HO062971

EST Overview


Name	HO062971
Unique Name	HO062971
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	636

Libraries


Library Name	Type
LIBEST_026409 Chickpea drought stressed cDNA SSH library AB2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO062971 vs. TrEMBL
Match: Q40366_MEDSA (Peroxidase OS=Medicago sativa GN=pxdC PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.488e-8
Identity = 31/42 (73.81%), Postives = 34/42 (80.95%), Query Frame = -1

Query:  193 TSIFNNNSLRXWDVVNKIKTAGENVCPNTVSCXDILALAAHI 318
            T+  NNNS+R  DVVN+IKTA EN CPNTVSC DILALAA I
Sbjct:   94 TAFGNNNSIRGLDVVNQIKTAVENACPNTVSCADILALAAEI 135

[Close]

BLAST of HO062971 vs. TrEMBL
Match: B7FI14_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.488e-8
Identity = 31/42 (73.81%), Postives = 34/42 (80.95%), Query Frame = -1

Query:  193 TSIFNNNSLRXWDVVNKIKTAGENVCPNTVSCXDILALAAHI 318
            T+  NNNS+R  DVVN+IKTA EN CPNTVSC DILALAA I
Sbjct:   93 TAFGNNNSIRGLDVVNQIKTAVENACPNTVSCADILALAAEI 134

[Close]

BLAST of HO062971 vs. TrEMBL
Match: B7FF82_MEDTR (Putative uncharacterized protein (Fragment) OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.488e-8
Identity = 31/42 (73.81%), Postives = 34/42 (80.95%), Query Frame = -1

Query:  193 TSIFNNNSLRXWDVVNKIKTAGENVCPNTVSCXDILALAAHI 318
            T+  NNNS+R  DVVN+IKTA EN CPNTVSC DILALAA I
Sbjct:   93 TAFGNNNSIRGLDVVNQIKTAVENACPNTVSCADILALAAEI 134

[Close]

BLAST of HO062971 vs. TrEMBL
Match: Q18PQ9_PEA (Peroxidase OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 2.538e-8
Identity = 33/52 (63.46%), Postives = 37/52 (71.15%), Query Frame = -1

Query:  175 TSIFNNNSLRXWDVVNKIKTAGENVCPNTVSCXDILALAAHI----YQGRVW 318
            T+  NNNS+R  DVVN+IKTA EN CPNTVSC DILAL+A I     QG  W
Sbjct:   95 TAAPNNNSIRGLDVVNQIKTAVENACPNTVSCADILALSAEISSDLAQGPTW 146

[Close]

BLAST of HO062971 vs. TrEMBL
Match: Q93XK6_MEDSA (Peroxidase1A OS=Medicago sativa GN=prx1A PE=3 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 9.643e-8
Identity = 28/38 (73.68%), Postives = 32/38 (84.21%), Query Frame = -1

Query:  193 NNNSLRXWDVVNKIKTAGENVCPNTVSCXDILALAAHI 306
            NNNS+R  DV+N+IKTA EN CPNTVSC DILAL+A I
Sbjct:   97 NNNSIRGLDVINQIKTAVENACPNTVSCADILALSAEI 134

[Close]

BLAST of HO062971 vs. TrEMBL
Match: O24081_MEDSA (Peroxidase1A OS=Medicago sativa GN=prx1A PE=2 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 9.643e-8
Identity = 28/38 (73.68%), Postives = 32/38 (84.21%), Query Frame = -1

Query:  193 NNNSLRXWDVVNKIKTAGENVCPNTVSCXDILALAAHI 306
            NNNS+R  DV+N+IKTA EN CPNTVSC DILAL+A I
Sbjct:   95 NNNSIRGLDVINQIKTAVENACPNTVSCADILALSAEI 132

[Close]

BLAST of HO062971 vs. TrEMBL
Match: B7FKI7_MEDTR (Putative uncharacterized protein (Fragment) OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.259e-7
Identity = 32/48 (66.67%), Postives = 35/48 (72.92%), Query Frame = -1

Query:  175 NNNSLRXWDVVNKIKTAGENVCPNTVSCXDILALAAH----IYQGRVW 306
            NNNSLR  DVVN+IKTA E+ CPNTVSC DILALAA     + QG  W
Sbjct:   96 NNNSLRGLDVVNQIKTAIESACPNTVSCADILALAAQASSVLAQGPSW 143

[Close]

BLAST of HO062971 vs. TrEMBL
Match: Q18PQ8_PEA (Peroxidase OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.645e-7
Identity = 30/38 (78.95%), Postives = 32/38 (84.21%), Query Frame = -1

Query:  193 NNNSLRXWDVVNKIKTAGENVCPNTVSCXDILALAAHI 306
            N NSLR  DVVNKIKTA E+VCPNTVSC DILALAA +
Sbjct:   95 NINSLRGLDVVNKIKTAVESVCPNTVSCADILALAAEL 132

[Close]

BLAST of HO062971 vs. TrEMBL
Match: Q40365_MEDSA (Peroxidase OS=Medicago sativa GN=pxdA PE=2 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 3.664e-7
Identity = 31/47 (65.96%), Postives = 35/47 (74.47%), Query Frame = -1

