HO062346

EST Overview


Name	HO062346
Unique Name	HO062346
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	679

Libraries


Library Name	Type
LIBEST_026408 Chickpea drought stressed cDNA SSH library AB1	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO062346 vs. TrEMBL
Match: B7FN91_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 9.337e-39
Identity = 79/82 (96.34%), Postives = 81/82 (98.78%), Query Frame = -2

Query:  256 MSLDLQVLINGLKHFASLNVKSKLQMVETFIKAYYLPETEYVHWARGHPEYSKSQVTGLINLVASMKGWKRKTRLEILEKIE 501
            MSLDLQVLINGLKHFASLNVKSKLQMVETFIKAYYLPETEYVHWARGHPEYSKSQV GL+NLVASMKGWKRKTRLE+LEKIE
Sbjct:  160 MSLDLQVLINGLKHFASLNVKSKLQMVETFIKAYYLPETEYVHWARGHPEYSKSQVVGLVNLVASMKGWKRKTRLEVLEKIE 241

[Close]

BLAST of HO062346 vs. TrEMBL
Match: Q6JJ34_IPOTF (Putative uncharacterized protein OS=Ipomoea trifida PE=4 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 4.059e-34
Identity = 69/82 (84.15%), Postives = 78/82 (95.12%), Query Frame = -2

Query:  256 MSLDLQVLINGLKHFASLNVKSKLQMVETFIKAYYLPETEYVHWARGHPEYSKSQVTGLINLVASMKGWKRKTRLEILEKIE 501
            MSLDLQVLINGLKHF S++V+ KLQ+VETFIKAYYLPETE+VHW+R HPEYSKSQV GL+NLVA+MKGWKRKTRLE+LEKIE
Sbjct: 1163 MSLDLQVLINGLKHFVSVDVRPKLQIVETFIKAYYLPETEFVHWSRAHPEYSKSQVVGLVNLVATMKGWKRKTRLEVLEKIE 1244

HSP 2 Score: 72.4034 bits (176), Expect = 3.697e-11
Identity = 32/44 (72.73%), Postives = 38/44 (86.36%), Query Frame = -1

Query:  545 QNSRLTYSDLCLQIPDPKFRQCLLRTLAVLFDLMCSYYEIMDFQ 676
            Q +RLTYSDLC+Q+P+ KFRQCLL TLAVLF LMCSY+ I+ FQ
Sbjct:  378 QGARLTYSDLCIQMPESKFRQCLLATLAVLFKLMCSYHAILSFQ 421

[Close]

BLAST of HO062346 vs. TrEMBL
Match: B9SCM8_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0476140 PE=4 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 4.059e-34
Identity = 69/82 (84.15%), Postives = 77/82 (93.90%), Query Frame = -2

Query:  256 MSLDLQVLINGLKHFASLNVKSKLQMVETFIKAYYLPETEYVHWARGHPEYSKSQVTGLINLVASMKGWKRKTRLEILEKIE 501
            MSLDLQVLINGL+HF  +NVK KLQ+VETFIKAYYLPETEYVHWAR HPEY+K+Q+ GLINLVA+MKGWKRKTRLE+LEKIE
Sbjct: 1029 MSLDLQVLINGLQHFVPVNVKPKLQIVETFIKAYYLPETEYVHWARAHPEYTKNQIVGLINLVATMKGWKRKTRLEVLEKIE 1110

HSP 2 Score: 85.1149 bits (209), Expect = 5.512e-15
Identity = 41/50 (82.00%), Postives = 44/50 (88.00%), Query Frame = -1

Query:  527 QNSRLTYSDLCLQIPDPKFRQCLLRTLAVLFDLMCSYYEIMDFQLERKDS 676
            QNSRLTYSDLCLQIP+ KFRQCLLRTLAVLF LMCSY+EIM F +E K S
Sbjct:  370 QNSRLTYSDLCLQIPESKFRQCLLRTLAVLFRLMCSYHEIMIFHIENKVS 419

[Close]

BLAST of HO062346 vs. TrEMBL
Match: B9HQR3_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_199559 PE=4 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 4.059e-34
Identity = 69/82 (84.15%), Postives = 76/82 (92.68%), Query Frame = -2

