GT627153

EST Overview
NameGT627153
Unique NameGT627153
TypeEST
OrganismLens culinaris (lentil)
Sequence length772
Libraries
Library NameType
LIBEST_025390 LCUSE1NGcdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Lens culinaris unigene v1Lens culinaris unigene v1EST
Homology
[Close]
BLAST of GT627153 vs. TrEMBL
Match: B9I2W0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_834296 PE=4 SV=1)

HSP 1 Score: 467.618 bits (1202), Expect = 5.111e-130
Identity = 228/256 (89.06%), Postives = 242/256 (94.53%), Query Frame = 1 
Query:    1 VERQGGLTTVLHHIEHAAVTILDRWKVTISNPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSIAHHGQAFMLKRAAEEPLGHAAAMITDVLENAETNNVISASQKRALL 768
            VERQGGL T+LHHIEHAAVTILDRWKVTI   Q KQ LQPPK+MNNYLG+GCDAKVAL+IHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTF DFPWQ+RV VDGV+IEVPEDAEGVLVANIGSYMGGVDLWQNEDE YDNFDPQSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQGQSIKI L A  PVQIDGEPWFQ+PCT+ ++HHGQAFMLKRAAEEPLGHAAA+ITDVLENAETN+VI+ASQKRALL
Sbjct:  463 VERQGGLCTLLHHIEHAAVTILDRWKVTIVKNQRKQ-LQPPKYMNNYLGVGCDAKVALEIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFADFPWQVRVEVDGVDIEVPEDAEGVLVANIGSYMGGVDLWQNEDETYDNFDPQSMHDKMLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKILLLAALPVQIDGEPWFQQPCTLHVSHHGQAFMLKRAAEEPLGHAAAIITDVLENAETNHVINASQKRALL 717
[Close]
BLAST of GT627153 vs. TrEMBL
Match: D7SI39_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_0.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00007909001 PE=4 SV=1)

HSP 1 Score: 464.537 bits (1194), Expect = 4.327e-129
Identity = 229/257 (89.11%), Postives = 243/257 (94.55%), Query Frame = 1 
Query:    1 VERQGGLTTVLHHIEHAAVTILDRWKVTISNPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFPVQIDGEPWFQEP-CTVSIAHHGQAFMLKRAAEEPLGHAAAMITDVLENAETNNVISASQKRALL 768
            VERQGGL TVLHHIEHAAVT+LDRWK+TI   QGKQ LQ PKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAK+IMDRTF DFPWQ+RV VDGVE+EVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQGQSIKI LFA FP+QIDGEPWFQE  CT++I+HHGQAFMLKR +EEPLGHAAA+ITDVLENAETN++ISASQKR LL
Sbjct:  469 VERQGGLCTVLHHIEHAAVTMLDRWKITILQ-QGKQ-LQAPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKNIMDRTFADFPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKMLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIHLFAPFPIQIDGEPWFQEQLCTLTISHHGQAFMLKRVSEEPLGHAAAIITDVLENAETNHIISASQKRTLL 723
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BLAST of GT627153 vs. TrEMBL
Match: B9RLR5_RICCO (Diacylglycerol kinase, theta, putative OS=Ricinus communis GN=RCOM_1470420 PE=4 SV=1)

HSP 1 Score: 464.151 bits (1193), Expect = 5.651e-129
Identity = 224/256 (87.50%), Postives = 242/256 (94.53%), Query Frame = 1 
Query:    1 VERQGGLTTVLHHIEHAAVTILDRWKVTISNPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSIAHHGQAFMLKRAAEEPLGHAAAMITDVLENAETNNVISASQKRALL 768
            VERQGGL T+L HIEHAAVTILDRWKV I N QGKQ L  PKFMNNYLG+GCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGA+SIMDRTF DFPWQ+RV VDGVEIEVPEDAEGVL+ANIGSYMGGVDLWQNEDE+YDNFDPQSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQL A  PVQIDGEPWFQ+PCT++++HHGQAF+LKR +EEPLGHAAA+ITDVLENAE+N+VI+ASQKRALL
Sbjct:  462 VERQGGLCTLLQHIEHAAVTILDRWKVAIVNHQGKQ-LMSPKFMNNYLGVGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRTFADFPWQVRVEVDGVEIEVPEDAEGVLIANIGSYMGGVDLWQNEDESYDNFDPQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLLAPLPVQIDGEPWFQQPCTLAVSHHGQAFLLKRVSEEPLGHAAAIITDVLENAESNHVINASQKRALL 716
[Close]
BLAST of GT627153 vs. TrEMBL
Match: A5BDX3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_021450 PE=4 SV=1)

HSP 1 Score: 460.685 bits (1184), Expect = 6.248e-128
Identity = 227/257 (88.33%), Postives = 242/257 (94.16%), Query Frame = 1 
Query:    1 VERQGGLTTVLHHIEHAAVTILDRWKVTISNPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFPVQIDGEPWFQ-EPCTVSIAHHGQAFMLKRAAEEPLGHAAAMITDVLENAETNNVISASQKRALL 768
            VERQGGL TVLHHIEHAAVT+LDRWK+TI   QGKQ LQ PKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAK+IMDRTF DFPWQ+RV VDGVE+EVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQGQSIKI LFA FP+QIDGEPWFQ + CT++I+HHGQAFMLKR +EEPLGHAAA+ITDVLENAETN++ISA QKR LL
Sbjct:  443 VERQGGLCTVLHHIEHAAVTMLDRWKITILQ-QGKQ-LQAPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKNIMDRTFADFPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKMLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIHLFAPFPIQIDGEPWFQGQLCTLTISHHGQAFMLKRVSEEPLGHAAAIITDVLENAETNHIISAXQKRTLL 697
[Close]
BLAST of GT627153 vs. TrEMBL
Match: D7M0N5_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_487625 PE=4 SV=1)

