AJ006048

EST Overview
NameAJ006048
Unique NameAJ006048
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length747
Libraries
Library NameType
LIBEST_001193 chickpea mRNA CAN-5cdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
KEGG analysis for Cicer arietinum unigene v12011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
InterProScan analysis for Cicer arietinum unigene v12010-12-29
Homology
[Close]
BLAST of AJ006048 vs. TrEMBL
Match: B7FMP3_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 2.956e-31
Identity = 79/145 (54.48%), Postives = 94/145 (64.83%), Query Frame = 3 
Query:  111 SNATEPESNSNDDQHEQPQPPPTVTDN-----QXXXXXXXXKPSMKFRNYVPHDKHLQEGKLAPALLPKFXXXXXXXXXXXXTQPKEDRFLILLLRNRIGICVEMCRRSLISLEKATQKALYQLMVEQEKQNQLAEGDDTNGVKD 530
            SN++EPE N+ D+  EQPQPPPT +++     +        K SMKFRNYVPHDK+LQEGKLAPA+LPKF             +PKED FL +  +          ++ L  LEK TQKALYQLMVEQEKQNQLAEGDDTNG KD
Sbjct:   29 SNSSEPEPNNQDE--EQPQPPPTSSEDDDEQREEKDGEGEEKLSMKFRNYVPHDKNLQEGKLAPAVLPKFEDPVSVPEPKE--KPKEDPFLNIAPKKPNWDLRRDVQKKLDKLEKRTQKALYQLMVEQEKQNQLAEGDDTNGTKD 169

HSP 2 Score: 23.8682 bits (50), Expect = 2.956e-31
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 2 
Query:   26 MGSEEESIEQAV 61
            MG+E+ESIEQAV
Sbjct:    1 MGTEDESIEQAV 12
[Close]
BLAST of AJ006048 vs. TrEMBL
Match: C6SY40_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 6.441e-24
Identity = 69/138 (50.00%), Postives = 79/138 (57.25%), Query Frame = 3 
Query:  111 SNATEPESNSN-DDQHEQPQPPPTVTDNQXXXXXXXXKPSMKFRNYVPHDKHLQEGKLAPALLPKFXXXXXXXXXXXXTQPKEDRFLILLLRNRIGICVEMCRRSLISLEKATQKALYQLMVEQEKQNQLAEGDDTNG 521
            S+  EPE N N DD +E+ Q P                  MKFRNYVPHDK+LQEGKLAPA+LPKF             + KED FL +  +          ++ L  LEK TQKALYQLMVEQEKQNQL EGDDTNG
Sbjct:   29 SSVPEPEPNGNKDDDNEEDQEPQ----------------EMKFRNYVPHDKNLQEGKLAPAVLPKFEDPVDDVPPPKP-EAKEDPFLNIAPKKPNWDLRRDVQKKLDKLEKRTQKALYQLMVEQEKQNQLTEGDDTNG 149

HSP 2 Score: 23.8682 bits (50), Expect = 6.441e-24
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 2 
Query:   26 MGSEEESIEQAV 61
            MGSEE+SIEQ+V
Sbjct:    1 MGSEEDSIEQSV 12
[Close]
BLAST of AJ006048 vs. TrEMBL
Match: C6T3P2_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 1.420e-23
Identity = 68/141 (48.23%), Postives = 81/141 (57.45%), Query Frame = 3 
Query:  111 SNATEPE-SNSNDDQHEQPQPPPTVTDNQXXXXXXXXKPSMKFRNYVPHDKHLQEGKLAPALLPKFXXXXXXXXXXXXTQPKEDRFLILLLRNRIGICVEMCRRSLISLEKATQKALYQLMVEQEKQNQLAEGDDTNGVKD 530
            S+A EPE  ++NDD +E+ Q P                  MKFRNYVPHDK+LQEGKLAPA+LPKF             +  ED FL +  +          ++ L  LEK TQKALYQLMVEQEKQNQL EGDDTNG  +
Sbjct:   29 SSAAEPEPKDNNDDDNEEDQEPQ----------------EMKFRNYVPHDKNLQEGKLAPAVLPKFEDPVDAVPPPEP-EAAEDPFLNIAPKKPNWDLRRDVQKKLDKLEKRTQKALYQLMVEQEKQNQLTEGDDTNGTAE 152

