HO064583

EST Overview


Name	HO064583
Unique Name	HO064583
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	343

Libraries


Library Name	Type
LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO064583 vs. SwissProt
Match: COPG2_ORYSJ (Coatomer subunit gamma-2 OS=Oryza sativa subsp. japonica GN=Os07g0201100 PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 5.912e-24
Identity = 53/64 (82.81%), Postives = 58/64 (90.62%), Query Frame = -1

Query:  146 GTEVVPPNSRSHTCLLSGVYIGNVKVLVRLSFGLDGPKDVAMKLTVRSDDETVSDAIHEIVASG 337
            GT+VVP NSRSHTCLLSGV+IGNVKVLVRLSFGL GPK+VAMKL VRSDD  +SD IHEIVA+G
Sbjct:  826 GTDVVPSNSRSHTCLLSGVFIGNVKVLVRLSFGLSGPKEVAMKLAVRSDDPEISDKIHEIVANG 889

[Close]

BLAST of HO064583 vs. SwissProt
Match: COPG1_ORYSJ (Coatomer subunit gamma-1 OS=Oryza sativa subsp. japonica GN=Os03g0227000 PE=2 SV=2)

HSP 1 Score: 107.457 bits (267), Expect = 2.247e-23
Identity = 53/64 (82.81%), Postives = 57/64 (89.06%), Query Frame = -1

Query:  146 GTEVVPPNSRSHTCLLSGVYIGNVKVLVRLSFGLDGPKDVAMKLTVRSDDETVSDAIHEIVASG 337
            GTEVVP N+RSHTCLLSGV+IG+ KVLVRLSFGL GPK+VAMKL VRSDD  VSD IHEIVASG
Sbjct:  821 GTEVVPKNARSHTCLLSGVFIGDAKVLVRLSFGLSGPKEVAMKLAVRSDDPEVSDKIHEIVASG 884

[Close]

BLAST of HO064583 vs. SwissProt
Match: COPG_ARATH (Coatomer subunit gamma OS=Arabidopsis thaliana GN=At4g34450 PE=1 SV=2)

HSP 1 Score: 102.449 bits (254), Expect = 7.227e-22
Identity = 48/64 (75.00%), Postives = 57/64 (89.06%), Query Frame = -1

Query:  146 GTEVVPPNSRSHTCLLSGVYIGNVKVLVRLSFGLDGPKDVAMKLTVRSDDETVSDAIHEIVASG 337
            GTE +P N+RSHTCLLSGVYIGNVKVLVR  FG+D  KD+AMKLTVR++D +V++AIHEIVASG
Sbjct:  823 GTETIPLNARSHTCLLSGVYIGNVKVLVRAQFGMDSSKDIAMKLTVRAEDVSVAEAIHEIVASG 886

[Close]

BLAST of HO064583 vs. TrEMBL
Match: D7TQ06_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_63.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00031955001 PE=4 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 1.298e-24
Identity = 56/64 (87.50%), Postives = 62/64 (96.88%), Query Frame = -1

Query:  146 GTEVVPPNSRSHTCLLSGVYIGNVKVLVRLSFGLDGPKDVAMKLTVRSDDETVSDAIHEIVASG 337
            GTEVVP NSRSHTCLLSGV+IGN+KVLVRLSFG+DGPK+VAMKL VRS+DE+VSDAIHEIVASG
Sbjct:  824 GTEVVPSNSRSHTCLLSGVFIGNMKVLVRLSFGIDGPKEVAMKLAVRSEDESVSDAIHEIVASG 887

[Close]

BLAST of HO064583 vs. TrEMBL
Match: B9RBE3_RICCO (Coatomer gamma subunit, putative OS=Ricinus communis GN=RCOM_1674930 PE=4 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.099e-23
Identity = 54/64 (84.38%), Postives = 61/64 (95.31%), Query Frame = -1

Query:  146 GTEVVPPNSRSHTCLLSGVYIGNVKVLVRLSFGLDGPKDVAMKLTVRSDDETVSDAIHEIVASG 337
            GTEVVP NSRSHTC+LSGV+IGNVKVLV+L FG+DGPK+VAMKL VRS+DE+VSDAIHEIVASG
Sbjct:  824 GTEVVPSNSRSHTCVLSGVFIGNVKVLVQLQFGIDGPKEVAMKLAVRSEDESVSDAIHEIVASG 887

