GT626222

EST Overview


Name	GT626222
Unique Name	GT626222
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	787

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
KEGG analysis for Lens culinaris unigene v1	2011-01-05
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT626222 vs. TrEMBL
Match: Q43675_VICFA (Putative uncharacterized protein USP OS=Vicia faba GN=USP PE=3 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 1.213e-49
Identity = 95/108 (87.96%), Postives = 101/108 (93.52%), Query Frame = 2

Query:  464 MEFAHLTVLSLFCLAFVGITATSSGEDYWQSVWPNTPLPKTFYDLLIPSGKTNSLPIKPEELNQYSTLFFKHDLHPGKIFTLGNTHSVGNTIRPFTKSKQGVTDSIWL 787
            MEFAHLTVLSLFCLAFVGITATSSGEDYWQS+WPNTPLPKTF DLLIPSG TNSLPIK EEL QYSTLFF+HDLHPGK F LG+T+SVG+ IRPFTKS+QGVTDSIWL
Sbjct:    1 MEFAHLTVLSLFCLAFVGITATSSGEDYWQSIWPNTPLPKTFSDLLIPSGITNSLPIKSEELKQYSTLFFEHDLHPGKNFNLGHTNSVGSIIRPFTKSRQGVTDSIWL 108

[Close]

BLAST of GT626222 vs. TrEMBL
Match: Q45NP1_MEDSA (Embryonic abundant protein-like protein (Fragment) OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 2.293e-40
Identity = 82/109 (75.23%), Postives = 91/109 (83.49%), Query Frame = 2

Query:  464 MEFAHLTVLSLFCLAFVGITAT-SSGEDYWQSVWPNTPLPKTFYDLLIPSGKTNSLPIKPEELNQYSTLFFKHDLHPGKIFTLGNTHSVGNTIRPFTKSKQGVTDSIWL 787
            MEFAHL+VL+LFCLAFV I A+ SS EDYWQSVWPNTPLPK F+DLL+P G+TNSLPIK EELNQYSTLFF+HDL+PGK   LGN  S    +RPFTK  QGVTDSIWL
Sbjct:    1 MEFAHLSVLALFCLAFVRIGASPSSAEDYWQSVWPNTPLPKAFFDLLLPYGRTNSLPIKAEELNQYSTLFFEHDLYPGKKMILGNAQSNEKMLRPFTKPTQGVTDSIWL 109

[Close]

BLAST of GT626222 vs. TrEMBL
Match: Q45R26_MEDSA (Embryonic abundant protein-like OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 3.911e-40
Identity = 80/109 (73.39%), Postives = 92/109 (84.40%), Query Frame = 2

Query:  464 MEFAHLTVLSLFCLAFVGITAT-SSGEDYWQSVWPNTPLPKTFYDLLIPSGKTNSLPIKPEELNQYSTLFFKHDLHPGKIFTLGNTHSVGNTIRPFTKSKQGVTDSIWL 787
            MEFAHL++L+LFCLAFV I A+ SS EDYWQSVWPNTPLPK F+DLL+P G+TNSLPIK EELNQYSTLFF+HDL+PGK   LGN  S    +RPFT+ +QGVTDSIWL
Sbjct:    1 MEFAHLSILALFCLAFVRIGASPSSAEDYWQSVWPNTPLPKAFFDLLLPYGRTNSLPIKAEELNQYSTLFFEHDLYPGKKMILGNAQSNEKMLRPFTEPRQGVTDSIWL 109

[Close]

BLAST of GT626222 vs. TrEMBL
Match: Q04180_PEA (Putative uncharacterized protein (Fragment) OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 6.258e-30
Identity = 63/74 (85.14%), Postives = 67/74 (90.54%), Query Frame = 2