HSP 2 Score: 23.8682 bits (50), Expect = 1.420e-23
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 2 
Query:   26 MGSEEESIEQAV 61
            MGSEE+SIEQ+V
Sbjct:    1 MGSEEDSIEQSV 12
[Close]
BLAST of AJ006048 vs. TrEMBL
Match: D7T7D9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_39.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00024249001 PE=4 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.414e-15
Identity = 50/120 (41.67%), Postives = 69/120 (57.50%), Query Frame = 3 
Query:  144 DDQHEQPQPPPTVTDNQXXXXXXXXKPSMKFRNYVPHDKHLQEGKLAPALLPKFXXXXXXXXXXXXTQPKEDRFLILLLRNRIGICVEMCRRSLISLEKATQKALYQLMVEQEKQNQLAE 503
            +DQ +     PT+ ++          PSMKFRNY+PHDK LQEGKLAP +LPKF            ++ KED F+ +  +          ++ L  LE+ TQKA+++LM EQEK+NQLAE
Sbjct:   33 EDQDKPDDANPTMEEDN---------PSMKFRNYLPHDKELQEGKLAPPVLPKF--EDPVAAAPPPSEEKEDPFVNIAPKKPNWDLRRDVQKKLDKLERRTQKAIFKLMEEQEKENQLAE 141

