GT620366

EST Overview


Name	GT620366
Unique Name	GT620366
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	745

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
KEGG analysis for Lens culinaris unigene v1	2011-01-05
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT620366 vs. TrEMBL
Match: C6SVE0_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 303.138 bits (775), Expect = 1.554e-80
Identity = 147/160 (91.88%), Postives = 150/160 (93.75%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPKN 539
            MSDEEHHF+       SKTYPQQAGTIRKNGYIVIK RPCKVVEVSTSKTGKHGHAKCHF AIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNG+TKDDLKLPTDEALL QIKDGF+EGKDLVVSVMSAMGEEQICSLKDIGPKN
Sbjct:    1 MSDEEHHFDSQADAGASKTYPQQAGTIRKNGYIVIKSRPCKVVEVSTSKTGKHGHAKCHFAAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLKLPTDEALLAQIKDGFSEGKDLVVSVMSAMGEEQICSLKDIGPKN 160

[Close]

BLAST of GT620366 vs. TrEMBL
Match: B7FGR9_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 303.138 bits (775), Expect = 1.554e-80
Identity = 147/159 (92.45%), Postives = 151/159 (94.97%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPK 536
            MSDEEHHFE       SKTYPQQAGTIRKNGYIVIK RPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTD++LLTQIKDGFA+GKDLVVSVMSAMGEEQIC+LKDIGPK
Sbjct:    1 MSDEEHHFEPAADAGASKTYPQQAGTIRKNGYIVIKSRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDDSLLTQIKDGFADGKDLVVSVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of GT620366 vs. TrEMBL
Match: C6ZHS4_SOYBN (Eukaryotic translation initiation factor 5A2 OS=Glycine max PE=2 SV=1)

HSP 1 Score: 298.901 bits (764), Expect = 2.930e-79
Identity = 144/160 (90.00%), Postives = 150/160 (93.75%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPKN 539
            MSDEEHHFE       SKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFV IDIF  KKLEDIVPSSHNCDVPHVNRTDYQLIDI+EDGF+SLLT+NG+TKDDLKLPTDE+LLTQIKDGFAEGKDLVVSVMSAMGEEQIC+LKDIGPKN
Sbjct:    1 MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDIAEDGFLSLLTENGNTKDDLKLPTDESLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 160

[Close]

BLAST of GT620366 vs. TrEMBL
Match: C7SCX7_POPDE (eIF5A2 OS=Populus deltoides PE=2 SV=1)

HSP 1 Score: 297.745 bits (761), Expect = 6.527e-79
Identity = 143/160 (89.38%), Postives = 149/160 (93.12%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPKN 539
            MSDEEHHFE       SKTYPQQAGTIRKNGYIVIK RPCKVVEVSTSKTGKHGHAKCHFV IDIFNGKKLEDIVPSSHNCDVPHVNR DYQLIDISEDGFVSLLT+NG+TKDDL+LPTD++LLTQIKDGF EGKDLVVSVMSAMGEEQIC+LKDIGPKN
Sbjct:    1 MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVGIDIFNGKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTENGNTKDDLRLPTDDSLLTQIKDGFGEGKDLVVSVMSAMGEEQICALKDIGPKN 160

[Close]

BLAST of GT620366 vs. TrEMBL
Match: A9PK40_9ROSI (Putative uncharacterized protein OS=Populus trichocarpa x Populus deltoides PE=2 SV=1)

HSP 1 Score: 297.745 bits (761), Expect = 6.527e-79
Identity = 143/160 (89.38%), Postives = 149/160 (93.12%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPKN 539
            MSDEEHHFE       SKTYPQQAGTIRKNGYIVIK RPCKVVEVSTSKTGKHGHAKCHFV IDIFNGKKLEDIVPSSHNCDVPHVNR DYQLIDISEDGFVSLLT+NG+TKDDL+LPTD++LLTQIKDGF EGKDLVVSVMSAMGEEQIC+LKDIGPKN
Sbjct:    1 MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVGIDIFNGKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTENGNTKDDLRLPTDDSLLTQIKDGFGEGKDLVVSVMSAMGEEQICALKDIGPKN 160

