GT621396

EST Overview


Name	GT621396
Unique Name	GT621396
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	785

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
KEGG analysis for Lens culinaris unigene v1	2011-01-05
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT621396 vs. TrEMBL
Match: Q9FY85_ARATH (Branched-chain alpha keto-acid dehydrogenase E1 alpha subunit-like protein OS=Arabidopsis thaliana GN=T5E8_100 PE=4 SV=1)

HSP 1 Score: 275.404 bits (703), Expect = 3.844e-72
Identity = 142/227 (62.56%), Postives = 160/227 (70.48%), Query Frame = 3

Query:    3 GNVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            G V F P+++F+S S  +R+PCYRVLDDNG  I  +  VQVSE+VAVK+Y+DMV LQ MD IFYEAQRQGR+SFY TA GEEAINIASAAAL+  DVIFPQYRE GVLLWRGFTLQEFANQCF NK D GKGRQMP HYGSNK NY TV++T+A                                        TQ+P AVGAAYSLKMDKKDACA+TYFGDGG+SE
Sbjct:   27 GKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIA----------------------------------------TQLPNAVGAAYSLKMDKKDACAVTYFGDGGTSE 213

[Close]

BLAST of GT621396 vs. TrEMBL
Match: Q84JL2_ARATH (Putative branched-chain alpha keto-acid dehydrogenase E1 alpha subunit OS=Arabidopsis thaliana GN=At5g09300 PE=2 SV=1)

HSP 1 Score: 275.404 bits (703), Expect = 3.844e-72
Identity = 142/227 (62.56%), Postives = 160/227 (70.48%), Query Frame = 3

Query:    3 GNVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            G V F P+++F+S S  +R+PCYRVLDDNG  I  +  VQVSE+VAVK+Y+DMV LQ MD IFYEAQRQGR+SFY TA GEEAINIASAAAL+  DVIFPQYRE GVLLWRGFTLQEFANQCF NK D GKGRQMP HYGSNK NY TV++T+A                                        TQ+P AVGAAYSLKMDKKDACA+TYFGDGG+SE
Sbjct:   85 GKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIA----------------------------------------TQLPNAVGAAYSLKMDKKDACAVTYFGDGGTSE 271

[Close]

BLAST of GT621396 vs. TrEMBL
Match: B4F923_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 275.018 bits (702), Expect = 5.021e-72
Identity = 141/227 (62.11%), Postives = 162/227 (71.37%), Query Frame = 3

Query:    3 GNVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            G V+F+ +M FL  ST +RI CYRVLDD+G TI G+   +VS ++A+KMY++MV LQ MDTIFYEAQRQGRISFY+T+NGEEAINIASAAALSMDD++ PQYRE GVLLWRGFTLQEFANQCF NK D GKGRQMP HYGSN+ NY TV+S +A                                        TQ+P AVGAAYSLKMDKKDACAITYFGDGG+SE
Sbjct:  101 GKVSFVAEMNFLPESTRERINCYRVLDDDGRTISGSRFQEVSRELALKMYSEMVTLQIMDTIFYEAQRQGRISFYLTSNGEEAINIASAAALSMDDIVLPQYREPGVLLWRGFTLQEFANQCFGNKLDYGKGRQMPIHYGSNRLNYFTVSSPIA----------------------------------------TQLPHAVGAAYSLKMDKKDACAITYFGDGGTSE 287

[Close]

BLAST of GT621396 vs. TrEMBL
Match: D7M224_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_350155 PE=4 SV=1)

HSP 1 Score: 274.633 bits (701), Expect = 6.557e-72
Identity = 141/227 (62.11%), Postives = 160/227 (70.48%), Query Frame = 3

Query:    3 GNVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            G V F P+++F+S S  +R+PCYRVLDDNG  I  +  VQVSE+VAVK+Y+DMV LQ MD IFYEAQRQGR+SFY TA GEEAINIASAAAL+  DVIFPQYRE GVLLWRGFTLQEFANQCF NK D GKGRQMP HYGSNK NY TV++T+A                                        TQ+P AVGAAYSLKMD+KDACA+TYFGDGG+SE
Sbjct:   85 GKVAFTPEIRFISESDSERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIA----------------------------------------TQLPNAVGAAYSLKMDRKDACAVTYFGDGGTSE 271