Query:  566 NTPLPKTFYDLLIPSGKTNSLPIKPEELNQYSTLFFKHDLHPGKIFTLGNTHSVGNTIRPFTKSKQGVTDSIWL 787
            NTPLPKTF DLLIP+GKTNSLPIK EELNQYSTLFF+HDLHPGK F LGN+H VGN IRPF KS+QGVTDSIWL
Sbjct:    1 NTPLPKTFSDLLIPNGKTNSLPIKTEELNQYSTLFFEHDLHPGKKFVLGNSHPVGNIIRPFRKSRQGVTDSIWL 74

[Close]

BLAST of GT626222 vs. TrEMBL
Match: Q04181_PEA (Putative uncharacterized protein (Fragment) OS=Pisum sativum PE=4 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 4.056e-29
Identity = 61/74 (82.43%), Postives = 66/74 (89.19%), Query Frame = 2

Query:  566 NTPLPKTFYDLLIPSGKTNSLPIKPEELNQYSTLFFKHDLHPGKIFTLGNTHSVGNTIRPFTKSKQGVTDSIWL 787
            NTPLPKTF DLLIP+GKTN LPIK +ELNQYSTLFF+HDLHPGK F LGN+H VGN IRPF KS+QGVTDSIWL
Sbjct:    1 NTPLPKTFSDLLIPNGKTNGLPIKTQELNQYSTLFFEHDLHPGKKFVLGNSHPVGNIIRPFRKSRQGVTDSIWL 74

[Close]

BLAST of GT626222 vs. TrEMBL
Match: C6T2D7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 1.308e-19
Identity = 49/83 (59.04%), Postives = 59/83 (71.08%), Query Frame = 2

Query:  464 MEFAHLTVLSLFCLAFVGITATSSGEDYWQSVWPNTPLPKTFYDLLIPSGKTNSLPIKPEELNQYSTLFFKHDLHPGKIFTLG 712
            ME  HL VL+L C+   G  A+ S +DYW SV+PNT LPK  +DL++PS KTN +PIK EE  QY TLFF+HDLHP KI  LG
Sbjct:    1 MEIGHLIVLALLCVTLKGADASQSAKDYWHSVFPNTRLPKPLWDLVLPSPKTN-MPIKVEEEKQYWTLFFEHDLHPRKIMHLG 82

[Close]

BLAST of GT626222 vs. TrEMBL
Match: Q6L460_SOLDE (ATP synthase D chain, mitochondrial, putative OS=Solanum demissum GN=SDM1_23t00004 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 8.477e-19
Identity = 45/50 (90.00%), Postives = 48/50 (96.00%), Query Frame = 2

Query:    5 KESERLEKEIAEVQELKKNISTMTADEYFAKHPELKKKFDDEIRNDQWGY 154
            KESERLEKE+AEVQELKK +STMTA+EYFAKHPELKKKFDDEIRND WGY
Sbjct:  119 KESERLEKEVAEVQELKKKLSTMTAEEYFAKHPELKKKFDDEIRNDYWGY 168

[Close]

BLAST of GT626222 vs. TrEMBL
Match: B9SK23_RICCO (ATP synthase D chain, mitochondrial, putative OS=Ricinus communis GN=RCOM_1399280 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 8.477e-19
Identity = 46/50 (92.00%), Postives = 47/50 (94.00%), Query Frame = 2

Query:    5 KESERLEKEIAEVQELKKNISTMTADEYFAKHPELKKKFDDEIRNDQWGY 154
            KESERLEKEIAEVQELKK +STMTADEYF KHPELKKKFDDEIRND WGY
Sbjct:  119 KESERLEKEIAEVQELKKKLSTMTADEYFEKHPELKKKFDDEIRNDYWGY 168

[Close]

BLAST of GT626222 vs. TrEMBL
Match: B3TLP5_ELAGV (Mitochondrial F0 ATP synthase D chain OS=Elaeis guineensis var. tenera PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 8.477e-19
Identity = 46/50 (92.00%), Postives = 47/50 (94.00%), Query Frame = 2

