GT621244

EST Overview


Name	GT621244
Unique Name	GT621244
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	581

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
KEGG analysis for Lens culinaris unigene v1	2011-01-05
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT621244 vs. TrEMBL
Match: B7FGH5_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=4 SV=1)

HSP 1 Score: 216.853 bits (551), Expect = 8.394e-55
Identity = 106/108 (98.15%), Postives = 108/108 (100.00%), Query Frame = 3

Query:    3 RFVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEMLKFSKDY 326
            RFVVPEGTKKV+YERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAE+LKFSKDY
Sbjct:    9 RFVVPEGTKKVAYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAELLKFSKDY 116

[Close]

BLAST of GT621244 vs. TrEMBL
Match: C6SW39_SOYBN (Putative uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 208.379 bits (529), Expect = 2.986e-52
Identity = 100/108 (92.59%), Postives = 106/108 (98.15%), Query Frame = 3

Query:    3 RFVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEMLKFSKDY 326
            RFVVPEGTKKVSYERDTKIINAASFTIERE+HTIGNILRMQLH+DPNVLFAGYKLPHPLQYKII+RIHTTSQSSPMQAYNQS+NDLD+ELD LKS FEAEMLKFS+DY
Sbjct:    9 RFVVPEGTKKVSYERDTKIINAASFTIEREEHTIGNILRMQLHRDPNVLFAGYKLPHPLQYKIIVRIHTTSQSSPMQAYNQSVNDLDRELDHLKSAFEAEMLKFSRDY 116

[Close]

BLAST of GT621244 vs. TrEMBL
Match: D7SV69_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_8.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00036004001 PE=4 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 6.883e-49
Identity = 95/108 (87.96%), Postives = 102/108 (94.44%), Query Frame = 3

Query:    3 RFVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEMLKFSKDY 326
            RFVVPEGTKKVSYERDTKIINAASFT+EREDHTIGNILRMQLH+D NVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQ+INDLDKELD LK+ FE E+ K S++Y
Sbjct:    9 RFVVPEGTKKVSYERDTKIINAASFTVEREDHTIGNILRMQLHRDENVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQAINDLDKELDYLKNAFEVELAKHSREY 116

[Close]

BLAST of GT621244 vs. TrEMBL
Match: D7LU23_ARALY (ATRPB13.6 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_906569 PE=4 SV=1)

HSP 1 Score: 191.815 bits (486), Expect = 2.892e-47
Identity = 92/108 (85.19%), Postives = 102/108 (94.44%), Query Frame = 3

Query:    3 RFVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEMLKFSKDY 326
            RFVVPEGTKKVSY+RDTKIINAASFTIEREDHTIGNI+RMQLH+D NVLFAGY+LPHPL+YKII+RIHTTSQSSPMQAYNQ+INDLDKELD LK+ FEAE+ KFS  +
Sbjct:    9 RFVVPEGTKKVSYDRDTKIINAASFTIEREDHTIGNIVRMQLHRDENVLFAGYQLPHPLKYKIIVRIHTTSQSSPMQAYNQAINDLDKELDYLKNQFEAEVAKFSNQF 116

[Close]

BLAST of GT621244 vs. TrEMBL
Match: A9PCB4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_722670 PE=4 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 1.099e-46
Identity = 91/101 (90.10%), Postives = 98/101 (97.03%), Query Frame = 3

Query:    3 RFVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEM 305
            RFVVPEGTKKVSYERDTKIINAASFT+EREDHTIGNILRMQLH+D NVLFAGYKLPHPL+YKII+RIHTTSQSSPMQAYNQ+INDLDKELD LK+ FEAE+
Sbjct:    9 RFVVPEGTKKVSYERDTKIINAASFTVEREDHTIGNILRMQLHRDDNVLFAGYKLPHPLKYKIIVRIHTTSQSSPMQAYNQAINDLDKELDHLKNAFEAEL 109

[Close]

BLAST of GT621244 vs. TrEMBL
Match: A5BQX0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_015574 PE=4 SV=1)

HSP 1 Score: 187.963 bits (476), Expect = 4.176e-46
Identity = 95/121 (78.51%), Postives = 102/121 (84.30%), Query Frame = 3

