GR407503

EST Overview


Name	GR407503
Unique Name	GR407503
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	418

Libraries


Library Name	Type
LIBEST_024786 JG11 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR407503 vs. SwissProt
Match: CNOT3_HUMAN (CCR4-NOT transcription complex subunit 3 OS=Homo sapiens GN=CNOT3 PE=1 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 1.517e-27
Identity = 62/92 (67.39%), Postives = 71/92 (77.17%), Query Frame = 3

Query:  141 MGASRKLQGEIDRVLKKVQEGVEVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 416
            M   RKLQGEIDR LKKV EGVE F+ IW K+++  NANQKEK+EADLKKEIKKLQR RDQIKTW+ S+EIKDK+        L+D RKLIE
Sbjct:    1 MADKRKLQGEIDRCLKKVSEGVEQFEDIWQKLHNAANANQKEKYEADLKKEIKKLQRLRDQIKTWVASNEIKDKR-------QLIDNRKLIE 85

[Close]

BLAST of GR407503 vs. SwissProt
Match: CNOT3_MOUSE (CCR4-NOT transcription complex subunit 3 OS=Mus musculus GN=Cnot3 PE=1 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 4.414e-27
Identity = 61/92 (66.30%), Postives = 71/92 (77.17%), Query Frame = 3

Query:  141 MGASRKLQGEIDRVLKKVQEGVEVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 416
            M   RKLQGEIDR LKKV EGVE F+ IW K+++  NANQKEK+EADLKKEIKKLQR RDQIKTW+ S+EIKDK+        L++ RKLIE
Sbjct:    1 MADKRKLQGEIDRCLKKVSEGVEQFEDIWQKLHNAANANQKEKYEADLKKEIKKLQRLRDQIKTWVASNEIKDKR-------QLIENRKLIE 85

[Close]

BLAST of GR407503 vs. SwissProt
Match: NOT5_YEAST (General negative regulator of transcription subunit 5 OS=Saccharomyces cerevisiae GN=NOT5 PE=1 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.986e-19
Identity = 47/92 (51.09%), Postives = 67/92 (72.83%), Query Frame = 3

Query:  147 ASRKLQGEIDRVLKKVQEGVEVFDSIWNKVYDTD--NANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 416
            + RKLQ +ID++LKKV+EG+E FD I+ K   TD  N++ +EK E+DLK+EIKKLQ++RDQIKTW+   ++KDK      +  L+  R+LIE
Sbjct:    2 SQRKLQQDIDKLLKKVKEGIEDFDDIYEKFQSTDPSNSSHREKLESDLKREIKKLQKHRDQIKTWLSKEDVKDK------QSVLMTNRRLIE 87

[Close]

BLAST of GR407503 vs. SwissProt
Match: NOT3_YEAST (General negative regulator of transcription subunit 3 OS=Saccharomyces cerevisiae GN=NOT3 PE=1 SV=2)

HSP 1 Score: 87.8113 bits (216), Expect = 1.859e-17
Identity = 45/91 (49.45%), Postives = 65/91 (71.43%), Query Frame = 3

Query:  147 ASRKLQGEIDRVLKKVQEGVEVFDSIWNKVYD-TDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 416
            A RKLQ E+DRV KK+ EG+E+F+S + +    T+N +QK+K E+DLK+E+KKLQR R+QIK+W  S +IKDK        +L+D R+ +E
Sbjct:    2 AHRKLQQEVDRVFKKINEGLEIFNSYYERHESCTNNPSQKDKLESDLKREVKKLQRLREQIKSWQSSPDIKDK-------DSLLDYRRSVE 85

[Close]

BLAST of GR407503 vs. SwissProt
Match: NOT3_SCHPO (General negative regulator of transcription subunit 3 OS=Schizosaccharomyces pombe GN=not3 PE=1 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.628e-13
Identity = 37/73 (50.68%), Postives = 52/73 (71.23%), Query Frame = 3