Query:    5 KESERLEKEIAEVQELKKNISTMTADEYFAKHPELKKKFDDEIRNDQWGY 154
            KESERLEKEIAEVQE+KK ISTMTADEYF KHPELKKKFDDEIRND WGY
Sbjct:  120 KESERLEKEIAEVQEMKKKISTMTADEYFEKHPELKKKFDDEIRNDYWGY 169

[Close]

BLAST of GT626222 vs. TrEMBL
Match: Q7XXS0_ORYSJ (Os08g0478200 protein OS=Oryza sativa subsp. japonica GN=Os08g0478200 PE=2 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 1.446e-18
Identity = 43/50 (86.00%), Postives = 49/50 (98.00%), Query Frame = 2

Query:    5 KESERLEKEIAEVQELKKNISTMTADEYFAKHPELKKKFDDEIRNDQWGY 154
            KESER+EKE+AE+QE+KKNISTMTADEYFAKHPE+K+KFDDEIRND WGY
Sbjct:  120 KESERIEKELAELQEMKKNISTMTADEYFAKHPEVKQKFDDEIRNDNWGY 169

[Close]

BLAST of GT626222 vs. SwissProt
Match: EA30_VICFA (Embryonic abundant protein VF30.1 OS=Vicia faba PE=2 SV=1)

HSP 1 Score: 205.297 bits (521), Expect = 3.194e-52
Identity = 97/108 (89.81%), Postives = 102/108 (94.44%), Query Frame = 2

Query:  464 MEFAHLTVLSLFCLAFVGITATSSGEDYWQSVWPNTPLPKTFYDLLIPSGKTNSLPIKPEELNQYSTLFFKHDLHPGKIFTLGNTHSVGNTIRPFTKSKQGVTDSIWL 787
            MEFAHLTVLSLFCLAFVGITATSSGEDYWQS+WPNTPLPKTF DLLIPSGKTNSLPIK EEL QYSTLFF+HDLHPGK F LGNT+SVG+ IRPFTKS+QGVTDSIWL
Sbjct:    1 MEFAHLTVLSLFCLAFVGITATSSGEDYWQSIWPNTPLPKTFSDLLIPSGKTNSLPIKSEELKQYSTLFFEHDLHPGKNFILGNTNSVGSIIRPFTKSRQGVTDSIWL 108

[Close]

BLAST of GT626222 vs. SwissProt
Match: SVF30_VICFA (Unknown seed protein 30.1 OS=Vicia faba PE=2 SV=1)

HSP 1 Score: 199.904 bits (507), Expect = 1.342e-50
Identity = 95/108 (87.96%), Postives = 100/108 (92.59%), Query Frame = 2

Query:  464 MEFAHLTVLSLFCLAFVGITATSSGEDYWQSVWPNTPLPKTFYDLLIPSGKTNSLPIKPEELNQYSTLFFKHDLHPGKIFTLGNTHSVGNTIRPFTKSKQGVTDSIWL 787
            MEFAHLTVLSLFCLAFVGITATSSGEDYWQS+WPNTPLPKTF DL IPSGKTNSLPIK EEL QYSTLFF+HDLHP K F LGNT+SVG+ IRPFTKS+QGVTDSIWL
Sbjct:    1 MEFAHLTVLSLFCLAFVGITATSSGEDYWQSIWPNTPLPKTFSDLSIPSGKTNSLPIKSEELKQYSTLFFEHDLHPRKNFILGNTNSVGSIIRPFTKSRQGVTDSIWL 108

[Close]

BLAST of GT626222 vs. SwissProt
Match: EA92_VICFA (Embryonic abundant protein USP92 OS=Vicia faba PE=2 SV=1)

HSP 1 Score: 199.134 bits (505), Expect = 2.289e-50
Identity = 95/108 (87.96%), Postives = 100/108 (92.59%), Query Frame = 2

