GT623149

EST Overview


Name	GT623149
Unique Name	GT623149
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	839

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
KEGG analysis for Lens culinaris unigene v1	2011-01-05
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT623149 vs. TrEMBL
Match: D7SHG7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_0.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00007619001 PE=4 SV=1)

HSP 1 Score: 310.842 bits (795), Expect = 9.312e-83
Identity = 150/166 (90.36%), Postives = 158/166 (95.18%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPN--SYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDWPVGATSRESIKAVTK 838
            MHDL +IHTDLKPENILLVSP+YVKVPDYK+SSRSP   SYFK+VPKSSAIKVIDFGSTTYER DQ+YIVSTRHYRAPEVILGLGWS+PCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLP HMLK+VDRHAEKYVRRG+LDWP GATSRESIKAV K
Sbjct:  216 MHDLRLIHTDLKPENILLVSPEYVKVPDYKVSSRSPKDGSYFKRVPKSSAIKVIDFGSTTYERQDQNYIVSTRHYRAPEVILGLGWSYPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPQHMLKRVDRHAEKYVRRGRLDWPEGATSRESIKAVLK 381

HSP 2 Score: 111.309 bits (277), Expect = 1.083e-22
Identity = 53/60 (88.33%), Postives = 58/60 (96.67%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            KI+SKMGEGTFGQVLECWDRE+KEMVA+KIVRGIKKYREAAMIEIE+LQQL K+D GGNR
Sbjct:  101 KINSKMGEGTFGQVLECWDRERKEMVAVKIVRGIKKYREAAMIEIEVLQQLAKHDKGGNR 160

HSP 3 Score: 58.9214 bits (141), Expect = 6.378e-7
Identity = 29/35 (82.86%), Postives = 30/35 (85.71%), Query Frame = 2

Query:   65 MEM-ERVFEFPHTHMDRRPRKRARLGWDV-PEVPK 163
            MEM ERV EFPHTHMDRRPRKR RLGWDV P+ PK
Sbjct:    1 MEMVERVTEFPHTHMDRRPRKRPRLGWDVLPQAPK 35

[Close]

BLAST of GT623149 vs. TrEMBL
Match: B9RAS6_RICCO (Afc, putative OS=Ricinus communis GN=RCOM_1508280 PE=4 SV=1)

HSP 1 Score: 308.145 bits (788), Expect = 6.036e-82
Identity = 148/166 (89.16%), Postives = 158/166 (95.18%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSP--NSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDWPVGATSRESIKAVTK 838
            MHDL +IHTDLKPENILLVSPDYVKVPDYK S+RSP  +SYFK+VPKSSA+KVIDFGSTTYER DQ+YIVSTRHYRAPEVILGLGWS+PCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLP HMLK++DRHAEKYVRRG+LDWP GATSRESIKAV K
Sbjct:  217 MHDLHLIHTDLKPENILLVSPDYVKVPDYKGSTRSPKDSSYFKRVPKSSAVKVIDFGSTTYERQDQNYIVSTRHYRAPEVILGLGWSYPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPQHMLKRIDRHAEKYVRRGRLDWPEGATSRESIKAVLK 382

HSP 2 Score: 115.546 bits (288), Expect = 5.742e-24
Identity = 55/60 (91.67%), Postives = 59/60 (98.33%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            KIHSKMGEGTFGQVLECWDRE+KEMVAIKIVRGIKKYREAAMIEI++LQQLGK+D GGNR
Sbjct:  102 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIKKYREAAMIEIDVLQQLGKHDKGGNR 161

HSP 3 Score: 62.003 bits (149), Expect = 7.534e-8
Identity = 28/33 (84.85%), Postives = 29/33 (87.88%), Query Frame = 2

Query:   65 MEMERVFEFPHTHMDRRPRKRARLGWDVPEVPK 163
            MEMERV EFP  H+DRRPRKRARLGWDVP VPK
Sbjct:    1 MEMERVTEFPLPHLDRRPRKRARLGWDVPLVPK 33

[Close]

BLAST of GT623149 vs. TrEMBL
Match: C6T8M4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 307.375 bits (786), Expect = 1.030e-81
Identity = 148/162 (91.36%), Postives = 155/162 (95.68%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDWPVGATSRESIKAV 832
            MHDL MIHTDLKPENILLVSP+YVKVPDYK SSRSP+SYFK+VPKSSAIKVIDFGSTTYER DQ+YIVSTRHYRAPEVILGLGWS+PCDIWSVGCILVELCTG ALFQTHENLEHLAMMERVLGPLP  MLK+VDRHAEKYVRRG+LDWP GATSRESIKAV
Sbjct:  214 MHDLRMIHTDLKPENILLVSPEYVKVPDYKSSSRSPSSYFKRVPKSSAIKVIDFGSTTYEREDQNYIVSTRHYRAPEVILGLGWSYPCDIWSVGCILVELCTGGALFQTHENLEHLAMMERVLGPLPQPMLKRVDRHAEKYVRRGRLDWPEGATSRESIKAV 375

HSP 2 Score: 116.316 bits (290), Expect = 3.366e-24
Identity = 55/60 (91.67%), Postives = 59/60 (98.33%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            KIHSKMGEGTFGQVLECWDRE+KEMVA+KIVRGIKKYREAAMIEIE+LQQLGK+D GGNR
Sbjct:   99 KIHSKMGEGTFGQVLECWDRERKEMVAVKIVRGIKKYREAAMIEIEVLQQLGKHDKGGNR 158

HSP 3 Score: 75.485 bits (184), Expect = 6.585e-12
Identity = 32/33 (96.97%), Postives = 33/33 (100.00%), Query Frame = 2

Query:   65 MEMERVFEFPHTHMDRRPRKRARLGWDVPEVPK 163
            MEMERVFEFPHTHMDRRPRKRARLGWD+PEVPK
Sbjct:    1 MEMERVFEFPHTHMDRRPRKRARLGWDIPEVPK 33

[Close]

BLAST of GT623149 vs. TrEMBL
Match: C6TMJ6_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 304.294 bits (778), Expect = 8.716e-81
Identity = 143/164 (87.20%), Postives = 156/164 (95.12%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDWPVGATSRESIKAVTK 838
            MHDL MIHTDLKPENILLVSP+Y+K+PDYK ++RSP+S+FK+VPKSSAIKVIDFGSTTYER DQ+YIVSTRHYRAPEVILGLGWS+PCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGP+P  MLK+VDRHAEKYVRRG+LDWP GA SRESIKAV K
Sbjct:  209 MHDLHMIHTDLKPENILLVSPEYLKIPDYKSTTRSPSSFFKRVPKSSAIKVIDFGSTTYEREDQTYIVSTRHYRAPEVILGLGWSYPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPIPQQMLKRVDRHAEKYVRRGRLDWPEGAASRESIKAVMK 372

