CK149102

EST Overview


Name	CK149102
Unique Name	CK149102
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	791

Libraries


Library Name	Type
LIBEST_014674 Suppressive subtractive hybridization library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of CK149102 vs. SwissProt
Match: TRBP2_ARATH (Tryptophan synthase beta chain 2, chloroplastic OS=Arabidopsis thaliana GN=TSB2 PE=2 SV=2)

HSP 1 Score: 325.094 bits (832), Expect = 2.784e-88
Identity = 163/198 (82.32%), Postives = 175/198 (88.38%), Query Frame = 1

Query:  187 VTKDPSSVLPLLDHTKLDNGS-VVHQRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            + KDP+ V+   +  K       + QRPDSFGRFGKFGGKYVPETLMHAL+ELE +FYSL  DEDFQRELA ILKDYVGRESPLYFAERLTEHY+R NGEGP IYLKREDLNHTGAHKINNAVAQALLAK LGKKR+IAETGAGQHGVATATVCARFGL+CIIYMGAQDMERQ+LNVFRMRLLGAEVR VH+GTATLK
Sbjct:   53 IAKDPAVVMADSEKIKAAGSDPTMWQRPDSFGRFGKFGGKYVPETLMHALSELETAFYSLATDEDFQRELAEILKDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLK 250

[Close]

BLAST of CK149102 vs. SwissProt
Match: TRPB1_ARATH (Tryptophan synthase beta chain 1, chloroplastic OS=Arabidopsis thaliana GN=TSB1 PE=2 SV=1)

HSP 1 Score: 324.324 bits (830), Expect = 4.749e-88
Identity = 158/173 (91.33%), Postives = 167/173 (96.53%), Query Frame = 1

Query:  259 QRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            QRPDSFGRFGKFGGKYVPETLMHAL+ELE++FY+L  D+DFQRELAGILKDYVGRESPLYFAERLTEHY+R NGEGP IYLKREDLNHTGAHKINNAVAQALLAK LGKKR+IAETGAGQHGVATATVCARFGLECIIYMGAQDMERQ+LNVFRMRLLGAEVR VH+GTATLK
Sbjct:   73 QRPDSFGRFGKFGGKYVPETLMHALSELESAFYALATDDDFQRELAGILKDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLK 245

[Close]

BLAST of CK149102 vs. SwissProt
Match: TRPB_CAMAC (Tryptophan synthase beta chain 2, chloroplastic OS=Camptotheca acuminata GN=TSB PE=2 SV=1)

HSP 1 Score: 316.235 bits (809), Expect = 1.293e-85
Identity = 155/175 (88.57%), Postives = 166/175 (94.86%), Query Frame = 1

Query:  253 VHQRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            V QRPDSFGRFGKFGGKYVPETLM+ALTELE++F SL+ D+ FQ+EL GILKDYVGRESPLYFAERLT HYKRPNGEGP IYLKREDLNHTGAHKINNAVAQALLAK LGKKR+IAETGAGQHGVATATVCARFGL+C+IYMGAQDMERQ+LNVFRMRLLGAEVR VH+GTATLK
Sbjct:   67 VLQRPDSFGRFGKFGGKYVPETLMYALTELESAFRSLSGDQVFQKELDGILKDYVGRESPLYFAERLTLHYKRPNGEGPEIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLQCVIYMGAQDMERQALNVFRMRLLGAEVRAVHSGTATLK 241

[Close]

BLAST of CK149102 vs. SwissProt
Match: TRPB2_MAIZE (Tryptophan synthase beta chain 2, chloroplastic (Fragment) OS=Zea mays GN=TSB2 PE=2 SV=1)

HSP 1 Score: 306.99 bits (785), Expect = 7.847e-83
Identity = 148/173 (85.55%), Postives = 163/173 (94.22%), Query Frame = 1

Query:  259 QRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            QRPD+ GRFG+FGGKYVPETLMHALTELE++F++L  D++FQ+EL GILKDYVGRESPLYFAERLTEHYKR +G GP IYLKREDLNHTGAHKINNAVAQALLAK LGK+R+IAETGAGQHGVATATVC RFGL+CIIYMGAQDMERQ+LNVFRMRLLGAEVR VH+GTATLK
Sbjct:   46 QRPDAMGRFGRFGGKYVPETLMHALTELESAFHALATDDEFQKELDGILKDYVGRESPLYFAERLTEHYKRADGTGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKQRIIAETGAGQHGVATATVCRRFGLQCIIYMGAQDMERQALNVFRMRLLGAEVRAVHSGTATLK 218