HSP 1 Score: 458.759 bits (1179), Expect = 2.374e-127
Identity = 220/256 (85.94%), Postives = 244/256 (95.31%), Query Frame = 1 
Query:    1 VERQGGLTTVLHHIEHAAVTILDRWKVTISNPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSIAHHGQAFMLKRAAEEPLGHAAAMITDVLENAETNNVISASQKRALL 768
            VERQGGL+TVL +IEHAAVT+LDRWKV+I N QGKQ LQPPK+MNNY+G+GCDAKVAL+IHNLREENPE+FY+QFMNKVLYAREGA+SIMDRTFEDFPWQ+RV VDGV+IEVPEDAEG+LVANIGSYMGGVDLWQNEDE Y+NFDPQSMHDKI+EVVSISGTWHLGKLQVGLSRARRLAQG ++KIQL A  PVQIDGEPW Q+PCT++I+HHGQAFMLKRAAEEPLGHAAA+ITDVLENAETN VI+ASQKRALL
Sbjct:  466 VERQGGLSTVLQNIEHAAVTVLDRWKVSILNQQGKQ-LQPPKYMNNYIGVGCDAKVALEIHNLREENPERFYSQFMNKVLYAREGARSIMDRTFEDFPWQVRVEVDGVDIEVPEDAEGILVANIGSYMGGVDLWQNEDETYENFDPQSMHDKIVEVVSISGTWHLGKLQVGLSRARRLAQGSAVKIQLCAPLPVQIDGEPWNQQPCTLTISHHGQAFMLKRAAEEPLGHAAAIITDVLENAETNQVINASQKRALL 720
[Close]
BLAST of GT627153 vs. TrEMBL
Match: Q75GS1_ORYSJ (Putative diacylglycerol kinase (With alternative splicing) OS=Oryza sativa subsp. japonica GN=OSJNBa0020H02.13 PE=4 SV=1)

HSP 1 Score: 420.239 bits (1079), Expect = 9.359e-116
Identity = 201/256 (78.52%), Postives = 232/256 (90.62%), Query Frame = 1 
Query:    1 VERQGGLTTVLHHIEHAAVTILDRWKVTISNPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSIAHHGQAFMLKRAAEEPLGHAAAMITDVLENAETNNVISASQKRALL 768
            VE+QGGL TVLH IEHAAVTILDRWKV I + +GK  L   K+MNNYLGIGCDAKVALDIHNLREENPEKFY+QF+NKVLYAREGAKS++DRTF D PWQ+R+ VDG EIE+PED+EGVLVANI SYMGGVDLW++ED+N DNFDPQS+HDK++EVVSISGTWHLG LQVGLSRARR+AQGQSIKIQ+FA FPVQ+DGEPW Q PCT+ I+HHGQAFML+R  EE LGHAAA++TDVLENAE++++I+ASQKRALL
Sbjct:  477 VEKQGGLCTVLHDIEHAAVTILDRWKVAIEDKRGKNVLMV-KYMNNYLGIGCDAKVALDIHNLREENPEKFYSQFLNKVLYAREGAKSMIDRTFVDLPWQVRLEVDGTEIEIPEDSEGVLVANIPSYMGGVDLWKSEDDNPDNFDPQSIHDKMVEVVSISGTWHLGTLQVGLSRARRIAQGQSIKIQIFAPFPVQVDGEPWTQNPCTLKISHHGQAFMLRRTIEESLGHAAAIVTDVLENAESSHLITASQKRALL 731
[Close]
BLAST of GT627153 vs. TrEMBL
Match: C7IZW2_ORYSJ (Os03g0425300 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os03g0425300 PE=4 SV=1)

HSP 1 Score: 420.239 bits (1079), Expect = 9.359e-116
Identity = 201/256 (78.52%), Postives = 232/256 (90.62%), Query Frame = 1 
Query:    1 VERQGGLTTVLHHIEHAAVTILDRWKVTISNPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSIAHHGQAFMLKRAAEEPLGHAAAMITDVLENAETNNVISASQKRALL 768
            VE+QGGL TVLH IEHAAVTILDRWKV I + +GK  L   K+MNNYLGIGCDAKVALDIHNLREENPEKFY+QF+NKVLYAREGAKS++DRTF D PWQ+R+ VDG EIE+PED+EGVLVANI SYMGGVDLW++ED+N DNFDPQS+HDK++EVVSISGTWHLG LQVGLSRARR+AQGQSIKIQ+FA FPVQ+DGEPW Q PCT+ I+HHGQAFML+R  EE LGHAAA++TDVLENAE++++I+ASQKRALL
Sbjct:   76 VEKQGGLCTVLHDIEHAAVTILDRWKVAIEDKRGKNVLMV-KYMNNYLGIGCDAKVALDIHNLREENPEKFYSQFLNKVLYAREGAKSMIDRTFVDLPWQVRLEVDGTEIEIPEDSEGVLVANIPSYMGGVDLWKSEDDNPDNFDPQSIHDKMVEVVSISGTWHLGTLQVGLSRARRIAQGQSIKIQIFAPFPVQVDGEPWTQNPCTLKISHHGQAFMLRRTIEESLGHAAAIVTDVLENAESSHLITASQKRALL 330
[Close]
BLAST of GT627153 vs. TrEMBL
Match: B9F945_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_11338 PE=4 SV=1)

HSP 1 Score: 420.239 bits (1079), Expect = 9.359e-116
Identity = 201/256 (78.52%), Postives = 232/256 (90.62%), Query Frame = 1 
Query:    1 VERQGGLTTVLHHIEHAAVTILDRWKVTISNPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSIAHHGQAFMLKRAAEEPLGHAAAMITDVLENAETNNVISASQKRALL 768
            VE+QGGL TVLH IEHAAVTILDRWKV I + +GK  L   K+MNNYLGIGCDAKVALDIHNLREENPEKFY+QF+NKVLYAREGAKS++DRTF D PWQ+R+ VDG EIE+PED+EGVLVANI SYMGGVDLW++ED+N DNFDPQS+HDK++EVVSISGTWHLG LQVGLSRARR+AQGQSIKIQ+FA FPVQ+DGEPW Q PCT+ I+HHGQAFML+R  EE LGHAAA++TDVLENAE++++I+ASQKRALL
Sbjct:  400 VEKQGGLCTVLHDIEHAAVTILDRWKVAIEDKRGKNVLMV-KYMNNYLGIGCDAKVALDIHNLREENPEKFYSQFLNKVLYAREGAKSMIDRTFVDLPWQVRLEVDGTEIEIPEDSEGVLVANIPSYMGGVDLWKSEDDNPDNFDPQSIHDKMVEVVSISGTWHLGTLQVGLSRARRIAQGQSIKIQIFAPFPVQVDGEPWTQNPCTLKISHHGQAFMLRRTIEESLGHAAAIVTDVLENAESSHLITASQKRALL 654
[Close]
BLAST of GT627153 vs. TrEMBL
Match: B8AK66_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_12126 PE=4 SV=1)

