GT623799

EST Overview


Name	GT623799
Unique Name	GT623799
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	709

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
KEGG analysis for Lens culinaris unigene v1	2011-01-05
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT623799 vs. TrEMBL
Match: A2Q516_MEDTR (SAM (And some other nucleotide) binding motif OS=Medicago truncatula GN=MtrDRAFT_AC158497g19v2 PE=4 SV=1)

HSP 1 Score: 247.284 bits (630), Expect = 9.052e-64
Identity = 122/140 (87.14%), Postives = 125/140 (89.29%), Query Frame = -3

Query:  288 DIQGKGFPDEFIGLADSVFLDLPQPWLAIPSAAKMLKQDGTLCSFSPCVEQVQRSCDALQSCFTDIRTFEVLLRKYEVREEKMESLHGDDNGSNGSLSRKRRQCSDGNYVLSNSTSSVMARPCGEARGHTGYLTFARLKC 707
            DIQG+GFPDE   LADSVFLDLPQPWLAIPSAAKMLKQDGT CSFSPC+EQVQRSCDALQSCFTDIRTFEVLLR YEVRE K +S HGDD   NGSL  KRRQCSDGNYVLSN TSSVMARPC EARGHTGYLTFARLKC
Sbjct:  169 DIQGEGFPDELNELADSVFLDLPQPWLAIPSAAKMLKQDGTFCSFSPCIEQVQRSCDALQSCFTDIRTFEVLLRTYEVREVKTKSFHGDD---NGSLPCKRRQCSDGNYVLSNPTSSVMARPCDEARGHTGYLTFARLKC 305

[Close]

BLAST of GT623799 vs. TrEMBL
Match: B9S623_RICCO (tRNA, putative OS=Ricinus communis GN=RCOM_1063210 PE=4 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 6.086e-52
Identity = 104/143 (72.73%), Postives = 118/143 (82.52%), Query Frame = -3

Query:  285 DIQGKGFPDEFIGLADSVFLDLPQPWLAIPSAAKMLKQDGTLCSFSPCVEQVQRSCDALQSCFTDIRTFEVLLRKYEVREEKMESLHGDDNGSNGSLSRKRRQCSDGNYVLSNSTSS--VMARPCGEARGHTGYLTFARLKCV 707
            DIQG+GFPDE+ GL DSVFLDLPQPWLAIPSAAKMLKQDG LCSFSPC+EQVQRSC+ L+S FTDIRTFEVLLR YEVRE KM+   G++  S GS   KRRQ S     + NS+SS  +MARPCG++RGHTGYLTFARLKC+
Sbjct:  168 DIQGEGFPDEYSGLVDSVFLDLPQPWLAIPSAAKMLKQDGVLCSFSPCIEQVQRSCETLRSNFTDIRTFEVLLRTYEVREVKMDCGQGEEGISLGSPPYKRRQRSSEGSNVQNSSSSPVIMARPCGDSRGHTGYLTFARLKCL 310

[Close]

BLAST of GT623799 vs. TrEMBL
Match: B9IMM5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_835969 PE=4 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 5.696e-50
Identity = 100/143 (69.93%), Postives = 117/143 (81.82%), Query Frame = -3

Query:  285 DIQGKGFPDEFIGLADSVFLDLPQPWLAIPSAAKMLKQDGTLCSFSPCVEQVQRSCDALQSCFTDIRTFEVLLRKYEVREEKMESLHGDDNGSNGSLSRKRRQCSDGNYVLSNSTSS--VMARPCGEARGHTGYLTFARLKCV 707
            DIQG+GFPDE+ GLADSVFLDLPQPWLAI SA KMLKQDGTLCSFSPC+EQVQR+C+AL+S F DIRTFEVLLR +EV E KM+S  GD+  S GS   KRRQ S    ++ +S+SS  + ARPC +ARGHTGYLTF+RLKC+
Sbjct:  168 DIQGEGFPDEYSGLADSVFLDLPQPWLAILSAGKMLKQDGTLCSFSPCIEQVQRTCEALKSNFRDIRTFEVLLRTFEVHEGKMDSCQGDEGVSVGSPPYKRRQRSSEGSIVQDSSSSPTIKARPCADARGHTGYLTFSRLKCL 310