[Close]

BLAST of CK149102 vs. SwissProt
Match: TRPB1_MAIZE (Tryptophan synthase beta chain 1 (Fragment) OS=Zea mays GN=TSB1 PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.888e-77
Identity = 140/164 (85.37%), Postives = 154/164 (93.90%), Query Frame = 1

Query:  286 GKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            G+FGGKYVPETLMHALTELE +F++L  D++FQ+EL GILKDYVGRESPLYFAERLTEHYKR +G GP IYLKREDLNH GAHKINNAVAQALLAK LGK+R+IAETGAGQHGVATATVCARFGL+CIIYMGAQDMERQ+LNVFRM+LLGAEVR VH+GTATLK
Sbjct:    1 GRFGGKYVPETLMHALTELENAFHALATDDEFQKELDGILKDYVGRESPLYFAERLTEHYKRADGTGPLIYLKREDLNHRGAHKINNAVAQALLAKRLGKQRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMKLLGAEVRAVHSGTATLK 164

[Close]

BLAST of CK149102 vs. SwissProt
Match: TRPB_SYNY3 (Tryptophan synthase beta chain OS=Synechocystis sp. (strain PCC 6803) GN=trpB PE=3 SV=1)

HSP 1 Score: 280.411 bits (716), Expect = 7.865e-75
Identity = 136/174 (78.16%), Postives = 148/174 (85.06%), Query Frame = 1

Query:  256 HQRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            HQ PD+ GRFG +GGKYVPETLM ALTELE ++Y   A+  FQ ELAG+LKDYVGR SPLYFAERL+ HY RP+G  P IYLKREDLNHTGAHKINNA+ Q LLAK +GKKR+IAETGAGQHGVATATVCARFGLECIIYMG QDMERQ LNVFRM LLGA V+PV AGT TLK
Sbjct:   12 HQYPDALGRFGNYGGKYVPETLMPALTELEEAYYRYRAESSFQEELAGLLKDYVGRSSPLYFAERLSAHYARPDGTYPLIYLKREDLNHTGAHKINNALGQVLLAKRMGKKRIIAETGAGQHGVATATVCARFGLECIIYMGVQDMERQKLNVFRMNLLGARVQPVTAGTGTLK 185

[Close]

BLAST of CK149102 vs. SwissProt
Match: TRPB1_ANASP (Tryptophan synthase beta chain 1 OS=Anabaena sp. (strain PCC 7120) GN=trpB1 PE=3 SV=1)

HSP 1 Score: 271.937 bits (694), Expect = 2.797e-72
Identity = 131/172 (76.16%), Postives = 147/172 (85.47%), Query Frame = 1

Query:  262 RPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            RPD+ GRFGKFGGKYVPETLM AL ELE ++     D  +Q EL  +L+DYVGR SPLYFAERLTE+Y RP+G G  IYLKREDLNHTGAHKINNA+AQ LLAK +GK+RVIAETGAGQHGVATATVCARFGL+C+IYMG  DMERQ+LNVFRM+L+GAEVRPV AGT TLK
Sbjct:   13 RPDALGRFGKFGGKYVPETLMPALGELETAYQKYRDDASYQTELQNLLRDYVGRPSPLYFAERLTEYYARPDGTGAQIYLKREDLNHTGAHKINNALAQVLLAKRIGKQRVIAETGAGQHGVATATVCARFGLDCVIYMGIHDMERQALNVFRMKLMGAEVRPVEAGTGTLK 184

[Close]

BLAST of CK149102 vs. SwissProt
Match: TRPB_TRIEI (Tryptophan synthase beta chain OS=Trichodesmium erythraeum (strain IMS101) GN=trpB PE=3 SV=1)

HSP 1 Score: 271.552 bits (693), Expect = 3.653e-72
Identity = 130/173 (75.14%), Postives = 149/173 (86.13%), Query Frame = 1