[Close]

BLAST of GT620366 vs. TrEMBL
Match: A9PB16_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_717121 PE=2 SV=1)

HSP 1 Score: 297.745 bits (761), Expect = 6.527e-79
Identity = 143/160 (89.38%), Postives = 149/160 (93.12%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPKN 539
            MSDEEHHFE       SKTYPQQAGTIRKNGYIVIK RPCKVVEVSTSKTGKHGHAKCHFV IDIFNGKKLEDIVPSSHNCDVPHVNR DYQLIDISEDGFVSLLT+NG+TKDDL+LPTD++LLTQIKDGF EGKDLVVSVMSAMGEEQIC+LKDIGPKN
Sbjct:    1 MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVGIDIFNGKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTENGNTKDDLRLPTDDSLLTQIKDGFGEGKDLVVSVMSAMGEEQICALKDIGPKN 160

[Close]

BLAST of GT620366 vs. TrEMBL
Match: A5B605_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_018888 PE=4 SV=1)

HSP 1 Score: 297.745 bits (761), Expect = 6.527e-79
Identity = 144/159 (90.57%), Postives = 149/159 (93.71%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPK 536
            MSDEEHHFE       SKTYPQQAGTIRKNGYIVIK RPCKVVEVSTSKTGKHGHAKCHFV IDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLT+NG+TKDDL+LPTD+ LLTQIKDGFAEGKDLVVSVMSAMGEEQIC+LKDIGPK
Sbjct:    1 MSDEEHHFESKGDAGASKTYPQQAGTIRKNGYIVIKSRPCKVVEVSTSKTGKHGHAKCHFVGIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDDNLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of GT620366 vs. TrEMBL
Match: Q71F50_HEVBR (Eukaryotic translation initiation factor 5A isoform I OS=Hevea brasiliensis GN=eIF-5A PE=2 SV=1)

HSP 1 Score: 296.204 bits (757), Expect = 1.899e-78
Identity = 143/160 (89.38%), Postives = 149/160 (93.12%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPKN 539
            MSDEEHHFE       SKTYPQQAGTIRKNGYIVIK RPCKVVEVSTSKTGKHGHAKCHFV IDIFN KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLT+NG+TKDDL+LPTDE LL+QIKDGFAEGKDLVV+VMSAMGEEQIC+LKDIGPKN
Sbjct:    1 MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVGIDIFNAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFAEGKDLVVTVMSAMGEEQICALKDIGPKN 160

[Close]

BLAST of GT620366 vs. TrEMBL
Match: C7SCX9_POPDE (eIF5A4 OS=Populus deltoides PE=2 SV=1)

HSP 1 Score: 296.204 bits (757), Expect = 1.899e-78
Identity = 142/160 (88.75%), Postives = 149/160 (93.12%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPKN 539
            MSDEEHHFE       SKTYPQQAGTIRKNGYIVIK RPCKVVEVSTSKTGKHGHAKCHFV IDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLT+NG+TKDDL+LPTD++LLT IKDGF EGKDLVV+VMSAMGEEQIC+LKDIGPKN
Sbjct:    1 MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVGIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDDSLLTPIKDGFGEGKDLVVTVMSAMGEEQICALKDIGPKN 160

[Close]

BLAST of GT620366 vs. TrEMBL
Match: B7FBH3_9ASPA (eIF-5A protein OS=Phalaenopsis hybrid cultivar GN=eifph1 PE=2 SV=1)

