GR420472

EST Overview


Name	GR420472
Unique Name	GR420472
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	231

Libraries


Library Name	Type
LIBEST_024786 JG11 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR420472 vs. SwissProt
Match: HSP13_SOYBN (17.5 kDa class I heat shock protein OS=Glycine max GN=HSP17.5-M PE=3 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 1.600e-8
Identity = 41/80 (51.25%), Postives = 50/80 (62.50%), Query Frame = -3

Query:   14 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP-VTAIQIT 229
            +DRVL+ISGER +EKEDKND   R   +S  F  RFR      V+ VKA MENGVL       +PKE+  KP V AI+I+
Sbjct:   77 DDRVLQISGERNLEKEDKNDTWHRVERSSGNFMRRFRLPENAKVEQVKASMENGVLTV----TVPKEEVKKPDVKAIEIS 152

[Close]

BLAST of GR420472 vs. SwissProt
Match: HSP11_SOLLC (17.8 kDa class I heat shock protein OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 1.600e-8
Identity = 41/80 (51.25%), Postives = 50/80 (62.50%), Query Frame = -3

Query:   14 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP-VTAIQIT 229
            EDRVL+ISGER +EKEDKND   R   +S  F  RFR      +D VKA MENGVL       +PKE+  KP V +I+I+
Sbjct:   78 EDRVLQISGERNVEKEDKNDKWHRMERSSGKFMRRFRLPENAKMDQVKASMENGVLTV----TVPKEEVKKPEVKSIEIS 153

[Close]

BLAST of GR420472 vs. SwissProt
Match: HSP15_SOYBN (17.6 kDa class I heat shock protein OS=Glycine max GN=HSP17.6-L PE=3 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 2.089e-8
Identity = 40/77 (51.95%), Postives = 47/77 (61.04%), Query Frame = -3

Query:   32 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKED----DVKPV 229
            +DRVL+ISGER +EKEDKND   R   +S  F  RFR      V+ VKA MENGVL       IPKE+    DVKP+
Sbjct:   78 DDRVLQISGERNVEKEDKNDTWHRVDRSSGKFMRRFRLPENAKVEQVKACMENGVLTV----TIPKEEVKKSDVKPI 150

[Close]

BLAST of GR420472 vs. SwissProt
Match: HSP11_SOLPE (17.7 kDa class I heat shock protein OS=Solanum peruvianum PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 2.089e-8
Identity = 41/80 (51.25%), Postives = 50/80 (62.50%), Query Frame = -3

Query:   14 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP-VTAIQIT 229
            EDRVL+ISGER +EKEDKND   R   +S  F  RFR      +D VKA MENGVL       +PKE+  KP V +I+I+
Sbjct:   78 EDRVLQISGERNMEKEDKNDKWQRVERSSGKFMRRFRLPENAKMDQVKASMENGVLTV----TVPKEEMKKPDVKSIEIS 153

[Close]

BLAST of GR420472 vs. SwissProt
Match: HSP11_MEDSA (18.1 kDa class I heat shock protein (Fragment) OS=Medicago sativa GN=HSP18.1 PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 2.089e-8
Identity = 40/80 (50.00%), Postives = 50/80 (62.50%), Query Frame = -3

Query:   14 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP-VTAIQIT 229
            +DRVL+ISGER +EKEDKND   R   +S  F  RFR      +D VKA MENGVL       +PKE+  KP V +I+I+
Sbjct:   67 DDRVLQISGERNVEKEDKNDQWHRVERSSGKFMRRFRLPENAKMDQVKAAMENGVLTV----TVPKEEIKKPEVKSIEIS 142

[Close]

BLAST of GR420472 vs. SwissProt
Match: HSP16_SOYBN (18.5 kDa class I heat shock protein OS=Glycine max GN=HSP18.5-C PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.728e-8
Identity = 40/80 (50.00%), Postives = 50/80 (62.50%), Query Frame = -3

Query:   14 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP-VTAIQIT 229
            +D+VL+ISGER +EKEDKND   R   +S  F  RFR      V+ VKA MENGVL       +PKE+  KP V AI+I+
Sbjct:   85 DDKVLQISGERNVEKEDKNDTWHRVERSSGKFMRRFRLPENAKVEQVKASMENGVLTV----TVPKEEVKKPDVKAIEIS 160

[Close]

BLAST of GR420472 vs. SwissProt
Match: HSP14_SOYBN (17.5 kDa class I heat shock protein OS=Glycine max GN=HSP17.5-E PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.728e-8
Identity = 41/80 (51.25%), Postives = 50/80 (62.50%), Query Frame = -3

