GT624236

EST Overview


Name	GT624236
Unique Name	GT624236
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	589

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
KEGG analysis for Lens culinaris unigene v1	2011-01-05
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT624236 vs. TrEMBL
Match: B7FKV2_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 337.421 bits (864), Expect = 4.372e-91
Identity = 162/166 (97.59%), Postives = 166/166 (100.00%), Query Frame = 2

Query:    2 SVKVTSYEEEALRTMKAFWLPSATPEASVKVDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRSYVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAKDFKHLGSGSGLGLVRPAMKT 499
            SVKVTSYEEEALRTMKAFWLPSATPEASVKV+APST+TICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRSY+CPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLK+GGTGFAGHDDNLEAKDFKHLGSGSGLGLVRPAMKT
Sbjct:  139 SVKVTSYEEEALRTMKAFWLPSATPEASVKVEAPSTSTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRSYICPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKKGGTGFAGHDDNLEAKDFKHLGSGSGLGLVRPAMKT 304

[Close]

BLAST of GT624236 vs. TrEMBL
Match: C6TGM6_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 311.227 bits (796), Expect = 3.355e-83
Identity = 146/166 (87.95%), Postives = 160/166 (96.39%), Query Frame = 2

Query:    2 SVKVTSYEEEALRTMKAFWLPSATPEASVKVDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRSYVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAKDFKHLGSGSGLGLVRPAMKT 499
            S+KVTSYEEEALRTMKAFWLPSATPEAS+KVDAPS +TICPEG E LKLKTLFPVQFTEDT+EQKKS ALD++Y+CPSCK TLTNTMSLVALS+CGHVFCKKC+DRFMAVDKVCLVCNKACKER+LVNL++GGTGFAGH D+LEA+DFKHLGSGSGLGLVRPAMKT
Sbjct:  139 SLKVTSYEEEALRTMKAFWLPSATPEASIKVDAPSNSTICPEGNEKLKLKTLFPVQFTEDTTEQKKSKALDKTYICPSCKVTLTNTMSLVALSTCGHVFCKKCADRFMAVDKVCLVCNKACKERNLVNLEKGGTGFAGHGDHLEARDFKHLGSGSGLGLVRPAMKT 304

[Close]

BLAST of GT624236 vs. TrEMBL
Match: B9HYP8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_568327 PE=4 SV=1)

HSP 1 Score: 293.123 bits (749), Expect = 9.455e-78
Identity = 137/166 (82.53%), Postives = 154/166 (92.77%), Query Frame = 2

Query:    2 SVKVTSYEEEALRTMKAFWLPSATPEASVKVDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRSYVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAKDFKHLGSGSGLGLVRPAMKT 499
            SVKVTSYEEEALRTMKAFWLPSATP A VKVDAPST+T+CPEGKE LKLK+LFPV FTEDTSE+K S++LD++++CPSCK TLTNT+SLVALSSCGHVFCKKC+D+FMAVDKVCL C K CKER+LVNL++GGTGFAGHDD+L A DFKHLGSGSGLGLVRPA KT
Sbjct:  139 SVKVTSYEEEALRTMKAFWLPSATPVAPVKVDAPSTSTVCPEGKEKLKLKSLFPVYFTEDTSEKKSSSSLDKTFICPSCKVTLTNTLSLVALSSCGHVFCKKCADKFMAVDKVCLACGKGCKERNLVNLEKGGTGFAGHDDHLVATDFKHLGSGSGLGLVRPATKT 304

[Close]

BLAST of GT624236 vs. TrEMBL
Match: D7UDQ9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_203.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00003678001 PE=4 SV=1)

HSP 1 Score: 292.352 bits (747), Expect = 1.613e-77
Identity = 137/166 (82.53%), Postives = 152/166 (91.57%), Query Frame = 2

