GR914286

EST Overview


Name	GR914286
Unique Name	GR914286
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	253

Libraries


Library Name	Type
LIBEST_024968 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. JG-62) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28

Homology

[Close]

BLAST of GR914286 vs. TrEMBL
Match: D7TLM3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_19.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00016464001 PE=4 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.111e-23
Identity = 53/76 (69.74%), Postives = 61/76 (80.26%), Query Frame = 1

Query:   25 VPLQVARPLDAKLLNGLTFGLEG*HQYLNAGLVVALCSTWLKRTGHVGDTHFDQTDALPEQFVKGLTNVSLQGRAQ 252
            VPLQV  P+DA LLNGL  GLEG HQYLNAGL +ALCSTWL+RT H+   + +QT +LPEQF+KGLT  SLQGRAQ
Sbjct:  320 VPLQVIPPMDASLLNGLKLGLEGEHQYLNAGLAIALCSTWLQRTSHLEINYLEQTSSLPEQFIKGLTTASLQGRAQ 395

[Close]

BLAST of GR914286 vs. TrEMBL
Match: B9HMM6_POPTR (Folylpolyglutamate synthase OS=Populus trichocarpa GN=FPGS2 PE=4 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 2.096e-22
Identity = 54/76 (71.05%), Postives = 59/76 (77.63%), Query Frame = 1

Query:   25 VPLQVARPLDAKLLNGLTFGLEG*HQYLNAGLVVALCSTWLKRTGHVGDTHFDQTDALPEQFVKGLTNVSLQGRAQ 252
            V LQVA PLDAKLLNGL   LEG HQYLNAGL +AL  TWL+RTGH   TH +Q  +LPEQF+KGLT  SLQGRAQ
Sbjct:  237 VHLQVAEPLDAKLLNGLKLRLEGEHQYLNAGLAIALSFTWLQRTGHHEFTHMEQASSLPEQFIKGLTTASLQGRAQ 312

[Close]

BLAST of GR914286 vs. TrEMBL
Match: B9SE17_RICCO (Folylpolyglutamate synthase, putative OS=Ricinus communis GN=RCOM_1482420 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 5.162e-21
Identity = 50/76 (65.79%), Postives = 58/76 (76.32%), Query Frame = 1

Query:   25 VPLQVARPLDAKLLNGLTFGLEG*HQYLNAGLVVALCSTWLKRTGHVGDTHFDQTDALPEQFVKGLTNVSLQGRAQ 252
            VPLQVA PLD  LLNGL  GLEG HQYLNAGL +AL S WL+RTGHV  ++ +Q+  LPE F++GLT   LQGRAQ
Sbjct:  239 VPLQVAPPLDPSLLNGLKLGLEGEHQYLNAGLAIALSSKWLQRTGHVEFSYLEQSSPLPEHFIRGLTTACLQGRAQ 314

[Close]

BLAST of GR914286 vs. TrEMBL
Match: B9HT54_POPTR (Folylpolyglutamate synthase OS=Populus trichocarpa GN=FPGS3 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 5.162e-21
Identity = 53/76 (69.74%), Postives = 58/76 (76.32%), Query Frame = 1

Query:   25 VPLQVARPLDAKLLNGLTFGLEG*HQYLNAGLVVALCSTWLKRTGHVGDTHFDQTDALPEQFVKGLTNVSLQGRAQ 252
            V L+VA PLDAKLLNGL   LEG HQYLNAGL +AL STWL+RTGH   T  +Q   LPEQF+KGLT  SLQGRAQ
Sbjct:  280 VTLKVAEPLDAKLLNGLKLSLEGEHQYLNAGLAIALSSTWLQRTGHHEFTCPEQASPLPEQFIKGLTTASLQGRAQ 355

[Close]

BLAST of GR914286 vs. TrEMBL
Match: Q10SU1_ORYSJ (FolC bifunctional protein, expressed OS=Oryza sativa subsp. japonica GN=Os03g0111100 PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.718e-16
Identity = 44/76 (57.89%), Postives = 53/76 (69.74%), Query Frame = 1

