GR408812

EST Overview


Name	GR408812
Unique Name	GR408812
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	611

Libraries


Library Name	Type
LIBEST_024786 JG11 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR408812 vs. SwissProt
Match: RL27_PEA (60S ribosomal protein L27 OS=Pisum sativum GN=RPL27 PE=2 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 1.780e-55
Identity = 96/115 (83.48%), Postives = 100/115 (86.96%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            KKAVIV+TFDDGTREKPYGHCLVAGIKK+PSKVI+KDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAV  DVLQ+KDKKVTAL             GKNRWFFTKLRF
Sbjct:   21 KKAVIVKTFDDGTREKPYGHCLVAGIKKFPSKVIKKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVVPDVLQSKDKKVTALKETKKSLEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 45.8246 bits (107), Expect = 1.780e-55
Identity = 21/22 (95.45%), Postives = 22/22 (100.00%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            MVKFLKPNKAVI+LQGRYAGKK
Sbjct:    1 MVKFLKPNKAVILLQGRYAGKK 22

[Close]

BLAST of GR408812 vs. SwissProt
Match: RL273_ARATH (60S ribosomal protein L27-3 OS=Arabidopsis thaliana GN=RPL27C PE=2 SV=2)

HSP 1 Score: 178.333 bits (451), Expect = 1.220e-50
Identity = 84/115 (73.04%), Postives = 96/115 (83.48%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            KKAVI+++FDDG R++PYGHCLVAG+KKYPSKVI+KDSAKKTAKKSRVK F+KLVNYQHLMPTRYTLDVDLK+  ++D LQ+KDKKV AL             GKNRWFFTKLRF
Sbjct:   21 KKAVIIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAALKEAKAKLEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 42.743 bits (99), Expect = 1.220e-50
Identity = 20/22 (90.91%), Postives = 21/22 (95.45%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            MVKFLK NKAVI+LQGRYAGKK
Sbjct:    1 MVKFLKQNKAVILLQGRYAGKK 22

[Close]

BLAST of GR408812 vs. SwissProt
Match: RL272_ARATH (60S ribosomal protein L27-2 OS=Arabidopsis thaliana GN=RPL27B PE=2 SV=2)

HSP 1 Score: 174.866 bits (442), Expect = 1.325e-49
Identity = 83/115 (72.17%), Postives = 96/115 (83.48%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            KKAVI+++FDDGT ++ YGHCLVAG+KKYPSKVI+KDSAKKTAKKSRVK F+KLVNYQHLMPTRYTLDVDLK+  ++D L++KDKKVTAL             GKNRWFFTKLRF
Sbjct:   21 KKAVIIKSFDDGTSDRRYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALKSKDKKVTALKEAKAKLEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 42.743 bits (99), Expect = 1.325e-49
Identity = 20/22 (90.91%), Postives = 21/22 (95.45%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            MVKFLK NKAVI+LQGRYAGKK
Sbjct:    1 MVKFLKQNKAVILLQGRYAGKK 22

[Close]

BLAST of GR408812 vs. SwissProt
Match: RL271_ARATH (60S ribosomal protein L27-1 OS=Arabidopsis thaliana GN=RPL27A PE=2 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 1.562e-47
Identity = 83/115 (72.17%), Postives = 94/115 (81.74%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            KKAVIV++FDDGT EK YGHCLVAG+KKYPSKVI+KDSAKKTAKKSRVK F K++NYQH+MPTRYTLD+DLK+ VS D + +KDKKVTAL             GKNRWFFTKLRF
Sbjct:   21 KKAVIVKSFDDGTVEKKYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFFKVINYQHVMPTRYTLDLDLKNVVSADAISSKDKKVTALKEAKAKFEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 38.1206 bits (87), Expect = 1.562e-47
Identity = 17/22 (77.27%), Postives = 19/22 (86.36%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            MVK +KP KAVI+LQGRY GKK
Sbjct:    1 MVKCMKPGKAVILLQGRYTGKK 22

[Close]