Query:  256 MSLDLQVLINGLKHFASLNVKSKLQMVETFIKAYYLPETEYVHWARGHPEYSKSQVTGLINLVASMKGWKRKTRLEILEKIE 501
            MSLDLQVLINGL+HF  +NVK KLQMVETFIKAYYLPETEYVHWAR HPEY K+Q+ GLINLVA+MKGWKRKTRLE++EKIE
Sbjct:   55 MSLDLQVLINGLQHFVHVNVKPKLQMVETFIKAYYLPETEYVHWARAHPEYRKNQIVGLINLVATMKGWKRKTRLEVIEKIE 136

[Close]

BLAST of HO062346 vs. TrEMBL
Match: A0A934_IPOTF (Putative uncharacterized protein OS=Ipomoea trifida PE=4 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 4.059e-34
Identity = 69/82 (84.15%), Postives = 78/82 (95.12%), Query Frame = -2

Query:  256 MSLDLQVLINGLKHFASLNVKSKLQMVETFIKAYYLPETEYVHWARGHPEYSKSQVTGLINLVASMKGWKRKTRLEILEKIE 501
            MSLDLQVLINGLKHF S++V+ KLQ+VETFIKAYYLPETE+VHW+R HPEYSKSQV GL+NLVA+MKGWKRKTRLE+LEKIE
Sbjct:   93 MSLDLQVLINGLKHFVSVDVRPKLQIVETFIKAYYLPETEFVHWSRAHPEYSKSQVVGLVNLVATMKGWKRKTRLEVLEKIE 174

[Close]

BLAST of HO062346 vs. TrEMBL
Match: B9GEV0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_829534 PE=4 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 9.043e-34
Identity = 68/82 (82.93%), Postives = 76/82 (92.68%), Query Frame = -2

Query:  256 MSLDLQVLINGLKHFASLNVKSKLQMVETFIKAYYLPETEYVHWARGHPEYSKSQVTGLINLVASMKGWKRKTRLEILEKIE 501
            MSLDLQVLINGL+HF  +NVK KLQMVE FIKAYYLPETEYVHWAR HPEY+K+Q+ GLINLVA+MKGWKRKTRLE++EKIE
Sbjct: 1025 MSLDLQVLINGLQHFVPVNVKPKLQMVEAFIKAYYLPETEYVHWARAHPEYTKNQIVGLINLVAAMKGWKRKTRLEVIEKIE 1106

HSP 2 Score: 84.3445 bits (207), Expect = 9.402e-15
Identity = 44/86 (51.16%), Postives = 58/86 (67.44%), Query Frame = -1

Query:  431 QNSRLTYSDLCLQIPDPKFRQCLLRTLAVLFDLMCSYYEIMDFQLERKDSVAQTSDK----CNVIGSAGIDKWAKAFCFVECEVQA 676
            QNSRLTYSDL LQIP+ KFRQCLLRTLAVLF L+ SY+EIM+FQLE KDS+            ++GS+  ++    + +++    A
Sbjct:  381 QNSRLTYSDLSLQIPESKFRQCLLRTLAVLFRLISSYHEIMNFQLENKDSLGSNGSPRESVDRMLGSSPTEESTTTYMYLDSNFDA 466

[Close]

BLAST of HO062346 vs. TrEMBL
Match: D7SN87_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_61.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00031444001 PE=4 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 2.631e-33
Identity = 68/82 (82.93%), Postives = 76/82 (92.68%), Query Frame = -2

Query:  256 MSLDLQVLINGLKHFASLNVKSKLQMVETFIKAYYLPETEYVHWARGHPEYSKSQVTGLINLVASMKGWKRKTRLEILEKIE 501
            MSLDLQVLINGL+HF S NVK KLQ+VE FIKAYYLPETEYVHWAR HPEYSK+Q+ GLINLVA+++GWKRKTRLE+LEKIE
Sbjct:  979 MSLDLQVLINGLQHFVSANVKPKLQIVEIFIKAYYLPETEYVHWARAHPEYSKNQIVGLINLVATVRGWKRKTRLEVLEKIE 1060

