FE672170

EST Overview


Name	FE672170
Unique Name	FE672170
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	574

Libraries


Library Name	Type
LIBEST_022820 Drought-stressed chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of FE672170 vs. SwissProt
Match: VPS33_ARATH (Vacuolar protein sorting-associated protein 33 homolog OS=Arabidopsis thaliana GN=VPS33 PE=2 SV=1)

HSP 1 Score: 291.967 bits (746), Expect = 1.436e-78
Identity = 139/189 (73.54%), Postives = 162/189 (85.71%), Query Frame = 1

Query:    7 FLGQLDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQESKSNWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRSGWRPVEEILKLLPGPHLEIRRGGFLNSPSSDTLSGVSTSIAKVPDGRRALVLV 573
            F  QLDME T++EA++YDIC+EYIEE+IHKQEPL  VLRLL+LFS+TN+GLPKK FDY R ELLHSYGFEH+ TLNNLEKAGL KKQE KSNWLT+KR L+L+VEDTDT+ PNDIAYV+SGYAPLSIRLIQ A  SGWRP+E+ILKLLPGPHLE +R GF +SPS D+L G S  +A   DGRR++VLV
Sbjct:  358 FNSQLDMEQTLVEAENYDICYEYIEEMIHKQEPLTNVLRLLVLFSVTNSGLPKKQFDYIRMELLHSYGFEHVVTLNNLEKAGLLKKQEFKSNWLTVKRTLKLIVEDTDTSRPNDIAYVYSGYAPLSIRLIQQAIHSGWRPMEDILKLLPGPHLETKRSGFPSSPSVDSLHGASNGVA---DGRRSIVLV 543

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BLAST of FE672170 vs. SwissProt
Match: VP33A_MOUSE (Vacuolar protein sorting-associated protein 33A OS=Mus musculus GN=Vps33a PE=2 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 5.897e-40
Identity = 80/158 (50.63%), Postives = 112/158 (70.89%), Query Frame = 1

Query:    7 FLGQLDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQE-SKSNWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRSGWRPVEEILKLLPGPHLEIRR 477
            F  +L +E   +     D    YIE+ I ++ PLI VLRL+ L S+ N+GL +K  DY+RRE+L +YG+EHI TLNNLEKAGL K Q   ++N+ TI++ L+L ++D +  NP DI+YV+SGYAPLS+RL Q  +R GWR +EE+L++LPGPH E R+
Sbjct:  372 FFDKLTVEQEFMSGIDTDKVNSYIEDCIAQKHPLIKVLRLVCLQSVCNSGLKQKVLDYYRREILQTYGYEHILTLNNLEKAGLLKAQTGGRNNYPTIRKTLRLWMDDVNEQNPTDISYVYSGYAPLSVRLAQLLSRPGWRSIEEVLRILPGPHFEERQ 529

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BLAST of FE672170 vs. SwissProt
Match: VP33A_RAT (Vacuolar protein sorting-associated protein 33A OS=Rattus norvegicus GN=Vps33a PE=1 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 2.241e-39
Identity = 79/158 (50.00%), Postives = 112/158 (70.89%), Query Frame = 1

Query:    7 FLGQLDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQE-SKSNWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRSGWRPVEEILKLLPGPHLEIRR 477
            F  +L +E   +     D    YIE+ I ++ PLI VLRL+ L S+ N+GL +K  DY++RE+L +YG+EHI TLNNLEKAGL K Q   ++N+ TI++ L+L ++D +  NP DI+YV+SGYAPLS+RL Q  +R GWR +EE+L++LPGPH E R+
Sbjct:  371 FFDKLTVEQEFMSGIDTDKVNNYIEDCIAQKHPLIKVLRLVCLQSMCNSGLKQKVLDYYKREILQTYGYEHILTLNNLEKAGLLKAQTGGRNNYPTIRKTLRLWMDDVNEQNPTDISYVYSGYAPLSVRLAQLLSRPGWRSIEEVLRILPGPHFEERQ 528

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BLAST of FE672170 vs. SwissProt
Match: VP33A_HUMAN (Vacuolar protein sorting-associated protein 33A OS=Homo sapiens GN=VPS33A PE=1 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 4.226e-38
Identity = 77/158 (48.73%), Postives = 111/158 (70.25%), Query Frame = 1

