HO067341

EST Overview


Name	HO067341
Unique Name	HO067341
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	435

Libraries


Library Name	Type
LIBEST_026414 Chickpea drought stressed cDNA SSH library BULK1	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of HO067341 vs. SwissProt
Match: AVP_PHAAU (Pyrophosphate-energized vacuolar membrane proton pump OS=Phaseolus aureus PE=1 SV=3)

HSP 1 Score: 244.973 bits (624), Expect = 9.758e-65
Identity = 122/139 (87.77%), Postives = 131/139 (94.24%), Query Frame = 2

Query:   17 EEEGINDHNVVLKCAEIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLLAA 433
            EEEGINDHNVV+KCAEIQNAISEGATSFLFT Y YVGIFMVAFA+LIFLFLGSVEGFST  Q C+YD+TK CKPALATA+FST+SF+LGGVTS++SGFLGMKIA YANARTTLEARK VGKAFITAFRSGAVMGFLLAA
Sbjct:   61 EEEGINDHNVVVKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAILIFLFLGSVEGFSTSPQACSYDKTKTCKPALATAIFSTVSFLLGGVTSLVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGFLLAA 199

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BLAST of HO067341 vs. SwissProt
Match: AVP1_ARATH (Pyrophosphate-energized vacuolar membrane proton pump 1 OS=Arabidopsis thaliana GN=AVP1 PE=1 SV=1)

HSP 1 Score: 226.868 bits (577), Expect = 2.750e-59
Identity = 113/139 (81.29%), Postives = 123/139 (88.49%), Query Frame = 2

Query:   17 EEEGINDHNVVLKCAEIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLLAA 433
            EEEG+ND +VV KCAEIQ AISEGATSFLFT Y YVG+FM+ FA +IF+FLGSVEGFST  +PCTYD T+ CKPALATA FSTI+FVLG VTSV+SGFLGMKIA YANARTTLEARK VGKAFI AFRSGAVMGFLLAA
Sbjct:   65 EEEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDNKPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGKAFIVAFRSGAVMGFLLAA 203

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BLAST of HO067341 vs. SwissProt
Match: AVP_HORVU (Pyrophosphate-energized vacuolar membrane proton pump OS=Hordeum vulgare PE=2 SV=2)

HSP 1 Score: 224.942 bits (572), Expect = 1.045e-58
Identity = 112/139 (80.58%), Postives = 125/139 (89.93%), Query Frame = 2

Query:   17 EEEGINDHNVVLKCAEIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLLAA 433
            EEEG+NDHNVV+KCAEIQ AISEGATSFLFT+Y YVG+FMV FA +IFLFLGS+EGFSTK QPCTY +   CKPAL TALFST SF+LG +TS++SGFLGMKIA YANARTTLEARK VGKAFITAFRSGAVMGFLL++
Sbjct:   58 EEEGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKGQPCTYSK-GTCKPALYTALFSTASFLLGAITSLVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGFLLSS 195

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BLAST of HO067341 vs. SwissProt
Match: HPPA_LEPIN (Pyrophosphate-energized proton pump OS=Leptospira interrogans GN=hppA PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 3.760e-16
Identity = 53/125 (42.40%), Postives = 68/125 (54.40%), Query Frame = 2

Query:   53 KCAEIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSV--EGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGF 421
            K  EI +AISEGA +FL   Y  + +F+   AVLI L L +   EGF+                     +++ I+FV G + S ISGF+GMKIA   N RT   A+ S+ KAF  AF SGAVMGF
Sbjct:   42 KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGSEGFND-------------------GIYTAIAFVSGALISCISGFIGMKIATAGNVRTAEAAKSSMAKAFRVAFDSGAVMGF 147

[Close]

BLAST of HO067341 vs. SwissProt
Match: HPPA_LEPIC (Pyrophosphate-energized proton pump OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni GN=hppA PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 3.760e-16
Identity = 53/125 (42.40%), Postives = 68/125 (54.40%), Query Frame = 2

Query:   53 KCAEIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSV--EGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGF 421
            K  EI +AISEGA +FL   Y  + +F+   AVLI L L +   EGF+                     +++ I+FV G + S ISGF+GMKIA   N RT   A+ S+ KAF  AF SGAVMGF
Sbjct:   42 KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGSEGFND-------------------GIYTAIAFVSGALISCISGFIGMKIATAGNVRTAEAAKSSMAKAFRVAFDSGAVMGF 147