Query:    2 SVKVTSYEEEALRTMKAFWLPSATPEASVKVDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRSYVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAKDFKHLGSGSGLGLVRPAMKT 499
            SVKVTSYEEEALR MKAFWLPSATPEA VKV+APST+T+CPEG E LKLKTLFP+  TED SEQKKS +LD++Y+CPSCK TLTNTMSLVALSSCGHVFCKKC+ +FMAVDKVCLVCNK CK+R+LV L++GGTGFAGH D+LEA DFKHLGSGSGLGLVRPAMKT
Sbjct:  211 SVKVTSYEEEALRNMKAFWLPSATPEAPVKVEAPSTSTVCPEGNEKLKLKTLFPIHLTEDRSEQKKSKSLDKTYICPSCKVTLTNTMSLVALSSCGHVFCKKCAGKFMAVDKVCLVCNKGCKDRNLVPLEKGGTGFAGHGDHLEATDFKHLGSGSGLGLVRPAMKT 376

[Close]

BLAST of GT624236 vs. TrEMBL
Match: B9P5E3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_829094 PE=4 SV=1)

HSP 1 Score: 291.967 bits (746), Expect = 2.106e-77
Identity = 137/166 (82.53%), Postives = 154/166 (92.77%), Query Frame = 2

Query:    2 SVKVTSYEEEALRTMKAFWLPSATPEASVKVDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRSYVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAKDFKHLGSGSGLGLVRPAMKT 499
            SVKVTSYEEEALRTMKAFWLPSATP+A VKVDAPST+T+CPEGKE LKLK+LFPV FTEDTSE+K S++LD++Y+CPSCK TLTNT+SLVALSSCGHVFCKKCSD+FMAVDKVCLVC K CKER+LVNL +GGTGFAGH D+L A +FKHLGSGSGLGLVRPA K+
Sbjct:  139 SVKVTSYEEEALRTMKAFWLPSATPDAPVKVDAPSTSTVCPEGKEKLKLKSLFPVYFTEDTSEKKSSSSLDKTYICPSCKVTLTNTLSLVALSSCGHVFCKKCSDKFMAVDKVCLVCTKGCKERNLVNLGKGGTGFAGHGDHLMATEFKHLGSGSGLGLVRPAAKS 304

[Close]

BLAST of GT624236 vs. TrEMBL
Match: B9H1U1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_800358 PE=4 SV=1)

HSP 1 Score: 291.967 bits (746), Expect = 2.106e-77
Identity = 137/166 (82.53%), Postives = 154/166 (92.77%), Query Frame = 2

Query:    2 SVKVTSYEEEALRTMKAFWLPSATPEASVKVDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRSYVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAKDFKHLGSGSGLGLVRPAMKT 499
            SVKVTSYEEEALRTMKAFWLPSATP+A VKVDAPST+T+CPEGKE LKLK+LFPV FTEDTSE+K S++LD++Y+CPSCK TLTNT+SLVALSSCGHVFCKKCSD+FMAVDKVCLVC K CKER+LVNL +GGTGFAGH D+L A +FKHLGSGSGLGLVRPA K+
Sbjct:  139 SVKVTSYEEEALRTMKAFWLPSATPDAPVKVDAPSTSTVCPEGKEKLKLKSLFPVYFTEDTSEKKSSSSLDKTYICPSCKVTLTNTLSLVALSSCGHVFCKKCSDKFMAVDKVCLVCTKGCKERNLVNLGKGGTGFAGHGDHLMATEFKHLGSGSGLGLVRPAAKS 304

[Close]

BLAST of GT624236 vs. TrEMBL
Match: B9SFE5_RICCO (Ubiquitin-protein ligase, putative OS=Ricinus communis GN=RCOM_1096770 PE=4 SV=1)

HSP 1 Score: 286.574 bits (732), Expect = 8.850e-76
Identity = 133/166 (80.12%), Postives = 151/166 (90.96%), Query Frame = 2

Query:    2 SVKVTSYEEEALRTMKAFWLPSATPEASVKVDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRSYVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAKDFKHLGSGSGLGLVRPAMKT 499
            SVKVTSYEEEALRTMKAFWLPSATPEA  K +AP+T T CPEGKE LK+K+LFPV FTED +E+KKS++LD++Y+CPSCK TLTNT+SLVALSSCGHVFCKKC+D+FM  DKVCLVCNKACKER+LVNL++GGTGFAGH +NL A DFKHLGSGSGLGLVRPA KT
Sbjct:   81 SVKVTSYEEEALRTMKAFWLPSATPEAPAKAEAPTTCTTCPEGKEKLKMKSLFPVYFTEDKNEKKKSSSLDKTYICPSCKVTLTNTLSLVALSSCGHVFCKKCADKFMVADKVCLVCNKACKERNLVNLEKGGTGFAGHGNNLVAMDFKHLGSGSGLGLVRPATKT 246