[Close]

BLAST of GT621396 vs. TrEMBL
Match: B9H7L5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_559310 PE=4 SV=1)

HSP 1 Score: 272.322 bits (695), Expect = 3.254e-71
Identity = 136/227 (59.91%), Postives = 162/227 (71.37%), Query Frame = 3

Query:    3 GNVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            G V + P+M+FLS S   R+PCYRVLDDNG+ I+G+   Q+SE+VAVK+Y++MV+LQ MDTIFYEAQRQGRISFY+T+ GEEAINIASAAALS DD+I PQYRE G+LLWRGFT+QEFANQCF NK D GKGRQMP HYGS KHN+VT++S +A                                        TQ+PQAVG AYSLKMDKKD+C +TY GDGG+SE
Sbjct:    8 GKVTYTPEMRFLSESNGKRVPCYRVLDDNGEIIIGSDYEQLSEEVAVKIYSNMVSLQMMDTIFYEAQRQGRISFYLTSTGEEAINIASAAALSADDIILPQYREPGILLWRGFTIQEFANQCFGNKDDYGKGRQMPIHYGSKKHNFVTISSPIA----------------------------------------TQLPQAVGIAYSLKMDKKDSCVVTYTGDGGTSE 194

[Close]

BLAST of GT621396 vs. TrEMBL
Match: D7U4C7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_44.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00026470001 PE=4 SV=1)

HSP 1 Score: 270.781 bits (691), Expect = 9.468e-71
Identity = 144/226 (63.72%), Postives = 160/226 (70.80%), Query Frame = 3

Query:    3 GNVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSS 680
            G V F  +M+F+  S ++R  CYRVLDDNG   + ++ VQVS++VAVKMY  M+ LQ MDTIFYEAQRQGRISFYVT+ GEEAIN+ASAAALS+DDVIFPQYRE GVLLWRGFTLQEFANQCF NK D GKGRQMP HYGSNKHNYVTV+STVA                                        TQIPQAVGAAYSLKMD KDAC +TYFGDGGSS
Sbjct:   94 GKVAFTSEMRFIPESPMERAHCYRVLDDNGQPNM-SNFVQVSKEVAVKMYATMLTLQVMDTIFYEAQRQGRISFYVTSIGEEAINVASAAALSIDDVIFPQYREPGVLLWRGFTLQEFANQCFGNKADYGKGRQMPIHYGSNKHNYVTVSSTVA----------------------------------------TQIPQAVGAAYSLKMDGKDACTVTYFGDGGSS 278

[Close]

BLAST of GT621396 vs. TrEMBL
Match: B9RRD6_RICCO (2-oxoisovalerate dehydrogenase, putative OS=Ricinus communis GN=RCOM_1187670 PE=4 SV=1)

HSP 1 Score: 269.626 bits (688), Expect = 2.109e-70
Identity = 141/227 (62.11%), Postives = 158/227 (69.60%), Query Frame = 3

Query:    3 GNVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            G + F P+M+F+S S  +RIPCYRVLDDNG  I    +V VS  +A KMY DMV LQTMDTIFYEAQRQGRISFYVTA GEEAINIASAAAL++DD++ PQYRE GVLLWRGF+LQEFANQCFSNK DN KGRQMPAHYGS KHNY TVAST+A                                        +Q+P AVGAAYSLKMD +DAC + YFGDGGSSE
Sbjct:   63 GKIAFTPEMRFISESPEERIPCYRVLDDNGQLIEDGDNVGVSRHIARKMYCDMVTLQTMDTIFYEAQRQGRISFYVTAIGEEAINIASAAALTIDDLVVPQYREPGVLLWRGFSLQEFANQCFSNKGDNCKGRQMPAHYGSKKHNYFTVASTIA----------------------------------------SQLPHAVGAAYSLKMDGRDACVVAYFGDGGSSE 249

