AM161884

EST Overview
NameAM161884
Unique NameAM161884
TypeEST
OrganismPisum sativum (pea)
Sequence length450
Libraries
Library NameType
LIBEST_019360 Pisum sativum leaf vegetativecdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Homology
[Close]
BLAST of AM161884 vs. TrEMBL
Match: Q53YK5_ARATH (Peroxisomal sarcosine/pipecolate oxidase OS=Arabidopsis thaliana GN=At2g24580 PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.939e-23
Identity = 42/54 (77.78%), Postives = 47/54 (87.04%), Query Frame = -3 
Query:  218 LVDSTEPVMKQSCMYSMTPDEDFVIDFLGGEFGKNVVLGAGFSGHGFKMAPVIG 379
            +VDS  PV  Q CMYSMTPDEDFVIDFLGGEFG++VV+G GFSGHGFKMAP +G
Sbjct:  313 MVDSEGPVATQLCMYSMTPDEDFVIDFLGGEFGRDVVVGGGFSGHGFKMAPAVG 366

HSP 2 Score: 38.5058 bits (88), Expect = 1.939e-23
Identity = 14/21 (66.67%), Postives = 18/21 (85.71%), Query Frame = -1 
Query:  388 KRPWGSGVMMDEMKEWIEGRF 450
            KRPWG GV ++E+KEWI+ RF
Sbjct:  290 KRPWGPGVKLEELKEWIKERF 310
[Close]
BLAST of AM161884 vs. TrEMBL
Match: B9N674_POPTR (Sarcosine oxidase OS=Populus trichocarpa GN=SOX2 PE=4 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 6.292e-19
Identity = 44/54 (81.48%), Postives = 48/54 (88.89%), Query Frame = -3 
Query:  218 LVDSTEPVMKQSCMYSMTPDEDFVIDFLGGEFGKNVVLGAGFSGHGFKMAPVIG 379
            LVD   PV  QSCMYSMTPDEDFV+DFLGGEFGK+VV+G GFSGHGFKMAPV+G
Sbjct:  312 LVDYGGPVATQSCMYSMTPDEDFVLDFLGGEFGKDVVIGGGFSGHGFKMAPVVG 365
[Close]
BLAST of AM161884 vs. TrEMBL
Match: B9TA32_RICCO (Sarcosine oxidase, putative OS=Ricinus communis GN=RCOM_2005550 PE=4 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.073e-18
Identity = 45/54 (83.33%), Postives = 48/54 (88.89%), Query Frame = -3 
Query:  218 LVDSTEPVMKQSCMYSMTPDEDFVIDFLGGEFGKNVVLGAGFSGHGFKMAPVIG 379
            LVDS  PV  QSCMYSMTPDED+VIDFLG EFGK+VV+G GFSGHGFKMAPVIG
Sbjct:  311 LVDSDGPVATQSCMYSMTPDEDYVIDFLGEEFGKDVVVGGGFSGHGFKMAPVIG 364
[Close]
BLAST of AM161884 vs. TrEMBL
Match: Q2QP28_ORYSJ (Sarcosine oxidase, putative, expressed OS=Oryza sativa subsp. japonica GN=LOC_Os12g35890 PE=4 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 9.086e-18
Identity = 39/48 (81.25%), Postives = 46/48 (95.83%), Query Frame = -3 
Query:  218 PVMKQSCMYSMTPDEDFVIDFLGGEFGKNVVLGAGFSGHGFKMAPVIG 361
            PV++QSCMYSMTPDEDF+IDF+GGEFGK+VV+GAGFSGHGFKM P +G
Sbjct:  328 PVIRQSCMYSMTPDEDFIIDFVGGEFGKDVVVGAGFSGHGFKMGPAVG 375
[Close]
BLAST of AM161884 vs. TrEMBL
Match: Q0IMT5_ORYSJ (Os12g0543600 protein OS=Oryza sativa subsp. japonica GN=Os12g0543600 PE=4 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 9.086e-18
Identity = 39/48 (81.25%), Postives = 46/48 (95.83%), Query Frame = -3 
Query:  218 PVMKQSCMYSMTPDEDFVIDFLGGEFGKNVVLGAGFSGHGFKMAPVIG 361
            PV++QSCMYSMTPDEDF+IDF+GGEFGK+VV+GAGFSGHGFKM P +G
Sbjct:  328 PVIRQSCMYSMTPDEDFIIDFVGGEFGKDVVVGAGFSGHGFKMGPAVG 375
[Close]
BLAST of AM161884 vs. TrEMBL
Match: A3CI64_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_36399 PE=4 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 9.086e-18
Identity = 39/48 (81.25%), Postives = 46/48 (95.83%), Query Frame = -3 
Query:  218 PVMKQSCMYSMTPDEDFVIDFLGGEFGKNVVLGAGFSGHGFKMAPVIG 361
            PV++QSCMYSMTPDEDF+IDF+GGEFGK+VV+GAGFSGHGFKM P +G
Sbjct:  308 PVIRQSCMYSMTPDEDFIIDFVGGEFGKDVVVGAGFSGHGFKMGPAVG 355
[Close]
BLAST of AM161884 vs. TrEMBL
Match: A2ZLC9_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_38628 PE=4 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 9.086e-18
Identity = 39/48 (81.25%), Postives = 46/48 (95.83%), Query Frame = -3 
Query:  218 PVMKQSCMYSMTPDEDFVIDFLGGEFGKNVVLGAGFSGHGFKMAPVIG 361
            PV++QSCMYSMTPDEDF+IDF+GGEFGK+VV+GAGFSGHGFKM P +G
Sbjct:  328 PVIRQSCMYSMTPDEDFIIDFVGGEFGKDVVVGAGFSGHGFKMGPAVG 375
[Close]
BLAST of AM161884 vs. TrEMBL
Match: B9ILE2_POPTR (Sarcosine oxidase OS=Populus trichocarpa GN=SOX1 PE=4 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.550e-17
Identity = 43/54 (79.63%), Postives = 46/54 (85.19%), Query Frame = -3 
Query:  218 LVDSTEPVMKQSCMYSMTPDEDFVIDFLGGEFGKNVVLGAGFSGHGFKMAPVIG 379
            LVD   PV  Q CMYSMTPD DFVIDFLGGEFGK+VV+G GFSGHGFKMAPV+G
Sbjct:  311 LVDYGGPVATQLCMYSMTPDGDFVIDFLGGEFGKDVVVGGGFSGHGFKMAPVVG 364