BLAST of GR408812 vs. SwissProt
Match: RL27_SOLTU (60S ribosomal protein L27 OS=Solanum tuberosum GN=RPL27 PE=2 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 2.394e-45
Identity = 82/119 (68.91%), Postives = 92/119 (77.31%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDL----KDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            +KAVIVR FD+GTR++PYGHCLVAGI +YP KVI+KDSAKK  KKSRVKAF+KLVNY H+MPTRYTLD DL    KD V+ DVLQA+DKKVTA              GKNRWFFTKLRF
Sbjct:   21 RKAVIVRAFDEGTRDRPYGHCLVAGISRYPKKVIRKDSAKKAGKKSRVKAFIKLVNYNHIMPTRYTLDEDLKDVVKDVVNADVLQARDKKVTA-RRTKARLAERFKTGKNRWFFTKLRF 138

HSP 2 Score: 41.2022 bits (95), Expect = 2.394e-45
Identity = 18/22 (81.82%), Postives = 21/22 (95.45%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            M KFLKPNKAVI+LQG+YAG+K
Sbjct:    1 MEKFLKPNKAVILLQGKYAGRK 22

[Close]

BLAST of GR408812 vs. SwissProt
Match: RL27_PYRST (60S ribosomal protein L27 OS=Pyrobotrys stellata GN=RPL27 PE=2 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 3.362e-34
Identity = 66/115 (57.39%), Postives = 76/115 (66.09%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            KKAVIVR FDDGT  +PYGH LV G++K P KV ++ S KK AKKS +K F+K VNY HLMPTRYTLDVD K  V+ +  +   KKV A              GKNRWFFTKLRF
Sbjct:   21 KKAVIVRNFDDGTSSRPYGHALVVGLQKEPRKVTKRQSQKKQAKKSTLKTFIKTVNYNHLMPTRYTLDVDFK-GVAAEAQENPTKKVEARKECKKLLEEKFKTGKNRWFFTKLRF 134

HSP 2 Score: 36.1946 bits (82), Expect = 3.362e-34
Identity = 16/22 (72.73%), Postives = 18/22 (81.82%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            MVKFLK  K V+VL GR+AGKK
Sbjct:    1 MVKFLKSGKVVVVLSGRFAGKK 22

[Close]

BLAST of GR408812 vs. SwissProt
Match: RL27B_YEAST (60S ribosomal protein L27-B OS=Saccharomyces cerevisiae GN=RPL27B PE=1 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 2.052e-30
Identity = 56/116 (48.28%), Postives = 78/116 (67.24%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVD-LKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            KK VIV+  D+G++  P+GH LVAGI++YPSKV +K  AKK AK++++K F+K+VNY HL+PTRYTLDV+  K  VS +  +   ++  A              GKN+WFF+KLRF
Sbjct:   21 KKVVIVKPHDEGSKSHPFGHALVAGIERYPSKVTKKHGAKKVAKRTKIKPFIKVVNYNHLLPTRYTLDVEAFKSVVSTETFEQPSQREEAKKVVKKAFEERHQAGKNQWFFSKLRF 136

HSP 2 Score: 33.8834 bits (76), Expect = 2.052e-30
Identity = 14/22 (63.64%), Postives = 17/22 (77.27%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            M KFLK  K  +V++GRYAGKK
Sbjct:    1 MAKFLKAGKVAVVVRGRYAGKK 22

[Close]

BLAST of GR408812 vs. SwissProt
Match: RL27A_YEAST (60S ribosomal protein L27-A OS=Saccharomyces cerevisiae GN=RPL27A PE=1 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 1.000e-29
Identity = 55/116 (47.41%), Postives = 77/116 (66.38%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVD-LKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            KK VIV+  D+G++  P+GH LVAGI++YP KV +K  AKK AK++++K F+K+VNY HL+PTRYTLDV+  K  VS +  +   ++  A              GKN+WFF+KLRF
Sbjct:   21 KKVVIVKPHDEGSKSHPFGHALVAGIERYPLKVTKKHGAKKVAKRTKIKPFIKVVNYNHLLPTRYTLDVEAFKSVVSTETFEQPSQREEAKKVVKKAFEERHQAGKNQWFFSKLRF 136

HSP 2 Score: 33.8834 bits (76), Expect = 1.000e-29
Identity = 14/22 (63.64%), Postives = 17/22 (77.27%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            M KFLK  K  +V++GRYAGKK
Sbjct:    1 MAKFLKAGKVAVVVRGRYAGKK 22

[Close]

BLAST of GR408812 vs. SwissProt
Match: RL27_HIPCM (60S ribosomal protein L27 OS=Hippocampus comes GN=rpl27 PE=2 SV=2)