Query:  198 EGVEVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 416
            E + +FD ++ K+  +++ +QKEK E DLK +IKKLQR RDQIKTW  S++IKDKK       AL++ R+LIE
Sbjct:    9 EKIAIFDEVYEKLSASNSVSQKEKLEGDLKTQIKKLQRLRDQIKTWASSNDIKDKK-------ALLENRRLIE 74

[Close]

BLAST of GR407503 vs. TrEMBL
Match: A2Q5Q7_MEDTR (Not CCR4-Not complex component, N-terminal; tRNA-binding arm OS=Medicago truncatula GN=MtrDRAFT_AC167711g22v2 PE=4 SV=1)

HSP 1 Score: 182.57 bits (462), Expect = 1.125e-44
Identity = 92/92 (100.00%), Postives = 92/92 (100.00%), Query Frame = 3

Query:  141 MGASRKLQGEIDRVLKKVQEGVEVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 416
            MGASRKLQGEIDRVLKKVQEGVEVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE
Sbjct:    1 MGASRKLQGEIDRVLKKVQEGVEVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 92

[Close]

BLAST of GR407503 vs. TrEMBL
Match: B9SHH5_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1121820 PE=4 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 2.506e-44
Identity = 91/92 (98.91%), Postives = 92/92 (100.00%), Query Frame = 3

Query:  141 MGASRKLQGEIDRVLKKVQEGVEVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 416
            MGASRKLQGEIDRVLKKVQEGV+VFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE
Sbjct:    1 MGASRKLQGEIDRVLKKVQEGVDVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 92

[Close]

BLAST of GR407503 vs. TrEMBL
Match: D7TI48_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00033382001 PE=4 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 5.583e-44
Identity = 90/92 (97.83%), Postives = 92/92 (100.00%), Query Frame = 3

Query:  141 MGASRKLQGEIDRVLKKVQEGVEVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 416
            MGASRKLQGEIDRVLKKVQEGV+VFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQAL+DARKLIE
Sbjct:    1 MGASRKLQGEIDRVLKKVQEGVDVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALLDARKLIE 92

[Close]

BLAST of GR407503 vs. TrEMBL
Match: A5BTD7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_041711 PE=4 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 5.583e-44
Identity = 90/92 (97.83%), Postives = 92/92 (100.00%), Query Frame = 3

Query:  141 MGASRKLQGEIDRVLKKVQEGVEVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 416
            MGASRKLQGEIDRVLKKVQEGV+VFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQAL+DARKLIE
Sbjct:    1 MGASRKLQGEIDRVLKKVQEGVDVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALLDARKLIE 92

[Close]

BLAST of GR407503 vs. TrEMBL
Match: B9IPN0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_738421 PE=4 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 9.523e-44
Identity = 90/92 (97.83%), Postives = 91/92 (98.91%), Query Frame = 3

Query:  141 MGASRKLQGEIDRVLKKVQEGVEVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 416
            MGASRKLQGEIDRVLKKVQEGV+VFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARK IE
Sbjct:    1 MGASRKLQGEIDRVLKKVQEGVDVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKTIE 92

[Close]

BLAST of GR407503 vs. TrEMBL
Match: Q9FK48_ARATH (Similarity to transcription regulator OS=Arabidopsis thaliana PE=4 SV=1)

HSP 1 Score: 178.718 bits (452), Expect = 1.624e-43
Identity = 89/92 (96.74%), Postives = 91/92 (98.91%), Query Frame = 3

Query:  141 MGASRKLQGEIDRVLKKVQEGVEVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 416
            MGASRKLQGEIDRVLKKVQEGV+VFDSIWNKVYDTDN NQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQ+LVDARKLIE
Sbjct:    1 MGASRKLQGEIDRVLKKVQEGVDVFDSIWNKVYDTDNVNQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQSLVDARKLIE 92

[Close]

BLAST of GR407503 vs. TrEMBL
Match: Q94JY7_ARATH (Putative uncharacterized protein At5g18230 OS=Arabidopsis thaliana GN=MRG7.18 PE=2 SV=1)

HSP 1 Score: 178.718 bits (452), Expect = 1.624e-43
Identity = 89/92 (96.74%), Postives = 91/92 (98.91%), Query Frame = 3