HSP 1 Score: 296.204 bits (757), Expect = 1.899e-78
Identity = 143/159 (89.94%), Postives = 150/159 (94.34%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPK 536
            MSDEEHHFE       SKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFN KKLEDIVPSSHNCDVPHVNRTDYQLIDI+EDGF+SLLT+NG+TKDDLKLPTD+ LLTQIKDGFAEGKDLVV+VMSAMGEEQIC+LKDIGPK
Sbjct:    1 MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNQKKLEDIVPSSHNCDVPHVNRTDYQLIDIAEDGFLSLLTENGNTKDDLKLPTDDNLLTQIKDGFAEGKDLVVTVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of GT620366 vs. SwissProt
Match: IF5A2_MEDSA (Eukaryotic translation initiation factor 5A-2 OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 303.138 bits (775), Expect = 1.022e-81
Identity = 147/159 (92.45%), Postives = 151/159 (94.97%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPK 536
            MSDEEHHFE       SKTYPQQAGTIRKNGYIVIK RPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTD++LLTQIKDGFA+GKDLVVSVMSAMGEEQIC+LKDIGPK
Sbjct:    1 MSDEEHHFEPAADAGASKTYPQQAGTIRKNGYIVIKSRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDDSLLTQIKDGFADGKDLVVSVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of GT620366 vs. SwissProt
Match: IF5A_MANES (Eukaryotic translation initiation factor 5A OS=Manihot esculenta PE=2 SV=2)

HSP 1 Score: 294.278 bits (752), Expect = 4.750e-79
Identity = 142/160 (88.75%), Postives = 149/160 (93.12%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPKN 539
            MSDEEHHFE       SKT+PQQAGTIRKNGYIVIK RPCKV+EVSTSKTGKHGHAKCHFV IDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLT+ G+TKDDL+LPTDE LL+QIKDGFAEGKDLVVSVMSAMGEE+ICSLKDIGPKN
Sbjct:    1 MSDEEHHFESKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVMEVSTSKTGKHGHAKCHFVGIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTETGNTKDDLRLPTDENLLSQIKDGFAEGKDLVVSVMSAMGEERICSLKDIGPKN 160

[Close]

BLAST of GT620366 vs. SwissProt
Match: IF5A2_NICPL (Eukaryotic translation initiation factor 5A-2 OS=Nicotiana plumbaginifolia GN=EIF-5A2 PE=2 SV=1)

HSP 1 Score: 294.278 bits (752), Expect = 4.750e-79
Identity = 143/159 (89.94%), Postives = 149/159 (93.71%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPK 536
            MSDEEH FE       SKTYPQQAGTIRKNG+IVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIF GKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLT+NG+TKDDL+LPTD+ LLTQIKDGFAEGKDLVVSVMSAMGEEQIC+LKDIGPK
Sbjct:    1 MSDEEHQFESKADAGASKTYPQQAGTIRKNGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDDNLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of GT620366 vs. SwissProt
Match: IF5A3_SOLTU (Eukaryotic translation initiation factor 5A-3 OS=Solanum tuberosum GN=EIF5A3 PE=2 SV=1)

HSP 1 Score: 291.197 bits (744), Expect = 4.021e-78
Identity = 141/160 (88.12%), Postives = 149/160 (93.12%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPKN 539
            MSDEEHHFE       SKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLT++G+TKDDL+LPTDE+LL Q+KDGF EGKDLVVSVMSAMGEEQI ++KDIG KN
Sbjct:    1 MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTESGNTKDDLRLPTDESLLKQVKDGFQEGKDLVVSVMSAMGEEQINAIKDIGTKN 160

[Close]

BLAST of GT620366 vs. SwissProt
Match: IF5A_SENVE (Eukaryotic translation initiation factor 5A OS=Senecio vernalis GN=EIFSV1 PE=2 SV=1)