HSP 1 Score: 113.62 bits (283), Expect = 4.875e-29
Identity = 58/116 (50.00%), Postives = 73/116 (62.93%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDL-KDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            +KAVIV+  DDGT ++PY H LVAGI +YP KV      KK AK+S++KAFVK+ NY HLMPTRY++D+ L K  V+ DV +    K  A              GKN+WFF KLRF
Sbjct:   21 RKAVIVKNIDDGTADRPYSHALVAGIDRYPRKVTAPMGKKKIAKRSKIKAFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKSKARREAKVKFEERYKTGKNKWFFQKLRF 136

HSP 2 Score: 35.039 bits (79), Expect = 4.875e-29
Identity = 15/22 (68.18%), Postives = 18/22 (81.82%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            M KF+KP K V+VL GRYAG+K
Sbjct:    1 MGKFMKPGKVVMVLAGRYAGRK 22

[Close]

BLAST of GR408812 vs. SwissProt
Match: RL27_DANRE (60S ribosomal protein L27 OS=Danio rerio GN=rpl27 PE=2 SV=3)

HSP 1 Score: 113.235 bits (282), Expect = 6.348e-29
Identity = 58/116 (50.00%), Postives = 73/116 (62.93%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDL-KDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            +KAVIV+  DDGT ++PY H LVAGI +YP KV      KK AK+S++KAFVK+ NY HLMPTRY++D+ L K  V+ DV +    K  A              GKN+WFF KLRF
Sbjct:   21 RKAVIVKNIDDGTADRPYSHALVAGIDRYPRKVTATMGKKKIAKRSKIKAFVKVFNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136

HSP 2 Score: 35.039 bits (79), Expect = 6.348e-29
Identity = 15/22 (68.18%), Postives = 18/22 (81.82%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            M KF+KP K V+VL GRYAG+K
Sbjct:    1 MGKFMKPGKVVMVLAGRYAGRK 22

[Close]

BLAST of GR408812 vs. TrEMBL
Match: C6TMU7_SOYBN (60S ribosomal protein L27 OS=Glycine max PE=2 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 1.979e-54
Identity = 95/115 (82.61%), Postives = 100/115 (86.96%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            KKAVIVRTFD+GTRE+PYGHCLVAGIKKYP+KVI+KDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAV+ DVL AKDKKVTAL             GKNRWFFTKLRF
Sbjct:   21 KKAVIVRTFDEGTRERPYGHCLVAGIKKYPAKVIKKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVNPDVLNAKDKKVTALKETKKRLEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 46.9802 bits (110), Expect = 1.979e-54
Identity = 22/22 (100.00%), Postives = 22/22 (100.00%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            MVKFLKPNKAVIVLQGRYAGKK
Sbjct:    1 MVKFLKPNKAVIVLQGRYAGKK 22

[Close]

BLAST of GR408812 vs. TrEMBL
Match: C6T3R4_SOYBN (60S ribosomal protein L27 OS=Glycine max PE=2 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 1.651e-53
Identity = 94/115 (81.74%), Postives = 99/115 (86.09%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            +KAVIVRTFD+GTRE+PYGHCLVAGIKKYPSKVI+KDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAV+ DVL  KDKKVTAL             GKNRWFFTKLRF
Sbjct:   21 RKAVIVRTFDEGTRERPYGHCLVAGIKKYPSKVIKKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVTPDVLATKDKKVTALKETKKRLEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 45.8246 bits (107), Expect = 1.651e-53
Identity = 21/22 (95.45%), Postives = 22/22 (100.00%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            MVKFLKPNKAVIVLQGRYAG+K
Sbjct:    1 MVKFLKPNKAVIVLQGRYAGRK 22

[Close]

BLAST of GR408812 vs. TrEMBL
Match: B7FH51_MEDTR (60S ribosomal protein L27 OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 184.496 bits (467), Expect = 1.378e-52
Identity = 91/115 (79.13%), Postives = 98/115 (85.22%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            KKAVIV+ FDDGTRE+ YGHCLVAGIKKYP+KVI+KDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLK+ V+ DVLQ+KDKKVTAL             GKNRWFFTKLRF
Sbjct:   21 KKAVIVKNFDDGTRERAYGHCLVAGIKKYPAKVIKKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKEVVTTDVLQSKDKKVTALKEAKKRFEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 46.9802 bits (110), Expect = 1.378e-52
Identity = 22/22 (100.00%), Postives = 22/22 (100.00%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            MVKFLKPNKAVIVLQGRYAGKK
Sbjct:    1 MVKFLKPNKAVIVLQGRYAGKK 22