Query:  259 QRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            Q+PDS GRFGKFGGKYVPETLM AL +LE +    + D  F++EL  +L+DYVGR SPLYFAERLT HY +P+G GP IYLKREDLNHTGAHKINNA+ QALLA+ +GK+R+IAETGAGQHGVATATVCARFGL+C+IYMG  DMERQ+LNVFRMRL+GAEVRPV AGT TLK
Sbjct:   12 QQPDSLGRFGKFGGKYVPETLMPALLQLETACKKYSQDPTFKQELQQLLRDYVGRPSPLYFAERLTAHYTKPDGTGPQIYLKREDLNHTGAHKINNALGQALLAQRMGKQRIIAETGAGQHGVATATVCARFGLKCVIYMGIHDMERQALNVFRMRLMGAEVRPVEAGTGTLK 184

[Close]

BLAST of CK149102 vs. SwissProt
Match: TRPB_ANAVT (Tryptophan synthase beta chain OS=Anabaena variabilis (strain ATCC 29413 / PCC 7937) GN=trpB PE=3 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 5.276e-71
Identity = 130/173 (75.14%), Postives = 144/173 (83.24%), Query Frame = 1

Query:  259 QRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            Q PD  GRFG+FGGKYVPETLM AL ELE ++     D  FQ EL  +L+DYVGR +PLYFAERLT HY RP+G G  IYLKREDLNHTGAHKINNA+ Q LLAK +GK+R+IAETGAGQHGVATATVCARFGLEC+IYMG  DMERQ+LNVFRMRL+GAEVRPV AGT TLK
Sbjct:   15 QVPDIQGRFGRFGGKYVPETLMPALAELETAYQKYRHDPGFQAELQQLLRDYVGRATPLYFAERLTAHYARPDGTGAQIYLKREDLNHTGAHKINNALGQVLLAKRMGKQRIIAETGAGQHGVATATVCARFGLECVIYMGVHDMERQALNVFRMRLMGAEVRPVEAGTGTLK 187

[Close]

BLAST of CK149102 vs. SwissProt
Match: TRPB2_ANASP (Tryptophan synthase beta chain 2 OS=Anabaena sp. (strain PCC 7120) GN=trpB2 PE=3 SV=1)

HSP 1 Score: 266.929 bits (681), Expect = 8.999e-71
Identity = 130/173 (75.14%), Postives = 144/173 (83.24%), Query Frame = 1

Query:  259 QRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            Q PD  GRFG+FGGKYVPETLM AL ELE ++     D  FQ EL  +L+DYVGR +PLYFAERLT HY RP+G G  IYLKREDLNHTGAHKINNA+ Q LLAK +GK+R+IAETGAGQHGVATATVCARFGLEC+IYMG  DMERQ+LNVFRMRL+GAEVRPV AGT TLK
Sbjct:   15 QVPDIQGRFGRFGGKYVPETLMPALAELETAYQQYRNDPGFQAELQQLLRDYVGRATPLYFAERLTAHYARPDGTGAQIYLKREDLNHTGAHKINNALGQVLLAKRMGKQRIIAETGAGQHGVATATVCARFGLECVIYMGVHDMERQALNVFRMRLMGAEVRPVAAGTGTLK 187

[Close]

BLAST of CK149102 vs. TrEMBL
Match: Q0WS13_ARATH (Tryptophan synthase beta-subunit OS=Arabidopsis thaliana GN=At4g27070 PE=2 SV=1)

HSP 1 Score: 325.094 bits (832), Expect = 4.297e-87
Identity = 163/198 (82.32%), Postives = 175/198 (88.38%), Query Frame = 1

Query:  187 VTKDPSSVLPLLDHTKLDNGS-VVHQRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            + KDP+ V+   +  K       + QRPDSFGRFGKFGGKYVPETLMHAL+ELE +FYSL  DEDFQRELA ILKDYVGRESPLYFAERLTEHY+R NGEGP IYLKREDLNHTGAHKINNAVAQALLAK LGKKR+IAETGAGQHGVATATVCARFGL+CIIYMGAQDMERQ+LNVFRMRLLGAEVR VH+GTATLK
Sbjct:   53 IAKDPAVVMADSEKIKAAGSDPTMWQRPDSFGRFGKFGGKYVPETLMHALSELETAFYSLATDEDFQRELAEILKDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLK 250

[Close]

BLAST of CK149102 vs. TrEMBL
Match: Q0WUI8_ARATH (Tryptophan synthase beta chain 1 OS=Arabidopsis thaliana GN=At5g54810 PE=2 SV=1)