Query:    3 RFVVPEGTKK-------------VSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEMLKFSKDY 326
            RFVVPEGTKK             VSYERDTKIINAASFT+EREDHTIGNILRMQLH+D NVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQ+INDLDKELD LK+ FE E+ K S++Y
Sbjct:    9 RFVVPEGTKKYIVFSLLSQKLSLVSYERDTKIINAASFTVEREDHTIGNILRMQLHRDENVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQAINDLDKELDYLKNAFEVELAKHSREY 129

[Close]

BLAST of GT621244 vs. TrEMBL
Match: B9RVP3_RICCO (DNA-directed RNA polymerase II 13.6 kD polypeptide, putative OS=Ricinus communis GN=RCOM_0968040 PE=4 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 7.123e-46
Identity = 90/98 (91.84%), Postives = 95/98 (96.94%), Query Frame = 3

Query:    3 RFVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFE 296
            RFVVPEGTKKVSYERDTKIINAASFT+EREDHTIGNILRMQLH+D NVLFAGY+LPHPLQYKII+RIHTTSQSSPMQAYNQ+INDLDKELD LKS FE
Sbjct:    9 RFVVPEGTKKVSYERDTKIINAASFTVEREDHTIGNILRMQLHRDENVLFAGYRLPHPLQYKIIVRIHTTSQSSPMQAYNQAINDLDKELDHLKSAFE 106

[Close]

BLAST of GT621244 vs. TrEMBL
Match: C5YC44_SORBI (Putative uncharacterized protein Sb06g001360 OS=Sorghum bicolor GN=Sb06g001360 PE=4 SV=1)

HSP 1 Score: 185.267 bits (469), Expect = 2.707e-45
Identity = 88/104 (84.62%), Postives = 97/104 (93.27%), Query Frame = 3

Query:    3 RFVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEMLKF 314
            RFVVPEGTKKVSYERDTKI+NAASFTIEREDHTIGNI+RMQLH+DPNVLFAGYKLPHPLQYKII+RIHTTSQSSP QAY+Q++NDLDKEL+ LK  FE E  +F
Sbjct:    9 RFVVPEGTKKVSYERDTKIVNAASFTIEREDHTIGNIVRMQLHRDPNVLFAGYKLPHPLQYKIIVRIHTTSQSSPTQAYSQAVNDLDKELEYLKQAFEDEKNRF 112

[Close]

BLAST of GT621244 vs. TrEMBL
Match: B4FJS1_MAIZE (DNA-directed RNA polymerase II subunit J OS=Zea mays PE=4 SV=1)

HSP 1 Score: 184.496 bits (467), Expect = 4.617e-45
Identity = 87/104 (83.65%), Postives = 96/104 (92.31%), Query Frame = 3

Query:    3 RFVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEMLKF 314
            RFVVPEGTKKVSYERDTKI+NAASFT+EREDHT+GNILRMQLH+DPNVLFAGYKLPHPLQYKII+RIHTTSQSSP QAY Q+INDLDKEL+ LK  FE E  ++
Sbjct:    9 RFVVPEGTKKVSYERDTKIVNAASFTVEREDHTVGNILRMQLHRDPNVLFAGYKLPHPLQYKIIVRIHTTSQSSPTQAYTQAINDLDKELEYLKQAFEDEKNRY 112

[Close]

BLAST of GT621244 vs. TrEMBL
Match: C5X6I3_SORBI (Putative uncharacterized protein Sb02g000210 OS=Sorghum bicolor GN=Sb02g000210 PE=4 SV=1)

HSP 1 Score: 182.57 bits (462), Expect = 1.754e-44
Identity = 85/106 (80.19%), Postives = 97/106 (91.51%), Query Frame = 3

Query:    3 RFVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEMLKFSK 320
            RFVVPEGTKKVSYERDTKI+NAASFT+EREDHT+GNILRMQLH+DPNVLFAGYKLPHPLQYKII+RIHT SQSSP QAY+Q++NDLDKEL+ LK  FE E  ++ +
Sbjct:    9 RFVVPEGTKKVSYERDTKIVNAASFTVEREDHTVGNILRMQLHRDPNVLFAGYKLPHPLQYKIIVRIHTMSQSSPTQAYSQAVNDLDKELEYLKQAFEDEKNRYEE 114

[Close]

BLAST of GT621244 vs. SwissProt
Match: RPB11_ARATH (DNA-directed RNA polymerase II subunit RPB11 OS=Arabidopsis thaliana GN=RPB13.6 PE=2 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 2.707e-48
Identity = 91/108 (84.26%), Postives = 102/108 (94.44%), Query Frame = 3