HSP 1 Score: 290.812 bits (743), Expect = 5.251e-78
Identity = 142/159 (89.31%), Postives = 147/159 (92.45%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPK 536
            MSDEEH FE       SKTYPQQAGTIRK G+IVIK R CKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLT+NG+TKDDLKLPTD+ALLTQIKDGF EGKDLVVSVMSAMGEEQIC+LKDIGPK
Sbjct:    1 MSDEEHQFESKADAGASKTYPQQAGTIRKGGHIVIKNRACKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTDDALLTQIKDGFGEGKDLVVSVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of GT620366 vs. SwissProt
Match: IF5A4_SOLTU (Eukaryotic translation initiation factor 5A-4 OS=Solanum tuberosum GN=EIF5A4 PE=2 SV=1)

HSP 1 Score: 290.812 bits (743), Expect = 5.251e-78
Identity = 142/159 (89.31%), Postives = 149/159 (93.71%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPK 536
            MSDEEH FE       SKTYPQQAGTIRK+GYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIF GKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNG+TKDDL+LPTD++LL+QIKDGFAEGKDLVVSVMSAMGEEQI +LKDIGPK
Sbjct:    1 MSDEEHQFESKADAGASKTYPQQAGTIRKSGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTDDSLLSQIKDGFAEGKDLVVSVMSAMGEEQINALKDIGPK 159

[Close]

BLAST of GT620366 vs. SwissProt
Match: IF5A3_SOLLC (Eukaryotic translation initiation factor 5A-3 OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 290.041 bits (741), Expect = 8.958e-78
Identity = 143/159 (89.94%), Postives = 147/159 (92.45%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPK 536
            MSDEEH FE       SKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIF GKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNG+TKDDL+LPTDE LL+ IKDGFAEGKDLVVSVMSAMGEEQI +LKDIGPK
Sbjct:    1 MSDEEHQFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTDENLLSLIKDGFAEGKDLVVSVMSAMGEEQINALKDIGPK 159

[Close]

BLAST of GT620366 vs. SwissProt
Match: IF5A1_SOLTU (Eukaryotic translation initiation factor 5A-1/2 OS=Solanum tuberosum GN=EIF5A1 PE=2 SV=1)

HSP 1 Score: 290.041 bits (741), Expect = 8.958e-78
Identity = 139/160 (86.88%), Postives = 149/160 (93.12%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPKN 539
            MSDEEHHFE       SKTYPQQAGTIRK+GYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLT+NG+TKDDL+LPTD+ALL Q+K GF EGKDLV+SVMSAMGEEQIC++KDIG K+
Sbjct:    1 MSDEEHHFESKADAGASKTYPQQAGTIRKSGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDDALLNQVKGGFEEGKDLVLSVMSAMGEEQICAVKDIGTKS 160

[Close]

BLAST of GT620366 vs. SwissProt
Match: IF5A5_SOLTU (Eukaryotic translation initiation factor 5A-5 OS=Solanum tuberosum GN=EIF5A5 PE=2 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 1.528e-77
Identity = 138/159 (86.79%), Postives = 147/159 (92.45%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPK 536
            MSDEEHHFE       SKTYPQQAGTIRK+G+IVIK RPCKVVEVSTSKTGKHGHAKCHFVAIDIF GKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLT+NG+TKDDL+LPTD+ LL Q+KDGFAEGKDLV+SVMSAMGEEQIC +KDIGPK
Sbjct:    1 MSDEEHHFESKADAGASKTYPQQAGTIRKSGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDDTLLAQVKDGFAEGKDLVLSVMSAMGEEQICGIKDIGPK 159

[Close]

BLAST of GT620366 vs. SwissProt
Match: IF5A1_SOLLC (Eukaryotic translation initiation factor 5A-1 OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 288.886 bits (738), Expect = 1.996e-77
Identity = 138/159 (86.79%), Postives = 146/159 (91.82%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPK 536
            MSDEEHHFE       SKTYPQQAGTIRK G+IVIK RPCKVVEVSTSKTGKHGHAKCHFVAIDIF GKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLT+NG+TKDDL+LPTD+ LL Q+KDGFAEGKDLV+SVMSAMGEEQIC +KDIGPK
Sbjct:    1 MSDEEHHFESKADAGASKTYPQQAGTIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDDTLLAQVKDGFAEGKDLVLSVMSAMGEEQICGIKDIGPK 159