HSP 2 Score: 112.464 bits (280), Expect = 4.861e-23
Identity = 53/60 (88.33%), Postives = 57/60 (95.00%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            KIH KMGEGTFGQVLECWDRE+KEMVAIKIVRGIKKYREAAM+EIE+LQQLGK+D G NR
Sbjct:   94 KIHGKMGEGTFGQVLECWDRERKEMVAIKIVRGIKKYREAAMVEIEVLQQLGKHDKGSNR 153

HSP 3 Score: 64.3142 bits (155), Expect = 1.518e-8
Identity = 27/33 (81.82%), Postives = 30/33 (90.91%), Query Frame = 2

Query:   65 MEMERVFEFPHTHMDRRPRKRARLGWDVPEVPK 163
            MEMER+ EFPHT+MDR PRKRARLGWD+ EVPK
Sbjct:    1 MEMERIVEFPHTYMDRPPRKRARLGWDIAEVPK 33

[Close]

BLAST of GT623149 vs. TrEMBL
Match: C6T7I2_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 303.138 bits (775), Expect = 1.942e-80
Identity = 147/164 (89.63%), Postives = 154/164 (93.90%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDWPVGATSRESIKAVTK 838
            MHDL MIHTDLKPENILLVSP+YVKVPDYK SSRS  SYFK+VPKSSAIKVIDFGSTTYER DQ+YIVSTRHYRAPEVILGLGWS+PCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLG LP  M+K+VDRHAEKYVRRG+LDWP GATSRESIKAV K
Sbjct:  214 MHDLRMIHTDLKPENILLVSPEYVKVPDYKSSSRSSCSYFKRVPKSSAIKVIDFGSTTYEREDQNYIVSTRHYRAPEVILGLGWSYPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGSLPQTMMKRVDRHAEKYVRRGRLDWPEGATSRESIKAVMK 377

HSP 2 Score: 115.161 bits (287), Expect = 7.499e-24
Identity = 54/60 (90.00%), Postives = 59/60 (98.33%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            KIH+KMGEGTFGQVLECWDRE+KEMVA+KIVRGIKKYREAAMIEIE+LQQLGK+D GGNR
Sbjct:   99 KIHTKMGEGTFGQVLECWDRERKEMVAVKIVRGIKKYREAAMIEIEVLQQLGKHDKGGNR 158

HSP 3 Score: 73.1738 bits (178), Expect = 3.268e-11
Identity = 31/33 (93.94%), Postives = 33/33 (100.00%), Query Frame = 2

Query:   65 MEMERVFEFPHTHMDRRPRKRARLGWDVPEVPK 163
            MEMERVFEFPHT+MDRRPRKRARLGWD+PEVPK
Sbjct:    1 MEMERVFEFPHTYMDRRPRKRARLGWDIPEVPK 33

[Close]

BLAST of GT623149 vs. TrEMBL
Match: B9IF06_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_824707 PE=4 SV=1)

HSP 1 Score: 298.901 bits (764), Expect = 3.662e-79
Identity = 144/166 (86.75%), Postives = 156/166 (93.98%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSP--NSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDWPVGATSRESIKAVTK 838
            MHDL MIHTDLKPENILLVS DYVKVPDYK S+RSP  +SY+K+VPKSSAIKVIDFGSTTYER DQ+YIVSTRHYRAPEVILGLGWS+PCD+WSVGCILVELCTGEALFQTHENLEHLAMMERVLGP+P +MLK+VDRHAEK+VRRG+LDWP GA SRESIKAV K
Sbjct:  209 MHDLHMIHTDLKPENILLVSSDYVKVPDYKNSTRSPKDSSYYKRVPKSSAIKVIDFGSTTYERQDQNYIVSTRHYRAPEVILGLGWSYPCDVWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPMPQNMLKRVDRHAEKFVRRGRLDWPEGAASRESIKAVLK 374

HSP 2 Score: 114.39 bits (285), Expect = 1.279e-23
Identity = 54/60 (90.00%), Postives = 59/60 (98.33%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            KIH+KMGEGTFGQVLECWDRE+KEMVAIKIVRGIKKY+EAAMIEIE+LQQLGK+D GGNR
Sbjct:   94 KIHNKMGEGTFGQVLECWDRERKEMVAIKIVRGIKKYKEAAMIEIEVLQQLGKHDKGGNR 153

[Close]

BLAST of GT623149 vs. TrEMBL
Match: B9I3I2_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_423025 PE=4 SV=1)

HSP 1 Score: 297.36 bits (760), Expect = 1.065e-78
Identity = 144/166 (86.75%), Postives = 153/166 (92.17%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPN--SYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDWPVGATSRESIKAVTK 838
            MHDL MIHTDLKPENILLVS DYVKVPDYK SSRSP   SY+K+VPKSSAIKVIDFGSTTYER DQ+YIVSTRHYRAPEVILGLGWS+PCDIWS GCILVELCTGEALFQTHENLEHLAMMERVLGP+P H+LK+VDRHA KYVRRG+LDWP GA SRES+KAV K
Sbjct:  209 MHDLRMIHTDLKPENILLVSSDYVKVPDYKNSSRSPKDISYYKRVPKSSAIKVIDFGSTTYERQDQNYIVSTRHYRAPEVILGLGWSYPCDIWSAGCILVELCTGEALFQTHENLEHLAMMERVLGPMPQHILKRVDRHAGKYVRRGRLDWPEGAASRESMKAVLK 374

HSP 2 Score: 112.464 bits (280), Expect = 4.861e-23
Identity = 54/60 (90.00%), Postives = 57/60 (95.00%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            KIHSKMGEGTFGQVLECWDRE+KEMVAIKIVRGIKKYREAAMIEIE+LQ LGK+D G NR
Sbjct:   94 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIKKYREAAMIEIEVLQNLGKHDKGSNR 153

[Close]

BLAST of GT623149 vs. TrEMBL
Match: Q9M598_MESCR (Protein kinase MK5 OS=Mesembryanthemum crystallinum PE=2 SV=1)