[Close]

BLAST of HO064583 vs. TrEMBL
Match: B9FW22_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_23473 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.215e-22
Identity = 53/64 (82.81%), Postives = 58/64 (90.62%), Query Frame = -1

Query:  146 GTEVVPPNSRSHTCLLSGVYIGNVKVLVRLSFGLDGPKDVAMKLTVRSDDETVSDAIHEIVASG 337
            GT+VVP NSRSHTCLLSGV+IGNVKVLVRLSFGL GPK+VAMKL VRSDD  +SD IHEIVA+G
Sbjct:  826 GTDVVPSNSRSHTCLLSGVFIGNVKVLVRLSFGLSGPKEVAMKLAVRSDDPEISDKIHEIVANG 889

[Close]

BLAST of HO064583 vs. TrEMBL
Match: B8B874_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_25286 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.215e-22
Identity = 53/64 (82.81%), Postives = 58/64 (90.62%), Query Frame = -1

Query:  146 GTEVVPPNSRSHTCLLSGVYIGNVKVLVRLSFGLDGPKDVAMKLTVRSDDETVSDAIHEIVASG 337
            GT+VVP NSRSHTCLLSGV+IGNVKVLVRLSFGL GPK+VAMKL VRSDD  +SD IHEIVA+G
Sbjct:  826 GTDVVPSNSRSHTCLLSGVFIGNVKVLVRLSFGLSGPKEVAMKLAVRSDDPEISDKIHEIVANG 889

[Close]

BLAST of HO064583 vs. TrEMBL
Match: B7ZZX2_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 3.536e-22
Identity = 53/64 (82.81%), Postives = 57/64 (89.06%), Query Frame = -1

Query:  146 GTEVVPPNSRSHTCLLSGVYIGNVKVLVRLSFGLDGPKDVAMKLTVRSDDETVSDAIHEIVASG 337
            GTEVVP N+RSHTCLLSGV+IGN+KVLVRLSFGL GP +VAMKL VRSDD  VSD IHEIVASG
Sbjct:  582 GTEVVPKNARSHTCLLSGVFIGNMKVLVRLSFGLSGPNEVAMKLAVRSDDPEVSDKIHEIVASG 645

[Close]

BLAST of HO064583 vs. TrEMBL
Match: B7ZXB7_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 3.536e-22
Identity = 53/64 (82.81%), Postives = 57/64 (89.06%), Query Frame = -1

Query:  146 GTEVVPPNSRSHTCLLSGVYIGNVKVLVRLSFGLDGPKDVAMKLTVRSDDETVSDAIHEIVASG 337
            GTEVVP N+RSHTCLLSGV+IGN+KVLVRLSFGL GP +VAMKL VRSDD  VSD IHEIVASG
Sbjct:  836 GTEVVPKNARSHTCLLSGVFIGNMKVLVRLSFGLSGPNEVAMKLAVRSDDPEVSDKIHEIVASG 899

[Close]

BLAST of HO064583 vs. TrEMBL
Match: C5WRP5_SORBI (Putative uncharacterized protein Sb01g041860 OS=Sorghum bicolor GN=Sb01g041860 PE=4 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 4.618e-22
Identity = 53/64 (82.81%), Postives = 56/64 (87.50%), Query Frame = -1

Query:  146 GTEVVPPNSRSHTCLLSGVYIGNVKVLVRLSFGLDGPKDVAMKLTVRSDDETVSDAIHEIVASG 337
            GTEVVP N+RSHTCLLSGV+IGN KVLVRLSFGL GP +VAMKL VRSDD  VSD IHEIVASG
Sbjct:  835 GTEVVPRNARSHTCLLSGVFIGNAKVLVRLSFGLSGPNEVAMKLAVRSDDSEVSDKIHEIVASG 898

[Close]

BLAST of HO064583 vs. TrEMBL
Match: B9F6L0_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_09998 PE=4 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 4.618e-22
Identity = 53/64 (82.81%), Postives = 57/64 (89.06%), Query Frame = -1