HSP 1 Score: 324.324 bits (830), Expect = 7.330e-87
Identity = 158/173 (91.33%), Postives = 167/173 (96.53%), Query Frame = 1

Query:  259 QRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            QRPDSFGRFGKFGGKYVPETLMHAL+ELE++FY+L  D+DFQRELAGILKDYVGRESPLYFAERLTEHY+R NGEGP IYLKREDLNHTGAHKINNAVAQALLAK LGKKR+IAETGAGQHGVATATVCARFGLECIIYMGAQDMERQ+LNVFRMRLLGAEVR VH+GTATLK
Sbjct:   73 QRPDSFGRFGKFGGKYVPETLMHALSELESAFYALATDDDFQRELAGILKDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLK 245

[Close]

BLAST of CK149102 vs. TrEMBL
Match: D7MUW5_ARALY (Tryptophan synthase beta-subunit OS=Arabidopsis lyrata subsp. lyrata GN=TSB1 PE=4 SV=1)

HSP 1 Score: 324.324 bits (830), Expect = 7.330e-87
Identity = 158/173 (91.33%), Postives = 167/173 (96.53%), Query Frame = 1

Query:  259 QRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            QRPDSFGRFGKFGGKYVPETLMHAL+ELE++FY+L  D+DFQRELAGILKDYVGRESPLYFAERLTEHY+R NGEGP IYLKREDLNHTGAHKINNAVAQALLAK LGKKR+IAETGAGQHGVATATVCARFGLECIIYMGAQDMERQ+LNVFRMRLLGAEVR VH+GTATLK
Sbjct:   74 QRPDSFGRFGKFGGKYVPETLMHALSELESAFYALATDDDFQRELAGILKDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLK 246

[Close]

BLAST of CK149102 vs. TrEMBL
Match: D7MEJ4_ARALY (Tryptophan synthase beta-subunit OS=Arabidopsis lyrata subsp. lyrata GN=TSB2 PE=4 SV=1)

HSP 1 Score: 323.939 bits (829), Expect = 9.573e-87
Identity = 162/197 (82.23%), Postives = 174/197 (88.32%), Query Frame = 1

Query:  187 VTKDPSSVLPLLDHTKLDNGSVVHQRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            + KDP+  +   D     +   + QRPDSFGRFGKFGGKYVPETLMHAL+ELE +FYSL  DEDFQRELA ILKDYVGRESPLYFAERLTEHY+R NGEGP IYLKREDLNHTGAHKINNAVAQALLAK LGKKR+IAETGAGQHGVATATVCARFGL+CIIYMGAQDMERQ+LNVFRMRLLGAEVR VH+GTATLK
Sbjct:   52 IAKDPTVFMA--DTEAAGSDPTMWQRPDSFGRFGKFGGKYVPETLMHALSELETAFYSLATDEDFQRELAEILKDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLK 246

[Close]

BLAST of CK149102 vs. TrEMBL
Match: Q5KT13_9CARY (Tryptophan synthase beta subunit OS=Persicaria tinctoria PE=2 SV=1)

HSP 1 Score: 319.701 bits (818), Expect = 1.805e-85
Identity = 171/258 (66.28%), Postives = 187/258 (72.48%), Query Frame = 1

Query:    7 VAAEVTKSNMASSITTLNSSRLLPISKEQSYPSS-HFHSNLLKVXXXXXXXXXXXXXXXXXVTKDPSSVLPLLDHTKLDNGSVVHQRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            V+      +  SS ++L+ SRL       S+P S  F    L                   +  D +   PLL            QRPDSFGRFGKFGGKYVPETLMHAL ELE +F+SL  D +FQ+EL GILKDYVGRE+PLYFAERLTEHYKRPNGEGP IYLKREDLNHTGAHKINNAVAQALLAK LGK R+IAETGAGQHGVATATVCARFGLECI+YMGAQDMERQSLNVFRMRLLGAEVR VH+GTATLK
Sbjct:    3 VSTSSALKSQTSSYSSLSQSRLSAFQFSDSHPKSIRFAQKPLSRCPSISCVMTREDLGAKSLKADAADQEPLL-----------LQRPDSFGRFGKFGGKYVPETLMHALDELETAFHSLATDVEFQKELDGILKDYVGRETPLYFAERLTEHYKRPNGEGPQIYLKREDLNHTGAHKINNAVAQALLAKRLGKTRIIAETGAGQHGVATATVCARFGLECIVYMGAQDMERQSLNVFRMRLLGAEVRGVHSGTATLK 249