HSP 1 Score: 294.278 bits (752), Expect = 9.019e-78
Identity = 142/166 (85.54%), Postives = 154/166 (92.77%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSP--NSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDWPVGATSRESIKAVTK 838
            MHDL +IHTDLKPEN+LLVS DYVK+ DYK  SRSP  +SY+KKVPKSSAIKVIDFGSTTYER DQ+YIVSTRHYRAPEVILGLGWS+PCD+WSVGCILVELC+GEALFQTHENLE LAMMERVLGPLP HMLK+VDRHAEKYVRRG+LDWP GA SRESI+AVTK
Sbjct:  219 MHDLHLIHTDLKPENVLLVSSDYVKIRDYKGFSRSPKDSSYYKKVPKSSAIKVIDFGSTTYERPDQNYIVSTRHYRAPEVILGLGWSYPCDVWSVGCILVELCSGEALFQTHENLEDLAMMERVLGPLPQHMLKRVDRHAEKYVRRGRLDWPEGAASRESIRAVTK 384

HSP 2 Score: 112.079 bits (279), Expect = 6.348e-23
Identity = 53/60 (88.33%), Postives = 58/60 (96.67%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            KIH KMGEGTFGQVLECWDRE+KEMVAIKIVRGIKKYREAAMIEI++LQQLGK+D GG+R
Sbjct:  104 KIHGKMGEGTFGQVLECWDRERKEMVAIKIVRGIKKYREAAMIEIDVLQQLGKHDKGGSR 163

[Close]

BLAST of GT623149 vs. TrEMBL
Match: A7XW60_SORBI (Lammer-type protein kinase OS=Sorghum bicolor GN=AFC1 PE=2 SV=1)

HSP 1 Score: 294.278 bits (752), Expect = 9.019e-78
Identity = 136/166 (81.93%), Postives = 156/166 (93.98%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPN--SYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDWPVGATSRESIKAVTK 838
            MH+L +IHTDLKPENILLVSP+Y+KVPDYK+SSRSP   SY+K++PKSSAIKVIDFGSTTY++ DQSY+VSTRHYRAPEVILGLGWS+PCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLP HMLK+ DRH++KY+R+G+L+WP G TSRES+KAV K
Sbjct:  111 MHELRLIHTDLKPENILLVSPEYIKVPDYKVSSRSPKEGSYYKRLPKSSAIKVIDFGSTTYDQQDQSYVVSTRHYRAPEVILGLGWSYPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPYHMLKRADRHSDKYIRKGRLNWPEGCTSRESMKAVMK 276

HSP 2 Score: 92.8189 bits (229), Expect = 3.986e-17
Identity = 43/50 (86.00%), Postives = 48/50 (96.00%), Query Frame = 1

Query:  181 MGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKND 330
            MGEGTFGQVLECWD+E+KEMVAIKI+RGIKKYR+AAMIEI ML+QLGK D
Sbjct:    1 MGEGTFGQVLECWDKERKEMVAIKIIRGIKKYRDAAMIEIGMLEQLGKYD 50

[Close]

BLAST of GT623149 vs. TrEMBL
Match: B7FLL1_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 293.123 bits (749), Expect = 2.009e-77
Identity = 139/164 (84.76%), Postives = 153/164 (93.29%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDWPVGATSRESIKAVTK 838
            MHDL MIHTDLKPENILLVS +Y+K+PDYK SSR+P S++K+VPKSSAIKVIDFGSTTYER +Q+YIVSTRHYRAPEVILGLGWS+PCD+WSVGCILVELCTGEALFQTHENLEHLAMMERVLG LP  +LK+VDRHAEKYVRRG+LDWP GA SRESIKAV K
Sbjct:  111 MHDLHMIHTDLKPENILLVSSEYLKIPDYKSSSRAPCSFYKRVPKSSAIKVIDFGSTTYERENQNYIVSTRHYRAPEVILGLGWSYPCDVWSVGCILVELCTGEALFQTHENLEHLAMMERVLGLLPQQLLKRVDRHAEKYVRRGRLDWPEGANSRESIKAVMK 274

HSP 2 Score: 105.531 bits (262), Expect = 5.942e-21
Identity = 50/55 (90.91%), Postives = 53/55 (96.36%), Query Frame = 1

Query:  181 MGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            MGEGTFGQVLECWDRE+KEMVAIK+VR IKKYREAAMIEIEMLQQLGK+D GGNR
Sbjct:    1 MGEGTFGQVLECWDRERKEMVAIKVVRAIKKYREAAMIEIEMLQQLGKHDKGGNR 55

[Close]

BLAST of GT623149 vs. SwissProt
Match: AFC2_ARATH (Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana GN=AFC2 PE=1 SV=1)

HSP 1 Score: 286.574 bits (732), Expect = 1.204e-76
Identity = 136/164 (82.93%), Postives = 149/164 (90.85%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDWPVGATSRESIKAVTK 838
            MHDL MIHTDLKPENILLVS DYVK+P+YK S    +  +K+VPKSSAIKVIDFGSTTYER DQ+YIVSTRHYRAPEVILGLGWS+PCD+WSVGCI+VELCTGEALFQTHENLEHLAMMERVLGP P  MLKKVDRH+EKYVRRG+LDWP GATSR+S+KAV K
Sbjct:  214 MHDLRMIHTDLKPENILLVSSDYVKIPEYKGSRLQRDVCYKRVPKSSAIKVIDFGSTTYERQDQTYIVSTRHYRAPEVILGLGWSYPCDVWSVGCIIVELCTGEALFQTHENLEHLAMMERVLGPFPQQMLKKVDRHSEKYVRRGRLDWPDGATSRDSLKAVLK 377

HSP 2 Score: 115.161 bits (287), Expect = 4.799e-25
Identity = 54/60 (90.00%), Postives = 59/60 (98.33%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            KI+SKMGEGTFGQVLECWDRE+KEMVA+KIVRG+KKYREAAMIEIEMLQQLGK+D GGNR
Sbjct:   99 KIYSKMGEGTFGQVLECWDRERKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNR 158

HSP 3 Score: 62.7734 bits (151), Expect = 2.827e-9
Identity = 29/34 (85.29%), Postives = 30/34 (88.24%), Query Frame = 2

Query:   65 MEMERVFEFPHTHMDRRPRKRARLGWDV-PEVPK 163
            MEMERV EFPHTHMDRRPRKRARLGWDV P+  K
Sbjct:    1 MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATK 34

[Close]

BLAST of GT623149 vs. SwissProt
Match: AFC1_ARATH (Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana GN=AFC1 PE=2 SV=2)