[Close]

BLAST of GT621396 vs. TrEMBL
Match: Q2QWT8_ORYSJ (2-oxoisovalerate dehydrogenase alpha subunit, mitochondrial, putative, expressed OS=Oryza sativa subsp. japonica GN=Os12g0183100 PE=4 SV=2)

HSP 1 Score: 269.24 bits (687), Expect = 2.755e-70
Identity = 137/227 (60.35%), Postives = 161/227 (70.93%), Query Frame = 3

Query:    3 GNVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            G V+F+ +M FL  S  DRI CYRVLDD+G TI G+   +VS+++A+KMYN+M  LQ MDTIF+EAQRQGRISFY+T++GEEAINIASAAAL++DD++ PQYRE GVLLWRGFTLQEFANQCF NK D GKGRQMP HYGSN+ NY TV+S +A                                        TQ+P AVGAAYSLKMDKKDACAITYFGDGG+SE
Sbjct:   99 GKVSFVAEMNFLPESQRDRINCYRVLDDDGRTISGSRFQEVSKELALKMYNEMATLQVMDTIFFEAQRQGRISFYLTSHGEEAINIASAAALTIDDIVLPQYREPGVLLWRGFTLQEFANQCFGNKLDYGKGRQMPIHYGSNRLNYFTVSSPIA----------------------------------------TQLPHAVGAAYSLKMDKKDACAITYFGDGGTSE 285

[Close]

BLAST of GT621396 vs. TrEMBL
Match: Q2QWT6_ORYSJ (2-oxoisovalerate dehydrogenase alpha subunit, mitochondrial, putative, expressed OS=Oryza sativa subsp. japonica GN=LOC_Os12g08260 PE=4 SV=2)

HSP 1 Score: 269.24 bits (687), Expect = 2.755e-70
Identity = 137/227 (60.35%), Postives = 161/227 (70.93%), Query Frame = 3

Query:    3 GNVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            G V+F+ +M FL  S  DRI CYRVLDD+G TI G+   +VS+++A+KMYN+M  LQ MDTIF+EAQRQGRISFY+T++GEEAINIASAAAL++DD++ PQYRE GVLLWRGFTLQEFANQCF NK D GKGRQMP HYGSN+ NY TV+S +A                                        TQ+P AVGAAYSLKMDKKDACAITYFGDGG+SE
Sbjct:  100 GKVSFVAEMNFLPESQRDRINCYRVLDDDGRTISGSRFQEVSKELALKMYNEMATLQVMDTIFFEAQRQGRISFYLTSHGEEAINIASAAALTIDDIVLPQYREPGVLLWRGFTLQEFANQCFGNKLDYGKGRQMPIHYGSNRLNYFTVSSPIA----------------------------------------TQLPHAVGAAYSLKMDKKDACAITYFGDGGTSE 286

[Close]

BLAST of GT621396 vs. TrEMBL
Match: B9GC70_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_35450 PE=4 SV=1)

HSP 1 Score: 269.24 bits (687), Expect = 2.755e-70
Identity = 137/227 (60.35%), Postives = 161/227 (70.93%), Query Frame = 3

Query:    3 GNVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            G V+F+ +M FL  S  DRI CYRVLDD+G TI G+   +VS+++A+KMYN+M  LQ MDTIF+EAQRQGRISFY+T++GEEAINIASAAAL++DD++ PQYRE GVLLWRGFTLQEFANQCF NK D GKGRQMP HYGSN+ NY TV+S +A                                        TQ+P AVGAAYSLKMDKKDACAITYFGDGG+SE
Sbjct:  125 GKVSFVAEMNFLPESQRDRINCYRVLDDDGRTISGSRFQEVSKELALKMYNEMATLQVMDTIFFEAQRQGRISFYLTSHGEEAINIASAAALTIDDIVLPQYREPGVLLWRGFTLQEFANQCFGNKLDYGKGRQMPIHYGSNRLNYFTVSSPIA----------------------------------------TQLPHAVGAAYSLKMDKKDACAITYFGDGGTSE 311

[Close]