Query:   14 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP-VTAIQIT 229
            +DRVL+ISGER +EKEDKND   R   +S  F  RFR      V+ VKA MENGVL       +PKE+  KP V AI+I+
Sbjct:   78 DDRVLQISGERNVEKEDKNDTWHRVERSSGKFTRRFRLPENAKVNEVKASMENGVLTV----TVPKEEVKKPDVKAIEIS 153

[Close]

BLAST of GR420472 vs. SwissProt
Match: HSP12_SOLPE (17.6 kDa class I heat shock protein OS=Solanum peruvianum PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 4.654e-8
Identity = 40/80 (50.00%), Postives = 49/80 (61.25%), Query Frame = -3

Query:   14 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP-VTAIQIT 229
            EDRVL+ISGER +EKEDKND   R   +S  F  RFR      +D VKA MENGVL       +PKE+   P V +I+I+
Sbjct:   78 EDRVLQISGERNVEKEDKNDTWHRVERSSGKFMRRFRLPENAKMDQVKASMENGVLTV----TVPKEEVNNPDVKSIEIS 153

[Close]

BLAST of GR420472 vs. SwissProt
Match: HSP12_MEDSA (18.2 kDa class I heat shock protein OS=Medicago sativa GN=HSP18.2 PE=2 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 6.078e-8
Identity = 37/72 (51.39%), Postives = 44/72 (61.11%), Query Frame = -3

Query:   35 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP 229
            +DRVL+ISGER +EKEDKND   R   +S  F  RFR      +D VKA MENGVL       +PKE+  KP
Sbjct:   82 DDRVLQISGERSVEKEDKNDQWHRLERSSGKFMRRFRLPENAKMDQVKAAMENGVLTV----TVPKEEVKKP 149

[Close]

BLAST of GR420472 vs. SwissProt
Match: HS17B_ARATH (17.6 kDa class I heat shock protein 2 OS=Arabidopsis thaliana GN=HSP17.6B PE=2 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 2.310e-7
Identity = 37/73 (50.68%), Postives = 42/73 (57.53%), Query Frame = -3

Query:   32 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKPV 229
            ED VLKISGER +EKEDKND   R   +S  F  RFR      +D VKA MENGVL         K+ DVK +
Sbjct:   77 EDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMDQVKAAMENGVLTVTVPKAETKKADVKSI 149

[Close]

BLAST of GR420472 vs. TrEMBL
Match: Q41028_PEA (Pisum sativum 17.9 kDa heat shock protein (hsp17.9) (Fragment) OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.708e-8
Identity = 44/80 (55.00%), Postives = 47/80 (58.75%), Query Frame = -3

Query:   14 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP-VTAIQIT 229
            EDRVLKISGERK EKEDKND   R   +   F  RFR      VD VKA MENGVL       +PKE+  KP    IQIT
Sbjct:   79 EDRVLKISGERKTEKEDKNDTWHRVERSQGSFLRRFRLPENAKVDQVKAAMENGVLTV----TVPKEEVKKPEAKPIQIT 154

[Close]

BLAST of GR420472 vs. TrEMBL
Match: B9IQG7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_738820 PE=3 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 8.479e-8
Identity = 42/80 (52.50%), Postives = 50/80 (62.50%), Query Frame = -3

Query:   14 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP-VTAIQIT 229
            +DRVL+ISGER +EKEDKND   R   +S  F  RFR      VD VKA MENGVL       +PKE+  KP V AI+I+
Sbjct:   76 DDRVLQISGERNVEKEDKNDTWHRVERSSGKFLRRFRLPENAKVDQVKASMENGVLTV----TVPKEEVKKPDVKAIEIS 151

[Close]

BLAST of GR420472 vs. TrEMBL
Match: B9IQG8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_574257 PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.107e-7
Identity = 42/80 (52.50%), Postives = 50/80 (62.50%), Query Frame = -3

Query:   14 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP-VTAIQIT 229
            +DRVL+ISGER +EKEDKND   R   +S  F  RFR      VD VKA MENGVL       +PKE+  KP V AI+I+
Sbjct:   76 DDRVLQISGERNVEKEDKNDTWHRVERSSGKFVRRFRLPENAKVDQVKASMENGVLTV----TVPKEEVKKPDVKAIEIS 151

[Close]