[Close]

BLAST of GT620366 vs. TAIR peptide
Match: AT1G69410.1 (| Symbols: ATELF5A-3, ELF5A-3 | eukaryotic elongation factor 5A-3 | chr1:26089301-26090194 FORWARD LENGTH=158)

HSP 1 Score: 282.337 bits (721), Expect = 1.858e-76
Identity = 137/159 (86.16%), Postives = 146/159 (91.82%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPK 536
            MSD+EHHFE       SKTYPQQAG IRK G+IVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIF  KKLEDIVPSSHNCDVPHVNR DYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQ+K+GF EGKD+VVSVMSAMGEEQ+C+LK++GPK
Sbjct:    1 MSDDEHHFESSDAGA-SKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQLKNGFEEGKDIVVSVMSAMGEEQMCALKEVGPK 158

[Close]

BLAST of GT620366 vs. TAIR peptide
Match: AT1G13950.1 (| Symbols: EIF-5A, ELF5A-1, ATELF5A-1, EIF5A | eukaryotic elongation factor 5A-1 | chr1:4773631-4774668 FORWARD LENGTH=158)

HSP 1 Score: 279.256 bits (713), Expect = 1.573e-75
Identity = 139/159 (87.42%), Postives = 143/159 (89.94%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIGPK 536
            MSDEEHHFE       SKTYPQQAGTIRKNGYIVIK RPCKVVEVSTSKTGKHGHAKCHFVAIDIF  KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDG+VSLLTDNGSTKDDLKLP D+ LL QIK GF +GKDLVVSVMSAMGEEQI +LKDIGPK
Sbjct:    1 MSDEEHHFESSDAGA-SKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQQIKSGFDDGKDLVVSVMSAMGEEQINALKDIGPK 158

[Close]

BLAST of GT620366 vs. TAIR peptide
Match: AT1G26630.1 (| Symbols: FBR12, ATELF5A-2, ELF5A-2 | Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein | chr1:9205968-9207098 FORWARD LENGTH=159)

HSP 1 Score: 257.299 bits (656), Expect = 6.400e-69
Identity = 124/157 (78.98%), Postives = 137/157 (87.26%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQIKDGFAEGKDLVVSVMSAMGEEQICSLKDIG 530
            MSD+EHHFE       SKTYPQ AG IRK G+IVIK RPCKVVEVSTSKTGKHGHAKCHFVAIDIF  KKLEDIVPSSHNCDVPHVNR DYQLIDI+EDGFVSLLTD+G TKDDLKLPTD+ L  Q++ GF EGKD+VVSVMS+MGEEQIC++K++G
Sbjct:    1 MSDDEHHFEASESGA-SKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQMRLGFDEGKDIVVSVMSSMGEEQICAVKEVG 156

[Close]

BLAST of GT620366 vs. TAIR peptide
Match: AT1G26630.2 (| Symbols: FBR12, ATELF5A-2, ELF5A-2 | Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein | chr1:9205968-9207013 FORWARD LENGTH=138)

HSP 1 Score: 213.386 bits (542), Expect = 1.060e-55
Identity = 103/127 (81.10%), Postives = 109/127 (85.83%), Query Frame = 3

Query:   60 MSDEEHHFEXXXXXXXSKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQI 440
            MSD+EHHFE       SKTYPQ AG IRK G+IVIK RPCKVVEVSTSKTGKHGHAKCHFVAIDIF  KKLEDIVPSSHNCDVPHVNR DYQLIDI+EDGFVSLLTD+G TKDDLKLPTD+ L  Q+
Sbjct:    1 MSDDEHHFEASESGA-SKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQL 126