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BLAST of HO067341 vs. SwissProt
Match: HPPA2_RHOPA (Pyrophosphate-energized proton pump OS=Rhodopseudomonas palustris GN=hppA PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.361e-10
Identity = 40/122 (32.79%), Postives = 54/122 (44.26%), Query Frame = 2

Query:   62 EIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLL 427
            EI  AI +GA ++L   Y  + I     A+LIF FLG +                           +   FVLG V S   GF+GM ++  AN RT   A K +G A   AF+ GA+ G L+
Sbjct:   40 EIAGAIQQGAAAYLARQYKTIAIVGAVLAILIFFFLGGL---------------------------TAAGFVLGAVLSGACGFIGMNVSVRANVRTAQAATKGIGPALDVAFKGGAITGMLV 134

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BLAST of HO067341 vs. SwissProt
Match: HPPA_CLOTE (Pyrophosphate-energized proton pump OS=Clostridium tetani GN=hppA PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 3.083e-10
Identity = 42/119 (35.29%), Postives = 57/119 (47.90%), Query Frame = 2

Query:   62 EIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMG 418
            EI   I +GA +FL T Y Y+  F+V   V++ +F+G               +T  C             F+LG + S+ +G+ GM +A  AN RT   AR S GKA   AF  GAVMG
Sbjct:   38 EIAGHIHDGAMAFLKTEYKYLTGFIVIVTVILAIFVGW--------------QTAAC-------------FILGAIFSIFAGYFGMNVATKANVRTAEAARHSQGKALNIAFSGGAVMG 129

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BLAST of HO067341 vs. SwissProt
Match: HPPA_BACTN (Pyrophosphate-energized proton pump OS=Bacteroides thetaiotaomicron GN=hppA PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 3.083e-10
Identity = 45/122 (36.89%), Postives = 60/122 (49.18%), Query Frame = 2

Query:   62 EIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLL 427
            +I  A+ +GA S+L   Y  VG     F  L+ LF     GF  +                   ++  I+F+ GG  S +SGFLGMK A YA+ART   AR S+      AFRSGAVMG ++
Sbjct:   39 KIAAAVRKGAMSYLKQQYKIVGC---VFLGLVILFSIMAYGFHVQN------------------VWVPIAFLTGGFFSGLSGFLGMKTATYASARTANAARNSLNSGLRIAFRSGAVMGLVV 139

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BLAST of HO067341 vs. SwissProt
Match: HPPA_XANAC (Pyrophosphate-energized proton pump OS=Xanthomonas axonopodis pv. citri GN=hppA PE=3 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.530e-9
Identity = 39/122 (31.97%), Postives = 56/122 (45.90%), Query Frame = 2

Query:   62 EIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLL 427
            EI  AI EGA ++L   Y  + +      VL+ LFL                           + ++ I F LG V S ++G++GM ++  AN RT   AR  +GKA   AFR GA+ G L+
Sbjct:   40 EIAAAIQEGARAYLNRQYLTISVAGAVLFVLVGLFL---------------------------SWYTAIGFALGAVLSGLAGYIGMNVSVRANVRTAEAARHGIGKAMDVAFRGGAITGMLV 134

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BLAST of HO067341 vs. SwissProt
Match: HPPA_XANCP (Pyrophosphate-energized proton pump OS=Xanthomonas campestris pv. campestris GN=hppA PE=3 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 2.610e-9
Identity = 39/122 (31.97%), Postives = 56/122 (45.90%), Query Frame = 2

Query:   62 EIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLL 427
            EI  AI EGA ++L   Y  + I  V   VL+ LFL                           + ++ I F +G V S ++G++GM ++  AN RT   AR  + KA   AFR GA+ G L+
Sbjct:   40 EIAAAIQEGARAYLNRQYLTISIAGVVLFVLVGLFL---------------------------SWYTAIGFAIGAVLSGLAGYIGMNVSVRANVRTAQAARHGISKAMDVAFRGGAITGMLV 134

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BLAST of HO067341 vs. TrEMBL
Match: B6DXD7_MEDTR (Vacuolar-type H-pyrophosphatase OS=Medicago truncatula GN=VP1 PE=2 SV=1)

HSP 1 Score: 255.758 bits (652), Expect = 1.050e-66
Identity = 127/139 (91.37%), Postives = 134/139 (96.40%), Query Frame = 2