[Close]

BLAST of GR408812 vs. TrEMBL
Match: B7FN57_MEDTR (60S ribosomal protein L27 OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 8.816e-52
Identity = 89/115 (77.39%), Postives = 98/115 (85.22%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            KKAVIV+ FDDGTR++ YGHCLVAGIKKYP+KVI+KDSAKKTAKKSRV+AFVKLVNYQHLMPTRYTLDVDLK+ V+ DVLQ+KDKKVTAL             GKNRWFFTKLRF
Sbjct:   21 KKAVIVKNFDDGTRDRAYGHCLVAGIKKYPAKVIKKDSAKKTAKKSRVEAFVKLVNYQHLMPTRYTLDVDLKEVVTTDVLQSKDKKVTALKEAKKRFEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 46.9802 bits (110), Expect = 8.816e-52
Identity = 22/22 (100.00%), Postives = 22/22 (100.00%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            MVKFLKPNKAVIVLQGRYAGKK
Sbjct:    1 MVKFLKPNKAVIVLQGRYAGKK 22

[Close]

BLAST of GR408812 vs. TrEMBL
Match: A9PBR8_POPTR (60S ribosomal protein L27 OS=Populus trichocarpa GN=POPTRDRAFT_824991 PE=2 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 2.768e-50
Identity = 87/115 (75.65%), Postives = 94/115 (81.74%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            +KAVIV+ FDDGTR++PYGHCLVAGIKKYPSKVI+KDS KKTAKKSRVK F+KLVNYQHLMPTRYTLDVDLKDAV+ D L  KDKKVTA              GKNRWFFTKLRF
Sbjct:   21 RKAVIVKHFDDGTRDRPYGHCLVAGIKKYPSKVIKKDSTKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKDAVTADSLTTKDKKVTACKDTKARFEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 45.4394 bits (106), Expect = 2.768e-50
Identity = 20/22 (90.91%), Postives = 22/22 (100.00%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            MVKFLKPNKAVI+LQGRYAG+K
Sbjct:    1 MVKFLKPNKAVIILQGRYAGRK 22

[Close]

BLAST of GR408812 vs. TrEMBL
Match: A9PAT1_POPTR (60S ribosomal protein L27 OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 4.703e-50
Identity = 86/115 (74.78%), Postives = 94/115 (81.74%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            +K VIVR+FDDGTR++PYGHCLVAGIKKYPSKVI+KDSAKKTAKKSRVK F+KLVNYQHLMPTRYTLDVDLKD V+ D L  KDKK+TA              GKNRWFFTKLRF
Sbjct:   21 RKGVIVRSFDDGTRDRPYGHCLVAGIKKYPSKVIKKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKDVVTADYLSTKDKKITACKETKARFEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 43.5134 bits (101), Expect = 4.703e-50
Identity = 19/23 (82.61%), Postives = 22/23 (95.65%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKKG 152
            MVKFLK NKAVI+LQG+YAG+KG
Sbjct:    1 MVKFLKTNKAVIILQGKYAGRKG 23

[Close]

BLAST of GR408812 vs. TrEMBL
Match: A9P8D7_POPTR (60S ribosomal protein L27 OS=Populus trichocarpa GN=POPTRDRAFT_706822 PE=2 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 4.703e-50
Identity = 86/115 (74.78%), Postives = 94/115 (81.74%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            +K VIVR+FDDGTR++PYGHCLVAGIKKYPSKVI+KDSAKKTAKKSRVK F+KLVNYQHLMPTRYTLDVDLKD V+ D L  KDKK+TA              GKNRWFFTKLRF
Sbjct:   21 RKGVIVRSFDDGTRDRPYGHCLVAGIKKYPSKVIKKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKDVVTADCLSTKDKKITACKETKARFEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 43.5134 bits (101), Expect = 4.703e-50
Identity = 19/23 (82.61%), Postives = 22/23 (95.65%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKKG 152
            MVKFLK NKAVI+LQG+YAG+KG
Sbjct:    1 MVKFLKTNKAVIILQGKYAGRKG 23

[Close]