HSP 1 Score: 267.314 bits (682), Expect = 7.556e-71
Identity = 127/167 (76.05%), Postives = 146/167 (87.43%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSR--SPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRG-KLDWPVGATSRESIKAVTK 838
            MHDL +IHTDLKPENILLVS +Y+K+PDYK  SR     SYFK +PKSSAIK+IDFGSTT+E  D +YIVSTRHYRAPEVILG+GW++PCD+WS+GCILVELC+GEALFQTHENLEHLAMMERVLGPLP HM+ + DR +EKY RRG KLDWP GATSR+S+KAV K
Sbjct:  231 MHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVGWNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFRRGAKLDWPEGATSRDSLKAVWK 397

HSP 2 Score: 89.7373 bits (221), Expect = 2.159e-17
Identity = 41/60 (68.33%), Postives = 54/60 (90.00%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            +I SKMGEGTFGQVLEC+D + KE+VAIK++R I KYREAAMIEI++LQ+L ++D+GG+R
Sbjct:  116 QILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSR 175

[Close]

BLAST of GT623149 vs. SwissProt
Match: AFC3_ARATH (Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana GN=AFC3 PE=2 SV=2)

HSP 1 Score: 226.868 bits (577), Expect = 1.132e-58
Identity = 111/165 (67.27%), Postives = 134/165 (81.21%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRG-KLDWPVGATSRESIKAVTK 838
            MH+L ++HTDLKPENILLVS + VK+PD K S+ +  ++F+ +PKSSAIK+IDFGST  +      IV TRHYR+PEVILGLGWS+ CD+WS+GCIL ELCTGEALFQTH+NLEHLAMMER LGPLP HM +K  R AEKY RRG +L+WP GA SRESI+AV +
Sbjct:  187 MHELQLVHTDLKPENILLVSSENVKLPDNKRSAAN-ETHFRCLPKSSAIKLIDFGSTVCDNRIHHSIVQTRHYRSPEVILGLGWSYQCDLWSIGCILFELCTGEALFQTHDNLEHLAMMERALGPLPEHMTRKASRGAEKYFRRGCRLNWPEGANSRESIRAVKR 350

HSP 2 Score: 90.8929 bits (224), Expect = 9.693e-18
Identity = 44/60 (73.33%), Postives = 52/60 (86.67%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            KI SKMGEGTFG+VLECWDR+ KE VAIKI+R IKKYR+AAMIEI++LQ+L K+D G  R
Sbjct:   72 KILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEIDVLQKLVKSDKGRTR 131

[Close]

BLAST of GT623149 vs. SwissProt
Match: LKH1_SCHPO (Dual specificity protein kinase lkh1 OS=Schizosaccharomyces pombe GN=lkh1 PE=1 SV=3)

HSP 1 Score: 174.096 bits (440), Expect = 8.706e-43
Identity = 85/161 (52.80%), Postives = 118/161 (73.29%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSP-NSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRR-GKLDWPVGATSRESI 823
            +H LG++HTDLKPEN+LLVS       +   + R P  +Y +KV  S  I++IDFGS T+E    S +VSTRHYRAPE+ILGLGWS+PCD+WS+GCILVEL TG+ALFQTHE+ EHL MME++LGP   +M+ +  R ++++ +  GK+ +P+  T ++SI
Sbjct:  479 LHSLGLVHTDLKPENVLLVS-------NASRTIRLPYRNYSQKVLNSCEIRLIDFGSATFEDEYHSSVVSTRHYRAPEIILGLGWSYPCDVWSIGCILVELFTGQALFQTHEDSEHLCMMEKILGPFDRNMISRSSRTSQRFFKSDGKVRYPLSNTPKKSI 632

HSP 2 Score: 55.8398 bits (133), Expect = 3.455e-7
Identity = 23/50 (46.00%), Postives = 36/50 (72.00%), Query Frame = 1

Query:  181 MGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKND 330
            +G GTFG+V++C+D+      AIK+ R I KYREA++IE+ +LQ +  +D
Sbjct:  368 LGHGTFGKVIQCYDQSTGRHCAIKVTRAIPKYREASLIELRVLQTIAHSD 417

[Close]

BLAST of GT623149 vs. SwissProt
Match: CLK2_HUMAN (Dual specificity protein kinase CLK2 OS=Homo sapiens GN=CLK2 PE=1 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 2.801e-41
Identity = 85/168 (50.60%), Postives = 112/168 (66.67%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDW----PVGATSRESIKAVTK 838
            +HD  + HTDLKPENIL V+ DY     Y +  +      ++  KS+A++V+DFGS T++    S IVSTRHYRAPEVIL LGWS PCD+WS+GCI+ E   G  LFQTH+N EHLAMMER+LGP+P  M++K  +  +KY  RG+LDW      G   RE+ K + +
Sbjct:  281 LHDNKLTHTDLKPENILFVNSDYELT--YNLEKKRD----ERSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYYVGFTLFQTHDNREHLAMMERILGPIPSRMIRKTRK--QKYFYRGRLDWDENTSAGRYVRENCKPLRR 440

HSP 2 Score: 54.6842 bits (130), Expect = 7.698e-7
Identity = 25/56 (44.64%), Postives = 42/56 (75.00%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWD-REKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKND 330
            +I S +GEGTFG+V++C D R     VA+KI++ ++KY+EAA +EI +L+++ + D
Sbjct:  164 EIVSTLGEGTFGRVVQCVDHRRGGARVALKIIKNVEKYKEAARLEINVLEKINEKD 219

[Close]

BLAST of GT623149 vs. SwissProt
Match: CLK2_MOUSE (Dual specificity protein kinase CLK2 OS=Mus musculus GN=Clk2 PE=2 SV=1)

HSP 1 Score: 168.703 bits (426), Expect = 3.658e-41
Identity = 85/168 (50.60%), Postives = 112/168 (66.67%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDW----PVGATSRESIKAVTK 838
            +HD  + HTDLKPENIL V+ DY     Y +  +      ++  KS+A++V+DFGS T++    S IVSTRHYRAPEVIL LGWS PCD+WS+GCI+ E   G  LFQTH+N EHLAMMER+LGP+P  M++K  +  +KY  RG+LDW      G   RE+ K + +
Sbjct:  280 LHDNKLTHTDLKPENILFVNSDYELT--YNLEKKRD----ERSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYYVGFTLFQTHDNREHLAMMERILGPVPSRMIRKTRK--QKYFYRGRLDWDENTSAGRYVRENCKPLRR 439

[Close]

