GT620104

EST Overview


Name	GT620104
Unique Name	GT620104
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	347

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
KEGG analysis for Lens culinaris unigene v1	2011-01-05
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT620104 vs. TrEMBL
Match: Q3LI84_PEA (Allene oxide cyclase OS=Pisum sativum GN=aoc PE=2 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 3.644e-19
Identity = 48/68 (70.59%), Postives = 51/68 (75.00%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGIPDLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFTN 206
            GIFEGVHG+VKLQQIVFPFKLFYTFYLKGIPDLPD+LLG               KAT+ HASL NFTN
Sbjct:  185 GIFEGVHGKVKLQQIVFPFKLFYTFYLKGIPDLPDELLGNPVEPSPNVEPSPAAKATQPHASLSNFTN 252

[Close]

BLAST of GT620104 vs. TrEMBL
Match: Q711Q9_MEDTR (Allene oxide cyclase OS=Medicago truncatula GN=aoc PE=2 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 8.975e-18
Identity = 46/68 (67.65%), Postives = 49/68 (72.06%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGIPDLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFTN 206
            GIFEGV+GQVKLQQ+VFPFKLFYTFYLKG+ DLP  LLG               KATE HASLPNFTN
Sbjct:  185 GIFEGVYGQVKLQQLVFPFKLFYTFYLKGVADLPADLLGKPVDPSPHVEPSTAAKATEPHASLPNFTN 252

[Close]

BLAST of GT620104 vs. TrEMBL
Match: C6TCY8_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.411e-17
Identity = 44/68 (64.71%), Postives = 48/68 (70.59%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGIPDLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFTN 206
            GIFEGV+GQVKL Q+VFPFKLFYTFYLKG+PDLP +LLG                ATE HA LPNFTN
Sbjct:  188 GIFEGVYGQVKLHQLVFPFKLFYTFYLKGVPDLPPELLGKPVEPSPSVEPSPAAMATEPHACLPNFTN 255

[Close]

BLAST of GT620104 vs. TrEMBL
Match: D7T989_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_11.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00011944001 PE=4 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.097e-15
Identity = 43/67 (64.18%), Postives = 47/67 (70.15%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGIPDLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFT 203
            GIFEGV+G+VKLQQIVFPFKLFYTFYLKGIPDLP  L+G               KAT  HAS+ NFT
Sbjct:  187 GIFEGVYGEVKLQQIVFPFKLFYTFYLKGIPDLPHDLMGKPVTPSPTVEPSPAAKATLPHASVKNFT 253

[Close]

BLAST of GT620104 vs. TrEMBL
Match: A5C260_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_032173 PE=4 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.097e-15
Identity = 43/67 (64.18%), Postives = 47/67 (70.15%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGIPDLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFT 203
            GIFEGV+G+VKLQQIVFPFKLFYTFYLKGIPDLP  L+G               KAT  HAS+ NFT
Sbjct:  177 GIFEGVYGEVKLQQIVFPFKLFYTFYLKGIPDLPHDLMGKPVTPSPTVEPSPAAKATLPHASVKNFT 243

[Close]

BLAST of GT620104 vs. TrEMBL
Match: Q8L6H4_ORYSA (Allene oxide cyclase OS=Oryza sativa GN=aoc PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 3.192e-15
Identity = 42/68 (61.76%), Postives = 46/68 (67.65%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGIPDLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFTN 206
            G+FEG +GQVKL QIVFPFK+FYTFYLKGIPDLP +LL                KATE HA L NFTN
Sbjct:  173 GVFEGAYGQVKLNQIVFPFKIFYTFYLKGIPDLPRELLCTPVPPSPTVEPTPAAKATEPHACLNNFTN 240

[Close]