HSP 2 Score: 23.0978 bits (48), Expect = 1.414e-15
Identity = 10/11 (90.91%), Postives = 10/11 (90.91%), Query Frame = 2 
Query:   26 MGSEEESIEQA 58
            MG EEESIEQA
Sbjct:    1 MGEEEESIEQA 11
[Close]
BLAST of AJ006048 vs. TrEMBL
Match: B9H295_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_817783 PE=4 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 1.247e-13
Identity = 47/103 (45.63%), Postives = 60/103 (58.25%), Query Frame = 3 
Query:  222 PSMKFRNYVPHDKHLQEGKLAPALLPKFXXXXXXXXXXXXTQPKEDRFLILLLRNRIGICVEMCRRSLISLEKATQKALYQLMVEQEKQNQLAEGDDTNGVKD 530
            PSMKFRNYVP DK LQEGKLAP +LPKF            ++ +ED FL +  +          +  L  LE+ TQKA+Y+LM EQEK+ QL+E      V+D
Sbjct:   54 PSMKFRNYVPQDKELQEGKLAPPVLPKF--EDPVAAAPPPSEKEEDPFLNIAPKKPNWDLRRDVQNKLDKLERRTQKAIYKLMEEQEKEKQLSENGGNGVVED 154
[Close]
BLAST of AJ006048 vs. TrEMBL
Match: Q9MAB2_ARATH (At3g05070 OS=Arabidopsis thaliana GN=At3g05070 PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 5.794e-11
Identity = 41/96 (42.71%), Postives = 56/96 (58.33%), Query Frame = 3 
Query:  222 PSMKFRNYVPHDKHLQEGKLAPALLPKFXXXXXXXXXXXXTQPKEDRFLILLLRNRIGICVEMCRRSLISLEKATQKALYQLMVEQEKQNQLAEGD 509
            P+MKFRNYVP  K LQ+GKLAP  LPKF             + KED F+ +  +          ++ L  LE+ TQKA+++LM EQEK+ ++AE D
Sbjct:   45 PAMKFRNYVPQAKELQDGKLAPPELPKF--EDPIVALPPAVEKKEDPFVNIAPKKPNWDLRRDVQKKLDKLERRTQKAMHKLMEEQEKEREMAEAD 138
[Close]
BLAST of AJ006048 vs. TrEMBL
Match: D7L3Q1_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_477790 PE=4 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.291e-10
Identity = 41/96 (42.71%), Postives = 54/96 (56.25%), Query Frame = 3 
Query:  222 PSMKFRNYVPHDKHLQEGKLAPALLPKFXXXXXXXXXXXXTQPKEDRFLILLLRNRIGICVEMCRRSLISLEKATQKALYQLMVEQEKQNQLAEGD 509
            P+MKFRNYVP  K LQ+GKLAP  LPKF             + KED F+ +  +          ++ L  LE+ TQKA+Y+LM E EK+ + AE D
Sbjct:   45 PAMKFRNYVPQAKELQDGKLAPPELPKF--EDPILALPPAVEKKEDPFVNIAPKKPNWDLRRDVQKKLDKLERRTQKAMYKLMEEHEKERETAEAD 138
[Close]
BLAST of AJ006048 vs. TrEMBL
Match: B9S0R3_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1222110 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 3.756e-10
Identity = 40/83 (48.19%), Postives = 50/83 (60.24%), Query Frame = 3 
Query:  222 PSMKFRNYVPHDKHLQEGKLAPALLPKFXXXXXXXXXXXXTQPKEDRFLILLLRNRIGICVEMCRRSLISLEKATQKALYQLM 470
            PSMKFRNYVP DK LQEGKLAPA+LPKF            ++ KED FL +  +          ++ L  LE+ TQKA+Y+LM
Sbjct:   65 PSMKFRNYVPQDKELQEGKLAPAVLPKF--EDPVAAAPPPSEKKEDPFLNIAPKKPNWDLRRDVQKKLDKLERRTQKAIYKLM 145
[Close]
BLAST of AJ006048 vs. TrEMBL
Match: Q5Z414_ORYSJ (Os06g0730800 protein OS=Oryza sativa subsp. japonica GN=B1206D04.12 PE=2 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 7.083e-9
Identity = 42/113 (37.17%), Postives = 60/113 (53.10%), Query Frame = 3 
Query:  183 TDNQXXXXXXXXKPSMKFRNYVPHDKHLQEGKLAPALLPKFXXXXXXXXXXXXTQPK--EDRFLILLLRNRIGICVEMCRRSLISLEKATQKALYQLMVEQEKQNQ-LAEGDD 512
            TD           P+MKFRNY+PHD+ L+ GK+AP  LPKF            T+PK  E+ F  +  +N         ++ +  LEK TQKAL ++ +EQ+K+ + L EG D
Sbjct:   70 TDESEEVQNDGDMPAMKFRNYLPHDEQLRGGKVAPVSLPKF----EDPISAETTEPKQVENPFGNIAPKNPNWDLKRDVQKRMDKLEKRTQKALAEIALEQQKEKEALGEGSD 178
[Close]
BLAST of AJ006048 vs. TrEMBL
Match: B8B2X7_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_24550 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 7.083e-9
Identity = 42/113 (37.17%), Postives = 60/113 (53.10%), Query Frame = 3 
Query:  183 TDNQXXXXXXXXKPSMKFRNYVPHDKHLQEGKLAPALLPKFXXXXXXXXXXXXTQPK--EDRFLILLLRNRIGICVEMCRRSLISLEKATQKALYQLMVEQEKQNQ-LAEGDD 512
            TD           P+MKFRNY+PHD+ L+ GK+AP  LPKF            T+PK  E+ F  +  +N         ++ +  LEK TQKAL ++ +EQ+K+ + L EG D
Sbjct:   70 TDESEEVQNDGDMPAMKFRNYLPHDEQLRGGKVAPVSLPKF----EDPISAETTEPKQVENPFGNIAPKNPNWDLKRDVQKRMDKLEKRTQKALAEIALEQQKEKEALGEGSD 178
[Close]
BLAST of AJ006048 vs. TAIR peptide
Match: AT3G05070.1 (| Symbols: | CONTAINS InterPro DOMAIN/s: mRNA splicing factor, Cwf18 (InterPro:IPR013169); Has 292 Blast hits to 292 proteins in 153 species: Archae - 0; Bacteria - 0; Metazoa - 118; Fungi - 83; Plants - 38; Viruses - 11; Other Eukaryotes - 42 (source: NCBI BLink). | chr3:1416480-1417533 FORWARD LENGTH=144)