[Close]

BLAST of GT623799 vs. TrEMBL
Match: Q84K50_ARATH (Putative uncharacterized protein At5g14600 OS=Arabidopsis thaliana GN=At5g14600 PE=2 SV=1)

HSP 1 Score: 187.963 bits (476), Expect = 6.517e-46
Identity = 97/152 (63.82%), Postives = 117/152 (76.97%), Query Frame = -3

Query:  285 DIQGKGFPDEFIGLADSVFLDLPQPWLAIPSAAKMLKQDGTLCSFSPCVEQVQRSCDALQSCFTDIRTFEVLLRKYEVREEKMES------LHGDDNGSNGSLSRKRRQCSDGNYVL-----SNSTSSVMARPCGEARGHTGYLTFARLKCV 707
            DIQG+GFP++  GLADSVFLDLPQPWLA+PSAAKMLKQDG LCSFSPC+EQVQR+C+ L+S F +IRTFEVLLR YEV+E KM++       H +DN   G    KR+  S+ N  +     SN++S VMARPC EARGHTGYLTFARL+C+
Sbjct:  168 DIQGEGFPEKLSGLADSVFLDLPQPWLAVPSAAKMLKQDGVLCSFSPCIEQVQRTCEVLRSDFIEIRTFEVLLRTYEVKEVKMDTSSMVGESHEEDN--EGMRPCKRKHRSNENDTVSQDNSSNASSVVMARPCSEARGHTGYLTFARLQCL 317

[Close]

BLAST of GT623799 vs. TrEMBL
Match: A5B859_VITVI (Putative uncharacterized protein (Fragment) OS=Vitis vinifera GN=VITISV_033584 PE=4 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 8.512e-46
Identity = 98/144 (68.06%), Postives = 111/144 (77.08%), Query Frame = -3

Query:  282 DIQGKGFPDEFIGLADSVFLDLPQPWLAIPSAAKMLKQDGTLCSFSPCVEQVQRSCDALQSCFTDIRTFEVLLRKYEVREEKMESLHGDDNGSNGSLSRKRRQCSD--GNYVLSNSTSSVMARPCGEARGHTGYLTFARLKCVL 707
            DIQG+GFPDEF GLADSVFLDLPQPWLAIPSA KMLK+DG LCSFSPC+EQVQRS + L S FTDIRTFEVLLR YEVRE KM+   G++ G  G    KRRQ S    N     ++++VMARP  EARGHTG+LTFARL  V+
Sbjct:  235 DIQGEGFPDEFSGLADSVFLDLPQPWLAIPSAGKMLKEDGVLCSFSPCIEQVQRSSETLTSNFTDIRTFEVLLRTYEVREGKMDCSQGNEGGFLGPRPGKRRQRSGEASNEGEIPTSTTVMARPAAEARGHTGFLTFARLNEVV 378

[Close]

BLAST of GT623799 vs. TrEMBL
Match: Q7XTH1_ORYSJ (OSJNBb0026L04.5 protein OS=Oryza sativa subsp. japonica GN=OSJNBb0026L04.5 PE=2 SV=2)

HSP 1 Score: 182.185 bits (461), Expect = 3.576e-44
Identity = 88/141 (62.41%), Postives = 109/141 (77.30%), Query Frame = -3