The following BLAST results are available for this feature:

BLAST of GT620366 vs. TrEMBL
BLAST of GT620366 vs. SwissProt
BLAST of GT620366 vs. TAIR peptide

BLAST of GT620366 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6SVE0_SOYBN	1.554e-80	91.88	Putative uncharacterized protein OS=Glycine max PE...	[more]
B7FGR9_MEDTR	1.554e-80	92.45	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6ZHS4_SOYBN	2.930e-79	90.00	Eukaryotic translation initiation factor 5A2 OS=Gl...	[more]
C7SCX7_POPDE	6.527e-79	89.38	eIF5A2 OS=Populus deltoides PE=2 SV=1	[more]
A9PK40_9ROSI	6.527e-79	89.38	Putative uncharacterized protein OS=Populus tricho...	[more]
A9PB16_POPTR	6.527e-79	89.38	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A5B605_VITVI	6.527e-79	90.57	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q71F50_HEVBR	1.899e-78	89.38	Eukaryotic translation initiation factor 5A isofor...	[more]
C7SCX9_POPDE	1.899e-78	88.75	eIF5A4 OS=Populus deltoides PE=2 SV=1	[more]
B7FBH3_9ASPA	1.899e-78	89.94	eIF-5A protein OS=Phalaenopsis hybrid cultivar GN=...	[more]

BLAST of GT620366 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
IF5A2_MEDSA	1.022e-81	92.45	Eukaryotic translation initiation factor 5A-2 OS=M...	[more]
IF5A_MANES	4.750e-79	88.75	Eukaryotic translation initiation factor 5A OS=Man...	[more]
IF5A2_NICPL	4.750e-79	89.94	Eukaryotic translation initiation factor 5A-2 OS=N...	[more]
IF5A3_SOLTU	4.021e-78	88.13	Eukaryotic translation initiation factor 5A-3 OS=S...	[more]
IF5A_SENVE	5.251e-78	89.31	Eukaryotic translation initiation factor 5A OS=Sen...	[more]
IF5A4_SOLTU	5.251e-78	89.31	Eukaryotic translation initiation factor 5A-4 OS=S...	[more]
IF5A3_SOLLC	8.958e-78	89.94	Eukaryotic translation initiation factor 5A-3 OS=S...	[more]
IF5A1_SOLTU	8.958e-78	86.88	Eukaryotic translation initiation factor 5A-1/2 OS...	[more]
IF5A5_SOLTU	1.528e-77	86.79	Eukaryotic translation initiation factor 5A-5 OS=S...	[more]
IF5A1_SOLLC	1.996e-77	86.79	Eukaryotic translation initiation factor 5A-1 OS=S...	[more]

