GT619462

EST Overview


Name	GT619462
Unique Name	GT619462
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	782

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT619462 vs. TrEMBL
Match: Q0WS09_ARATH (Ribosomal protein S27 OS=Arabidopsis thaliana GN=At2g45710 PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 7.081e-10
Identity = 35/58 (60.34%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLLNPPA            VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVMC 58

[Close]

BLAST of GT619462 vs. TrEMBL
Match: D7LS57_ARALY (ARS27A OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_486581 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 7.081e-10
Identity = 35/58 (60.34%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLLNPPA            VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVMC 58

[Close]

BLAST of GT619462 vs. TrEMBL
Match: D7MUU7_ARALY (Ribosomal protein S27 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_917614 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 9.248e-10
Identity = 35/58 (60.34%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLLNPPA            VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVC 58

[Close]

BLAST of GT619462 vs. TrEMBL
Match: D7LD76_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_670538 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 9.248e-10
Identity = 35/58 (60.34%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLLNPPA            VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVC 58

[Close]

BLAST of GT619462 vs. TrEMBL
Match: C6SWV6_SOYBN (Ribosomal protein S27 OS=Glycine max PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 9.248e-10
Identity = 35/58 (60.34%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLLNPPA            VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVC 58

[Close]

BLAST of GT619462 vs. TrEMBL
Match: B9RG16_RICCO (Ribosomal protein S27 OS=Ricinus communis GN=RCOM_1300070 PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 9.248e-10
Identity = 35/58 (60.34%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLLNPPA            VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVC 58

[Close]

BLAST of GT619462 vs. TrEMBL
Match: B7FN54_MEDTR (Ribosomal protein S27 OS=Medicago truncatula PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 9.248e-10
Identity = 35/58 (60.34%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLLNPPA            VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVC 58

[Close]

BLAST of GT619462 vs. TrEMBL
Match: B7FHA3_MEDTR (Ribosomal protein S27 OS=Medicago truncatula PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 9.248e-10
Identity = 35/58 (60.34%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLLNPPA            VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVC 58

[Close]

BLAST of GT619462 vs. TrEMBL
Match: A9PJL7_9ROSI (Putative uncharacterized protein OS=Populus trichocarpa x Populus deltoides PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 9.248e-10
Identity = 35/58 (60.34%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLLNPPA            VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVSSHSQTVVVC 58

[Close]

BLAST of GT619462 vs. TrEMBL
Match: A9P8F6_POPTR (Ribosomal protein S27 OS=Populus trichocarpa GN=POPTRDRAFT_800083 PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 9.248e-10
Identity = 35/58 (60.34%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLLNPPA            VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVC 58

[Close]

BLAST of GT619462 vs. SwissProt
Match: RS271_ARATH (40S ribosomal protein S27-1 OS=Arabidopsis thaliana GN=RPS27A PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 4.602e-11
Identity = 35/58 (60.34%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLLNPPA            VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVMC 58

[Close]

BLAST of GT619462 vs. SwissProt
Match: RS27_HORVU (40S ribosomal protein S27 OS=Hordeum vulgare GN=RPS27 PE=3 SV=2)

HSP 1 Score: 68.1662 bits (165), Expect = 6.010e-11
Identity = 35/58 (60.34%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLLNPPA            VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLQNDIDLLNPPAELEKLKHKKKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVC 58

[Close]

BLAST of GT619462 vs. SwissProt
Match: RS272_ARATH (40S ribosomal protein S27-2 OS=Arabidopsis thaliana GN=RPS27B PE=2 SV=2)

HSP 1 Score: 68.1662 bits (165), Expect = 6.010e-11
Identity = 35/58 (60.34%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLLNPPA            VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVC 58

[Close]

BLAST of GT619462 vs. SwissProt
Match: RS27_CHLRE (40S ribosomal protein S27 OS=Chlamydomonas reinhardtii GN=RPS27 PE=3 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 6.645e-10
Identity = 33/58 (56.90%), Postives = 37/58 (63.79%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVL +DIDLLNPPA            VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLSSDIDLLNPPAELEKTKHKRKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVMC 58

