CK148741

EST Overview
NameCK148741
Unique NameCK148741
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length301
Libraries
Library NameType
LIBEST_014674 Suppressive subtractive hybridization librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
InterProScan analysis for Cicer arietinum unigene v12010-12-29
Homology
[Close]
BLAST of CK148741 vs. TrEMBL
Match: A9NWM6_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 7.020e-10
Identity = 29/59 (49.15%), Postives = 40/59 (67.80%), Query Frame = 2 
Query:   50 IKDIDELPKNPANYTALTPVWFLQSSELKFTQPESPSSMDPSFTWHHTYHRCRDSSALL 226
            +KDID+LPK PANYTALTP+WF+Q + L + + +S       +TW  TY RCR  ++ L
Sbjct:    6 VKDIDDLPKCPANYTALTPLWFIQRAALVYPKRKSVIHGARQYTWDQTYERCRRLASAL 64

HSP 2 Score: 21.557 bits (44), Expect = 7.020e-10
Identity = 9/12 (75.00%), Postives = 9/12 (75.00%), Query Frame = 3 
Query:  258 VCAPNIPDLYEA 293
            V APNIP  YEA
Sbjct:   77 VLAPNIPAAYEA 88
[Close]
BLAST of CK148741 vs. TrEMBL
Match: D7SY51_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_77.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00035030001 PE=4 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 4.361e-9
Identity = 28/63 (44.44%), Postives = 39/63 (61.90%), Query Frame = 2 
Query:   53 KDIDELPKNPANYTALTPVWFLQSSELKFTQPESPSSMDPSFTWHHTYHRCRDSSALLPFVSV 241
            +DID+LPKN ANYTALTP+WFL+ + +     +S       +TW  TY RCR  ++ L   S+
Sbjct:    5 RDIDDLPKNAANYTALTPLWFLERAAVVHPNRKSVIHGSLQYTWLQTYQRCRRLASALSKYSI 67

HSP 2 Score: 23.8682 bits (50), Expect = 4.361e-9
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:  255 AVCAPNIPDLYEA 293
            A+ APNIP +YEA
Sbjct:   74 AIIAPNIPAMYEA 86
[Close]
BLAST of CK148741 vs. TrEMBL
Match: B6SS27_MAIZE (AMP-binding protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 7.314e-9
Identity = 29/63 (46.03%), Postives = 39/63 (61.90%), Query Frame = 2 
Query:   53 KDIDELPKNPANYTALTPVWFLQSSELKFTQPESPSSMDPSFTWHHTYHRCRDSSALLPFVSV 241
            +DID+LP+N ANYTALTP+WFL+ + L      S       +TW  TY RCR  ++ L  +SV
Sbjct:   17 RDIDDLPRNDANYTALTPLWFLERAALAHPGRASVVHGPVRYTWADTYRRCRRLASALARLSV 79

HSP 2 Score: 23.483 bits (49), Expect = 7.314e-9
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:  255 AVCAPNIPDLYEA 293
            AV APN+P +YEA
Sbjct:   86 AVIAPNVPAVYEA 98
[Close]
BLAST of CK148741 vs. TrEMBL
Match: D7L689_ARALY (Acyl-activating enzyme 7 OS=Arabidopsis lyrata subsp. lyrata GN=AAE7 PE=4 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 7.318e-9
Identity = 27/58 (46.55%), Postives = 36/58 (62.07%), Query Frame = 2 
Query:   53 KDIDELPKNPANYTALTPVWFLQSSELKFTQPESPSSMDPSFTWHHTYHRCRDSSALL 226
            +DID+LPK PANYTALTP+WFL  + +     +S       +TW  TY RCR  ++ L
Sbjct:   10 RDIDDLPKIPANYTALTPLWFLDRAAMVHPTRKSVIHGSREYTWRQTYDRCRRLASAL 67

HSP 2 Score: 23.8682 bits (50), Expect = 7.318e-9
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:  255 AVCAPNIPDLYEA 293
            A+ APNIP +YEA
Sbjct:   79 AIIAPNIPAMYEA 91
[Close]
BLAST of CK148741 vs. TrEMBL
Match: Q9LSQ0_ARATH (AMP-binding protein OS=Arabidopsis thaliana PE=4 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 9.493e-9
Identity = 27/58 (46.55%), Postives = 36/58 (62.07%), Query Frame = 2 
Query:   53 KDIDELPKNPANYTALTPVWFLQSSELKFTQPESPSSMDPSFTWHHTYHRCRDSSALL 226
            +DID+LPK PANYTALTP+WFL  + +     +S       +TW  TY RCR  ++ L
Sbjct:    7 RDIDDLPKIPANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASAL 64