Query:    7 FLGQLDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQES-KSNWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRSGWRPVEEILKLLPGPHLEIRR 477
            F  +L +E   +     D    YIE+ I ++  LI VLRL+ L S+ N+GL +K  DY++RE+L +YG+EHI TL+NLEKAGL K Q   ++N+ TI++ L+L ++D +  NP DI+YV+SGYAPLS+RL Q  +R GWR +EE+L++LPGPH E R+
Sbjct:  370 FFDKLTVEQEFMSGIDTDKVNNYIEDCIAQKHSLIKVLRLVCLQSVCNSGLKQKVLDYYKREILQTYGYEHILTLHNLEKAGLLKPQTGGRNNYPTIRKTLRLWMDDVNEQNPTDISYVYSGYAPLSVRLAQLLSRPGWRSIEEVLRILPGPHFEERQ 527

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BLAST of FE672170 vs. SwissProt
Match: VP33A_DROME (Vacuolar protein sorting-associated protein 33A OS=Drosophila melanogaster GN=car PE=1 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 2.752e-21
Identity = 60/184 (32.61%), Postives = 98/184 (53.26%), Query Frame = 1

Query:    1 IPFLGQLDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQESKSNWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRS-GWRPVEEILKLLPGPHLEIRRGGFLNSPSSDTLSGVST-SIAKVP 546
            + F   L  E   +     D    YIE+LI  +  L  VLRL+ +     +G  +K  ++++REL+H YG E + T++NLEK+GL   Q     +  +++ L L V+D     P DI+YV S YAPL+ R+++H+ +  GW+ ++  +  LPGP  E  +   +      T++ VS  S+  VP
Sbjct:  382 VRFADDLAAEQEFMVCADIDKPSAYIEDLIACRVELNRVLRLICMQCHAASGFKEKLLNHYKRELVHVYGLEVLLTISNLEKSGLLHLQTESRAYSVLRKTLHLTVDDNVEIEPKDISYVHSFYAPLTARIVEHSLKPLGWQTLKSQINNLPGPTFEDFQAQLVGIGGRHTVTTVSEGSLLNVP 565

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BLAST of FE672170 vs. SwissProt
Match: VP33B_DANRE (Vacuolar protein sorting-associated protein 33B OS=Danio rerio GN=vps33b PE=2 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 8.853e-20
Identity = 67/204 (32.84%), Postives = 99/204 (48.53%), Query Frame = 1

Query:   19 LDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQES--------------------------------KSNWLTIKRALQLVV---EDTDTANPNDIAYVFSG-YAPLSIRLIQHA-TRSGWRPVEEILKLLPGPHLEIRRGGFLNSPSSDTLSG 519
            L  EH+++E      C  YIEE I++Q  +I  LRLL L SIT  GL  K +   + + L SYG EH+ T  NL + GL ++Q++                                KSN+  + + L LV    E+ D   P D+AY+FSG Y PLS +LI+    R GW  +EE+ ++L G    +  GG  +S + +  +G
Sbjct:  362 LKTEHSLLEGFEIRECIAYIEEHINRQVSMIDSLRLLCLLSITENGLLSKDYRSLKAQYLQSYGIEHLLTFANLRQLGLLEEQQTGETLTVMESKVGKLVNDKTAGKLTDAFSSLAKKSNFRALSKRLALVPKSGEEYDLRVPRDMAYIFSGAYIPLSCKLIEQVLERDGWTGLEEVTRMLNGQDFAV-TGGSSSSEARNKSNG 564

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BLAST of FE672170 vs. SwissProt
Match: VPS33_SCHPO (Vacuolar protein sorting-associated protein 33 OS=Schizosaccharomyces pombe GN=vps33 PE=2 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 9.788e-19
Identity = 54/159 (33.96%), Postives = 88/159 (55.35%), Query Frame = 1

Query:   19 LDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQESKS-------NWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRSGWRPVEEILKL--LPGPHLE 468
            L ++H ++        F  ++E+I+ + P+  V R+L L SIT  GL +K  D++RRE+  +YG+ H+ T   L  AGL + ++S +       ++ T      LV ++ D  NP DIAY +SGY PLS+  I +    G    E+ILKL  +PG +++
Sbjct:  357 LQLQHLLVSHADSFTQFNLLDEIIYAEAPVEEVFRVLCLASITTNGLRRKDIDHYRREITQTYGYYHLLTFQALIDAGLLRLRQSTNISLQKSLSYSTWLNTYPLVKDEVDEQNPEDIAYTYSGYGPLSVH-IAYDILKGRDNEEKILKLQNMPGTYVD 514