Query:  464 MEFAHLTVLSLFCLAFVGITATSSGEDYWQSVWPNTPLPKTFYDLLIPSGKTNSLPIKPEELNQYSTLFFKHDLHPGKIFTLGNTHSVGNTIRPFTKSKQGVTDSIWL 787
            MEFAHLTVLSLFCLAFVGITATSS EDYWQS+WPNTPLPKTF DLLIPSGKTNSLPIK EEL QYSTLFF+HDLHP K F LGNT+SVG+ IRPFTKS+QGVTDSIWL
Sbjct:    1 MEFAHLTVLSLFCLAFVGITATSSEEDYWQSIWPNTPLPKTFSDLLIPSGKTNSLPIKSEELKQYSTLFFEHDLHPRKNFILGNTNSVGSIIRPFTKSRQGVTDSIWL 108

[Close]

BLAST of GT626222 vs. SwissProt
Match: EA87_VICFA (Embryonic abundant protein USP87 OS=Vicia faba PE=2 SV=1)

HSP 1 Score: 196.438 bits (498), Expect = 1.484e-49
Identity = 93/108 (86.11%), Postives = 99/108 (91.67%), Query Frame = 2

Query:  464 MEFAHLTVLSLFCLAFVGITATSSGEDYWQSVWPNTPLPKTFYDLLIPSGKTNSLPIKPEELNQYSTLFFKHDLHPGKIFTLGNTHSVGNTIRPFTKSKQGVTDSIWL 787
            MEFAHLTVLSLFCLAFVGITATS  EDYWQS+WPNTPLPKTF D+LIPSGKTNSLPIK EEL QYSTLFF+HDLHP K F LGNT+SVG+ IRPFTKS+QGVTDSIWL
Sbjct:    1 MEFAHLTVLSLFCLAFVGITATSPREDYWQSIWPNTPLPKTFSDMLIPSGKTNSLPIKSEELKQYSTLFFEHDLHPRKNFILGNTNSVGSIIRPFTKSRQGVTDSIWL 108

[Close]

BLAST of GT626222 vs. SwissProt
Match: ATP5H_ARATH (ATP synthase subunit d, mitochondrial OS=Arabidopsis thaliana GN=At3g52300 PE=1 SV=3)

HSP 1 Score: 94.7449 bits (234), Expect = 6.079e-19
Identity = 43/50 (86.00%), Postives = 46/50 (92.00%), Query Frame = 2

Query:    5 KESERLEKEIAEVQELKKNISTMTADEYFAKHPELKKKFDDEIRNDQWGY 154
            KESERLEKEIA+VQE+ K +STMTADEYF KHPELKKKFDDEIRND WGY
Sbjct:  119 KESERLEKEIADVQEISKKLSTMTADEYFEKHPELKKKFDDEIRNDNWGY 168

[Close]

BLAST of GT626222 vs. TAIR peptide
Match: AT3G52300.1 (| Symbols: ATPQ | ATP synthase D chain, mitochondrial | chr3:19396689-19398119 FORWARD LENGTH=168)

HSP 1 Score: 94.7449 bits (234), Expect = 6.047e-20
Identity = 43/50 (86.00%), Postives = 46/50 (92.00%), Query Frame = 2

Query:    5 KESERLEKEIAEVQELKKNISTMTADEYFAKHPELKKKFDDEIRNDQWGY 154
            KESERLEKEIA+VQE+ K +STMTADEYF KHPELKKKFDDEIRND WGY
Sbjct:  119 KESERLEKEIADVQEISKKLSTMTADEYFEKHPELKKKFDDEIRNDNWGY 168

The following BLAST results are available for this feature:

BLAST of GT626222 vs. TrEMBL
BLAST of GT626222 vs. SwissProt
BLAST of GT626222 vs. TAIR peptide