Query:   25 VPLQVARPLDAKLLNGLTFGLEG*HQYLNAGLVVALCSTWLKRTGHVGDTHFDQTDALPEQFVKGLTNVSLQGRAQ 252
            V LQVA PL+   L     GL G HQY+NAGL VAL STWL++ GHV     ++TD LP+QF+ GL+N SLQGRAQ
Sbjct:  282 VSLQVAHPLEPHQLKDQHLGLRGEHQYVNAGLAVALASTWLEKQGHVERIPLNRTDPLPDQFISGLSNASLQGRAQ 357

[Close]

BLAST of GR914286 vs. TrEMBL
Match: B9FAE3_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_09133 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.718e-16
Identity = 44/76 (57.89%), Postives = 53/76 (69.74%), Query Frame = 1

Query:   25 VPLQVARPLDAKLLNGLTFGLEG*HQYLNAGLVVALCSTWLKRTGHVGDTHFDQTDALPEQFVKGLTNVSLQGRAQ 252
            V LQVA PL+   L     GL G HQY+NAGL VAL STWL++ GHV     ++TD LP+QF+ GL+N SLQGRAQ
Sbjct:  247 VSLQVAHPLEPHQLKDQHLGLRGEHQYVNAGLAVALASTWLEKQGHVERIPLNRTDPLPDQFISGLSNASLQGRAQ 322

[Close]

BLAST of GR914286 vs. TrEMBL
Match: B8ALG2_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_09694 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.718e-16
Identity = 44/76 (57.89%), Postives = 53/76 (69.74%), Query Frame = 1

Query:   25 VPLQVARPLDAKLLNGLTFGLEG*HQYLNAGLVVALCSTWLKRTGHVGDTHFDQTDALPEQFVKGLTNVSLQGRAQ 252
            V LQVA PL+   L     GL G HQY+NAGL VAL STWL++ GHV     ++TD LP+QF+ GL+N SLQGRAQ
Sbjct:  248 VSLQVAHPLEPHQLKDQHLGLRGEHQYVNAGLAVALASTWLEKQGHVERIPLNRTDPLPDQFISGLSNASLQGRAQ 323

[Close]

BLAST of GR914286 vs. TrEMBL
Match: C5WWE5_SORBI (Putative uncharacterized protein Sb01g017810 OS=Sorghum bicolor GN=Sb01g017810 PE=4 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 6.530e-16
Identity = 41/76 (53.95%), Postives = 52/76 (68.42%), Query Frame = 1

Query:   25 VPLQVARPLDAKLLNGLTFGLEG*HQYLNAGLVVALCSTWLKRTGHVGDTHFDQTDALPEQFVKGLTNVSLQGRAQ 252
            V LQV +PLD + L     GL G HQY+NAGL VAL +TWL+R GH+   H   +  LP+QF+KGL++  LQGRAQ
Sbjct:  315 VSLQVVQPLDPQKLEDQPLGLHGEHQYMNAGLAVALANTWLEREGHLNRIHVKHSGTLPDQFIKGLSSACLQGRAQ 390

[Close]

BLAST of GR914286 vs. TrEMBL
Match: B7F7H0_ORYSJ (cDNA clone:J033039A16, full insert sequence OS=Oryza sativa subsp. japonica PE=2 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.114e-15
Identity = 41/77 (53.25%), Postives = 54/77 (70.13%), Query Frame = 1

Query:   22 QVPLQVARPLDAKLLNGLTFGLEG*HQYLNAGLVVALCSTWLKRTGHVGDTHFDQTDALPEQFVKGLTNVSLQGRAQ 252
            +VPLQV +PLD + L+    GL G HQY+NAGL VAL +TWL+R GH    H   +  LP+QF++GL++  LQGRAQ
Sbjct:   23 KVPLQVVQPLDPQQLDDQPLGLHGEHQYMNAGLAVALVNTWLQRQGHFNILHKKHSVTLPDQFIEGLSSACLQGRAQ 99

[Close]

BLAST of GR914286 vs. TrEMBL
Match: Q337F3_ORYSJ (Dihydrofolate synthetase/folylpolyglutamate synthetase, putative, expressed OS=Oryza sativa subsp. japonica GN=Os10g0503200 PE=4 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.455e-15
Identity = 41/76 (53.95%), Postives = 53/76 (69.74%), Query Frame = 1