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BLAST of FE672170 vs. SwissProt
Match: VP33B_RAT (Vacuolar protein sorting-associated protein 33B OS=Rattus norvegicus GN=Vps33b PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.013e-15
Identity = 59/178 (33.15%), Postives = 85/178 (47.75%), Query Frame = 1

Query:   28 EHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQES--------------------------------KSNWLTIKRALQLVVE---DTDTANPNDIAYVFSG-YAPLSIRLIQHA-TRSGWRPVEEILKLL 450
            EH ++E  +      YIEE I +Q   I  LRL+ L SIT  GL  K +   + + L SYG EH+ T +NL +AGL  +Q S                                +SN+  I + L L+     + D   P D+AYVFSG Y PLS R+I+    R  W+ ++E+++LL
Sbjct:  367 EHALLEGFNIRESTSYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKTQYLQSYGPEHLLTFSNLRRAGLLTEQASGDTLTAVENKVSKLVTDKAAGKITDAFSSLAKRSNFRAISKKLNLIPRVDGEYDLKVPRDMAYVFSGAYVPLSCRIIEQVLDRRSWQGLDEVVRLL 544

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BLAST of FE672170 vs. SwissProt
Match: VP33B_BOVIN (Vacuolar protein sorting-associated protein 33B OS=Bos taurus GN=VPS33B PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.013e-15
Identity = 62/178 (34.83%), Postives = 86/178 (48.31%), Query Frame = 1

Query:   28 EHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQ---------ESK-----------------------SNWLTIKRALQLVVE---DTDTANPNDIAYVFSG-YAPLSIRLIQHA-TRSGWRPVEEILKLL 450
            EH ++E  +      YIEE I +Q   I  LRL+ L SIT  GL  K +   + + L SYG EH+ T  NL +AGL  +Q         ESK                       SN+  I + L L+     + D   P D+AYVFSG Y PLS R+I+    R GW+ ++E+++LL
Sbjct:  367 EHALLEGFNIRESTNYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKTQYLQSYGPEHLLTFFNLRRAGLLTEQAPGDTLTAVESKVSKLVTDKAAGKITDAFSSLAKRSNFRAISKKLNLIPRVDGEYDLKVPRDMAYVFSGAYVPLSCRIIEQVLERRGWQGLDEVVRLL 544

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BLAST of FE672170 vs. SwissProt
Match: VP33B_MOUSE (Vacuolar protein sorting-associated protein 33B OS=Mus musculus GN=Vps33b PE=1 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.728e-15
Identity = 61/178 (34.27%), Postives = 86/178 (48.31%), Query Frame = 1

Query:   28 EHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQ---------ESK-----------------------SNWLTIKRALQLVVE---DTDTANPNDIAYVFSG-YAPLSIRLIQHA-TRSGWRPVEEILKLL 450
            EH ++E  +      YIEE I +Q   I  LRL+ L SIT  GL  K +   + + L SYG EH+ T +NL +AGL  +Q         ESK                       SN+  I + L L+     + D   P D+AYVFSG Y PLS R+I+    R  W+ ++E+++LL
Sbjct:  367 EHALLEGFNIRESTSYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKTQYLQSYGPEHLLTFSNLRRAGLLTEQAPGDTLTAVESKVSKLVTDKAAGKITDAFSSLAKRSNFRAISKKLNLIPRVDGEYDLKVPRDMAYVFSGAYVPLSCRIIEQVLDRRSWQGLDEVVRLL 544

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BLAST of FE672170 vs. TrEMBL
Match: D7TM58_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_19.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00016224001 PE=4 SV=1)

HSP 1 Score: 322.013 bits (824), Expect = 1.795e-86
Identity = 156/189 (82.54%), Postives = 171/189 (90.48%), Query Frame = 1

