GR916794

EST Overview


Name	GR916794
Unique Name	GR916794
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	294

Libraries


Library Name	Type
LIBEST_024969 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR916794 vs. SwissProt
Match: ALIS3_ARATH (ALA-interacting subunit 3 OS=Arabidopsis thaliana GN=ALIS3 PE=1 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.393e-20
Identity = 43/49 (87.76%), Postives = 47/49 (95.92%), Query Frame = -1

Query:  124 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGG 270
            GKNDFLGIAYLTVGG+CF L+LAFTI+Y VKPR+LGDPSYLSWNRNPGG
Sbjct:  300 GKNDFLGIAYLTVGGICFILALAFTIMYLVKPRRLGDPSYLSWNRNPGG 348

[Close]

BLAST of GR916794 vs. SwissProt
Match: ALIS1_ARATH (ALA-interacting subunit 1 OS=Arabidopsis thaliana GN=ALIS1 PE=1 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 5.855e-19
Identity = 40/49 (81.63%), Postives = 46/49 (93.88%), Query Frame = -1

Query:  124 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGG 270
            GKNDFLGIAYLTVGG+CF L+LAFT++Y VKPR+LGDP+YLSWNR PGG
Sbjct:  301 GKNDFLGIAYLTVGGICFVLALAFTVMYLVKPRRLGDPTYLSWNRIPGG 349

[Close]

BLAST of GR916794 vs. SwissProt
Match: ALIS5_ARATH (ALA-interacting subunit 5 OS=Arabidopsis thaliana GN=ALIS5 PE=1 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 5.480e-17
Identity = 36/49 (73.47%), Postives = 43/49 (87.76%), Query Frame = -1

Query:  124 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGG 270
            G+NDFLGIAYLTVG +C FL++ F ++Y VKPRQLGDPSYLSWNR+ GG
Sbjct:  300 GRNDFLGIAYLTVGSICLFLAVTFAVLYLVKPRQLGDPSYLSWNRSAGG 348

[Close]

BLAST of GR916794 vs. SwissProt
Match: ALIS4_ARATH (Putative ALA-interacting subunit 4 OS=Arabidopsis thaliana GN=ALIS4 PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 9.347e-17
Identity = 35/49 (71.43%), Postives = 44/49 (89.80%), Query Frame = -1

Query:  124 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGG 270
            G+NDFLGIAYLTVG +C FL+++F+++Y  KPRQLGDPSYLSWNR+ GG
Sbjct:  303 GRNDFLGIAYLTVGSICLFLAVSFSVLYLAKPRQLGDPSYLSWNRSAGG 351

[Close]

BLAST of GR916794 vs. SwissProt
Match: YNI7_SCHPO (Uncharacterized protein C11B10.07c OS=Schizosaccharomyces pombe GN=SPBC11B10.07c PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 7.173e-9
Identity = 25/45 (55.56%), Postives = 31/45 (68.89%), Query Frame = -1

Query:  136 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNR 270
            GKN FLGI Y  +GGLC    +  +I   +KPR++GDP YLSWNR
Sbjct:  323 GKNYFLGILYFVIGGLCAASGVILSIACLIKPRRVGDPRYLSWNR 367

[Close]

BLAST of GR916794 vs. SwissProt
Match: MUG89_SCHPO (Meiotically up-regulated gene 89 protein OS=Schizosaccharomyces pombe GN=mug89 PE=1 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 1.353e-7
Identity = 22/44 (50.00%), Postives = 31/44 (70.45%), Query Frame = -1

Query:  139 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWN 270
            GKN FLGIAY+ V  +C  L   FT+ +F++PR+L D  YL+W+
Sbjct:  339 GKNPFLGIAYIVVSAVCVVLGTVFTLRHFIRPRKLADHRYLNWD 382

[Close]

BLAST of GR916794 vs. TrEMBL
Match: D7T121_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_85.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00036722001 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 3.290e-20
Identity = 43/50 (86.00%), Postives = 48/50 (96.00%), Query Frame = -1

Query:  121 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGGH 270
            GKNDFLGIAYLTVGG+C FL++AFT+VY VKPR+LGDPSYLSWNRNPGGH
Sbjct:  300 GKNDFLGIAYLTVGGICIFLAMAFTVVYLVKPRRLGDPSYLSWNRNPGGH 349