Query:  291 DIQGKGFPDEFIGLADSVFLDLPQPWLAIPSAAKMLKQDGTLCSFSPCVEQVQRSCDALQSCFTDIRTFEVLLRKYEVREEKMESLHGDDNGSNGSLSRKRRQCSDGNYVL--SNSTSSVMARPCGEARGHTGYLTFARLK 707
            DIQG+GFP+E  G AD+VFLDLPQPWLAIPSA  MLKQDG LCSFSPC+EQVQR+C+A++SCFTDIRTFE+LLR YEVRE  ++    ++  +   L++K+R+       L    ++SSVM RPC  ARGHTGYLTFARL+
Sbjct:  183 DIQGQGFPEEHTGAADAVFLDLPQPWLAIPSAGTMLKQDGVLCSFSPCIEQVQRACEAMRSCFTDIRTFEILLRTYEVREGGLKGATTNEESNAVPLAQKKRKLLAAAETLDVKQNSSSVMVRPCSTARGHTGYLTFARLR 323

[Close]

BLAST of GT623799 vs. TrEMBL
Match: Q0JE69_ORYSJ (Os04g0319600 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os04g0319600 PE=4 SV=2)

HSP 1 Score: 182.185 bits (461), Expect = 3.576e-44
Identity = 88/141 (62.41%), Postives = 109/141 (77.30%), Query Frame = -3

Query:  291 DIQGKGFPDEFIGLADSVFLDLPQPWLAIPSAAKMLKQDGTLCSFSPCVEQVQRSCDALQSCFTDIRTFEVLLRKYEVREEKMESLHGDDNGSNGSLSRKRRQCSDGNYVL--SNSTSSVMARPCGEARGHTGYLTFARLK 707
            DIQG+GFP+E  G AD+VFLDLPQPWLAIPSA  MLKQDG LCSFSPC+EQVQR+C+A++SCFTDIRTFE+LLR YEVRE  ++    ++  +   L++K+R+       L    ++SSVM RPC  ARGHTGYLTFARL+
Sbjct:   57 DIQGQGFPEEHTGAADAVFLDLPQPWLAIPSAGTMLKQDGVLCSFSPCIEQVQRACEAMRSCFTDIRTFEILLRTYEVREGGLKGATTNEESNAVPLAQKKRKLLAAAETLDVKQNSSSVMVRPCSTARGHTGYLTFARLR 197

[Close]

BLAST of GT623799 vs. TrEMBL
Match: Q01M41_ORYSA (H0725E11.5 protein OS=Oryza sativa GN=H0725E11.5 PE=4 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 3.576e-44
Identity = 88/141 (62.41%), Postives = 109/141 (77.30%), Query Frame = -3

Query:  291 DIQGKGFPDEFIGLADSVFLDLPQPWLAIPSAAKMLKQDGTLCSFSPCVEQVQRSCDALQSCFTDIRTFEVLLRKYEVREEKMESLHGDDNGSNGSLSRKRRQCSDGNYVL--SNSTSSVMARPCGEARGHTGYLTFARLK 707
            DIQG+GFP+E  G AD+VFLDLPQPWLAIPSA  MLKQDG LCSFSPC+EQVQR+C+A++SCFTDIRTFE+LLR YEVRE  ++    ++  +   L++K+R+       L    ++SSVM RPC  ARGHTGYLTFARL+
Sbjct:  183 DIQGQGFPEEHTGAADAVFLDLPQPWLAIPSAGTMLKQDGVLCSFSPCIEQVQRACEAMRSCFTDIRTFEILLRTYEVREGGLKGATTNEESNAVPLAQKKRKLLAAAETLDVKQNSSSVMVRPCSTARGHTGYLTFARLR 323

[Close]

BLAST of GT623799 vs. TrEMBL
Match: A3ASF2_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_14292 PE=4 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 3.576e-44
Identity = 88/141 (62.41%), Postives = 109/141 (77.30%), Query Frame = -3