Query:    7 FLGQLDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQESKSNWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRSGWRPVEEILKLLPGPHLEIRRGGFLNSPSSDTLSGVSTSIAKVPDGRRALVLV 573
            FLG+LDMEHTI+EAQSYDICFEYIEE+IHKQEPL+ VLRLLILFSITN+GLPK++FDY RRELLHSYGFEH+ATLNNLEKAGL KKQE+KSNWLTIKRALQLVVEDTDT NPNDIAYVFSGYAPLSIRL+Q A RSGWRP+EEILKLLPGPH E +R GF +S S D L+G   SI KV DGRR+LVLV
Sbjct:  360 FLGRLDMEHTIVEAQSYDICFEYIEEMIHKQEPLVNVLRLLILFSITNSGLPKRNFDYLRRELLHSYGFEHMATLNNLEKAGLLKKQETKSNWLTIKRALQLVVEDTDTTNPNDIAYVFSGYAPLSIRLVQQAVRSGWRPIEEILKLLPGPHSETKRSGFASSQSIDNLAGAPASIDKVTDGRRSLVLV 548

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BLAST of FE672170 vs. TrEMBL
Match: B9S0W7_RICCO (Vacuolar protein sorting, putative OS=Ricinus communis GN=RCOM_1538620 PE=4 SV=1)

HSP 1 Score: 318.546 bits (815), Expect = 1.984e-85
Identity = 150/189 (79.37%), Postives = 174/189 (92.06%), Query Frame = 1

Query:    7 FLGQLDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQESKSNWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRSGWRPVEEILKLLPGPHLEIRRGGFLNSPSSDTLSGVSTSIAKVPDGRRALVLV 573
            FL +LDMEHTI+EA++YD CFE+IEELIHKQEPL+TVLR+LIL S+TN+GLP+K+FDY RRELLHSYGFEH+ATLNNLEKAGL KKQES+SNW+TIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRL+QHA RSGWRP+EEILKLLPGPH E +R GF +SPSS+TL+G   ++ +V DGRR+LVLV
Sbjct:  360 FLARLDMEHTIVEAENYDACFEHIEELIHKQEPLVTVLRMLILLSVTNSGLPRKNFDYIRRELLHSYGFEHMATLNNLEKAGLLKKQESRSNWVTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLVQHAVRSGWRPMEEILKLLPGPHSETKRAGFTSSPSSNTLNGAGATVDRVADGRRSLVLV 548

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BLAST of FE672170 vs. TrEMBL
Match: B9HUI9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1092514 PE=4 SV=1)

HSP 1 Score: 315.464 bits (807), Expect = 1.680e-84
Identity = 153/189 (80.95%), Postives = 171/189 (90.48%), Query Frame = 1

Query:    7 FLGQLDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQESKSNWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRSGWRPVEEILKLLPGPHLEIRRGGFLNSPSSDTLSGVSTSIAKVPDGRRALVLV 573
            FL +LDME T+IEAQSYDICF+YIEE IHKQEPL++VLRLLILFSITN+GLPK++FD+ RRELLHSYGFEHIA LNNLEKAGL KKQE+KSNWLTIKR LQLVVEDTDTANPNDIAYVFSGYAPLSIRL+Q A RSGWRP+EEILKLLPGPH E +RGGF +SPS DTL G S ++ +V DGRR+LVLV
Sbjct:  359 FLSRLDMEQTLIEAQSYDICFDYIEESIHKQEPLVSVLRLLILFSITNSGLPKRNFDHLRRELLHSYGFEHIAMLNNLEKAGLLKKQENKSNWLTIKRTLQLVVEDTDTANPNDIAYVFSGYAPLSIRLVQQAVRSGWRPMEEILKLLPGPHSETKRGGFSSSPSFDTLHGASAAVDRVADGRRSLVLV 547

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BLAST of FE672170 vs. TrEMBL
Match: B9HLH4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_563704 PE=4 SV=1)

HSP 1 Score: 302.368 bits (773), Expect = 1.472e-80
Identity = 149/198 (75.25%), Postives = 168/198 (84.85%), Query Frame = 1

Query:    7 FLGQLDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQESKSNWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRSGWRPVEEILKLLPGPHLEIRRGGFLNSPSSDTLSGVSTSIAK---------VPDGRRALVLV 573
            F  +LDME T++EAQSYDIC++YIEELIHKQEPL+TV+RLLILFSITN+GLPKK+FD+ RRELLHSYGFEHIA LNNLEKAGL KKQESK NWLTIKR LQLV EDTDT NPNDIAYVFSGYAPLSIRL+QHA RSGWRP+EE LKLLPGPH E++RGGF +S S DTL G S ++ +         V DGRR+LVLV
Sbjct:  359 FQSRLDMEQTLVEAQSYDICYDYIEELIHKQEPLVTVVRLLILFSITNSGLPKKNFDHLRRELLHSYGFEHIAMLNNLEKAGLLKKQESKGNWLTIKRNLQLVTEDTDTTNPNDIAYVFSGYAPLSIRLVQHAVRSGWRPMEETLKLLPGPHSEMKRGGFSSSSSFDTLHGASAAVDRHVQTHPGFLVADGRRSLVLV 556