[Close]

BLAST of GR916794 vs. TrEMBL
Match: D7KNX3_ARALY (LEM3 (Ligand-effect modulator 3) family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_892652 PE=4 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 5.611e-20
Identity = 44/49 (89.80%), Postives = 48/49 (97.96%), Query Frame = -1

Query:  124 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGG 270
            GKNDFLGIAYLTVGG+CFFL+LAFTI+Y VKPR+LGDPSYLSWNRNPGG
Sbjct:  300 GKNDFLGIAYLTVGGICFFLALAFTIMYLVKPRRLGDPSYLSWNRNPGG 348

[Close]

BLAST of GR916794 vs. TrEMBL
Match: C6T8B4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.785e-19
Identity = 44/50 (88.00%), Postives = 47/50 (94.00%), Query Frame = -1

Query:  121 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGGH 270
            GKNDFLGIAYLTVGGL FFLS+AFT+VY VK RQLGDPSYLSWNR+PGGH
Sbjct:  295 GKNDFLGIAYLTVGGLSFFLSMAFTVVYLVKSRQLGDPSYLSWNRSPGGH 344

[Close]

BLAST of GR916794 vs. TrEMBL
Match: B9RRQ2_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1426850 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 8.102e-19
Identity = 40/49 (81.63%), Postives = 47/49 (95.92%), Query Frame = -1

Query:  124 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGG 270
            GKNDFLGIAYLTVGG+CFFL+++FT+VY +KPR+LGDPSYLSWNR PGG
Sbjct:  301 GKNDFLGIAYLTVGGICFFLAMSFTVVYLIKPRRLGDPSYLSWNRTPGG 349

[Close]

BLAST of GR916794 vs. TrEMBL
Match: D7L0J9_ARALY (LEM3 (Ligand-effect modulator 3) family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_478636 PE=4 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.805e-18
Identity = 41/49 (83.67%), Postives = 47/49 (95.92%), Query Frame = -1

Query:  124 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGG 270
            GKNDFLGIAYLTVGG+CFFL+LAFT++Y VKPR+LGDP+YLSWNR PGG
Sbjct:  302 GKNDFLGIAYLTVGGICFFLALAFTVMYLVKPRRLGDPTYLSWNRIPGG 350

[Close]

BLAST of GR916794 vs. TrEMBL
Match: B9HYB5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_822328 PE=4 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 4.446e-17
Identity = 39/50 (78.00%), Postives = 45/50 (90.00%), Query Frame = -1

Query:  121 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGGH 270
            G+NDFLGIAYLTVG +CF LS+ FTIVYFVKPR+LGDP++LSWNR PG H
Sbjct:  301 GRNDFLGIAYLTVGMICFALSMGFTIVYFVKPRRLGDPTFLSWNRGPGSH 350

[Close]

BLAST of GR916794 vs. TrEMBL
Match: Q6H511_ORYSJ (LEM3 (Ligand-effect modulator 3)-like OS=Oryza sativa subsp. japonica GN=OSJNBa0073A21.17 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 2.207e-16
Identity = 36/49 (73.47%), Postives = 45/49 (91.84%), Query Frame = -1

Query:  124 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGG 270
            GKNDFLG+AYL+VGGLCFFL+ AFT++Y +KPR++GD +YLSWNRNP G
Sbjct:  301 GKNDFLGLAYLSVGGLCFFLAFAFTLLYLIKPRKMGDNNYLSWNRNPAG 349

[Close]

BLAST of GR916794 vs. TrEMBL
Match: A3A3M9_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_05573 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 2.207e-16
Identity = 36/49 (73.47%), Postives = 45/49 (91.84%), Query Frame = -1

Query:  124 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGG 270
            GKNDFLG+AYL+VGGLCFFL+ AFT++Y +KPR++GD +YLSWNRNP G
Sbjct:  285 GKNDFLGLAYLSVGGLCFFLAFAFTLLYLIKPRKMGDNNYLSWNRNPAG 333

[Close]

BLAST of GR916794 vs. TrEMBL
Match: A2X1G2_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_06044 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 2.207e-16
Identity = 36/49 (73.47%), Postives = 45/49 (91.84%), Query Frame = -1