Query:  291 DIQGKGFPDEFIGLADSVFLDLPQPWLAIPSAAKMLKQDGTLCSFSPCVEQVQRSCDALQSCFTDIRTFEVLLRKYEVREEKMESLHGDDNGSNGSLSRKRRQCSDGNYVL--SNSTSSVMARPCGEARGHTGYLTFARLK 707
            DIQG+GFP+E  G AD+VFLDLPQPWLAIPSA  MLKQDG LCSFSPC+EQVQR+C+A++SCFTDIRTFE+LLR YEVRE  ++    ++  +   L++K+R+       L    ++SSVM RPC  ARGHTGYLTFARL+
Sbjct:  199 DIQGQGFPEEHTGAADAVFLDLPQPWLAIPSAGTMLKQDGVLCSFSPCIEQVQRACEAMRSCFTDIRTFEILLRTYEVREGGLKGATTNEESNAVPLAQKKRKLLAAAETLDVKQNSSSVMVRPCSTARGHTGYLTFARLR 339

[Close]

BLAST of GT623799 vs. TrEMBL
Match: A2WPJ2_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_01770 PE=4 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 3.576e-44
Identity = 88/141 (62.41%), Postives = 109/141 (77.30%), Query Frame = -3

Query:  291 DIQGKGFPDEFIGLADSVFLDLPQPWLAIPSAAKMLKQDGTLCSFSPCVEQVQRSCDALQSCFTDIRTFEVLLRKYEVREEKMESLHGDDNGSNGSLSRKRRQCSDGNYVL--SNSTSSVMARPCGEARGHTGYLTFARLK 707
            DIQG+GFP+E  G AD+VFLDLPQPWLAIPSA  MLKQDG LCSFSPC+EQVQR+C+A++SCFTDIRTFE+LLR YEVRE  ++    ++  +   L++K+R+       L    ++SSVM RPC  ARGHTGYLTFARL+
Sbjct:  183 DIQGQGFPEEHTGAADAVFLDLPQPWLAIPSAGTMLKQDGVLCSFSPCIEQVQRACEAMRSCFTDIRTFEILLRTYEVREGGLKGATTNEESNAVPLAQKKRKLLAAAETLDVKQNSSSVMVRPCSTARGHTGYLTFARLR 323

[Close]

BLAST of GT623799 vs. SwissProt
Match: TRM61_DICDI (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit trmt61a OS=Dictyostelium discoideum GN=trmt61a PE=1 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.196e-20
Identity = 54/131 (41.22%), Postives = 79/131 (60.31%), Query Frame = -3

Query:  297 DSVFLDLPQPWLAIPSAAKMLKQDGTLCSFSPCVEQVQRSCDAL-QSCFTDIRTFEVLLRKYEVREEKMESL--------HGDDNGSNGSLSRKRRQCSDGNYVLSNSTSSVMARPCGEARGHTGYLTFAR 662
            D+VFLDLP PW AI +A  ++     LCSFSPC+EQVQ +C  L  S F +I+T EVL+R ++ R ++ E L        + ++N +N ++   R +   G  +       ++++P  EARGHTGYLTFAR
Sbjct:  178 DAVFLDLPSPWDAIENAIAVMHDGSMLCSFSPCIEQVQNTCLKLADSKFQEIKTIEVLIRTFDTRLQEYEELNLTNPYIDNNNNNNNNNNIEENRGKFEIGG-IEGLKKDKLLSKPFTEARGHTGYLTFAR 307

[Close]

BLAST of GT623799 vs. SwissProt
Match: TRM61_RAT (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRMT61A OS=Rattus norvegicus GN=Trmt61a PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 3.049e-16
Identity = 55/129 (42.64%), Postives = 70/129 (54.26%), Query Frame = -3