BLAST of GR408812 vs. TrEMBL
Match: Q0WRB8_ARATH (60S ribosomal protein L27 OS=Arabidopsis thaliana GN=At4g15000 PE=2 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 1.770e-49
Identity = 84/115 (73.04%), Postives = 96/115 (83.48%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            KKAVI+++FDDG R++PYGHCLVAG+KKYPSKVI+KDSAKKTAKKSRVK F+KLVNYQHLMPTRYTLDVDLK+  ++D LQ+KDKKV AL             GKNRWFFTKLRF
Sbjct:   21 KKAVIIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAALKEAKAKLEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 42.743 bits (99), Expect = 1.770e-49
Identity = 20/22 (90.91%), Postives = 21/22 (95.45%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            MVKFLK NKAVI+LQGRYAGKK
Sbjct:    1 MVKFLKQNKAVILLQGRYAGKK 22

[Close]

BLAST of GR408812 vs. TrEMBL
Match: B9HD67_POPTR (60S ribosomal protein L27 OS=Populus trichocarpa GN=POPTRDRAFT_560241 PE=3 SV=1)

HSP 1 Score: 174.866 bits (442), Expect = 2.307e-49
Identity = 86/115 (74.78%), Postives = 93/115 (80.87%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            +KAVIV+ FDDGTR++PYGHCLVAGIKKYPSKVI+KDSAKKTAKKSRVK FVK+VNYQHLMPTRYTLDVDLKD V+ D L  KDKKV A              GKNRWFFTKLRF
Sbjct:   21 RKAVIVKHFDDGTRDRPYGHCLVAGIKKYPSKVIKKDSAKKTAKKSRVKCFVKVVNYQHLMPTRYTLDVDLKDLVTPDCLATKDKKVVACKETKTRFEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 45.8246 bits (107), Expect = 2.307e-49
Identity = 21/22 (95.45%), Postives = 22/22 (100.00%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            MVKFLKPNKAVIVLQGRYAG+K
Sbjct:    1 MVKFLKPNKAVIVLQGRYAGRK 22

[Close]

BLAST of GR408812 vs. TrEMBL
Match: D7MB93_ARALY (60S ribosomal protein L27 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_493389 PE=4 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 3.921e-49
Identity = 84/115 (73.04%), Postives = 96/115 (83.48%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            KKAVI+++FDDGTR++ YGHCLVAG+KKYPSKVI+KDSAKKTAKKSRVK F+KLVNYQHLMPTRYTLDVDLK+  ++D LQ+KDKKV AL             GKNRWFFTKLRF
Sbjct:   21 KKAVIIKSFDDGTRDRAYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAALKEAKAKLEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 42.743 bits (99), Expect = 3.921e-49
Identity = 20/22 (90.91%), Postives = 21/22 (95.45%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            MVKFLK NKAVI+LQGRYAGKK
Sbjct:    1 MVKFLKQNKAVILLQGRYAGKK 22

[Close]

BLAST of GR408812 vs. TAIR peptide
Match: AT4G15000.1 (| Symbols: | Ribosomal L27e protein family | chr4:8571896-8572303 FORWARD LENGTH=135)

HSP 1 Score: 178.333 bits (451), Expect = 1.280e-51
Identity = 84/115 (73.04%), Postives = 96/115 (83.48%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            KKAVI+++FDDG R++PYGHCLVAG+KKYPSKVI+KDSAKKTAKKSRVK F+KLVNYQHLMPTRYTLDVDLK+  ++D LQ+KDKKV AL             GKNRWFFTKLRF
Sbjct:   21 KKAVIIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAALKEAKAKLEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 42.743 bits (99), Expect = 1.280e-51
Identity = 20/22 (90.91%), Postives = 21/22 (95.45%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            MVKFLK NKAVI+LQGRYAGKK
Sbjct:    1 MVKFLKQNKAVILLQGRYAGKK 22

[Close]

BLAST of GR408812 vs. TAIR peptide
Match: AT3G22230.1 (| Symbols: | Ribosomal L27e protein family | chr3:7844136-7844543 REVERSE LENGTH=135)

HSP 1 Score: 174.866 bits (442), Expect = 1.391e-50
Identity = 83/115 (72.17%), Postives = 96/115 (83.48%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            KKAVI+++FDDGT ++ YGHCLVAG+KKYPSKVI+KDSAKKTAKKSRVK F+KLVNYQHLMPTRYTLDVDLK+  ++D L++KDKKVTAL             GKNRWFFTKLRF
Sbjct:   21 KKAVIIKSFDDGTSDRRYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALKSKDKKVTALKEAKAKLEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 42.743 bits (99), Expect = 1.391e-50
Identity = 20/22 (90.91%), Postives = 21/22 (95.45%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            MVKFLK NKAVI+LQGRYAGKK
Sbjct:    1 MVKFLKQNKAVILLQGRYAGKK 22