[Close]

BLAST of GT619462 vs. SwissProt
Match: RS273_ARATH (40S ribosomal protein S27-3 OS=Arabidopsis thaliana GN=RPS27D PE=1 SV=2)

HSP 1 Score: 64.3142 bits (155), Expect = 8.679e-10
Identity = 33/58 (56.90%), Postives = 37/58 (63.79%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLL+PP             VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLQNDIDLLHPPPELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVC 58

[Close]

BLAST of GT619462 vs. TAIR peptide
Match: AT2G45710.1 (| Symbols: | Zinc-binding ribosomal protein family protein | chr2:18831243-18831999 FORWARD LENGTH=84)

HSP 1 Score: 68.5514 bits (166), Expect = 4.584e-12
Identity = 35/58 (60.34%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLLNPPA            VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVMC 58

[Close]

BLAST of GT619462 vs. TAIR peptide
Match: AT3G61110.1 (| Symbols: ARS27A, RS27A | ribosomal protein S27 | chr3:22611710-22612632 FORWARD LENGTH=86)

HSP 1 Score: 68.1662 bits (165), Expect = 5.987e-12
Identity = 35/58 (60.34%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLLNPPA            VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVC 58

[Close]

BLAST of GT619462 vs. TAIR peptide
Match: AT5G47930.1 (| Symbols: | Zinc-binding ribosomal protein family protein | chr5:19406423-19407329 REVERSE LENGTH=84)

HSP 1 Score: 64.3142 bits (155), Expect = 8.645e-11
Identity = 33/58 (56.90%), Postives = 37/58 (63.79%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLL+PP             VQSPNSFFMDVKCQGCFN+        TV++C
Sbjct:    1 MVLQNDIDLLHPPPELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVC 58

[Close]

BLAST of GT619462 vs. TAIR peptide
Match: AT3G61111.1 (| Symbols: | Zinc-binding ribosomal protein family protein | chr3:22613230-22613919 REVERSE LENGTH=86)

HSP 1 Score: 52.7582 bits (125), Expect = 2.603e-7
Identity = 28/58 (48.28%), Postives = 33/58 (56.90%), Query Frame = 1

Query:   46 MVLQNDIDLLNPPAXXXXXXXXXXXXVQSPNSFFMDVKCQGCFNM*V-----PTVILC 204
            MVLQNDIDLLNPPA            V SPNS+FM V+C  C N+        TV++C
Sbjct:    1 MVLQNDIDLLNPPAELEKRKHKLKRLVPSPNSYFMLVRCSDCINITTIFSHSQTVVVC 58

The following BLAST results are available for this feature:

BLAST of GT619462 vs. TrEMBL
BLAST of GT619462 vs. SwissProt
BLAST of GT619462 vs. TAIR peptide

BLAST of GT619462 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q0WS09_ARATH	7.081e-10	60.34	Ribosomal protein S27 OS=Arabidopsis thaliana GN=A...	[more]
D7LS57_ARALY	7.081e-10	60.34	ARS27A OS=Arabidopsis lyrata subsp. lyrata GN=ARAL...	[more]
D7MUU7_ARALY	9.248e-10	60.34	Ribosomal protein S27 OS=Arabidopsis lyrata subsp....	[more]
D7LD76_ARALY	9.248e-10	60.34	Predicted protein OS=Arabidopsis lyrata subsp. lyr...	[more]
C6SWV6_SOYBN	9.248e-10	60.34	Ribosomal protein S27 OS=Glycine max PE=3 SV=1	[more]
B9RG16_RICCO	9.248e-10	60.34	Ribosomal protein S27 OS=Ricinus communis GN=RCOM_...	[more]
B7FN54_MEDTR	9.248e-10	60.34	Ribosomal protein S27 OS=Medicago truncatula PE=3 ...	[more]
B7FHA3_MEDTR	9.248e-10	60.34	Ribosomal protein S27 OS=Medicago truncatula PE=3 ...	[more]
A9PJL7_9ROSI	9.248e-10	60.34	Putative uncharacterized protein OS=Populus tricho...	[more]
A9P8F6_POPTR	9.248e-10	60.34	Ribosomal protein S27 OS=Populus trichocarpa GN=PO...	[more]