Query:  124 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGG 270
            GKNDFLG+AYL+VGGLCFFL+ AFT++Y +KPR++GD +YLSWNRNP G
Sbjct:  285 GKNDFLGLAYLSVGGLCFFLAFAFTLLYLIKPRKMGDNNYLSWNRNPAG 333

[Close]

BLAST of GR916794 vs. TrEMBL
Match: B6TU52_MAIZE (Cell division control protein 50 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 2.882e-16
Identity = 37/49 (75.51%), Postives = 44/49 (89.80%), Query Frame = -1

Query:  124 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGG 270
            GKNDFLG+AYLTVGGLCFFL+ AFT++Y +KPR++GD SYLSWNR P G
Sbjct:  300 GKNDFLGLAYLTVGGLCFFLAFAFTLLYLIKPRKMGDNSYLSWNRPPSG 348

[Close]

BLAST of GR916794 vs. TAIR peptide
Match: AT1G54320.1 (| Symbols: | LEM3 (ligand-effect modulator 3) family protein / CDC50 family protein | chr1:20275732-20277793 REVERSE LENGTH=349)

HSP 1 Score: 98.2117 bits (243), Expect = 1.097e-21
Identity = 43/49 (87.76%), Postives = 47/49 (95.92%), Query Frame = -1

Query:  124 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGG 270
            GKNDFLGIAYLTVGG+CF L+LAFTI+Y VKPR+LGDPSYLSWNRNPGG
Sbjct:  300 GKNDFLGIAYLTVGGICFILALAFTIMYLVKPRRLGDPSYLSWNRNPGG 348

[Close]

BLAST of GR916794 vs. TAIR peptide
Match: AT3G12740.1 (| Symbols: ALIS1 | ALA-interacting subunit 1 | chr3:4049739-4051561 FORWARD LENGTH=350)

HSP 1 Score: 92.8189 bits (229), Expect = 4.608e-20
Identity = 40/49 (81.63%), Postives = 46/49 (93.88%), Query Frame = -1

Query:  124 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGG 270
            GKNDFLGIAYLTVGG+CF L+LAFT++Y VKPR+LGDP+YLSWNR PGG
Sbjct:  301 GKNDFLGIAYLTVGGICFVLALAFTVMYLVKPRRLGDPTYLSWNRIPGG 349

[Close]

BLAST of GR916794 vs. TAIR peptide
Match: AT1G79450.2 (| Symbols: ALIS5 | ALA-interacting subunit 5 | chr1:29888665-29889904 FORWARD LENGTH=283)

HSP 1 Score: 86.2705 bits (212), Expect = 4.313e-18
Identity = 36/49 (73.47%), Postives = 43/49 (87.76%), Query Frame = -1

Query:  124 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGG 270
            G+NDFLGIAYLTVG +C FL++ F ++Y VKPRQLGDPSYLSWNR+ GG
Sbjct:  233 GRNDFLGIAYLTVGSICLFLAVTFAVLYLVKPRQLGDPSYLSWNRSAGG 281

[Close]

BLAST of GR916794 vs. TAIR peptide
Match: AT1G79450.1 (| Symbols: ALIS5 | ALA-interacting subunit 5 | chr1:29887831-29889904 FORWARD LENGTH=350)

HSP 1 Score: 86.2705 bits (212), Expect = 4.313e-18
Identity = 36/49 (73.47%), Postives = 43/49 (87.76%), Query Frame = -1

Query:  124 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGG 270
            G+NDFLGIAYLTVG +C FL++ F ++Y VKPRQLGDPSYLSWNR+ GG
Sbjct:  300 GRNDFLGIAYLTVGSICLFLAVTFAVLYLVKPRQLGDPSYLSWNRSAGG 348

[Close]

BLAST of GR916794 vs. TAIR peptide
Match: AT1G16360.1 (| Symbols: | LEM3 (ligand-effect modulator 3) family protein / CDC50 family protein | chr1:5593493-5595179 REVERSE LENGTH=336)

HSP 1 Score: 85.5001 bits (210), Expect = 7.357e-18
Identity = 35/49 (71.43%), Postives = 44/49 (89.80%), Query Frame = -1