BLAST of GT620366 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT1G69410.1	1.858e-76	86.16	\| Symbols: ATELF5A-3, ELF5A-3 \| eukaryotic elongat...	[more]
AT1G13950.1	1.573e-75	87.42	\| Symbols: EIF-5A, ELF5A-1, ATELF5A-1, EIF5A \| euk...	[more]
AT1G26630.1	6.400e-69	78.98	\| Symbols: FBR12, ATELF5A-2, ELF5A-2 \| Eukaryotic ...	[more]
AT1G26630.2	1.060e-55	81.10	\| Symbols: FBR12, ATELF5A-2, ELF5A-2 \| Eukaryotic ...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 20..37 score
None	No IPR available	SEG	seg	seg	coord: 22..41 score
None	No IPR available	PANTHER	PTHR11673:SF2	INITIATION FACTOR 5A	coord: 1..166 score: 1.4e
None	No IPR available	SEG	seg	seg	coord: 17..23 score
None	No IPR available	SEG	seg	seg	coord: 18..32 score
None	No IPR available	SEG	seg	seg	coord: 64..76 score
IPR001884	Translation elongation factor IF5A	PIR	PIRSF003025	Translation initiation factor eIF-5A	coord: 8..165 score: 1.3e
IPR001884	Translation elongation factor IF5A	PANTHER	PTHR11673	TRANSLATION INITIATION FACTOR 5A-RELATED	coord: 1..166 score: 1.4e
IPR001884	Translation elongation factor IF5A	TIGRFAMs	TIGR00037	eIF_5A: translation initiation factor eIF-5A	coord: 23..161 score: 6.2
IPR005824	KOW	PFAM	PF00467	KOW	coord: 36..56 score: 1
IPR008991	Translation protein SH3-like	SUPERFAMILY	50104	Translation proteins SH3-like domain	coord: 21..100 score: 1.9
IPR012340	Nucleic acid-binding, OB-fold	GENE3D	2.40.50.140	no description	coord: 97..164 score: 3.5
IPR014722	Translation protein SH3-like, subgroup	GENE3D	2.30.30.30	no description	coord: 21..96 score: 1.7
IPR016027	Nucleic acid-binding, OB-fold-like	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 93..164 score: 6.5
IPR019769	Translation elongation factor, IF5A, hypusine site	PROSITE	PS00302	IF5A_HYPUSINE	coord: 57..64 score
IPR020189	Translation elongation factor, IF5A C-terminal	PFAM	PF01287	eIF-5a	coord: 92..161 score: 6.8

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT620366 ID=GT620366; Name=GT620366; organism=Lens culinaris; type=EST; length=745bp
TTCTTCTTCTTCAACTCCTCGAACGTTTCGCACACTGAAGAGAAGAGAAT
TCATCAACCATGTCCGACGAAGAGCACCACTTCGAAGCTGCCGCCGACGC
CGGAGCCTCCAAGACTTATCCTCAGCAGGCCGGTACCATTCGCAAGAACG
GTTACATCGTCATCAAGGGCAGGCCATGCAAGGTTGTTGAAGTTTCTACT
TCAAAAACAGGAAAGCATGGACATGCAAAGTGTCACTTTGTTGCAATTGA
TATTTTCAATGGGAAAAAGCTTGAAGATATTGTTCCTTCATCTCACAATT
GTGATGTTCCCCATGTGAACCGTACTGACTATCAGCTGATTGATATTTCT
GAAGATGGTTTTGTGAGTCTGTTGACTGATAATGGAAGTACTAAGGATGA
TTTGAAACTTCCCACTGATGAAGCACTCCTTACTCAGATTAAAGATGGAT
TTGCTGAAGGAAAAGATCTCGTGGTTTCTGTCATGTCTGCAATGGGAGAA
GAGCAGATATGTTCTTTGAAGGACATTGGTCCTAAGAATTAAAGTCATAT
ACTCTGGCTGTTGGGTGATAGCACATATTTTATTCTTATGTTGCTAACAG
GTAGTAATTTTTCATATGCATGTGTGCGCAATAAACTTGAAAAGATGGAA
GCTGCCTTGAGCTTGAGGCGGGAAATAGGAACTTTATTTATCTTTTGTCT
CTTAAGTTATTAAACCCTGCAGTTTTGGCTGGTTGAATTTGTCAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003723	RNA binding
GO:0003746	translation elongation factor activity
GO:0043022	ribosome binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006452	translational frameshifting
GO:0008612	peptidyl-lysine modification to peptidyl-hypusine
GO:0045901	positive regulation of translational elongation
GO:0045905	positive regulation of translational termination

Vocabulary: **INTERPRO**
Term	Definition
IPR001884	IF5A-like
IPR005824	KOW
IPR008991	Translation_prot_SH3-like_sf
IPR012340	NA-bd_OB-fold
IPR014722	Rib_uL2_dom2
IPR016027	Nucleic acid-binding, OB-fold-like
IPR019769	Trans_elong_IF5A_hypusine_site
IPR020189	Transl_elong_IF5A_C

Search form