BLAST of GT623149 vs. SwissProt
Match: CLK4_HUMAN (Dual specificity protein kinase CLK4 OS=Homo sapiens GN=CLK4 PE=1 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 5.282e-40
Identity = 79/149 (53.02%), Postives = 101/149 (67.79%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDW 793
            +H   + HTDLKPENIL V  DYV      +   S     ++  K++ IKV+DFGS TY+    S +VSTRHYRAPEVIL LGWS PCD+WS+GCIL+E   G  +FQTH++ EHLAMMER+LGP+P HM++K  +   KY    +LDW
Sbjct:  277 LHHNKLTHTDLKPENILFVKSDYV------VKYNSKMKRDERTLKNTDIKVVDFGSATYDDEHHSTLVSTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTVFQTHDSKEHLAMMERILGPIPQHMIQKTRK--RKYFHHNQLDW 417

[Close]

BLAST of GT623149 vs. SwissProt
Match: CLK4_MOUSE (Dual specificity protein kinase CLK4 OS=Mus musculus GN=Clk4 PE=1 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 6.898e-40
Identity = 79/149 (53.02%), Postives = 101/149 (67.79%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDW 793
            +H   + HTDLKPENIL V  DYV      +   S     ++  K++ IKV+DFGS TY+    S +VSTRHYRAPEVIL LGWS PCD+WS+GCIL+E   G  +FQTH++ EHLAMMER+LGP+P HM++K  +   KY    +LDW
Sbjct:  277 LHHNKLTHTDLKPENILFVKSDYV------VKYNSKMKRDERTLKNTDIKVVDFGSATYDDEHHSTLVSTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTVFQTHDSKEHLAMMERILGPIPAHMIQKTRK--RKYFHHNQLDW 417

[Close]

BLAST of GT623149 vs. SwissProt
Match: DOA_DROME (Serine/threonine-protein kinase Doa OS=Drosophila melanogaster GN=Doa PE=1 SV=2)

HSP 1 Score: 162.155 bits (409), Expect = 3.424e-39
Identity = 82/152 (53.95%), Postives = 104/152 (68.42%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAE---KYVRRGKLDW 793
            +HD  + HTDLKPENIL V  DY    ++KI     N   ++V K++ +++IDFGS T++    S IVSTRHYRAPEVIL LGWS PCD+WS+GCIL EL  G  LFQTH+N EHLAMMER+LG +P  M +    +++   KY   GKLDW
Sbjct:  596 LHDNRLTHTDLKPENILFVDSDYTSHYNHKI-----NREVRRV-KNTDVRLIDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCILFELYLGITLFQTHDNREHLAMMERILGQIPYRMARNHTLYSKTKTKYFYHGKLDW 741

HSP 2 Score: 56.225 bits (134), Expect = 2.646e-7
Identity = 24/55 (43.64%), Postives = 41/55 (74.55%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKND 330
            KI + +GEGTFG+V++  D E+   +A+KI++ ++KYREAA +EI  L+++ + D
Sbjct:  480 KIMATLGEGTFGRVVKVKDMERDYCMALKIIKNVEKYREAAKLEINALEKIAQKD 534

[Close]

BLAST of GT623149 vs. SwissProt
Match: CLK1_HUMAN (Dual specificity protein kinase CLK1 OS=Homo sapiens GN=CLK1 PE=1 SV=2)

HSP 1 Score: 159.844 bits (403), Expect = 1.699e-38
Identity = 78/149 (52.35%), Postives = 99/149 (66.44%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDW 793
            +H   + HTDLKPENIL V  DY +  + KI         ++   +  IKV+DFGS TY+    S +VSTRHYRAPEVIL LGWS PCD+WS+GCIL+E   G  +F TH++ EHLAMMER+LGPLP HM++K  +   KY    +LDW
Sbjct:  279 LHSNKLTHTDLKPENILFVQSDYTEAYNPKIKRD------ERTLINPDIKVVDFGSATYDDEHHSTLVSTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTVFPTHDSKEHLAMMERILGPLPKHMIQKTRK--RKYFHHDRLDW 419

[Close]

BLAST of GT623149 vs. TAIR peptide
Match: AT4G24740.1 (| Symbols: AFC2, AME1, FC2 | FUS3-complementing gene 2 | chr4:12754729-12757653 REVERSE LENGTH=427)

HSP 1 Score: 286.574 bits (732), Expect = 1.186e-77
Identity = 136/164 (82.93%), Postives = 149/164 (90.85%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDWPVGATSRESIKAVTK 838
            MHDL MIHTDLKPENILLVS DYVK+P+YK S    +  +K+VPKSSAIKVIDFGSTTYER DQ+YIVSTRHYRAPEVILGLGWS+PCD+WSVGCI+VELCTGEALFQTHENLEHLAMMERVLGP P  MLKKVDRH+EKYVRRG+LDWP GATSR+S+KAV K
Sbjct:  214 MHDLRMIHTDLKPENILLVSSDYVKIPEYKGSRLQRDVCYKRVPKSSAIKVIDFGSTTYERQDQTYIVSTRHYRAPEVILGLGWSYPCDVWSVGCIIVELCTGEALFQTHENLEHLAMMERVLGPFPQQMLKKVDRHSEKYVRRGRLDWPDGATSRDSLKAVLK 377

HSP 2 Score: 115.161 bits (287), Expect = 4.730e-26
Identity = 54/60 (90.00%), Postives = 59/60 (98.33%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            KI+SKMGEGTFGQVLECWDRE+KEMVA+KIVRG+KKYREAAMIEIEMLQQLGK+D GGNR
Sbjct:   99 KIYSKMGEGTFGQVLECWDRERKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNR 158

HSP 3 Score: 62.7734 bits (151), Expect = 2.786e-10
Identity = 29/34 (85.29%), Postives = 30/34 (88.24%), Query Frame = 2

Query:   65 MEMERVFEFPHTHMDRRPRKRARLGWDV-PEVPK 163
            MEMERV EFPHTHMDRRPRKRARLGWDV P+  K
Sbjct:    1 MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATK 34

[Close]

BLAST of GT623149 vs. TAIR peptide
Match: AT4G24740.2 (| Symbols: AFC2, AME1, FC2 | FUS3-complementing gene 2 | chr4:12754729-12756549 REVERSE LENGTH=324)

HSP 1 Score: 286.574 bits (732), Expect = 1.186e-77
Identity = 136/164 (82.93%), Postives = 149/164 (90.85%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRGKLDWPVGATSRESIKAVTK 838
            MHDL MIHTDLKPENILLVS DYVK+P+YK S    +  +K+VPKSSAIKVIDFGSTTYER DQ+YIVSTRHYRAPEVILGLGWS+PCD+WSVGCI+VELCTGEALFQTHENLEHLAMMERVLGP P  MLKKVDRH+EKYVRRG+LDWP GATSR+S+KAV K
Sbjct:  111 MHDLRMIHTDLKPENILLVSSDYVKIPEYKGSRLQRDVCYKRVPKSSAIKVIDFGSTTYERQDQTYIVSTRHYRAPEVILGLGWSYPCDVWSVGCIIVELCTGEALFQTHENLEHLAMMERVLGPFPQQMLKKVDRHSEKYVRRGRLDWPDGATSRDSLKAVLK 274