Query:   17 EEEGINDHNVVLKCAEIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLLAA 433
            EEEGINDHNVVLKCAEIQNAISEG+TSFLFT+Y YVGIFMVAFA+LIFLFLGSVEGFST  QPCTYDETK+CKPALATALFSTI+F+LGG+TSVISGFLGMKIA YANARTTLEARK VGKAFITAFRSGAVMGFLLAA
Sbjct:   61 EEEGINDHNVVLKCAEIQNAISEGSTSFLFTMYKYVGIFMVAFAILIFLFLGSVEGFSTSHQPCTYDETKMCKPALATALFSTIAFILGGITSVISGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGFLLAA 199

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BLAST of HO067341 vs. TrEMBL
Match: Q43797_TOBAC (Inorganic pyrophosphatase OS=Nicotiana tabacum GN=ppa PE=2 SV=1)

HSP 1 Score: 246.899 bits (629), Expect = 4.878e-64
Identity = 123/139 (88.49%), Postives = 129/139 (92.81%), Query Frame = 2

Query:   17 EEEGINDHNVVLKCAEIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLLAA 433
            EEEGINDHNVV KCAEIQNAISEGATSFLFT Y YVG+FMVAFA+LIFLFLGSVEGFSTK QPCTYD TK CKPALATA+FST+SF+LG VTSV+SGFLGMKIA YANARTTLEARK VGKAFI AFRSGAVMGFLLAA
Sbjct:   61 EEEGINDHNVVQKCAEIQNAISEGATSFLFTEYQYVGVFMVAFAILIFLFLGSVEGFSTKNQPCTYDSTKTCKPALATAVFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFIVAFRSGAVMGFLLAA 199

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BLAST of HO067341 vs. TrEMBL
Match: A9PFH8_POPTR (Putative uncharacterized protein OS=Populus trichocarpa GN=POPTRDRAFT_578868 PE=2 SV=1)

HSP 1 Score: 246.899 bits (629), Expect = 4.878e-64
Identity = 124/139 (89.21%), Postives = 130/139 (93.53%), Query Frame = 2

Query:   17 EEEGINDHNVVLKCAEIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLLAA 433
            EEEG+NDHN+VLKCAEIQNAISEGATSFLFT Y YVGIFMVAFA+LIF+FLGSVEGFSTK QPCTYD  KLCKPALATA FSTI+FVLG VTSV+SGFLGMKIA YANARTTLEARK VGKAFITAFRSGAVMGFLLAA
Sbjct:   63 EEEGLNDHNIVLKCAEIQNAISEGATSFLFTEYQYVGIFMVAFAILIFVFLGSVEGFSTKSQPCTYDPLKLCKPALATAGFSTIAFVLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGFLLAA 201

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BLAST of HO067341 vs. TrEMBL
Match: D3THK8_LOTCO (Tonoplast proton pump OS=Lotus corniculatus GN=VP1 PE=2 SV=1)

HSP 1 Score: 245.743 bits (626), Expect = 1.087e-63
Identity = 124/139 (89.21%), Postives = 129/139 (92.81%), Query Frame = 2

Query:   17 EEEGINDHNVVLKCAEIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLLAA 433
            EEEGIND NVVLKCAEIQ AISEGATSFLFT Y YVGIFMV FA++IFLFLGSVEGFST  QPC YDETK+CKPALATALFSTISFVLGG+TSV+SGFLGMKIA YANARTTLEARK VGKAFITAFRSGAVMGFLLAA
Sbjct:   63 EEEGINDQNVVLKCAEIQQAISEGATSFLFTEYKYVGIFMVFFAIVIFLFLGSVEGFSTSHQPCKYDETKICKPALATALFSTISFVLGGITSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGFLLAA 201

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BLAST of HO067341 vs. TrEMBL
Match: B9SXN6_RICCO (Pyrophosphate-energized vacuolar membrane proton pump, putative OS=Ricinus communis GN=RCOM_0003350 PE=4 SV=1)

HSP 1 Score: 245.358 bits (625), Expect = 1.419e-63
Identity = 121/139 (87.05%), Postives = 130/139 (93.53%), Query Frame = 2

Query:   17 EEEGINDHNVVLKCAEIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLLAA 433
            EEEG+NDHNVVLKCA+IQNAISEGATSFLFT Y YVGIFMVAFA+LIF+FLGSVEGFSTK QPCTYD+ K+CKPALATA FST+SF+LG  TSV+SGFLGMKIA YANARTTLEARK VGKAFITAFRSGAVMGFLLAA
Sbjct:   62 EEEGVNDHNVVLKCADIQNAISEGATSFLFTEYQYVGIFMVAFAILIFVFLGSVEGFSTKSQPCTYDQFKMCKPALATAAFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGFLLAA 200