Query:   25 VPLQVARPLDAKLLNGLTFGLEG*HQYLNAGLVVALCSTWLKRTGHVGDTHFDQTDALPEQFVKGLTNVSLQGRAQ 252
            VPLQV +PLD + L+    GL G HQY+NAGL VAL +TWL+R GH    H   +  LP+QF++GL++  LQGRAQ
Sbjct:  308 VPLQVVQPLDPQQLDDQPLGLHGEHQYMNAGLAVALVNTWLQRQGHFNILHKKHSVTLPDQFIEGLSSACLQGRAQ 383

[Close]

BLAST of GR914286 vs. TAIR peptide
Match: AT5G05980.2 (| Symbols: ATDFB, DFB, FPGS1 | DHFS-FPGS homolog B | chr5:1799738-1804177 REVERSE LENGTH=513)

HSP 1 Score: 80.8777 bits (198), Expect = 1.818e-16
Identity = 40/77 (51.95%), Postives = 53/77 (68.83%), Query Frame = 1

Query:   22 QVPLQVARPLDAKLLNGLTFGLEG*HQYLNAGLVVALCSTWLKRTGHVGDTHFDQTDALPEQFVKGLTNVSLQGRAQ 252
            +V L+V +PL A+LL+G   GL+G HQY+NAGL V+L S WL++ G +      Q   LPE+F+KGL   SLQGRAQ
Sbjct:  231 EVNLEVVQPLTARLLSGQKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 307

[Close]

BLAST of GR914286 vs. TAIR peptide
Match: AT5G05980.1 (| Symbols: ATDFB, DFB, FPGS1 | DHFS-FPGS homolog B | chr5:1799738-1804441 REVERSE LENGTH=571)

HSP 1 Score: 80.8777 bits (198), Expect = 1.818e-16
Identity = 40/77 (51.95%), Postives = 53/77 (68.83%), Query Frame = 1

Query:   22 QVPLQVARPLDAKLLNGLTFGLEG*HQYLNAGLVVALCSTWLKRTGHVGDTHFDQTDALPEQFVKGLTNVSLQGRAQ 252
            +V L+V +PL A+LL+G   GL+G HQY+NAGL V+L S WL++ G +      Q   LPE+F+KGL   SLQGRAQ
Sbjct:  289 EVNLEVVQPLTARLLSGQKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 365

[Close]

BLAST of GR914286 vs. TAIR peptide
Match: AT3G55630.2 (| Symbols: ATDFD, DFD, FPGS3 | DHFS-FPGS homolog D | chr3:20636785-20639395 FORWARD LENGTH=491)

HSP 1 Score: 77.0258 bits (188), Expect = 2.626e-15
Identity = 41/76 (53.95%), Postives = 49/76 (64.47%), Query Frame = 1

Query:   22 QVPLQVARPLDAKLLNGLTFGLEG*HQYLNAGLVVALCSTWLKRTGHVGDTHFDQTDALPEQFVKGLTNVSLQGRA 249
            +V LQV  PLD+        GL+G HQYLNAGL VALCST+LK  G       DQT+ LPE+F+ GL+N  L GRA
Sbjct:  234 EVNLQVVEPLDSSQ----RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRA 305

[Close]

BLAST of GR914286 vs. TAIR peptide
Match: AT3G55630.3 (| Symbols: ATDFD, DFD, FPGS3 | DHFS-FPGS homolog D | chr3:20636785-20639395 FORWARD LENGTH=492)

HSP 1 Score: 77.0258 bits (188), Expect = 2.626e-15
Identity = 41/76 (53.95%), Postives = 49/76 (64.47%), Query Frame = 1

Query:   22 QVPLQVARPLDAKLLNGLTFGLEG*HQYLNAGLVVALCSTWLKRTGHVGDTHFDQTDALPEQFVKGLTNVSLQGRA 249
            +V LQV  PLD+        GL+G HQYLNAGL VALCST+LK  G       DQT+ LPE+F+ GL+N  L GRA
Sbjct:  235 EVNLQVVEPLDSSQ----RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRA 306

[Close]

BLAST of GR914286 vs. TAIR peptide
Match: AT3G55630.1 (| Symbols: ATDFD, DFD, FPGS3 | DHFS-FPGS homolog D | chr3:20636785-20639395 FORWARD LENGTH=470)

HSP 1 Score: 77.0258 bits (188), Expect = 2.626e-15
Identity = 41/76 (53.95%), Postives = 49/76 (64.47%), Query Frame = 1