HSP 1 Score: 420.239 bits (1079), Expect = 9.359e-116
Identity = 201/256 (78.52%), Postives = 232/256 (90.62%), Query Frame = 1 
Query:    1 VERQGGLTTVLHHIEHAAVTILDRWKVTISNPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSIAHHGQAFMLKRAAEEPLGHAAAMITDVLENAETNNVISASQKRALL 768
            VE+QGGL TVLH IEHAAVTILDRWKV I + +GK  L   K+MNNYLGIGCDAKVALDIHNLREENPEKFY+QF+NKVLYAREGAKS++DRTF D PWQ+R+ VDG EIE+PED+EGVLVANI SYMGGVDLW++ED+N DNFDPQS+HDK++EVVSISGTWHLG LQVGLSRARR+AQGQSIKIQ+FA FPVQ+DGEPW Q PCT+ I+HHGQAFML+R  EE LGHAAA++TDVLENAE++++I+ASQKRALL
Sbjct:  400 VEKQGGLCTVLHDIEHAAVTILDRWKVAIEDKRGKNVLMV-KYMNNYLGIGCDAKVALDIHNLREENPEKFYSQFLNKVLYAREGAKSMIDRTFVDLPWQVRLEVDGTEIEIPEDSEGVLVANIPSYMGGVDLWKSEDDNPDNFDPQSIHDKMVEVVSISGTWHLGTLQVGLSRARRIAQGQSIKIQIFAPFPVQVDGEPWTQNPCTLKISHHGQAFMLRRTIEESLGHAAAIVTDVLENAESSHLITASQKRALL 654
[Close]
BLAST of GT627153 vs. TrEMBL
Match: C5WWI1_SORBI (Putative uncharacterized protein Sb01g032250 OS=Sorghum bicolor GN=Sb01g032250 PE=4 SV=1)

HSP 1 Score: 417.542 bits (1072), Expect = 6.066e-115
Identity = 201/256 (78.52%), Postives = 229/256 (89.45%), Query Frame = 1 
Query:    1 VERQGGLTTVLHHIEHAAVTILDRWKVTISNPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSIAHHGQAFMLKRAAEEPLGHAAAMITDVLENAETNNVISASQKRALL 768
            VE+QGGL TVLH IEHAAVTILDRWKV + + + K  +   K+MNNYLGIGCDAKVALDIHNLREENPEKFY+QF+NKVLYAREGAKSI+DR F D PWQ+R+ VDG EIE+PED+EGVLVANI SYMGGVDLWQNE EN +NFDPQS+HDK+LEVVSI+G WHLG LQVGLSRARR+AQGQSIKIQ FA FPVQ+DGEPW Q+PCT+ I+HHGQAFML+RA EEPLGHAAAMITDVLE+AE++ VI+ASQK+ALL
Sbjct:  454 VEKQGGLCTVLHDIEHAAVTILDRWKVAVEDKKSKNVVLV-KYMNNYLGIGCDAKVALDIHNLREENPEKFYSQFLNKVLYAREGAKSIIDRAFVDLPWQVRLEVDGTEIEIPEDSEGVLVANIPSYMGGVDLWQNEGENPENFDPQSIHDKMLEVVSITGAWHLGTLQVGLSRARRIAQGQSIKIQTFAPFPVQVDGEPWVQQPCTLKISHHGQAFMLRRAIEEPLGHAAAMITDVLEHAESSRVITASQKKALL 708
[Close]
BLAST of GT627153 vs. SwissProt
Match: DGK1_ARATH (Diacylglycerol kinase 1 OS=Arabidopsis thaliana GN=DGK1 PE=1 SV=2)

HSP 1 Score: 457.218 bits (1175), Expect = 4.504e-128
Identity = 219/256 (85.55%), Postives = 243/256 (94.92%), Query Frame = 1 
Query:    1 VERQGGLTTVLHHIEHAAVTILDRWKVTISNPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSIAHHGQAFMLKRAAEEPLGHAAAMITDVLENAETNNVISASQKRALL 768
            VERQGGL+TVL +IEHAAVT+LDRWKV+I N QGKQ LQPPK+MNNY+G+GCDAKVAL+IHNLREENPE+FY+QFMNKVLYAREGA+SIMDRTFEDFPWQ+RV VDGV+IEVPEDAEG+LVANIGSYMGGVDLWQNEDE Y+NFDPQSMHDKI+EVVSISGTWHLGKLQVGLSRARRLAQG ++KIQL A  PVQIDGEPW Q+PCT++I+HHGQAFMLKRAAEEPLGHAAA+ITDVLENAETN VI+ASQKR LL
Sbjct:  466 VERQGGLSTVLQNIEHAAVTVLDRWKVSILNQQGKQ-LQPPKYMNNYIGVGCDAKVALEIHNLREENPERFYSQFMNKVLYAREGARSIMDRTFEDFPWQVRVEVDGVDIEVPEDAEGILVANIGSYMGGVDLWQNEDETYENFDPQSMHDKIVEVVSISGTWHLGKLQVGLSRARRLAQGSAVKIQLCAPLPVQIDGEPWNQQPCTLTISHHGQAFMLKRAAEEPLGHAAAIITDVLENAETNQVINASQKRTLL 720
[Close]
BLAST of GT627153 vs. SwissProt
Match: DGKE_MOUSE (Diacylglycerol kinase epsilon OS=Mus musculus GN=Dgke PE=2 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 2.901e-34
Identity = 84/221 (38.01%), Postives = 131/221 (59.28%), Query Frame = 1 
Query:   28 VLHHIEHAAVTILDRWKVTISNPQGKQQLQPPK--FMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQN-EDENYDNFDPQSMHDK-ILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQL-FAKFPVQIDGEPWFQEPCTVSIAHHGQAFMLKRAAEE 675
            VL ++  A    LDRWKV ++N +G   L+ PK   MNNY  +G DA +AL+ H  RE+ P  F ++ +NK +Y   G K  + +  +D   +I + +DG  +E+P + EG++V NIG + GG  LW+   DE Y    P + HD  +LE+V + G++H  ++QV L+   R+ Q  ++++ L  +  P+Q+DGEPW Q PCTV+I H   A ML  + E+
Sbjct:  332 VLRNVMEADGIKLDRWKVQVTN-KGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKIELELDGERVELP-NLEGIIVLNIGYWGGGCRLWEGMGDETY----PLARHDDGLLEIVGVYGSFHCAQIQVKLANPFRIGQAHTVRLTLKCSMMPMQVDGEPWAQGPCTVTITHKTHALMLYFSGEQ 546
[Close]
BLAST of GT627153 vs. SwissProt
Match: DGKQ_MOUSE (Diacylglycerol kinase theta OS=Mus musculus GN=Dgkq PE=1 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 6.463e-34
Identity = 83/227 (36.56%), Postives = 130/227 (57.27%), Query Frame = 1 
Query:   25 TVLHHIEHAAVTILDRWKVTI------SNPQGKQQLQPPKF--MNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSI-AHHGQAFMLKRAAEEP 678
            +VL  ++ A   ++DRW + +      S      + +PPK   MNNY GIG DA+++LD H  REE P KF ++F NK +Y R G + I          +IR+ V+  E+E+P   EG++  NI S+  G DLW +++++   F+   + D +LEVV ++G  H+G++Q GL    R+AQG   ++ L    PVQ+DGEPW Q P  + I A   +  ML++A ++P
Sbjct:  691 SVLVSVDEADAVLMDRWTILLDAHEIDSTENNVVETEPPKIVQMNNYCGIGIDAELSLDFHQAREEEPGKFTSRFHNKGVYVRVGLQKISHS--RSLHKEIRLQVEQQEVELPS-IEGLIFINIPSWGSGADLWGSDNDS--RFEKPRIDDGLLEVVGVTGVVHMGQVQGGLRSGIRIAQGSYFRVTLLKATPVQVDGEPWVQAPGHMIISATAPKVHMLRKAKQKP 912
[Close]
BLAST of GT627153 vs. SwissProt
Match: DGKQ_HUMAN (Diacylglycerol kinase theta OS=Homo sapiens GN=DGKQ PE=1 SV=2)