[Close]

BLAST of GT624236 vs. TrEMBL
Match: Q10N73_ORYSJ (Os03g0279900 protein OS=Oryza sativa subsp. japonica GN=Os03g0279900 PE=4 SV=1)

HSP 1 Score: 271.166 bits (692), Expect = 3.848e-71
Identity = 122/165 (73.94%), Postives = 147/165 (89.09%), Query Frame = 2

Query:    2 SVKVTSYEEEALRTMKAFWLPSATPEASVKVDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRSYVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAKDFKHLGSGSGLGLVRPAMK 496
            SVKVTS+EEEALR MKAFWLPSATPEA+ KV+APST TICPEG+E LKLK+LFP+ FTE+ ++QK   ++++SY+CPSCK+TLTNTMSLV +S+CGHVFCKKCSD+F+  DKVCLVCNK CKER+ V L++GGTGFA HD+ LEA+DFKHLGSGSGLGLV+PA K
Sbjct:  139 SVKVTSFEEEALRNMKAFWLPSATPEATAKVEAPSTDTICPEGQEKLKLKSLFPISFTEENTDQKNKKSVEKSYMCPSCKSTLTNTMSLVTISTCGHVFCKKCSDKFLVTDKVCLVCNKPCKERNFVPLEKGGTGFAAHDERLEARDFKHLGSGSGLGLVKPAPK 303

[Close]

BLAST of GT624236 vs. TrEMBL
Match: B9F7C9_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_10360 PE=4 SV=1)

HSP 1 Score: 271.166 bits (692), Expect = 3.848e-71
Identity = 122/165 (73.94%), Postives = 147/165 (89.09%), Query Frame = 2

Query:    2 SVKVTSYEEEALRTMKAFWLPSATPEASVKVDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRSYVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAKDFKHLGSGSGLGLVRPAMK 496
            SVKVTS+EEEALR MKAFWLPSATPEA+ KV+APST TICPEG+E LKLK+LFP+ FTE+ ++QK   ++++SY+CPSCK+TLTNTMSLV +S+CGHVFCKKCSD+F+  DKVCLVCNK CKER+ V L++GGTGFA HD+ LEA+DFKHLGSGSGLGLV+PA K
Sbjct:  128 SVKVTSFEEEALRNMKAFWLPSATPEATAKVEAPSTDTICPEGQEKLKLKSLFPISFTEENTDQKNKKSVEKSYMCPSCKSTLTNTMSLVTISTCGHVFCKKCSDKFLVTDKVCLVCNKPCKERNFVPLEKGGTGFAAHDERLEARDFKHLGSGSGLGLVKPAPK 292

[Close]

BLAST of GT624236 vs. TrEMBL
Match: B8ALL6_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_11023 PE=4 SV=1)

HSP 1 Score: 271.166 bits (692), Expect = 3.848e-71
Identity = 122/165 (73.94%), Postives = 147/165 (89.09%), Query Frame = 2

Query:    2 SVKVTSYEEEALRTMKAFWLPSATPEASVKVDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRSYVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAKDFKHLGSGSGLGLVRPAMK 496
            SVKVTS+EEEALR MKAFWLPSATPEA+ KV+APST TICPEG+E LKLK+LFP+ FTE+ ++QK   ++++SY+CPSCK+TLTNTMSLV +S+CGHVFCKKCSD+F+  DKVCLVCNK CKER+ V L++GGTGFA HD+ LEA+DFKHLGSGSGLGLV+PA K
Sbjct:  139 SVKVTSFEEEALRNMKAFWLPSATPEATAKVEAPSTDTICPEGQEKLKLKSLFPISFTEENTDQKNKKSVEKSYMCPSCKSTLTNTMSLVTISTCGHVFCKKCSDKFLVTDKVCLVCNKPCKERNFVPLEKGGTGFAAHDERLEARDFKHLGSGSGLGLVKPAPK 303