HSP 2 Score: 60.4622 bits (145), Expect = 6.496e-8
Identity = 41/109 (37.61%), Postives = 57/109 (52.29%), Query Frame = -1 
Query:  127 KRPWGSGVMMDEMKEWIEGRFCWRWLIQPSL**NSLVCIQ*HRMRIL*LISWV-VSLGRMWFWVQGFRVTGLRWLRLLGKILTELAVGGETNEVDLKHFRIGRFNVSSK 450
            KRPWG G+  D MKEWIEGRF    L+       + +C+         +I ++    G+      GF   G +   ++G+IL +LA+ GE   VDLKHFRI RF  + K
Sbjct:  288 KRPWGPGISSDSMKEWIEGRF--SGLVDYGGPVATQLCMYSMTPDGDFVIDFLGGEFGKDVVVGGGFSGHGFKMAPVVGRILADLALSGEAKGVDLKHFRIQRFQENPK 394
[Close]
BLAST of AM161884 vs. TrEMBL
Match: D7LIA0_ARALY (Sarcosine oxidase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_481314 PE=4 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 2.024e-17
Identity = 41/54 (75.93%), Postives = 46/54 (85.19%), Query Frame = -3 
Query:  218 LVDSTEPVMKQSCMYSMTPDEDFVIDFLGGEFGKNVVLGAGFSGHGFKMAPVIG 379
            +VDS  PV  Q CMYSMTPDEDFVIDFLGGE G++VV+G GFSGHGFKMAP +G
Sbjct:  313 MVDSEGPVATQLCMYSMTPDEDFVIDFLGGELGRDVVVGGGFSGHGFKMAPAVG 366
[Close]
BLAST of AM161884 vs. TrEMBL
Match: B6TF50_MAIZE (Sarcosine oxidase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.453e-17
Identity = 40/48 (83.33%), Postives = 45/48 (93.75%), Query Frame = -3 
Query:  218 PVMKQSCMYSMTPDEDFVIDFLGGEFGKNVVLGAGFSGHGFKMAPVIG 361
            PV + SCMYSMTPD+DFVIDFLGGEFGK+VV+GAGFSGHGFKMAP +G
Sbjct:  326 PVTRLSCMYSMTPDKDFVIDFLGGEFGKDVVVGAGFSGHGFKMAPTVG 373
[Close]
BLAST of AM161884 vs. SwissProt
Match: SOX_ARATH (Probable sarcosine oxidase OS=Arabidopsis thaliana GN=At2g24580 PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.144e-24
Identity = 42/54 (77.78%), Postives = 47/54 (87.04%), Query Frame = -3 
Query:  218 LVDSTEPVMKQSCMYSMTPDEDFVIDFLGGEFGKNVVLGAGFSGHGFKMAPVIG 379
            +VDS  PV  Q CMYSMTPDEDFVIDFLGGEFG++VV+G GFSGHGFKMAP +G
Sbjct:  313 MVDSEGPVATQLCMYSMTPDEDFVIDFLGGEFGRDVVVGGGFSGHGFKMAPAVG 366