HSP 1 Score: 142.895 bits (359), Expect = 1.880e-33
Identity = 83/227 (36.56%), Postives = 127/227 (55.95%), Query Frame = 1 
Query:   25 TVLHHIEHAAVTILDRWKVTI------SNPQGKQQLQPPKF--MNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSIAHHG-QAFMLKRAAEEP 678
            +VL  ++ A   ++DRW + +      S        +PPK   M+NY GIG DA+++LD H  REE P KF ++  NK +Y R G + I          QIR+ V+  E+E+P   EG++  NI S+  G DLW ++ +    F+   M D +LEVV ++G  H+G++Q GL    R+AQG   ++ L    PVQ+DGEPW Q P  + I+  G +  ML++A ++P
Sbjct:  699 SVLLSVDEADAVLMDRWTILLDAHEAGSAENDTADAEPPKIVQMSNYCGIGIDAELSLDFHQAREEEPGKFTSRLHNKGVYVRVGLQKISHS--RSLHKQIRLQVERQEVELPS-IEGLIFINIPSWGSGADLWGSDSDT--RFEKPRMDDGLLEVVGVTGVVHMGQVQGGLRSGIRIAQGSYFRVTLLKATPVQVDGEPWVQAPGHMIISAAGPKVHMLRKAKQKP 920
[Close]
BLAST of GT627153 vs. SwissProt
Match: DGKE_HUMAN (Diacylglycerol kinase epsilon OS=Homo sapiens GN=DGKE PE=1 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 3.207e-33
Identity = 83/221 (37.56%), Postives = 129/221 (58.37%), Query Frame = 1 
Query:   28 VLHHIEHAAVTILDRWKVTISNPQGKQQLQPPK--FMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQN-EDENYDNFDPQSMHDK-ILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQL-FAKFPVQIDGEPWFQEPCTVSIAHHGQAFMLKRAAEE 675
            VL ++  A    LDRWKV ++N +G   L+ PK   MNNY  +G DA +AL+ H  RE+ P  F ++ +NK +Y   G K  + +  +D   ++ + +DG  + +P   EG++V NIG + GG  LW+   DE Y    P + HD  +LEVV + G++H  ++QV L+   R+ Q  ++++ L  +  P+Q+DGEPW Q PCTV+I H   A ML  + E+
Sbjct:  335 VLRNVMEADGIKLDRWKVQVTN-KGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKVELELDGERVALP-SLEGIIVLNIGYWGGGCRLWEGMGDETY----PLARHDDGLLEVVGVYGSFHCAQIQVKLANPFRIGQAHTVRLILKCSMMPMQVDGEPWAQGPCTVTITHKTHAMMLYFSGEQ 549
[Close]
BLAST of GT627153 vs. SwissProt
Match: DGKG_RAT (Diacylglycerol kinase gamma OS=Rattus norvegicus GN=Dgkg PE=2 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 1.032e-31
Identity = 82/234 (35.04%), Postives = 119/234 (50.85%), Query Frame = 1 
Query:   13 GGLTTVLHHIEHAAVTILDRWKVTISNPQGKQQLQ--PPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPE-DAEGVLVANIGSYMGGVDLWQNEDEN--------YDNFDP-------QSMHDKILEVVSISGTWHLGKLQVGL-SRARRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSIAHHGQAFML 657
            G LT +L  IE + + +LDRW + +   +  +     P   MNNY  IG DA +A   H +RE++PEKF ++  NK+ Y   G       T +     I +  DGVE+++     EG+ + NI S  GG +LW    +N            DP       Q + D++LEVV + G   +G++  GL S  RRLAQ  S+ I+     P+Q+DGEPW Q PC + I H  QA M+
Sbjct:  535 GSLTKILKEIEQSPLVMLDRWYLEVMPREEVENGDQVPYNIMNNYFSIGVDASIAHRFHVMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVEVDLSNIFLEGIAILNIPSMYGGTNLWGETKKNRAVIRESRKSVTDPKELKCCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVTIRTKKLLPMQVDGEPWMQPPCMIKITHKNQAPMM 768
[Close]
BLAST of GT627153 vs. SwissProt
Match: DGKB_MOUSE (Diacylglycerol kinase beta OS=Mus musculus GN=Dgkb PE=2 SV=2)