HSP 2 Score: 107.457 bits (267), Expect = 9.862e-24
Identity = 50/55 (90.91%), Postives = 54/55 (98.18%), Query Frame = 1

Query:  181 MGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            MGEGTFGQVLECWDRE+KEMVA+KIVRG+KKYREAAMIEIEMLQQLGK+D GGNR
Sbjct:    1 MGEGTFGQVLECWDRERKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNR 55

[Close]

BLAST of GT623149 vs. TAIR peptide
Match: AT3G53570.4 (| Symbols: AFC1, AME2, FC1 | FUS3-complementing gene 1 | chr3:19861449-19864125 REVERSE LENGTH=467)

HSP 1 Score: 267.314 bits (682), Expect = 7.447e-72
Identity = 127/167 (76.05%), Postives = 146/167 (87.43%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSR--SPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRG-KLDWPVGATSRESIKAVTK 838
            MHDL +IHTDLKPENILLVS +Y+K+PDYK  SR     SYFK +PKSSAIK+IDFGSTT+E  D +YIVSTRHYRAPEVILG+GW++PCD+WS+GCILVELC+GEALFQTHENLEHLAMMERVLGPLP HM+ + DR +EKY RRG KLDWP GATSR+S+KAV K
Sbjct:  231 MHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVGWNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFRRGAKLDWPEGATSRDSLKAVWK 397

HSP 2 Score: 89.7373 bits (221), Expect = 2.128e-18
Identity = 41/60 (68.33%), Postives = 54/60 (90.00%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            +I SKMGEGTFGQVLEC+D + KE+VAIK++R I KYREAAMIEI++LQ+L ++D+GG+R
Sbjct:  116 QILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSR 175

[Close]

BLAST of GT623149 vs. TAIR peptide
Match: AT3G53570.2 (| Symbols: AFC1, AME2, FC1 | FUS3-complementing gene 1 | chr3:19861449-19864125 REVERSE LENGTH=467)

HSP 1 Score: 267.314 bits (682), Expect = 7.447e-72
Identity = 127/167 (76.05%), Postives = 146/167 (87.43%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSR--SPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRG-KLDWPVGATSRESIKAVTK 838
            MHDL +IHTDLKPENILLVS +Y+K+PDYK  SR     SYFK +PKSSAIK+IDFGSTT+E  D +YIVSTRHYRAPEVILG+GW++PCD+WS+GCILVELC+GEALFQTHENLEHLAMMERVLGPLP HM+ + DR +EKY RRG KLDWP GATSR+S+KAV K
Sbjct:  231 MHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVGWNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFRRGAKLDWPEGATSRDSLKAVWK 397

HSP 2 Score: 89.7373 bits (221), Expect = 2.128e-18
Identity = 41/60 (68.33%), Postives = 54/60 (90.00%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            +I SKMGEGTFGQVLEC+D + KE+VAIK++R I KYREAAMIEI++LQ+L ++D+GG+R
Sbjct:  116 QILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSR 175

[Close]

BLAST of GT623149 vs. TAIR peptide
Match: AT3G53570.1 (| Symbols: AFC1, AME2, FC1 | FUS3-complementing gene 1 | chr3:19861449-19864125 REVERSE LENGTH=467)

HSP 1 Score: 267.314 bits (682), Expect = 7.447e-72
Identity = 127/167 (76.05%), Postives = 146/167 (87.43%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSR--SPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRG-KLDWPVGATSRESIKAVTK 838
            MHDL +IHTDLKPENILLVS +Y+K+PDYK  SR     SYFK +PKSSAIK+IDFGSTT+E  D +YIVSTRHYRAPEVILG+GW++PCD+WS+GCILVELC+GEALFQTHENLEHLAMMERVLGPLP HM+ + DR +EKY RRG KLDWP GATSR+S+KAV K
Sbjct:  231 MHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVGWNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFRRGAKLDWPEGATSRDSLKAVWK 397

HSP 2 Score: 89.7373 bits (221), Expect = 2.128e-18
Identity = 41/60 (68.33%), Postives = 54/60 (90.00%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            +I SKMGEGTFGQVLEC+D + KE+VAIK++R I KYREAAMIEI++LQ+L ++D+GG+R
Sbjct:  116 QILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSR 175

[Close]

BLAST of GT623149 vs. TAIR peptide
Match: AT3G53570.3 (| Symbols: AFC1, AME2, FC1 | FUS3-complementing gene 1 | chr3:19861449-19864083 REVERSE LENGTH=453)

HSP 1 Score: 267.314 bits (682), Expect = 7.447e-72
Identity = 127/167 (76.05%), Postives = 146/167 (87.43%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSR--SPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRG-KLDWPVGATSRESIKAVTK 838
            MHDL +IHTDLKPENILLVS +Y+K+PDYK  SR     SYFK +PKSSAIK+IDFGSTT+E  D +YIVSTRHYRAPEVILG+GW++PCD+WS+GCILVELC+GEALFQTHENLEHLAMMERVLGPLP HM+ + DR +EKY RRG KLDWP GATSR+S+KAV K
Sbjct:  217 MHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVGWNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFRRGAKLDWPEGATSRDSLKAVWK 383

HSP 2 Score: 89.7373 bits (221), Expect = 2.128e-18
Identity = 41/60 (68.33%), Postives = 54/60 (90.00%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            +I SKMGEGTFGQVLEC+D + KE+VAIK++R I KYREAAMIEI++LQ+L ++D+GG+R
Sbjct:  102 QILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSR 161

[Close]

BLAST of GT623149 vs. TAIR peptide
Match: AT4G32660.3 (| Symbols: AME3 | Protein kinase superfamily protein | chr4:15756420-15759107 FORWARD LENGTH=392)

HSP 1 Score: 226.868 bits (577), Expect = 1.115e-59
Identity = 111/165 (67.27%), Postives = 134/165 (81.21%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRG-KLDWPVGATSRESIKAVTK 838
            MH+L ++HTDLKPENILLVS + VK+PD K S+ +  ++F+ +PKSSAIK+IDFGST  +      IV TRHYR+PEVILGLGWS+ CD+WS+GCIL ELCTGEALFQTH+NLEHLAMMER LGPLP HM +K  R AEKY RRG +L+WP GA SRESI+AV +
Sbjct:  179 MHELQLVHTDLKPENILLVSSENVKLPDNKRSAAN-ETHFRCLPKSSAIKLIDFGSTVCDNRIHHSIVQTRHYRSPEVILGLGWSYQCDLWSIGCILFELCTGEALFQTHDNLEHLAMMERALGPLPEHMTRKASRGAEKYFRRGCRLNWPEGANSRESIRAVKR 342