Query:  124 GKNDFLGIAYLTVGGLCFFLSLAFTIVYFVKPRQLGDPSYLSWNRNPGG 270
            G+NDFLGIAYLTVG +C FL+++F+++Y  KPRQLGDPSYLSWNR+ GG
Sbjct:  286 GRNDFLGIAYLTVGSICLFLAVSFSVLYLAKPRQLGDPSYLSWNRSAGG 334

The following BLAST results are available for this feature:

BLAST of GR916794 vs. SwissProt
BLAST of GR916794 vs. TrEMBL
BLAST of GR916794 vs. TAIR peptide

BLAST of GR916794 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 6


Match Name	E-value	Identity	Description
ALIS3_ARATH	1.393e-20	87.76	ALA-interacting subunit 3 OS=Arabidopsis thaliana ...	[more]
ALIS1_ARATH	5.855e-19	81.63	ALA-interacting subunit 1 OS=Arabidopsis thaliana ...	[more]
ALIS5_ARATH	5.480e-17	73.47	ALA-interacting subunit 5 OS=Arabidopsis thaliana ...	[more]
ALIS4_ARATH	9.347e-17	71.43	Putative ALA-interacting subunit 4 OS=Arabidopsis ...	[more]
YNI7_SCHPO	7.173e-9	55.56	Uncharacterized protein C11B10.07c OS=Schizosaccha...	[more]
MUG89_SCHPO	1.353e-7	50.00	Meiotically up-regulated gene 89 protein OS=Schizo...	[more]

BLAST of GR916794 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7T121_VITVI	3.290e-20	86.00	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7KNX3_ARALY	5.611e-20	89.80	LEM3 (Ligand-effect modulator 3) family protein OS...	[more]
C6T8B4_SOYBN	2.785e-19	88.00	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9RRQ2_RICCO	8.102e-19	81.63	Putative uncharacterized protein OS=Ricinus commun...	[more]
D7L0J9_ARALY	1.805e-18	83.67	LEM3 (Ligand-effect modulator 3) family protein OS...	[more]
B9HYB5_POPTR	4.446e-17	78.00	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q6H511_ORYSJ	2.207e-16	73.47	LEM3 (Ligand-effect modulator 3)-like OS=Oryza sat...	[more]
A3A3M9_ORYSJ	2.207e-16	73.47	Putative uncharacterized protein OS=Oryza sativa s...	[more]
A2X1G2_ORYSI	2.207e-16	73.47	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B6TU52_MAIZE	2.882e-16	75.51	Cell division control protein 50 OS=Zea mays PE=2 ...	[more]

BLAST of GR916794 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT1G54320.1	1.097e-21	87.76	\| Symbols: \| LEM3 (ligand-effect modulator 3) fam...	[more]
AT3G12740.1	4.608e-20	81.63	\| Symbols: ALIS1 \| ALA-interacting subunit 1 \| chr...	[more]
AT1G79450.2	4.313e-18	73.47	\| Symbols: ALIS5 \| ALA-interacting subunit 5 \| chr...	[more]
AT1G79450.1	4.313e-18	73.47	\| Symbols: ALIS5 \| ALA-interacting subunit 5 \| chr...	[more]
AT1G16360.1	7.357e-18	71.43	\| Symbols: \| LEM3 (ligand-effect modulator 3) fam...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005045	Protein of unknown function DUF284, transmembrane eukaryotic	PANTHER	PTHR10926	CDC50-RELATED	coord: 9..58 score: 7.2
IPR005045	Protein of unknown function DUF284, transmembrane eukaryotic	PFAM	PF03381	CDC50	coord: 9..46 score: 3.8

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR916794 ID=GR916794; Name=GR916794; organism=Cicer arietinum; type=EST; length=294bp
ATTAGCGTGGTCGCGGCCGAGGNACAAAAACTACTTCCCATCTGTCACTG
GAGAATAACCAAGCCCTGTAGTGGGGGAAATTGGAATTAACGAACATAGC
ACAATTGGTATTTTTGCTTAATGTCCTCCTGGGTTCCTATTCCATGACAA
ATATGAAGGATCTCCTAGTTGCCTTGGCTTGACGAAATATACAATGGTAA
AAGCCAAAGACAAAAAGAAGCATAATCCTCCGACAGTGAGATAAGCAATG
CCAAGGAAGTCATTCTTCCCCCGCGTACCTGCCCGGGCGGCCGC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR005045	CDC50/LEM3_fam

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