BLAST of GT621396 vs. SwissProt
Match: ODBA_DICDI (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Dictyostelium discoideum GN=bkdA PE=3 SV=1)

HSP 1 Score: 170.244 bits (430), Expect = 1.131e-41
Identity = 88/208 (42.31%), Postives = 117/208 (56.25%), Query Frame = 3

Query:   60 IPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            IPCY ++D  G           S++  +KMY  M+ L  MD+I Y+ QRQGRISFY+T+ GEEAI+I SAAAL M D IF QYRE GV +WRGFT+ +  NQC +N+ D GKGRQMP H+GS K N  T++S +                                        +TQ+PQAVG++Y+ K+  +  C I YFG+G +SE
Sbjct:   64 IPCYTIMDQEGVVSKPDQDPNFSKEEVIKMYTTMLTLNVMDSILYDVQRQGRISFYMTSFGEEAIHIGSAAALEMSDTIFAQYRETGVFMWRGFTINDIINQCCTNEHDLGKGRQMPMHFGSRKINLQTISSPL----------------------------------------TTQLPQAVGSSYAQKLAGEKNCTIVYFGEGAASE 231

[Close]

BLAST of GT621396 vs. SwissProt
Match: ODBA_RAT (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial (Fragment) OS=Rattus norvegicus GN=Bckdha PE=1 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 4.751e-40
Identity = 87/226 (38.50%), Postives = 123/226 (54.42%), Query Frame = 3

Query:    6 NVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            +  F+ +++F+  + +  IP YRV+D  G  I  +    + ++  +K+Y  M  L TMD I YE+QRQGRISFY+T  GEE  ++ SAAAL   D++F QYRE GVL++R + L+ F  QC+ N  D GKGRQMP HYG  + ++VT++S +A                                        TQIPQAVGAAY+ K    +   I YFG+G +SE
Sbjct:   54 SAEFVDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPQEEVLKLYRSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALERTDLVFGQYREAGVLMYRDYPLELFMAQCYGNVSDPGKGRQMPVHYGCKERHFVTISSPLA----------------------------------------TQIPQAVGAAYAAKRANANQIVICYFGEGAASE 239

[Close]

BLAST of GT621396 vs. SwissProt
Match: ODBA_PANTR (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Pan troglodytes GN=BCKDHA PE=2 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 1.058e-39
Identity = 88/226 (38.94%), Postives = 123/226 (54.42%), Query Frame = 3

Query:    6 NVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            +  FI +++F+  + +  IP YRV+D  G  I  +    + ++  +K+Y  M  L TMD I YE+QRQGRISFY+T  GEE  ++ SAAAL   D++F QYRE GVL++R + L+ F  QC+ N  D GKGRQMP HYG  + ++VT++S +A                                        TQIPQAVGAAY+ K    +   I YFG+G +SE
Sbjct:   58 SAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLA----------------------------------------TQIPQAVGAAYAAKRANANRVVICYFGEGAASE 243

[Close]

BLAST of GT621396 vs. SwissProt
Match: ODBA_MOUSE (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Mus musculus GN=Bckdha PE=1 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 1.058e-39
Identity = 86/226 (38.05%), Postives = 123/226 (54.42%), Query Frame = 3

Query:    6 NVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            +  F+ +++F+  + +  IP YRV+D  G  I  +    + ++  +K Y  M  L TMD I YE+QR+GRISFY+T  GEE  ++ SAAAL   D++F QYRE GVL++R + L+ F +QC+ N  D GKGRQMP HYG  + ++VT++S +A                                        TQIPQAVGAAY+ K    +   I YFG+G +SE
Sbjct:   55 SAEFVDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPQEEVLKFYRSMTLLNTMDRILYESQREGRISFYMTNYGEEGTHVGSAAALERTDLVFGQYREAGVLMYRDYPLELFMSQCYGNVNDPGKGRQMPVHYGCKERHFVTISSPLA----------------------------------------TQIPQAVGAAYAAKRANANRIVICYFGEGAASE 240

[Close]