Query:    3 RFVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEMLKFSKDY 326
            RFVVPEGTKKVSY+RDTKIINAASFT+EREDHTIGNI+RMQLH+D NVLFAGY+LPHPL+YKII+RIHTTSQSSPMQAYNQ+INDLDKELD LK+ FEAE+ KFS  +
Sbjct:    9 RFVVPEGTKKVSYDRDTKIINAASFTVEREDHTIGNIVRMQLHRDENVLFAGYQLPHPLKYKIIVRIHTTSQSSPMQAYNQAINDLDKELDYLKNQFEAEVAKFSNQF 116

[Close]

BLAST of GT621244 vs. SwissProt
Match: RPB11_MOUSE (DNA-directed RNA polymerase II subunit RPB11 OS=Mus musculus GN=Polr2j PE=2 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 2.465e-25
Identity = 53/96 (55.21%), Postives = 71/96 (73.96%), Query Frame = 3

Query:    6 FVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGF 293
            F++ EG KK++  +DTK+ NA  FTI +EDHT+GNI++ QL KDP VLFAGYK+PHPL++KIIIR+ TT   SP +A+  +I DL  EL  L+  F
Sbjct:   10 FLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERF 105

[Close]

BLAST of GT621244 vs. SwissProt
Match: RPB11_HUMAN (DNA-directed RNA polymerase II subunit RPB11-a OS=Homo sapiens GN=POLR2J PE=1 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 2.465e-25
Identity = 53/96 (55.21%), Postives = 71/96 (73.96%), Query Frame = 3

Query:    6 FVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGF 293
            F++ EG KK++  +DTK+ NA  FTI +EDHT+GNI++ QL KDP VLFAGYK+PHPL++KIIIR+ TT   SP +A+  +I DL  EL  L+  F
Sbjct:   10 FLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERF 105

[Close]

BLAST of GT621244 vs. SwissProt
Match: RPB11_DROME (DNA-directed RNA polymerase II subunit RPB11 OS=Drosophila melanogaster GN=Rpb11 PE=1 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 2.465e-25
Identity = 54/97 (55.67%), Postives = 72/97 (74.23%), Query Frame = 3

Query:    6 FVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFE 296
            F++ EG KK+  E DTK+ NAA FTI +EDHT+GN++R QL KDPNVLFAGYK+PHPL++K +IRI TT+  SP +A+  +I DL  EL   +  F+
Sbjct:   10 FLLYEGEKKIIKELDTKVTNAAIFTINKEDHTLGNMIRNQLLKDPNVLFAGYKVPHPLEHKFVIRIQTTADYSPQEAFMNAITDLLAELSLFEERFK 106

[Close]

BLAST of GT621244 vs. SwissProt
Match: RPB11_BOVIN (DNA-directed RNA polymerase II subunit RPB11 OS=Bos taurus GN=POLR2J PE=3 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 2.465e-25
Identity = 53/96 (55.21%), Postives = 71/96 (73.96%), Query Frame = 3

Query:    6 FVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGF 293
            F++ EG KK++  +DTK+ NA  FTI +EDHT+GNI++ QL KDP VLFAGYK+PHPL++KIIIR+ TT   SP +A+  +I DL  EL  L+  F
Sbjct:   10 FLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERF 105

[Close]

BLAST of GT621244 vs. SwissProt
Match: RPB11_ANOGA (DNA-directed RNA polymerase II subunit RPB11 OS=Anopheles gambiae GN=Rpb11 PE=3 SV=2)

HSP 1 Score: 114.39 bits (285), Expect = 4.205e-25
Identity = 54/100 (54.00%), Postives = 72/100 (72.00%), Query Frame = 3

Query:    6 FVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEM 305
            F++ EG KK+  E D K+ NAA FT+ +EDHT+GN++R QL KDPNVLFAGYKLPHPL++K ++RI TTS+ SP +A+  +I DL  EL   +  F   M
Sbjct:   10 FLLYEGEKKIIKELDMKVPNAAIFTVNKEDHTLGNMIRNQLLKDPNVLFAGYKLPHPLEHKFVLRIQTTSEYSPHEAFMNAITDLLSELSLFEERFREAM 109

[Close]