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BLAST of FE672170 vs. TrEMBL
Match: D7LUY5_ARALY (ATVPS33 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_485899 PE=4 SV=1)

HSP 1 Score: 295.049 bits (754), Expect = 2.350e-78
Identity = 142/189 (75.13%), Postives = 162/189 (85.71%), Query Frame = 1

Query:    7 FLGQLDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQESKSNWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRSGWRPVEEILKLLPGPHLEIRRGGFLNSPSSDTLSGVSTSIAKVPDGRRALVLV 573
            FL QLDME T++EA+SYDIC+EYIEE+IHKQE L  VLRLL+LFS+TN+GLPKK FDY R ELLHSYGFEH+ TLNNLEKAGL KKQE KSNWLT+KR LQL+VEDTDT+ PNDIAYV+SGYAPLSIRLIQ A  SGWRPVE+ILKLLPGPHLE +R GF +SPS D+L G S  +A   DGRR++VLV
Sbjct:  358 FLSQLDMEQTLVEAESYDICYEYIEEMIHKQEHLTNVLRLLVLFSVTNSGLPKKQFDYIRMELLHSYGFEHVVTLNNLEKAGLLKKQEFKSNWLTVKRTLQLIVEDTDTSRPNDIAYVYSGYAPLSIRLIQQAIHSGWRPVEDILKLLPGPHLETKRSGFPSSPSVDSLHGASNGVA---DGRRSIVLV 543

[Close]

BLAST of FE672170 vs. TrEMBL
Match: Q0JEQ3_ORYSJ (Os04g0223000 protein OS=Oryza sativa subsp. japonica GN=Os04g0223000 PE=2 SV=1)

HSP 1 Score: 258.84 bits (660), Expect = 1.866e-67
Identity = 123/189 (65.08%), Postives = 154/189 (81.48%), Query Frame = 1

Query:    7 FLGQLDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQESKSNWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRSGWRPVEEILKLLPGPHLEIRRGGFLNSPSSDTLSGVSTSIAKVPDGRRALVLV 573
            F  +LD+E TI+E Q+++ICFEYIEE+IHKQEP+  VLRLL+L S+TNAGLPKK+FDY RRE+LHSYGFEH+  L NLEKAGLFK+QES+SNW+ I RALQL+V+  DTANP+DI+Y+FSGYAPLSIRL+QHA RSGWR +EE+LKLLPGPHL+++RG    + S D   G+    +    G R+LVLV
Sbjct:  360 FHARLDIEQTILEVQNFEICFEYIEEMIHKQEPIENVLRLLVLLSLTNAGLPKKNFDYLRREILHSYGFEHMPLLYNLEKAGLFKRQESRSNWIGITRALQLIVDVNDTANPSDISYIFSGYAPLSIRLVQHAVRSGWRSIEELLKLLPGPHLDLKRGSSAINSSLDVHQGLGLQQSIDRVGHRSLVLV 548

[Close]

BLAST of FE672170 vs. TrEMBL
Match: C5XNN0_SORBI (Putative uncharacterized protein Sb03g025460 OS=Sorghum bicolor GN=Sb03g025460 PE=4 SV=1)

HSP 1 Score: 254.603 bits (649), Expect = 3.519e-66
Identity = 125/191 (65.45%), Postives = 154/191 (80.63%), Query Frame = 1