BLAST of GT626222 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q43675_VICFA	1.213e-49	87.96	Putative uncharacterized protein USP OS=Vicia faba...	[more]
Q45NP1_MEDSA	2.293e-40	75.23	Embryonic abundant protein-like protein (Fragment)...	[more]
Q45R26_MEDSA	3.911e-40	73.39	Embryonic abundant protein-like OS=Medicago sativa...	[more]
Q04180_PEA	6.258e-30	85.14	Putative uncharacterized protein (Fragment) OS=Pis...	[more]
Q04181_PEA	4.056e-29	82.43	Putative uncharacterized protein (Fragment) OS=Pis...	[more]
C6T2D7_SOYBN	1.308e-19	59.04	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q6L460_SOLDE	8.477e-19	90.00	ATP synthase D chain, mitochondrial, putative OS=S...	[more]
B9SK23_RICCO	8.477e-19	92.00	ATP synthase D chain, mitochondrial, putative OS=R...	[more]
B3TLP5_ELAGV	8.477e-19	92.00	Mitochondrial F0 ATP synthase D chain OS=Elaeis gu...	[more]
Q7XXS0_ORYSJ	1.446e-18	86.00	Os08g0478200 protein OS=Oryza sativa subsp. japoni...	[more]

BLAST of GT626222 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 5


Match Name	E-value	Identity	Description
EA30_VICFA	3.194e-52	89.81	Embryonic abundant protein VF30.1 OS=Vicia faba PE...	[more]
SVF30_VICFA	1.342e-50	87.96	Unknown seed protein 30.1 OS=Vicia faba PE=2 SV=1	[more]
EA92_VICFA	2.289e-50	87.96	Embryonic abundant protein USP92 OS=Vicia faba PE=...	[more]
EA87_VICFA	1.484e-49	86.11	Embryonic abundant protein USP87 OS=Vicia faba PE=...	[more]
ATP5H_ARATH	6.079e-19	86.00	ATP synthase subunit d, mitochondrial OS=Arabidops...	[more]

BLAST of GT626222 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT3G52300.1	6.047e-20	86.00	\| Symbols: ATPQ \| ATP synthase D chain, mitochondr...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004873	BURP	PROFILE	PS51277	BURP	coord: 108..148 score: 13
None	No IPR available	SEG	seg	seg	coord: 4..16 score

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT626222 ID=GT626222; Name=GT626222; organism=Lens culinaris; type=EST; length=787bp
TTGGAAGGAGTCTGAGCGCTTGGAAAAGGAAATTGCCGAAGTACAAGAGT
TGAAGAAAAATATCAGTACCATGACAGCAGACGAGTACTTTGCCAAGCAT
CCTGAGCTTAAGAAGAAATTTGATGATGAGATTAGGAACGACCAGTGGGG
CTATTGATTTTCAGAACTTCAAAGTGTAGCAAAACATCCGAAGTTGATGT
TTTCCTTGCTGGTTTAATAAATAATAACTACATTGATTTTTTTGTAACTT
GGTTGTAACTTGGTTCTCCTCTTCAGTTTAAAAGGTTCTTTACATTTCCA
TTTTCAAAGTTGTCAAAATTTGATGGAGGCTGTTGGAGTTTAGAAACTGC
TGTTTTATTATGGTTTCAGTTCAGACACCCACTTTCAGAAAAATGCTGTG
GTTCTGTTAATAAATTGTTGGGCTTTGGTAAATCACCATTCTCATTTCTT
CATAGCCAGTCAAATGGAGTTTGCACATCTCACAGTTTTATCTCTTTTTT
GTTTGGCTTTCGTGGGAATCACTGCAACATCATCAGGAGAAGATTATTGG
CAATCTGTTTGGCCAAACACTCCATTGCCAAAGACCTTCTATGATCTATT
AATTCCCAGTGGAAAGACTAATAGTCTACCCATTAAACCAGAAGAATTGA
ACCAATACTCGACGCTCTTTTTTAAACACGACCTTCACCCTGGGAAAATA
TTTACTTTGGGTAACACTCATTCGGTTGGAAATACAATTCGACCATTCAC
AAAATCAAAACAAGGTGTCACAGATTCCATATGGCTA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR004873	BURP_dom

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