HSP 2 Score: 74.3294 bits (181), Expect = 9.729e-12
Identity = 36/48 (75.00%), Postives = 40/48 (83.33%), Query Frame = -1

Query:  533 QNSRLTYSDLCLQIPDPKFRQCLLRTLAVLFDLMCSYYEIMDFQLERK 676
            Q+SRLTYSDLCL+IP+ KFR CLL+TLA LF LM SYY IM FQLE K
Sbjct:  323 QSSRLTYSDLCLRIPESKFRLCLLKTLAGLFRLMSSYYAIMSFQLENK 370

[Close]

BLAST of HO062346 vs. TrEMBL
Match: Q9SJK0_ARATH (Putative uncharacterized protein At2g27910 OS=Arabidopsis thaliana GN=At2g27910 PE=2 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 5.862e-33
Identity = 67/82 (81.71%), Postives = 75/82 (91.46%), Query Frame = -2

Query:  256 MSLDLQVLINGLKHFASLNVKSKLQMVETFIKAYYLPETEYVHWARGHPEYSKSQVTGLINLVASMKGWKRKTRLEILEKIE 501
            MSLDLQVLINGL+HF    VK KLQ+VETFIKAYYLPETEYVHWAR HPEY+K+QV GL+NLVA+MKGWKRKTRLE++EKIE
Sbjct:   55 MSLDLQVLINGLQHFVPTKVKPKLQIVETFIKAYYLPETEYVHWARAHPEYTKAQVVGLVNLVATMKGWKRKTRLEVIEKIE 136

[Close]

BLAST of HO062346 vs. TrEMBL
Match: Q8RXC2_ARATH (At2g27890 OS=Arabidopsis thaliana GN=At2g27890:At2g27900:At2g27910 PE=2 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 5.862e-33
Identity = 67/82 (81.71%), Postives = 75/82 (91.46%), Query Frame = -2

Query:  256 MSLDLQVLINGLKHFASLNVKSKLQMVETFIKAYYLPETEYVHWARGHPEYSKSQVTGLINLVASMKGWKRKTRLEILEKIE 501
            MSLDLQVLINGL+HF    VK KLQ+VETFIKAYYLPETEYVHWAR HPEY+K+QV GL+NLVA+MKGWKRKTRLE++EKIE
Sbjct: 1039 MSLDLQVLINGLQHFVPTKVKPKLQIVETFIKAYYLPETEYVHWARAHPEYTKAQVVGLVNLVATMKGWKRKTRLEVIEKIE 1120

HSP 2 Score: 73.559 bits (179), Expect = 1.660e-11
Identity = 41/70 (58.57%), Postives = 49/70 (70.00%), Query Frame = -1

Query:  485 QNSRLTYSDLCLQIPDPKFRQCLLRTLAVLFDLMCSYYEIMDFQLERK------DSVAQTSDKCNVIGSA 676
            Q SRLTYSDLCLQ P+ KFRQCLLRTLAVLF L+ SY+EIM F  E++       S+A T    +V GS+
Sbjct:  379 QYSRLTYSDLCLQTPESKFRQCLLRTLAVLFQLIYSYHEIMSFTPEKEAEILVSPSLATTQMVDSVTGSS 448

[Close]

BLAST of HO062346 vs. TrEMBL
Match: B9DFD2_ARATH (AT2G27900 protein OS=Arabidopsis thaliana GN=AT2G27900 PE=2 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 5.862e-33
Identity = 67/82 (81.71%), Postives = 75/82 (91.46%), Query Frame = -2

Query:  256 MSLDLQVLINGLKHFASLNVKSKLQMVETFIKAYYLPETEYVHWARGHPEYSKSQVTGLINLVASMKGWKRKTRLEILEKIE 501
            MSLDLQVLINGL+HF    VK KLQ+VETFIKAYYLPETEYVHWAR HPEY+K+QV GL+NLVA+MKGWKRKTRLE++EKIE
Sbjct: 1039 MSLDLQVLINGLQHFVPTKVKPKLQIVETFIKAYYLPETEYVHWARAHPEYTKAQVVGLVNLVATMKGWKRKTRLEVIEKIE 1120