Query:  141 MGASRKLQGEIDRVLKKVQEGVEVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 416
            MGASRKLQGEIDRVLKKVQEGV+VFDSIWNKVYDTDN NQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQ+LVDARKLIE
Sbjct:    1 MGASRKLQGEIDRVLKKVQEGVDVFDSIWNKVYDTDNVNQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQSLVDARKLIE 92

[Close]

BLAST of GR407503 vs. TrEMBL
Match: D7LXS9_ARALY (Transcription regulator NOT2/NOT3/NOT5 family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_488691 PE=4 SV=1)

HSP 1 Score: 178.718 bits (452), Expect = 1.624e-43
Identity = 89/92 (96.74%), Postives = 91/92 (98.91%), Query Frame = 3

Query:  141 MGASRKLQGEIDRVLKKVQEGVEVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 416
            MGASRKLQGEIDRVLKKVQEGV+VFDSIWNKVYDTDN NQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQ+LVDARKLIE
Sbjct:    1 MGASRKLQGEIDRVLKKVQEGVDVFDSIWNKVYDTDNVNQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQSLVDARKLIE 92

[Close]

BLAST of GR407503 vs. TrEMBL
Match: B9I5Z4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_823712 PE=4 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 2.771e-43
Identity = 89/92 (96.74%), Postives = 90/92 (97.83%), Query Frame = 3

Query:  141 MGASRKLQGEIDRVLKKVQEGVEVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 416
            MGASRKLQGEIDRVLKKVQEGV+VFDSIWNKVYDTDN NQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARK IE
Sbjct:    1 MGASRKLQGEIDRVLKKVQEGVDVFDSIWNKVYDTDNVNQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKTIE 92

[Close]

BLAST of GR407503 vs. TrEMBL
Match: C0Z233_ARATH (AT5G18230 protein (Fragment) OS=Arabidopsis thaliana GN=AT5G18230 PE=2 SV=1)

HSP 1 Score: 173.711 bits (439), Expect = 5.225e-42
Identity = 89/94 (94.68%), Postives = 91/94 (96.81%), Query Frame = 3

Query:  141 MGASRKLQGEIDRVLKKVQEGVEVFDSIWNK--VYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 416
            MGASRKLQGEIDRVLKKVQEGV+VFDSIWNK  VYDTDN NQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQ+LVDARKLIE
Sbjct:    1 MGASRKLQGEIDRVLKKVQEGVDVFDSIWNKWNVYDTDNVNQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQSLVDARKLIE 94

[Close]

BLAST of GR407503 vs. TAIR peptide
Match: AT5G18230.1 (| Symbols: | transcription regulator NOT2/NOT3/NOT5 family protein | chr5:6021610-6027031 REVERSE LENGTH=843)

HSP 1 Score: 178.718 bits (452), Expect = 9.559e-46
Identity = 89/92 (96.74%), Postives = 91/92 (98.91%), Query Frame = 3

Query:  141 MGASRKLQGEIDRVLKKVQEGVEVFDSIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 416
            MGASRKLQGEIDRVLKKVQEGV+VFDSIWNKVYDTDN NQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQ+LVDARKLIE
Sbjct:    1 MGASRKLQGEIDRVLKKVQEGVDVFDSIWNKVYDTDNVNQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQSLVDARKLIE 92

[Close]

BLAST of GR407503 vs. TAIR peptide
Match: AT5G18230.2 (| Symbols: | transcription regulator NOT2/NOT3/NOT5 family protein | chr5:6021610-6027031 REVERSE LENGTH=845)

HSP 1 Score: 173.711 bits (439), Expect = 3.075e-44
Identity = 89/94 (94.68%), Postives = 91/94 (96.81%), Query Frame = 3

Query:  141 MGASRKLQGEIDRVLKKVQEGVEVFDSIWNK--VYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIE 416
            MGASRKLQGEIDRVLKKVQEGV+VFDSIWNK  VYDTDN NQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQ+LVDARKLIE
Sbjct:    1 MGASRKLQGEIDRVLKKVQEGVDVFDSIWNKWNVYDTDNVNQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQSLVDARKLIE 94