Query:  300 IGLADSVFLDLPQPWLAIPSAAKMLK-QDGTLCSFSPCVEQVQRSCDALQS-CFTDIRTFEVLLRKYEVREEK--MESLHGDDNGSNGSLSRKRRQCSDGNYVLSNSTSSVMARPCGEARGHTGYLTFA 674
            + +AD+VFLD+P PW A+  A   LK + G  CSFSPC+EQVQR+C AL +  FT++ T EVL + Y VR     +  L  DD   N               V S++T      P  E  GHTGYLTFA
Sbjct:  172 VHVADAVFLDIPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNVRTVSLPLPDLGADDLEGN---------------VGSDATPFRSGTPMKETVGHTGYLTFA 285

[Close]

BLAST of GT623799 vs. SwissProt
Match: TRM61_MOUSE (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRMT61A OS=Mus musculus GN=Trmt61a PE=2 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 6.791e-16
Identity = 56/127 (44.09%), Postives = 73/127 (57.48%), Query Frame = -3

Query:  300 IGLADSVFLDLPQPWLAIPSAAKMLK-QDGTLCSFSPCVEQVQRSCDALQS-CFTDIRTFEVLLRKYEVREEKMESLHGDDNGSNGSLSRKRRQCSDGNYVLSNSTSSVMARPCGEARGHTGYLTFA 674
            + +AD+VFLD+P PW A+  A   LK + G  CSFSPC+EQVQR+C AL +  FT++ T EVL + Y VR     SL   D G+N   + +    SD +   S +       P  E  GHTGYLTFA
Sbjct:  172 VHVADAVFLDIPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNVR---TVSLPLPDLGAN---NLETNMGSDASPFRSGT-------PMKETVGHTGYLTFA 285

[Close]

BLAST of GT623799 vs. SwissProt
Match: TRM61_HUMAN (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRMT61A OS=Homo sapiens GN=TRMT61A PE=1 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 3.371e-15
Identity = 55/125 (44.00%), Postives = 72/125 (57.60%), Query Frame = -3

Query:  300 LADSVFLDLPQPWLAIPSAAKMLK-QDGTLCSFSPCVEQVQRSCDALQS-CFTDIRTFEVLLRKYEVREEKMESLHGDDNGSNGSLSRKRRQCSDGNYVLSNSTSSVMARPCGEARGHTGYLTFA 668
            +AD+VFLD+P PW A+  A   LK + G  CSFSPC+EQVQR+C AL +  F+++ T EVL + Y VR     SL   D G+           +DG    S+++      P  EA GHTGYLTFA
Sbjct:  174 VADAVFLDIPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAARGFSELSTLEVLPQVYNVR---TVSLPPPDLGTG----------TDGP-AGSDTSPFRSGTPMKEAVGHTGYLTFA 284

[Close]

BLAST of GT623799 vs. SwissProt
Match: TRM61_BOVIN (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRMT61A OS=Bos taurus GN=TRMT61A PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 3.371e-15
Identity = 55/125 (44.00%), Postives = 69/125 (55.20%), Query Frame = -3

Query:  300 LADSVFLDLPQPWLAIPSAAKMLK-QDGTLCSFSPCVEQVQRSCDALQSC-FTDIRTFEVLLRKYEVREEKMESLHGDDNGSNGSLSRKRRQCSDGNYVLSNSTSSVMARPCGEARGHTGYLTFA 668
            +AD+VFLD+P PW A+  A   LK + G  CSFSPC+EQVQR+C AL +C F+++ T EVL + Y VR     SL   D G+        R   D     S +       P  E  GHTGYLTFA
Sbjct:  174 VADAVFLDIPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAACGFSELSTLEVLPQVYNVRTV---SLPVPDLGA--------RPGPDAAPFRSGT-------PMKETVGHTGYLTFA 280

[Close]

BLAST of GT623799 vs. SwissProt
Match: TRM61_CRYNE (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRM61 OS=Cryptococcus neoformans GN=TRM61 PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.261e-11
Identity = 62/188 (32.98%), Postives = 83/188 (44.15%), Query Frame = -3