BLAST of GT621396 vs. SwissProt
Match: ODBA_MACFA (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Macaca fascicularis GN=BCKDHA PE=2 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 1.058e-39
Identity = 88/226 (38.94%), Postives = 123/226 (54.42%), Query Frame = 3

Query:    6 NVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            +  FI +++F+  + +  IP YRV+D  G  I  +    + ++  +K+Y  M  L TMD I YE+QRQGRISFY+T  GEE  ++ SAAAL   D++F QYRE GVL++R + L+ F  QC+ N  D GKGRQMP HYG  + ++VT++S +A                                        TQIPQAVGAAY+ K    +   I YFG+G +SE
Sbjct:   58 SAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLA----------------------------------------TQIPQAVGAAYAAKRANANRVVICYFGEGAASE 243

[Close]

BLAST of GT621396 vs. SwissProt
Match: ODBA_HUMAN (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Homo sapiens GN=BCKDHA PE=1 SV=2)

HSP 1 Score: 163.696 bits (413), Expect = 1.058e-39
Identity = 88/226 (38.94%), Postives = 123/226 (54.42%), Query Frame = 3

Query:    6 NVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            +  FI +++F+  + +  IP YRV+D  G  I  +    + ++  +K+Y  M  L TMD I YE+QRQGRISFY+T  GEE  ++ SAAAL   D++F QYRE GVL++R + L+ F  QC+ N  D GKGRQMP HYG  + ++VT++S +A                                        TQIPQAVGAAY+ K    +   I YFG+G +SE
Sbjct:   58 SAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLA----------------------------------------TQIPQAVGAAYAAKRANANRVVICYFGEGAASE 243

[Close]

BLAST of GT621396 vs. SwissProt
Match: ODBA_BOVIN (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Bos taurus GN=BCKDHA PE=2 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 1.382e-39
Identity = 88/226 (38.94%), Postives = 122/226 (53.98%), Query Frame = 3

Query:    6 NVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            +  FI +++F+  + +  IP YRV+D  G  I  +    + ++  +K Y  M  L TMD I YE+QRQGRISFY+T  GEE  ++ SAAAL   D++F QYRE GVL++R + L+ F  QC+ N  D GKGRQMP HYG  + ++VT++S +A                                        TQIPQAVGAAY+ K    +   I YFG+G +SE
Sbjct:   68 SAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPQEKVLKFYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDDTDLVFGQYREAGVLMYRDYPLELFMAQCYGNVSDLGKGRQMPVHYGCRERHFVTISSPLA----------------------------------------TQIPQAVGAAYAAKRANANRVVICYFGEGAASE 253

[Close]

BLAST of GT621396 vs. SwissProt
Match: ODBA_THET8 (2-oxoisovalerate dehydrogenase subunit alpha OS=Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) GN=TTHA0229 PE=1 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.537e-17
Identity = 62/205 (30.24%), Postives = 92/205 (44.88%), Query Frame = 3

Query:   72 RVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMD-DVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            R++ + G+  LG   + +  +   ++Y DM+A + +D  +    R G+ SF   A G EA  +A A A+    D +FP YR+ G+ L  G  L+E   Q  + K D  KGRQMP H GS   N+ TVAS +A                                        + +P A GAA S+K+ +    A+  FGDG +SE
Sbjct:   17 RLIGEEGEW-LGDFPLDLEGEKLRRLYRDMLAARMLDERYTILIRTGKTSFIAPAAGHEAAQVAIAHAIRPGFDWVFPYYRDHGLALALGIPLKELLGQMLATKADPNKGRQMPEHPGSKALNFFTVASPIA----------------------------------------SHVPPAAGAAISMKLLRTGQVAVCTFGDGATSE 180

[Close]

BLAST of GT621396 vs. SwissProt
Match: ODBA_THET2 (2-oxoisovalerate dehydrogenase subunit alpha OS=Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) GN=TT_C1757 PE=3 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 3.313e-17
Identity = 62/205 (30.24%), Postives = 92/205 (44.88%), Query Frame = 3