Query:    7 FLGQLDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQESKSNWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRSGWRPVEEILKLLPGPHLEIRRGGFL--NSPSSDTLSGVSTSIAKVPDGRRALVLV 573
            F  +LD+E TI+E Q+++ICFEYIEE+IHKQE +  VLRLL+L S+TNAGLPKK+FDY RRE+LHSYGFEH+  L NLEKAGL K+QES+SNW+ I RALQL+V+  DTANP DI+Y+FSGYAPLSIRL+QHA RSGWR +EE+LKLLPGPHL+++RGG    +SP      G   SI +V  G R+LVLV
Sbjct:  360 FHARLDIEQTILEVQNFEICFEYIEEMIHKQEAIENVLRLLVLLSLTNAGLPKKNFDYLRREILHSYGFEHMHLLYNLEKAGLLKRQESRSNWVGITRALQLIVDVNDTANPTDISYIFSGYAPLSIRLVQHAVRSGWRSIEELLKLLPGPHLDLKRGGLTINSSPEVHPGLGAQQSIDRV--GHRSLVLV 548

[Close]

BLAST of FE672170 vs. TrEMBL
Match: B6T8U3_MAIZE (Vacuolar protein-sorting protein 33 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 254.218 bits (648), Expect = 4.596e-66
Identity = 125/191 (65.45%), Postives = 154/191 (80.63%), Query Frame = 1

Query:    7 FLGQLDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQESKSNWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRSGWRPVEEILKLLPGPHLEIRRGGFL--NSPSSDTLSGVSTSIAKVPDGRRALVLV 573
            F  +LD+E TI+E Q+++ICFEYIEE+IHKQE +  VLRLL+L S+TNAGLPKK+FDY RRE+LHSYGFEH+  L NLEKAGL K+QES+SNW+ I RALQL+V+  DTANP DI+Y+FSGYAPLSIRL+QHA RSGWR +EE+LKLLPGPHL+++RGG    +SP      G   SI +V  G R+LVLV
Sbjct:  360 FHARLDIEQTILEVQNFEICFEYIEEMIHKQEVIENVLRLLVLLSLTNAGLPKKNFDYMRREILHSYGFEHMHLLYNLEKAGLLKRQESRSNWVGITRALQLIVDVNDTANPTDISYIFSGYAPLSIRLVQHAVRSGWRSIEELLKLLPGPHLDLKRGGLTINSSPEVHPGLGAQQSIDRV--GHRSLVLV 548

[Close]

BLAST of FE672170 vs. TrEMBL
Match: Q7XQY9_ORYSJ (OSJNBb0045P24.17 protein OS=Oryza sativa subsp. japonica GN=OSJNBb0045P24.17 PE=4 SV=1)

HSP 1 Score: 253.447 bits (646), Expect = 7.840e-66
Identity = 117/172 (68.02%), Postives = 146/172 (84.88%), Query Frame = 1

Query:    7 FLGQLDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQESKSNWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRSGWRPVEEILKLLPGPHLEIRRGGFLNSPSSDTLSGV 522
            F  +LD+E TI+E Q+++ICFEYIEE+IHKQEP+  VLRLL+L S+TNAGLPKK+FDY RRE+LHSYGFEH+  L NLEKAGLFK+QES+SNW+ I RALQL+V+  DTANP+DI+Y+FSGYAPLSIRL+QHA RSGWR +EE+LKLLPGPHL+++RG    + S D   G+
Sbjct:  281 FHARLDIEQTILEVQNFEICFEYIEEMIHKQEPIENVLRLLVLLSLTNAGLPKKNFDYLRREILHSYGFEHMPLLYNLEKAGLFKRQESRSNWIGITRALQLIVDVNDTANPSDISYIFSGYAPLSIRLVQHAVRSGWRSIEELLKLLPGPHLDLKRGSSAINSSLDVHQGL 452

[Close]

BLAST of FE672170 vs. TrEMBL
Match: B9FDW1_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_13888 PE=4 SV=1)

HSP 1 Score: 253.447 bits (646), Expect = 7.840e-66
Identity = 117/172 (68.02%), Postives = 146/172 (84.88%), Query Frame = 1

Query:    7 FLGQLDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQESKSNWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRSGWRPVEEILKLLPGPHLEIRRGGFLNSPSSDTLSGV 522
            F  +LD+E TI+E Q+++ICFEYIEE+IHKQEP+  VLRLL+L S+TNAGLPKK+FDY RRE+LHSYGFEH+  L NLEKAGLFK+QES+SNW+ I RALQL+V+  DTANP+DI+Y+FSGYAPLSIRL+QHA RSGWR +EE+LKLLPGPHL+++RG    + S D   G+
Sbjct:  367 FHARLDIEQTILEVQNFEICFEYIEEMIHKQEPIENVLRLLVLLSLTNAGLPKKNFDYLRREILHSYGFEHMPLLYNLEKAGLFKRQESRSNWIGITRALQLIVDVNDTANPSDISYIFSGYAPLSIRLVQHAVRSGWRSIEELLKLLPGPHLDLKRGSSAINSSLDVHQGL 538