Query:  294 DSVFLDLPQPWLAIPSAAKMLKQD--GTLCSFSPCVEQVQRSCDALQS-CFTDIRTFEVLLRKYEV--------------------------REE--------------KMESLHGDD----NGSNGSLSRKRRQCS---DGNYVLSNST---------------SSVMARPCGEARGHTGYLTFARL 662
            + VFLDLP PW AIP A K L++D    +C FSPC+EQV ++   L+S  F+DI T EVL+R +E+                          +EE              K++ +  DD     G  GS  RK  Q S     N   +NS                S+V+ +P  E +GHT YLTFA L
Sbjct:  180 EGVFLDLPAPWEAIPHAVKALRRDIITRICCFSPCLEQVLKTVTCLRSEGFSDISTQEVLIRTHELVTPPPNTTYLSSISSVVSYLREHEQRKEERRLLQIKTAKENNRKVKGIEADDAIPVEGETGS-KRKLEQPSVTGSDNAAPANSRPKTNLLWTEPPNTLPSTVLTKPSPEMKGHTSYLTFAIL 366

[Close]

BLAST of GT623799 vs. SwissProt
Match: TRM61_KLULA (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRM61 OS=Kluyveromyces lactis GN=TRM61 PE=3 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 5.569e-10
Identity = 31/75 (41.33%), Postives = 49/75 (65.33%), Query Frame = -3

Query:  450 ADSVFLDLPQPWLAIPSAAKMLKQDG--TLCSFSPCVEQVQRSCDAL-QSCFTDIRTFEVLLRKYEVREEKMESL 665
            AD +FLDLP PW AIP+   ++ ++    LC FSPC+EQV ++ +AL +  + D++T E+  R+YE R + +  L
Sbjct:  197 ADCIFLDLPAPWDAIPNLTSVISKEKKVNLCCFSPCIEQVDKTLEALEEEGWQDVQTVEIQGRQYEARRQMVRRL 271

[Close]

BLAST of GT623799 vs. SwissProt
Match: TRM61_CANAL (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRM61 OS=Candida albicans GN=TRM61 PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 7.273e-10
Identity = 48/157 (30.57%), Postives = 66/157 (42.04%), Query Frame = -3

Query:  300 DSVFLDLPQPWLAIPSAAKMLKQDGT--LCSFSPCVEQVQRSCDAL-QSCFTDIRTFEVLLRKYEVREEKMES---------------------------------LHGDDNGSNGSLSRKRRQCSDGNYVLSNSTSSVMARPCGEARGHTGYLTFA 662
            D VFLDLP PW AIP    ++       +C FSPC+EQV R+  AL ++ +T+I   EV  +++  R+E + S                                 L   DNG        + +  D NY   N+T S       E + HT YLTFA
Sbjct:  178 DVVFLDLPSPWDAIPHLDSVISTSKAAGICCFSPCIEQVDRTVRALEENGWTEIEIVEVAAKRWSARKEMVRSVADAVQRIREIQNGRKTGLEVMKKGPSEEPPAKLQKTDNGYKTPKKSTKVKEGDENYTWLNATKSE-----SEIKSHTSYLTFA 329

[Close]

BLAST of GT623799 vs. SwissProt
Match: TRM61_ASHGO (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRM61 OS=Ashbya gossypii GN=TRM61 PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 9.499e-10
Identity = 33/75 (44.00%), Postives = 48/75 (64.00%), Query Frame = -3

Query:  450 ADSVFLDLPQPWLAIPSAAKMLKQDGT--LCSFSPCVEQVQRSCDALQ-SCFTDIRTFEVLLRKYEVREEKMESL 665
            AD +FLDLP PW AIP    ++ +D T  LC FSPC+EQV ++ +AL+   + +I T E+  R+YE R + +  L
Sbjct:  196 ADCIFLDLPAPWEAIPHLDAVIAKDRTARLCCFSPCIEQVDKTLEALELHGWEEIETVEIQGRQYESRRQMVRQL 270