BLAST of GR420472 vs. TrEMBL
Match: Q53E21_TOBAC (Cytosolic class I small heat shock protein 3C (Fragment) OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.446e-7
Identity = 41/80 (51.25%), Postives = 49/80 (61.25%), Query Frame = -3

Query:   14 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP-VTAIQIT 229
            +DRVL+ISGER +EKEDKND   R   +S  F  RFR      +D VKA MENGVL       +PKE+  KP V +I IT
Sbjct:   48 DDRVLQISGERNVEKEDKNDTWHRVERSSGKFMRRFRLPENAKMDQVKAAMENGVLTV----TVPKEEVKKPDVKSIDIT 123

[Close]

BLAST of GR420472 vs. TrEMBL
Match: C6T5U3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.446e-7
Identity = 42/80 (52.50%), Postives = 49/80 (61.25%), Query Frame = -3

Query:   14 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP-VTAIQIT 229
            +DRVL+ISGER +EKEDKND   R   +S  F  RFR      VD VKA MENGVL       +PKE+  KP V AI I+
Sbjct:   77 DDRVLQISGERNVEKEDKNDTWHRVERSSGKFMRRFRLPENAKVDQVKASMENGVLTV----TVPKEEIKKPDVKAIDIS 152

[Close]

BLAST of GR420472 vs. TrEMBL
Match: B7FMD9_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.446e-7
Identity = 42/80 (52.50%), Postives = 50/80 (62.50%), Query Frame = -3

Query:   14 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP-VTAIQIT 229
            +DRVL+ISGER  EKEDKND   R   +S  F  RFR      +D VKA MENGVLA      +PKE+  KP V AI+I+
Sbjct:   85 DDRVLQISGERNFEKEDKNDQWHRVERSSGKFMRRFRLPENAKMDQVKAAMENGVLAV----TVPKEEIKKPEVKAIEIS 160

[Close]

BLAST of GR420472 vs. TrEMBL
Match: Q53E26_TOBAC (Cytosolic class I small heat shock protein 3A (Fragment) OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.889e-7
Identity = 40/80 (50.00%), Postives = 50/80 (62.50%), Query Frame = -3

Query:   14 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP-VTAIQIT 229
            +DRVL+ISG+R +EKEDKND   R   +S  F  RFR      +D VKA MENGVL       +PKE+  KP V +I+IT
Sbjct:   48 DDRVLQISGDRNVEKEDKNDTWHRVERSSGKFMRRFRLPENAKMDQVKAAMENGVLTV----TVPKEEVKKPDVKSIEIT 123

[Close]

BLAST of GR420472 vs. TrEMBL
Match: O82010_SOLPE (Hsp20.1 protein OS=Solanum peruvianum PE=2 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.889e-7
Identity = 41/80 (51.25%), Postives = 50/80 (62.50%), Query Frame = -3

Query:   14 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP-VTAIQIT 229
            EDRVL+ISGER +EKEDKND   R   +S  F  RFR      +D VKA MENGVL       +PKE+  KP V +I+I+
Sbjct:   78 EDRVLQISGERNVEKEDKNDTWHRVERSSGKFMRRFRLPENAKMDQVKASMENGVLTV----TVPKEEVKKPDVKSIEIS 153

[Close]

BLAST of GR420472 vs. TrEMBL
Match: B9MYJ0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_679803 PE=3 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.889e-7
Identity = 41/80 (51.25%), Postives = 50/80 (62.50%), Query Frame = -3

Query:   14 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP-VTAIQIT 229
            +DRVL+ISGER +EKEDKND   R   +S  F  RFR      +D VKA MENGVL       +PKE+  KP V AI+I+
Sbjct:   83 DDRVLQISGERNVEKEDKNDAWHRVERSSGKFLRRFRLPENAKMDQVKASMENGVLTV----TVPKEEIKKPDVKAIEIS 158

[Close]

BLAST of GR420472 vs. TrEMBL
Match: B9MYI9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_679801 PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 2.467e-7
Identity = 41/81 (50.62%), Postives = 49/81 (60.49%), Query Frame = -3

Query:   14 EDRVLKISGERKIEKEDKNDVVREHVASRC--FFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKP-VTAIQIT 229
            +DRVL+ISGER +EKED+ND     V   C  F  RFR      +DHVKA MENGVL       +PKE+  KP V AI I+
Sbjct:   82 DDRVLQISGERNVEKEDQND-TWHRVERSCGKFLRRFRLPENAKMDHVKASMENGVLTV----TVPKEEVKKPEVKAIDIS 157