BLAST of GT620104 vs. TrEMBL
Match: Q75KD7_ORYSJ (AOC OS=Oryza sativa subsp. japonica GN=OSJNBa0054H04.12 PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 3.192e-15
Identity = 42/68 (61.76%), Postives = 46/68 (67.65%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGIPDLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFTN 206
            G+FEG +GQVKL QIVFPFK+FYTFYLKGIPDLP +LL                KATE HA L NFTN
Sbjct:  173 GVFEGAYGQVKLNQIVFPFKIFYTFYLKGIPDLPRELLCTPVPPSPTVEPTPAAKATEPHACLNNFTN 240

[Close]

BLAST of GT620104 vs. TrEMBL
Match: Q711R0_HORVU (Allene oxide cyclase OS=Hordeum vulgare GN=aoc PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 3.192e-15
Identity = 42/68 (61.76%), Postives = 47/68 (69.12%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGIPDLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFTN 206
            G+FEGV+GQVKL QIVFPFK+FYTFYLKGIPDLP +LL                KATE HA L NFT+
Sbjct:  171 GVFEGVYGQVKLNQIVFPFKIFYTFYLKGIPDLPKELLCTPVPPSPTVEPTPAAKATEPHACLNNFTD 238

[Close]

BLAST of GT620104 vs. TrEMBL
Match: A3AJG2_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_11399 PE=4 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 3.192e-15
Identity = 42/68 (61.76%), Postives = 46/68 (67.65%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGIPDLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFTN 206
            G+FEG +GQVKL QIVFPFK+FYTFYLKGIPDLP +LL                KATE HA L NFTN
Sbjct:  174 GVFEGAYGQVKLNQIVFPFKIFYTFYLKGIPDLPRELLCTPVPPSPTVEPTPAAKATEPHACLNNFTN 241

[Close]

BLAST of GT620104 vs. TrEMBL
Match: A2XID3_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_12194 PE=4 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 3.192e-15
Identity = 42/68 (61.76%), Postives = 46/68 (67.65%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGIPDLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFTN 206
            G+FEG +GQVKL QIVFPFK+FYTFYLKGIPDLP +LL                KATE HA L NFTN
Sbjct:  173 GVFEGAYGQVKLNQIVFPFKIFYTFYLKGIPDLPRELLCTPVPPSPTVEPTPAAKATEPHACLNNFTN 240

[Close]

BLAST of GT620104 vs. SwissProt
Match: AOC4_ARATH (Allene oxide cyclase 4, chloroplastic OS=Arabidopsis thaliana GN=AOC4 PE=2 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 1.965e-11
Identity = 34/69 (49.28%), Postives = 45/69 (65.22%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGI-PDLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFTN 206
            G+FEG +GQVKL+Q+V+P KLFYTFYLKG+  DLP +L G               +AT+  A++ NFTN
Sbjct:  186 GVFEGAYGQVKLRQLVYPTKLFYTFYLKGVAADLPVELTGKHVEPSKEVKPAAEAQATQPGATIANFTN 254

[Close]

BLAST of GT620104 vs. SwissProt
Match: AOC1_ARATH (Allene oxide cyclase 1, chloroplastic OS=Arabidopsis thaliana GN=AOC1 PE=1 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 5.718e-11
Identity = 35/69 (50.72%), Postives = 43/69 (62.32%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGIP-DLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFTN 206
            GIFEG +GQVKLQQ+V+P KLFYTFYLKG+  DLP +L+G               KA +    + NFTN
Sbjct:  186 GIFEGAYGQVKLQQLVYPTKLFYTFYLKGLANDLPLELIGTPVPPSKDVEPAPEAKALKPSGVVSNFTN 254

[Close]

BLAST of GT620104 vs. SwissProt
Match: AOC2_ARATH (Allene oxide cyclase 2, chloroplastic OS=Arabidopsis thaliana GN=AOC2 PE=1 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 7.469e-11
Identity = 35/69 (50.72%), Postives = 42/69 (60.87%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGIP-DLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFTN 206
            GIFEG +GQVKLQQ+V+P KLFYTFYLKG+  DLP +L G               KA E    + N+TN
Sbjct:  185 GIFEGAYGQVKLQQLVYPTKLFYTFYLKGLANDLPLELTGTPVPPSKDIEPAPEAKALEPSGVISNYTN 253