HSP 1 Score: 137.117 bits (344), Expect = 1.032e-31
Identity = 83/238 (34.87%), Postives = 117/238 (49.16%), Query Frame = 1 
Query:   19 LTTVLHHIEHAAVTILDRWK--VTISNPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPE-DAEGVLVANIGSYMGGVDLWQNEDENYDN---------------------FDPQSMHDKILEVVSISGTWHLGKLQVGLSRA-RRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSIAHHGQAFML 657
            L  +L  IE +   +LDRWK  VT ++   K    P   +NNY  IG DA +A   H +RE++PEKF ++  NK  Y   G       T +     + +  DGV+I++     EG+ + NI S  GG +LW    +   +                     F  Q + D++LEVV + G   +G++  GL  A RRLAQ  S+ I+     P+QIDGEPW Q PCT+ I H  QA ML
Sbjct:  542 LMKILKDIESSTEIMLDRWKFEVTPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINISLEGIAILNIPSMHGGSNLWGESKKKRSHRRIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPML 779
[Close]
BLAST of GT627153 vs. SwissProt
Match: DGKG_MOUSE (Diacylglycerol kinase gamma OS=Mus musculus GN=Dgkg PE=2 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.348e-31
Identity = 82/234 (35.04%), Postives = 119/234 (50.85%), Query Frame = 1 
Query:   13 GGLTTVLHHIEHAAVTILDRWKVTISNPQGKQQLQ--PPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPE-DAEGVLVANIGSYMGGVDLWQNEDEN--------YDNFDP-------QSMHDKILEVVSISGTWHLGKLQVGL-SRARRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSIAHHGQAFML 657
            G LT +L  IE + + +LDRW + +   +  +     P   MNNY  IG DA +A   H +RE++PEKF ++  NK+ Y   G       T +     I +  DGVE+++     EG+ + NI S  GG +LW    +N            DP       Q + D++LEVV + G   +G++  GL S  RRLAQ  S+ I+     P+Q+DGEPW Q  CT+ I H  QA M+
Sbjct:  535 GSLTKILKEIEQSPLVMLDRWYLEVMPREEVENGDQVPYNIMNNYFSIGVDASIAHRFHMMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVEVDLSNIFLEGIAILNIPSMYGGTNLWGETKKNRAVIRESRKSVTDPKELKCCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVTIRTNKLLPMQVDGEPWMQPQCTIKITHKNQAPMM 768
[Close]
BLAST of GT627153 vs. SwissProt
Match: DGKH_DROMO (Diacylglycerol kinase eta OS=Drosophila mojavensis GN=GI24133 PE=3 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 2.299e-31
Identity = 68/180 (37.78%), Postives = 110/180 (61.11%), Query Frame = 1 
Query:  130 MNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFA--KFPVQIDGEPWFQEPCTVSIAHHGQAFMLKR 663
            MNNY GIG DAK++LD HN REE+PEK  ++  N + Y   G+K ++ +T ++   ++++  DG  I +PE  +G+++ NI S+MGG + W N  +  D F P S  D++LEVV++ G+  +   ++   +  R+AQ QS++I +    + P+Q+DGE W Q P  + I H  +  ML R
Sbjct: 1467 MNNYFGIGIDAKISLDFHNKREEHPEKCRSRARNYMWYGVLGSKQLLQKTCKNLEQRVQLECDGQRIPLPE-LQGIVILNIPSFMGGTNFWGNSSKKEDIFLPPSFDDRVLEVVAVFGSVQMAASRLINLQHHRIAQCQSVQINILGDEEIPIQVDGEAWLQPPGMIRILHKNRVQMLCR 1645
[Close]
BLAST of GT627153 vs. SwissProt
Match: DGKB_RAT (Diacylglycerol kinase beta OS=Rattus norvegicus GN=Dgkb PE=2 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 2.299e-31
Identity = 82/238 (34.45%), Postives = 117/238 (49.16%), Query Frame = 1 
Query:   19 LTTVLHHIEHAAVTILDRWK--VTISNPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPE-DAEGVLVANIGSYMGGVDLWQNEDENYDN---------------------FDPQSMHDKILEVVSISGTWHLGKLQVGLSRA-RRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSIAHHGQAFML 657
            L  +L  IE +   +LDRWK  VT ++   K    P   +NNY  IG DA +A   H +RE++PEKF ++  NK  Y   G       T +     + +  DGV+I++     +G+ + NI S  GG +LW    +   +                     F  Q + D++LEVV + G   +G++  GL  A RRLAQ  S+ I+     P+QIDGEPW Q PCT+ I H  QA ML
Sbjct:  541 LMKILKDIESSTEIMLDRWKFEVTPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINISLQGIAILNIPSMHGGSNLWGESKKKRSHRRIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPML 778
[Close]
BLAST of GT627153 vs. TAIR peptide
Match: AT5G07920.1 (| Symbols: DGK1, ATDGK1 | diacylglycerol kinase1 | chr5:2525197-2528396 REVERSE LENGTH=728)

HSP 1 Score: 457.218 bits (1175), Expect = 4.497e-129
Identity = 219/256 (85.55%), Postives = 243/256 (94.92%), Query Frame = 1 
Query:    1 VERQGGLTTVLHHIEHAAVTILDRWKVTISNPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSIAHHGQAFMLKRAAEEPLGHAAAMITDVLENAETNNVISASQKRALL 768
            VERQGGL+TVL +IEHAAVT+LDRWKV+I N QGKQ LQPPK+MNNY+G+GCDAKVAL+IHNLREENPE+FY+QFMNKVLYAREGA+SIMDRTFEDFPWQ+RV VDGV+IEVPEDAEG+LVANIGSYMGGVDLWQNEDE Y+NFDPQSMHDKI+EVVSISGTWHLGKLQVGLSRARRLAQG ++KIQL A  PVQIDGEPW Q+PCT++I+HHGQAFMLKRAAEEPLGHAAA+ITDVLENAETN VI+ASQKR LL
Sbjct:  466 VERQGGLSTVLQNIEHAAVTVLDRWKVSILNQQGKQ-LQPPKYMNNYIGVGCDAKVALEIHNLREENPERFYSQFMNKVLYAREGARSIMDRTFEDFPWQVRVEVDGVDIEVPEDAEGILVANIGSYMGGVDLWQNEDETYENFDPQSMHDKIVEVVSISGTWHLGKLQVGLSRARRLAQGSAVKIQLCAPLPVQIDGEPWNQQPCTLTISHHGQAFMLKRAAEEPLGHAAAIITDVLENAETNQVINASQKRTLL 720
[Close]
BLAST of GT627153 vs. TAIR peptide
Match: AT5G63770.2 (| Symbols: ATDGK2, DGK2 | diacylglycerol kinase 2 | chr5:25519755-25522404 FORWARD LENGTH=558)