[Close]

BLAST of GR408812 vs. TAIR peptide
Match: AT2G32220.1 (| Symbols: | Ribosomal L27e protein family | chr2:13678945-13679352 FORWARD LENGTH=135)

HSP 1 Score: 172.555 bits (436), Expect = 1.639e-48
Identity = 83/115 (72.17%), Postives = 94/115 (81.74%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTALXXXXXXXXXXXXXGKNRWFFTKLRF 489
            KKAVIV++FDDGT EK YGHCLVAG+KKYPSKVI+KDSAKKTAKKSRVK F K++NYQH+MPTRYTLD+DLK+ VS D + +KDKKVTAL             GKNRWFFTKLRF
Sbjct:   21 KKAVIVKSFDDGTVEKKYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFFKVINYQHVMPTRYTLDLDLKNVVSADAISSKDKKVTALKEAKAKFEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 38.1206 bits (87), Expect = 1.639e-48
Identity = 17/22 (77.27%), Postives = 19/22 (86.36%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            MVK +KP KAVI+LQGRY GKK
Sbjct:    1 MVKCMKPGKAVILLQGRYTGKK 22

[Close]

BLAST of GR408812 vs. TAIR peptide
Match: AT4G15000.2 (| Symbols: | Ribosomal L27e protein family | chr4:8571896-8572387 FORWARD LENGTH=131)

HSP 1 Score: 156.377 bits (394), Expect = 4.633e-45
Identity = 72/90 (80.00%), Postives = 84/90 (93.33%), Query Frame = 1

Query:  145 KKAVIVRTFDDGTREKPYGHCLVAGIKKYPSKVIQKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVSVDVLQAKDKKVTAL 414
            KKAVI+++FDDG R++PYGHCLVAG+KKYPSKVI+KDSAKKTAKKSRVK F+KLVNYQHLMPTRYTLDVDLK+  ++D LQ+KDKKV AL
Sbjct:   21 KKAVIIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAAL 110

HSP 2 Score: 42.743 bits (99), Expect = 4.633e-45
Identity = 20/22 (90.91%), Postives = 21/22 (95.45%), Query Frame = 3

Query:   84 MVKFLKPNKAVIVLQGRYAGKK 149
            MVKFLK NKAVI+LQGRYAGKK
Sbjct:    1 MVKFLKQNKAVILLQGRYAGKK 22

The following BLAST results are available for this feature:

BLAST of GR408812 vs. SwissProt
BLAST of GR408812 vs. TrEMBL
BLAST of GR408812 vs. TAIR peptide

BLAST of GR408812 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
RL27_PEA	1.780e-55	83.48	60S ribosomal protein L27 OS=Pisum sativum GN=RPL2...	[more]
RL273_ARATH	1.220e-50	73.04	60S ribosomal protein L27-3 OS=Arabidopsis thalian...	[more]
RL272_ARATH	1.325e-49	72.17	60S ribosomal protein L27-2 OS=Arabidopsis thalian...	[more]
RL271_ARATH	1.562e-47	72.17	60S ribosomal protein L27-1 OS=Arabidopsis thalian...	[more]
RL27_SOLTU	2.394e-45	68.91	60S ribosomal protein L27 OS=Solanum tuberosum GN=...	[more]
RL27_PYRST	3.362e-34	57.39	60S ribosomal protein L27 OS=Pyrobotrys stellata G...	[more]
RL27B_YEAST	2.052e-30	48.28	60S ribosomal protein L27-B OS=Saccharomyces cerev...	[more]
RL27A_YEAST	1.000e-29	47.41	60S ribosomal protein L27-A OS=Saccharomyces cerev...	[more]
RL27_HIPCM	4.875e-29	50.00	60S ribosomal protein L27 OS=Hippocampus comes GN=...	[more]
RL27_DANRE	6.348e-29	50.00	60S ribosomal protein L27 OS=Danio rerio GN=rpl27 ...	[more]