HSP 2 Score: 90.8929 bits (224), Expect = 9.553e-19
Identity = 44/60 (73.33%), Postives = 52/60 (86.67%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            KI SKMGEGTFG+VLECWDR+ KE VAIKI+R IKKYR+AAMIEI++LQ+L K+D G  R
Sbjct:   64 KILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEIDVLQKLVKSDKGRTR 123

[Close]

BLAST of GT623149 vs. TAIR peptide
Match: AT4G32660.1 (| Symbols: AME3 | Protein kinase superfamily protein | chr4:15756396-15759107 FORWARD LENGTH=400)

HSP 1 Score: 226.868 bits (577), Expect = 1.115e-59
Identity = 111/165 (67.27%), Postives = 134/165 (81.21%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRG-KLDWPVGATSRESIKAVTK 838
            MH+L ++HTDLKPENILLVS + VK+PD K S+ +  ++F+ +PKSSAIK+IDFGST  +      IV TRHYR+PEVILGLGWS+ CD+WS+GCIL ELCTGEALFQTH+NLEHLAMMER LGPLP HM +K  R AEKY RRG +L+WP GA SRESI+AV +
Sbjct:  187 MHELQLVHTDLKPENILLVSSENVKLPDNKRSAAN-ETHFRCLPKSSAIKLIDFGSTVCDNRIHHSIVQTRHYRSPEVILGLGWSYQCDLWSIGCILFELCTGEALFQTHDNLEHLAMMERALGPLPEHMTRKASRGAEKYFRRGCRLNWPEGANSRESIRAVKR 350

HSP 2 Score: 90.8929 bits (224), Expect = 9.553e-19
Identity = 44/60 (73.33%), Postives = 52/60 (86.67%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            KI SKMGEGTFG+VLECWDR+ KE VAIKI+R IKKYR+AAMIEI++LQ+L K+D G  R
Sbjct:   72 KILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEIDVLQKLVKSDKGRTR 131

[Close]

BLAST of GT623149 vs. TAIR peptide
Match: AT4G32660.2 (| Symbols: AME3 | Protein kinase superfamily protein | chr4:15756396-15758881 FORWARD LENGTH=356)

HSP 1 Score: 226.868 bits (577), Expect = 1.115e-59
Identity = 111/165 (67.27%), Postives = 134/165 (81.21%), Query Frame = 2

Query:  347 MHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKKVDRHAEKYVRRG-KLDWPVGATSRESIKAVTK 838
            MH+L ++HTDLKPENILLVS + VK+PD K S+ +  ++F+ +PKSSAIK+IDFGST  +      IV TRHYR+PEVILGLGWS+ CD+WS+GCIL ELCTGEALFQTH+NLEHLAMMER LGPLP HM +K  R AEKY RRG +L+WP GA SRESI+AV +
Sbjct:  187 MHELQLVHTDLKPENILLVSSENVKLPDNKRSAAN-ETHFRCLPKSSAIKLIDFGSTVCDNRIHHSIVQTRHYRSPEVILGLGWSYQCDLWSIGCILFELCTGEALFQTHDNLEHLAMMERALGPLPEHMTRKASRGAEKYFRRGCRLNWPEGANSRESIRAVKR 350

HSP 2 Score: 90.8929 bits (224), Expect = 9.553e-19
Identity = 44/60 (73.33%), Postives = 52/60 (86.67%), Query Frame = 1

Query:  166 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIKKYREAAMIEIEMLQQLGKNDIGGNR 345
            KI SKMGEGTFG+VLECWDR+ KE VAIKI+R IKKYR+AAMIEI++LQ+L K+D G  R
Sbjct:   72 KILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEIDVLQKLVKSDKGRTR 131

[Close]

BLAST of GT623149 vs. TAIR peptide
Match: AT2G40120.1 (| Symbols: | Protein kinase superfamily protein | chr2:16755137-16757258 REVERSE LENGTH=570)

HSP 1 Score: 100.523 bits (249), Expect = 1.206e-21
Identity = 56/135 (41.48%), Postives = 73/135 (54.07%), Query Frame = 2

Query:  341 IVMHDLGMIHTDLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVSTRHYRAPEVILGLGWSHPCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMHMLKK 745
            + +H LG+IH DLKPENIL+                       K  K  A+K+ID GS+ +   +    V +R YRAPEVILGL +    D+WS+GCIL ELC+GE LF        LA +  VLGP+   ML+K
Sbjct:  380 VFLHGLGIIHCDLKPENILI-----------------------KSYKRCAVKIIDLGSSCFRSDNLCLYVQSRSYRAPEVILGLPYDEKIDLWSLGCILAELCSGEVLFPNEAVAMILARIVAVLGPIETEMLEK 491

The following BLAST results are available for this feature:

BLAST of GT623149 vs. TrEMBL
BLAST of GT623149 vs. SwissProt
BLAST of GT623149 vs. TAIR peptide

BLAST of GT623149 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7SHG7_VITVI	9.312e-83	90.36	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9RAS6_RICCO	6.036e-82	89.16	Afc, putative OS=Ricinus communis GN=RCOM_1508280 ...	[more]
C6T8M4_SOYBN	1.030e-81	91.36	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6TMJ6_SOYBN	8.716e-81	87.20	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6T7I2_SOYBN	1.942e-80	89.63	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9IF06_POPTR	3.662e-79	86.75	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9I3I2_POPTR	1.065e-78	86.75	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
Q9M598_MESCR	9.019e-78	85.54	Protein kinase MK5 OS=Mesembryanthemum crystallinu...	[more]
A7XW60_SORBI	9.019e-78	81.93	Lammer-type protein kinase OS=Sorghum bicolor GN=A...	[more]
B7FLL1_MEDTR	2.009e-77	84.76	Putative uncharacterized protein OS=Medicago trunc...	[more]