BLAST of GT621244 vs. SwissProt
Match: RPB1B_HUMAN (DNA-directed RNA polymerase II subunit RPB11-b1 OS=Homo sapiens GN=POLR2J2 PE=1 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 7.931e-24
Identity = 53/101 (52.48%), Postives = 73/101 (72.28%), Query Frame = 3

Query:    6 FVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEML 308
            F++ EG +K++  +DTK+ NA  FTI +EDHT+GNI++ QL KDP VLFAGYK+PHPL++KIIIR+ TT   SP +A+  +I DL  EL  L+  F   +L
Sbjct:   10 FLLFEG-EKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRTCLL 109

[Close]

BLAST of GT621244 vs. SwissProt
Match: RPB1C_HUMAN (DNA-directed RNA polymerase II subunit RPB11-b2 OS=Homo sapiens GN=POLR2J3 PE=3 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 1.767e-23
Identity = 52/101 (51.49%), Postives = 73/101 (72.28%), Query Frame = 3

Query:    6 FVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEML 308
            F++ EG +K++  +DTK+ NA  FT+ +EDHT+GNI++ QL KDP VLFAGYK+PHPL++KIIIR+ TT   SP +A+  +I DL  EL  L+  F   +L
Sbjct:   10 FLLFEG-EKITINKDTKVPNACLFTMNKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRTCLL 109

[Close]

BLAST of GT621244 vs. SwissProt
Match: RPB11_DICDI (DNA-directed RNA polymerase II subunit rpb11 OS=Dictyostelium discoideum GN=polr2j PE=3 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 2.308e-23
Identity = 48/102 (47.06%), Postives = 76/102 (74.51%), Query Frame = 3

Query:    6 FVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEMLK 311
            FV+P+G KKV+  RDTKI NA+ FTI +EDHTIGN++RMQL  D +++FAGY++PHPL++ + IRI T + ++P++   +S+  L +E  +L++ F  ++ K
Sbjct:   10 FVLPDGAKKVTMVRDTKIPNASMFTILKEDHTIGNLIRMQLIADQDIIFAGYRMPHPLEHNVNIRIQTNNNTNPLECVQKSMECLSREFTSLENSFIEQVQK 111

[Close]

BLAST of GT621244 vs. SwissProt
Match: RPB11_CAEBR (Probable DNA-directed RNA polymerase II subunit RPB11 OS=Caenorhabditis briggsae GN=CBG20834 PE=3 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 1.953e-22
Identity = 49/95 (51.58%), Postives = 70/95 (73.68%), Query Frame = 3

Query:   27 KKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEMLK 311
            KK+  E+DTK+ NAA FTI +EDHT+GN+L++QL KDP VLFAGYK PHPL++KI++R+ TT+ ++P  A   +I DL  EL  L+   ++ + K
Sbjct:   16 KKIYIEKDTKVPNAAIFTINKEDHTLGNMLKIQLLKDPEVLFAGYKNPHPLEHKILLRVQTTNATTPADALTTAITDLVGELSLLEHRIDSAIKK 110

[Close]

BLAST of GT621244 vs. TAIR peptide
Match: AT3G52090.1 (| Symbols: ATRPB13.6, NRPB11, NRPD11, NRPE11 | DNA-directed RNA polymerase, RBP11-like | chr3:19318069-19318998 REVERSE LENGTH=116)

HSP 1 Score: 191.43 bits (485), Expect = 2.864e-49
Identity = 91/108 (84.26%), Postives = 102/108 (94.44%), Query Frame = 3

Query:    3 RFVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEMLKFSKDY 326
            RFVVPEGTKKVSY+RDTKIINAASFT+EREDHTIGNI+RMQLH+D NVLFAGY+LPHPL+YKII+RIHTTSQSSPMQAYNQ+INDLDKELD LK+ FEAE+ KFS  +
Sbjct:    9 RFVVPEGTKKVSYDRDTKIINAASFTVEREDHTIGNIVRMQLHRDENVLFAGYQLPHPLKYKIIVRIHTTSQSSPMQAYNQAINDLDKELDYLKNQFEAEVAKFSNQF 116

[Close]

BLAST of GT621244 vs. TAIR peptide
Match: AT3G52090.2 (| Symbols: NRPB11 | DNA-directed RNA polymerase, RBP11-like | chr3:19318069-19318998 REVERSE LENGTH=146)

HSP 1 Score: 175.637 bits (444), Expect = 1.626e-44
Identity = 91/138 (65.94%), Postives = 102/138 (73.91%), Query Frame = 3