[Close]

BLAST of CK149102 vs. TrEMBL
Match: B9I7M4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_773968 PE=4 SV=1)

HSP 1 Score: 314.309 bits (804), Expect = 7.585e-84
Identity = 150/181 (82.87%), Postives = 166/181 (91.71%), Query Frame = 1

Query:  235 LDNGSVVHQRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            +D+     QRPDSFGRFGKFGGKYVPETL+HALTELE++F+SL  D +F++EL GILKDYVGRE+PLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNA+ Q LLAK LGK+R+IAETGAGQHGVATATVCARFG  C++YMGAQDMERQSLNVFRMRLLGAEVR VH+GTATLK
Sbjct:    5 VDSDPAQWQRPDSFGRFGKFGGKYVPETLIHALTELESAFHSLKDDPEFKKELDGILKDYVGRENPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAIGQVLLAKRLGKQRIIAETGAGQHGVATATVCARFGFPCVVYMGAQDMERQSLNVFRMRLLGAEVRGVHSGTATLK 185

[Close]

BLAST of CK149102 vs. TrEMBL
Match: B9SAI8_RICCO (Tryptophan synthase beta chain, putative OS=Ricinus communis GN=RCOM_0587660 PE=4 SV=1)

HSP 1 Score: 313.923 bits (803), Expect = 9.907e-84
Identity = 154/197 (78.17%), Postives = 176/197 (89.34%), Query Frame = 1

Query:  187 VTKDPSSVLPLLDHTKLDNGSVVHQRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            +T++ S+V   ++  K  +   + QRPDSFGRFG+FGGKYVPETLM+AL+ELE++F+SL  D DFQ+EL GILKDYVGRE+PLYFAERLTEHY RPNGEGPHIYLKREDLNHTGAHKINNA+ Q LLAK LGK+R+IAETGAGQHGVATATVCARFGL+CIIYMGAQDMERQ+LNVFRMRLLGAEVR VH+GTATLK
Sbjct:   56 LTREASAVK--MEEEKEGSDPALWQRPDSFGRFGQFGGKYVPETLMYALSELESAFHSLKDDLDFQKELDGILKDYVGRETPLYFAERLTEHYGRPNGEGPHIYLKREDLNHTGAHKINNAIGQVLLAKRLGKERIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMRLLGAEVRAVHSGTATLK 250

[Close]

BLAST of CK149102 vs. TrEMBL
Match: Q8W0T4_SORBI (Putative uncharacterized protein Sb07g002770 OS=Sorghum bicolor GN=Sb07g002770 PE=4 SV=1)

HSP 1 Score: 311.612 bits (797), Expect = 4.917e-83
Identity = 154/184 (83.70%), Postives = 169/184 (91.85%), Query Frame = 1

Query:  232 KLDNGSVVH--QRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            ++ NG+ V   QRPD+ GRFGKFGGKYVPETLMHALTELE +F++L  DE+FQ+EL GILKDYVGRESPLYFAERLTEHYKR +G GP IYLKREDLNHTGAHKINNAVAQALLAK LGK+R+IAETGAGQHGVATATVCARFGL+CIIYMGAQDMERQ+LNVFRMRLLGAEVR VH+GTATLK
Sbjct:   68 EMGNGAAVAGMQRPDAMGRFGKFGGKYVPETLMHALTELENAFHALATDEEFQKELDGILKDYVGRESPLYFAERLTEHYKRADGTGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKQRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMRLLGAEVRAVHSGTATLK 251

[Close]

BLAST of CK149102 vs. TrEMBL
Match: D7T0C5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_177.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00001997001 PE=4 SV=1)

HSP 1 Score: 311.612 bits (797), Expect = 4.917e-83
Identity = 153/173 (88.44%), Postives = 163/173 (94.22%), Query Frame = 1