[Close]

BLAST of GR420472 vs. TAIR peptide
Match: AT2G29500.1 (| Symbols: | HSP20-like chaperones superfamily protein | chr2:12633279-12633740 REVERSE LENGTH=153)

HSP 1 Score: 54.299 bits (129), Expect = 1.795e-8
Identity = 37/73 (50.68%), Postives = 42/73 (57.53%), Query Frame = -3

Query:   32 EDRVLKISGERKIEKEDKNDV-VREHVASRCFFPRFR------VDHVKAVMENGVLAAGKC*GIPKEDDVKPV 229
            ED VLKISGER +EKEDKND   R   +S  F  RFR      +D VKA MENGVL         K+ DVK +
Sbjct:   77 EDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMDQVKAAMENGVLTVTVPKAETKKADVKSI 149

The following BLAST results are available for this feature:

BLAST of GR420472 vs. SwissProt
BLAST of GR420472 vs. TrEMBL
BLAST of GR420472 vs. TAIR peptide

BLAST of GR420472 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
HSP13_SOYBN	1.600e-8	51.25	17.5 kDa class I heat shock protein OS=Glycine max...	[more]
HSP11_SOLLC	1.600e-8	51.25	17.8 kDa class I heat shock protein OS=Solanum lyc...	[more]
HSP15_SOYBN	2.089e-8	51.95	17.6 kDa class I heat shock protein OS=Glycine max...	[more]
HSP11_SOLPE	2.089e-8	51.25	17.7 kDa class I heat shock protein OS=Solanum per...	[more]
HSP11_MEDSA	2.089e-8	50.00	18.1 kDa class I heat shock protein (Fragment) OS=...	[more]
HSP16_SOYBN	2.728e-8	50.00	18.5 kDa class I heat shock protein OS=Glycine max...	[more]
HSP14_SOYBN	2.728e-8	51.25	17.5 kDa class I heat shock protein OS=Glycine max...	[more]
HSP12_SOLPE	4.654e-8	50.00	17.6 kDa class I heat shock protein OS=Solanum per...	[more]
HSP12_MEDSA	6.078e-8	51.39	18.2 kDa class I heat shock protein OS=Medicago sa...	[more]
HS17B_ARATH	2.310e-7	50.68	17.6 kDa class I heat shock protein 2 OS=Arabidops...	[more]

BLAST of GR420472 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q41028_PEA	1.708e-8	55.00	Pisum sativum 17.9 kDa heat shock protein (hsp17.9...	[more]
B9IQG7_POPTR	8.479e-8	52.50	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9IQG8_POPTR	1.107e-7	52.50	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q53E21_TOBAC	1.446e-7	51.25	Cytosolic class I small heat shock protein 3C (Fra...	[more]
C6T5U3_SOYBN	1.446e-7	52.50	Putative uncharacterized protein OS=Glycine max PE...	[more]
B7FMD9_MEDTR	1.446e-7	52.50	Putative uncharacterized protein OS=Medicago trunc...	[more]
Q53E26_TOBAC	1.889e-7	50.00	Cytosolic class I small heat shock protein 3A (Fra...	[more]
O82010_SOLPE	1.889e-7	51.25	Hsp20.1 protein OS=Solanum peruvianum PE=2 SV=1	[more]
B9MYJ0_POPTR	1.889e-7	51.25	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9MYI9_POPTR	2.467e-7	50.62	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]

BLAST of GR420472 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT2G29500.1	1.795e-8	50.68	\| Symbols: \| HSP20-like chaperones superfamily pr...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 31..49 score
None	No IPR available	GENE3D	2.60.40.790	no description	coord: 2..49 score: 1.8
IPR002068	Heat shock protein Hsp20	PFAM	PF00011	HSP20	coord: 1..49 score: 4.4

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR420472 ID=GR420472; Name=GR420472; organism=Cicer arietinum; type=EST; length=231bp
GGCTTCTTAACTCCGTAATCTGAATGGCAGTAACAGGCTTAACATCGTCC
TCCTTAGGAATACCTTAACATTTCCCGGCAGCCAGCACACCATTTTCCAT
AACAGCTTTCACATGGTCCACTCTAAAACGGGGGAAAAAACAACGCGATG
CCACGTGTTCCCTGACTACGTCGTTCTTGTCTTCCTTTTCAATTTTCCTC
TCCCCGCTTATTTTCAAGACGCGATCCTCTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR002068	A-crystallin/Hsp20_dom

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