Query:    3 RFVVPEGTKKVSYERDTKIINAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIR------------------------------IHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEMLKFSKDY 326
            RFVVPEGTKKVSY+RDTKIINAASFT+EREDHTIGNI+RMQLH+D NVLFAGY+LPHPL+YKII+R                              IHTTSQSSPMQAYNQ+INDLDKELD LK+ FEAE+ KFS  +
Sbjct:    9 RFVVPEGTKKVSYDRDTKIINAASFTVEREDHTIGNIVRMQLHRDENVLFAGYQLPHPLKYKIIVRDPKDWFRNLKVVYGCLETVNISWFKLLVLTIHTTSQSSPMQAYNQAINDLDKELDYLKNQFEAEVAKFSNQF 146

[Close]

BLAST of GT621244 vs. TAIR peptide
Match: AT2G29540.2 (| Symbols: ATRPC14, ATRPAC14, RPC14 | RNApolymerase 14 kDa subunit | chr2:12642727-12643828 FORWARD LENGTH=122)

HSP 1 Score: 55.4546 bits (132), Expect = 2.453e-8
Identity = 26/88 (29.55%), Postives = 50/88 (56.82%), Query Frame = 3

Query:   60 INAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEMLKFSKD 323
            ++ ASFT+  EDHT+ N +R  L++DP V  A Y +PHP   ++ IR+ TT   +  + +  +  +L +    ++S F+  + ++  +
Sbjct:    9 VSHASFTLSEEDHTLANAVRFVLNQDPRVTVAAYTIPHPSLEQVNIRVQTTGDPA-REVFKDACQELMQMNRHVRSVFDKAVAEYKDE 95

[Close]

BLAST of GT621244 vs. TAIR peptide
Match: AT2G29540.1 (| Symbols: ATRPC14, ATRPAC14, RPC14 | RNApolymerase 14 kDa subunit | chr2:12642727-12643828 FORWARD LENGTH=122)

HSP 1 Score: 55.4546 bits (132), Expect = 2.453e-8
Identity = 26/88 (29.55%), Postives = 50/88 (56.82%), Query Frame = 3

Query:   60 INAASFTIEREDHTIGNILRMQLHKDPNVLFAGYKLPHPLQYKIIIRIHTTSQSSPMQAYNQSINDLDKELDTLKSGFEAEMLKFSKD 323
            ++ ASFT+  EDHT+ N +R  L++DP V  A Y +PHP   ++ IR+ TT   +  + +  +  +L +    ++S F+  + ++  +
Sbjct:    9 VSHASFTLSEEDHTLANAVRFVLNQDPRVTVAAYTIPHPSLEQVNIRVQTTGDPA-REVFKDACQELMQMNRHVRSVFDKAVAEYKDE 95

The following BLAST results are available for this feature:

BLAST of GT621244 vs. TrEMBL
BLAST of GT621244 vs. SwissProt
BLAST of GT621244 vs. TAIR peptide

BLAST of GT621244 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FGH5_MEDTR	8.394e-55	98.15	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6SW39_SOYBN	2.986e-52	92.59	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7SV69_VITVI	6.883e-49	87.96	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7LU23_ARALY	2.892e-47	85.19	ATRPB13.6 OS=Arabidopsis lyrata subsp. lyrata GN=A...	[more]
A9PCB4_POPTR	1.099e-46	90.10	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A5BQX0_VITVI	4.176e-46	78.51	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9RVP3_RICCO	7.123e-46	91.84	DNA-directed RNA polymerase II 13.6 kD polypeptide...	[more]
C5YC44_SORBI	2.707e-45	84.62	Putative uncharacterized protein Sb06g001360 OS=So...	[more]
B4FJS1_MAIZE	4.617e-45	83.65	DNA-directed RNA polymerase II subunit J OS=Zea ma...	[more]
C5X6I3_SORBI	1.754e-44	80.19	Putative uncharacterized protein Sb02g000210 OS=So...	[more]