[Close]

BLAST of GT620104 vs. SwissProt
Match: AOC3_ARATH (Allene oxide cyclase 3, chloroplastic OS=Arabidopsis thaliana GN=AOC3 PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 9.754e-11
Identity = 35/69 (50.72%), Postives = 42/69 (60.87%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGIP-DLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFTN 206
            GIFEG +GQVKL+Q+V+P KLFYTFYLKG+  DLP +L G               KA E    + NFTN
Sbjct:  190 GIFEGAYGQVKLRQLVYPTKLFYTFYLKGLANDLPLELTGTAVTPSKDVKPAPEAKAMEPSGVISNFTN 258

[Close]

BLAST of GT620104 vs. TAIR peptide
Match: AT1G13280.1 (| Symbols: AOC4 | allene oxide cyclase 4 | chr1:4547624-4548552 FORWARD LENGTH=254)

HSP 1 Score: 67.781 bits (164), Expect = 1.564e-12
Identity = 34/69 (49.28%), Postives = 45/69 (65.22%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGI-PDLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFTN 206
            G+FEG +GQVKL+Q+V+P KLFYTFYLKG+  DLP +L G               +AT+  A++ NFTN
Sbjct:  186 GVFEGAYGQVKLRQLVYPTKLFYTFYLKGVAADLPVELTGKHVEPSKEVKPAAEAQATQPGATIANFTN 254

[Close]

BLAST of GT620104 vs. TAIR peptide
Match: AT3G25760.1 (| Symbols: AOC1, ERD12 | allene oxide cyclase 1 | chr3:9403972-9405105 FORWARD LENGTH=254)

HSP 1 Score: 66.2402 bits (160), Expect = 4.549e-12
Identity = 35/69 (50.72%), Postives = 43/69 (62.32%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGIP-DLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFTN 206
            GIFEG +GQVKLQQ+V+P KLFYTFYLKG+  DLP +L+G               KA +    + NFTN
Sbjct:  186 GIFEGAYGQVKLQQLVYPTKLFYTFYLKGLANDLPLELIGTPVPPSKDVEPAPEAKALKPSGVVSNFTN 254

[Close]

BLAST of GT620104 vs. TAIR peptide
Match: AT3G25770.1 (| Symbols: AOC2 | allene oxide cyclase 2 | chr3:9406975-9407839 FORWARD LENGTH=253)

HSP 1 Score: 65.855 bits (159), Expect = 5.941e-12
Identity = 35/69 (50.72%), Postives = 42/69 (60.87%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGIP-DLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFTN 206
            GIFEG +GQVKLQQ+V+P KLFYTFYLKG+  DLP +L G               KA E    + N+TN
Sbjct:  185 GIFEGAYGQVKLQQLVYPTKLFYTFYLKGLANDLPLELTGTPVPPSKDIEPAPEAKALEPSGVISNYTN 253

[Close]

BLAST of GT620104 vs. TAIR peptide
Match: AT3G25780.1 (| Symbols: AOC3 | allene oxide cyclase 3 | chr3:9409362-9410409 FORWARD LENGTH=258)

HSP 1 Score: 65.4698 bits (158), Expect = 7.760e-12
Identity = 35/69 (50.72%), Postives = 42/69 (60.87%), Query Frame = 3

Query:    3 GIFEGVHGQVKLQQIVFPFKLFYTFYLKGIP-DLPDQLLGXXXXXXXXXXXXXXXKATEHHASLPNFTN 206
            GIFEG +GQVKL+Q+V+P KLFYTFYLKG+  DLP +L G               KA E    + NFTN
Sbjct:  190 GIFEGAYGQVKLRQLVYPTKLFYTFYLKGLANDLPLELTGTAVTPSKDVKPAPEAKAMEPSGVISNFTN 258