HSP 2 Score: 38.5058 bits (88), Expect = 1.144e-24
Identity = 14/21 (66.67%), Postives = 18/21 (85.71%), Query Frame = -1 
Query:  388 KRPWGSGVMMDEMKEWIEGRF 450
            KRPWG GV ++E+KEWI+ RF
Sbjct:  290 KRPWGPGVKLEELKEWIKERF 310
[Close]
BLAST of AM161884 vs. SwissProt
Match: SOX_RABIT (Peroxisomal sarcosine oxidase OS=Oryctolagus cuniculus GN=PIPOX PE=1 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.326e-9
Identity = 28/49 (57.14%), Postives = 37/49 (75.51%), Query Frame = -3 
Query:  218 EPVMKQSCMYSMTPDEDFVIDFLGGEFGKNVVLGAGFSGHGFKMAPVIG 364
            EP + +SCMY+ TPDE F++D        N+V+GAGFSGHGFK+APV+G
Sbjct:  312 EPAVIESCMYTNTPDEQFILD--RHPKYDNIVIGAGFSGHGFKLAPVVG 358
[Close]
BLAST of AM161884 vs. SwissProt
Match: SOX_HUMAN (Peroxisomal sarcosine oxidase OS=Homo sapiens GN=PIPOX PE=2 SV=2)

HSP 1 Score: 62.003 bits (149), Expect = 1.326e-9
Identity = 28/49 (57.14%), Postives = 37/49 (75.51%), Query Frame = -3 
Query:  218 EPVMKQSCMYSMTPDEDFVIDFLGGEFGKNVVLGAGFSGHGFKMAPVIG 364
            EP + +SCMY+ TPDE F++D        N+V+GAGFSGHGFK+APV+G
Sbjct:  312 EPAVIESCMYTNTPDEQFILD--RHPKYDNIVIGAGFSGHGFKLAPVVG 358
[Close]
BLAST of AM161884 vs. SwissProt
Match: SOX_MOUSE (Peroxisomal sarcosine oxidase OS=Mus musculus GN=Pipox PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 8.593e-9
Identity = 27/49 (55.10%), Postives = 36/49 (73.47%), Query Frame = -3 
Query:  218 EPVMKQSCMYSMTPDEDFVIDFLGGEFGKNVVLGAGFSGHGFKMAPVIG 364
            EP + + CMY+ TPDE F++D        N+V+GAGFSGHGFK+APV+G
Sbjct:  312 EPDIMERCMYTNTPDEHFILDCHPKY--DNIVIGAGFSGHGFKLAPVVG 358
[Close]
BLAST of AM161884 vs. SwissProt
Match: SOX_BOVIN (Peroxisomal sarcosine oxidase OS=Bos taurus GN=PIPOX PE=2 SV=2)

HSP 1 Score: 56.9954 bits (136), Expect = 4.265e-8
Identity = 26/49 (53.06%), Postives = 35/49 (71.43%), Query Frame = -3 
Query:  218 EPVMKQSCMYSMTPDEDFVIDFLGGEFGKNVVLGAGFSGHGFKMAPVIG 364
            EP + + CMY+ TPD  FV+D        N+V+GAGFSGHGFK++PV+G
Sbjct:  314 EPAVMEHCMYTNTPDGHFVLD--RHPKYDNIVIGAGFSGHGFKLSPVVG 360
[Close]
BLAST of AM161884 vs. TAIR peptide
Match: AT2G24580.1 (| Symbols: | FAD-dependent oxidoreductase family protein | chr2:10444928-10446178 REVERSE LENGTH=416)

HSP 1 Score: 94.3597 bits (233), Expect = 1.349e-25
Identity = 42/54 (77.78%), Postives = 47/54 (87.04%), Query Frame = -3 
Query:  218 LVDSTEPVMKQSCMYSMTPDEDFVIDFLGGEFGKNVVLGAGFSGHGFKMAPVIG 379
            +VDS  PV  Q CMYSMTPDEDFVIDFLGGEFG++VV+G GFSGHGFKMAP +G
Sbjct:  313 MVDSEGPVATQLCMYSMTPDEDFVIDFLGGEFGRDVVVGGGFSGHGFKMAPAVG 366