[Close]

BLAST of GT624236 vs. SwissProt
Match: NOSIP_DICDI (Nitric oxide synthase-interacting protein homolog OS=Dictyostelium discoideum GN=nosip PE=2 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 2.317e-18
Identity = 51/141 (36.17%), Postives = 72/141 (51.06%), Query Frame = 2

Query:   44 MKAFWLPSATPEASVK-VDAPSTTTICP-EGKENLKLKTLFPVQFTEDTSEQKKSNALDRSYVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRF---MAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAKDFKH 451
            + ++W+   TP+   K ++ P + T+CP +GK  LK   L  V FT        SN  +  Y CP C   L+N+     L  CGHVFC  C D+F    +    C VC+K   E  ++ +  GGTGF+G   NLEAK + H
Sbjct:  192 LNSYWV--ITPDNKDKAIEKPRSYTVCPADGKHPLKSAQLINVHFTNVKPSSSDSNDSNNQYCCPICSKVLSNSTKTRLLKRCGHVFC-SCLDKFKEDSSSQLSCYVCDKPYTEDEIIQIHSGGTGFSGSGSNLEAKKYTH 329

[Close]

BLAST of GT624236 vs. SwissProt
Match: NOSIP_DROME (Nitric oxide synthase-interacting protein homolog OS=Drosophila melanogaster GN=CG6179 PE=3 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 2.832e-16
Identity = 44/138 (31.88%), Postives = 76/138 (55.07%), Query Frame = 2

Query:   38 RTMKAFWLPSATPEASV-KVDAPSTTTICPEGKENLKLKTLFPVQFT--EDTSEQKKSNALDRSYVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAKD 442
            + + +FWLPS  P A + K   P  T  CP  ++ L++K L  V+FT  +D   ++   A +  Y+CP     L+N +    L   G V   +C +R +  D +  + ++  KE+ ++ L+RGGTG+A  +D+L+AK+
Sbjct:  163 KKLPSFWLPSECPNAGLAKAQKPDATIYCPVSQKPLRVKDLIDVKFTLLKDGDTKRSLIAKEARYMCPITHDVLSNAVPCAVLRPTGDVVTMECVERLIRKDMIHPLTDRKLKEKDIIPLQRGGTGYATTNDHLQAKE 300

[Close]

BLAST of GT624236 vs. SwissProt
Match: NOSIP_XENLA (Nitric oxide synthase-interacting protein OS=Xenopus laevis GN=nosip PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 6.309e-16
Identity = 47/142 (33.10%), Postives = 71/142 (50.00%), Query Frame = 2

Query:   26 EEALRTMKAFWLPSATPEASVK-VDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRS--YVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAKD 442
            EE  + + +FW+PS TPEA    V  P  T  CP     LK+K L PV FT    +  +   ++R   YVC   +  L N++    L   G V   +C ++ +  D +  +      ER ++ L+RGGTGF+G    L+AK+
Sbjct:  150 EEKNKQLPSFWIPSLTPEAKTSLVKKPDKTVYCPMSGRPLKMKDLMPVNFTRVDEKVDRVGLINRQDRYVCAVTRDMLGNSVPCAVLRPSGAVVTMECVEKLIKKDMIDPISGDKLSERDIIMLQRGGTGFSGSGVLLQAKE 291

[Close]

BLAST of GT624236 vs. SwissProt
Match: NOSIP_XENTR (Nitric oxide synthase-interacting protein OS=Xenopus tropicalis GN=nosip PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.076e-15
Identity = 47/142 (33.10%), Postives = 72/142 (50.70%), Query Frame = 2