BLAST of GT619462 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 5


Match Name	E-value	Identity	Description
RS271_ARATH	4.602e-11	60.34	40S ribosomal protein S27-1 OS=Arabidopsis thalian...	[more]
RS27_HORVU	6.010e-11	60.34	40S ribosomal protein S27 OS=Hordeum vulgare GN=RP...	[more]
RS272_ARATH	6.010e-11	60.34	40S ribosomal protein S27-2 OS=Arabidopsis thalian...	[more]
RS27_CHLRE	6.645e-10	56.90	40S ribosomal protein S27 OS=Chlamydomonas reinhar...	[more]
RS273_ARATH	8.679e-10	56.90	40S ribosomal protein S27-3 OS=Arabidopsis thalian...	[more]

BLAST of GT619462 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT2G45710.1	4.584e-12	60.34	\| Symbols: \| Zinc-binding ribosomal protein famil...	[more]
AT3G61110.1	5.987e-12	60.34	\| Symbols: ARS27A, RS27A \| ribosomal protein S27 \|...	[more]
AT5G47930.1	8.645e-11	56.90	\| Symbols: \| Zinc-binding ribosomal protein famil...	[more]
AT3G61111.1	2.603e-7	48.28	\| Symbols: \| Zinc-binding ribosomal protein famil...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000592	Ribosomal protein S27e	PANTHER	PTHR11594	40S RIBOSOMAL PROTEIN S27	coord: 22..60 score: 2.7
IPR000592	Ribosomal protein S27e	PFAM	PF01667	Ribosomal_S27e	coord: 45..59 score: 0.0
None	No IPR available	GENE3D	2.20.25.100	no description	coord: 45..59 score: 0.0
None	No IPR available	SEG	seg	seg	coord: 30..41 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT619462 ID=GT619462; Name=GT619462; organism=Lens culinaris; type=EST; length=782bp
TCCACTCTTTCTAGGTTTACGCATCAAACCGCCGGCGGCGCAAAAATGGT
TCTTCAAAACGATATTGATCTGTTGAATCCACCTGCTGAACTCGAGAAGA
GAAAGCATAAACTTAAACGACTTGTTCAATCTCCCAATTCATTCTTCATG
GATGTCAAATGTCAAGGTTGCTTCAATATGTAAGTTCCTACTGTGATCCT
TTGTTATTTATTCCTACTCAGGCGCATTTGAATTAGAAAATACAGATTTT
AGAAGTGATTTTGATTATGATTATACAATTTTTTAGGTATAAATATGATT
TAAGCCTCTTGAAATTAGAAAATACAGATTTTATAAGTGATTTTGATTAT
GATTATACAATTTTTTAGGTCTAAATATGATTTAAGCCTCTTGAAATTTG
AGTTTTCACTTGTTAGTCCTTGTATCTACTTTATAGTTGTGACAGCTAGG
TTTTTTTAGGGCGAGCTAGGTTTTTTTAGAGCTAAATATGATTTAGGTGG
TATCAATTTTACCATCCCTCTATTAGGGACTATTAAAGTAAGACTATCAA
AGTAAGAGCATAGTTTAGTTTAGTTATTTATAGGGACTGAAAAAACAGCA
TAGTGCTGAGTAAAATTTTTCCTCTTCTTCAAAGCTACTCCTTAGTATTA
TATTGTTGGAGAATTCAACATTCCCCTTTAAAATTGACATTATAACTTCT
AAGTATCTGTTTGGATAAACCGATTATTGCACAAGTGCTTATCAAAACAA
GCAGTTATGTATAAGCTTACATAATCTATTTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003735	structural constituent of ribosome

Vocabulary: **Cellular Component**
Term	Definition
GO:0005622	intracellular anatomical structure
GO:0005840	ribosome

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation

Vocabulary: **INTERPRO**
Term	Definition
IPR000592	Ribosomal_eS27

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