The following BLAST results are available for this feature:

BLAST of GT620104 vs. TrEMBL
BLAST of GT620104 vs. SwissProt
BLAST of GT620104 vs. TAIR peptide

BLAST of GT620104 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q3LI84_PEA	3.644e-19	70.59	Allene oxide cyclase OS=Pisum sativum GN=aoc PE=2 ...	[more]
Q711Q9_MEDTR	8.975e-18	67.65	Allene oxide cyclase OS=Medicago truncatula GN=aoc...	[more]
C6TCY8_SOYBN	3.411e-17	64.71	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7T989_VITVI	1.097e-15	64.18	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5C260_VITVI	1.097e-15	64.18	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q8L6H4_ORYSA	3.192e-15	61.76	Allene oxide cyclase OS=Oryza sativa GN=aoc PE=2 S...	[more]
Q75KD7_ORYSJ	3.192e-15	61.76	AOC OS=Oryza sativa subsp. japonica GN=OSJNBa0054H...	[more]
Q711R0_HORVU	3.192e-15	61.76	Allene oxide cyclase OS=Hordeum vulgare GN=aoc PE=...	[more]
A3AJG2_ORYSJ	3.192e-15	61.76	Putative uncharacterized protein OS=Oryza sativa s...	[more]
A2XID3_ORYSI	3.192e-15	61.76	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of GT620104 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
AOC4_ARATH	1.965e-11	49.28	Allene oxide cyclase 4, chloroplastic OS=Arabidops...	[more]
AOC1_ARATH	5.718e-11	50.72	Allene oxide cyclase 1, chloroplastic OS=Arabidops...	[more]
AOC2_ARATH	7.469e-11	50.72	Allene oxide cyclase 2, chloroplastic OS=Arabidops...	[more]
AOC3_ARATH	9.754e-11	50.72	Allene oxide cyclase 3, chloroplastic OS=Arabidops...	[more]

BLAST of GT620104 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT1G13280.1	1.564e-12	49.28	\| Symbols: AOC4 \| allene oxide cyclase 4 \| chr1:45...	[more]
AT3G25760.1	4.549e-12	50.72	\| Symbols: AOC1, ERD12 \| allene oxide cyclase 1 \| ...	[more]
AT3G25770.1	5.941e-12	50.72	\| Symbols: AOC2 \| allene oxide cyclase 2 \| chr3:94...	[more]
AT3G25780.1	7.760e-12	50.72	\| Symbols: AOC3 \| allene oxide cyclase 3 \| chr3:94...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009410	Allene oxide cyclase	PFAM	PF06351	Allene_ox_cyc	coord: 1..66 score: 7.5
None	No IPR available	SEG	seg	seg	coord: 40..54 score
None	No IPR available	SUPERFAMILY	141493	Allene oxide cyclase-like	coord: 1..68 score: 4.6

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT620104 ID=GT620104; Name=GT620104; organism=Lens culinaris; type=EST; length=347bp
CTGGAATCTTTGAAGGTGTTCACGGACAAGTTAAACTGCAACAAATTGTG
TTCCCATTTAAGCTGTTTTACACATTTTACTTGAAGGGTATTCCTGATTT
GCCAGATCAGTTACTTGGGAACCCTGTTGAACCATCTCCAAATGTTGAAC
CTTCTCCTGCTGCTAAGGCGACTGAGCATCATGCCTCTTTACCAAACTTC
ACCAACTGATCGTTAAGAATTTTTTTTTAGAACCCAAAATAAATTGTACT
TGTTTCCTGTTCTCTGTCATTCGATTTTATTTTTGCCTTGTGGGTGATGT
ATTAGATTAAGCAGCATAATTGCAACTACTTTTGTTTATCTCGACCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0009507	chloroplast

Vocabulary: **Molecular Function**
Term	Definition
GO:0016853	isomerase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR009410	Allene_ox_cyc

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