Query:   26 EEALRTMKAFWLPSATPEASVK-VDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRS--YVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAKD 442
            EE  + + +FW+PS TPEA    V  P  T  CP   + LK+K L PV FT    +  +   ++R   YVC   +  L N++    L   G V   +C ++ +  D +  +      ER ++ L+RGGTGF+G    L+AK+
Sbjct:  150 EEKGKQLPSFWIPSLTPEAKTSLVKKPDKTVYCPMSGKPLKMKDLIPVHFTAVDEKVDRVGLINRQDRYVCAVTRDMLGNSVPCAVLRPSGAVVTMECVEKLIKKDMIDPISGDKLHERDIIMLQRGGTGFSGSGVLLQAKE 291

[Close]

BLAST of GT624236 vs. SwissProt
Match: NOSIP_MACFA (Nitric oxide synthase-interacting protein OS=Macaca fascicularis GN=NOSIP PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.406e-15
Identity = 47/137 (34.31%), Postives = 72/137 (52.55%), Query Frame = 2

Query:   38 RTMKAFWLPSATPEA-SVKVDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRS--YVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAK 439
            + + +FW+PS TPEA + K++ PS T  CP   + L++  L PV FT   S   +   + RS  YVC   + +L+N      L   G V   +C ++ +  D V  V      +R ++ L+RGGTGFAG    L+A+
Sbjct:  157 KVLPSFWIPSLTPEAKATKLEKPSRTVTCPMSGKPLRMSDLTPVHFTPLDSSVDRVGLITRSERYVCAVTRDSLSNATPCAVLRPSGAVVTLECVEKLIRKDMVDPVTGDKLTDRDIIVLQRGGTGFAGSGVKLQAE 293

[Close]

BLAST of GT624236 vs. SwissProt
Match: NOSIP_HUMAN (Nitric oxide synthase-interacting protein OS=Homo sapiens GN=NOSIP PE=1 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.406e-15
Identity = 47/137 (34.31%), Postives = 72/137 (52.55%), Query Frame = 2

Query:   38 RTMKAFWLPSATPEA-SVKVDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRS--YVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAK 439
            + + +FW+PS TPEA + K++ PS T  CP   + L++  L PV FT   S   +   + RS  YVC   + +L+N      L   G V   +C ++ +  D V  V      +R ++ L+RGGTGFAG    L+A+
Sbjct:  157 KVLPSFWIPSLTPEAKATKLEKPSRTVTCPMSGKPLRMSDLTPVHFTPLDSSVDRVGLITRSERYVCAVTRDSLSNATPCAVLRPSGAVVTLECVEKLIRKDMVDPVTGDKLTDRDIIVLQRGGTGFAGSGVKLQAE 293

[Close]

BLAST of GT624236 vs. SwissProt
Match: NOSIP_DANRE (Nitric oxide synthase-interacting protein OS=Danio rerio GN=nosip PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.836e-15
Identity = 50/150 (33.33%), Postives = 74/150 (49.33%), Query Frame = 2

Query:    2 SVKVTSYEEEALRTMKAFWLPSATPEAS-VKVDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRS--YVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAKD 442
            S   +S E  +   + +FW+PS TPEA    +  PS T  CP     LK+  L  V+FT       +   L R   YVC   K TL N++    L   G V   +C ++ +  D V  +     KE+ ++ ++RGGTGFAG   +L+AK+
Sbjct:  148 STDTSSGESSSSSALPSFWIPSLTPEAKPTLLKKPSKTVSCPMSGRPLKMSDLITVRFTPLDPSLDRVALLTRQDRYVCAVTKDTLGNSVPCAVLRPSGVVVTMECVEKLIKKDMVDPITGDKLKEKDIIPIQRGGTGFAGSGVDLKAKE 297

[Close]

BLAST of GT624236 vs. SwissProt
Match: NOSIP_BOVIN (Nitric oxide synthase-interacting protein OS=Bos taurus GN=NOSIP PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 3.131e-15
Identity = 46/137 (33.58%), Postives = 73/137 (53.28%), Query Frame = 2

Query:   38 RTMKAFWLPSATPEA-SVKVDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRS--YVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAK 439
            + + +FW+PS TPEA + K++ PS    CP   + L++  L PV+FT   S   +   + RS  YVC   + +L+N      L   G V   +C ++ +  D V  V  +   +R ++ L+RGGTGFAG    L+A+
Sbjct:  158 KALPSFWIPSLTPEAKATKLEKPSRIVTCPMSGKPLRMSDLTPVRFTPLDSSVDRVGLITRSERYVCAVTRDSLSNATPCAVLRPSGAVVTLECVEKLIRKDMVDPVTGEKLTDRDIIVLQRGGTGFAGSGVKLQAE 294