BLAST of GT621244 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
RPB11_ARATH	2.707e-48	84.26	DNA-directed RNA polymerase II subunit RPB11 OS=Ar...	[more]
RPB11_MOUSE	2.465e-25	55.21	DNA-directed RNA polymerase II subunit RPB11 OS=Mu...	[more]
RPB11_HUMAN	2.465e-25	55.21	DNA-directed RNA polymerase II subunit RPB11-a OS=...	[more]
RPB11_DROME	2.465e-25	55.67	DNA-directed RNA polymerase II subunit RPB11 OS=Dr...	[more]
RPB11_BOVIN	2.465e-25	55.21	DNA-directed RNA polymerase II subunit RPB11 OS=Bo...	[more]
RPB11_ANOGA	4.205e-25	54.00	DNA-directed RNA polymerase II subunit RPB11 OS=An...	[more]
RPB1B_HUMAN	7.931e-24	52.48	DNA-directed RNA polymerase II subunit RPB11-b1 OS...	[more]
RPB1C_HUMAN	1.767e-23	51.49	DNA-directed RNA polymerase II subunit RPB11-b2 OS...	[more]
RPB11_DICDI	2.308e-23	47.06	DNA-directed RNA polymerase II subunit rpb11 OS=Di...	[more]
RPB11_CAEBR	1.953e-22	51.58	Probable DNA-directed RNA polymerase II subunit RP...	[more]

BLAST of GT621244 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT3G52090.1	2.864e-49	84.26	\| Symbols: ATRPB13.6, NRPB11, NRPD11, NRPE11 \| DNA...	[more]
AT3G52090.2	1.626e-44	65.94	\| Symbols: NRPB11 \| DNA-directed RNA polymerase, R...	[more]
AT2G29540.2	2.453e-8	29.55	\| Symbols: ATRPC14, ATRPAC14, RPC14 \| RNApolymeras...	[more]
AT2G29540.1	2.453e-8	29.55	\| Symbols: ATRPC14, ATRPAC14, RPC14 \| RNApolymeras...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008193	DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved site	PROSITE	PS01154	RNA_POL_L_13KD	coord: 27..58 score
IPR009025	DNA-directed RNA polymerase, RBP11-like	SUPERFAMILY	55257	RBP11-like subunits of RNA polymerase	coord: 1..106 score: 4.1
IPR011261	DNA-directed RNA polymerase, dimerisation	PFAM	PF01193	RNA_pol_L	coord: 24..94 score: 3.3
None	No IPR available	GENE3D	3.30.1360.10	no description	coord: 2..105 score: 2.4
None	No IPR available	PANTHER	PTHR13946	DNA-DIRECTED RNA POLYMERASE I,II,III	coord: 1..103 score: 5.3
None	No IPR available	PANTHER	PTHR13946:SF16	DNA-DIRECTED RNA POLYMERASE II 13.3 KDA POLYPEPTIDE	coord: 1..103 score: 5.3

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT621244 ID=GT621244; Name=GT621244; organism=Lens culinaris; type=EST; length=581bp
AGCGTTTCGTCGTTCCTGAAGGCACCAAAAAGGTTTCTTACGAGAGAGAC
ACCAAGATCATCAACGCTGCATCTTTCACCATTGAAAGAGAAGATCACAC
TATCGGAAACATCCTCCGCATGCAACTCCACAAAGACCCCAACGTGCTAT
TCGCTGGATACAAGCTTCCTCATCCTCTTCAGTACAAGATTATTATTAGG
ATACACACCACCAGCCAGTCTTCACCAATGCAGGCATACAACCAGTCTAT
TAACGATCTTGATAAGGAACTTGATACCTTGAAGAGTGGTTTTGAGGCTG
AGATGTTGAAGTTTTCAAAGGACTATTGAGTTACAACAGTCAAGATGGGG
AGTTGTGACCTTTTGCAGATATTGTGACAGCAGACCAGTCCAGTAAAATC
AAATTAAAATAGGATATTTTGTACGGAATTCAAAAGTTATATTAACAAAA
AATCTTTAGAAGGAGGATTTTCACTGGTATTAAAGCAATTAACTACTAGT
TTATTTCTGTTCTGTCACATGGATCTTGTGAATCATGGATTTCATGTCTA
AATATTAGTTTTGAAAGGAATTTTCCAAGAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0003899	DNA-directed 5'-3' RNA polymerase activity
GO:0046983	protein dimerization activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006350	transcription

Vocabulary: **INTERPRO**
Term	Definition
IPR008193	RNA_pol_Rpb11_13-16kDa_CS
IPR009025	RBP11-like_dimer
IPR011261	DNA-directed RNA polymerase, dimerisation

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