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BLAST of HO067341 vs. TrEMBL
Match: O22124_9FABA (Proton pyrophosphatase OS=Vigna radiata PE=2 SV=1)

HSP 1 Score: 244.973 bits (624), Expect = 1.854e-63
Identity = 122/139 (87.77%), Postives = 131/139 (94.24%), Query Frame = 2

Query:   17 EEEGINDHNVVLKCAEIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLLAA 433
            EEEGINDHNVV+KCAEIQNAISEGATSFLFT Y YVGIFMVAFA+LIFLFLGSVEGFST  Q C+YD+TK CKPALATA+FST+SF+LGGVTS++SGFLGMKIA YANARTTLEARK VGKAFITAFRSGAVMGFLLAA
Sbjct:   61 EEEGINDHNVVVKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAILIFLFLGSVEGFSTSPQACSYDKTKTCKPALATAIFSTVSFLLGGVTSLVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGFLLAA 199

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BLAST of HO067341 vs. TrEMBL
Match: Q8GT22_PYRCO (Vacuolar proton-inorganic pyrophosphatase OS=Pyrus communis GN=PVP3 PE=2 SV=1)

HSP 1 Score: 244.202 bits (622), Expect = 3.162e-63
Identity = 119/139 (85.61%), Postives = 130/139 (93.53%), Query Frame = 2

Query:   17 EEEGINDHNVVLKCAEIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLLAA 433
            EEEG+ND NVV+KCAEIQNAISEG+TSFLFT+Y YVG+FMV FA+LIFLFLGSVEGFSTK QPCTYD  K CKPALATA+FST++FVLGG+TSV+SGFLGMKIA YANARTTLEARK VGKAFITAFRSGAVMGFLLAA
Sbjct:   62 EEEGLNDQNVVVKCAEIQNAISEGSTSFLFTMYQYVGVFMVVFAILIFLFLGSVEGFSTKSQPCTYDAAKTCKPALATAIFSTVAFVLGGITSVLSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGFLLAA 200

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BLAST of HO067341 vs. TrEMBL
Match: Q197Z6_NICRU (Inorganic pyrophosphatase OS=Nicotiana rustica GN=AVP1 PE=2 SV=1)

HSP 1 Score: 243.432 bits (620), Expect = 5.394e-63
Identity = 121/139 (87.05%), Postives = 130/139 (93.53%), Query Frame = 2

Query:   17 EEEGINDHNVVLKCAEIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLLAA 433
            EEEGIND NVV+KCAEIQNAISEGATSFLFT Y YVGIFM+AFA+LIFLFLGSVEGFSTK QPCTY++ KLCKPALATA+FST+SF+LG VTSV+SGFLGMKIA YANARTTLEARK VGKAFI AFRSGAVMGFLLAA
Sbjct:   60 EEEGINDQNVVVKCAEIQNAISEGATSFLFTEYQYVGIFMIAFAILIFLFLGSVEGFSTKSQPCTYNKEKLCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFIVAFRSGAVMGFLLAA 198

[Close]

BLAST of HO067341 vs. TrEMBL
Match: B9N710_POPTR (Vacuolar H+-translocating inorganic pyrophosphatase OS=Populus trichocarpa GN=POPTRDRAFT_828371 PE=4 SV=1)

HSP 1 Score: 243.047 bits (619), Expect = 7.044e-63
Identity = 122/139 (87.77%), Postives = 129/139 (92.81%), Query Frame = 2

Query:   17 EEEGINDHNVVLKCAEIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLLAA 433
            EEEG+NDHNVVLKCAEIQNAISEGATSFLFT Y YVGIFMVAFA+LIF+FLGSVEGFSTK QPCTYD  K+CKPALATA FST++FVLG VTSV+SGFLGMKIA YANARTTLEARK VGKAFI AFRSGAVMGFLLAA
Sbjct:   63 EEEGLNDHNVVLKCAEIQNAISEGATSFLFTEYQYVGIFMVAFAILIFVFLGSVEGFSTKSQPCTYDLLKMCKPALATAGFSTVAFVLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFIIAFRSGAVMGFLLAA 201

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BLAST of HO067341 vs. TrEMBL
Match: O82680_CUCMO (Proton-translocating inorganic pyrophosphatase OS=Cucurbita moschata GN=PHP-1 PE=2 SV=1)

HSP 1 Score: 242.276 bits (617), Expect = 1.202e-62
Identity = 120/139 (86.33%), Postives = 130/139 (93.53%), Query Frame = 2

Query:   17 EEEGINDHNVVLKCAEIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLLAA 433
            EEEG+NDHNVV+KCAEIQ+AISEGATSFLFT Y YVGIFMV FAVLIF+FLGSVE FSTK QPCTYD+T+ CKPALATA+FST+SF+LG VTSV+SGFLGMKIA YANARTTLEARK VGKAFITAFRSGAVMGFLLAA
Sbjct:   63 EEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGFLLAA 201

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BLAST of HO067341 vs. TAIR peptide
Match: AT1G15690.2 (| Symbols: AVP1, ATAVP3, AVP-3, AtVHP1;1 | Inorganic H pyrophosphatase family protein | chr1:5399115-5402185 FORWARD LENGTH=642)

HSP 1 Score: 226.868 bits (577), Expect = 3.369e-60
Identity = 113/139 (81.29%), Postives = 123/139 (88.49%), Query Frame = 2

Query:   17 EEEGINDHNVVLKCAEIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLLAA 433
            EEEG+ND +VV KCAEIQ AISEGATSFLFT Y YVG+FM+ FA +IF+FLGSVEGFST  +PCTYD T+ CKPALATA FSTI+FVLG VTSV+SGFLGMKIA YANARTTLEARK VGKAFI AFRSGAVMGFLLAA
Sbjct:   65 EEEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDNKPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGKAFIVAFRSGAVMGFLLAA 203

[Close]

BLAST of HO067341 vs. TAIR peptide
Match: AT1G15690.1 (| Symbols: AVP1, ATAVP3, AVP-3, AtVHP1;1 | Inorganic H pyrophosphatase family protein | chr1:5399115-5402185 FORWARD LENGTH=770)

HSP 1 Score: 226.868 bits (577), Expect = 3.369e-60
Identity = 113/139 (81.29%), Postives = 123/139 (88.49%), Query Frame = 2

Query:   17 EEEGINDHNVVLKCAEIQNAISEGATSFLFTVYYYVGIFMVAFAVLIFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSGAVMGFLLAA 433
            EEEG+ND +VV KCAEIQ AISEGATSFLFT Y YVG+FM+ FA +IF+FLGSVEGFST  +PCTYD T+ CKPALATA FSTI+FVLG VTSV+SGFLGMKIA YANARTTLEARK VGKAFI AFRSGAVMGFLLAA
Sbjct:   65 EEEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDNKPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGKAFIVAFRSGAVMGFLLAA 203

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BLAST of HO067341 vs. TAIR peptide
Match: AT1G16780.1 (| Symbols: VHP2;2, AtVHP2;2 | Inorganic H pyrophosphatase family protein | chr1:5739468-5743818 REVERSE LENGTH=851)

HSP 1 Score: 49.6766 bits (117), Expect = 7.371e-7
Identity = 36/118 (30.51%), Postives = 62/118 (52.54%), Query Frame = 2

Query:   62 EIQNAISEGATSFLFTVYYYVG--IFMVAFAVL-IFLFLGSVEGFSTKEQPCTYDETKLCKPALATALFSTISFVLGGVTSVISGFLGMKIAAYANARTTLEARKSVGKAFITAFRSG 406
            +I +AI +GA  FL T Y  +    F++AF +L I+LF       + +++      T        +A  +  +F+LG + S I+G++GM ++  AN R +  AR+S  +A   A R+G
Sbjct:  150 QISDAIRDGAEGFLRTQYGTISKMAFLLAFVILCIYLF----RNLTPQQEASGLGRTM-------SAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAG 256

The following BLAST results are available for this feature:

BLAST of HO067341 vs. SwissProt
BLAST of HO067341 vs. TrEMBL
BLAST of HO067341 vs. TAIR peptide