[Close]

BLAST of GT624236 vs. SwissProt
Match: NOSIP_MOUSE (Nitric oxide synthase-interacting protein OS=Mus musculus GN=Nosip PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 6.976e-15
Identity = 46/137 (33.58%), Postives = 72/137 (52.55%), Query Frame = 2

Query:   38 RTMKAFWLPSATPEA-SVKVDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRS--YVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAK 439
            + + +FW+PS TPEA + K++ PS T  CP   + L++  L  V+FT+      +   + RS  YVC   + +L+N      L   G V   +C ++ +  D V  V      ER ++ L+RGGTGFAG    L+A+
Sbjct:  157 KALPSFWIPSLTPEAKATKLEKPSRTVTCPMSGKPLRMSDLTSVRFTQLDDSVDRVGLITRSERYVCAVTRDSLSNATPCAVLRPSGAVVTLECVEKLIRKDMVDPVNGDTLTERDIIVLQRGGTGFAGSGVKLQAE 293

[Close]

BLAST of GT624236 vs. SwissProt
Match: NOSIP_CAEEL (Nitric oxide synthase-interacting protein homolog OS=Caenorhabditis elegans GN=R05G6.4 PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 8.824e-10
Identity = 35/129 (27.13%), Postives = 64/129 (49.61%), Query Frame = 2

Query:   41 TMKAFWLPSATPEA-SVKVDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKKSNALDRSYVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDD 424
            ++ +FW+P   P A + K++ PS+  +CP   + +KLK L  V+FT     +    A  R ++CP  +  LTNT     L     V      ++ +  D +  +  +   E  ++ L+RGGTG++  ++
Sbjct:  172 SLPSFWIPELNPTAVATKLEKPSSKVLCPVSGKPIKLKELLEVKFTPMPGTE---TAAHRKFLCPVTRDELTNTTRCAYLKKSKSVVKYDVVEKLIKGDGIDPINGEPMSEDDIIELQRGGTGYSATNE 297

[Close]

BLAST of GT624236 vs. TAIR peptide
Match: AT1G61620.1 (| Symbols: | phosphoinositide binding | chr1:22737653-22739209 FORWARD LENGTH=310)

HSP 1 Score: 268.085 bits (684), Expect = 2.481e-72
Identity = 126/169 (74.56%), Postives = 148/169 (87.57%), Query Frame = 2

Query:    2 SVKVTSYEEEALRTMKAFWLPSATPEASVKVDAPSTTTICPEGKENLKLKTLFPVQFTEDTSEQKK-------SNALDRSYVCPSCKTTLTNTMSLVALSSCGHVFCKKCSDRFMAVDKVCLVCNKACKERHLVNLKRGGTGFAGHDDNLEAKDFKHLGSGSGLGLVRP 487
            SVK TS+EEEALRTMKAFWLPSATP ASV+VDAP T T+CPEGKE LKLK LF ++FTED SE+++       S++ D+SY+CPSCK TLTNTMSLVALSSCGHVFCKKC+++FM VDKVCLVC+K CK+R+LV LK+GGTGFA HDD+LEAK++KHLGSGSGLGLVRP
Sbjct:  139 SVKTTSFEEEALRTMKAFWLPSATPAASVRVDAPETHTVCPEGKEKLKLKNLFAIRFTEDNSEEEETKTKSASSSSYDKSYICPSCKVTLTNTMSLVALSSCGHVFCKKCAEKFMPVDKVCLVCDKPCKDRNLVGLKKGGTGFAEHDDHLEAKEYKHLGSGSGLGLVRP 307

The following BLAST results are available for this feature:

BLAST of GT624236 vs. TrEMBL
BLAST of GT624236 vs. SwissProt
BLAST of GT624236 vs. TAIR peptide