Query:  259 QRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            Q PDSFGRFGKFGGKYVPETLMHALTELE++F +L+ D +FQ+EL GILKDYVGRESPLYFAERLTEHYKR NGEGP IYLKREDLNHTGAHKINNAVAQALLAK LGK R+IAETGAGQHGVATATVCARFGL+CI+YMGAQDMERQ+LNVFRMRLLGAEVR VHAGTATLK
Sbjct:   10 QVPDSFGRFGKFGGKYVPETLMHALTELESAFRALSGDVEFQKELDGILKDYVGRESPLYFAERLTEHYKRHNGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKTRIIAETGAGQHGVATATVCARFGLQCIVYMGAQDMERQALNVFRMRLLGAEVRAVHAGTATLK 182

[Close]

BLAST of CK149102 vs. TrEMBL
Match: B9I0B9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_888009 PE=4 SV=1)

HSP 1 Score: 311.612 bits (797), Expect = 4.917e-83
Identity = 148/180 (82.22%), Postives = 166/180 (92.22%), Query Frame = 1

Query:  238 DNGSVVHQRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            D+   + QRPDSFGRFGK+GGKYVPETLM+ALTELE++FYS   D +F++E+ GILKDYVGRE+PLYFAERLTEHYKRP+GEGPHIYLKREDLNHTGAHKINNAV Q LLAK LGK+R+IAETGAGQHGVATATVCARFGL C++YMGAQDMERQ+LNVFRMRLLGAEVR VH+GTATLK
Sbjct:    6 DSDPALWQRPDSFGRFGKYGGKYVPETLMYALTELESAFYSFKDDPEFKKEINGILKDYVGRENPLYFAERLTEHYKRPSGEGPHIYLKREDLNHTGAHKINNAVGQVLLAKRLGKQRIIAETGAGQHGVATATVCARFGLPCVVYMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLK 185

[Close]

BLAST of CK149102 vs. TAIR peptide
Match: AT4G27070.1 (| Symbols: TSB2 | tryptophan synthase beta-subunit 2 | chr4:13586564-13588619 FORWARD LENGTH=475)

HSP 1 Score: 325.094 bits (832), Expect = 2.768e-89
Identity = 163/198 (82.32%), Postives = 175/198 (88.38%), Query Frame = 1

Query:  187 VTKDPSSVLPLLDHTKLDNGS-VVHQRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            + KDP+ V+   +  K       + QRPDSFGRFGKFGGKYVPETLMHAL+ELE +FYSL  DEDFQRELA ILKDYVGRESPLYFAERLTEHY+R NGEGP IYLKREDLNHTGAHKINNAVAQALLAK LGKKR+IAETGAGQHGVATATVCARFGL+CIIYMGAQDMERQ+LNVFRMRLLGAEVR VH+GTATLK
Sbjct:   53 IAKDPAVVMADSEKIKAAGSDPTMWQRPDSFGRFGKFGGKYVPETLMHALSELETAFYSLATDEDFQRELAEILKDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLK 250

[Close]

BLAST of CK149102 vs. TAIR peptide
Match: AT5G54810.1 (| Symbols: TSB1, TRPB, TRP2, ATTSB1 | tryptophan synthase beta-subunit 1 | chr5:22264805-22266738 REVERSE LENGTH=470)

HSP 1 Score: 324.324 bits (830), Expect = 4.721e-89
Identity = 158/173 (91.33%), Postives = 167/173 (96.53%), Query Frame = 1

Query:  259 QRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRPVHAGTATLK 777
            QRPDSFGRFGKFGGKYVPETLMHAL+ELE++FY+L  D+DFQRELAGILKDYVGRESPLYFAERLTEHY+R NGEGP IYLKREDLNHTGAHKINNAVAQALLAK LGKKR+IAETGAGQHGVATATVCARFGLECIIYMGAQDMERQ+LNVFRMRLLGAEVR VH+GTATLK
Sbjct:   73 QRPDSFGRFGKFGGKYVPETLMHALSELESAFYALATDDDFQRELAGILKDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLK 245

[Close]

BLAST of CK149102 vs. TAIR peptide
Match: AT5G28237.1 (| Symbols: | Pyridoxal-5'-phosphate-dependent enzyme family protein | chr5:10207477-10213542 REVERSE LENGTH=465)

HSP 1 Score: 210.69 bits (535), Expect = 7.607e-55
Identity = 107/180 (59.44%), Postives = 129/180 (71.67%), Query Frame = 1