HSP 1 Score: 317.39 bits (812), Expect = 5.561e-87
Identity = 155/258 (60.08%), Postives = 197/258 (76.36%), Query Frame = 1 
Query:    1 VERQGGLTTVLHHIEHAAVTILDRWKVTISNPQGKQ--QLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSIAHHGQAFMLKRAAEEPLGHAAAMITDVLENAETNNVISASQKRALL 768
            V+ QG L T L  I+HAAVT+LDRW V I     ++    +  KFM NYLGIGCDAKVA + H +R+E PEKF +QF+NK+ YA+EGA+ IMDR   D PWQ+ + VDG +IE+P+D+EG++V NIGSYMGGVDLWQN+ E+ DNF  Q MHDK LEVV + G WHLGKLQVGLS+ARRLAQG+ I+I + + FPVQIDGEP+ Q+P  + I HHGQ FML+RA++EP GHAAA++ +VL +AE   VI+ASQK+ LL
Sbjct:  293 VDGQGSLRTFLQDIDHAAVTMLDRWSVKIVEESTEKFPAREGHKFMMNYLGIGCDAKVAYEFHMMRQEKPEKFCSQFVNKLRYAKEGARDIMDRACADLPWQVWLEVDGKDIEIPKDSEGLIVLNIGSYMGGVDLWQNDYEHDDNFSIQCMHDKTLEVVCVRGAWHLGKLQVGLSQARRLAQGKVIRIHVSSPFPVQIDGEPFIQQPGCLEITHHGQVFMLRRASDEPRGHAAAIMNEVLLDAECKGVINASQKKVLL 550
[Close]
BLAST of GT627153 vs. TAIR peptide
Match: AT5G63770.1 (| Symbols: ATDGK2, DGK2 | diacylglycerol kinase 2 | chr5:25519293-25522404 FORWARD LENGTH=712)

HSP 1 Score: 317.39 bits (812), Expect = 5.561e-87
Identity = 155/258 (60.08%), Postives = 197/258 (76.36%), Query Frame = 1 
Query:    1 VERQGGLTTVLHHIEHAAVTILDRWKVTISNPQGKQ--QLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFPVQIDGEPWFQEPCTVSIAHHGQAFMLKRAAEEPLGHAAAMITDVLENAETNNVISASQKRALL 768
            V+ QG L T L  I+HAAVT+LDRW V I     ++    +  KFM NYLGIGCDAKVA + H +R+E PEKF +QF+NK+ YA+EGA+ IMDR   D PWQ+ + VDG +IE+P+D+EG++V NIGSYMGGVDLWQN+ E+ DNF  Q MHDK LEVV + G WHLGKLQVGLS+ARRLAQG+ I+I + + FPVQIDGEP+ Q+P  + I HHGQ FML+RA++EP GHAAA++ +VL +AE   VI+ASQK+ LL
Sbjct:  447 VDGQGSLRTFLQDIDHAAVTMLDRWSVKIVEESTEKFPAREGHKFMMNYLGIGCDAKVAYEFHMMRQEKPEKFCSQFVNKLRYAKEGARDIMDRACADLPWQVWLEVDGKDIEIPKDSEGLIVLNIGSYMGGVDLWQNDYEHDDNFSIQCMHDKTLEVVCVRGAWHLGKLQVGLSQARRLAQGKVIRIHVSSPFPVQIDGEPFIQQPGCLEITHHGQVFMLRRASDEPRGHAAAIMNEVLLDAECKGVINASQKKVLL 704
[Close]
BLAST of GT627153 vs. TAIR peptide
Match: AT5G57690.1 (| Symbols: ATDGK4, DGK4 | diacylglycerol kinase 4 | chr5:23372063-23374469 REVERSE LENGTH=487)

HSP 1 Score: 76.6406 bits (187), Expect = 1.652e-14
Identity = 63/256 (24.61%), Postives = 102/256 (39.84%), Query Frame = 1 
Query:   10 QGGLTTVLHHIEHAAVTILDRWKVTISNPQGK-----------------QQLQ-----PPK------FMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYA------------------REGAKSIMD---RTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENY---DNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQL----FAKFPVQIDGEPWFQ 609
            +  +   LH    A ++ LD W + I+ P G+                 Q L+     PP          NY  IG DA+VA   H+LR E P        NK++Y+                    G K+IM    +  +   W+        ++ VP+    V+  N+ SY  G + W N  ++Y     F      D +LE+  +   WH   + V L  A+ +AQ  +I++++    +    +Q+DGEPW Q
Sbjct:  215 KSAIKRTLHRASVAPISRLDSWNILITMPSGEIVDPPYSLKATQECYIDQNLEIEGEIPPSTNGYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGWFLTHCINDPGLRGLKNIMTLHIKKLDSSEWE--------KVPVPKSVRAVVALNLHSYGSGRNPWGNLKQDYLEKRGFVEAQADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWKDAFMQMDGEPWKQ 462
[Close]
BLAST of GT627153 vs. TAIR peptide
Match: AT2G18730.1 (| Symbols: ATDGK3, DGK3 | diacylglycerol kinase 3 | chr2:8118976-8121689 FORWARD LENGTH=488)

HSP 1 Score: 73.559 bits (179), Expect = 1.399e-13
Identity = 60/246 (24.39%), Postives = 94/246 (38.21%), Query Frame = 1 
Query:   31 LHHIEHAAVTILDRWKVTISNPQGKQQLQPPKFMN-----------------------------NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKS-------IMDRTFEDFPWQIRVVVDGV------EIEVPEDAEGVLVANIGSYMGGVDLWQNEDENY---DNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQL----FAKFPVQIDGEPWFQEPCT 621
            LH      V  LD WK+ +S P G + + PP  +                              NYL IG DA+VA   H+LR   P        NK++Y+  G          + D         +++ +  V      EI VP++   ++  N+ SY  G   W N   +Y     F      D ++E+      WH   +   L  A+ +AQ  +++ +L    +    +Q+DGEPW Q   T
Sbjct:  223 LHRASMGPVARLDSWKILVSMPSG-EVVDPPYSLKPAEENELDQGLDAGIDAPPLAKAYEGVFYNYLSIGMDAQVAYGFHHLRNTKPYLAQGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKKVNCSQWEEIAVPKNVRSIVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFGFKQGWHASFVMAELISAKHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMST 467
[Close]
BLAST of GT627153 vs. TAIR peptide
Match: AT4G30340.1 (| Symbols: ATDGK7, DGK7 | diacylglycerol kinase 7 | chr4:14838465-14840941 REVERSE LENGTH=492)