HSP 2 Score: 73.559 bits (179), Expect = 1.660e-11
Identity = 41/70 (58.57%), Postives = 49/70 (70.00%), Query Frame = -1

Query:  485 QNSRLTYSDLCLQIPDPKFRQCLLRTLAVLFDLMCSYYEIMDFQLERK------DSVAQTSDKCNVIGSA 676
            Q SRLTYSDLCLQ P+ KFRQCLLRTLAVLF L+ SY+EIM F  E++       S+A T    +V GS+
Sbjct:  379 QYSRLTYSDLCLQTPESKFRQCLLRTLAVLFQLIYSYHEIMSFTPEKEAEILVSPSLATTQMVDSVTGSS 448

[Close]

BLAST of HO062346 vs. TAIR peptide
Match: AT2G27900.2 (| Symbols: | CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2451, C-terminal (InterPro:IPR019514), Vacuolar protein sorting-associated protein 54 (InterPro:IPR019515); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). | chr2:11877654-11885238 FORWARD LENGTH=1124)

HSP 1 Score: 144.821 bits (364), Expect = 4.021e-35
Identity = 67/82 (81.71%), Postives = 75/82 (91.46%), Query Frame = -2

Query:  256 MSLDLQVLINGLKHFASLNVKSKLQMVETFIKAYYLPETEYVHWARGHPEYSKSQVTGLINLVASMKGWKRKTRLEILEKIE 501
            MSLDLQVLINGL+HF    VK KLQ+VETFIKAYYLPETEYVHWAR HPEY+K+QV GL+NLVA+MKGWKRKTRLE++EKIE
Sbjct: 1039 MSLDLQVLINGLQHFVPTKVKPKLQIVETFIKAYYLPETEYVHWARAHPEYTKAQVVGLVNLVATMKGWKRKTRLEVIEKIE 1120

HSP 2 Score: 73.559 bits (179), Expect = 1.138e-13
Identity = 41/70 (58.57%), Postives = 49/70 (70.00%), Query Frame = -1

Query:  485 QNSRLTYSDLCLQIPDPKFRQCLLRTLAVLFDLMCSYYEIMDFQLERK------DSVAQTSDKCNVIGSA 676
            Q SRLTYSDLCLQ P+ KFRQCLLRTLAVLF L+ SY+EIM F  E++       S+A T    +V GS+
Sbjct:  379 QYSRLTYSDLCLQTPESKFRQCLLRTLAVLFQLIYSYHEIMSFTPEKEAEILVSPSLATTQMVDSVTGSS 448

[Close]

BLAST of HO062346 vs. TAIR peptide
Match: AT2G27900.1 (| Symbols: | CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2451, C-terminal (InterPro:IPR019514), Vacuolar protein sorting-associated protein 54 (InterPro:IPR019515); Has 316 Blast hits to 252 proteins in 92 species: Archae - 0; Bacteria - 2; Metazoa - 200; Fungi - 2; Plants - 68; Viruses - 0; Other Eukaryotes - 44 (source: NCBI BLink). | chr2:11877654-11885238 FORWARD LENGTH=1124)

HSP 1 Score: 144.821 bits (364), Expect = 4.021e-35
Identity = 67/82 (81.71%), Postives = 75/82 (91.46%), Query Frame = -2

Query:  256 MSLDLQVLINGLKHFASLNVKSKLQMVETFIKAYYLPETEYVHWARGHPEYSKSQVTGLINLVASMKGWKRKTRLEILEKIE 501
            MSLDLQVLINGL+HF    VK KLQ+VETFIKAYYLPETEYVHWAR HPEY+K+QV GL+NLVA+MKGWKRKTRLE++EKIE
Sbjct: 1039 MSLDLQVLINGLQHFVPTKVKPKLQIVETFIKAYYLPETEYVHWARAHPEYTKAQVVGLVNLVATMKGWKRKTRLEVIEKIE 1120

HSP 2 Score: 73.559 bits (179), Expect = 1.138e-13
Identity = 41/70 (58.57%), Postives = 49/70 (70.00%), Query Frame = -1