The following BLAST results are available for this feature:

BLAST of GR407503 vs. SwissProt
BLAST of GR407503 vs. TrEMBL
BLAST of GR407503 vs. TAIR peptide

BLAST of GR407503 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 5


Match Name	E-value	Identity	Description
CNOT3_HUMAN	1.517e-27	67.39	CCR4-NOT transcription complex subunit 3 OS=Homo s...	[more]
CNOT3_MOUSE	4.414e-27	66.30	CCR4-NOT transcription complex subunit 3 OS=Mus mu...	[more]
NOT5_YEAST	1.986e-19	51.09	General negative regulator of transcription subuni...	[more]
NOT3_YEAST	1.859e-17	49.45	General negative regulator of transcription subuni...	[more]
NOT3_SCHPO	1.628e-13	50.68	General negative regulator of transcription subuni...	[more]

BLAST of GR407503 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A2Q5Q7_MEDTR	1.125e-44	100.00	Not CCR4-Not complex component, N-terminal; tRNA-b...	[more]
B9SHH5_RICCO	2.506e-44	98.91	Putative uncharacterized protein OS=Ricinus commun...	[more]
D7TI48_VITVI	5.583e-44	97.83	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5BTD7_VITVI	5.583e-44	97.83	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9IPN0_POPTR	9.523e-44	97.83	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q9FK48_ARATH	1.624e-43	96.74	Similarity to transcription regulator OS=Arabidops...	[more]
Q94JY7_ARATH	1.624e-43	96.74	Putative uncharacterized protein At5g18230 OS=Arab...	[more]
D7LXS9_ARALY	1.624e-43	96.74	Transcription regulator NOT2/NOT3/NOT5 family prot...	[more]
B9I5Z4_POPTR	2.771e-43	96.74	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
C0Z233_ARATH	5.225e-42	94.68	AT5G18230 protein (Fragment) OS=Arabidopsis thalia...	[more]

BLAST of GR407503 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G18230.1	9.559e-46	96.74	\| Symbols: \| transcription regulator NOT2/NOT3/NO...	[more]
AT5G18230.2	3.075e-44	94.68	\| Symbols: \| transcription regulator NOT2/NOT3/NO...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 24..45 score
None	No IPR available	PANTHER	PTHR23326	CCR4 NOT-RELATED	coord: 4..94 score: 5.9
None	No IPR available	SEG	seg	seg	coord: 27..54 score
IPR007207	Not CCR4-Not complex component, N-terminal	PFAM	PF04065	Not3	coord: 5..92 score: 3.5

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR407503 ID=GR407503; Name=GR407503; organism=Cicer arietinum; type=EST; length=418bp
GAGAATCTCCGCGTCTTCTAGCAAACGAGTGTGAGAGCATAAACAAACCC
TAAAACCAGAATCGAAGGAAAACAATAAAACTAACTGAACATTCATAATC
GATAATAATCAACACTTCAAGAATTGAATGATAATCAAAAATGGGTGCGA
GTAGAAAGCTTCAAGGAGAGATAGATCGCGTTCTCAAGAAGGTTCAAGAA
GGCGTTGAAGTCTTCGATAGTATCTGGAACAAGGTTTACGATACGGACAA
TGCAAACCAGAAGGAGAAATTCGAGGCAGACCTCAAGAAGGAGATTAAGA
AGCTCCAGAGGTATAGAGATCAAATCAAGACTTGGATTCAATCCAGTGAG
ATAAAAGATAAGAAGGTTAGTGCCTCGTATGAGCAGGCTCTGGTGGATGC
GCGGAAGCTAATTGAACG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **Molecular Function**
Term	Definition
GO:0030528	obsolete transcription regulator activity

Vocabulary: **Biological Process**
Term	Definition
GO:0045449	regulation of transcription

Vocabulary: **INTERPRO**
Term	Definition
IPR007207	Not_N

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