Query:   22 QVPLQVARPLDAKLLNGLTFGLEG*HQYLNAGLVVALCSTWLKRTGHVGDTHFDQTDALPEQFVKGLTNVSLQGRA 249
            +V LQV  PLD+        GL+G HQYLNAGL VALCST+LK  G       DQT+ LPE+F+ GL+N  L GRA
Sbjct:  213 EVNLQVVEPLDSSQ----RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRA 284

[Close]

BLAST of GR914286 vs. TAIR peptide
Match: AT3G10160.1 (| Symbols: ATDFC, DFC, FPGS2 | DHFS-FPGS homolog C | chr3:3139588-3143949 REVERSE LENGTH=625)

HSP 1 Score: 67.3958 bits (163), Expect = 2.081e-12
Identity = 36/80 (45.00%), Postives = 50/80 (62.50%), Query Frame = 1

Query:   22 QVPLQVARPLDAKLLNGLTFGLEG*HQYLNAGLVVALCSTWLKRTGH---VGDTHFDQTDALPEQFVKGLTNVSLQGRAQ 252
            +VPL+V  PL+ K L+G+T GL G HQ +NAGL V+L   WL+RTG+   +      +T+ +P  F +GL    L GRAQ
Sbjct:  308 EVPLEVVAPLEPKKLDGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETE-IPVAFCRGLATARLHGRAQ 386

The following BLAST results are available for this feature:

BLAST of GR914286 vs. TrEMBL
BLAST of GR914286 vs. TAIR peptide

BLAST of GR914286 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7TLM3_VITVI	1.111e-23	69.74	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9HMM6_POPTR	2.096e-22	71.05	Folylpolyglutamate synthase OS=Populus trichocarpa...	[more]
B9SE17_RICCO	5.162e-21	65.79	Folylpolyglutamate synthase, putative OS=Ricinus c...	[more]
B9HT54_POPTR	5.162e-21	69.74	Folylpolyglutamate synthase OS=Populus trichocarpa...	[more]
Q10SU1_ORYSJ	1.718e-16	57.89	FolC bifunctional protein, expressed OS=Oryza sati...	[more]
B9FAE3_ORYSJ	1.718e-16	57.89	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B8ALG2_ORYSI	1.718e-16	57.89	Putative uncharacterized protein OS=Oryza sativa s...	[more]
C5WWE5_SORBI	6.530e-16	53.95	Putative uncharacterized protein Sb01g017810 OS=So...	[more]
B7F7H0_ORYSJ	1.114e-15	53.25	cDNA clone:J033039A16, full insert sequence OS=Ory...	[more]
Q337F3_ORYSJ	1.455e-15	53.95	Dihydrofolate synthetase/folylpolyglutamate synthe...	[more]

BLAST of GR914286 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT5G05980.2	1.818e-16	51.95	\| Symbols: ATDFB, DFB, FPGS1 \| DHFS-FPGS homolog B...	[more]
AT5G05980.1	1.818e-16	51.95	\| Symbols: ATDFB, DFB, FPGS1 \| DHFS-FPGS homolog B...	[more]
AT3G55630.2	2.626e-15	53.95	\| Symbols: ATDFD, DFD, FPGS3 \| DHFS-FPGS homolog D...	[more]
AT3G55630.3	2.626e-15	53.95	\| Symbols: ATDFD, DFD, FPGS3 \| DHFS-FPGS homolog D...	[more]
AT3G55630.1	2.626e-15	53.95	\| Symbols: ATDFD, DFD, FPGS3 \| DHFS-FPGS homolog D...	[more]
AT3G10160.1	2.081e-12	45.00	\| Symbols: ATDFC, DFC, FPGS2 \| DHFS-FPGS homolog C...	[more]

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR914286 ID=GR914286; Name=GR914286; organism=Cicer arietinum; type=EST; length=253bp
GATTTCGAGCGGCCGCCCGGGCAGGTACCTCTTCAAGTGGCACGCCCGTT
AGACGCCAAATTGCTAAATGGTTTAACATTTGGTCTTGAAGGCTAACATC
AATATTTAAATGCCGGTCTTGTAGTTGCACTGTGCTCGACATGGTTGAAA
AGGACCGGACATGTTGGAGACACACACTTCGATCAAACCGACGCTTTGCC
TGAACAGTTTGTAAAAGGGTTAACAAATGTTAGTTTGCAAGGAAGGGCTC
AGA

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