Query:  214 PLLDHTKLDNGSVVHQRPDSFGRFGKFGGKYVPETLMHALTELEASFYSLTADEDFQRELAGILKDYVGRESPLYFAERLTEHYKR--PNGEG-PHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEV 744
            P LD            R D  G+FG+FGGK+VPETLM  L ELE  F  +  D +FQ EL   L+DYVGRE+PLYFAERLTEHYK   P  EG P IYLKREDL+H G+HKINNA+AQA++++ LG  RV+A TGAGQHGVATA  CA+  LEC ++MGA D+E+QS NV  M+LLGA+V
Sbjct:   49 PSLDSVPTRTDKRQFLRGDGNGKFGRFGGKFVPETLMSRLIELEDEFNFVRCDHEFQEELTTALRDYVGRETPLYFAERLTEHYKNIVPTIEGGPEIYLKREDLSHCGSHKINNALAQAMISRRLGCSRVVAATGAGQHGVATAAACAKLSLECTVFMGAADIEKQSFNVLSMKLLGAQV 228

[Close]

BLAST of CK149102 vs. TAIR peptide
Match: AT5G38530.1 (| Symbols: TSBtype2 | tryptophan synthase beta type 2 | chr5:15424097-15426294 FORWARD LENGTH=506)

HSP 1 Score: 64.6994 bits (156), Expect = 6.742e-11
Identity = 44/125 (35.20%), Postives = 61/125 (48.80%), Query Frame = 1

Query:  376 DFQRELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKSLGKKRVIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQSLNVFRMRLLGAEVRP 750
            D   E+  I K  + R +PL  A+RL +  + P      IY K E  +  G+HK N AV QA      G K V+ ETGAGQ G + A   + FGL+C ++  A     +      M+  GA+V P
Sbjct:  119 DIPEEVLEIYK--LWRPTPLIRAKRLEKLLQTP----ARIYFKYEGGSPAGSHKPNTAVPQAYYNAKEGVKNVVTETGAGQWGSSLAFASSLFGLDCEVWQVANSYHTKPYRRLMMQTWGAKVHP 237

The following BLAST results are available for this feature:

BLAST of CK149102 vs. SwissProt
BLAST of CK149102 vs. TrEMBL
BLAST of CK149102 vs. TAIR peptide

BLAST of CK149102 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
TRBP2_ARATH	2.784e-88	82.32	Tryptophan synthase beta chain 2, chloroplastic OS...	[more]
TRPB1_ARATH	4.749e-88	91.33	Tryptophan synthase beta chain 1, chloroplastic OS...	[more]
TRPB_CAMAC	1.293e-85	88.57	Tryptophan synthase beta chain 2, chloroplastic OS...	[more]
TRPB2_MAIZE	7.847e-83	85.55	Tryptophan synthase beta chain 2, chloroplastic (F...	[more]
TRPB1_MAIZE	2.888e-77	85.37	Tryptophan synthase beta chain 1 (Fragment) OS=Zea...	[more]
TRPB_SYNY3	7.865e-75	78.16	Tryptophan synthase beta chain OS=Synechocystis sp...	[more]
TRPB1_ANASP	2.797e-72	76.16	Tryptophan synthase beta chain 1 OS=Anabaena sp. (...	[more]
TRPB_TRIEI	3.653e-72	75.14	Tryptophan synthase beta chain OS=Trichodesmium er...	[more]
TRPB_ANAVT	5.276e-71	75.14	Tryptophan synthase beta chain OS=Anabaena variabi...	[more]
TRPB2_ANASP	8.999e-71	75.14	Tryptophan synthase beta chain 2 OS=Anabaena sp. (...	[more]

BLAST of CK149102 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q0WS13_ARATH	4.297e-87	82.32	Tryptophan synthase beta-subunit OS=Arabidopsis th...	[more]
Q0WUI8_ARATH	7.330e-87	91.33	Tryptophan synthase beta chain 1 OS=Arabidopsis th...	[more]
D7MUW5_ARALY	7.330e-87	91.33	Tryptophan synthase beta-subunit OS=Arabidopsis ly...	[more]
D7MEJ4_ARALY	9.573e-87	82.23	Tryptophan synthase beta-subunit OS=Arabidopsis ly...	[more]
Q5KT13_9CARY	1.805e-85	66.28	Tryptophan synthase beta subunit OS=Persicaria tin...	[more]
B9I7M4_POPTR	7.585e-84	82.87	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9SAI8_RICCO	9.907e-84	78.17	Tryptophan synthase beta chain, putative OS=Ricinu...	[more]
Q8W0T4_SORBI	4.917e-83	83.70	Putative uncharacterized protein Sb07g002770 OS=So...	[more]
D7T0C5_VITVI	4.917e-83	88.44	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9I0B9_POPTR	4.917e-83	82.22	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]