HSP 1 Score: 72.4034 bits (176), Expect = 3.116e-13
Identity = 63/257 (24.51%), Postives = 98/257 (38.13%), Query Frame = 1 
Query:   10 QGGLTTVLHHIEHAAVTILDRWKVTISNPQGK------------------QQLQ-----PPKFMN------NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLY------------------AREGAKSIMD---RTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENY---DNFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQL----FAKFPVQIDGEPWFQ 609
            +  +   LH     ++  LD WK+ +S P G+                  Q L      PPK  +      NY  IG DA+VA   H+LR E P        NK++Y                  A  G ++IM    +      W+        EI VP+    ++V N+ +Y  G   W N    Y     F      D ++E+  +   WH   +   +  A+ +AQ  +I+ +L    +    +Q+DGEPW Q
Sbjct:  219 RSAMKRTLHRATLGSIARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQALDADGDVPPKAKSYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYSSYSCTQGWFCTPCVNNPALRGLRNIMKIHIKKANCSEWE--------EIHVPKSVRSIVVLNLYNYGSGRHPWGNLRPKYLEKRGFVEAHCDDGLIEIFGLKQGWHASFVMAEIISAKHIAQAAAIRFELRGGDWKNAFLQMDGEPWKQ 467
[Close]
BLAST of GT627153 vs. TAIR peptide
Match: AT4G28130.1 (| Symbols: DGK6, ATDGK6 | diacylglycerol kinase 6 | chr4:13971552-13974323 FORWARD LENGTH=466)

HSP 1 Score: 71.633 bits (174), Expect = 5.315e-13
Identity = 52/198 (26.26%), Postives = 92/198 (46.46%), Query Frame = 1 
Query:  136 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGA------KSIMDRTFEDFPWQIRVVV-----DGVEIEVPEDAEGVLVANIGSYMGGVDLW--QNEDENYD-NFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQL---FAKFP-VQIDGEPWFQ------EPCTVSIAHHGQAFML 657
            NY  +G DA+V+   H+ R+ +PE+F NQ +N+  Y +          S+   + ++     ++ +        ++ +P+    ++  N+ S+ GG++ W   N  +  D +     + D ++E+V     WH   L        RLAQ   ++++     AK   ++IDGEPW Q      E   V I+HHGQ  ML
Sbjct:  235 NYFSLGMDAQVSYAFHSQRKLHPERFKNQLVNQSTYLKLSCTQGWFFASLFHPSSQNIAKLAKIQICDRNGQWNDLHIPQSIRSIVCLNLPSFSGGLNPWGTPNPKKQRDRSLTAPFVDDGLIEIVGFRNAWHGLILLSPNGHGTRLAQANRVRLEFKKGAAKHAYMRIDGEPWKQPLPSNDETVMVEISHHGQVNML 432
[Close]
BLAST of GT627153 vs. TAIR peptide
Match: AT2G20900.4 (| Symbols: DGK5, ATDGK5 | diacylglycerol kinase 5 | chr2:8989412-8992798 REVERSE LENGTH=509)

HSP 1 Score: 71.633 bits (174), Expect = 5.315e-13
Identity = 53/198 (26.77%), Postives = 86/198 (43.43%), Query Frame = 1 
Query:  136 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREG------AKSIMDRTFEDFPWQIRVVV-----DGVEIEVPEDAEGVLVANIGSYMGGVDLW--QNEDENYD-NFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFP----VQIDGEPWFQ------EPCTVSIAHHGQAFML 657
            NY  +G DA+++   H+ R+ +PEKF NQ +N+  Y + G        S+      +     +V +        ++ +P     ++  N+ S+ GG++ W   N  +  D    P  + D ++EVV     WH   L        RLAQ   I+ +          +++DGEPW Q      E   V I+H GQ  ML
Sbjct:  234 NYFSLGMDAQISYAFHSERKLHPEKFKNQLVNQSTYVKLGCTQGWFCASLFHPASRNIAQLAKVKIATRNGQWQDLHIPHSIRSIVCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHGTRLAQANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHLGQVNML 431
[Close]
BLAST of GT627153 vs. TAIR peptide
Match: AT2G20900.2 (| Symbols: DGK5, ATDGK5 | diacylglycerol kinase 5 | chr2:8989794-8992798 REVERSE LENGTH=491)

HSP 1 Score: 71.633 bits (174), Expect = 5.315e-13
Identity = 53/198 (26.77%), Postives = 86/198 (43.43%), Query Frame = 1 
Query:  136 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREG------AKSIMDRTFEDFPWQIRVVV-----DGVEIEVPEDAEGVLVANIGSYMGGVDLW--QNEDENYD-NFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFP----VQIDGEPWFQ------EPCTVSIAHHGQAFML 657
            NY  +G DA+++   H+ R+ +PEKF NQ +N+  Y + G        S+      +     +V +        ++ +P     ++  N+ S+ GG++ W   N  +  D    P  + D ++EVV     WH   L        RLAQ   I+ +          +++DGEPW Q      E   V I+H GQ  ML
Sbjct:  234 NYFSLGMDAQISYAFHSERKLHPEKFKNQLVNQSTYVKLGCTQGWFCASLFHPASRNIAQLAKVKIATRNGQWQDLHIPHSIRSIVCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHGTRLAQANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHLGQVNML 431
[Close]
BLAST of GT627153 vs. TAIR peptide
Match: AT2G20900.3 (| Symbols: DGK5, ATDGK5 | diacylglycerol kinase 5 | chr2:8989794-8992798 REVERSE LENGTH=491)