Query:   72 RVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMD-DVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            R++ + G+  LG   + +  +   ++Y DM+A + +D  +    R G+ SF   A G EA  +A A A+    D +FP YR+ G+ L  G  L+E   Q  + K D  KGRQMP H GS   N+ TVAS +A                                        + +P A GAA S+K+ +    A+  FGDG +SE
Sbjct:   17 RLIGEEGEW-LGDFPLDLEGEKLRRLYRDMLAARMLDERYTILIRTGKTSFIAPAAGHEAAQVAIAHAIRPGFDWVFPYYRDHGLALALGIPLKELFGQMLATKADPNKGRQMPEHPGSKALNFFTVASPIA----------------------------------------SHVPPAAGAAISMKLLRTGQVAVCTFGDGATSE 180

[Close]

BLAST of GT621396 vs. SwissProt
Match: ODBA_BACSU (2-oxoisovalerate dehydrogenase subunit alpha OS=Bacillus subtilis GN=bfmBAA PE=1 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 9.640e-17
Identity = 56/189 (29.63%), Postives = 87/189 (46.03%), Query Frame = 3

Query:  123 VSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMD-DVIFPQYREQGVLLWRGFTLQEFANQCFSNKFD-NGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            +++  AV MY  M+  + +D   +   R G+I F ++  G+EA  + +A AL  + D + P YR+ GV+L  G T ++     F+   D N  GRQMP H+G  K+  VT +S V                                        +TQ+P AVG A + +M+KKD  A   FG+G S++
Sbjct:   11 LTDQEAVDMYRTMLLARKIDERMWLLNRSGKIPFVISCQGQEAAQVGAAFALDREMDYVLPYYRDMGVVLAFGMTAKDLMMSGFAKAADPNSGGRQMPGHFGQKKNRIVTGSSPV----------------------------------------TTQVPHAVGIALAGRMEKKDIAAFVTFGEGSSNQ 159

[Close]

BLAST of GT621396 vs. TAIR peptide
Match: AT5G09300.2 (| Symbols: | Thiamin diphosphate-binding fold (THDP-binding) superfamily protein | chr5:2884282-2886291 REVERSE LENGTH=401)

HSP 1 Score: 275.404 bits (703), Expect = 2.484e-74
Identity = 142/227 (62.56%), Postives = 160/227 (70.48%), Query Frame = 3

Query:    3 GNVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            G V F P+++F+S S  +R+PCYRVLDDNG  I  +  VQVSE+VAVK+Y+DMV LQ MD IFYEAQRQGR+SFY TA GEEAINIASAAAL+  DVIFPQYRE GVLLWRGFTLQEFANQCF NK D GKGRQMP HYGSNK NY TV++T+A                                        TQ+P AVGAAYSLKMDKKDACA+TYFGDGG+SE
Sbjct:   14 GKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIA----------------------------------------TQLPNAVGAAYSLKMDKKDACAVTYFGDGGTSE 200

[Close]

BLAST of GT621396 vs. TAIR peptide
Match: AT5G09300.1 (| Symbols: | Thiamin diphosphate-binding fold (THDP-binding) superfamily protein | chr5:2884282-2886797 REVERSE LENGTH=472)

HSP 1 Score: 275.404 bits (703), Expect = 2.484e-74
Identity = 142/227 (62.56%), Postives = 160/227 (70.48%), Query Frame = 3

Query:    3 GNVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            G V F P+++F+S S  +R+PCYRVLDDNG  I  +  VQVSE+VAVK+Y+DMV LQ MD IFYEAQRQGR+SFY TA GEEAINIASAAAL+  DVIFPQYRE GVLLWRGFTLQEFANQCF NK D GKGRQMP HYGSNK NY TV++T+A                                        TQ+P AVGAAYSLKMDKKDACA+TYFGDGG+SE
Sbjct:   85 GKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIA----------------------------------------TQLPNAVGAAYSLKMDKKDACAVTYFGDGGTSE 271

[Close]

BLAST of GT621396 vs. TAIR peptide
Match: AT1G21400.1 (| Symbols: | Thiamin diphosphate-binding fold (THDP-binding) superfamily protein | chr1:7493492-7496240 FORWARD LENGTH=472)

