Genetic control and QTL analysis of cotyledon bleaching resistance in green field pea (Pisum sativum L.)

Publication Overview
TitleGenetic control and QTL analysis of cotyledon bleaching resistance in green field pea (Pisum sativum L.)
AuthorsUbayasena L, Bett K, Tar'an B, Vijayan P, Warkentin T
TypeJournal Article
Journal NameGenome / National Research Council Canada = Génome / Conseil national de recherches Canada
Volume53
Issue5
Year2010
Page(s)346-59
CitationUbayasena L, Bett K, Tar'an B, Vijayan P, Warkentin T. Genetic control and QTL analysis of cotyledon bleaching resistance in green field pea (Pisum sativum L.). Genome / National Research Council Canada = Génome / Conseil national de recherches Canada. 2010 May; 53(5):346-59.

Abstract

Resistance to bleaching is an important factor for quality grading of Canadian green field pea and an important selection criterion in green pea improvement. This research was conducted to determine the genetic control of bleaching resistance in green peas using 90 recombinant inbred lines (RILs) derived from a cross between cultivars Orb and CDC Striker. These lines were evaluated under field conditions for two years in two locations in Saskatchewan, Canada. Harvested whole seeds and cotyledons were evaluated for greenness using the Hunter Lab colorimeter before and after exposure to a high light intensity accelerated bleaching treatment. The RILs were genotyped using amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) molecular markers. Heritability estimates for whole seed and cotyledon greenness were moderate (0.72 and 0.69, respectively) and increased when assessed after exposing whole seeds and cotyledons to accelerated bleaching conditions (0.83 and 0.82, respectively). The genetic linkage map constructed based on a total of 224 AFLP and SSR markers spanned over 890 cM of the pea genome. Multiple QTL mapping detected major QTLs on LG IV and LG V as well as location- and year-specific QTLs on LG II and LG III associated with green cotyledon bleaching resistance in field pea. The results demonstrated the importance of the seed coat in protecting the cotyledons from bleaching.

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Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Pea-Cotyledon_bleaching_resistance-Ubayasena-2010
Featuremaps
This publication contains information about 1 maps:
Map Name
pea-OrbxCDCStriker-RIL
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint
ISSN1480-3321
eISSN1480-3321
Publication Date2010 May
Journal AbbreviationGenome
DOI10.1139/g10-013
Elocation10.1139/g10-013
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryCanada
Publication TypeResearch Support, Non-U.S. Gov't
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PubMedPMID:20616866
AGL: USDA National Agricultural LibraryAGL:4385788