HSP 1 Score: 71.633 bits (174), Expect = 5.315e-13
Identity = 53/198 (26.77%), Postives = 86/198 (43.43%), Query Frame = 1 
Query:  136 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREG------AKSIMDRTFEDFPWQIRVVV-----DGVEIEVPEDAEGVLVANIGSYMGGVDLW--QNEDENYD-NFDPQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLFAKFP----VQIDGEPWFQ------EPCTVSIAHHGQAFML 657
            NY  +G DA+++   H+ R+ +PEKF NQ +N+  Y + G        S+      +     +V +        ++ +P     ++  N+ S+ GG++ W   N  +  D    P  + D ++EVV     WH   L        RLAQ   I+ +          +++DGEPW Q      E   V I+H GQ  ML
Sbjct:  234 NYFSLGMDAQISYAFHSERKLHPEKFKNQLVNQSTYVKLGCTQGWFCASLFHPASRNIAQLAKVKIATRNGQWQDLHIPHSIRSIVCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHGTRLAQANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHLGQVNML 431
The following BLAST results are available for this feature:
BLAST of GT627153 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
B9I2W0_POPTR5.111e-13089.06Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
D7SI39_VITVI4.327e-12989.11Whole genome shotgun sequence of line PN40024, sca... [more]
B9RLR5_RICCO5.651e-12987.50Diacylglycerol kinase, theta, putative OS=Ricinus ... [more]
A5BDX3_VITVI6.248e-12888.33Putative uncharacterized protein OS=Vitis vinifera... [more]
D7M0N5_ARALY2.374e-12785.94Putative uncharacterized protein OS=Arabidopsis ly... [more]
Q75GS1_ORYSJ9.359e-11678.52Putative diacylglycerol kinase (With alternative s... [more]
C7IZW2_ORYSJ9.359e-11678.52Os03g0425300 protein (Fragment) OS=Oryza sativa su... [more]
B9F945_ORYSJ9.359e-11678.52Putative uncharacterized protein OS=Oryza sativa s... [more]
B8AK66_ORYSI9.359e-11678.52Putative uncharacterized protein OS=Oryza sativa s... [more]
C5WWI1_SORBI6.066e-11578.52Putative uncharacterized protein Sb01g032250 OS=So... [more]
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BLAST of GT627153 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10
Match NameE-valueIdentityDescription
DGK1_ARATH4.504e-12885.55Diacylglycerol kinase 1 OS=Arabidopsis thaliana GN... [more]
DGKE_MOUSE2.901e-3438.01Diacylglycerol kinase epsilon OS=Mus musculus GN=D... [more]
DGKQ_MOUSE6.463e-3436.56Diacylglycerol kinase theta OS=Mus musculus GN=Dgk... [more]
DGKQ_HUMAN1.880e-3336.56Diacylglycerol kinase theta OS=Homo sapiens GN=DGK... [more]
DGKE_HUMAN3.207e-3337.56Diacylglycerol kinase epsilon OS=Homo sapiens GN=D... [more]
DGKG_RAT1.032e-3135.04Diacylglycerol kinase gamma OS=Rattus norvegicus G... [more]
DGKB_MOUSE1.032e-3134.87Diacylglycerol kinase beta OS=Mus musculus GN=Dgkb... [more]
DGKG_MOUSE1.348e-3135.04Diacylglycerol kinase gamma OS=Mus musculus GN=Dgk... [more]
DGKH_DROMO2.299e-3137.78Diacylglycerol kinase eta OS=Drosophila mojavensis... [more]
DGKB_RAT2.299e-3134.45Diacylglycerol kinase beta OS=Rattus norvegicus GN... [more]
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BLAST of GT627153 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 10
Match NameE-valueIdentityDescription
AT5G07920.14.497e-12985.55| Symbols: DGK1, ATDGK1 | diacylglycerol kinase1 |... [more]
AT5G63770.25.561e-8760.08| Symbols: ATDGK2, DGK2 | diacylglycerol kinase 2 ... [more]
AT5G63770.15.561e-8760.08| Symbols: ATDGK2, DGK2 | diacylglycerol kinase 2 ... [more]
AT5G57690.11.652e-1424.61| Symbols: ATDGK4, DGK4 | diacylglycerol kinase 4 ... [more]
AT2G18730.11.399e-1324.39| Symbols: ATDGK3, DGK3 | diacylglycerol kinase 3 ... [more]
AT4G30340.13.116e-1324.51| Symbols: ATDGK7, DGK7 | diacylglycerol kinase 7 ... [more]
AT4G28130.15.315e-1326.26| Symbols: DGK6, ATDGK6 | diacylglycerol kinase 6 ... [more]
AT2G20900.45.315e-1326.77| Symbols: DGK5, ATDGK5 | diacylglycerol kinase 5 ... [more]
AT2G20900.25.315e-1326.77| Symbols: DGK5, ATDGK5 | diacylglycerol kinase 5 ... [more]
AT2G20900.35.315e-1326.77| Symbols: DGK5, ATDGK5 | diacylglycerol kinase 5 ... [more]
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InterPro
Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000756Diacylglycerol kinase, accessory domainPFAMPF00609DAGK_acccoord: 43..200
score: 9.1
IPR000756Diacylglycerol kinase, accessory domainSMARTSM00045no descriptioncoord: 43..200
score: 6.7
NoneNo IPR availablePANTHERPTHR11255DIACYLGLYCEROL KINASEcoord: 1..245
score: 3.4e
NoneNo IPR availablePANTHERPTHR11255:SF8DIACYLGLYCEROL KINASE, THETAcoord: 1..245
score: 3.4e
NoneNo IPR availableSEGsegsegcoord: 1..16
score: NAcoord: 36..48
score
NoneNo IPR availableSEGsegsegcoord: 8..24
score

Properties
Property NameValue
Singlet in analysisLens culinaris unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GT627153 ID=GT627153; Name=GT627153; organism=Lens culinaris; type=EST; length=772bp
GTGGAGAGGCAAGGGGGTCTGACCACAGTTTTGCATCATATAGAACATGC
TGCGGTTACTATTCTTGACCGGTGGAAGGTAACAATCAGTAACCCACAAG
GAAAGCAACAACTACAACCACCAAAATTTATGAATAATTATCTTGGAATT
GGTTGTGATGCAAAGGTTGCTTTGGACATCCATAATCTGCGGGAGGAAAA
TCCAGAGAAGTTTTATAACCAGTTTATGAATAAAGTTCTGTATGCAAGAG
AAGGTGCTAAAAGCATAATGGATAGGACATTTGAGGATTTCCCTTGGCAG
ATTCGTGTTGTGGTAGATGGAGTTGAAATTGAGGTCCCTGAGGATGCTGA
AGGGGTTCTTGTTGCAAATATTGGAAGCTACATGGGAGGTGTAGATTTGT
GGCAAAATGAGGATGAGAATTATGATAATTTTGATCCACAATCTATGCAC
GACAAAATACTTGAAGTTGTGAGCATATCTGGAACATGGCACCTGGGGAA
GCTTCAGGTTGGGCTTTCTCGAGCTCGAAGACTTGCTCAAGGACAGTCAA
TTAAGATACAATTATTTGCTAAGTTTCCCGTTCAAATTGATGGAGAACCT
TGGTTTCAGGAACCCTGTACAGTTTCCATTGCTCATCACGGCCAGGCCTT
TATGTTGAAGAGGGCGGCTGAGGAACCTCTTGGTCATGCAGCTGCAATGA
TCACTGATGTGCTTGAGAATGCTGAAACCAATAATGTAATTAGTGCATCA
CAAAAGCGAGCCCTTCTTCATG
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004143ATP-dependent diacylglycerol kinase activity
Vocabulary: Biological Process
TermDefinition
GO:0007205protein kinase C-activating G protein-coupled receptor signaling pathway
Vocabulary: INTERPRO
TermDefinition
IPR000756Diacylglycerol_kin_accessory