HSP 1 Score: 244.202 bits (622), Expect = 6.133e-65
Identity = 126/227 (55.51%), Postives = 149/227 (65.64%), Query Frame = 3

Query:    3 GNVNFIPQMKFLSASTLDRIPCYRVLDDNGDTILGTHSVQVSEDVAVKMYNDMVALQTMDTIFYEAQRQGRISFYVTANGEEAINIASAAALSMDDVIFPQYREQGVLLWRGFTLQEFANQCFSNKFDNGKGRQMPAHYGSNKHNYVTVASTVA*VLKILIYVXXXXXXXXXXXXFSPMIMKVAK*KIYFEICSTQIPQAVGAAYSLKMDKKDACAITYFGDGGSSE 683
            G V +  +MKF+  S+  RIPCYRVLD++G  I  +  + VSE +AV+MY  M  LQ MD IFYEAQRQGRISFY+T+ GEEAINIASAAALS DDV+ PQYRE GVLLWRGFTL+EFANQCF NK D GKGRQMP HYGSN+ NY T++S +A                                        TQ+PQA G  YSLKMDKK+AC +T+ GDGG+SE
Sbjct:   85 GKVGYTSEMKFIPESSSRRIPCYRVLDEDGRIIPDSDFIPVSEKLAVRMYEQMATLQVMDHIFYEAQRQGRISFYLTSVGEEAINIASAAALSPDDVVLPQYREPGVLLWRGFTLEEFANQCFGNKADYGKGRQMPIHYGSNRLNYFTISSPIA----------------------------------------TQLPQAAGVGYSLKMDKKNACTVTFIGDGGTSE 271

The following BLAST results are available for this feature:

BLAST of GT621396 vs. TrEMBL
BLAST of GT621396 vs. SwissProt
BLAST of GT621396 vs. TAIR peptide

BLAST of GT621396 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9FY85_ARATH	3.844e-72	62.56	Branched-chain alpha keto-acid dehydrogenase E1 al...	[more]
Q84JL2_ARATH	3.844e-72	62.56	Putative branched-chain alpha keto-acid dehydrogen...	[more]
B4F923_MAIZE	5.021e-72	62.11	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
D7M224_ARALY	6.557e-72	62.11	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9H7L5_POPTR	3.254e-71	59.91	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7U4C7_VITVI	9.468e-71	63.72	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9RRD6_RICCO	2.109e-70	62.11	2-oxoisovalerate dehydrogenase, putative OS=Ricinu...	[more]
Q2QWT8_ORYSJ	2.755e-70	60.35	2-oxoisovalerate dehydrogenase alpha subunit, mito...	[more]
Q2QWT6_ORYSJ	2.755e-70	60.35	2-oxoisovalerate dehydrogenase alpha subunit, mito...	[more]
B9GC70_ORYSJ	2.755e-70	60.35	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of GT621396 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ODBA_DICDI	1.131e-41	42.31	2-oxoisovalerate dehydrogenase subunit alpha, mito...	[more]
ODBA_RAT	4.751e-40	38.50	2-oxoisovalerate dehydrogenase subunit alpha, mito...	[more]
ODBA_PANTR	1.058e-39	38.94	2-oxoisovalerate dehydrogenase subunit alpha, mito...	[more]
ODBA_MOUSE	1.058e-39	38.05	2-oxoisovalerate dehydrogenase subunit alpha, mito...	[more]
ODBA_MACFA	1.058e-39	38.94	2-oxoisovalerate dehydrogenase subunit alpha, mito...	[more]
ODBA_HUMAN	1.058e-39	38.94	2-oxoisovalerate dehydrogenase subunit alpha, mito...	[more]
ODBA_BOVIN	1.382e-39	38.94	2-oxoisovalerate dehydrogenase subunit alpha, mito...	[more]
ODBA_THET8	2.537e-17	30.24	2-oxoisovalerate dehydrogenase subunit alpha OS=Th...	[more]
ODBA_THET2	3.313e-17	30.24	2-oxoisovalerate dehydrogenase subunit alpha OS=Th...	[more]
ODBA_BACSU	9.640e-17	29.63	2-oxoisovalerate dehydrogenase subunit alpha OS=Ba...	[more]