[Close]

BLAST of FE672170 vs. TAIR peptide
Match: AT3G54860.2 (| Symbols: ATVPS33, VPS33 | Sec1/munc18-like (SM) proteins superfamily | chr3:20324286-20329841 REVERSE LENGTH=608)

HSP 1 Score: 291.967 bits (746), Expect = 1.525e-79
Identity = 139/189 (73.54%), Postives = 162/189 (85.71%), Query Frame = 1

Query:    7 FLGQLDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQESKSNWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRSGWRPVEEILKLLPGPHLEIRRGGFLNSPSSDTLSGVSTSIAKVPDGRRALVLV 573
            F  QLDME T++EA++YDIC+EYIEE+IHKQEPL  VLRLL+LFS+TN+GLPKK FDY R ELLHSYGFEH+ TLNNLEKAGL KKQE KSNWLT+KR L+L+VEDTDT+ PNDIAYV+SGYAPLSIRLIQ A  SGWRP+E+ILKLLPGPHLE +R GF +SPS D+L G S  +A   DGRR++VLV
Sbjct:  374 FNSQLDMEQTLVEAENYDICYEYIEEMIHKQEPLTNVLRLLVLFSVTNSGLPKKQFDYIRMELLHSYGFEHVVTLNNLEKAGLLKKQEFKSNWLTVKRTLKLIVEDTDTSRPNDIAYVYSGYAPLSIRLIQQAIHSGWRPMEDILKLLPGPHLETKRSGFPSSPSVDSLHGASNGVA---DGRRSIVLV 559

[Close]

BLAST of FE672170 vs. TAIR peptide
Match: AT3G54860.1 (| Symbols: ATVPS33, VPS33 | Sec1/munc18-like (SM) proteins superfamily | chr3:20324286-20329841 REVERSE LENGTH=592)

HSP 1 Score: 291.967 bits (746), Expect = 1.525e-79
Identity = 139/189 (73.54%), Postives = 162/189 (85.71%), Query Frame = 1

Query:    7 FLGQLDMEHTIIEAQSYDICFEYIEELIHKQEPLITVLRLLILFSITNAGLPKKHFDYFRRELLHSYGFEHIATLNNLEKAGLFKKQESKSNWLTIKRALQLVVEDTDTANPNDIAYVFSGYAPLSIRLIQHATRSGWRPVEEILKLLPGPHLEIRRGGFLNSPSSDTLSGVSTSIAKVPDGRRALVLV 573
            F  QLDME T++EA++YDIC+EYIEE+IHKQEPL  VLRLL+LFS+TN+GLPKK FDY R ELLHSYGFEH+ TLNNLEKAGL KKQE KSNWLT+KR L+L+VEDTDT+ PNDIAYV+SGYAPLSIRLIQ A  SGWRP+E+ILKLLPGPHLE +R GF +SPS D+L G S  +A   DGRR++VLV
Sbjct:  358 FNSQLDMEQTLVEAENYDICYEYIEEMIHKQEPLTNVLRLLVLFSVTNSGLPKKQFDYIRMELLHSYGFEHVVTLNNLEKAGLLKKQEFKSNWLTVKRTLKLIVEDTDTSRPNDIAYVYSGYAPLSIRLIQQAIHSGWRPMEDILKLLPGPHLETKRSGFPSSPSVDSLHGASNGVA---DGRRSIVLV 543

The following BLAST results are available for this feature:

BLAST of FE672170 vs. SwissProt
BLAST of FE672170 vs. TrEMBL
BLAST of FE672170 vs. TAIR peptide