[Close]

BLAST of GT623799 vs. SwissProt
Match: TRM61_YEAST (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRM61 OS=Saccharomyces cerevisiae GN=GCD14 PE=1 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 2.116e-9
Identity = 33/76 (43.42%), Postives = 47/76 (61.84%), Query Frame = -3

Query:  447 ADSVFLDLPQPWLAIPSAAKMLKQDGT--LCSFSPCVEQVQRSCDALQSC-FTDIRTFEVLLRKYEVREEKMESLH 665
            A+ VFLDLP PW AIP    ++  D    LC FSPC+EQV ++ D L+   +TD+   E+  R+YE R + + SL+
Sbjct:  197 ANVVFLDLPAPWDAIPHLDSVISVDEKVGLCCFSPCIEQVDKTLDVLEKYGWTDVEMVEIQGRQYESRRQMVRSLN 272

[Close]

BLAST of GT623799 vs. TAIR peptide
Match: AT5G14600.1 (| Symbols: | S-adenosyl-L-methionine-dependent methyltransferases superfamily protein | chr5:4707518-4709022 FORWARD LENGTH=318)

HSP 1 Score: 187.963 bits (476), Expect = 4.430e-48
Identity = 97/152 (63.82%), Postives = 117/152 (76.97%), Query Frame = -3

Query:  285 DIQGKGFPDEFIGLADSVFLDLPQPWLAIPSAAKMLKQDGTLCSFSPCVEQVQRSCDALQSCFTDIRTFEVLLRKYEVREEKMES------LHGDDNGSNGSLSRKRRQCSDGNYVL-----SNSTSSVMARPCGEARGHTGYLTFARLKCV 707
            DIQG+GFP++  GLADSVFLDLPQPWLA+PSAAKMLKQDG LCSFSPC+EQVQR+C+ L+S F +IRTFEVLLR YEV+E KM++       H +DN   G    KR+  S+ N  +     SN++S VMARPC EARGHTGYLTFARL+C+
Sbjct:  168 DIQGEGFPEKLSGLADSVFLDLPQPWLAVPSAAKMLKQDGVLCSFSPCIEQVQRTCEVLRSDFIEIRTFEVLLRTYEVKEVKMDTSSMVGESHEEDN--EGMRPCKRKHRSNENDTVSQDNSSNASSVVMARPCSEARGHTGYLTFARLQCL 317

The following BLAST results are available for this feature:

BLAST of GT623799 vs. TrEMBL
BLAST of GT623799 vs. SwissProt
BLAST of GT623799 vs. TAIR peptide