BLAST of CK149102 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT4G27070.1	2.768e-89	82.32	\| Symbols: TSB2 \| tryptophan synthase beta-subunit...	[more]
AT5G54810.1	4.721e-89	91.33	\| Symbols: TSB1, TRPB, TRP2, ATTSB1 \| tryptophan s...	[more]
AT5G28237.1	7.607e-55	59.44	\| Symbols: \| Pyridoxal-5'-phosphate-dependent enz...	[more]
AT5G38530.1	6.742e-11	35.20	\| Symbols: TSBtype2 \| tryptophan synthase beta typ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001926	Pyridoxal phosphate-dependent enzyme, beta subunit	PFAM	PF00291	PALP	coord: 159..252 score: 1.1
IPR001926	Pyridoxal phosphate-dependent enzyme, beta subunit	SUPERFAMILY	53686	Tryptophan synthase beta subunit-like PLP-dependent enzymes	coord: 88..259 score: 1.2
IPR006653	Tryptophan synthase, beta chain, conserved site	PROSITE	PS00168	TRP_SYNTHASE_BETA	coord: 172..186 score
None	No IPR available	GENE3D	3.40.50.1100	no description	coord: 164..259 score: 8.5
None	No IPR available	PANTHER	PTHR10314	SER/THR DEHYDRATASE, TRP SYNTHASE	coord: 102..259 score: 6.8
None	No IPR available	PANTHER	PTHR10314:SF3	TRYPTOPHAN SYNTHASE BETA CHAIN	coord: 102..259 score: 6.8
None	No IPR available	SEG	seg	seg	coord: 46..62 score
None	No IPR available	SEG	seg	seg	coord: 4..26 score: NAcoord: 61..80 score
None	No IPR available	SEG	seg	seg	coord: 31..51 score
None	No IPR available	SEG	seg	seg	coord: 3..20 score
None	No IPR available	SEG	seg	seg	coord: 73..88 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK149102 ID=CK149102; Name=CK149102; organism=Cicer arietinum; type=EST; length=791bp
AGCGTGGTCGCGGCCGAGGTCACCAAATCAAACATGGCTTCATCCATCAC
AACCCTCAACTCATCAAGATTACTTCCCATTTCCAAAGAACAAAGCTACC
CATCTTCCCATTTTCACTCAAACCTTCTAAAGGTTTCATCTTTCCCTTCT
AGAACCTCTTCTTCTTCTTATTCTCTTTCTTGTTCTGTTACTAAAGACCC
TTCCTCTGTTCTTCCATTGCTGGATCATACGAAGCTTGATAATGGGTCGG
TTGTGCATCAACGACCCGATTCGTTTGGGAGGTTTGGGAAATTTGGTGGG
AAATATGTTCCTGAAACTCTTATGCATGCTCTCACTGAACTTGAGGCTTC
TTTCTATTCCCTTACTGCTGATGAAGATTTTCAGAGAGAGCTGGCAGGGA
TTCTAAAGGACTATGTTGGTCGGGAGAGTCCTCTTTATTTTGCGGAAAGG
TTGACGGAGCATTACAAGAGGCCTAATGGTGAAGGGCCTCACATTTACCT
GAAGAGGGAAGATCTTAACCACACCGGTGCTCATAAAATTAACAATGCTG
TTGCACAAGCTTTGCTTGCCAAGAGTTTGGGCAAAAAACGTGTTATTGCT
GAAACCGGAGCTGGACAGCATGGAGTTGCTACTGCTACTGTATGTGCTCG
ATTTGGTTTAGAATGCATTATTTATATGGGTGCACAGGATATGGAAAGGC
AATCACTGAATGTTTTCAGAATGCGTCTTCTTGGTGCTGAGGTGAGACCA
GTCCATGCTGGGACTGCTACACTTAAGATGCTACATCCAGG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0030170	pyridoxal phosphate binding
GO:0004834	tryptophan synthase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process
GO:0006568	tryptophan metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR001926	TrpB-like_PALP
IPR006653	Trp_synth_b_CS

Search form