BLAST of GR408812 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TMU7_SOYBN	1.979e-54	82.61	60S ribosomal protein L27 OS=Glycine max PE=2 SV=1	[more]
C6T3R4_SOYBN	1.651e-53	81.74	60S ribosomal protein L27 OS=Glycine max PE=2 SV=1	[more]
B7FH51_MEDTR	1.378e-52	79.13	60S ribosomal protein L27 OS=Medicago truncatula P...	[more]
B7FN57_MEDTR	8.816e-52	77.39	60S ribosomal protein L27 OS=Medicago truncatula P...	[more]
A9PBR8_POPTR	2.768e-50	75.65	60S ribosomal protein L27 OS=Populus trichocarpa G...	[more]
A9PAT1_POPTR	4.703e-50	74.78	60S ribosomal protein L27 OS=Populus trichocarpa P...	[more]
A9P8D7_POPTR	4.703e-50	74.78	60S ribosomal protein L27 OS=Populus trichocarpa G...	[more]
Q0WRB8_ARATH	1.770e-49	73.04	60S ribosomal protein L27 OS=Arabidopsis thaliana ...	[more]
B9HD67_POPTR	2.307e-49	74.78	60S ribosomal protein L27 OS=Populus trichocarpa G...	[more]
D7MB93_ARALY	3.921e-49	73.04	60S ribosomal protein L27 OS=Arabidopsis lyrata su...	[more]

BLAST of GR408812 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT4G15000.1	1.280e-51	73.04	\| Symbols: \| Ribosomal L27e protein family \| chr4...	[more]
AT3G22230.1	1.391e-50	72.17	\| Symbols: \| Ribosomal L27e protein family \| chr3...	[more]
AT2G32220.1	1.639e-48	72.17	\| Symbols: \| Ribosomal L27e protein family \| chr2...	[more]
AT4G15000.2	4.633e-45	80.00	\| Symbols: \| Ribosomal L27e protein family \| chr4...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001141	Ribosomal protein L27e	PANTHER	PTHR10497	60S RIBOSOMAL PROTEIN L27E	coord: 10..124 score: 8
IPR001141	Ribosomal protein L27e	PFAM	PF01777	Ribosomal_L27e	coord: 41..124 score: 3.2
IPR014722	Translation protein SH3-like, subgroup	GENE3D	2.30.30.30	no description	coord: 8..100 score: 0
IPR018262	Ribosomal protein L27e, conserved site	PROSITE	PS01107	RIBOSOMAL_L27E	coord: 113..124 score
None	No IPR available	SEG	seg	seg	coord: 100..112 score
None	No IPR available	SEG	seg	seg	coord: 13..40 score: NAcoord: 76..85 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR408812 ID=GR408812; Name=GR408812; organism=Cicer arietinum; type=EST; length=611bp
GGATGCATCCCCTCGCACTGCGTCCTCAGAAAAAATCCACCCTTTGTTTC
TTTTGGTTGCGTTTATTGTAGCAACGAGAGACAATGGTGAAATTCTTGAA
GCCTAACAAAGCTGTGATTGTCCTGCAAGGCCGATATGCGGGAAAAAAAG
GCCGTGATTGTTCGTACATTTGATGATGGCACTCGTGAGAAACCGTATGG
ACACTGTCTTGTTGCAGGGATTAAGAAGTACCCAAGCAAAGTAATCCAGA
AGGACTCTGCAAAGAAGACAGCAAAGAAATCTAGGGTTAAAGCTTTCGTG
AAGCTTGTCAATTATCAGCACTTGATGCCCACACGTTATACCCTTGATGT
GGATCTGAAAGATGCTGTTTCTGTCGATGTTCTTCAAGCCAAGGACAAGA
AGGTGACAGCCCTTAAGGAGACAAAGAAGCGCTTTGAGGAGAGGTTTAAG
ACTGGCAAGAACAGGTGGTTCTTTACCAAGCTTCGATTCTGAAAATTTTG
TTTAATTTATTATGAATGAATGAACTTGTTGTGTTTCTTAGTTTCATTTC
AGAGATTTGTGATTATTATCAATACAAAAAGTTAGTGAACAATTTCACCA
ATTGTTGCTCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003735	structural constituent of ribosome

Vocabulary: **Cellular Component**
Term	Definition
GO:0005622	intracellular anatomical structure
GO:0005840	ribosome

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation

Vocabulary: **INTERPRO**
Term	Definition
IPR001141	Ribosomal_eL27
IPR014722	Rib_uL2_dom2
IPR018262	Ribosomal_L27e_CS

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