BLAST of GT621396 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT5G09300.2	2.484e-74	62.56	\| Symbols: \| Thiamin diphosphate-binding fold (TH...	[more]
AT5G09300.1	2.484e-74	62.56	\| Symbols: \| Thiamin diphosphate-binding fold (TH...	[more]
AT1G21400.1	6.133e-65	55.51	\| Symbols: \| Thiamin diphosphate-binding fold (TH...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 19..30 score
None	No IPR available	SEG	seg	seg	coord: 7..15 score
None	No IPR available	GENE3D	3.40.50.970	no description	coord: 3..154 score: 3
None	No IPR available	PANTHER	PTHR11516	PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT (BACTERIAL AND ORGANELLAR)	coord: 25..154 score: 7.5
None	No IPR available	PANTHER	PTHR11516:SF1	2-OXOISOVALERATE DEHYDROGENASE ALPHA SUBUNIT-RELATED	coord: 25..154 score: 7.5
None	No IPR available	SUPERFAMILY	52518	Thiamin diphosphate-binding fold (THDP-binding)	coord: 8..154 score: 2.4
None	No IPR available	GENE3D	3.40.50.970	no description	coord: 6..41 score: 3.9
None	No IPR available	PANTHER	PTHR11516	PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT (BACTERIAL AND ORGANELLAR)	coord: 8..41 score: 4.8
None	No IPR available	PANTHER	PTHR11516:SF1	2-OXOISOVALERATE DEHYDROGENASE ALPHA SUBUNIT-RELATED	coord: 8..41 score: 4.8
None	No IPR available	SUPERFAMILY	52518	Thiamin diphosphate-binding fold (THDP-binding)	coord: 8..41 score: 1.5
IPR001017	Dehydrogenase, E1 component	PFAM	PF00676	E1_dh	coord: 53..153 score: 1.2
IPR001017	Dehydrogenase, E1 component	PFAM	PF00676	E1_dh	coord: 6..41 score: 2.4

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT621396 ID=GT621396; Name=GT621396; organism=Lens culinaris; type=EST; length=785bp
GAGGAAATGTCAACTTCATCCCTCAAATGAAGTTTTTATCTGCATCAACG
CTAGACAGAATCCCTTGTTACCGTGTTCTTGATGACAATGGGGACACAAT
TTTGGGGACTCACTCTGTGCAGGTTAGTGAGGATGTTGCTGTTAAAATGT
ATAATGACATGGTTGCACTTCAAACTATGGATACTATCTTCTATGAAGCG
CAAAGACAAGGAAGAATCTCGTTTTATGTAACGGCAAATGGGGAAGAGGC
TATTAACATTGCTTCTGCTGCAGCTCTTTCCATGGATGATGTTATTTTCC
CTCAGTATAGGGAACAGGGAGTCTTGTTATGGCGTGGTTTCACTCTGCAA
GAATTTGCAAATCAATGTTTTTCCAATAAGTTTGATAATGGGAAAGGAAG
GCAGATGCCGGCCCATTATGGGTCTAACAAGCACAACTATGTGACTGTAG
CATCAACCGTTGCGTAAGTATTGAAAATTTTGATCTATGTATGATAATTA
CTACTATTATTAATGTTGTTGCTGCTGTTTTCCCCAATGATTATGAAAGT
TGCAAAATGAAAGATTTACTTTGAGATCTGTAGTACGCAAATTCCCCAAG
CTGTTGGAGCTGCATATTCCTTAAAGATGGACAAAAAGGATGCATGTGCC
ATAACTTACTTCGGAGATGGCGGTTCAAGTGAGGTAAACTGTACCCTTTT
TTCTATAATAGTAAGACTCATTTCCCTCATTTCTGAAAGGAGATTTCTGT
GTGATTTAAACATTGCATGGATTGATATCTTTAGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR001017	DH_E1

Search form