Query:  485 QNSRLTYSDLCLQIPDPKFRQCLLRTLAVLFDLMCSYYEIMDFQLERK------DSVAQTSDKCNVIGSA 676
            Q SRLTYSDLCLQ P+ KFRQCLLRTLAVLF L+ SY+EIM F  E++       S+A T    +V GS+
Sbjct:  379 QYSRLTYSDLCLQTPESKFRQCLLRTLAVLFQLIYSYHEIMSFTPEKEAEILVSPSLATTQMVDSVTGSS 448

The following BLAST results are available for this feature:

BLAST of HO062346 vs. TrEMBL
BLAST of HO062346 vs. TAIR peptide

BLAST of HO062346 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FN91_MEDTR	9.337e-39	96.34	Putative uncharacterized protein OS=Medicago trunc...	[more]
Q6JJ34_IPOTF	4.059e-34	84.15	Putative uncharacterized protein OS=Ipomoea trifid...	[more]
B9SCM8_RICCO	4.059e-34	84.15	Putative uncharacterized protein OS=Ricinus commun...	[more]
B9HQR3_POPTR	4.059e-34	84.15	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
A0A934_IPOTF	4.059e-34	84.15	Putative uncharacterized protein OS=Ipomoea trifid...	[more]
B9GEV0_POPTR	9.043e-34	82.93	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7SN87_VITVI	2.631e-33	82.93	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q9SJK0_ARATH	5.862e-33	81.71	Putative uncharacterized protein At2g27910 OS=Arab...	[more]
Q8RXC2_ARATH	5.862e-33	81.71	At2g27890 OS=Arabidopsis thaliana GN=At2g27890:At2...	[more]
B9DFD2_ARATH	5.862e-33	81.71	AT2G27900 protein OS=Arabidopsis thaliana GN=AT2G2...	[more]

BLAST of HO062346 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT2G27900.2	4.021e-35	81.71	\| Symbols: \| CONTAINS InterPro DOMAIN/s: Protein ...	[more]
AT2G27900.1	4.021e-35	81.71	\| Symbols: \| CONTAINS InterPro DOMAIN/s: Protein ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 1..8 score
None	No IPR available	PANTHER	PTHR13258	UNCHARACTERIZED	coord: 8..89 score: 1.2
IPR019514	Protein of unknown function DUF2451, C-terminal	PFAM	PF10474	DUF2451	coord: 7..87 score: 9.5
IPR019515	Vacuolar protein sorting-associated protein 54	PFAM	PF10475	DUF2450	coord: 2..44 score: 4.7

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO062346 ID=HO062346; Name=HO062346; organism=Cicer arietinum; type=EST; length=679bp
GACCCAAGTTATGTAAAACTTAAAAACGAAAAAATTATTGTACAACTACA
GCTTATTTTCAAATAACTATATCTACAGGATACAGGAAGCACGAATTCTG
TAAATGTTAAATAATTAATAGGGATCAAATACCATGATATTATACACTAC
CTTTACACAGAGCTCTGCCAAAAATGAAAGAAGAAGAAAAGGAGAAGAAG
ATTCTTCCATATGCGTGACTTCATTCATACACAACAAAAGACAGCTAATC
ACTCATTCAATCTTTTCCAGTATTTCCAACCTGGTTTTCCTCTTCCAACC
TTTCATAGAGGCAACCAGGTTGATCAGTCCAGTAACCTGGCTTTTGCTGT
ATTCCGGGTGCCCACGTGCCCAGTGCACATATTCAGTCTCGGGAAGGTAG
TATGCCTTAATGAAGGTTTCAACCATTTGTAGCTTGGACTTCACATTCAA
CGAAGCAAAATGCTTTAGCCCATTTATCAATACCTGCAGATCCAATGACA
TTGCACTTATCGGAAGTTTGTGCTACTGAGTCCTTTCTCTCTAGCTGAAA
ATCCATAATTTCATAGTATGAACACATAAGGTCAAATAAAACAGCCAATG
TTCTTAGTAAGCACTGCCTAAACTTAGGATCAGGTATCTGAAGGCATAGA
TCGCTGTAAGTGAGCCTACTATTTTGCGC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR019514	Syndetin_C
IPR019515	VPS54_N

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