HSP 2 Score: 23.8682 bits (50), Expect = 9.493e-9
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:  255 AVCAPNIPDLYEA 293
            A+ APNIP +YEA
Sbjct:   78 AIIAPNIPAMYEA 90
[Close]
BLAST of CK148741 vs. TrEMBL
Match: Q8VZF1_ARATH (AT3g16910/K14A17_3 OS=Arabidopsis thaliana GN=AMPBP7 PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 9.494e-9
Identity = 27/58 (46.55%), Postives = 36/58 (62.07%), Query Frame = 2 
Query:   53 KDIDELPKNPANYTALTPVWFLQSSELKFTQPESPSSMDPSFTWHHTYHRCRDSSALL 226
            +DID+LPK PANYTALTP+WFL  + +     +S       +TW  TY RCR  ++ L
Sbjct:    7 RDIDDLPKIPANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASAL 64

HSP 2 Score: 23.8682 bits (50), Expect = 9.494e-9
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:  255 AVCAPNIPDLYEA 293
            A+ APNIP +YEA
Sbjct:   76 AIIAPNIPAMYEA 88
[Close]
BLAST of CK148741 vs. TrEMBL
Match: B9HIR3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_564415 PE=4 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 9.496e-9
Identity = 28/60 (46.67%), Postives = 37/60 (61.67%), Query Frame = 2 
Query:   47 RIKDIDELPKNPANYTALTPVWFLQSSELKFTQPESPSSMDPSFTWHHTYHR-CRDSSAL 223
            + +DID+LPKNPANY +LTP+WFL+ +        S       +TW  TY R CR +SAL
Sbjct:    3 KTRDIDDLPKNPANYMSLTPLWFLERAATVHPTRTSIVHESVQYTWQETYQRCCRFASAL 62

HSP 2 Score: 25.0238 bits (53), Expect = 9.496e-9
Identity = 12/22 (54.55%), Postives = 14/22 (63.64%), Query Frame = 3 
Query:  228 RSYRSRKHCAVCAPNIPDLYEA 293
            RS    +  AV APN+P LYEA
Sbjct:   65 RSLGLGRTVAVIAPNVPALYEA 86
[Close]
BLAST of CK148741 vs. TrEMBL
Match: B6SYD7_MAIZE (AMP-binding protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.232e-8
Identity = 29/63 (46.03%), Postives = 38/63 (60.32%), Query Frame = 2 
Query:   53 KDIDELPKNPANYTALTPVWFLQSSELKFTQPESPSSMDPSFTWHHTYHRCRDSSALLPFVSV 241
            +DID+LP+N ANYTALTP+WFL+ + L      S       +TW  TY RCR  ++ L   SV
Sbjct:    5 RDIDDLPRNDANYTALTPLWFLERAALTHPARASVVHGPVRYTWADTYSRCRRLASALARRSV 67

HSP 2 Score: 24.2534 bits (51), Expect = 1.232e-8
Identity = 10/13 (76.92%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:  255 AVCAPNIPDLYEA 293
            AV APNIP +YEA
Sbjct:   74 AVIAPNIPAIYEA 86
[Close]
BLAST of CK148741 vs. TrEMBL
Match: B4FAE2_MAIZE (AMP-binding protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.232e-8
Identity = 29/63 (46.03%), Postives = 38/63 (60.32%), Query Frame = 2 
Query:   53 KDIDELPKNPANYTALTPVWFLQSSELKFTQPESPSSMDPSFTWHHTYHRCRDSSALLPFVSV 241
            +DID+LP+N ANYTALTP+WFL+ + L      S       +TW  TY RCR  ++ L   SV
Sbjct:    5 RDIDDLPRNDANYTALTPLWFLERAALTHPARASVVHGPVRYTWADTYSRCRRLASALARRSV 67