HSP 2 Score: 38.5058 bits (88), Expect = 1.349e-25
Identity = 14/21 (66.67%), Postives = 18/21 (85.71%), Query Frame = -1 
Query:  388 KRPWGSGVMMDEMKEWIEGRF 450
            KRPWG GV ++E+KEWI+ RF
Sbjct:  290 KRPWGPGVKLEELKEWIKERF 310
The following BLAST results are available for this feature:
BLAST of AM161884 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q53YK5_ARATH1.939e-2377.78Peroxisomal sarcosine/pipecolate oxidase OS=Arabid... [more]
B9N674_POPTR6.292e-1981.48Sarcosine oxidase OS=Populus trichocarpa GN=SOX2 P... [more]
B9TA32_RICCO1.073e-1883.33Sarcosine oxidase, putative OS=Ricinus communis GN... [more]
Q2QP28_ORYSJ9.086e-1881.25Sarcosine oxidase, putative, expressed OS=Oryza sa... [more]
Q0IMT5_ORYSJ9.086e-1881.25Os12g0543600 protein OS=Oryza sativa subsp. japoni... [more]
A3CI64_ORYSJ9.086e-1881.25Putative uncharacterized protein OS=Oryza sativa s... [more]
A2ZLC9_ORYSI9.086e-1881.25Putative uncharacterized protein OS=Oryza sativa s... [more]
B9ILE2_POPTR1.550e-1779.63Sarcosine oxidase OS=Populus trichocarpa GN=SOX1 P... [more]
D7LIA0_ARALY2.024e-1775.93Sarcosine oxidase family protein OS=Arabidopsis ly... [more]
B6TF50_MAIZE3.453e-1783.33Sarcosine oxidase OS=Zea mays PE=2 SV=1[more]
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BLAST of AM161884 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 5
Match NameE-valueIdentityDescription
SOX_ARATH1.144e-2477.78Probable sarcosine oxidase OS=Arabidopsis thaliana... [more]
SOX_RABIT1.326e-957.14Peroxisomal sarcosine oxidase OS=Oryctolagus cunic... [more]
SOX_HUMAN1.326e-957.14Peroxisomal sarcosine oxidase OS=Homo sapiens GN=P... [more]
SOX_MOUSE8.593e-955.10Peroxisomal sarcosine oxidase OS=Mus musculus GN=P... [more]
SOX_BOVIN4.265e-853.06Peroxisomal sarcosine oxidase OS=Bos taurus GN=PIP... [more]
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BLAST of AM161884 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT2G24580.11.349e-2577.78| Symbols: | FAD-dependent oxidoreductase family ... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 12..25
score: NAcoord: 49..62
score
NoneNo IPR availableGENE3D3.50.50.60no descriptioncoord: 13..56
score: 0.0
NoneNo IPR availableGENE3D3.50.50.60no descriptioncoord: 28..60
score: 1.9
NoneNo IPR availablePANTHERPTHR10961SARCOSINE OXIDASEcoord: 1..60
score: 8.1
IPR006076FAD dependent oxidoreductasePFAMPF01266DAOcoord: 10..60
score: 4.1

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>AM161884 ID=AM161884; Name=AM161884; organism=Pisum sativum; type=EST; length=450bp
ACTAGAGATTACAATCACTCTCAATACTAAACAAAATATTAGATCTACAA
AACAATAGTAAAAAGTCTTCAAACATCCTCGTCTTCAAACATCCTCTGTA
AGCATTCTTAACCTTCTCACCTAAAGCTTGGAACTCACATTGAATCTTCC
AATCCTAAAATGCTTCAAATCAACTTCATTAGTTTCCCCTCCCACCGCAA
GCTCAGTCAATATCTTACCCAATAACCGGAGCCATCTTAAACCCGTGACC
CGAAAACCCTGCACCCAAAACCACATTCTTCCCAAACTCACCACCCAAGA
AATCAATCACAAAATCCTCATCCGGTGTCATTGAATACATACAAGACTGT
TTCATCACAGGCTCGGTTGAATCAACCAACGCCAGCAAAATCTCCCTTCA
ATCCACTCTTTCATCTCATCCATCATCACACCAGAACCCCAGGGTCTCTT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: INTERPRO
TermDefinition
IPR006076FAD-dep_OxRdtase