BLAST of GT624236 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FKV2_MEDTR	4.372e-91	97.59	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6TGM6_SOYBN	3.355e-83	87.95	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9HYP8_POPTR	9.455e-78	82.53	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7UDQ9_VITVI	1.613e-77	82.53	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9P5E3_POPTR	2.106e-77	82.53	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9H1U1_POPTR	2.106e-77	82.53	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9SFE5_RICCO	8.850e-76	80.12	Ubiquitin-protein ligase, putative OS=Ricinus comm...	[more]
Q10N73_ORYSJ	3.848e-71	73.94	Os03g0279900 protein OS=Oryza sativa subsp. japoni...	[more]
B9F7C9_ORYSJ	3.848e-71	73.94	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B8ALL6_ORYSI	3.848e-71	73.94	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of GT624236 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
NOSIP_DICDI	2.317e-18	36.17	Nitric oxide synthase-interacting protein homolog ...	[more]
NOSIP_DROME	2.832e-16	31.88	Nitric oxide synthase-interacting protein homolog ...	[more]
NOSIP_XENLA	6.309e-16	33.10	Nitric oxide synthase-interacting protein OS=Xenop...	[more]
NOSIP_XENTR	1.076e-15	33.10	Nitric oxide synthase-interacting protein OS=Xenop...	[more]
NOSIP_MACFA	1.406e-15	34.31	Nitric oxide synthase-interacting protein OS=Macac...	[more]
NOSIP_HUMAN	1.406e-15	34.31	Nitric oxide synthase-interacting protein OS=Homo ...	[more]
NOSIP_DANRE	1.836e-15	33.33	Nitric oxide synthase-interacting protein OS=Danio...	[more]
NOSIP_BOVIN	3.131e-15	33.58	Nitric oxide synthase-interacting protein OS=Bos t...	[more]
NOSIP_MOUSE	6.976e-15	33.58	Nitric oxide synthase-interacting protein OS=Mus m...	[more]
NOSIP_CAEEL	8.824e-10	27.13	Nitric oxide synthase-interacting protein homolog ...	[more]

BLAST of GT624236 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G61620.1	2.481e-72	74.56	\| Symbols: \| phosphoinositide binding \| chr1:2273...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 76..118 score: 10
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	no description	coord: 59..128 score: 1.3
None	No IPR available	PANTHER	PTHR13063	ENOS INTERACTING PROTEIN	coord: 1..156 score: 5.4e
None	No IPR available	PANTHER	PTHR13063:SF3	SUBFAMILY NOT NAMED	coord: 1..156 score: 5.4e
None	No IPR available	PFAM	PF04641	Rtf2	coord: 48..130 score: 1.3
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 71..133 score: 4.9

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT624236 ID=GT624236; Name=GT624236; organism=Lens culinaris; type=EST; length=589bp
CAGTGTGAAGGTCACTTCCTATGAGGAGGAAGCCCTTAGGACAATGAAGG
CATTTTGGCTTCCTTCTGCTACACCAGAAGCTTCTGTTAAAGTAGATGCT
CCATCTACCACTACCATCTGTCCAGAAGGCAAGGAGAATCTGAAGTTAAA
GACACTTTTCCCTGTCCAGTTCACTGAAGACACTAGCGAGCAGAAAAAAT
CCAACGCCCTTGATAGGAGTTACGTTTGCCCTAGCTGCAAAACCACTCTC
ACCAACACAATGTCACTTGTAGCCCTCAGTTCATGTGGGCATGTTTTTTG
CAAAAAATGTTCCGACAGATTTATGGCTGTCGACAAGGTTTGTCTTGTTT
GCAACAAGGCATGTAAAGAGAGACATCTGGTGAATTTGAAGAGGGGAGGT
ACAGGTTTTGCTGGTCACGATGATAATCTTGAAGCCAAAGATTTCAAACA
CTTGGGAAGCGGCTCTGGTTTGGGGCTGGTTAGACCTGCGATGAAAACTT
AAAGCCGTATTCGATTAGAGTGTTGTGGATGATGATTACTTATTTTGCGT
TAAACTTCTCATAATGATGAAATTATGTTGTTATTGCAG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD

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