HSP 2 Score: 24.2534 bits (51), Expect = 1.232e-8
Identity = 10/13 (76.92%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:  255 AVCAPNIPDLYEA 293
            AV APNIP +YEA
Sbjct:   74 AVIAPNIPAIYEA 86
[Close]
BLAST of CK148741 vs. TrEMBL
Match: C5WMI4_SORBI (Putative uncharacterized protein Sb01g037610 OS=Sorghum bicolor GN=Sb01g037610 PE=4 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 1.598e-8
Identity = 29/63 (46.03%), Postives = 38/63 (60.32%), Query Frame = 2 
Query:   53 KDIDELPKNPANYTALTPVWFLQSSELKFTQPESPSSMDPSFTWHHTYHRCRDSSALLPFVSV 241
            +DID+LP+N ANYTALTP+WFL+ + L      S       +TW  TY RCR  ++ L   SV
Sbjct:    5 RDIDDLPRNDANYTALTPLWFLERAALTHPARASVVHGPVRYTWADTYTRCRRLASALARRSV 67

HSP 2 Score: 24.2534 bits (51), Expect = 1.598e-8
Identity = 10/13 (76.92%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:  255 AVCAPNIPDLYEA 293
            AV APNIP +YEA
Sbjct:   74 AVIAPNIPAIYEA 86
[Close]
BLAST of CK148741 vs. TAIR peptide
Match: AT3G16910.1 (| Symbols: AAE7, ACN1 | acyl-activating enzyme 7 | chr3:5773231-5775411 REVERSE LENGTH=569)

HSP 1 Score: 59.3066 bits (142), Expect = 3.622e-11
Identity = 27/58 (46.55%), Postives = 36/58 (62.07%), Query Frame = 2 
Query:   53 KDIDELPKNPANYTALTPVWFLQSSELKFTQPESPSSMDPSFTWHHTYHRCRDSSALL 226
            +DID+LPK PANYTALTP+WFL  + +     +S       +TW  TY RCR  ++ L
Sbjct:    7 RDIDDLPKIPANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASAL 64

HSP 2 Score: 23.8682 bits (50), Expect = 3.622e-11
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:  255 AVCAPNIPDLYEA 293
            A+ APNIP +YEA
Sbjct:   76 AIIAPNIPAMYEA 88
The following BLAST results are available for this feature:
BLAST of CK148741 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
A9NWM6_PICSI7.020e-1049.15Putative uncharacterized protein OS=Picea sitchens... [more]
D7SY51_VITVI4.361e-944.44Whole genome shotgun sequence of line PN40024, sca... [more]
B6SS27_MAIZE7.314e-946.03AMP-binding protein OS=Zea mays PE=2 SV=1[more]
D7L689_ARALY7.318e-946.55Acyl-activating enzyme 7 OS=Arabidopsis lyrata sub... [more]
Q9LSQ0_ARATH9.493e-946.55AMP-binding protein OS=Arabidopsis thaliana PE=4 S... [more]
Q8VZF1_ARATH9.494e-946.55AT3g16910/K14A17_3 OS=Arabidopsis thaliana GN=AMPB... [more]
B9HIR3_POPTR9.496e-946.67Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B6SYD7_MAIZE1.232e-846.03AMP-binding protein OS=Zea mays PE=2 SV=1[more]
B4FAE2_MAIZE1.232e-846.03AMP-binding protein OS=Zea mays PE=2 SV=1[more]
C5WMI4_SORBI1.598e-846.03Putative uncharacterized protein Sb01g037610 OS=So... [more]
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BLAST of CK148741 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT3G16910.13.622e-1146.55| Symbols: AAE7, ACN1 | acyl-activating enzyme 7 |... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR11968ATP-DEPENDENT AMP-BINDING ENZYME FAMILY MEMBERcoord: 8..55
score: 1.9
NoneNo IPR availablePANTHERPTHR11968:SF26ADENOSINE MONOPHOSPHATE BINDING PROTEINcoord: 8..55
score: 1.9

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>CK148741 ID=CK148741; Name=CK148741; organism=Cicer arietinum; type=EST; length=301bp
AGATCTCTAGAGCTATAGGCATATCATCGCGCCATAGCTGTGACCTCGGA
TTAAGGACATAGACGAATTACCGAAGAACCCTGCCAACTACACAGCTTTA
ACGCCGGTATGGTTCTTACAAAGTAGCGAGCTCAAGTTCACCCAACCAGA
ATCTCCATCATCCATGGATCCGTCATTTACGTGGCATCACACTTACCACC
GTTGCCGTGATTCCTCCGCTCTTTTACCGTTCGTATCGGTCCCGGAAACA
CTGTGCTGTATGTGCACCCAATATTCCAGATCTTTATGAAGCTATTTGGG
A
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