HSP 1 Score: 72.0182 bits (175), Expect = 3.839e-13
Identity = 41/96 (42.71%), Postives = 56/96 (58.33%), Query Frame = 3 
Query:  222 PSMKFRNYVPHDKHLQEGKLAPALLPKFXXXXXXXXXXXXTQPKEDRFLILLLRNRIGICVEMCRRSLISLEKATQKALYQLMVEQEKQNQLAEGD 509
            P+MKFRNYVP  K LQ+GKLAP  LPKF             + KED F+ +  +          ++ L  LE+ TQKA+++LM EQEK+ ++AE D
Sbjct:   45 PAMKFRNYVPQAKELQDGKLAPPELPKF--EDPIVALPPAVEKKEDPFVNIAPKKPNWDLRRDVQKKLDKLERRTQKAMHKLMEEQEKEREMAEAD 138
The following BLAST results are available for this feature:
BLAST of AJ006048 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
B7FMP3_MEDTR2.956e-3154.48Putative uncharacterized protein OS=Medicago trunc... [more]
C6SY40_SOYBN6.441e-2450.00Putative uncharacterized protein OS=Glycine max PE... [more]
C6T3P2_SOYBN1.420e-2348.23Putative uncharacterized protein OS=Glycine max PE... [more]
D7T7D9_VITVI1.414e-1541.67Whole genome shotgun sequence of line PN40024, sca... [more]
B9H295_POPTR1.247e-1345.63Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
Q9MAB2_ARATH5.794e-1142.71At3g05070 OS=Arabidopsis thaliana GN=At3g05070 PE=... [more]
D7L3Q1_ARALY1.291e-1042.71Putative uncharacterized protein OS=Arabidopsis ly... [more]
B9S0R3_RICCO3.756e-1048.19Putative uncharacterized protein OS=Ricinus commun... [more]
Q5Z414_ORYSJ7.083e-937.17Os06g0730800 protein OS=Oryza sativa subsp. japoni... [more]
B8B2X7_ORYSI7.083e-937.17Putative uncharacterized protein OS=Oryza sativa s... [more]
back to top
BLAST of AJ006048 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT3G05070.13.839e-1342.71| Symbols: | CONTAINS InterPro DOMAIN/s: mRNA spl... [more]
back to top
InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 12..24
score: NAcoord: 29..53
score: NAcoord: 54..62
score
NoneNo IPR availableSEGsegsegcoord: 29..36
score: NAcoord: 66..77
score
NoneNo IPR availableSEGsegsegcoord: 20..36
score
NoneNo IPR availableSEGsegsegcoord: 3..23
score: NAcoord: 49..58
score: NAcoord: 64..98
score
IPR013169mRNA splicing factor, Cwf18PFAMPF08315cwf18coord: 4..26
score: 4.2
IPR013169mRNA splicing factor, Cwf18PFAMPF08315cwf18coord: 20..82
score: 1.7

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>AJ006048 ID=AJ006048; Name=AJ006048; organism=Cicer arietinum; type=EST; length=747bp
AACAATCAGAGCTACAGATACAAAGATGGGTAGCGAAGAGGAATCGATCG
AGCAAGCCGTTGCTTCACGTCGAGAAAGACTCCTAGCTCTAAGAGCCGCT
CAAGAATTAATCCAATGCCACCGAACCCGAATCTAACAGCAACGACGATC
AACACGAACAACCACAACCACCACCAACTGTTACTGATAACCAAGAAGAA
GATGATGAAGAAGAGGAAAAACCAAGCATGAAGTTCCGTAACTATGTTCC
TCACGATAAACATCTTCAAGAAGGAAAGCTTGCACCTGCTTTGCTTCCTA
AATTCGAAGATCCTGTTTCTGTTCCTGAGCCTGAACCTGAAACTCAACCT
AAGGAGGATCGTTTCTTAATATTGCTCCTAAGAAACCGAATTGGGATTTG
CGTAGAGATGTGCAGAAGAAGCTTGATAAGTCTCGAGAAAGCAACTCAGA
AGGCTCTCTATCAACTCATGGTGGAACAAGAGAAGCAAAATCAGTTGGCC
GAAGGAGATGATACAAATGGCGTCAAAGATTAGGATCATATCTGAATGTT
CTCATATAGAGATCAAATTCTCGCAATCCAAAAGCAAAACGGGTGTTTAA
ATTGTCATATATAACATTGTCCAAGTTTCAAATATCGGATATGTAAAGAT
TTTGGAGTTATGAAGAAATTGTTTGTTTTGAAGTTATGTCAAAGTGAGAC
GAAGAAATGTATGCTTAAAGCTTAATTAAGAGGTTAATATCTTTCTG
back to top
Annotated Terms
The following terms have been associated with this EST:
Vocabulary: INTERPRO
TermDefinition
IPR013169mRNA_splic_Cwf18-like