Query:  175 NNNSLRXWDVVNKIKTAGENVCPNTVSCXDILALA---AHIYQGRVW 306
            NNNSLR  DVVN+IKTA E+ CPNTVSC DILALA   + + QG  W
Sbjct:   89 NNNSLRGLDVVNQIKTAVESACPNTVSCADILALAQASSVLAQGPSW 135

[Close]

BLAST of HO062971 vs. TrEMBL
Match: Q76KW0_PEA (Peroxidase (Fragment) OS=Pisum sativum GN=PRX PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 4.786e-7
Identity = 28/38 (73.68%), Postives = 31/38 (81.58%), Query Frame = -1

Query:  193 NNNSLRXWDVVNKIKTAGENVCPNTVSCXDILALAAHI 306
            N NSLR  DV+N+IKTA EN CPNTVSC DILAL+A I
Sbjct:   18 NINSLRGLDVINRIKTAVENACPNTVSCADILALSAQI 55

The following BLAST results are available for this feature:

BLAST of HO062971 vs. TrEMBL

BLAST of HO062971 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q40366_MEDSA	1.488e-8	73.81	Peroxidase OS=Medicago sativa GN=pxdC PE=2 SV=1	[more]
B7FI14_MEDTR	1.488e-8	73.81	Putative uncharacterized protein OS=Medicago trunc...	[more]
B7FF82_MEDTR	1.488e-8	73.81	Putative uncharacterized protein (Fragment) OS=Med...	[more]
Q18PQ9_PEA	2.538e-8	63.46	Peroxidase OS=Pisum sativum PE=2 SV=1	[more]
Q93XK6_MEDSA	9.643e-8	73.68	Peroxidase1A OS=Medicago sativa GN=prx1A PE=3 SV=1	[more]
O24081_MEDSA	9.643e-8	73.68	Peroxidase1A OS=Medicago sativa GN=prx1A PE=2 SV=1	[more]
B7FKI7_MEDTR	1.259e-7	66.67	Putative uncharacterized protein (Fragment) OS=Med...	[more]
Q18PQ8_PEA	1.645e-7	78.95	Peroxidase OS=Pisum sativum PE=2 SV=1	[more]
Q40365_MEDSA	3.664e-7	65.96	Peroxidase OS=Medicago sativa GN=pxdA PE=2 SV=1	[more]
Q76KW0_PEA	4.786e-7	73.68	Peroxidase (Fragment) OS=Pisum sativum GN=PRX PE=2...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 31..49 score: NAcoord: 59..68 score
None	No IPR available	SEG	seg	seg	coord: 32..41 score
None	No IPR available	SEG	seg	seg	coord: 53..63 score
None	No IPR available	SEG	seg	seg	coord: 3..21 score
None	No IPR available	SEG	seg	seg	coord: 9..23 score: NAcoord: 41..49 score
None	No IPR available	GENE3D	1.10.520.10	no description	coord: 13..50 score: 4.6
IPR002016	Haem peroxidase, plant/fungal/bacterial	PFAM	PF00141	peroxidase	coord: 14..50 score: 9
IPR002016	Haem peroxidase, plant/fungal/bacterial	PROFILE	PS50873	PEROXIDASE_4	coord: 15..49 score: 10
IPR010255	Haem peroxidase	SUPERFAMILY	48113	Heme-dependent peroxidases	coord: 14..51 score: 6.8

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO062971 ID=HO062971; Name=HO062971; organism=Cicer arietinum; type=EST; length=636bp
CCAACACCACTCAAAGAAAGATTTCAAATACAAGAAAATTTTCCCCCCCA
AAGACTTATACCAATAACTATCTGACACATACCATGAATGTGTGCCCAGT
TATAGCTTCTTTATTAGGACCTCCAATCACTACATTTCAAGAGTAGTGAC
CCTAATACATTTACATTGAGCTAACCATACCCTCCCCTGATAAATATGTG
CAGCAAGGGCCAAAATATCANCACAAGAAACTGTATTAGGACAAACATTT
TCCCCCGCTGTTTTTATTTTGTTCACCACATCCCAACNTCTTAAGGAATT
GTTATTGAAAATGCTTGTTGTTCCCTCCCCACCCCCCCCCATTATTTAAA
AAACTGACCCCCCCCAACCTTGAAACAAACATCATGAAAGTGAAATCCAA
CCANACAGCAAGCATCCGAGGATTGTCCTTAAACCTTTCCTANGACTCCC
CCCAACCATGGAAAGACCTTACGACAATATTTCCTTAAAGGAGGGGTCAA
GTTGTGCCCTCCCAAGGAGAAGGGGAAACCCCCCCAANACAACCCCCACC
CACCCCAAAACTGTCAAGGTGGAAATCAAAAAACTTTCTTTTTAAATTGT
TGGGAGTCCCCCCCAACCTCCCCCCCCGCCCCCCCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004601	peroxidase activity
GO:0020037	heme binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006979	response to oxidative stress
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR002016	Haem_peroxidase
IPR010255	Haem_peroxidase_sf

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