BLAST of HO067341 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
AVP_PHAAU	9.758e-65	87.77	Pyrophosphate-energized vacuolar membrane proton p...	[more]
AVP1_ARATH	2.750e-59	81.29	Pyrophosphate-energized vacuolar membrane proton p...	[more]
AVP_HORVU	1.045e-58	80.58	Pyrophosphate-energized vacuolar membrane proton p...	[more]
HPPA_LEPIN	3.760e-16	42.40	Pyrophosphate-energized proton pump OS=Leptospira ...	[more]
HPPA_LEPIC	3.760e-16	42.40	Pyrophosphate-energized proton pump OS=Leptospira ...	[more]
HPPA2_RHOPA	2.361e-10	32.79	Pyrophosphate-energized proton pump OS=Rhodopseudo...	[more]
HPPA_CLOTE	3.083e-10	35.29	Pyrophosphate-energized proton pump OS=Clostridium...	[more]
HPPA_BACTN	3.083e-10	36.89	Pyrophosphate-energized proton pump OS=Bacteroides...	[more]
HPPA_XANAC	1.530e-9	31.97	Pyrophosphate-energized proton pump OS=Xanthomonas...	[more]
HPPA_XANCP	2.610e-9	31.97	Pyrophosphate-energized proton pump OS=Xanthomonas...	[more]

BLAST of HO067341 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B6DXD7_MEDTR	1.050e-66	91.37	Vacuolar-type H-pyrophosphatase OS=Medicago trunca...	[more]
Q43797_TOBAC	4.878e-64	88.49	Inorganic pyrophosphatase OS=Nicotiana tabacum GN=...	[more]
A9PFH8_POPTR	4.878e-64	89.21	Putative uncharacterized protein OS=Populus tricho...	[more]
D3THK8_LOTCO	1.087e-63	89.21	Tonoplast proton pump OS=Lotus corniculatus GN=VP1...	[more]
B9SXN6_RICCO	1.419e-63	87.05	Pyrophosphate-energized vacuolar membrane proton p...	[more]
O22124_9FABA	1.854e-63	87.77	Proton pyrophosphatase OS=Vigna radiata PE=2 SV=1	[more]
Q8GT22_PYRCO	3.162e-63	85.61	Vacuolar proton-inorganic pyrophosphatase OS=Pyrus...	[more]
Q197Z6_NICRU	5.394e-63	87.05	Inorganic pyrophosphatase OS=Nicotiana rustica GN=...	[more]
B9N710_POPTR	7.044e-63	87.77	Vacuolar H+-translocating inorganic pyrophosphatas...	[more]
O82680_CUCMO	1.202e-62	86.33	Proton-translocating inorganic pyrophosphatase OS=...	[more]

BLAST of HO067341 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT1G15690.2	3.369e-60	81.29	\| Symbols: AVP1, ATAVP3, AVP-3, AtVHP1;1 \| Inorgan...	[more]
AT1G15690.1	3.369e-60	81.29	\| Symbols: AVP1, ATAVP3, AVP-3, AtVHP1;1 \| Inorgan...	[more]
AT1G16780.1	7.371e-7	30.51	\| Symbols: VHP2;2, AtVHP2;2 \| Inorganic H pyrophos...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004131	Pyrophosphate-energised proton pump	PFAM	PF03030	H_PPase	coord: 18..143 score: 2.9

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO067341 ID=HO067341; Name=HO067341; organism=Cicer arietinum; type=EST; length=435bp
CGGGCAGGTACGCGGGGAAGAAGAGGGTATTAACGACCACAACGTCGTTC
TTAAATGTGCTGAAATTCAGAACGCTATTTCTGAAGGTGCAACCTCTTTC
CTTTTCACCGTGTATTATTATGTAGGGATTTTCATGGTGGCTTTTGCTGT
CTTGATATTCCTCTTCCTTGGATCTGTGGAAGGATTCAGCACAAAGGAAC
AGCCCTGCACTTATGATGAAACTAAACTCTGCAAGCCAGCTCTTGCTACA
GCCCTTTTCAGCACCATCTCATTTGTGCTTGGTGGCGTCACATCAGTTAT
TTCTGGTTTCCTTGGAATGAAAATTGCAGCTTATGCCAATGCAAGAACCA
CCCTGGAGGCAAGAAAGAGTGTTGGGAAGGCTTTCATTACGGCATTTAGA
TCAGGTGCAGTTATGGGATTTCTTCTTGCTGCAAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004427	inorganic diphosphate phosphatase activity
GO:0009678	diphosphate hydrolysis-driven proton transmembrane transporter activity

Vocabulary: **Biological Process**
Term	Definition
GO:0015992	proton transport

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR004131	PPase-energised_H-pump

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