BLAST of FE672170 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
VPS33_ARATH	1.436e-78	73.54	Vacuolar protein sorting-associated protein 33 hom...	[more]
VP33A_MOUSE	5.897e-40	50.63	Vacuolar protein sorting-associated protein 33A OS...	[more]
VP33A_RAT	2.241e-39	50.00	Vacuolar protein sorting-associated protein 33A OS...	[more]
VP33A_HUMAN	4.226e-38	48.73	Vacuolar protein sorting-associated protein 33A OS...	[more]
VP33A_DROME	2.752e-21	32.61	Vacuolar protein sorting-associated protein 33A OS...	[more]
VP33B_DANRE	8.853e-20	32.84	Vacuolar protein sorting-associated protein 33B OS...	[more]
VPS33_SCHPO	9.788e-19	33.96	Vacuolar protein sorting-associated protein 33 OS=...	[more]
VP33B_RAT	1.013e-15	33.15	Vacuolar protein sorting-associated protein 33B OS...	[more]
VP33B_BOVIN	1.013e-15	34.83	Vacuolar protein sorting-associated protein 33B OS...	[more]
VP33B_MOUSE	1.728e-15	34.27	Vacuolar protein sorting-associated protein 33B OS...	[more]

BLAST of FE672170 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7TM58_VITVI	1.795e-86	82.54	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9S0W7_RICCO	1.984e-85	79.37	Vacuolar protein sorting, putative OS=Ricinus comm...	[more]
B9HUI9_POPTR	1.680e-84	80.95	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9HLH4_POPTR	1.472e-80	75.25	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7LUY5_ARALY	2.350e-78	75.13	ATVPS33 OS=Arabidopsis lyrata subsp. lyrata GN=ARA...	[more]
Q0JEQ3_ORYSJ	1.866e-67	65.08	Os04g0223000 protein OS=Oryza sativa subsp. japoni...	[more]
C5XNN0_SORBI	3.519e-66	65.45	Putative uncharacterized protein Sb03g025460 OS=So...	[more]
B6T8U3_MAIZE	4.596e-66	65.45	Vacuolar protein-sorting protein 33 OS=Zea mays PE...	[more]
Q7XQY9_ORYSJ	7.840e-66	68.02	OSJNBb0045P24.17 protein OS=Oryza sativa subsp. ja...	[more]
B9FDW1_ORYSJ	7.840e-66	68.02	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of FE672170 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT3G54860.2	1.525e-79	73.54	\| Symbols: ATVPS33, VPS33 \| Sec1/munc18-like (SM) ...	[more]
AT3G54860.1	1.525e-79	73.54	\| Symbols: ATVPS33, VPS33 \| Sec1/munc18-like (SM) ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001619	Sec1-like protein	PANTHER	PTHR11679	VESICLE PROTEIN SORTING-ASSOCIATED	coord: 3..191 score: 1.3
IPR001619	Sec1-like protein	PFAM	PF00995	Sec1	coord: 8..147 score: 2.5
IPR001619	Sec1-like protein	SUPERFAMILY	56815	Sec1/munc18-like (SM) proteins	coord: 3..156 score: 2.5
None	No IPR available	PANTHER	PTHR11679:SF1	VACUOLAR PROTEIN SORTING (VPS33)	coord: 3..191 score: 1.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE672170 ID=FE672170; Name=FE672170; organism=Cicer arietinum; type=EST; length=574bp
ATTCCTTTTCTTGGGCAGCTTGATATGGAACATACAATAATTGAGGCCCA
GAGTTATGACATATGCTTTGAGTATATTGAAGAATTGATCCATAAGCAGG
AGCCTTTAATAACAGTCCTACGTCTTCTAATCCTATTTTCTATCACTAAT
GCTGGGTTGCCGAAGAAGCATTTTGACTACTTCAGGAGAGAGCTACTGCA
CAGCTATGGATTTGAGCACATAGCAACGCTAAATAATTTAGAGAAAGCGG
GACTCTTTAAGAAGCAGGAGTCAAAAAGCAATTGGCTTACCATAAAACGT
GCCTTGCAGCTTGTGGTTGAAGACACTGATACAGCCAACCCCAATGACAT
TGCTTACGTCTTCTCTGGATATGCACCACTTAGCATTCGTCTTATCCAGC
ATGCCACTCGATCTGGATGGCGCCCTGTTGAAGAAATTTTGAAACTACTG
CCTGGACCTCATTTGGAAATTAGAAGGGGTGGATTCTTAAACAGCCCATC
ATCTGACACTTTATCTGGGGTTTCAACCAGTATAGCCAAAGTACCTGATG
GAAGGCGTGCCCTGGTGCTTGTTG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006904	vesicle docking involved in exocytosis
GO:0016192	vesicle-mediated transport

Vocabulary: **INTERPRO**
Term	Definition
IPR001619	Sec1-like

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