Query:  146 GTEVVPPNSRSHTCLLSGVYIGNVKVLVRLSFGLDGPKDVAMKLTVRSDDETVSDAIHEIVASG 337
            GTEVVP N+RSHTCLLSGV+IG+ KVLVRLSFGL GPK+VAMKL VRSDD  VSD IHEIVASG
Sbjct:  850 GTEVVPKNARSHTCLLSGVFIGDAKVLVRLSFGLSGPKEVAMKLAVRSDDPEVSDKIHEIVASG 913

[Close]

BLAST of HO064583 vs. TrEMBL
Match: B8AJK8_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_10600 PE=4 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 4.618e-22
Identity = 53/64 (82.81%), Postives = 57/64 (89.06%), Query Frame = -1

Query:  146 GTEVVPPNSRSHTCLLSGVYIGNVKVLVRLSFGLDGPKDVAMKLTVRSDDETVSDAIHEIVASG 337
            GTEVVP N+RSHTCLLSGV+IG+ KVLVRLSFGL GPK+VAMKL VRSDD  VSD IHEIVASG
Sbjct:  840 GTEVVPKNARSHTCLLSGVFIGDAKVLVRLSFGLSGPKEVAMKLAVRSDDPEVSDKIHEIVASG 903

[Close]

BLAST of HO064583 vs. TrEMBL
Match: B7EEW9_ORYSJ (cDNA clone:J013155G01, full insert sequence OS=Oryza sativa subsp. japonica PE=2 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 4.618e-22
Identity = 53/64 (82.81%), Postives = 57/64 (89.06%), Query Frame = -1

Query:  146 GTEVVPPNSRSHTCLLSGVYIGNVKVLVRLSFGLDGPKDVAMKLTVRSDDETVSDAIHEIVASG 337
            GTEVVP N+RSHTCLLSGV+IG+ KVLVRLSFGL GPK+VAMKL VRSDD  VSD IHEIVASG
Sbjct:  834 GTEVVPKNARSHTCLLSGVFIGDAKVLVRLSFGLSGPKEVAMKLAVRSDDPEVSDKIHEIVASG 897

[Close]

BLAST of HO064583 vs. TAIR peptide
Match: AT4G34450.1 (| Symbols: | coatomer gamma-2 subunit, putative / gamma-2 coat protein, putative / gamma-2 COP, putative | chr4:16471956-16476795 FORWARD LENGTH=886)

HSP 1 Score: 102.449 bits (254), Expect = 5.746e-23
Identity = 48/64 (75.00%), Postives = 57/64 (89.06%), Query Frame = -1

Query:  146 GTEVVPPNSRSHTCLLSGVYIGNVKVLVRLSFGLDGPKDVAMKLTVRSDDETVSDAIHEIVASG 337
            GTE +P N+RSHTCLLSGVYIGNVKVLVR  FG+D  KD+AMKLTVR++D +V++AIHEIVASG
Sbjct:  823 GTETIPLNARSHTCLLSGVYIGNVKVLVRAQFGMDSSKDIAMKLTVRAEDVSVAEAIHEIVASG 886

[Close]

BLAST of HO064583 vs. TAIR peptide
Match: AT2G16200.2 (| Symbols: | structural molecules | chr2:7024660-7025104 FORWARD LENGTH=83)

HSP 1 Score: 89.3521 bits (220), Expect = 5.034e-19
Identity = 41/64 (64.06%), Postives = 52/64 (81.25%), Query Frame = -1

Query:  146 GTEVVPPNSRSHTCLLSGVYIGNVKVLVRLSFGLDGPKDVAMKLTVRSDDETVSDAIHEIVASG 337
            GTE V  N+RSHTCL SG+YIGNVKVLV+  FG+D  K++ MKL VR++D +VSDAIH +VA+G
Sbjct:   20 GTETVASNARSHTCLPSGLYIGNVKVLVKAQFGMDSSKEIVMKLAVRAEDPSVSDAIHALVANG 83

[Close]

BLAST of HO064583 vs. TAIR peptide
Match: AT2G16200.1 (| Symbols: | structural molecules | chr2:7024766-7025104 FORWARD LENGTH=69)

HSP 1 Score: 89.3521 bits (220), Expect = 5.034e-19
Identity = 41/64 (64.06%), Postives = 52/64 (81.25%), Query Frame = -1

