

Query:    7 MGKIDQTRMWKVGERVRSTRPAGDQGPLCPFTAGVFVALMMAQIEILRRKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQTFVEVDNGAPINRALISNFITGPVHGAIEVCAELRPTLDISVPPDADFVRPELRQSNN 501
            MG IDQTRMWKVGERVR +RPAGD GPL PFTAGV+VALMMAQIEILR+KGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQ  V VD+GA INR LISNF + PVHGAIEVCA+LRPT+DISVP DADFVRPELRQS+N
Sbjct:  427 MGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591

The following BLAST results are available for this feature:

BLAST of GR395239 vs. SwissProt
BLAST of GR395239 vs. TrEMBL
BLAST of GR395239 vs. TAIR peptide

BLAST of GR395239 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 7


Match Name	E-value	Identity	Description
ILV5_PEA	2.149e-80	88.48	Ketol-acid reductoisomerase, chloroplastic OS=Pisu...	[more]
ILV5_ARATH	3.103e-79	87.88	Ketol-acid reductoisomerase, chloroplastic OS=Arab...	[more]
ILV5_ORYSJ	2.224e-77	85.98	Ketol-acid reductoisomerase, chloroplastic OS=Oryz...	[more]
ILV5_SPIOL	5.477e-76	85.28	Ketol-acid reductoisomerase, chloroplastic OS=Spin...	[more]
ILV5_NEUCR	7.287e-12	40.57	Ketol-acid reductoisomerase, mitochondrial OS=Neur...	[more]
ILV5_YEAST	6.169e-11	36.43	Ketol-acid reductoisomerase, mitochondrial OS=Sacc...	[more]
ILV5_SCHPO	2.344e-10	36.79	Probable ketol-acid reductoisomerase, mitochondria...	[more]

BLAST of GR395239 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A5AGN5_VITVI	2.595e-81	89.70	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D2DKE6_SOYBN	7.550e-81	90.30	Acetohydroxyacid isomeroreductase OS=Glycine max P...	[more]
B9H2U7_POPTR	2.869e-80	88.48	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B7FLH4_MEDTR	3.747e-80	91.46	Putative uncharacterized protein OS=Medicago trunc...	[more]
Q9SM58_PEA	3.172e-79	88.48	Acetohydroxy acid isomeroreductase OS=Pisum sativu...	[more]
D6QSY0_CATRO	4.143e-79	88.41	Ketol-acid reductoisomerase OS=Catharanthus roseus...	[more]
B9SGJ6_RICCO	5.410e-79	88.48	Ketol-acid reductoisomerase, chloroplast, putative...	[more]
B9N3F9_POPTR	2.056e-78	87.27	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B5LAT1_CAPAN	2.685e-78	87.88	Putative ketol-acid reductoisomerase OS=Capsicum a...	[more]
Q56WU3_ARATH	4.580e-78	87.88	Ketol-acid reductoisomerase OS=Arabidopsis thalian...	[more]

BLAST of GR395239 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT3G58610.3	3.142e-80	87.88	\| Symbols: \| ketol-acid reductoisomerase \| chr3:2...	[more]
AT3G58610.2	3.142e-80	87.88	\| Symbols: \| ketol-acid reductoisomerase \| chr3:2...	[more]
AT3G58610.1	3.142e-80	87.88	\| Symbols: \| ketol-acid reductoisomerase \| chr3:2...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000506	Acetohydroxy acid isomeroreductase C-terminal	PFAM	PF01450	IlvC	coord: 25..162 score: 2.9
IPR008927	6-phosphogluconate dehydrogenase, C-terminal-like	SUPERFAMILY	48179	6-phosphogluconate dehydrogenase C-terminal domain-like	coord: 3..165 score: 2.7
IPR013023	Acetohydroxy acid isomeroreductase	PANTHER	PTHR21371	FAMILY NOT NAMED	coord: 3..36 score: 6.1
IPR013328	Dehydrogenase, multihelical	GENE3D	1.10.1040.10	no description	coord: 26..132 score: 1.3
None	No IPR available	PANTHER	PTHR21371:SF4	gb def: Ketol-acid reductoisomerase, chloroplast precursor (EC 1.1.1.86) (Acetohydroxy-a	coord: 3..36 score: 6.1
None	No IPR available	SEG	seg	seg	coord: 38..51 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR395239 ID=GR395239; Name=GR395239; organism=Cicer arietinum; type=EST; length=678bp
GCGGGCATGGGTAAAATTGACCAGACTCGCATGTGGAAGGTTGGCGAGCG
TGTTCGATCTACAAGACCAGCAGGTGATCAAGGCCCATTATGTCCTTTTA
CTGCTGGTGTCTTTGTAGCATTGATGATGGCCCAGATTGAGATCCTGAGA
AGGAAAGGTCATTCTTACTCTGAAATCATTAACGAGAGTGTTATTGAGTC
TGTTGATTCTTTGAATCCTTTCATGCATGCTCGTGGTGTTTCCTTCATGG
TTGATAACTGTTCAACCACGGCTAGGTTGGGCTCGAGGAAATGGGCTCCA
CGATTTGATTACATCCTTACCCAGCAGACCTTTGTGGAAGTGGACAATGG
TGCGCCTATTAACCGGGCCCTAATCAGCAACTTCATAACAGGCCCTGTTC
ATGGTGCCATTGAAGTTTGTGCTGAACTGAGACCTACATTAGACATTTCT
GTGCCACCTGATGCAGACTTTGTTCGTCCCGAGTTGCGTCAATCCAACAA
TTAGAACATTAATCAATTATGGCTTTTATATTATGTATAGTTTGTTCAAA
ATAGTTGTTGTATTAGTCCTGAATTTTCAGTTGTTTCTTAATGTTATGTG
AACTAGGAATGCTACTTGACATCCTATTCAATATTAATGTAAGTTAATAA
AGCATGTCATGCAAGTGTAAGGTGGCTC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004455	ketol-acid reductoisomerase activity
GO:0016491	oxidoreductase activity
GO:0050662	obsolete coenzyme binding

Vocabulary: **Biological Process**
Term	Definition
GO:0009082	branched-chain amino acid biosynthetic process
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR000506	KARI_C
IPR008927	6-PGluconate_DH-like_C_sf
IPR013023	KARI
IPR013328	6PGD_dom2

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