BLAST of GT623149 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
AFC2_ARATH	1.204e-76	82.93	Serine/threonine-protein kinase AFC2 OS=Arabidopsi...	[more]
AFC1_ARATH	7.556e-71	76.05	Serine/threonine-protein kinase AFC1 OS=Arabidopsi...	[more]
AFC3_ARATH	1.132e-58	67.27	Serine/threonine-protein kinase AFC3 OS=Arabidopsi...	[more]
LKH1_SCHPO	8.706e-43	52.80	Dual specificity protein kinase lkh1 OS=Schizosacc...	[more]
CLK2_HUMAN	2.801e-41	50.60	Dual specificity protein kinase CLK2 OS=Homo sapie...	[more]
CLK2_MOUSE	3.658e-41	50.60	Dual specificity protein kinase CLK2 OS=Mus muscul...	[more]
CLK4_HUMAN	5.282e-40	53.02	Dual specificity protein kinase CLK4 OS=Homo sapie...	[more]
CLK4_MOUSE	6.898e-40	53.02	Dual specificity protein kinase CLK4 OS=Mus muscul...	[more]
DOA_DROME	3.424e-39	53.95	Serine/threonine-protein kinase Doa OS=Drosophila ...	[more]
CLK1_HUMAN	1.699e-38	52.35	Dual specificity protein kinase CLK1 OS=Homo sapie...	[more]

BLAST of GT623149 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT4G24740.1	1.186e-77	82.93	\| Symbols: AFC2, AME1, FC2 \| FUS3-complementing ge...	[more]
AT4G24740.2	1.186e-77	82.93	\| Symbols: AFC2, AME1, FC2 \| FUS3-complementing ge...	[more]
AT3G53570.4	7.447e-72	76.05	\| Symbols: AFC1, AME2, FC1 \| FUS3-complementing ge...	[more]
AT3G53570.2	7.447e-72	76.05	\| Symbols: AFC1, AME2, FC1 \| FUS3-complementing ge...	[more]
AT3G53570.1	7.447e-72	76.05	\| Symbols: AFC1, AME2, FC1 \| FUS3-complementing ge...	[more]
AT3G53570.3	7.447e-72	76.05	\| Symbols: AFC1, AME2, FC1 \| FUS3-complementing ge...	[more]
AT4G32660.3	1.115e-59	67.27	\| Symbols: AME3 \| Protein kinase superfamily prote...	[more]
AT4G32660.1	1.115e-59	67.27	\| Symbols: AME3 \| Protein kinase superfamily prote...	[more]
AT4G32660.2	1.115e-59	67.27	\| Symbols: AME3 \| Protein kinase superfamily prote...	[more]
AT2G40120.1	1.206e-21	41.48	\| Symbols: \| Protein kinase superfamily protein \|...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase, catalytic domain	PROFILE	PS50011	PROTEIN_KINASE_DOM	coord: 1..63 score: 9
IPR000719	Protein kinase, catalytic domain	PROFILE	PS50011	PROTEIN_KINASE_DOM	coord: 1..168 score: 20
IPR011009	Protein kinase-like domain	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 2..57 score: 5.6
IPR011009	Protein kinase-like domain	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 5..156 score: 3.6
IPR017442	Serine/threonine-protein kinase-like domain	PFAM	PF00069	Pkinase	coord: 3..53 score: 1.4
IPR017442	Serine/threonine-protein kinase-like domain	PFAM	PF00069	Pkinase	coord: 5..140 score: 1.7
None	No IPR available	GENE3D	3.30.200.20	no description	coord: 3..54 score: 2.1
None	No IPR available	PANTHER	PTHR11295	CDC2, MAP KINASE-RELATED	coord: 2..52 score: 1
None	No IPR available	PANTHER	PTHR11295:SF30	AFC	coord: 2..52 score: 1
None	No IPR available	PANTHER	PTHR11295	CDC2, MAP KINASE-RELATED	coord: 2..30 score: 2.1
None	No IPR available	PANTHER	PTHR11295:SF30	AFC	coord: 2..30 score: 2.1
None	No IPR available	GENE3D	1.10.510.10	no description	coord: 5..148 score: 5.4
None	No IPR available	PANTHER	PTHR11295	CDC2, MAP KINASE-RELATED	coord: 5..127 score: 3.1
None	No IPR available	PANTHER	PTHR11295:SF30	AFC	coord: 5..127 score: 3.1
IPR002290	Serine/threonine-protein kinase domain	SMART	SM00220	no description	coord: 1..146 score: 0.
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 10..22 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT623149 ID=GT623149; Name=GT623149; organism=Lens culinaris; type=EST; length=839bp
AATCTTCTTCAATTCGATTTTCTTTCATCAAATTTTGCTCTGAACTTTTC
GCACCTTTTGAATCATGGAGATGGAACGCGTCTTCGAATTTCCTCATACT
CACATGGATCGGCGCCCTAGAAAGAGAGCGAGGCTCGGATGGGATGTCCC
TGAGGTTCCTAAGATAAGATCCACAGCAAAATGGGTGAAGGCACTTTTGG
GCAGGTATTGGAATGCTGGGACAGAGAGAAGAAGGAAATGGTTGCCATAA
AGATTGTTCGTGGAATAAAGAAGTATCGGGAAGCAGCCATGATAGAAATT
GAGATGTTGCAACAGCTCGGTAAAAATGATATAGGAGGCAATCGTTATGC
ATGATTTGGGCATGATTCATACTGATCTGAAGCCTGAAAACATACTCCTT
GTTTCACCAGACTATGTCAAGGTTCCTGACTATAAGATTTCATCTAGATC
ACCAAATTCATATTTCAAAAAAGTCCCCAAGTCTAGTGCTATCAAGGTTA
TTGATTTTGGAAGCACAACGTATGAGCGAGTAGATCAAAGTTACATTGTT
TCAACTCGTCATTATAGAGCACCTGAAGTTATCCTTGGTCTTGGATGGAG
TCATCCTTGTGATATATGGAGTGTGGGGTGTATATTGGTTGAGTTGTGCA
CGGGTGAAGCTTTATTCCAGACTCATGAAAATTTAGAGCACCTTGCCATG
ATGGAGAGGGTTCTCGGTCCACTACCAATGCATATGTTGAAGAAAGTAGA
CCGACATGCTGAGAAGTATGTCAGACGAGGCAAATTAGACTGGCCAGTTG
GTGCAACTTCTAGGGAGAGTATTAAAGCTGTAACTAAGC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004672	protein kinase activity
GO:0005524	ATP binding
GO:0004674	protein serine/threonine kinase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006468	protein phosphorylation

Vocabulary: **INTERPRO**
Term	Definition
IPR000719	Prot_kinase_dom
IPR011009	Kinase-like_dom_sf
IPR017442	Serine/threonine-protein kinase-like domain
IPR002290	Ser/Thr_dual-sp_kinase
IPR008271	Ser/Thr_kinase_AS

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