Query:  146 GTEVVPPNSRSHTCLLSGVYIGNVKVLVRLSFGLDGPKDVAMKLTVRSDDETVSDAIHEIVASG 337
            GTE V  N+RSHTCL SG+YIGNVKVLV+  FG+D  K++ MKL VR++D +VSDAIH +VA+G
Sbjct:    6 GTETVASNARSHTCLPSGLYIGNVKVLVKAQFGMDSSKEIVMKLAVRAEDPSVSDAIHALVANG 69

The following BLAST results are available for this feature:

BLAST of HO064583 vs. SwissProt
BLAST of HO064583 vs. TrEMBL
BLAST of HO064583 vs. TAIR peptide

BLAST of HO064583 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 3


Match Name	E-value	Identity	Description
COPG2_ORYSJ	5.912e-24	82.81	Coatomer subunit gamma-2 OS=Oryza sativa subsp. ja...	[more]
COPG1_ORYSJ	2.247e-23	82.81	Coatomer subunit gamma-1 OS=Oryza sativa subsp. ja...	[more]
COPG_ARATH	7.227e-22	75.00	Coatomer subunit gamma OS=Arabidopsis thaliana GN=...	[more]

BLAST of HO064583 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7TQ06_VITVI	1.298e-24	87.50	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9RBE3_RICCO	1.099e-23	84.38	Coatomer gamma subunit, putative OS=Ricinus commun...	[more]
B9FW22_ORYSJ	1.215e-22	82.81	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B8B874_ORYSI	1.215e-22	82.81	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B7ZZX2_MAIZE	3.536e-22	82.81	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B7ZXB7_MAIZE	3.536e-22	82.81	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
C5WRP5_SORBI	4.618e-22	82.81	Putative uncharacterized protein Sb01g041860 OS=So...	[more]
B9F6L0_ORYSJ	4.618e-22	82.81	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B8AJK8_ORYSI	4.618e-22	82.81	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B7EEW9_ORYSJ	4.618e-22	82.81	cDNA clone:J013155G01, full insert sequence OS=Ory...	[more]

BLAST of HO064583 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT4G34450.1	5.746e-23	75.00	\| Symbols: \| coatomer gamma-2 subunit, putative /...	[more]
AT2G16200.2	5.034e-19	64.06	\| Symbols: \| structural molecules \| chr2:7024660-...	[more]
AT2G16200.1	5.034e-19	64.06	\| Symbols: \| structural molecules \| chr2:7024766-...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 3..14 score
None	No IPR available	PANTHER	PTHR10261	COATOMER GAMMA SUBUNIT	coord: 3..63 score: 1
IPR009028	Clathrin/coatomer adaptor, adaptin-like, appendage, C-terminal subdomain	SUPERFAMILY	55711	Subdomain of clathrin and coatomer appendage domain	coord: 3..63 score: 2.1
IPR014863	Coatomer, gamma subunit , appendage	PFAM	PF08752	Gamma-COP	coord: 3..63 score: 3.7
IPR015873	Clathrin alpha-adaptin/coatomer adaptor, appendage, C-terminal subdomain	GENE3D	3.30.310.30	no description	coord: 3..63 score: 2.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO064583 ID=HO064583; Name=HO064583; organism=Cicer arietinum; type=EST; length=343bp
AGCGTGGTCGCGGCCGAGGTACTGATCCCAACAATTTAAGTTTTGTATTT
CTTTACACACATCTATCTATGTGCCTGCTTAACTCCAATCTCATTAACAA
AAACAGGGACCACATTGAGTCAAAATTTATCTACTTAAAAGATTATCCGC
TAGCTACAATCTCATGAATGGCATCGCTGACAGTTTCATCGTCGGATCTG
ACAGTCAGTTTCATTGCAACATCTTTTGGACCATCAAGTCCAAAAGACAA
CCGCACAAGCACCTTTACATTACCTATGTATACACCCGACAATAAACATG
TGTGTGATCTTGAGTTGGGTGGAACAACCTCTGTACCTGCCCG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0005198	structural molecule activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006886	intracellular protein transport
GO:0016192	vesicle-mediated transport

Vocabulary: **Cellular Component**
Term	Definition
GO:0030117	membrane coat
GO:0005798	Golgi-associated vesicle

Vocabulary: **INTERPRO**
Term	Definition
IPR009028	Coatomer/calthrin_app_sub_C
IPR014863	Coatomer, gamma subunit , appendage
IPR015873	Clathrin_a/coatomer_app_sub_C

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