BLAST of GT623799 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A2Q516_MEDTR	9.052e-64	87.14	SAM (And some other nucleotide) binding motif OS=M...	[more]
B9S623_RICCO	6.086e-52	72.73	tRNA, putative OS=Ricinus communis GN=RCOM_1063210...	[more]
B9IMM5_POPTR	5.696e-50	69.93	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q84K50_ARATH	6.517e-46	63.82	Putative uncharacterized protein At5g14600 OS=Arab...	[more]
A5B859_VITVI	8.512e-46	68.06	Putative uncharacterized protein (Fragment) OS=Vit...	[more]
Q7XTH1_ORYSJ	3.576e-44	62.41	OSJNBb0026L04.5 protein OS=Oryza sativa subsp. jap...	[more]
Q0JE69_ORYSJ	3.576e-44	62.41	Os04g0319600 protein (Fragment) OS=Oryza sativa su...	[more]
Q01M41_ORYSA	3.576e-44	62.41	H0725E11.5 protein OS=Oryza sativa GN=H0725E11.5 P...	[more]
A3ASF2_ORYSJ	3.576e-44	62.41	Putative uncharacterized protein OS=Oryza sativa s...	[more]
A2WPJ2_ORYSI	3.576e-44	62.41	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of GT623799 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
TRM61_DICDI	1.196e-20	41.22	tRNA (adenine-N(1)-)-methyltransferase catalytic s...	[more]
TRM61_RAT	3.049e-16	42.64	tRNA (adenine-N(1)-)-methyltransferase catalytic s...	[more]
TRM61_MOUSE	6.791e-16	44.09	tRNA (adenine-N(1)-)-methyltransferase catalytic s...	[more]
TRM61_HUMAN	3.371e-15	44.00	tRNA (adenine-N(1)-)-methyltransferase catalytic s...	[more]
TRM61_BOVIN	3.371e-15	44.00	tRNA (adenine-N(1)-)-methyltransferase catalytic s...	[more]
TRM61_CRYNE	2.261e-11	32.98	tRNA (adenine-N(1)-)-methyltransferase catalytic s...	[more]
TRM61_KLULA	5.569e-10	41.33	tRNA (adenine-N(1)-)-methyltransferase catalytic s...	[more]
TRM61_CANAL	7.273e-10	30.57	tRNA (adenine-N(1)-)-methyltransferase catalytic s...	[more]
TRM61_ASHGO	9.499e-10	44.00	tRNA (adenine-N(1)-)-methyltransferase catalytic s...	[more]
TRM61_YEAST	2.116e-9	43.42	tRNA (adenine-N(1)-)-methyltransferase catalytic s...	[more]

BLAST of GT623799 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT5G14600.1	4.430e-48	63.82	\| Symbols: \| S-adenosyl-L-methionine-dependent me...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 48..60 score
None	No IPR available	GENE3D	3.40.50.150	no description	coord: 120..138 score: 7.2
None	No IPR available	PANTHER	PTHR12133	UNCHARACTERIZED METHYLTRANSFERASE	coord: 1..84 score: 2.2
None	No IPR available	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 4..139 score: 4.6
IPR014816	tRNA methyltransferase complex GCD14 subunit	PFAM	PF08704	GCD14	coord: 1..137 score: 6.7

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT623799 ID=GT623799; Name=GT623799; organism=Lens culinaris; type=EST; length=709bp
AGTGTAAACAAAGTATGGACCTTGATGGTGAGAATACATGAAAAATGAAT
AAAATTAATGGTGGAATGTCACATATTTTCCCACTGTTTAGTACTTTCTC
AGGCATGTATGTAGCCATGCACATAAGATATACAAAAAATTCCTTTCAAA
ACCTATTATTTTAAGTTTATAACAAACAACAACAATTTCTTGTATCATGC
TCAAATCGAAAAGAAGTACAAAGAACACAATAGTTTTTCTCTGCTACCAA
AATTGAAGGACATGATTGAAAAACCTGGTCATAAAACGCATTTAAGTCTT
GCAAACGTCAAATAGCCTGTGTGACCTCGAGCTTCACCACAAGGTCTAGC
CATGACAGAAGAAGTAGAATTGCTGAGAACATAGTTTCCATCTGAACATT
GTCTCCTCTTGCGAGAAAGAGAACCATTAGAACCATTGTCATCTCCATGC
AAGCTTTCCATTTTCTCTTCTCGAACTTCATATTTGCGTAAGAGTACTTC
AAATGTCCTTATATCTGTGAAGCAGGACTGAAGTGCATCACACGATCGTT
GTACTTGTTCAACACACGGAGAGAATGAGCACAATGTACCATCTTGTTTA
AGCATTTTTGCAGCTGAAGGAATGGCCAACCAAGGTTGAGGCAGATCCAA
AAATACAGAGTCAGCCAAGCCAATAAATTCATCAGGAAATCCCTTACCCT
GAATGTCCC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016429	obsolete tRNA (adenine-N1)-methyltransferase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0030488	tRNA methylation

Vocabulary: **INTERPRO**
Term	Definition
IPR014816	tRNA_MeTrfase_Gcd14

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